CN102352325B - Mutant strain ZN0871v11 and purpose thereof in rapid and efficient decolouring treatment of printing and dyeing waste water - Google Patents

Mutant strain ZN0871v11 and purpose thereof in rapid and efficient decolouring treatment of printing and dyeing waste water Download PDF

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CN102352325B
CN102352325B CN201110173958A CN201110173958A CN102352325B CN 102352325 B CN102352325 B CN 102352325B CN 201110173958 A CN201110173958 A CN 201110173958A CN 201110173958 A CN201110173958 A CN 201110173958A CN 102352325 B CN102352325 B CN 102352325B
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zn0871v11
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祝伟
曹霞
察冬梅
陈胜慧
李步海
孙小梅
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South Central Minzu University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/308—Dyes; Colorants; Fluorescent agents
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/30—Nature of the water, waste water, sewage or sludge to be treated from the textile industry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

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Abstract

The invention discloses a mutagenicity screening method for a mutant strain ZN0871v11 for rapid and efficient decolouring treatment of printing and dyeing waste water and a novel technical method of the mutant strain ZN0871v11 for rapid and efficient decolouring treatment of printing and dyeing waste water. After genetic mutation of original bacterial strains, the mutant strain ZN0871v11 is screened out; the mutant strain ZN0871v11 has rapid, efficient and excellent decolouring effect on dyes such as benzo fast blue; and a decolouring rate is raised by 80% to 100%.

Description

Mutant strain ZN0871v11 and the purposes in dyeing waste water rapidly and efficiently decolours processing thereof
Technical field
The invention belongs to the treatment of dyeing wastewater field, be specifically related to the new technology that a kind of mutant strain is rapidly and efficiently handled dyeing waste water.
Background technology
Textile industry is the important component part of China's economy, to the raising of development and national economy and living standards of the people, plays a part very important., textile industry has also produced a large amount of reluctant dyeing waste waters when increasing GDP.Dyeing industry belongs to high water consumption and high pollution industry, and " for the first time national source of pollution generaI investigation communique " shows: 2007 annual China dyeings year water loss above 10,000,000,000 tons, be about 3 times of developed country, wherein 80% one 90% discharge with dyeing waste water.In the every profession and trade of China's industry, the COD quantity discharged of textile printing and dyeing industry occupies the 4th, and wastewater discharge occupies the 5th.The organic pollutant content of dyeing waste water increases, colourity deepens, alkalescence becomes greatly, composition complicates, and the intractability of waste water increases.In addition, along with novel dyestuff application, dyeing and printing process and shift in product mix, variation has also taken place in the composition of dyeing waste water, has further strengthened the intractability of waste water.Will cause the water pollution of rivers and lakes and the destruction of ecotope and dyeing water treatment is improper., present waste water treatment process long operational time, the expense of utilizing is higher, water outlet often be difficult to up to standard, when especially the water yield is big.Biological material, new technology that the efficient decolorizing printing and dyeing waste water fast of research and development is handled can be saved the time of treatment of dyeing wastewater, reduce and pollute, and realize zero release; Improve the recovery utilization rate of water again, solved the difficult problem of lack of water; Can reclaim the organism such as dyestuff in the waste water; Can also dwindle the scale of waste water treatment plant, save the soil.Therefore, further strengthen the novel microorganism treatment process of the dyeing waste water that contains hardly degraded organic substance is had great importance to the protection environment.
Summary of the invention
We are separated to a strain bacterium Bacillus subtilis ZN0871 from typical environment, and this starting strain has been carried out ultraviolet mutagenesis.This strain bacterium has screened mutant strain ZN0871v11 through after the transgenation, and it has good decolorizing effect to dyestuffs such as sun blue, and percent of decolourization has improved 80%, has reached 100%.At Fe 2+Or Cu 2+Concentration is 20mg/100mL, in 5-10 minute, the elimination factor of sun blue, REACTIVE Red 195 is 100%, and records the COD that is caused by dyestuff and reduce to " 0 "; Reactive orange 78, REACTIVE Yellow 145, reactive yellow MS, REACTIVE Yellow 160, HFGR REACTIVE Black HFGR KN-B isoreactivity dyestuff are also had good efficient decolorizing effect fast, and percent of decolourization has reached respectively: REACTIVE Red 195 is 100%, reactive orange 78 is 69%, REACTIVE Yellow 145 is 78.8%, reactive yellow MS is 80.69%, REACTIVE Yellow 160 is 93.67%, HFGR REACTIVE Black HFGR KN-B is 96.2%.
Subtilis of the present invention is mutant strain ZN0871v11; It is the mutant strain that Bacillus subtilis ZN0871 bacterial strain screens through ultraviolet mutagenesis; Classification called after subtilis Bacillus subtilis mutant strain ZN0871v11 has been preserved in Chinese typical culture collection center on June 23rd, 2011, be called for short CCTCC; Preservation address China Wuhan Wuhan University, its preserving number is CCTCC NO:M2011208.
Therefore; One object of the present invention is to improve problems such as existing techniques of Dyeing Wastewater Treatment is consuming time, power consumption; A kind of mutant strain ZN0871v11 of treatment of dyeing and printing rapidly and efficiently is provided, and it has good efficient decolorizing effect fast to dyestuffs such as sun blue, and percent of decolourization has reached 100%.
The present invention also aims to provide a kind of microbial flocculant, said biological flocculant comprises mutant strain ZN0871v11.
Another object of the present invention is to provide the application of said mutation strain in the fast processing dyeing waste water.
When the decolouring rapidly and efficiently of dyeing waste water was handled, after mutant strain ZN0871v11 activation culture, behind cell washing 3 times, it was resuspended to add sterilized water, and cell density is 10 9Individual/mL, with the aseptic inoculation operating method, the ratio of inoculating 100 μ L bacteria suspensions with every 100mL liquid nutrient medium is got bacteria suspension and is inoculated into respectively in all kinds of dye liquid substratum.
In pH5.0, temperature is 37 ℃, metal cations Fe 2+Final concentration is under the 20mg/100mL condition, adds mutant strain ZN0871v11 cell mixing, and in 10 minutes, mutant strain ZN0871v11 is 100% to the percent of decolourization of sun blue, records the COD that is caused by dyestuff and reduces to " 0 ".
At pH7.0, temperature is 25 ℃, Fe 2+Concentration be respectively 15, under the condition of 20mg/100mL; Mutant strain ZN0871v11 is complete to the decolouring of sun blue dyestuff, in 5-10 minute, reaches maximum elimination factor; Elimination factor to the sun blue dyestuff is 100%, and records the COD that is caused by dyestuff and reduce to " 0 ".
In pH5.0, temperature is 37 ℃, Cu 2+Concentration be respectively 15, under the condition of 20mg/100mL, mutant strain ZN0871v11 is thorough to the decolouring of sun blue dyestuff, in 5-10 minute, reaches 100% elimination factor, and records the COD that is caused by dyestuff and reduce to " 0 ".
Under 20 ℃, 25 ℃, 30 ℃, 37 ℃, 42 ℃ these 5 kinds of temperature, when not adding any ion, mutant strain ZN0871v11 can reach 100% (referring to Fig. 1) to the elimination factor of sun blue when 37 ℃ and 42 ℃.Different temperature does not have too much influence to the decolouring absorption of thalline.The thermal adaptability that mutant strain ZN0871v11 is described is stronger.
Be respectively in the pH value under 10 kinds of gradients of 5.0,5.5,6.0,6.5,7.0,7.5,8.0,8.5,9.0,10.3, when not adding any ion, mutant strain ZN0871v11 cell changes greatly the decolorizing effect of sun blue dyestuff.Wherein, the decolorizing effect of sun blue under pH5.0 best (referring to Fig. 2).Under the condition of pH5.0, the elimination per-cent of sun blue dyestuff has been reached 92.8%.
At Zn 2+, Ca 2+, Mg 2+, K +, Na +Concentration be under the condition of 20mg/100mL, mutant strain ZN0871v11 is Zn to the decolorizing effect of sun blue dyestuff 2+(89.73%)＞Ca 2+(66.25%)＞Mg 2+(55.59%)＞K +(30%)＞Na +(18%).
In pH5.0, temperature is 37 ℃, metal cations Fe 2+When concentration is 20mg/100mL, carry out the absorption degradation experiment.Mutant strain ZN0871v11 is respectively the percent of decolourization of REACTIVE Red 195, reactive orange 78, REACTIVE Yellow 145, reactive yellow MS, REACTIVE Yellow 160: REACTIVE Red 195 100%, reactive orange 7869%, REACTIVE Yellow 145 78.8%, reactive yellow MS80.69%, REACTIVE Yellow 160 93.67%.
In pH6.0, temperature is 37 ℃, metal cations Fe 2+When concentration was 20mg/100mL, mutant strain ZN0871v11 was 96.2% for the percent of decolourization of HFGR REACTIVE Black HFGR KN-B.
At Cu 2+Concentration be under the condition of 20 ℃ of 20mg/100mL, pH5.0, room temperature; Mutant strain ZN0871v11 is 45.63% to the percent of decolourization of HFGR REACTIVE Black HFGR KN-B; Percent of decolourization to REACTIVE Red 195 is 59.27%, is 69% to the percent of decolourization of reactive orange 78, is 78.8% to the percent of decolourization of REACTIVE Yellow 145; Percent of decolourization to reactive yellow MS is 80.69%, is 62.24% to the percent of decolourization of REACTIVE Yellow 160.
Description of drawings
Fig. 1 has shown that temperature influences the sun blue decolorizing effect.Fig. 1 is presented under 20 ℃, 25 ℃, 30 ℃, 37 ℃, 42 ℃ these 5 kinds of temperature, and when not adding any ion, mutant strain ZN0871v11 can reach 100% to the elimination factor of sun blue when 37 ℃ and 42 ℃.
Fig. 2 has shown that pH influences the sun blue decolorizing effect.Fig. 2 is presented at the pH value and is respectively under 10 kinds of gradients of 5.0,5.5,6.0,6.5,7.0,7.5,8.0,8.5,9.0,10.3, and when not adding any ion, mutant strain ZN0871v11 cell changes greatly the decolorizing effect of sun blue dyestuff.Wherein, the decolorizing effect of sun blue under pH5.0 is best.
Embodiment
Below in conjunction with accompanying drawing preferred implementation of the present invention is described, should be appreciated that following embodiment only is to be used to explain the present invention, but not limitation of the present invention.
Embodiment 1 operation steps of the present invention
The first step, the bacterial strain activation.Will be from the South Lake water sample separate the starting strain ZN0871 that obtains and be inoculated into liquid LB substratum (peptone 10g/L with 1% inoculum size; Yeast extract 5g/L; NaCl 5g/L, pH7.0) the middle 10h that cultivates carries out activation, is transferred to afterwards in the fresh liquid LB substratum again and cultivates 10h.
Second step, mutagenesis.Open uv lamp (15W) preheating 20min, the bacterium liquid 3mL after the activation is added in the little petridish that diameter is 6cm (put a paper clip before the sterilization in the ware, be magnetic stirring apparatus down), petridish is placed under the ultra-violet lamp; Apart from 18cm, with gobo whole clean work station is covered, uncap then and under agitation condition, shine 15s, 30s respectively; 45s, 1min, 1min 15s; 1min 30s, the ware lid closes uv lamp again on the irradiation bonnet.Bacterium liquid after the mutagenesis more than dark condition held 20min, dilutes coating earlier again.Bacteria suspension is diluted to 10 by 10 times -5, 10 -6, from 10 -5With 10 -6In respectively take out 0.1mL join the screening culture medium flat board (peptone 10g/L, yeast extract 5g/L, NaCl g/L, sun blue 500mg/L, agar powder 18g/L pH7.0) goes up coating, leaves standstill after bacterium liquid infiltrates substratum and is inverted, in about 37 ℃ of constant temperature culture 12h.
The 3rd step, primary dcreening operation.After about 37 ℃ of constant temperature culture 12h; At first observe on blue flat board, the transparent circle size that periphery of bacterial colonies occurs, and measure its colony diameter and transparent circle diameter ratio (HC value); Select the big bacterium colony of its HC value; After under aseptic condition, its picking being cultivated 12h in the liquid LB substratum, using concentration is 20% glycerine-4 ℃ preservation, and treats liquid multiple sieve.
The 4th step, multiple sieve.After the bacterial strain activation culture that primary dcreening operation is obtained; On Bechtop, cultured bacterial classification is transferred to from test tube in the centrifuge tube of two bacterium of going out; After the balance on high-speed refrigerated centrifuge with 11000r/min, under 10 ℃ of environment centrifugal 2 minutes, abandon supernatant after the taking-up; Add an amount of sterilized water, mixing washing on oscillator.Leveling on electronic balance afterwards, repeated centrifugation operation once more.After washing 3 times, it is resuspended to add sterilized water, and cell density is 10 9, with the aseptic inoculation operating method, get 50 μ L and be inoculated in the 100mL sun blue liquid nutrient medium (prescription: the 250mg sun blue is dissolved in the 1000mL zero(ppm) water), stay the sun blue liquid nutrient medium of not inoculating bacterium liquid to be contrast, all be positioned over 37 ℃ of shaking tables and cultivate.After cultivating 12h, be reference with the sun blue liquid nutrient medium of not inoculating bacterium liquid, get the centrifugal back of 5mL bacterium liquid and survey the 545nm absorbance value, calculate percent of decolourization.Mutant strain ZN0871v11 has exceeded more than 80% than original starting strain percent of decolourization as a result, has reached 100%.
The percent of decolourization of dyestuff=(control group OD value-experimental group OD value)/control group OD * 100%
The 5th step; The pH damping fluid of different gradients or the metals ion of different concns are to the culture medium prescription of 7 kinds of dye decolored influential effects: earlier 7 kinds of dyestuffs of 500mg are dissolved in respectively in the 1000mL zero(ppm) water; Regulate dye solution with the pH damping fluid of different gradients or the metals ion of different concns then; Control 7 kinds of dyestuffs final concentration at 250mg/1000mL, Fe 2+, Cu 2+, Zn 2+, Ca 2+, Mg 2+, K +, Na +Final concentration be respectively 15,20mg/100mL.121 ℃ of high-temperature sterilization 30min.
In the 6th step, the decolouring rapidly and efficiently of dyeing waste water is handled.After mutant strain ZN0871v11 activation culture, with behind the cell washing 3 times, it is resuspended to add sterilized water in the above described manner, and cell density is 10 9Individual/mL, with the aseptic inoculation operating method, respectively get 100 μ L bacteria suspensions and be inoculated into respectively in all kinds of dye liquid substratum of 100mL, stay the corresponding dye liquid substratum of not inoculating bacterium liquid for contrast, place 5-10min behind the mixing.Subsequently, be reference with the corresponding dye liquid substratum of not inoculating bacterium liquid, get the centrifuged supernatant photometry absorption value of 5mL bacterium liquid, calculate percent of decolourization.The wavelength of photometry absorption value is respectively: sun blue is 545nm, and REACTIVE Yellow 160 is 415nm, and REACTIVE Yellow 145 is 419nm, and reactive orange 78 is 464nm, and HFGR REACTIVE Black HFGR RN-B is 571nm, and REACTIVE Red 195 is 510nm, and reactive yellow MS is 415nm.
Embodiment 2 handles the decolouring rapidly and efficiently of dyeing waste water under various conditions
In the present embodiment, according to the foregoing description 1 described method, verified that mutant strain ZN0871v11 cell is to the treatment effect of decolouring rapidly and efficiently of different dyes under conditions such as various pH, metals ion, temperature.Should be understood that the various conditions of the following stated only are exemplary, and should not be construed as limitation of the scope of the invention.
Embodiment 2-1
In pH5.0, temperature is 37 ℃, metal cations Fe 2+Final concentration is under the 20mg/100mL condition, adds in the mutant strain ZN0871v11 cell mixing 10 minutes, and mutant strain ZN0871v11 is 100% to the percent of decolourization of sun blue, records the COD that is caused by dyestuff and reduces to " 0 ".
Embodiment 2-2
At pH7.0, temperature is 25 ℃, Fe 2+Concentration be under the condition of 15mg/100mL, mutant strain ZN0871v11 is complete to the decolouring of sun blue dyestuff, in 5-10 minute, reaches maximum elimination factor, is 100% to the percent of decolourization of sun blue, and records the COD that is caused by dyestuff and reduce to " 0 ".
Embodiment 2-3
In pH5.0, temperature is 37 ℃, Cu 2+Concentration be under the condition of 15mg/100mL, mutant strain ZN0871v11 is thorough to the decolouring of sun blue dyestuff, in 5-10 minute, percent of decolourization reaches 100%, and records the COD that is caused by dyestuff and reduce to " 0 ".
Embodiment 2-4
In pH5.0, temperature is 37 ℃, Cu 2+Concentration be under the condition of 20mg/100mL, mutant strain ZN0871v11 is thorough to the decolouring of sun blue dyestuff, in 5-10 minute, percent of decolourization reaches 100%, and records the COD that is caused by dyestuff and reduce to " 0 ".
Embodiment 2-5
Under 37 ℃, 42 ℃ temperature, when not adding any ion, mutant strain ZN0871v11 can reach 100% to the percent of decolourization of sun blue when 37 ℃ and 42 ℃, and the decolorizing effect of dyestuff is consistent.Different temperature is less to the decolouring adsorption effect influence of thalline.The thermal adaptability that mutant strain ZN0871v11 is described is strong.
Embodiment 2-6
In pH value 5.0, when not adding any ion, add mutant strain ZN0871v11 cell mixing.Mutant strain ZN0871v11 has reached 92.8% to the percent of decolourization of sun blue dyestuff.
Embodiment 2-7
In pH5.0, temperature is 37 ℃, Zn 2+Concentration be under the condition of 20mg/100mL, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 89.73% to the percent of decolourization of sun blue dyestuff.
Embodiment 2-8
In pH5.0, temperature is 37 ℃, Ca 2+Concentration is under the condition of 20mg/100mL, adds in the mutant strain ZN0871v11 cell mixing 10 minutes, and mutant strain ZN0871v11 is 66.25% to the percent of decolourization of sun blue dyestuff.
Embodiment 2-9
In pH5.0, temperature is 37 ℃, metal cations Fe 2+When concentration is 20mg/100mL, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 100% to the percent of decolourization of REACTIVE Red 195.
Embodiment 2-10
In pH5.0, temperature is 37 ℃, metal cations Fe 2+When concentration is 20mg/100mL, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 69% to the percent of decolourization of reactive orange 78.
Embodiment 2-11
In pH5.0, temperature is 37 ℃, metal cations Fe 2+When concentration is 20mg/100mL, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 78.8% to the percent of decolourization of REACTIVE Yellow 145.
Embodiment 2-12
In pH5.0, temperature is 37 ℃, metal cations Fe 2+When concentration is 20mg/100mL, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 80.69% to the percent of decolourization of reactive yellow MS.
Embodiment 2-13
In pH5.0, temperature is 37 ℃, metal cations Fe 2+When concentration is 20mg/100mL, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 93.67% to the percent of decolourization of REACTIVE Yellow 160.
Embodiment 2-14
In pH6.0, temperature is 37 ℃, metal cations Fe 2+When concentration was 20mg/100mL, mutant strain ZN0871v11 was 96.2% for the percent of decolourization of HFGR REACTIVE Black HFGR KN-B.
Embodiment 2-15
At Cu 2+Concentration be under the condition of 20 ℃ of 20mg/100mL, pH5.0, room temperature, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 45.63% to the percent of decolourization of HFGR REACTIVE Black HFGR KN-B.
Embodiment 2-16
At Cu 2+Concentration be under the condition of 20 ℃ of 20mg/100mL, pH5.0, room temperature, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 59.27% to the percent of decolourization of REACTIVE Red 195.
Embodiment 2-17
At Cu 2+Concentration be under the condition of 20 ℃ of 20mg/100mL, pH5.0, room temperature, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 69% to the percent of decolourization of reactive orange 78.
Embodiment 2-18
At Cu 2+Concentration be under the condition of 20 ℃ of 20mg/100mL, pH5.0, room temperature, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 78.8% to the percent of decolourization of REACTIVE Yellow 145.
Embodiment 2-19
At Cu 2+Concentration be under the condition of 20 ℃ of 20mg/100mL, pH5.0, room temperature, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 80.69% to the percent of decolourization of reactive yellow MS.
Embodiment 2-20
At Cu 2+Concentration be under the condition of 20 ℃ of 20mg/100mL, pH5.0, room temperature, add in the mutant strain ZN0871v11 cell mixing 10 minutes, mutant strain ZN0871v11 is 62.24% to the percent of decolourization of REACTIVE Yellow 160.

Claims (2)

  1. Subtilis ( Bacillus subtilis) mutant strain ZN0871v11, its preserving number is CCTCC NO:M2011208.
  2. 2. the described bacillus subtilis mutant strain ZN0871v11 of claim 1 is in the dyeing waste water purposes in handling of decolouring fast.
    3. the described purposes of claim 2 is characterized in that, contains sun blue, REACTIVE Red 195, reactive orange 78, REACTIVE Yellow 145, reactive yellow MS, REACTIVE Yellow 160 and/or HFGR REACTIVE Black HFGR KN-B in the said dyeing waste water.
    4. claim 2 or 3 purposes is characterized in that in metal cations Fe 2+Existence under carry out said processing.
    5. the purposes of claim 4 is characterized in that metal cations Fe 2+Concentration is 20 mg/100 mL.
    6. claim 2 or 3 purposes is characterized in that at metals ion Cu 2+Existence under carry out said processing.
    7. the purposes of claim 6 is characterized in that metals ion Cu 2+Concentration is 20 mg/100 mL.
    8. claim 2 or 3 described purposes is characterized in that treatment temp is 20-42 ℃.
    9. claim 2 or 3 described purposes is characterized in that when not adding any ion, carrying out said processing at pH5.0.
    10. claim 2 or 3 described purposes is characterized in that adding Fe 2+Or Cu 2+Condition under be 5.0 or 7.0 to carry out said processing at pH.
CN201110173958A 2011-06-27 2011-06-27 Mutant strain ZN0871v11 and purpose thereof in rapid and efficient decolouring treatment of printing and dyeing waste water Active CN102352325B (en)

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PCT/CN2011/076880 WO2013000178A1 (en) 2011-06-27 2011-07-05 Bacillus subtilis mutant strain and use thereof in rapid and high efficiency decolorization treatment of dyeing wastewater

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CN103113481B (en) * 2012-08-16 2014-12-10 中南民族大学 Mutant strain ZN0871v11 cell wall peptidoglycan and preparation method thereof
CN103173382B (en) * 2012-12-28 2015-03-25 中南民族大学 Regeneration and organic substance recovery method of mutant strain ZN0871v11 after organic wastewater treatment
CN103910435B (en) * 2014-01-14 2015-09-30 暨南大学 The application of Methionin genus bacillus FS1 in dye degrades
CN103937697B (en) * 2014-01-14 2017-01-25 暨南大学 Bacterial strain for degrading dye with high efficiency
CN106830357A (en) * 2017-02-14 2017-06-13 厦门大学 A kind of method that Congo red waste water is processed with biological flocculant
CN107841478B (en) * 2017-12-18 2020-05-12 黄冈师范学院 Bacillus strain for efficient broad-spectrum decoloration and degradation of active dye and application thereof
CN110951641B (en) * 2019-12-11 2022-01-07 中国农业科学院植物保护研究所 Bacillus subtilis, microbial agent and application thereof
JP2022117758A (en) * 2021-02-01 2022-08-12 株式会社東芝 Wastewater treatment device and wastewater treatment method
CN117925475A (en) * 2024-01-30 2024-04-26 重庆港力环保股份有限公司 Bacillus clathratus capable of efficiently reducing chromaticity, breeding method thereof and compound microbial inoculum

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005013915A (en) * 2003-06-27 2005-01-20 Miki Riken Kogyo Kk Waste water treatment method
CN1234847C (en) * 2003-11-28 2006-01-04 广东省微生物研究所 Shewanella decolorationis
CN101463338B (en) * 2009-01-06 2010-09-01 东北农业大学 Bacillus subtilis and use thereof for degrading imazethapyr
CN101845411A (en) * 2010-03-25 2010-09-29 东华大学 Extraction method of reactive black 5 dye efficient decolorizing bacteria
CN101948778B (en) * 2010-08-30 2012-04-11 中国农业大学 Bacillus megaterium and application thereof to printing and dyeing wastewater treatment
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