CN102323312A - Detection method and device for portable rapid visual detection of copper ion content - Google Patents
Detection method and device for portable rapid visual detection of copper ion content Download PDFInfo
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- CN102323312A CN102323312A CN201110281787A CN201110281787A CN102323312A CN 102323312 A CN102323312 A CN 102323312A CN 201110281787 A CN201110281787 A CN 201110281787A CN 201110281787 A CN201110281787 A CN 201110281787A CN 102323312 A CN102323312 A CN 102323312A
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Abstract
The invention relates to a detection method for portable rapid visual detection of a copper ion content, and the method comprises the following steps: a. preparing a simple circuit, generating a platinum electrode of -0.65 V; b. preparing 13-nm gold nanoparticles; c. centrifuging the gold nanoparticles obtained in step b, dispersing in secondary water; d. obtaining azide-functionalized gold nanoparticles; e. obtaining alkynyl-functionalized gold nanoparticles; f. mixing the functionalized gold nanoparticles prepared in step d and step e, adding a sample solution to be detected; g. putting the potentiostatic platinum electrode obtained in step a into the solution of step f, performing electrolysis within 10 min, observing the color change of the gold nanoparticles. The device of the invention is protable, can realize the rapid and visual detection of the copper ion content in the sample, has strong maneuverability, and has the advantage of simple apparatus.
Description
Technical field
The present invention relates to a kind of portable fast, the detection method and the device thereof of visual detection content of copper ion, belong to analytical chemistry, environmental monitoring field.
Background technology
Cu is an indispensable the third-largest trace element in the human body.The general chemical index of bivalent cupric ion is 1.0 mg/L in the potable water.Copper ion can through drink, bioconcentration gets in the human body body, causes cough, feels sick diarrhoea, even cause spinoffs such as liver function and kidney disorder.Therefore the detection of Cu is vital.Usually the method that detects Cu has atomic absorption spectrography (AAS), x-ray fluorescence spectrometry method and Electrochemical Detection or the like, but in these methods; The method cost that has is high; Instrument is expensive, complicated operation, and the method selectivity that has is bad; Need complicated actual sample preprocessing process, this just is necessary that seeking method simple, high selectivity realizes the detection to Cu.
Click chemistry is the new method of a kind of carbon heteroatoms link of calendar year 2001 Nobel laureate Sharpless proposition; Wherein general one type of reaction is the triazo-compound of Cu (I) catalysis and the addition reaction of alkynyl compounds; This reaction conditions is gentle, productive rate is high, accessory substance is few, separate and purify simply, thereby is widely applied to the every field such as synthetic, functional polymer, biomacromolecule, finishing, cell, virus of biological medicine.
Traditional method is to obtain Catalysts Cu (I) through the electronation of Cu (II), and then catalysis click chemical reaction, but this method needs long time just can detect the variation of signal usually, is not suitable for the original position fast detecting.This patent then is under certain voltage, copper ion to be reduced to monovalence copper to come catalysis to click chemical reaction, has accelerated reaction velocity greatly, has shortened the reaction time.The monovalence copper that produces impels the gold nano grain of azide functionalization and the gold nano grain of alkynyl functionalization to take place to click chemical reaction, is linked at together, thus the reunion of initiation gold nano grain.In the regular hour, the coagulation degree of the gold nano grain that the copper of variable concentrations causes is different, and change color is also different, can come the content of the copper ion in the observation sample through change in color.
Summary of the invention
The object of the present invention is to provide a kind of portable fast, the detection method and the device thereof of visual detection content of copper ion, help convenient, quick, the visual and simple structure of realizing that copper ion detects in the water.
One of characteristic of the present invention is: a kind of portable fast, the detection method of visual detection content of copper ion, it is characterized in that, carry out as follows:
A. make a ball bearing made using through battery and resistance, make it can produce the platinum electrode of the constant potential of-0.65 V;
B. the gold nano grain for preparing 13 nm;
C. centrifugal being distributed to again in the secondary water of gold nano grain that step b is obtained, and to regulate its pH be 7.0-8.0;
D. the gold nano grain that step c is obtained adds the sulfydryl chain CH that is modified with azido group
3(CH
2)
7SH, mixing carries out eccentric cleaning after the cultivation, obtain the gold nano grain of azide functionalization;
E. the gold nano grain that step c is obtained adds the sulfydryl chain HS (CH that is modified with alkynyl group
2)
15COOH, mixing adds HC ≡ CCH again after the cultivation
2NH
2,Carry out eccentric cleaning after the reaction, obtain the gold nano grain of alkynyl functionalization;
F. the gold nano grain with the functionalization of steps d and step e preparation mixes, and adds in the solution of sample of required detection;
G. the platinum electrode of the constant potential that forms among the step a is put into the solution of step f, in 10min, carried out electrolysis, observe the change color of gold nano grain, whether exceed standard with this content of judging copper ion in the sample.
Another feature of the present invention is: a kind of portable fast, the pick-up unit of visual detection content of copper ion, it is characterized in that: comprise putting into the test solution groove that detects solution is housed in order to the circuit of the constant potential platinum electrode that produces-0.65 V with platinum electrode.
Advantage of the present invention: the present invention is easy to carry about with one, can the time fast, the content of copper ion in the visual detection sample, and workable, have the simple advantage of instrument.
Description of drawings
Fig. 1 is the structural representation of pick-up unit of the present invention.
Fig. 2 is the circuit diagram of pick-up unit of the present invention.
Embodiment
A kind of portable fast, the detection method of visual detection content of copper ion, carry out as follows:
A. make a ball bearing made using through battery and resistance, make it can produce the platinum electrode of the constant potential of-0.65 V;
B. the gold nano grain for preparing 13 nm;
C. centrifugal being distributed to again in the secondary water of gold nano grain that step b is obtained, and to regulate its pH be 7.0-8.0;
D. the gold nano grain that step c is obtained adds the sulfydryl chain CH that is modified with azido group
3(CH
2)
7SH, mixing carries out eccentric cleaning after the cultivation, obtain the gold nano grain of azide functionalization;
E. the gold nano grain that step c is obtained adds the sulfydryl chain HS (CH that is modified with alkynyl group
2)
15COOH, mixing adds HC ≡ CCH again after the cultivation
2NH
2,Carry out eccentric cleaning after the reaction, obtain the gold nano grain of alkynyl functionalization;
F. the gold nano grain with the functionalization of steps d and step e preparation mixes, and adds in the solution of sample of required detection;
G. the platinum electrode of the constant potential that forms among the step a is put into the solution of step f, in 10min, carried out electrolysis, observe the change color of gold nano grain, whether exceed standard with this content of judging copper ion in the sample.
With reference to figure 1 and Fig. 2, a kind of portable fast, the pick-up unit of visual detection content of copper ion, comprise putting into the test solution groove 2 that detects solution be housed in order to the circuit 1 of the constant potential platinum electrode 3 that produces-0.65 V with platinum electrode 3.
Foregoing circuit 1 comprises direct supply, adjustable resistance, switch and platinum electrode 3, the positive electricity end ground connection of said direct supply.
The above person is merely preferred embodiment of the present invention, and all changes of doing according to technical scheme of the present invention when the function that is produced does not exceed the scope of technical scheme of the present invention, all belong to protection scope of the present invention.
Claims (3)
- One kind portable fast, the detection method of visual detection content of copper ion, it is characterized in that, carry out as follows:A. through ball bearing made using of battery and resistance making, make it can produce-platinum electrode of the constant potential of 0.65V;B. the gold nano grain for preparing 13nm;C. centrifugal being distributed to again in the secondary water of gold nano grain that step b is obtained, and to regulate its pH be 7.0-8.0;D. the gold nano grain that step c is obtained adds the sulfydryl chain CH that is modified with azido group 3(CH 2) 7SH, mixing carries out eccentric cleaning after the cultivation, obtain the gold nano grain of azide functionalization;E. the gold nano grain that step c is obtained adds the sulfydryl chain HS (CH that is modified with alkynyl group 2) 15COOH, mixing adds HC ≡ CCH again after the cultivation 2NH 2,Carry out eccentric cleaning after the reaction, obtain the gold nano grain of alkynyl functionalization;F. the gold nano grain with the functionalization of steps d and step e preparation mixes, and adds the sample solution of required detection;G. the platinum electrode of the constant potential that forms among the step a is put into the solution of step f, in 10min, carried out electrolysis, observe the change color of gold nano grain, whether exceed standard with this content of judging copper ion in the sample.
- One kind portable fast, the pick-up unit of visual detection content of copper ion, it is characterized in that: comprise putting into the test solution groove that detects solution is housed in order to the circuit of the constant potential platinum electrode that produces-0.65 V with platinum electrode.
- 3. according to claim 2 portable fast, the pick-up unit of visual detection content of copper ion, it is characterized in that: said circuit comprises direct supply, adjustable resistance, switch and platinum electrode, the positive electricity end ground connection of said direct supply.
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| CN201110281787A CN102323312A (en) | 2011-09-21 | 2011-09-21 | Detection method and device for portable rapid visual detection of copper ion content |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102944590A (en) * | 2012-11-21 | 2013-02-27 | 济南大学 | Preparation of bionic molecular imprinting electrochemical sensor and based on click chemistry and detection of food allergen |
| CN103278497A (en) * | 2013-05-15 | 2013-09-04 | 中国科学院烟台海岸带研究所 | Copper ion colorimetry detecting kit and detecting method thereof |
| CN103529022A (en) * | 2012-07-06 | 2014-01-22 | 南京大学 | Method for quick visual detection of copper ion content based on quantum photoelectric effect |
| CN104089950A (en) * | 2014-07-16 | 2014-10-08 | 国家纳米科学中心 | Method for visually detecting antigen-antibody reaction and application of method |
| CN104914100A (en) * | 2015-06-04 | 2015-09-16 | 苏州大学张家港工业技术研究院 | Site rapid bacterium visual detection method and site rapid bacterium visual detection kit |
| CN105823770A (en) * | 2016-05-25 | 2016-08-03 | 武汉大学 | Optical-interference-free Raman labeling probe and preparation method and application thereof |
| CN109622944A (en) * | 2019-01-11 | 2019-04-16 | 临沂大学 | A kind of preparation method and applications of functionalization gold nanoparticle and preparation method thereof, gold nanoparticle dimer |
| CN111721886A (en) * | 2020-07-09 | 2020-09-29 | 福州大学 | Stimulation-wetting-responsive copper ion detection capillary tube and application thereof |
| CN113092309A (en) * | 2021-04-13 | 2021-07-09 | 福州大学 | Capillary height indicator device and application thereof in detecting hydrogen sulfide |
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| US6372509B1 (en) * | 1998-03-18 | 2002-04-16 | The United States Of America As Represented By The Secretary Of The Navy | Situ copper (I) |
| CN101435778A (en) * | 2007-11-12 | 2009-05-20 | 国家纳米科学中心 | Method for qualitatively detecting CU<2+> in solution directly by eye |
| CN102175675A (en) * | 2011-01-20 | 2011-09-07 | 福州大学 | Method for detecting copper ions |
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2011
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| US6372509B1 (en) * | 1998-03-18 | 2002-04-16 | The United States Of America As Represented By The Secretary Of The Navy | Situ copper (I) |
| CN101435778A (en) * | 2007-11-12 | 2009-05-20 | 国家纳米科学中心 | Method for qualitatively detecting CU<2+> in solution directly by eye |
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|---|
| YANG ZHOU等: "Visual Detection of Copper(II)by Azide-and Alkyne-Functionalized", 《ANGEW.CHEM.INT.ED》, vol. 47, no. 39, 15 September 2008 (2008-09-15), pages 7454 - 7455 * |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103529022A (en) * | 2012-07-06 | 2014-01-22 | 南京大学 | Method for quick visual detection of copper ion content based on quantum photoelectric effect |
| CN102944590A (en) * | 2012-11-21 | 2013-02-27 | 济南大学 | Preparation of bionic molecular imprinting electrochemical sensor and based on click chemistry and detection of food allergen |
| CN103278497A (en) * | 2013-05-15 | 2013-09-04 | 中国科学院烟台海岸带研究所 | Copper ion colorimetry detecting kit and detecting method thereof |
| CN103278497B (en) * | 2013-05-15 | 2015-07-01 | 中国科学院烟台海岸带研究所 | Copper ion colorimetry detecting kit and detecting method thereof |
| CN104089950B (en) * | 2014-07-16 | 2017-12-26 | 国家纳米科学中心 | A kind of method and its application of Visual retrieval antigen-antibody reaction |
| CN104089950A (en) * | 2014-07-16 | 2014-10-08 | 国家纳米科学中心 | Method for visually detecting antigen-antibody reaction and application of method |
| CN104914100A (en) * | 2015-06-04 | 2015-09-16 | 苏州大学张家港工业技术研究院 | Site rapid bacterium visual detection method and site rapid bacterium visual detection kit |
| CN105823770A (en) * | 2016-05-25 | 2016-08-03 | 武汉大学 | Optical-interference-free Raman labeling probe and preparation method and application thereof |
| CN109622944A (en) * | 2019-01-11 | 2019-04-16 | 临沂大学 | A kind of preparation method and applications of functionalization gold nanoparticle and preparation method thereof, gold nanoparticle dimer |
| CN109622944B (en) * | 2019-01-11 | 2020-11-06 | 临沂大学 | Functionalized gold nanoparticles and preparation method thereof, and preparation method and application of gold nanoparticle dimer |
| CN111721886A (en) * | 2020-07-09 | 2020-09-29 | 福州大学 | Stimulation-wetting-responsive copper ion detection capillary tube and application thereof |
| CN111721886B (en) * | 2020-07-09 | 2021-07-13 | 福州大学 | Stimulation-wetting-responsive copper ion detection capillary tube and application thereof |
| CN113092309A (en) * | 2021-04-13 | 2021-07-09 | 福州大学 | Capillary height indicator device and application thereof in detecting hydrogen sulfide |
| CN113092309B (en) * | 2021-04-13 | 2022-01-28 | 福州大学 | Capillary height indicator device and application thereof in detecting hydrogen sulfide |
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Application publication date: 20120118 |