CN102305852B - Coloring agent for in vitro diagnostic device - Google Patents
Coloring agent for in vitro diagnostic device Download PDFInfo
- Publication number
- CN102305852B CN102305852B CN201110127782.1A CN201110127782A CN102305852B CN 102305852 B CN102305852 B CN 102305852B CN 201110127782 A CN201110127782 A CN 201110127782A CN 102305852 B CN102305852 B CN 102305852B
- Authority
- CN
- China
- Prior art keywords
- coloring agent
- mbth
- dyestuff
- diagnostic device
- vitro diagnostic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention relates to a coloring agent for an in vitro diagnostic device, in particular to a pigment generated through chemical oxidoreduction reaction, which can be used for quantitatively detecting biochemical substances in blood. A precursor of the coloring agent is easily combined with aromatic amines or aromatic alcohols, and the stable pigment is generated in hydrogen peroxide and oxygen environments, and cannot be interfered by protoheme in specific wavelength ranges.
Description
Technical field
The present invention relates to a kind of in-vitro diagnosis medical equipment coloring agent used (3-ethanol-6-methylbenzothiazole quinoline-(2)-hydrazone) synthetic method (EMBH), a kind of pigment particularly producing after chemistry redox reaction, can be in order to the biochemical in quantitative detection blood.
Background technology
Different types of redox organic dyestuff has been applied in the clinical diagnosis of hospital.But be not suitable for being used in small-sized or personal medical equipment at the conventional dyestuff great majority of hospital laboratory.Only have the dyestuff of minority special formulation successfully to use in individual medical equipment.
Generally, the electron accepter mixing formula of the precursor of dyestuff or dyestuff and plysiochemical material pairing to be measured, for example to use glucose oxidase be electron accepter to blood glucose measurement.Blood sugar and glucose oxidase and oxygen, after electron exchange, produce hydrogen peroxide.The precursor of the further oxidation dye of hydrogen peroxide and horseradish peroxidase or dyestuff.Consequent dye strength is directly proportional to plysiochemical material to be measured.
The most frequently used method of individual's diagnostic medical equipment is to use two kinds of dyestuffs.The advantage of this method is that the composition ratio fork-like farm tool used in ancient China of these two kinds of dyestuffs of capable of regulating regulates the reinforcement chromatogenous weight of institute and stability, the most frequently used a kind of dyestuff is 3-methyl-2-benzothiazolinone hydrazone hydrochloride hydrate (3-methyl-2-benzothiazolinone hydrazone hydrochloride hydrate at present, MBTH). this application is in extensive range, can be oxidized the dyestuff that produces different colours with various aromatic amines and alcohol coupling.
The people such as Phillips.At United States Patent (USP) the 4734360th, use MBTH to mix to measure with DMAB dimethylaminobenzaldehyde (p-dimethylaminobenzaldehyde) content that blood is dredged glucose in liquid in 5059394 and 5304468.Because MBTH easily distils, at high temperature MBTH distils and reduces content, has changed initial dyestuff ratio.In neutrality or alkaline pH value, MBTH can be precipitated out in aqueous solution, so be not suitable for being applied in the solution of high pH value.For example, the content of measuring Blood Cholesterol should carry out in pH8 left and right, in this pH value, does not have dyestuff to share chromogenesis with MBTH.
The people such as Yu, at U.S. Patent number 5453360, have delivered the synthetic method of a new MBTH derivant, and this MBTH derivant is higher and comparatively stable in high temperature in the solubleness of acid and alkaline aqueous solution than MBTH.But this MBTH derivant to light sensitive, is easy to oxidized and degrades when solid-state powdery.In addition, little molecule organic solvent, as acetone, can remain in crystal, causes disturbing the coupling reaction of dyestuff.
Nikolyukin etc.No. 5710012nd, United States Patent (USP).To deliver the 6th of a phenyl ring at MBTH dyestuff and added a carboxylic group. this derivant has good thermal stability.But its solubleness is very low 4 to 5 of pH values, is not suitable for blood glucose measurement.
The people such as Douglas, the sulfonic group derivant of delivering a MBTH No. 5776719 at United States Patent (USP), this derivant has good thermal stability and solubleness, but because sulfonic group has powerful electrophilic feature, the pigment of generation is shifted to red-light spectrum.Thereby by the protoheme interference measurement results in blood. in addition, this derivant has too high water-soluble, has influence on the unstable of measurement result.
The invention provides a kind of fuel system, in any pH value, have good water-solublely, there is good thermal stability, easily produce bluely to glaucous pigment with various aromatic amines and alcohol, not disturbed by protoheme.
Summary of the invention
One know-why:
The main difficulty that extracorporeal blood detects is that protoheme disturbs, the stability of medicament and solubleness, fuel system of the present invention adopts 3-ethanol-6-methylbenzothiazole quinoline-(2)-hydrazone (EMBH) and an oxidase and peroxidase, these three kinds of main agents are through special formulation, with conventional cementing agent, sequestrant, buffering agent is mixed into a kind of detection of drugs dyestuff of blood cause of disease, there is solubleness widely, high-temperature stability, and not disturbed by protoheme.
Two synthetic methods:
1) 2-amino-3-ethanol-6-methylbenzothiazole.
At 100 milliliters of round-bottomed flasks, add 10 grams of 2-amino-6-methylbenzothiazoles, 20 milliliters of methyl cellosolves, and 5 milliliters of ethylene iodohydrins.Slowly be heated to 85 degree, stir 6 hours simultaneously, allow its cool to room temperature, filter and approximately 5 grams of products that air-dry rear acquisition is desirable.
2) 3 ethanol-6-methylbenzothiazole quinoline-(2)-hydrazone (EMBH).
By 10 grams of 2-amino-3-ethanol-6-methylbenzothiazoles, the distilled water of 20 milliliters, the potpourri of 1 milliliter of hydrazine is heated to 60 degree 1 hour, after the rear pale yellow solution producing of filtration is cooling, obtains the yellow powder of 5 grams.
The synthetic method of 3-ethanol-6-methylbenzothiazole quinoline-(2)-hydrazone (EMBH) is shown in accompanying drawing 1.
Accompanying drawing 2 is absorption spectrum comparisons that EMBH and MBTH produce, and the absorption peak of protoheme is in 550nm left and right, and with the absorption juxtaposition of MBTH generation, but the absorption spectrum producing with EMBH is separated far away.
Three, technical indicator:
The spectral absorption peak of EMBH between 600-620nm,
The mass spectrophotometry molecular weight of EMBH is between 206-208
The ultimate analysis of EMBH is C10H13N3S
Accompanying drawing explanation
The synthetic method of accompanying drawing 1:3-ethanol-6-methylbenzothiazole quinoline-(2)-hydrazone (EMBH)
The absorption spectrum comparison that accompanying drawing 2:EMBH and MBTH and DMAB (dimethylaminobenzaldehyde) oxidation coupling produces.
Embodiment
Nylon membrane nearly 1.2 micro-not average pore sizes is evenly coated biology enzyme, is placed on 50 degrees Celsius of oven for drying 10 minutes, then coats dyestuff, according to above-mentioned same program, dries.Be cut into 0.5 square inch, be clipped in the mylar that two 0.005 inchs have 0.25 square inch of opening, a glucose solution is dropped on nylon membrane, then by catoptrics view, measure.The absorption peak of EMBH is at 607nm, and the absorption peak of MBTH is at 596nm.
Claims (2)
1. chemical reagent is used for measuring the purposes of physiological analytes of the biological fluids of human body or animal, described biological fluids comprises blood, serum and urine, described physiological analytes comprises blood sugar, cholesterol and triglyceride, and this chemical reagent comprises 3 ethanol-6-methylbenzothiazole quinoline-(2)-hydrazone.
2. chemical reagent is used for measuring the purposes of physiological analytes of the biological fluids of human body or animal, described biological fluids comprises blood, serum and urine, described physiological analytes comprises blood sugar, cholesterol and triglyceride, this chemical reagent comprises 3 ethanol-6-methylbenzothiazole quinoline-(2)-hydrazone, the formula combination of oxidase and/or peroxidase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110127782.1A CN102305852B (en) | 2011-05-17 | 2011-05-17 | Coloring agent for in vitro diagnostic device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110127782.1A CN102305852B (en) | 2011-05-17 | 2011-05-17 | Coloring agent for in vitro diagnostic device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102305852A CN102305852A (en) | 2012-01-04 |
| CN102305852B true CN102305852B (en) | 2014-12-10 |
Family
ID=45379733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110127782.1A Expired - Fee Related CN102305852B (en) | 2011-05-17 | 2011-05-17 | Coloring agent for in vitro diagnostic device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102305852B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108181470B (en) * | 2017-09-09 | 2020-05-26 | 广东医科大学 | Kit and method for detecting contents of endotoxin and (1-3) - β -D-glucan in jaundice blood sample |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0763396B2 (en) * | 1991-10-30 | 1995-07-12 | 丸善石油化学株式会社 | Reagent for detection of labeled peroxidase for immunoblot analysis |
| JP3342077B2 (en) * | 1992-02-03 | 2002-11-05 | ライフスキヤン・インコーポレーテツド | Improved oxidative coupling dyes for spectrophotometric determination of analytes |
| US5710012A (en) * | 1993-08-25 | 1998-01-20 | Actimed Laboratories, Inc. | Salt of 6-carboxy-3-methylbenzothiazolone hydrazone hydrate in colorimetric determination of hydrogen peroxide |
| US5776719A (en) * | 1997-07-07 | 1998-07-07 | Mercury Diagnostics, Inc. | Diagnostic compositions and devices utilizing same |
-
2011
- 2011-05-17 CN CN201110127782.1A patent/CN102305852B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102305852A (en) | 2012-01-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2288906T3 (en) | COLORING COUPLE FOR THE SPECTROPHOTOMETRIC DETERMINATION OF ANALYTES. | |
| Song et al. | An AIE-active fluorescence turn-on bioprobe mediated by hydrogen-bonding interaction for highly sensitive detection of hydrogen peroxide and glucose | |
| CN102020308B (en) | Nano copper oxide analogue enzyme and method for measuring hydrogen peroxide by using nano copper oxide analogue enzyme as peroxide analogue enzyme | |
| CN103483872B (en) | For the coloring composition and preparation method thereof of tumor tissue cell | |
| CN102507543A (en) | Method for enhancing luminol chemoluminescence using copper oxide nanoparticles | |
| JPS59166865A (en) | Composition detecting activity of peroxidase | |
| CN110243794A (en) | A kind of fluorescence probe for detecting sulfur dioxide and its application based on graphene quantum dot | |
| CN103808551A (en) | Diagnosis reagent for detecting pathological cells of epithelial tissue and preparation technology thereof as well as cotton swab | |
| Jarrett et al. | Assessment of mitochondrial damage in retinal cells and tissues using quantitative polymerase chain reaction for mitochondrial DNA damage and extracellular flux assay for mitochondrial respiration activity | |
| CN102305852B (en) | Coloring agent for in vitro diagnostic device | |
| JP2003528623A (en) | Reagent system for detecting the presence of reduced cofactor in a sample and methods of use thereof | |
| CN114570936B (en) | Preparation method of glutathione-S-transferase-gold-platinum nanocluster and application of glutathione-S-transferase-gold-platinum nanocluster in aureomycin detection | |
| AU2018100445A4 (en) | A method for dopamine detection with GO-PtCu nano-enzyme | |
| CN105572065B (en) | Glucose determination reagent box based on platinum bismuth nanometer Mimetic enzyme | |
| CN111141909A (en) | Method for detecting bacterial endotoxin in thioglycerol by gel method | |
| CN111253934B (en) | Two-photon fluorescent probe and preparation and application thereof | |
| CN104330393A (en) | Method for determining glucose by using gold nano-cluster as fluorescence probe | |
| CN108069413A (en) | A kind of method for preparing the double transmitting carbon dotss of red-green glow and application | |
| Zhang et al. | Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites | |
| CN113549449A (en) | Nano fluorescent probe, preparation method and application thereof | |
| JPS59162899A (en) | Method and composition for determination of beta-hydroxybutyric acid | |
| CN108148887A (en) | Coloring composition for detecting microorganism infection and preparation method thereof | |
| Wendeberg | Fluorescence in situ hybridization for the identification of environmental microbes | |
| CN108676554A (en) | A kind of composite Nano probe and preparation method thereof and application | |
| CN109342341A (en) | A method of utilizing carbon quantum dot Visual retrieval concentration of hydrogen peroxide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Huzhou Maidinuohua Medical Technology Co.,Ltd. Document name: Notification of Termination of Patent Right |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141210 Termination date: 20170517 |