CN102337212A - Pneumatic oscillatory type cell culture system - Google Patents
Pneumatic oscillatory type cell culture system Download PDFInfo
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- CN102337212A CN102337212A CN2011102970282A CN201110297028A CN102337212A CN 102337212 A CN102337212 A CN 102337212A CN 2011102970282 A CN2011102970282 A CN 2011102970282A CN 201110297028 A CN201110297028 A CN 201110297028A CN 102337212 A CN102337212 A CN 102337212A
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- bottle
- cell culture
- water bath
- oscillatory type
- type cell
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The invention discloses a pneumatic oscillatory type cell culture system which comprises a constant temperature water bath box, wherein a culture bottle is arranged in the constant temperature water bath box and the culture bottle is internally provided with an air pump device. According to the invention, since the air pump device is arranged in the culture bottle, after the culture bottle is full filled with culture liquid containing experiment cells, the constant temperature water bath box can provide constant temperature water bath, the air pump device can pump air in the culture liquid, the pumped air naturally drives the culture liquid to turn over and oscillate, and because the oscillation is not forced oscillation caused by a mechanical stirring device, the cells can not be damaged. The pneumatic oscillatory type cell culture system has the advantages of simple and ingenious structure and good oscillation effect, can be widely applied to experiments of cell culture, fermentation, hybridization, biochemistry, enzyme and cell tissue and the like.
Description
Technical field
The present invention relates to a kind of experimental system of using when carrying out Bioexperiment, particularly a kind of cell culture system.
Background technology
When carrying out experiment such as cell cultures, fermentation, hybridization, biological chemistry and enzyme and cell tissue; Usually need to build a cell culture environment and come out, and have the microorganism cells or the bacterium colony of particular requirement for some, they need be survived in nutrient solution and breeding; And according to requirement of experiment; Nutrient solution must maintain in certain temperature, and will vibrate, and lets experimental subjectss such as cell in nutrient solution, be kept in motion.Therefore, the cell culture system with temperature control and oscillation functions is absolutely necessary for these experiments.But present cell culture system can only be provided the heat that needs at the most by the thermostat container of outside; But the vibration of nutrient solution can only depend on mechanical oscillation, just utilizes the external means vibration, rocks the culturing bottle of culture systems, or directly whipping appts is put in the culturing bottle; Be inserted into and carry out mechanical stirring in the nutrient solution; And in fact, a little less than these mode of vibrations or the vibration too, can not reach requirement of experiment; It is excessive to vibrate, and the cell tissue in the nutrient solution is damaged.
Summary of the invention
The object of the invention is for a kind of pneumatic oscillatory type cell culture system is provided.
The solution that the present invention solves its technical problem is:
Pneumatic oscillatory type cell culture system, it comprises constant water bath box, is provided with culturing bottle in the said constant water bath box, is provided with pump device in the said culturing bottle.
As the further improvement of technique scheme, said pump device comprises and is located at the bottle stopper culturing bottle bottleneck, that have venting port, also is installed with the tracheae that space in the bottle of culturing bottle is in communication with the outside in the bottle stopper, and tracheae bottle outside is communicated with the mixing gas cylinder.
As the further improvement of technique scheme, said tracheae is provided with pressure regulator valve with the connecting pipe that mixes gas cylinder.
As the further improvement of technique scheme, the end of said tracheae end in the culturing bottle bottle goes out to be connected with many pores sand ball.
As the further improvement of technique scheme, the top of said constant water bath box is provided with filtering layer.
The invention has the beneficial effects as follows: the present invention is through being provided with pump device in culturing bottle; Like this after the culturing bottle Sheng has been carried the nutrient solution that contains experimental cell; Constant water bath box can provide water bath with thermostatic control, pump device can be in nutrient solution pump gas, the gas that pumps drives nutrient solution naturally and rolls, vibrates; And therefore this vibration can not damage by pair cell owing to be not the forced vibration that mechanical stirring device brings.Simple and ingenious structure of the present invention, oscillation effect is good, can be widely used in carrying out in the middle of the experiment such as cell cultures, fermentation, hybridization, biological chemistry and enzyme and cell tissue.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is further described.
Fig. 1 is the one-piece construction synoptic diagram of the present invention when working.
Embodiment
With reference to Fig. 1, pneumatic oscillatory type cell culture system, it comprises constant water bath box 3, is provided with culturing bottle 2 in the said constant water bath box 3, is provided with pump device in the said culturing bottle 2.Wherein constant water bath box 3 is existing conventional Bioexperiment water bath apparatus, has water circulation system and heating system, can carry out circulator bath in the outside of culturing bottle 2, keeps the nutrient solution in the culturing bottle 2 to be in the experimental temperature that needs.Pump device, as its name suggests, it has the function of pump gas; Just form bubble toward the interior ventilation of liquid; The liquid vibration of can rolling naturally when bubble rises is so when culturing bottle 2 is provided with pump device after, the nutrient solution that a needs will contain the experimental cell sample is injected in the culturing bottle 2 and is full of; The ejector priming device of air can reach oscillation effect.
Further as preferred embodiment; Said pump device comprises and is located at bottle stopper culturing bottle 2 bottlenecks, that have venting port; Also be installed with in the bottle stopper the tracheae 7 that is in communication with the outside of space in the bottle of culturing bottle 2, tracheae 7 bottle outside is communicated with mixing gas cylinder 4.Venting port is that culturing bottle 2 internal gas pressures are excessive and be provided with when preventing pump gas, and gas feeds a nutrient solution in the bottle from mixing gas cylinder 4 outputs from tracheae 7, forms to emit on the bubble, discharges from venting port then.
Further as preferred embodiment, said tracheae 7 is provided with pressure regulator valve 5 with the connecting pipe that mixes gas cylinder 4.Pressure regulator valve 5 is used to regulate the pressure that inputs to the gas in the culturing bottle 2.
Further as preferred embodiment, the end of said tracheae 7 end in 2 bottles on culturing bottle goes out to be connected with many pores sand ball 6.Particularly; That said many pores sand ball 6 can adopt is existing, in the view fish culture used oxygen supply sand ball, it is a loose and porous structure, when mixed gas feeds at the most pore sand ball 6 from tracheae 7 after; Gas dispersion is that a plurality of micro-bubbles are discharged; Drain in the nutrient solution,, therefore can further optimize the oscillation effect of liquid because bubbles volume is many, bubble is little.
Further as preferred embodiment, the top of said constant water bath box 3 is provided with filtering layer 1.Particularly, can on the architecture basics of original water bath, improve, add filtering layer at an upper portion thereof; Said filtering layer 1 material therefor is a ultra-filtration membrane; Its filtering accuracy is 0.01 micron, can filter out the various bacteria that comprises intestinal bacteria, reaches the clean function of operation.
Workflow of the present invention is described below: a culturing bottle 2 of having put cell mikrobe and nutrient solution in advance is placed in the constant water bath box 3, lets culturing bottle 2 major parts be immersed in the thermostatical circulating water stream once; Start constant water bath box 3, let culturing bottle 2 be in the circulator bath; Open pressure regulator valve 5 and regulate air pressure, let mixed gas, slowly pass in the culturing bottle 2 through tracheae 7, and from many pores sand ball 6, discharge, become a large amount of micro-bubbles during discharge, nutrient solution is vibrated from mixing gas cylinder 4 outputs.
Through such measure, can guarantee effectively that cell keeps ortho states in cultivation, do not smashed by external force, reach better cultivation effect; Obnoxious flavour that produces in the cell cultivation process simultaneously and mikrobe, dust can pass through filtering layer 1 high-efficiency filtering and purifying, thereby play the effect of protection environment.This system cost is cheap, and is easy to use, and current consumption is low, has excellent popularization to be worth.
More than be that preferred embodiments of the present invention is specified; But the invention is not limited to said embodiment; Those of ordinary skill in the art also can make all equivalent variations or replacement under the prerequisite of spirit of the present invention, modification that these are equal to or replacement all are included in the application's claim institute restricted portion.
Claims (5)
1. pneumatic oscillatory type cell culture system is characterized in that: it comprises constant water bath box (3), is provided with culturing bottle (2) in the said constant water bath box (3), and said culturing bottle is provided with pump device in (2).
2. pneumatic oscillatory type cell culture system according to claim 1; It is characterized in that: said pump device comprises and is located at culturing bottle (2) bottle stopper bottleneck, that have venting port; Also be installed with in the bottle stopper the tracheae (7) that is in communication with the outside of space in the bottle of culturing bottle (2), tracheae (7) bottle outside is communicated with mixing gas cylinder (4).
3. pneumatic oscillatory type cell culture system according to claim 2 is characterized in that: said tracheae (7) is provided with pressure regulator valve (5) with the connecting pipe that mixes gas cylinder (4).
4. according to claim 2 or 3 described pneumatic oscillatory type cell culture systems, it is characterized in that: the end of said tracheae (7) end in culturing bottle (2) bottle goes out to be connected with many pores sand ball (6).
5. pneumatic oscillatory type cell culture system according to claim 4 is characterized in that: the top of said constant water bath box (3) is provided with filtering layer (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110297028 CN102337212B (en) | 2011-09-30 | 2011-09-30 | Pneumatic oscillatory type cell culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110297028 CN102337212B (en) | 2011-09-30 | 2011-09-30 | Pneumatic oscillatory type cell culture system |
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| Publication Number | Publication Date |
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| CN102337212A true CN102337212A (en) | 2012-02-01 |
| CN102337212B CN102337212B (en) | 2013-09-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201110297028 Expired - Fee Related CN102337212B (en) | 2011-09-30 | 2011-09-30 | Pneumatic oscillatory type cell culture system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108467834A (en) * | 2018-02-11 | 2018-08-31 | 温州医科大学 | A kind of small-sized cell culture unit of pressure controllable |
| US11624049B2 (en) | 2017-12-22 | 2023-04-11 | Orizuru Therapeutics, Inc. | Cell culturing apparatus, culture solution aspirator, and cell culturing method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1279291A (en) * | 2000-06-06 | 2001-01-10 | 华东理工大学 | Process and reactor for preparing ginseng saponin and polyose from notoginseng cells |
| CN1817108A (en) * | 2006-03-07 | 2006-08-16 | 天津大学 | Airlift biological reactor for culturing non-fixed roots of Danshen |
| CN1902304A (en) * | 2003-11-18 | 2007-01-24 | 雀巢技术公司 | Cell culture system |
| CN201545840U (en) * | 2009-09-21 | 2010-08-11 | 惠识瑶 | Packed bed cell bioreactor of stirring-free device |
| CN201581081U (en) * | 2009-11-03 | 2010-09-15 | 金宇保灵生物药品有限公司 | Cell culture reactor |
| CN101899392A (en) * | 2010-07-12 | 2010-12-01 | 浙江省林业科学研究院 | Internal circulation gas-lift type cell culture apparatus |
| CN102021115A (en) * | 2009-09-21 | 2011-04-20 | 惠识瑶 | Packed bed type cell bioreactor without stirrer and method for cultivating animal cells |
-
2011
- 2011-09-30 CN CN 201110297028 patent/CN102337212B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1279291A (en) * | 2000-06-06 | 2001-01-10 | 华东理工大学 | Process and reactor for preparing ginseng saponin and polyose from notoginseng cells |
| CN1902304A (en) * | 2003-11-18 | 2007-01-24 | 雀巢技术公司 | Cell culture system |
| CN1817108A (en) * | 2006-03-07 | 2006-08-16 | 天津大学 | Airlift biological reactor for culturing non-fixed roots of Danshen |
| CN201545840U (en) * | 2009-09-21 | 2010-08-11 | 惠识瑶 | Packed bed cell bioreactor of stirring-free device |
| CN102021115A (en) * | 2009-09-21 | 2011-04-20 | 惠识瑶 | Packed bed type cell bioreactor without stirrer and method for cultivating animal cells |
| CN201581081U (en) * | 2009-11-03 | 2010-09-15 | 金宇保灵生物药品有限公司 | Cell culture reactor |
| CN101899392A (en) * | 2010-07-12 | 2010-12-01 | 浙江省林业科学研究院 | Internal circulation gas-lift type cell culture apparatus |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11624049B2 (en) | 2017-12-22 | 2023-04-11 | Orizuru Therapeutics, Inc. | Cell culturing apparatus, culture solution aspirator, and cell culturing method |
| CN108467834A (en) * | 2018-02-11 | 2018-08-31 | 温州医科大学 | A kind of small-sized cell culture unit of pressure controllable |
| CN108467834B (en) * | 2018-02-11 | 2023-07-25 | 温州医科大学 | Pressure-controllable small cell culture device |
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| CN102337212B (en) | 2013-09-11 |
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Effective date of registration: 20170424 Address after: Tiantai Whampoa District of Guangzhou City, Guangdong province 510000 Road No. 1 Room 201 Patentee after: Guangzhou Yoder Biological Technology Co., Ltd. Address before: 510643 Guangzhou, Baiyun District, Beijing, Fujian Province, Chunlan street, No. 804, room 1, No. two Patentee before: Wu Xiaochun |
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Granted publication date: 20130911 Termination date: 20180930 |
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