CN102327185A - Method and device for loading substance with porous bracket - Google Patents
Method and device for loading substance with porous bracket Download PDFInfo
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- CN102327185A CN102327185A CN201110196769A CN201110196769A CN102327185A CN 102327185 A CN102327185 A CN 102327185A CN 201110196769 A CN201110196769 A CN 201110196769A CN 201110196769 A CN201110196769 A CN 201110196769A CN 102327185 A CN102327185 A CN 102327185A
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- porous support
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Abstract
The invention belongs to the technical field of material science and biomedical engineering, and particularly relates to a method and a device for loading a substance with a porous bracket. The porous bracket is mixed with the substance to be loaded and is placed under negative pressure during continuous oscillation, so that the substance to be loaded or solution of the substance to be loaded is filled into holes in the bracket, and thus the purpose of loading the solution of the substance to be loaded or the substance to be loaded in the bracket via absorption can be achieved. The device comprises a container with the porous bracket and the solution of the substance to be loaded, an oscillating device and a vacuum device, one end of the container is closed, the other end of the container is open, the closed end of the container is arranged in the oscillating device, and the open end of the container is connected with the vacuum device in a sealing way. The method is simple, and the device is convenient to assembly and is particularly suitable for drug loading of hydrophobic porous brackets.
Description
Technical field
The invention belongs to material science and biomedical engineering technology field, be specifically related to a kind of method and apparatus of porous support load material.
Background technology
Porous support has important use in fields such as organizational projects.Except porous support materials and the seed cell, somatomedin is also very important in the Tissue Engineering Study.Somatomedin is generally reactive protein, and is not only very valuable, and directly can cause quick loss, so (as: being adsorbed in the inner surface of porous support) is just necessary in the middle of in advance somatomedin being carried on support.But many porous supports are hydrophobic, and the aqueous solution of somatomedin is not easy to get into the inside in hole, and are therefore, how easy, just become a technical problem in the middle of somatomedin is loaded on porous support effectively.In like manner, carrying medicament also can run into such problem in the middle of porous support if desired.
At present, much having good biocompatibility and biodegradable hydrophobic polymer has also obtained in medicine sustained release field using widely.As the carrier material of medicine, people make such polymer carrier formats such as capsule, microsphere and contain the medicine carrying thing.How there is technical difficulty in medicine carrying always with this porous hydrophobic polymer, because polymer itself is difficult to by water-wet, and most medicine all is an aqueous solution, so up to the present, does not also see a suitable method and is used for porous hydrophobic polymer medicine carrying.
It is a kind of easy to operate that the present invention provides, and is applicable to the method and apparatus of porous hydrophobic polymer medicine carrying.The present invention adopts the mode of negative pressure+vortex to make liquid charge into internal stent more simply and realizes support load material.
The method of the invention and device are not limited to medicine carrying and somatomedin, also are not limited to hydrophobicity support and water-soluble substances, are suitable for too for other situation.
Summary of the invention
The object of the present invention is to provide a kind of easy to operate, the widely applicable method and apparatus that is used for porous support load material.
The method that is used for porous support load material provided by the invention specifically is with porous support and by the material of load, or is mixed by the solution of load material; Constantly placing under the negative pressure in the vibration then, making loaded article or loaded article solution be full of the hole of internal stent, making the load material be carried in the middle of the support through modes such as absorption; Dry support promptly obtains the porous support of load material.
Among the present invention; The host material of said porous support; Any in the following macromolecular material: poly DL-lactide, gather the L-lactide, gather Acetic acid, hydroxy-, bimol. cyclic ester, the copolymer of polylactic acid-glycolic guanidine-acetic acid, poly 3-hydroxy butyrate, polyhydroxyalkanoate, poly-epsilon-caprolactone, gather ε-alkyl and replace caprolactone, gather δ-Wu Neizhi, Merlon, poe, polymethyl methacrylate, gather, gather dioxane, polyether ester to dioxanone; Polystyrene, polrvinyl chloride, polyacrylate, polymethacrylates, gathering carbonic acid vinegar, nylon, polyurethane, polyformaldehyde, polyvinyl alcohol, polyvinyl acetate, polysiloxanes, perhaps is any in any type of copolymer or the blend of above-mentioned each kind polyester.
Among the present invention; The host material of said porous support; Also can be Inorganic Non-metallic Materials such as pottery, glass, carbon element; Comprise titanium oxide, aluminium oxide, calcium oxide, sodium oxide, silicon oxide, calcium phosphate series (hydroxyapatite, tricalcium phosphate, tetracalcium phosphate, calcium pyrophosphate, biphasic calcium phosphate, calcium polyphosphate), calcium sulfate, calcium carbonate or Low Temperature Isotropic Carbon, perhaps be above mixtures of material or with the mixture of additive.
Among the present invention, the host material of said porous support also can be the complex of above-mentioned certain macromolecular material and Inorganic Non-metallic Materials.
Among the present invention, the size in the hole of said porous support is 1nm-2000 μ m, is preferably 1-600 μ m.
Among the present invention, described loaded article is certain medicine, is specially to dissolve in the solvent or finely disseminated material, perhaps a material as liquid condition in solvent.Comprise some water solublity or finely disseminated material in water; For example plain type of cephalo, penicillin medicine (like cefoperazone sodium, cefradine, cefotaxime sodium, cefazolin sodium, penicillin sodium, benzylpenicillin potassium etc.); Quinolones hydrochlorate or acylate (example hydrochloric acid ofloxacin, hydrochloric acid norfloxacin etc.); Clindamycin hydrochloride or other salt also have water-soluble pesticides such as azithromycin, Roxithromycin, paclitaxel, famotidine, oleanolic acid.Described loaded article can also be the combination in any of water-soluble chitosan, hydroxyapatite, tricalcium phosphate and derivant thereof and above-mentioned various materials for polypeptides matters such as casein, gelatin, electrophoresis gamma Globulin, human albumin (HAS), Sanguis Bovis seu Bubali albumin protein such as (BSA) and various somatomedin also.
Among the present invention, said negative pressure can adopt vacuum oil pump, vacuum pump equal vacuum device to produce.The vacuum pressure scope is 0～-0.1MPa.Pressure 1min-24h action time, the preferential interaction time is 5min-5h.Can be uninterrupted pressurization, also can be for being interrupted pressurization.
Among the present invention, described vibration can be produced by vibrations such as vortex vibrating machine, cyclotron oscillation machine, disk vibration machine, vibrating device.Vibration can also can be intermittent oscillation for continuous oscillation, and vibration total time is 1S-24h.Preferably use duration of oscillation to be 1min-1h.
Among the present invention, the method for said dry support can be used drying modes such as lyophilization, vacuum drying.Drying time 1-96h.Preferentially selecting drying time is 6-48h.
The device that is used for the inventive method comprises: be loaded with porous support and by the load material or by the container of load substance solution, vibrating device, vacuum equipment; The sealing of container one end; One end opening, blind end places vibrating device, and opening links to each other with the vacuum equipment sealing.
In apparatus of the present invention, the material of said container can be bottle or the pipe that one or more materials of glass, pottery, macromolecular material, metal material are processed.
In apparatus of the present invention, the opening of said container links to each other through joint sealing with vacuum equipment, and joint can be for the vacuum adapter of band piston, not with the joint of piston etc.
The present invention has following advantage:
1. the method for the porous support load material that proposes of the present invention, technology is simple, is particularly suitable for the hole wall absorption medicine of hydrophobic porous support.
2. the device of the porous support load material that proposes of the present invention, simple in structure, overlap joint is convenient, the device materials range of choice is wide, quick detachable.
3. the device of the porous support load material of the present invention's proposition can change according to experiment condition and contain the container that carries porous support and loaded article, realize a plurality of support load materials simultaneously.
4. the method for the porous support load material of the present invention's proposition also can be used to the inner medicine of FirebirdTM.
Description of drawings
Fig. 1 is a porous support load material device sketch map.
Label among the figure: 1 is porous support, and 2 is drug solution, and 3 for being with ground-in glass tubing, and 4 is vacuum adapter, and 5 for connecting vacuum pump, and 6 are vortex vibration pallet.
The specific embodiment
Through embodiment the present invention is further explained below.
Porous polylactic-co-glycolic acid support (size in hole is 180-280 μ m) and bovine serum albumin (BSA) aqueous solution are placed the glass reaction tube of 10ml, and the reaction tube opening links to each other with vacuum pump through vacuum adapter.The other end is positioned in the vibration pallet of vortex agitator.Open vacuum pump and vortex agitator, vacuum pressure-0.09MPa, vibration 30min removes negative pressure of vacuum, and the failure of oscillations is taken out the polylactic-co-glycolic acid support, dry 24h.
Porous polylactic-co-glycolic acid copolymer support (size in hole is 10-50 μ m) and BSA aqueous solution are placed the pyriform reaction vessel, and the reaction vessel opening links to each other with vacuum pump through the vacuum adapter of band piston.The other end is positioned in the vibration pallet of vortex agitator.Open vacuum pump and vortex agitator, vacuum pressure-0.09MPa, vibration 15min removes negative pressure of vacuum; The failure of oscillations connects negative pressure more again, and the 15min that then vibrates removes negative pressure then; The failure of oscillations is taken out polylactic-co-glycolic acid copolymer support, dry 24h.
Porous polylactic acid bracket (size in hole is 50-150 μ m) and paclitaxel aqueous solution are placed the pyriform reaction vessel, and the reaction vessel opening links to each other with vacuum pump through the vacuum adapter of band piston.The other end is positioned in the vibration pallet of vortex agitator.Open vacuum pump and vortex agitator, vacuum pressure-0.09MPa, vibration 45min removes negative pressure of vacuum, and the failure of oscillations is taken out polylactic acid bracket, dry 24h.
Porous hydroxyapatite (size in hole is 350-550 μ m) and paclitaxel aqueous solution are placed the pyriform reaction vessel, and the reaction vessel opening links to each other with vacuum pump through the vacuum adapter of band piston.The other end is positioned in the vibration pallet of vortex agitator.Open vacuum pump and vortex agitator, vacuum pressure-0.09MPa, vibration 30min removes negative pressure of vacuum, and the failure of oscillations is taken out the hydroxyapatite support, dry 24h.
Porous hydroxyapatite (size in hole is 350-550 μ m) and amycin aqueous solution are placed the pyriform reaction vessel, and the reaction vessel opening links to each other with vacuum pump through the vacuum adapter of band piston.The other end is positioned in the vibration pallet of vortex agitator.Open vacuum pump and vortex agitator, vacuum pressure-0.08MPa, vibration 30min removes negative pressure of vacuum, and the failure of oscillations is taken out the hydroxyapatite support, dry 24h.
Porous hydroxyapatite (size in hole is 350-550 μ m) and amycin aqueous solution are placed the pyriform reaction vessel, and the reaction vessel opening links to each other with vacuum pump through the vacuum adapter of band piston.The other end is positioned in the vibration pallet of vortex agitator.Open vacuum pump and vortex agitator, vacuum pressure-0.09MPa, vibration 5min removes negative pressure of vacuum, and negative pressure is added in the failure of oscillations, the 5min that then vibrates, negative pressure is removed in the failure of oscillations, takes out the hydroxyapatite support, dry 48h.
Embodiment 7
Porous polylactic-co-glycolic acid copolymer support (size in hole is 150-300 μ m) and hydroxyapatite aqueous solution are placed the pyriform reaction vessel, and the reaction vessel opening links to each other with vacuum pump through the vacuum adapter of band piston.The other end is positioned in the vibration pallet of vortex agitator.Open vacuum pump and vortex agitator, vacuum pressure-0.09MPa, vibration 10min removes negative pressure of vacuum; The failure of oscillations connects negative pressure more again, and the 15min that then vibrates removes negative pressure then; The failure of oscillations is taken out polylactic-co-glycolic acid copolymer support, dry 24h.
Claims (10)
1. the method for a porous support load material is characterized in that concrete steps are: with porous support with mix by the load material or by the solution of load material; Constantly placing under the negative pressure in the vibration then, making loaded article or loaded article solution be full of the hole of internal stent, making the load material be carried in the middle of the support through modes such as absorption; Dry support promptly obtains the porous support of load material.
2. the method for porous support load material according to claim 1; The host material that it is characterized in that said porous support is any in the following macromolecular material: poly DL-lactide, gather the L-lactide, gather Acetic acid, hydroxy-, bimol. cyclic ester, the copolymer of polylactic acid-glycolic guanidine-acetic acid, poly 3-hydroxy butyrate, polyhydroxyalkanoate, poly-epsilon-caprolactone, gather ε-alkyl and replace caprolactone, gather δ-Wu Neizhi, Merlon, poe, polymethyl methacrylate, gather dioxanone, gather dioxane, polyether ester; Polystyrene, polrvinyl chloride, polyacrylate, polymethacrylates, gathering carbonic acid vinegar, nylon, polyurethane, polyformaldehyde, polyvinyl alcohol, polyvinyl acetate, polysiloxanes, perhaps is any in any type of copolymer or the blend of above-mentioned each kind polyester;
Perhaps be: non-metal inorganic material pottery, glass, carbon element, or these mixtures of material, or the mixture of these materials and additive.
Perhaps be: the composite of above-mentioned macromolecular material and non-metal inorganic material.
3. the method for porous support load material according to claim 1 and 2, it is characterized in that said is medicine by the load material, perhaps is polypeptide and protein substance, perhaps is water-soluble chitosan, hydroxyapatite, tricalcium phosphate or derivatives thereof.
4. the method for porous support load material according to claim 3 is characterized in that said medicine comprises: plain type of cephalo, penicillin medicine; Quinolones hydrochlorate or acylate, clindamycin hydrochloride or other salt, azithromycin; Roxithromycin; Paclitaxel, famotidine, oleanolic acid;
Said polypeptide and protein substance comprise: casein, gelatin, electrophoresis gamma Globulin, human albumin, Sanguis Bovis seu Bubali albumin.
5. the method for porous support load material according to claim 1; It is characterized in that said negative pressure adopts vacuum oil pump or vacuum pump to produce, the vacuum pressure scope is 0～-0.1MPa, pressure 1min-24h action time; Be pressurised into uninterrupted pressurization, or for being interrupted pressurization.
6. the method for porous support load material according to claim 1 is characterized in that used vibration is produced by vortex vibrating machine, cyclotron oscillation machine or disk vibration machine, vibrates for continuous oscillation perhaps is intermittent oscillation, and duration of oscillation is 1S-24h.
7. the method for porous support load material according to claim 1 is characterized in that said dry support uses lyophilization or vacuum drying mode, drying time 1-96h.
8. one kind is used for the device of porous support load substance method according to claim 1; It is characterized in that by be loaded with porous support and by the load material or by the load substance solution container, vibrating device, vacuum equipment form; Said container one end sealing; One end opening, blind end places vibrating device, and opening links to each other with the vacuum equipment sealing.
9. device according to claim 8 is characterized in that bottle or the pipe of said container for being processed by one or more materials of glass, pottery, macromolecular material, metal material.
10. device according to claim 8 is characterized in that the opening of said container links to each other through joint sealing with vacuum equipment, and joint is for the vacuum adapter of band piston or for not with the joint of piston.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201110196769A CN102327185A (en) | 2011-07-14 | 2011-07-14 | Method and device for loading substance with porous bracket |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110196769A CN102327185A (en) | 2011-07-14 | 2011-07-14 | Method and device for loading substance with porous bracket |
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| CN102327185A true CN102327185A (en) | 2012-01-25 |
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| CN201110196769A Pending CN102327185A (en) | 2011-07-14 | 2011-07-14 | Method and device for loading substance with porous bracket |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103110979A (en) * | 2013-02-09 | 2013-05-22 | 复旦大学 | High molecular porous material with surface deposited bone-like hydroxyapatite as well as preparation method and application thereof |
| CN114602723A (en) * | 2022-05-10 | 2022-06-10 | 北京理贝尔生物工程研究所有限公司 | Negative pressure medicine carrying device |
Citations (4)
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|---|---|---|---|---|
| CN2274983Y (en) * | 1996-03-07 | 1998-02-25 | 侯文富 | Timing vortex mixer |
| CN101234201A (en) * | 2008-01-24 | 2008-08-06 | 上海交通大学 | High molecule impregnation implantation sustained-release drug delivering system and preparation thereof |
| CN101406713A (en) * | 2007-10-12 | 2009-04-15 | 微创医疗器械(上海)有限公司 | Artificial blood vessel bracket and preparation method thereof |
| CN101920043A (en) * | 2010-08-17 | 2010-12-22 | 复旦大学 | Porous bracket with micro grooves on pore walls and preparation method thereof |
-
2011
- 2011-07-14 CN CN201110196769A patent/CN102327185A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2274983Y (en) * | 1996-03-07 | 1998-02-25 | 侯文富 | Timing vortex mixer |
| CN101406713A (en) * | 2007-10-12 | 2009-04-15 | 微创医疗器械(上海)有限公司 | Artificial blood vessel bracket and preparation method thereof |
| CN101234201A (en) * | 2008-01-24 | 2008-08-06 | 上海交通大学 | High molecule impregnation implantation sustained-release drug delivering system and preparation thereof |
| CN101920043A (en) * | 2010-08-17 | 2010-12-22 | 复旦大学 | Porous bracket with micro grooves on pore walls and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| LORENZO SOLETTI 等: "A seeding device for tissue engineered tubular structures", 《BIOMATERIALS》, vol. 27, no. 28, 31 October 2006 (2006-10-31) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103110979A (en) * | 2013-02-09 | 2013-05-22 | 复旦大学 | High molecular porous material with surface deposited bone-like hydroxyapatite as well as preparation method and application thereof |
| CN103110979B (en) * | 2013-02-09 | 2015-06-17 | 复旦大学 | High molecular porous material with surface deposited bone-like hydroxyapatite as well as preparation method and application thereof |
| CN114602723A (en) * | 2022-05-10 | 2022-06-10 | 北京理贝尔生物工程研究所有限公司 | Negative pressure medicine carrying device |
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Application publication date: 20120125 |