CN102329851B - Sulfate reduction bacteria culture medium for oil field sewage treatment system - Google Patents
Sulfate reduction bacteria culture medium for oil field sewage treatment system Download PDFInfo
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- CN102329851B CN102329851B CN201110295193A CN201110295193A CN102329851B CN 102329851 B CN102329851 B CN 102329851B CN 201110295193 A CN201110295193 A CN 201110295193A CN 201110295193 A CN201110295193 A CN 201110295193A CN 102329851 B CN102329851 B CN 102329851B
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Abstract
The invention relates to a sulfate reduction bacteria culture medium for an oil field sewage treatment system. Oil field sewage contains a certain quantity of bacteria which can generate a certain corrosion effect on equipment and a line pipe, thus the oil field sewage is necessarily sterilized before reinjection is carried out, so that the content of the bacteria reaches the water reinjection requirement. The culture medium provided by the invention comprises the following components in parts by weight: 0.09-0.10 part of potassium dihydrogenphosphate, 0.029-0.03 part of ammonium chloride, 0.19-0.20 part of magnesium sulfate heptahydrate, 0.083-0.085 part of anhydrous sodium sulfate, 0.39-0.40 part of sodium lactate, 0.039-0.04 part of vitamin c, 0.14-0.15 part of yeast extract powder, 0.98-1.05 parts of sodium chloride, 0.0029-0.003 part of L-cysteine hydrochloride, 0.015-0.020 part of calcium carbonate, 0.014-0.15 part of 10% sodium hydroxide, 10 parts of iron and 97.84-97.86 parts of water. The culture medium has the advantages of high sensitivity, high accuracy and short detection period on test of sulfate reduction bacteria, and is convenient for quick detection of the bacteria in an oil field.
Description
Technical field
The present invention relates to a kind of sulfate reduction bacterium culture medium that is used for the disposing polluted water in oil system.
Background technology
In the oilfield process,, adopt the method for water flooding recovery for improving oil recovery factor more; For practicing thrift earth Freshwater resources, re-injection water is many deviates from water from crude oil, i.e. oilfield sewage; The bacterium that contains some amount in the oilfield sewage; Equipment and pipeline are produced certain corrosion,, make bacteria content reach the requirement of re-injection water so oilfield sewage must carry out germicidal treatment before re-injection.It is a kind of effective germicidal treatment mode that oilfield sewage is added sterilant, and the detection of bacteria content all will be used bacteria culture medium in the sterilization effect of sterilant and the return water thereof.
Summary of the invention
It is high that the technical problem that the present invention solved provides a kind of measurement sensitivity height, accuracy to sulphate reducing bacteria, the sense cycle shortening, and convenience is to the sulfate reduction bacterium culture medium that is used for the disposing polluted water in oil system of the rapid detection of bacterium in the oilfield sewage.
For solving above-mentioned technical problem, the technical scheme that the present invention takes:
A kind of sulfate reduction bacterium culture medium that is used for the disposing polluted water in oil system, its special character is: be made up of according to weight part following material:
Potassium hydrogenphosphate: 0.09-0.10 part, ammonium chloride: 0.029-0.03 part, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.19-0.20 part; SODIUM SULPHATE ANHYDROUS 99PCT: 0.083-0.085 part, Sodium.alpha.-hydroxypropionate: 0.39-0.40 part, vitamin c: 0.039-0.04 part; Yeast soaks powder: 0.14-0.15 part, and sodium-chlor: 0.98-1.05 parts, L-cysteine hydrochloride: 0.0029-0.003 part; Lime carbonate: 0.015～0.020 part; The sodium hydroxide of concentration 10%: 0.014-0.15 part, iron: 10 parts, deionized water: 97.84-97.86 parts.
The above-mentioned sulfate reduction bacterium culture medium that is used for the disposing polluted water in oil system, its special character is: be made up of according to weight part following material:
Potassium hydrogenphosphate: 0.095 part, ammonium chloride: 0.0295 part, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.195 part, SODIUM SULPHATE ANHYDROUS 99PCT: 0.084 part; Sodium.alpha.-hydroxypropionate: 0.395 part, vitamin c: 0.0395 part, yeast soaks powder: 0.145 part; Sodium-chlor: 1.015 parts, the L-cysteine hydrochloride: 0.00295 part, lime carbonate: 0.0175 part; The sodium hydroxide of concentration 10%: 0.082 part, iron: 10 parts, deionized water: 97.85 parts.
Compared with prior art, the present invention is high to the measurement sensitivity of sulphate reducing bacteria, accuracy is high, and sense cycle shortens, convenient rapid detection to bacterium in the oilfield sewage.
Embodiment
Below in conjunction with embodiment the present invention is elaborated.
The present invention is made up of according to weight part following material:
Potassium hydrogenphosphate: 0.09-0.10 part, ammonium chloride: 0.029-0.03 part, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.19-0.20 part; SODIUM SULPHATE ANHYDROUS 99PCT: 0.083-0.085 part, Sodium.alpha.-hydroxypropionate: 0.39-0.40 part, vitamin c: 0.039-0.04 part; Yeast soaks powder: 0.14-0.15 part, and sodium-chlor: 0.98-1.05 parts, L-cysteine hydrochloride: 0.0029-0.003 part; Lime carbonate: 0.015～0.020 part; The sodium hydroxide of concentration 10%: 0.014-0.15 part, iron: 10 parts, deionized water: 97.84-97.86 parts.
The present invention is made up of according to weight part following material:
Potassium hydrogenphosphate: 0.095 part, ammonium chloride: 0.0295 part, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.195 part, SODIUM SULPHATE ANHYDROUS 99PCT: 0.084 part; Sodium.alpha.-hydroxypropionate: 0.395 part, vitamin c: 0.0395 part, yeast soaks powder: 0.145 part; Sodium-chlor: 1.015 parts, the L-cysteine hydrochloride: 0.00295 part, lime carbonate: 0.0175 part; The sodium hydroxide of concentration 10%: 0.082 part, iron: 10 parts, deionized water: 97.85 parts.
Embodiment 1:
The present invention is made up of according to weight part following material:
Potassium hydrogenphosphate: 0.09 part, ammonium chloride: 0.029 part, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.19 part, SODIUM SULPHATE ANHYDROUS 99PCT: 0.083 part; Sodium.alpha.-hydroxypropionate: 0.39 part, vitamin c: 0.039 part, yeast soaks powder: 0.14 part; Sodium-chlor: 0.98 part, the L-cysteine hydrochloride: 0.0029 part, lime carbonate: 0.015 part; The sodium hydroxide of concentration 10%: 0.014 part, iron: 10 parts, deionized water: 97.84 parts.
After said ratio, prepare through following steps:
(1), preparation
A, be ready to clean exsiccant glassware and used reagent, reagent must meet the requirement of biological reagent and before the deadline;
B, according to the consumption of substratum preparation total amount and each reagent of formula calculation, accurately measure 1000mL zero(ppm) water with graduated cylinder, service precision is 0.0001 part a electronic analytical balance in the weighing process;
C, by the sequencing of reagent in the prescription with the accurate weighing all ingredients of electronic analytical balance; An amount of zero(ppm) water dissolves fully in the adding 1000mL graduated cylinder in the 100mL small beaker; Change in the 1000mL beaker,, washing fluid is changed in the 1000mL beaker each small beaker flushing 3～5 times;
D, treat in the 1000mL beaker that all reagent dissolve fully after, add in the graduated cylinder remaining zero(ppm) water to large beaker;
E, the culture medium solution that sucks a small amount of mixing with valinche drop on the Accurate pH value test paper, measure the pH value of nutrient solution, the NaOH solution adjusting pH value with 10%.Dropwise add 10% NaOH solution in the regulate process, the limit edged stirs, and prevents the too high destruction nutrition of local basicity based component, and tests with the pH test paper frequently, till the pH value is 8.0;
(2), the packing of substratum and sterilization
A, sulfate reduction bacterium culture medium should accurately move into 9mL sulphate reducing bacteria culture medium solution to the 10mL vial before packing, the little iron nail that will handle well with absolute ethyl alcohol and filter paper is in advance again put into vial, adds a cover then and seals;
Autoclave sterilizer is put in b, the sulfate reduction bacterium culture medium test bottle layering that above-mentioned branch is installed, bottle with bottle between should leave the space, capping; It is 126 ℃ down about sterilization 30min that pressure is set in 0.145MPa, temperature; Sterilization finishes its automatic back pressure of relief to normal pressure, opens purging valve and uncaps, and is cooled to normal temperature; Take out bacteria culture bottle, for use after the assay was approved.
Compared with prior art, beneficial effect of the present invention:
(1) the sulfate reduction bacterium culture medium is highly sensitive to the identification of sulphate reducing bacteria, and the substratum colour-change is obvious, is easy in the testing process observe.
(2) the sulfate reduction bacterium culture medium is high to the recognition accuracy of sulphate reducing bacteria, and the difference in the testing process between the parallel appearance is little.
(3) the sulfate reduction bacterium culture medium is short to the recognition time of sulphate reducing bacteria, and final recognition time is 7～14 days, has shortened 7 days than in the market like product time.
Embodiment 2:
The present invention is made up of according to weight part following material:
Potassium hydrogenphosphate: 0.095 part, ammonium chloride: 0.0295 part, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.195 part, SODIUM SULPHATE ANHYDROUS 99PCT: 0.084 part; Sodium.alpha.-hydroxypropionate: 0.395 part, vitamin c: 0.0395 part, yeast soaks powder: 0.145 part; Sodium-chlor: 1.015 parts, the L-cysteine hydrochloride: 0.00295 part, lime carbonate: 0.0175 part; The sodium hydroxide of concentration 10%: 0.082 part, iron: 10 parts, deionized water: 97.85 parts.
The preparation method is with embodiment 1.
Embodiment 3:
The present invention is made up of according to weight part following material:
Potassium hydrogenphosphate: 0.10 part, ammonium chloride: 0.03 part, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.20 part, SODIUM SULPHATE ANHYDROUS 99PCT: 0.085 part; Sodium.alpha.-hydroxypropionate: 0.40 part, vitamin c: 0.04 part, yeast soaks powder: 0.15 part; Sodium-chlor: 1.05 parts, the L-cysteine hydrochloride: 0.003 part, lime carbonate: 0.020 part; The sodium hydroxide of concentration 10%: 0.15 part, iron: 10 parts, deionized water: 97.86 parts.
The preparation method is with embodiment 1.
The substratum that employing embodiment 2 makes is according to " SY/T 0532-93 oilfield injection water bacteria analyzing method-disappearance dilution method "; Respectively recover the oil two factories, three factories of recovering the oil, the several on-the-spot water sulfate reduction bacterial contents that inject of four factories of recovering the oil of long celebrating oil field are analyzed, the result is following:
Claims (1)
1. sulfate reduction bacterium culture medium that is used for the disposing polluted water in oil system is characterized in that: be made up of according to weight part following material:
Potassium hydrogenphosphate: 0.095 part, ammonium chloride: 0.0295 part, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5: 0.195 part, SODIUM SULPHATE ANHYDROUS 99PCT: 0.084 part; Sodium.alpha.-hydroxypropionate: 0.395 part, vitamin c: 0.0395 part, yeast soaks powder: 0.145 part; Sodium-chlor: 1.015 parts, the L-cysteine hydrochloride: 0.00295 part, lime carbonate: 0.0175 part; The sodium hydroxide of concentration 10%: 0.082 part, iron: 10 parts, deionized water: 97.85 parts.
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| CN201110295193A CN102329851B (en) | 2011-10-08 | 2011-10-08 | Sulfate reduction bacteria culture medium for oil field sewage treatment system |
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| CN201110295193A CN102329851B (en) | 2011-10-08 | 2011-10-08 | Sulfate reduction bacteria culture medium for oil field sewage treatment system |
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| CN102329851B true CN102329851B (en) | 2012-09-26 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3183332A1 (en) | 2014-08-20 | 2017-06-28 | 3M Innovative Properties Company | Self-contained anaerobic culture device for sulfate-reducing microorganisms |
| US11702620B2 (en) | 2015-04-29 | 2023-07-18 | 3M Innovative Properties Company | Self-contained anaerobic environment-generating culture device |
| EP3356510B1 (en) | 2015-09-28 | 2021-10-27 | 3M Innovative Properties Company | Self-contained anaerobic culture device with microcompartments |
| CN107216998B (en) * | 2016-03-22 | 2020-05-19 | 中国石油化工股份有限公司 | Automatic device for rapidly measuring bacterial content in oil field sewage and finished oil |
| CN107937272B (en) * | 2017-12-31 | 2021-03-26 | 中国科学院沈阳应用生态研究所 | Culture medium for hydrogen sulfide producing bacteria in oil field and application thereof |
| CN111433371B (en) * | 2018-08-08 | 2021-12-28 | 北京华油科隆开发公司 | Desulfurization vibrio bacteria test composition and preparation method and application thereof |
| CN111393045B (en) * | 2020-03-27 | 2022-12-30 | 中核第七研究设计院有限公司 | Method for preparing cementing material from waste incineration fly ash |
| CN113881594B (en) * | 2021-10-08 | 2023-05-30 | 辽宁大学 | Optimized sulfate reducing bacteria culture medium and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2004048607A1 (en) * | 2002-11-22 | 2004-06-10 | Enitecnologie S.P.A. | Method for the identification of sulfo-reducing bacteria |
| CN1696306A (en) * | 2005-05-16 | 2005-11-16 | 哈尔滨工业大学 | Method for quantitative detecting water quality in oil field through sulfate reducting bacteria |
| CN101029328A (en) * | 2006-11-30 | 2007-09-05 | 白莉 | Sulfate-reducing bacteria testing fluid and testing bottle |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004048607A1 (en) * | 2002-11-22 | 2004-06-10 | Enitecnologie S.P.A. | Method for the identification of sulfo-reducing bacteria |
| CN1696306A (en) * | 2005-05-16 | 2005-11-16 | 哈尔滨工业大学 | Method for quantitative detecting water quality in oil field through sulfate reducting bacteria |
| CN101029328A (en) * | 2006-11-30 | 2007-09-05 | 白莉 | Sulfate-reducing bacteria testing fluid and testing bottle |
Non-Patent Citations (1)
| Title |
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| 崔心水等.硫酸盐还原菌培养基组分及培养条件的优化.《西安工程大学学报》.2009,第23卷(第2期),全文. * |
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