CN102357128A - Application of extractive of Chinese herbal medicine cortex moutan to prepare weight-losing lipid-lowering medicines or medicines with effects of inhibiting lipase activity - Google Patents
Application of extractive of Chinese herbal medicine cortex moutan to prepare weight-losing lipid-lowering medicines or medicines with effects of inhibiting lipase activity Download PDFInfo
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Abstract
The invention discloses application of alcohol extractive of Chinese herbal medicine cortex moutan to prepare weight-losing lipid-lowering medicines or medicines with effects of inhibiting lipase activity, which resolves the problems of low efficiency and large side-effect of existing clinical weight-losing medicines, particularly weight-losing Chinese medicines. The weight-losing lipid-lowering medicines capable of having effects of clear mechanism, scientific target spot, small side-effect and high clinical effective rate are provided.
Description
Technical field
The present invention relates to the Chinese herbal medicine Cortex Moutan extract and have the application in the medicine of lipase activity inhibition in preparation Weight-lossing hypolipemic medicine or preparation.
Background technology
(Metabolic syndrome MS) mainly comprises risk factors such as obesity, impaired glucose tolerance, metabolism disorder of blood lipid, hypertension to metabolism syndrome.MS patient's diabetes (Diabetes mellitus, DM), the sickness rate of cardiovascular disease often is the several times of normal population.MS is the human physical and mental health of serious harm, the public health problem that becomes international, but we are very limited to the understanding of MS so far.Present preliminary research confirms that in numerous causes of disease, fat relation with MS is particularly close, is MS morbidity, development key factor and core link.Along with the raising of expanding economy, people's living standard and the change of life style, the fat maximum chronic disease of global adult that become.
At present, U.S. FDA has only been ratified 2 kinds of medicines and can be used for bariatrician for a long time: sibutramine (Sibutramine) and orlistat (Orlistat).Sibutramine is a kind of monoamine neurotransmitter reuptake inhibitor; It acts on the central nervous system and ingests with minimizing and reduce body weight; But this medicine possibly cause patient's hypertension, heart rate and accelerate; And the patient that can not be used for medical histories such as coronary heart disease, arrhythmia and apoplexy uses this medicine and is restricted.Orlistat is a kind of pancreatic lipase inhibitor, can suppress the activity of pancreatic lipase in the gastrointestinal tract, and it is about 30% that fat absorption is reduced, and its systemic side effects is starkly lower than other existing appetrol, is a successful example of lipase inhibitor.
Chinese herbal medicine is the natural pharmaceutical resources of the unique advantage of China, and classic of TCM record at all times and clinical practice all have the instance of a large amount of Chinese herbal medicine Weight-reducing and lipid-lowerings.But because the Chinese herbal medicine active component is indeterminate, pharmacological mechanism does not know and waits restriction that traditional Chinese herbal medicine can't be gone to the world, and China does not also have the slimming medicine of independent intellectual property right so far.Therefore screening has the effective ingredient of lipase activity inhibition from Chinese herbal medicine, and carries out the development of Weight-lossing hypolipemic medicine, is main purpose of the present invention.
Summary of the invention
The objective of the invention is to open Chinese herbal medicine Cortex Moutan extract and have the application in the medicine of lipase activity inhibition in preparation Weight-lossing hypolipemic medicine or preparation.
The present invention seeks to realize through following technical scheme:
The water extract of following Chinese herbal medicine or alcohol extract all have lipase activity inhibition:
Radix Scutellariae; Folium camelliae assamicae; Oolong tea; TIEGUANYIN tea; Folium Ilicis; Black tea; Green tea; CitrusaurantiumL.Var.amara Engl.; Flos Carthami; Pericarpium Zanthoxyli; Aloe; Rhizoma Polygoni Cuspidati; Folium Sennae; Radix Puerariae; Radix Salviae Miltiorrhizae; The Rhizoma Atractylodis Macrocephalae; Flos persicae; Herba Apocyni veneti; Flos Chrysanthemi; Flos Campsis; Flos Jasmini Sambac; Semen Oroxyli; Semen Vitis viniferae; Flos Trollii; Flos Caryophylli; Flos Lonicerae; Radix Cynanchi Atrati; Radix Sophorae Flavescentis; Herba Cassiae Nomamis; Folium Artemisiae Argyi; Radix Ginseng; The Flos Sophorae Immaturus; Rhizoma Fagopyri Dibotryis; Rhizoma Alismatis; Fructus Aurantii; Fructus Aurantii Immaturus; Radix Achyranthis Bidentatae; The Fructus Chebulae; Caulis Polygoni Multiflori; The Radix Aucklandiae; Folium Ginkgo; Rhizoma Osmundae; The Cortex Eucommiae; Pollen Tyjphae; Fructus Trichosanthis; Folium Mori; Cortex Mori;
The method for preparing of said herbal medicine water extract is following: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume deionized water doubly, boiled 30-60 minute, filtration or centrifugal is got filtrating or supernatant and is promptly got extract; The method for preparing of said herbal medicine alcohol extract is following: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume 50%-95% ethanol doubly, supersound extraction 30-90 minute, filtration or centrifugal was got filtrating or supernatant and is promptly got extract, gets supernatant and promptly gets extract;
The preferred for preparation method of said herbal medicine water extract is following: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 45 minutes, filtration or centrifugal is got filtrating or supernatant and is promptly got extract; The preferred for preparation method of said herbal medicine alcohol extract is following: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filtration or centrifugal is got filtrating or supernatant and is promptly got extract.
The alcohol extract of following Chinese herbal medicine has lipase activity inhibition:
Radix Et Rhizoma Rhei, Radix Glycyrrhizae, Rhizoma Chuanxiong, Fructus Chaenomelis, Caulis Spatholobi, Cortex Magnoliae Officinalis, Cortex Moutan, Herb Gynostemmae Pentaphylli, Rhizoma Curcumae Longae, Rhizoma Et Radix Notopterygii, Radix Polygoni Multiflori, Semen Arecae, Ramulus Cinnamomi;
The method for preparing of said herbal medicine alcohol extract is following: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume 50%-95% ethanol doubly, supersound extraction 30-90 minute, filtration or centrifugal was got filtrating or supernatant and is promptly got extract;
The preferred for preparation method of said herbal medicine alcohol extract is following: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, filtration or centrifugal is got filtrating or supernatant and is promptly got extract.
The water extract of following Chinese herbal medicine has lipase activity inhibition:
Fructus Lycii, Folium Nelumbinis, Radix Platycodonis;
The preferred for preparation method of said herbal medicine water extract is following: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume deionized water doubly, boiled 30-60 minute, filtration or centrifugal is got filtrating or supernatant and is promptly got extract;
The preferred for preparation method of said herbal medicine water extract is following: get above-mentioned each single herbal decoction piece, and prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 45 minutes, filtration or centrifugal is got filtrating or supernatant and is promptly got extract.
But said herbal medicine extract of the present invention is all single uses in the application of preparation Weight-lossing hypolipemic medicine, use also capable of being combined, and Weight-lossing hypolipemic medicine can be processed by water, alcohol and the extractive with organic solvent of said herbal medicine or the effective ingredient of other method purification;
Weight-lossing hypolipemic medicine can also comprise the active components playing weight losing and hypolipemic role of following one or more other mechanisms of action: glycosidase inhibitor, fatty acid synthetase inhibitor, cholesterol synthase inhibitor, amylase inhibitor;
In order to increase drug effect or to reduce side effect, Weight-lossing hypolipemic medicine can also comprise the Chinese herbal medicine of following one or more other pharmacological actions: Fructus Corni, Herba Ephedrae, Folium Isatidis, Herba Taraxaci, Radix Rehmanniae Preparata;
Weight-lossing hypolipemic medicine can also comprise following one or more excipient: Radix Acaciae senegalis, skim milk, mannan, glucomannan, Rhizoma amorphophalli powder, starch, lipotropy sclerosing agent;
Weight-lossing hypolipemic medicine can also comprise following one or more pharmaceutic adjuvants or reduce the existing product of drug side effect: Radix Acaciae senegalis, skim milk, mannan, glucomannan, Rhizoma amorphophalli powder, starch, lipotropy sclerosing agent, stearic acid.
Above-mentioned lipase refers in particular to pancreatic lipase.
The present invention is a target spot with the pancreatic lipase activity, is index with the degree of pancreatic lipase activity inhibited, from a large amount of Chinese herbal medicine; Screen said herbal medicine; Its extract all has the effect of obvious suppression lipase active, and it is low to the invention solves existing clinical slimming medicine (especially Chinese medicine) effective percentage, the shortcoming that side effect is big; Provide a kind of mechanism clear and definite, target spot science, the Weight-lossing hypolipemic medicine that side effect is little, clinical effective rate is high.
Following experimental example and embodiment are used to further specify but are not limited to the present invention.
The water extract of experimental example 1 Chinese herbal medicine of the present invention and alcohol extract are tested the lipase inhibitory action
1, the water extract or the alcohol extract of preparation Chinese herbal medicine of the present invention:
The water extract preparation: get each single herbal decoction piece, prefabricated, pulverize; Add 5 volumes deionized water doubly, boiled 30 minutes, filtration or centrifugal is got filtrating or supernatant and is promptly got extract;
The ethanol extraction preparation: get each single herbal decoction piece, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 60 minutes, filtration or centrifugal is got filtrating or supernatant and is promptly got extract.
2, the mensuration of lipase activity inhibition rate:
A. prepare reagent, olive oil emulsion and measure enzyme liquid:
The preparation of olive oil emulsion: get olive oil 12ml and Radix Acaciae senegalis 22.5g, grind evenly, add water and be ground to 300ml, with the homogenizer emulsifying of 18000r/min 2 times, each 3 minutes; Get Emulsion at test under microscope, the diameter of 90% breast grain and must not have the breast grain of 10 μ m below 3 μ m, get final product;
The preparation of bovine bile solution: precision takes by weighing bovine bile 8.0g, places the 50ml beaker, with water dissolution, is transferred to standardize solution in the 100ml volumetric flask;
0.1mol/L the preparation of sodium hydroxide solution: accurate weighing sodium hydroxide 2.0g, being dissolved in water is settled to 500ml;
TRIS buffer preparation: get Tris 3.030g,, add water to 500ml, shake up re-adjustment pH value to 8.0 with 0.1mol/L hydrochloric acid solution adjust pH to 8.0;
The preparation of lipase solution: precision takes by weighing pancreatic lipase (SIGMA:L3126) in volumetric flask, with the TRIS buffer dissolving that is cooled to below 5 ℃, processes the solution that contains pancreatic lipase 100 unit of activity among every 1ml, and is subsequent use as proenzyme liquid; During the drawing standard curve, proenzyme liquid is diluted to the mensuration enzyme liquid of different gradient 90u/ml, 80u/ml, 70u/ml, 60u/ml, 50u/ml, 40u/ml, 30u/ml, 20u/ml, 10u/ml with TRIS buffer; The mensuration enzyme liquid of 0u/ml, that is: enzyme is blank, is the proenzyme liquid that in water-bath, boiled 15-30 minute;
B. set up lipase and survey live body system:
1). water is that sample blank solution lipase is surveyed live body system: get olive oil emulsion 25ml, 8% bovine bile solution 2ml and water 10ml, put in the 100ml triangular flask, add water 2ml, regulate pH value to 9.0 with the 0.1mol/L sodium hydroxide solution;
2) .75% ethanol is that sample blank solution lipase is surveyed live body system: get olive oil emulsion 25ml, 8% bovine bile solution 2ml and water 10ml, put in the 100ml triangular flask, add 75% alcoholic solution 2ml, regulate pH value to 9.0 with the 0.1mol/L sodium hydroxide solution;
3). the sample solution lipase is surveyed live body system: get olive oil emulsion 25ml, 8% bovine bile solution 2ml and water 10ml, put in the 100ml triangular flask, add sample solution 2ml respectively, regulate pH value to 9.0 with the 0.1mol/L sodium hydroxide solution; Sample solution can be water extract or alcohol extract a kind of of 63 kinds of Chinese herbal medicine.
C. draw lipase suppression ratio " Y% " and concern standard curve with pH amplitude of variation " Δ pH ":
During the drawing standard curve, the mensuration enzyme liquid with different gradients is converted into corresponding suppression ratio, the suppression ratio reduction formula earlier:
Y(％)＝(X
0-Xn)/X
0
Y (%)-expression lipase suppression ratio
X
0-expressed enzyme original liquid concentration (u/ml)
Xn-representes different dilution enzyme liquid concentration (u/ml);
When the setting enzyme concentration was zero, suppression ratio was 100%; The suppression ratio of setting proenzyme liquid concentration is 0; Proenzyme liquid is become the mensuration enzyme liquid of 90u/ml, 80u/ml, 70u/ml, 60u/ml, 50u/ml, 40u/ml, 30u/ml, 20u/ml, 10u/ml by certain gradient dilution, and then corresponding suppression ratio Y% is respectively 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%; Draw method drawing standard curve according to standard curve, standard curve drafting method of the present invention is following:
Above-mentioned water or 75% ethanol blank solution lipase are surveyed live body tied up in 37 ℃ of water-baths insulation 10 minutes, with 0.1mol/L sodium hydroxide solution adjusting pH value to 9.0; Precision is measured mensuration enzyme liquid 1 parts by volume of above-mentioned different gradient concentrations, adds to blank solution respectively and surveys in the live body system, and accurate response is 5 minutes in 37 ℃ of water-baths, writes down reaction front and back pH value simultaneously, and calculates reaction front and back pH difference; PH difference when using enzyme concentration before and after the reaction as 100u/ml; PH difference when deducting enzyme concentration respectively and being 90u/ml, 80u/ml, 70u/ml, 60u/ml, 50u/ml, 40u/ml, 30u/ml, 20u/ml, 10u/ml, 0u/ml before and after the reaction promptly obtains pH amplitude of variation " Δ pH " value of corresponding different suppression ratio; With the suppression ratio is vertical coordinate, and " Δ pH " value is drawn lipase suppression ratio " Y% " and concerned standard curve with pH amplitude of variation " Δ pH " for abscissa, and obtains water or 75% and be the lipase suppression ratio computing formula of blank sample solution;
D. the mensuration of assay method and sample solution lipase suppression ratio
Blank solution is measured: above-mentioned water or 75% ethanol blank solution lipase are surveyed live body tied up in 37 ℃ of water-baths insulation 10 minutes, with 0.1mol/L sodium hydroxide solution adjusting pH value to 9.0; Precision is measured proenzyme liquid 1ml and is added in the blank solution survey live body system, and accurate response is 5 minutes in 37 ℃ of water-baths, and pH value before and after the record reaction simultaneously, and the pH difference before and after the reaction of calculating blank solution are designated as A1; Other is taken at the above-mentioned proenzyme liquid 1ml that boiled in the water-bath 15-30 minute, measures according to said method, and as the enzyme blank, the pH difference is designated as A0; Then the pH changing value of blank solution enzyme reaction is Δ A=A1-A0;
Sample solution is measured: sample solution lipase survey live body is tied up in 37 ℃ of water-baths be incubated 10 minutes, regulate pH value to 9.0 with the 0.1mol/L sodium hydroxide solution; Precision is measured proenzyme liquid 1ml and is added in the sample solution survey live body system, and accurate response is 5 minutes in 37 ℃ of water-baths, and pH value before and after the record reaction simultaneously, and the pH difference of calculation sample solution reaction front and back are designated as B1; Other is taken at the above-mentioned proenzyme liquid 1ml that boiled in the water-bath 20 minutes, measures according to said method, and as the enzyme blank, the pH difference is designated as B0; Then the pH changing value of sample solution enzyme reaction is Δ B=B1-B0;
The lipase suppression ratio of sample solution: the pH changing value (Δ B) that deducts the sample solution enzyme reaction with the pH changing value (Δ A) of blank solution enzyme reaction; Promptly obtain " Δ pH " value of blank solution and sample solution enzyme reaction; Be Δ pH=Δ A-Δ B; With the lipase suppression ratio computing formula of the above-mentioned corresponding blank solution of this " Δ pH " value substitution, promptly try to achieve the lipase suppression ratio of working sample, the lipase suppression ratio result of sample solution sees table 1:
Table 1 Chinese herbal medicine extract is to lipase activity inhibition rate
The power how many representatives of "+" suppress lipase, "+" is many more, and expression suppresses strong more to lipase.
Following embodiment all can realize the effect of above-mentioned experimental example.
The specific embodiment
Embodiment 1: the preparation of Radix Scutellariae water extract
Get baikal skullcap root decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boiled 30 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 2: the preparation of Radix Scutellariae alcohol extract
Get baikal skullcap root decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 3: the preparation of Folium camelliae assamicae water extract
Get the Folium camelliae assamicae decoction pieces, prefabricated, pulverize; Add 10 volumes deionized water doubly, boiled 60 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 4: the preparation of Folium camelliae assamicae alcohol extract
Get the Folium camelliae assamicae decoction pieces, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 90 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 5: the preparation of oolong tea water extract
Get the oolong tea decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 6: the preparation of oolong tea alcohol extract
Get the oolong tea decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 30 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 7: the preparation of TIEGUANYIN tea water extract
Get the TIEGUANYIN tea decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 8: the preparation of TIEGUANYIN tea alcohol extract
Get the TIEGUANYIN tea decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 50 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 9: the preparation of Folium Ilicis water extract
Get the Folium Ilicis decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 10: the preparation of Folium Ilicis alcohol extract
Get the Folium Ilicis decoction pieces, prefabricated, pulverize; Add 7 volumes, 50% ethanol doubly, supersound extraction 80 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 11: the preparation of black tea water extract
Get the black tea decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 12: the preparation of black tea alcohol extract
Get the black tea decoction pieces, prefabricated, pulverize; Add 7 volumes, 70% ethanol doubly, supersound extraction 70 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 13: the preparation of green tea water extract
Get the green tea decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 14: the preparation of green tea alcohol extract
Get the green tea decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 50 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 15: the preparation of CitrusaurantiumL.Var.amara Engl. water extract
Replace the seville orange flower decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 16: the preparation of CitrusaurantiumL.Var.amara Engl. alcohol extract
Replace the seville orange flower decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 40 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 17: the preparation of Flos Carthami water extract
Get the Flos Carthami decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boiled 60 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 18: the preparation of Flos Carthami alcohol extract
Get the Flos Carthami decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 30 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 19: the preparation of pepper water extract
Get the Pericarpium Zanthoxyli decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 45 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 20: the preparation of Pericarpium Zanthoxyli alcohol extract
Get the Pericarpium Zanthoxyli decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 21: the preparation of aloe water extract
Get the Aloe decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boiled 30 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 22: the preparation of Aloe alcohol extract
Get the Aloe decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 85 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 23: the preparation of Rhizoma Polygoni Cuspidati water extract
Get the Rhizoma Polygoni Cuspidati decoction pieces, prefabricated, pulverize; Add 10 volumes deionized water doubly, boiled 60 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 24: the preparation of Rhizoma Polygoni Cuspidati alcohol extract
Get the Rhizoma Polygoni Cuspidati decoction pieces, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 75 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 25: the preparation of Folium Sennae water extract
Get the Folium Sennae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, filter, get supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 26: the preparation of Folium Sennae alcohol extract
Get the Folium Sennae decoction pieces, prefabricated, pulverize; Add 6 volumes, 60% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 27: the preparation of Radix Puerariae water extract
Get the Radix Puerariae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, 4000rpm/ minute centrifugal, gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 28: the preparation of Radix Puerariae alcohol extract
Get the Radix Puerariae decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 80 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 29: the preparation of Radix Salviae Miltiorrhizae water extract
Get salvia piece, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 30: the preparation of tanshinol extract
Get salvia piece, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 31: the preparation of Rhizoma Atractylodis Macrocephalae water extract
Get Rhizoma Atractylodis Macrocephalae decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 32: the preparation of Rhizoma Atractylodis Macrocephalae alcohol extract
Get Rhizoma Atractylodis Macrocephalae decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 70 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 33: the preparation of Flos persicae water extract
Get the Flos persicae decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 34: the preparation of Flos persicae alcohol extract
Get the Flos persicae decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 50 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 35: the preparation of Herba Apocyni veneti water extract
Get the Herba Apocyni veneti decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 36: the preparation of Herba Apocyni veneti alcohol extract
Get the Herba Apocyni veneti decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 60 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 37: the preparation of Flos Chrysanthemi water extract
Get the Flos Chrysanthemi decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boiled 60 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 38: the preparation of Flos Chrysanthemi alcohol extract
Get the Flos Chrysanthemi decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 65 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 39: the preparation of Flos Campsis water extract
Get the Flos Campsis decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 45 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 40: the preparation of Flos Campsis alcohol extract
Get the Flos Campsis decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 41: the preparation of Flos Jasmini Sambac water extract
Get the Flos Jasmini Sambac decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boiled 30 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 42: the preparation of Flos Jasmini Sambac alcohol extract
Get the Flos Jasmini Sambac decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 45 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 43: the preparation of Semen Oroxyli water extract
Get the Semen Oroxyli decoction pieces, prefabricated, pulverize; Add 10 volumes deionized water doubly, boiled 60 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 44: the preparation of Semen Oroxyli alcohol extract
Get the Semen Oroxyli decoction pieces, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 45: the preparation of Semen Vitis viniferae water extract
Get the Semen Vitis viniferae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 46: the preparation of Semen Vitis viniferae alcohol extract
Get the Semen Vitis viniferae decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 90 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 47: the preparation of Flos Trollii water extract
Depletion Flos Nelumbinis decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 48: the preparation of Flos Trollii alcohol extract
Depletion Flos Nelumbinis decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 49: the preparation of Flos Caryophylli water extract
Get the Flos Caryophylli decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 50: the preparation of syringyl alcohol extract
Get the Flos Caryophylli decoction pieces, prefabricated, pulverize; Add 7 volumes, 75% ethanol doubly, supersound extraction 75 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 51: the preparation of Flos Lonicerae water extract
The extracting honeysuckle decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 52: the preparation of Flos Lonicerae alcohol extract
The extracting honeysuckle decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 53: the preparation of Radix Cynanchi Atrati water extract
Get the Radix Cynanchi Atrati decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 54: the preparation of Radix Cynanchi Atrati alcohol extract
Get the Radix Cynanchi Atrati decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 70 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 55: the preparation of Radix Sophorae Flavescentis water extract
Get the Radix Sophorae Flavescentis decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 56: the preparation of Radix Sophorae Flavescentis alcohol extract
Get the Radix Sophorae Flavescentis decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 80 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 57: the preparation of Herba Cassiae Nomamis water extract
Water intaking Fructus Gleditsia decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boiled 60 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 58: the preparation of Herba Cassiae Nomamis alcohol extract
Water intaking Fructus Gleditsia decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 59: the preparation of Folium Artemisiae Argyi water extract
Get the Folium Artemisiae Argyi decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 45 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 60: the preparation of Folium Artemisiae Argyi alcohol extract
Get the Folium Artemisiae Argyi decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 61: the preparation of Radix Ginseng water extract
Get the Radix Ginseng decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boiled 30 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 62: the preparation of panaxynol extract
Get the Radix Ginseng decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 63: the preparation of Flos Sophorae Immaturus water extract
Get Flos Sophorae Immaturus decoction pieces, prefabricated, pulverize; Add 10 volumes deionized water doubly, boiled 60 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 64: the preparation of Flos Sophorae Immaturus alcohol extract
Get Flos Sophorae Immaturus decoction pieces, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 70 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 65: the preparation of Rhizoma Fagopyri Dibotryis water extract
Depletion Semen Fagopyri Esculenti decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 66: the preparation of Rhizoma Fagopyri Dibotryis alcohol extract
Depletion Semen Fagopyri Esculenti decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 67: the preparation of Rhizoma Alismatis water extract
Get the Rhizoma Alismatis decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 68: the preparation of alisol extract
Get the Rhizoma Alismatis decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 69: the preparation of Fructus Aurantii water extract
Get the Fructus Aurantii decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 70: the preparation of Fructus Aurantii alcohol extract
Get the Fructus Aurantii decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 71: the preparation of Radix Achyranthis Bidentatae water extract
Get the Radix Achyranthis Bidentatae decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 72: the preparation of Radix Achyranthis Bidentatae alcohol extract
Get the Radix Achyranthis Bidentatae decoction pieces, prefabricated, pulverize; Add 7 volumes, 65% ethanol doubly, supersound extraction 75 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 73: the preparation of Fructus Aurantii Immaturus water extract
Get the Fructus Aurantii Immaturus decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 74: the preparation of Fructus Aurantii Immaturus alcohol extract
Get the Fructus Aurantii Immaturus decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 75: the preparation of Fructus Chebulae's water extract
Get Fructus Chebulae's decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 76: the preparation of Fructus Chebulae's alcohol extract
Get Fructus Chebulae's decoction pieces, prefabricated, pulverize; Add 8 volumes, 90% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 77: the preparation of Caulis Polygoni Multiflori water extract
Get the Caulis Polygoni Multiflori decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boiled 60 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 78: the preparation of Caulis Polygoni Multiflori alcohol extract
Get the Caulis Polygoni Multiflori decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 79: the preparation of Radix Aucklandiae water extract
Get Radix Aucklandiae decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 45 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 80: the preparation of costol extract
Get Radix Aucklandiae decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 50 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 81: the preparation of Folium Ginkgo water extract
Get the Folium Ginkgo decoction pieces, prefabricated, pulverize; Add 5 volumes deionized water doubly, boiled 30 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 82: the preparation of Folium Ginkgo alcohol extract
Get the Folium Ginkgo decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 45 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 83: the preparation of Rhizoma Osmundae water extract
Get the Rhizoma Osmundae decoction pieces, prefabricated, pulverize; Add 10 volumes deionized water doubly, boiled 60 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 84: the preparation of Rhizoma Osmundae alcohol extract
Get the Rhizoma Osmundae decoction pieces, prefabricated, pulverize; Add 10 volumes, 50% ethanol doubly, supersound extraction 60 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 85: the preparation of Cortex Eucommiae water extract
Get Cortex Eucommiae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 86: the preparation of eucommoil extract
Get Cortex Eucommiae decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 87: the preparation of Pollen Tyjphae water extract
Get the Pollen Tyjphae decoction pieces, prefabricated, pulverize; Add 6 volumes deionized water doubly, boiled 40 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 88: the preparation of Pollen Tyjphae alcohol extract
Get the Pollen Tyjphae decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 50 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 89: the preparation of Fructus Trichosanthis water extract
Get the Fructus Trichosanthis decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 90: the preparation of Fructus Trichosanthis alcohol extract
Get the Fructus Trichosanthis decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 91: the preparation of Folium Mori water extract
Get the Folium Mori decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 50 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 92: the preparation of Folium Mori alcohol extract
Get the Folium Mori decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 93: the preparation of Cortex Mori water extract
Get the Cortex Mori decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 94: the preparation of Cortex Mori alcohol extract
Get the Cortex Mori decoction pieces, prefabricated, pulverize; Add 8 volumes, 90% ethanol doubly, supersound extraction 80 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 95: the preparation of Fructus Lycii water extract
Get the Fructus Lycii decoction pieces, prefabricated, pulverize; Add 8 volumes deionized water doubly, boiled 50 minutes, centrifugal 20 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 96: the preparation of Folium Nelumbinis water extract
Get the Folium Nelumbinis decoction pieces, prefabricated, pulverize; Add 9 volumes deionized water doubly, boiled 60 minutes, filter, get filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 97: the preparation of Radix Platycodonis water extract
Get the Radix Platycodonis decoction pieces, prefabricated, pulverize; Add 7 volumes deionized water doubly, boiled 45 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 98: the preparation of rhababerone extract
Get Radix Et Rhizoma Rhei decoction piece, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 75 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 99: the preparation of glycyrol extract
The extracting liquorice decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 100: the preparation of Rhizoma Chuanxiong alcohol extract
Get the Rhizoma Chuanxiong decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 70 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 101: the preparation of Fructus Chaenomelis alcohol extract
Get the Fructus Chaenomelis decoction pieces, prefabricated, pulverize; Add 5 volumes, 75% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 102: the preparation of milletol extract
Get the Caulis Spatholobi decoction pieces, prefabricated, pulverize; Add 10 volumes, 95% ethanol doubly, supersound extraction 60 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 103: the preparation of Cortex Magnoliae Officinalis alcohol extract
Get the Cortex Magnoliae Officinalis decoction pieces, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 104: the preparation of Cortex Moutan alcohol extract
Get paeonol piece, prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 105: the preparation of Herb Gynostemmae Pentaphylli alcohol extract
Get the Herb Gynostemmae Pentaphylli decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 106: the preparation of curcumol extract
Get the Rhizoma Curcumae Longae decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 90 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 107: the preparation of Notopterol extract
Get the Rhizoma Et Radix Notopterygii decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 108: the preparation of Radix Polygoni Multiflori alcohol extract
Get the Radix Polygoni Multiflori decoction pieces, prefabricated, pulverize; Add 8 volumes, 95% ethanol doubly, supersound extraction 90 minutes, centrifugal 10 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 109: the preparation of Semen Arecae alcohol extract
Get the Semen Arecae decoction pieces, prefabricated, pulverize; Add 9 volumes, 95% ethanol doubly, supersound extraction 60 minutes is filtered, and gets filtrating; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
Embodiment 110: the preparation of Ramulus Cinnamomi alcohol extract
Get the Ramulus Cinnamomi decoction pieces, prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 15 minutes of 4000r/min gets supernatant; Process regular dosage form with conventional method, be used to prepare lipase inhibitor.
The above is merely the preferred embodiments of the present invention, is not limited to the present invention, and for a person skilled in the art, the present invention can have various changes and variation.All within spirit of the present invention and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. the alcohol extract of a Chinese herbal medicine Cortex Moutan has the application in the medicine of lipase activity inhibition in preparation Weight-lossing hypolipemic medicine or preparation.
2. application according to claim 1 is characterized in that, the alcohol extract of said Chinese herbal medicine Cortex Moutan is prepared by following method: get the Cortex Moutan herbal decoction piece, and prefabricated, pulverize; Add 5-10 volume 50%-95% ethanol doubly, supersound extraction 30-90 minute, filter, or the centrifugal 10-20 of 4000r/min minute, get filtrate or centrifuged supernatant and promptly get said alcohol extract.
3. application according to claim 2 is characterized in that, the alcohol extract of said Chinese herbal medicine Cortex Moutan is prepared by following method: get said Cortex Moutan herbal decoction piece, and prefabricated, pulverize; Add 7 volumes, 85% ethanol doubly, supersound extraction 60 minutes is filtered, or centrifugal 15 minutes of 4000r/min, gets filtrate or centrifuged supernatant and promptly gets said alcohol extract.
4. application according to claim 2 is characterized in that, the alcohol extract of said Chinese herbal medicine Cortex Moutan is prepared by following method: get said Cortex Moutan herbal decoction piece, and prefabricated, pulverize; Add 6 volumes, 85% ethanol doubly, supersound extraction 60 minutes, centrifugal 15 minutes of 4000r/min gets supernatant and promptly gets said alcohol extract.
5. according to each described application of claim 1-4, it is characterized in that said lipase is a pancreatic lipase.
6. according to each described application of claim 1-4, it is characterized in that, comprise glycosidase inhibitor, fatty acid synthetase inhibitor, cholesterol synthase inhibitor or amylase inhibitor in the said Weight-lossing hypolipemic medicine.
7. according to each described application of claim 1-4, it is characterized in that, comprise Fructus Corni, Herba Ephedrae, Folium Isatidis, Herba Taraxaci or Radix Rehmanniae Preparata in the said Weight-lossing hypolipemic medicine.
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|---|---|---|---|
| CN2011103176543A CN102357128A (en) | 2007-08-07 | 2007-08-07 | Application of extractive of Chinese herbal medicine cortex moutan to prepare weight-losing lipid-lowering medicines or medicines with effects of inhibiting lipase activity |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011103176543A CN102357128A (en) | 2007-08-07 | 2007-08-07 | Application of extractive of Chinese herbal medicine cortex moutan to prepare weight-losing lipid-lowering medicines or medicines with effects of inhibiting lipase activity |
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| CN2007101200147A Division CN101361886B (en) | 2007-08-07 | 2007-08-07 | Chinese herbal medicine extract with lipase activity inhibition and new use thereof |
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| CN110279757A (en) * | 2019-05-27 | 2019-09-27 | 兰溪市立顺生物有限公司 | Weight reducing compound and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110279757A (en) * | 2019-05-27 | 2019-09-27 | 兰溪市立顺生物有限公司 | Weight reducing compound and preparation method thereof |
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