CN102309001B - Food composition and medicinal composition of lactobacillus strain for treating allergy - Google Patents
Food composition and medicinal composition of lactobacillus strain for treating allergy Download PDFInfo
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Abstract
The invention relates to a novel lactobacillus strain and a novel composition which contains an unknown strain which can be used for enhancing the anti-allergic capability. The composition can exist in the form of food or medicinal composition. A method for screening and testing the lactobacillus strain composition for enhancing the Th1 immunological competence and regulate overreacting Th2 immunologic reaction caused by allergy is also disclosed.
Description
Technical field
The present invention is relevant a kind of food compositions and medical composition, particularly a kind of food compositions and medical composition that comprises antianaphylactic lactic bacterium strains.
Background technology
The general edible product that contains milk-acid bacteria (LAB) only has the health effect of adjusting enteron aisle, although there is ten hundreds of lactic bacterium strains to be present in nature, only has the minority lactobacillus strain to have antianaphylactic speciality.The ability of the acidproof and bile tolerance ability that these bacterial strains of minority have, absorption mucous membrane epidermic cell and the characteristics such as ability that still can survive after by intestines and stomach are the important evidence of screening when the bacterial strain of promotion health effect is arranged.Even to this day, only there is the verified lactic bacterium strains that it has the antianaphylaxis health effect of several strains to be identified out, and milk-acid bacteria is the specificity of bacterial strain (strain) but not bacterial classification (species) to healthy function, and this kind has the bacterial strain of special efficacy to be called functional probiotic bacterium (Guidelines for the evaluation of probiotics in food for the healthy of people; Report of j oint FAO/WHOworking group on drafting guidelines for the evaluation of probiotics in food; London Ontario, Canada April 30and May 1,2002:1-7).
Many milk-acid bacterias reported in literature relevant with anaphylaxis arranged both at home and abroad.Carry out aspect the zooperal research lowering irritated ability for milk-acid bacteria, the animal experiment that the dead milk-acid bacteria L.plantarum L-137 of thermic does can induce the scavenger cell of DBA/2 mouse and spleen cell to produce IL-12 and IFN-γ, and can suppress to have had the narrow spectrum IgE of casein (casein), increase is to the narrow spectrum IgG1 antibody of casein (Murosaki, 1998).Around OVA-TCR-Tg mouse feeding L.casei Shirota, increase spleen cell secretion of gamma-IFN and IL-12, reduce secretion IL-4 and IL-5 (Shida et al., 2002).OVA sensitization BALB/c mouse feeding milk-acid bacteria Lactobacillus fermented milk 27 days can reduce and has the OVA specific IgE in the serum (Ishida et al., 2003).The C3H/Hej mouse is through OVA and Toxins,exo-, cholera (cholera toxin) sensitization, feeding B.bifidum BGN4, L.casei 911, can make tool OVA specific IgE in the serum, IgA and total IgE, IgG1 reduce (Kim et al., 2005), these documents confirm on the animal experiment of irritated pattern, the feeding milk-acid bacteria can reduce the content of IgE in the serum, improves by this irritated situation.
Milk-acid bacteria is in clinical experiment, patient with anaphylactic disease can improve anaphylaxis really, merge and take dry milk-acid bacteria L.rhamnosus and six weeks of L.reuteri, helpful to anaphylactic disease, assess probiotic bacterium to one to 13 years old children's atopic dermatitis with the survey head of a family, find that children's atopic dermatitis of 56% is improved (Rosenfeldt et al., 2003).Ishida (2005) scholar drinks L.acidophilus strain L-92 for 49 Allergic Rhinitis, finds that also allergic symptom is improved.Take L.fermentum VRI eight weeks of 003 PCCTM, 53 children with atopic dermatitis symptom can increase PBMC cell generation IFN-γ, and improve irritated situation (Prescott et al., 2005).
(2006) etc. scholar's research points out to have the test that the atopic dermatitis patient does for 54, take L.rhamnosus strain GG and placebo after, can effectively improve allergic symptom.
Research points out that lactic-acid bacteria cells wall composition has immunoregulation capability, comprises Peptidoglycans (PG), Lipoteichoic acids (LTA), Phosphopolysaccharide.For example the Phosphopolysaccharide in the L.lactis cell walls can stimulate the mouse white cell to produce IFN-γ, and Teichoic acid and Muramyl dipeptide increase stimulates PBMC to produce IFN-γ (Cross et al., 2001); L.paracasei KW3110 cell wall constituent LTA can affect NF-κ B signal pipeline, has very strong immunoregulation ability (Fujiwara et al., 2004).
Probiotic strain has good efficacy for human body, and has many possible mechanisms to be suggested to illustrate why probiotic strain has desirable influence to human body.These mechanism mostly concentrate on improves the immunity system that intestinal mucosa cell function of shielding directly affects human body, the balance between the secretion of inflammatory cells hormone before for example suppressing, the activity that affects adjustment type T cell and the adjustment Th1/Th2.Improving the bacterium of adjusting the intestines and stomach fungal component period the infant is very important mutually, and infant's enteron aisle of many allergy lacks the probiotic bacterium flora, and Intestinal flora all has more aerogenesis folder film clostridium and less bifidus flora.Therefore there is enteron aisle in the probiotic bacterium clump, for the immunological competence construction of human body and to possess sound immunoregulation ability be very important key.Yet, the mechanism that affects human body for probiotic bacterium still not yet is finalized, for this point, in the development in future, still need more external and in vivo test to go explanation, the mechanism that human body is had a so good impact why and research be applied to optimal conditions on the human body (Michael et al., 2008).
Summary of the invention
Comprising by following any biological culture of one aspect of the present invention broad sense: lactobacillus reuteri (Lactobacillus reuteri) GL-104 bacterial strain, Chinese Typical Representative culture collection center preserving number M209138 bacterial strain, and physiologically acceptable the vehicle food compositions or the medical composition that form.
In another specific embodiment, the biological culture that comprises the of the same race or mutant of physiologically acceptable following any bacterial strain that strengthens the antianaphylaxis ability: lactobacillus reuteri (Lactobacillus reuteri) GL-104 bacterial strain, Chinese Typical Representative culture collection center preserving number M 209138.
Again, in another specific embodiment, but comprising via promoting cell IL-12 (IL-12) or interferon-gamma (IFN-gamma) to regulate and control Th1 type immune response and Immunosuppression sphaeroprotein IgE of the present invention's broad sense, improve the excessive immunoreactive allergic phenomena of Th2 type, it is that Mammals is given to reach any the aforementioned biological culture that stimulates Th1 type immune effect dosage.
But the present invention is comprising in the application's case specification sheets separately or part, constitutive requirements and the feature of sum total explanation of broad sense also, reach any any or all of combination any or multiple in these parts, constitutive requirements and the feature that comprises, if and described herein and clear and definite complete things when known coordinator in the related art techniques relevant with the present invention, having occurred, these known coordinators will be incorporated herein as independent item.
Description of drawings
Fig. 1 shows, when respectively with 10
6To 10
8The lactobacillus reuteri GL-104 bacterial strain M 209138 and 10 of cfu
5To 10
7Individual mouse spleen cell (Splenocyte) co-cultivation 48 hours is collected cell conditioned medium liquid, with the content of enzyme-linked immunosorbent assay detecting interferon-gamma (IFN-gamma) in supernatant liquor.Wherein take without antianaphylaxis function milk-acid bacteria (Lactobacillus casei BCRC17001) and Experimental Background value (Cell only) as negative control group, prove its milk-acid bacteria that antianaphylaxis effect is arranged (Lactobacillusrhamnosus GG BCRC 16000) and PHA as the positive control group to deliver most literature, detecting interferon-gamma irriate concentration.The result shows that test group can stimulate significantly mouse spleen cell (Splenocyte) secretion interferon-gamma and negative control group (Lactobacillus casei BCRC17001) that significant difference is arranged and exceeds nearly 4 times than the quantity of stimulus of positive control group (Lactobacillusrhamnosus GG BCRC 16000).
Fig. 2 shows, when respectively with 10
6To 10
8The lactobacillus reuteri GL-104 bacterial strain M 209138 and 10 of cfu
5To 10
7Individual human peripheral blood mononuclear cell (PBMC) co-cultivation 48 hours is collected cell conditioned medium liquid, with the content of enzyme-linked immunosorbent assay detecting interferon-gamma (IFN-gamma) in supernatant liquor.Wherein take without antianaphylaxis function milk-acid bacteria (Lactobacillus casei BCRC17001) and Experimental Background value (Cell only) as negative control group, prove its milk-acid bacteria that antianaphylaxis effect is arranged (Lactobacillusrhamnosus GG BCRC 16000) and PHA as the positive control group to deliver most literature, detecting interferon-gamma irriate concentration.The result shows that stimulating human peripheral blood mononuclear cell (PBMC) secretion interferon-gamma and negative control group (Lactobacillus casei BCRC17001) have significant difference and exceed 2.2 times than the quantity of stimulus of positive control group (Lactobacillus rhamnosus GG BCRC 16000) test group significantly.
Fig. 3 shows, when respectively with thermic dead 10
6To 10
8The lactobacillus reuteri GL-104 bacterial strain M 209138 and 10 of cfu
5To 10
7Individual human dcs (dendritic cell) co-cultivation 48 hours is collected cell conditioned medium liquid, with the content of enzyme-linked immunosorbent assay detecting cell interleukin 12 p40 in supernatant liquor.Wherein take without antianaphylaxis function milk-acid bacteria (Lactobacillus casei BCRC17001) and Experimental Background value (Cell only) as negative control group, prove its milk-acid bacteria that antianaphylaxis effect is arranged (Lactobacillus rhamnosus GG BCRC 16000) and LPS as the positive control group to deliver most literature, detecting cell interleukin 12 p40 (IL-12p40) irriate concentration.The result show test group significantly stimulating human dendritic cell secretory cell interleukin 12 p40 (IL-12p40) and negative control group (Lactobacillus casei BCRC17001) significant difference is arranged, and exceed 2 times than the quantity of stimulus of positive control group (Lactobacillus rhamnosus GG BCRC 16000).
Fig. 4 shows, lactobacillus reuteri GL-104 bacterial strain M 209138 is after the activation of substratum, total count is via simulation hydrochloric acid in gastric juice solution and cholate solution-treated different time, the bacterium number of lactobacillus reuteri GL-104 bacterial strain M 209138 is not affected by hydrochloric acid in gastric juice and cholate and sharply descends, and proves that antianaphylaxis lactic bacterium strains of the present invention can be by the test of the strict environment of digestion.
Fig. 5 shows, according to the analytical procedure of the scholars such as Jacobsen (1999), when the average bacterium number in each visual field is less than 40, is judged to be non-cohesive (nonadhesive); When the average bacterium number in each visual field between 41 to 100 the time, be judged to be and adhere to (adhesive); When the average bacterium number in each visual field greater than 100 the time, be judged to be strong adhesion (strongly adhesive).Show that lactobacillus reuteri GL-104 bacterial strain M 209138 is judged to be " strong adhesion ".
Embodiment
One aspect of the present invention broad sense comprise following biological culture: lactobacillus reuteri (Lactobacillus reuteri) GL-104 bacterial strain, Chinese Typical Representative culture collection center preserving number M 209138, and physiologically acceptable vehicle or the thinner food compositions or the medical composition that form.
Many documents are pointed out to increase the secretion increase that the first type T cell hormone comprises cell interleukin (IL-12), interferon-gamma (IFN-gamma), can effectively improve allergic symptom; Further class tongued bell acceptor (Toll-like receptor) combination on specific lactobacillus strain and the dendritic cell is also pointed out in research, the albumen of translating in the active cells moves in the nuclear and discharges a large amount of cytohormones, a ring that belongs to congenital immunity, therefore some specific lactic bacterium strains is by its cell wall polysaccharides class material such as peptidoglycan (peptidoglycan), saccharan (polysaccharide) etc., through innate immune system, really can activate the growth of T cell in the body.
The lyophilize culture of bacterial strain of the present invention has been deposited in Chinese Typical Representative culture collection center, and the address is China, Wuhan, Wuhan University 430072.The detail file of preservation are as shown in table 1:
Table 1
| The bacterial strain name | Numbering | Date |
| Lactobacillus reuteri GL-104 | M 209138 | On 08 14th, 2009 |
Found to have antianaphylactic ability such as this strains of lactic acid bacteria of the listed preservation of table 1, comprised that the symptom for atopic dermatitis, urticaria, allergic rhinitis, food anaphylaxis and asthma has the function of slowing down and treating.
Embodiment 1: the morphology of antianaphylaxis milk-acid bacteria and general aspects.
Confirm the feature of bacterial strain on taxonomy according to 16S rDNA sequential analysis and API Bacteria Identification systems analysis result.Find that Feng Hua strain number GL-104 is lactobacillus reuteri.The feature of this bacterial strain on morphology and general aspects listed in table 2 in detail:
Table 2
| The bacterial strain name | Morphological specificity |
| Lactobacillus reuteri GL-104 | 1. when the MRS nutrient solution was cultivated, thalline was rod-short, and two ends are rounded, usually separately appearance, paired or one-tenth short chain shape.2. the Gram-positive bacillus does not generate spore, does not have catalase, oxydase and a mobility, all can grow at aerobic and anaerobic environment, and optimum growth temperature is 37 ± 1 ℃, belongs to facultative heterogeneous fermentable bacterial strain, does not produce gas during glucose metabolism. |
Embodiment 2: the antianaphylaxis milk-acid bacteria is to the effect of the Secretion regulation Th1 type immunological competence of promotion interferon-gamma.
Inspect this strain antianaphylaxis lactobacillus strain, the enhancement effect of 209138 pairs of Th1 types of lactobacillus reuteri GL-104 bacterial strain M immunological competence, it is to measure mouse spleen cell (Splenocyte) and human peripheral blood mononuclearcell (Peripheral blood mononuclear cell, PBMC) measure respectively the secretory volume of interferon-gamma after co-cultivation with aforementioned this strain antianaphylaxis milk-acid bacteria, verify the bacterial strain with antianaphylaxis ability.Use following experimental procedure:
1. get the each about spleen of suitable mouse spleen and human blood amount and 200mL whole blood.
2. after spleen being put into buffered soln and smashing, centrifugal 5 minutes of 100g, it is for subsequent use to get supernatant liquor.
3. the blood cell parting liquid (Ficoll-paque) of getting equal proportion and spleen cell supernatant liquor and blood at 18-20 ℃ with the centrifugal 30-40 of 400g minute.
4. get spleen cell layer and human peripheral blood leukocyte cell layer, after buffered soln cleans cell 2-3 time, with suitable substratum (for example RPMI-1640) suspension cell.
With cell with activated 3 days lactobacillus strain with 1: 10 ratio co-cultivation 48 hours.
6. collect the supernatant liquor of cell cultures.Utilize the content of enzyme-linked immunosorbent assay (ELISA) detecting interferon-gamma (IFN-gamma) in supernatant liquor.
The statistical study of data such as table 3, table 4 and Fig. 1, shown in Figure 2 represent with Mean ± SD.Figure 1 and Figure 2 is ought be respectively with 10
6To 10
8Cfu lactobacillus reuteri GL-104 bacterial strain M 209138 and 10
5To 10
7Individual spleen cell and human peripheral blood mononuclearcell (PBMC) difference co-cultivation 48 hours are collected cell conditioned medium liquid, with the content of enzyme-linked immunosorbent assay detecting interferon-gamma (IFN-gamma) in supernatant liquor.Wherein take without antianaphylaxis function milk-acid bacteria (Lactobacillus casei BCRC 17001) and Experimental Background value (Cell only) as negative control group, prove its milk-acid bacteria that antianaphylaxis effect is arranged (Lactobacillus rhamnosus GG B CRC 16000) and PHA (Phytohemagglutinin) as the positive control group to deliver most literature, detecting interferon-gamma irriate concentration.The result shows that test group can stimulate significantly mouse spleen cell (Splenocyte) and human peripheral blood mononuclearcell (PBMC) secretion interferon-gamma and negative control group (Lactobacillus casei BCRC 17001) that significant difference is arranged and exceeds nearly 4 times (Splenocyte) and nearly 2.2 times (PBMC) than the quantity of stimulus of positive control group (Lactobacillusrhamnosus GG BCRC 16000).Table 3 and table 4 are that this strain antianaphylaxis bacterial strain and control group are cultivated with mouse spleen cell and human peripheral blood mononuclearcell (PBMC) respectively, stimulate the secretory volume (means+SD) of interferon-gamma:
Table 3Splenocyte
Table 4PBMC
| Strain name | The secretory volume of interferon-gamma (pg/mL) |
| Positive control group (L.rhamnosus GG BCRC 16000) | 8201±322 |
| Lactobacillus reuteri GL-104M 209138 | 15826±549 |
| Negative control group (L.casei BCRC17001) | 533±153 |
| Positive control group (PHA) | 14560±1084 |
| Negative control group (Cell only) | 46±30 |
Embodiment 3: the antianaphylaxis milk-acid bacteria is to the effect (take dendritic cell dendritic cell as external effect verification platform) of the Secretion regulation Th1 type immunological competence of promotion cell interleukin 12 p40 (IL-12p40).
Inspect antianaphylaxis lactic bacterium strains of the present invention-lactobacillus reuteri GL-104 bacterial strain M209138 to the enhancement effect of Th1 type immunological competence, it is the secretory volume of measuring cell interleukin 12 p40 (IL-12p40) after mensuration human dcs (dendritic cell) and the aforementioned antianaphylaxis milk-acid bacteria co-cultivation, verifies the bacterial strain with antianaphylaxis ability.Use following experimental procedure:
1. extract at every turn about 200mL of suitable human blood amount.
2. the blood cell parting liquid (Ficoll-paque) of getting equal proportion with blood at 18-20 ℃ with the centrifugal 30-40 of 400g minute.
3. get human peripheral blood mononuclearcell (PBMC) layer, after buffered soln cleans cell 2-3 time, with suitable substratum (for example RPMI-1640) suspension cell.
4. human peripheral blood mononuclearcell (PBMC) cell is with CD14
+Microbeads (MiniMACSsystem) is with the CD14 of cell
+The monocyte purifying.
5. be divided into dendritic cell with cytohormone (IL-4) and tethelin (GM-CSF) irritation cell, 6-7 days incubation time is collected the dendritic cell of having broken up.
6. with the activation in 3 days before co-cultivation of antianaphylaxis lactic bacterium strains, afterwards with 100 ℃ of dead lactic bacterium strains of thermic 30 minutes.
7. the lactobacillus strain that dendritic cell and thermic is dead was with 1: 10 ratio co-cultivation 48 hours.
8. collect the supernatant liquor of cell cultures.Utilize the content of enzyme-linked immunosorbent assay (ELISA) detecting cell interleukin 12 p40 (IL-12p40) in supernatant liquor.
The statistical study of data such as table 5 and shown in Figure 3 represent with Mean ± SD.Figure 3 shows that ought be respectively with thermic dead 10
6To 10
8Cfu lactobacillus reuteri GL-104 bacterial strain M 209138 and 10
5To 10
7Individual human dcs co-cultivation 48 hours is collected cell conditioned medium liquid, with the content of enzyme-linked immunosorbent assay detecting cell interleukin 12 p40 (IL-12p40) in supernatant liquor.Wherein take without antianaphylaxis function milk-acid bacteria (Lactobacillus casei BCRC 17001) and Experimental Background value (Cell only) as negative control group, prove its milk-acid bacteria that antianaphylaxis effect is arranged (Lactobacillus rhamnosus GG BCRC16000) and LPS (Lipopolysaccharide) as the positive control group to deliver most literature, detecting cell interleukin 12 p40 (IL-12p40) irriate concentration.The result show test group significantly stimulating human dendritic cell secretory cell interleukin 12 p40 (IL-12p40) and negative control group (Lactobacillus casei BCRC 17001) significant difference is arranged, and exceed 2 times than the quantity of stimulus of positive control group (Lactobacillus rhamnosus GG BCRC 16000).Table 5 is depicted as respectively with the dead antianaphylaxis lactic bacterium strains of thermic and control group and dendritic cell and cultivates, the secretory volume of irritation cell interleukin 12 p40 (IL-12p40) (means ± SD):
Table 5
| Strain name | The secretory volume (pg/mL) of cell interleukin 12 p40 |
| Positive control group (L.rhamnosus GG BCRC 16000) | 6905±611 |
| Lactobacillus reuteri GL-104M 209138 | 14425±1741 |
| Negative control group (L.casei BCRC17001) | 51±2 |
| Positive control group (LPS) | 22103±780 |
| Negative control group (Cell only) | 20±147 |
Embodiment 4: the stomach juice-resistant cholate test of antianaphylaxis milk-acid bacteria.
Whether inspect antianaphylaxis lactobacillus strain of the present invention-lactobacillus reuteri GL-104 bacterial strain M 209138 and have by the ability of hydrochloric acid in gastric juice cholate test and bring into play its antianaphylactic function at enteron aisle smoothly, experiment process is as follows:
1. with antianaphylaxis milk-acid bacteria activation of the present invention 3 days.
2. get 1mL bacterium liquid and calculate original bacterium number, remaining milk-acid bacteria centrifugal 10 minutes with 500g adds the washed with de-ionized water milk-acid bacteria 2-3 time.
3. add and be deployed in the substratum of pH 2.5 with hydrochloric acid, antianaphylaxis milk-acid bacteria of the present invention places 37 ℃ of incubators with after the substratum of pH 2.5 fully mixes.
4. the bacterium liquid that per hour takes out 1mL with washed with de-ionized water 2-3 time after, the calculating survivaling cell number is till 3 hours incubation times.
5. residue bacterium liquid centrifuged deposit thing is again with Hui Rong in the substratum that contains 1.5% (w/V) oxgall (ox gall, Sigma), after fully mixing, in 37 ℃ of cultivations.
6. the bacterium liquid that per hour takes out 1mL with washed with de-ionized water 2-3 time after, the calculating survivaling cell number is till 4 hours incubation times.
7. record lactobacter growth speed is calculated milk-acid bacteria to the tolerance of hydrochloric acid in gastric juice and cholate, and so that antianaphylaxis milk-acid bacteria of the present invention is in the presence of hydrochloric acid in gastric juice and cholate in the comparative sample, whether strain growth is suppressed.
The capability result of stomach juice-resistant and analysis and arrangement are in table 6 and shown in Figure 4, result shown in Figure 4 shows, antianaphylaxis milk-acid bacteria of the present invention is after the substratum activation, bacterial strain is processed acidic buffer solution and cholate, lactobacillus reuteri GL-104 bacterial strain M 209138 bacterium numbers are not subjected to the impact of hydrochloric acid in gastric juice and cholate and sharply descend, and prove that antianaphylaxis milk-acid bacteria of the present invention can be by the test of the strict environment of digestion.Table 6 is that the hydrochloric acid with pH value 2.5 mixes test its stomach juice-resistant ability (Logmeans ± Log SD) with antianaphylaxis milk-acid bacteria of the present invention:
Table 6
| Strain name | Original bacterium number (Log cfu/mL) | pH2.5-1hr (Log cfu/mL) | pH2.5-2hr (Log cfu/mL) | pH2.5-3hr (Log cfu/mL) |
| Lactobacillus reuteri M 209138 | 9.33±0.012 | 9.15±0.15 | 8.92±0.28 | 8.49±0.14 |
The capability result of bile tolerance and analysis and arrangement are in table 7 and shown in Figure 4, and table 7 is that the bile (bile) with 1.5% mixes test its bile tolerance ability (Log means ± Log SD) with antianaphylaxis milk-acid bacteria of the present invention:
Table 7
| Strain name | Original bacterium number (Log cfu/mL) | 1.5％bile -1hr (Log | 1.5％bile -2hr (Log | 1.5％bile -3hr (Log | 1.5％bile -4hr (Log |
[0068]
| cfu/mL) | cfu/mL) | cfu/mL) | cfu/mL) | ||
| Lactobacillus reuteri M 209138 | 9.33±0.012 | 8.22±0.39 | 8.03±0.026 | 8.11±0.091 | 7.96±0.15 |
Embodiment 5: the antianaphylaxis milk-acid bacteria is to the adsorptive power test of human intestine's epidermic cell (Caco-2).
Adopt the cell strain that has broken up to analyze the ability of antianaphylaxis lactic bacterium strains absorption human intestine's epidermic cell of the present invention (Caco-2) in vitro.Elder generation, is inserted in the porous cell culture plate after growing to monolayer cell on the cover glass by the strain of human intestine's epidermic cell (Caco-2) monolayer cell.Then with cell: the ratio of milk-acid bacteria is 1: 200, co-cultivation 1-3 hour, with buffered soln clean and utilize the methyl alcohol fixed cell and remaining adhere to the bacterium number after, carry out gramstaining and determine accompanying bacterium number.With reference to the analytical procedure of the scholars such as Jacobsen (1999), 20 visuals field of counting when the average bacterium number in each visual field is less than 40, are judged to be non-cohesive (nonadhesive) under 1000 power microscopes; When the average bacterium number in each visual field between 41 to 100 the time, be judged to be and adhere to (adhesive); When the average bacterium number in each visual field greater than 100 the time, be judged to be strong adhesion (strongly adhesive).
The statistical study of data represents with Mean ± SD.On average, get the calculated value in 45 visuals field, its result puts in order in table 8 and Fig. 5.Fig. 5 shows that lactobacillus reuteri GL-104 bacterial strain M 209138 is judged to be " strong adhesion ".Table 8 is that antianaphylaxis milk-acid bacteria of the present invention is to the adsorptive power test of human intestine's epidermic cell (Caco-2) cell strain.
Table 8
| Strain name | Means±SD |
| Lactobacillus reuteri M 209138 | 204±58 |
Because milk-acid bacteria will be brought into play antianaphylactic effect except will finding out the bacterial strain with specific function, to confirm that more this bacterial strain can be by outside the environment of human body hydrochloric acid in gastric juice cholate, the lactic bacterium strains that also will can have to the small intestinal mucosa epidermic cell favourable absorption ability, also because of this specific character, antianaphylaxis milk-acid bacteria of the present invention just can provide the medical use for the treatment of or the allergic symptom of releiving.Antianaphylaxis milk-acid bacteria described in the invention can strengthen the anaphylaxis that Th1 approach regulation and control Th2 crosses Sheng simultaneously.A target of the present invention be exactly continue towards reach these demands or provide at least popular in the allergy treatment except steroid or antihistaminic new selection, the present invention finds out human body is had no side effect and wholesome antianaphylaxis milk-acid bacteria is used as the new selection of irritated treatment.
Above-described embodiment only is for technological thought of the present invention and characteristics are described, its purpose is to make those skilled in the art can understand content of the present invention and implements according to this, when can not with restriction claim of the present invention, the equalization of namely generally doing according to disclosed spirit changes or modifies, and must be encompassed in the claim of the present invention.
Claims (6)
1. food compositions comprises:
The lactic bacterium strains of immune stimulatory emiocytosis antianaphylaxis relevant cell hormone: lactobacillus reuteri (Lactobacillus reuteri) GL-104 bacterial strain, Chinese Typical Representative culture collection center preserving number M 209138; And
Physiologically acceptable vehicle.
2. food compositions as claimed in claim 1, wherein this vehicle is a kind of food.
3. food compositions as claimed in claim 2, wherein this food comprises fermented-milk, cheese, milk powder, tea, coffee or above combination.
4. food compositions as claimed in claim 1, wherein this lactic bacterium strains is for having the bacterial strain of activity or deactivation (inactivated).
5. one kind is used for the treatment of irritated medical composition, comprises:
The lactic bacterium strains of immune stimulatory emiocytosis antianaphylaxis relevant cell hormone: lactobacillus reuteri (Lactobacillus reuteri) GL-104 bacterial strain, Chinese Typical Representative culture collection center preserving number M 209138; And
Pharmaceutically acceptable vehicle.
6. medical composition as claimed in claim 5, wherein this lactic bacterium strains is for having the bacterial strain of activity or deactivation (inactivated).
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| US9587284B2 (en) * | 2014-02-21 | 2017-03-07 | Asian Probiotics and Prebiotics Ltd. | Lactic acid bacterium having immunomodulatory and anti-allergic effects and pharmaceutical composition containing the same |
| CN107050063A (en) * | 2016-11-08 | 2017-08-18 | 江西益盟科技有限公司 | Treat lactic bacteria composition of allergic constitution and preparation method thereof |
| CN108338305A (en) * | 2017-01-22 | 2018-07-31 | 上海法太实业有限公司 | A kind of compound powder solid beverage of probiotics |
| TWI639389B (en) * | 2017-01-24 | 2018-11-01 | 豐華生物科技股份有限公司 | Food, oral cleaning and pharmaceutical composition with strains of lactic acid bacteria for inhibiting of oral pathogens |
| CN107114794A (en) * | 2017-06-06 | 2017-09-01 | 上海真合生物技术有限公司 | Probiotic composition for strengthening antiallergy ability |
| TWI709374B (en) * | 2019-06-14 | 2020-11-11 | 豐華生物科技股份有限公司 | Use of food composition and pharmaceutical composition with strains of lactic acid bacteria for modulating blood glucose |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101328468A (en) * | 2007-06-21 | 2008-12-24 | 东宇生物科技股份有限公司 | Antiallergic lactic acid bacteria |
| CN101575582A (en) * | 2008-05-08 | 2009-11-11 | 景岳生物科技股份有限公司 | Lactobacillus separation strains with anti-inflammatory activity and application thereof |
-
2010
- 2010-07-01 CN CN2010102267377A patent/CN102309001B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101328468A (en) * | 2007-06-21 | 2008-12-24 | 东宇生物科技股份有限公司 | Antiallergic lactic acid bacteria |
| CN101575582A (en) * | 2008-05-08 | 2009-11-11 | 景岳生物科技股份有限公司 | Lactobacillus separation strains with anti-inflammatory activity and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| John I.B.Alak,et al..Effect of Lactobacillus reuteri on Intestinal Resistance to Cryptosporidium parvum Infection in a Murine Model of Acquired Immunodeficiency Syndrome.《The Journal of Infectious Diseases》.1997,第175卷(第1期),218-221. * |
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