CN102305759A - Fluid sheathing apparatus used in blood cell analysis - Google Patents
Fluid sheathing apparatus used in blood cell analysis Download PDFInfo
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- CN102305759A CN102305759A CN201110243613A CN201110243613A CN102305759A CN 102305759 A CN102305759 A CN 102305759A CN 201110243613 A CN201110243613 A CN 201110243613A CN 201110243613 A CN201110243613 A CN 201110243613A CN 102305759 A CN102305759 A CN 102305759A
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Abstract
The invention discloses a fluid sheathing apparatus used in blood cell analysis. The apparatus comprises a fluid sheathing housing. A first fluid sheathing chamber and a second fluid sheathing chamber are arranged in the fluid sheathing housing. Each fluid sheathing chamber comprises a fluid focusing section. One side of the fluid focusing section of each fluid sheathing chamber is provided with a sample inlet and two sheath fluid inlets which are axial-symmetrically arranged relative to the sample inlet central axis. The other side of the fluid focusing section of each fluid sheathing chamber is provided with a sheath fluid outlet. The sheath fluid outlet of the first fluid sheathing chamber is communicated with the sample inlet of the second fluid sheathing chamber. The central axes of the sample inlet of the first fluid sheathing chamber, the sheath fluid outlet of the first fluid sheathing chamber and the sheath fluid outlet of the second fluid sheathing chamber are positioned on a same straight line. With the apparatus provided by the invention, it is ensured that blood cells are linearly delivered through a detection zone on by one. According to the invention, after fluid sheathing, the flow rate of the blood cells is high, cell refluxing can be effectively reduced, the blood cells can be rapidly delivered through the detection zone, and a sample detecting speed is improved.
Description
Technical field
The present invention relates to a kind of medicine equipment, particularly a kind of blood cell analysis flows device with sheath.
Background technology
Blood middle leukocytes can be divided into five types according to the difference of volume and content; Be lymphocyte, monocyte, eosinophil, basophilic granulocyte, neutrophil cell, these five kinds of leucocytes are all detected needs to use five classification blood analyzers.
In five classification blood analyzers; For identifying five kinds of leucocytes; Need make the cell marshalling and pass through laser irradiation area one by one by laser radiation, according to signals such as detecting scattered light, fluorescence these five kinds of leukocyte differentiations opened, this method is called " flow cytometry ".
For guaranteeing that cell passes through laser irradiation area one by one; Need use sheath stream technology; Promptly the blood after the dilution gets into the sheath flow of rapid movement from sample mouth low speed in the streaming passage, through sheath fluid sample is focused on and quickens, and cell is clocklike detected through laser irradiation area one by one.
Blood cell analysis all is individual layer sheath stream chamber with sheath stream device at present; Sheath stream chamber has fluid focus portion; One side of fluid focus portion has sample inlet and two sheath fluid inlets that are provided with sample inlet central shaft rotational symmetry, and the opposite side of fluid collection portion has a sheath flow export.This sheath stream device haemocyte is slow through detection zone speed, also is easy to generate cell and refluxes.
Summary of the invention
The problem that the present invention will solve is: provide a kind of blood cell analysis to flow device with sheath, make it can improve the flowing velocity after the haemocyte sheath flows.
In order to solve the problems of the technologies described above; The present invention includes sheath stream housing; Be provided with first sheath stream chamber and second sheath stream chamber in the sheath stream housing; Two sheath stream chambers all have fluid focus portion; One side of the fluid focus portion of each sheath stream chamber has a sample inlet and two sheath fluid inlets that are provided with sample inlet central shaft rotational symmetry; The opposite side of the fluid collection portion of each sheath stream chamber has a sheath flow export; The sheath flow export of first sheath stream chamber communicates with the sample inlet of second sheath stream chamber, the sample inlet of first sheath stream chamber; The sheath flow export of first sheath stream chamber; The sheath flow export three's of second sheath stream chamber central axis point-blank.
Be convenient to clean in order to make the present invention; Described sheath stream housing comprises first sheath stream housing and second sheath stream housing; The inner chamber of first sheath stream housing is first sheath stream chamber; The inner chamber of second sheath stream housing is second sheath stream chamber; The main body of first sheath stream housing and second sheath stream housing is taper type; The large end face of second sheath stream housing is provided with the taper slot of an indent; First sheath stream housing is inserted in the slot of second sheath stream housing; The sample inlet of first sheath stream chamber and two sheath fluid inlets are arranged on the large end face of first sheath stream housing; The sheath flow export of first sheath stream chamber is arranged on the small end face of first sheath stream housing; Two sheath fluid inlets of second sheath stream chamber are arranged on the large end face of second sheath stream housing, and the sheath flow export of second sheath stream chamber is arranged on the small end face of second sheath stream housing.
For the ease of sample introduction; The large end face of described first sheath stream housing is provided with the taper slot of an indent; Be provided with sampling device in the slot of first sheath stream housing; The main body of sampling device also is a taper type; Establish injection port on the large end face of sampling device; Establish outlet on the small end face, the sampling device outlet is the sample inlet of first sheath stream chamber.
During beneficial effect of the present invention: the present invention can guarantee that haemocyte linearly passes through detection zone one by one; And compare sheath and flow device with single sheath stream chamber; Flowing velocity behind the haemocyte sheath stream of the present invention is fast; And can effectively reduce cell refluxes; Make haemocyte to pass through detection zone fast, improved the speed of pattern detection.
Description of drawings
Fig. 1 is a complete section structural representation of the present invention;
Among the figure: 1, second sheath stream housing, 2, the sheath fluid inlet, 3, the sheath fluid inlet, 4, sampling device, 5, slot; 6, sheath fluid inlet, 7, slot, 8, the sheath fluid inlet, 9, first sheath stream chamber; 10, first sheath stream housing, 11, the sheath flow export, 12, second sheath stream chamber, 13, the sheath flow export.
Embodiment
A kind of blood cell analysis as shown in Figure 1 flows device with sheath; Comprise sheath stream housing; Be provided with first sheath stream chamber 9 and second sheath stream chamber 12 in the sheath stream housing; Two sheath stream chambers are equipped with a sample inlet, two sheath fluid inlet and sheath flow exports that are provided with sample inlet central shaft rotational symmetry; The sheath flow export 11 of first sheath stream chamber 9 is connected with the sample inlet of second sheath stream chamber 12, and sheath flow export 13 threes' of the sample inlet of first sheath stream chamber 9, sheath flow export 11, second sheath stream chamber 12 central axis point-blank.
Sheath stream housing comprises first sheath stream housing 10, second sheath stream housing 1 and sampling device 4; The inner chamber of first sheath stream housing 10 is first sheath stream chamber 9; The inner chamber of second sheath stream housing 1 is second sheath stream chamber 12; The main body of first sheath stream housing 10 and second sheath stream housing 1 is taper type; The large end face of first sheath stream housing 10 is provided with the taper slot 5 of an indent; The large end face of second sheath stream housing 1 is provided with the taper slot 7 of an indent; The main body of sampling device 4 also is a taper type; Establish injection port on the large end face of sampling device 4; Establish outlet on the small end face; Sampling device 4 is inserted in the slot 5 of first sheath stream housing 10; The outlet of sampling device 4 is the sample inlet of first sheath stream chamber 9; The sheath fluid inlet 3 of first sheath stream chamber 9 is arranged on the large end face of first sheath stream housing 10 with sheath fluid inlet 6; The sheath flow export 11 of first sheath stream chamber 9 is arranged on the small end face of first sheath stream housing 10; The sheath fluid inlet 2 of second sheath stream chamber 12 is arranged on the large end face of second sheath stream housing 1 with sheath fluid inlet 8; The sheath flow export 13 of second sheath stream chamber 12 is arranged on the small end face of second sheath stream housing 1; First sheath stream housing 10 is inserted in the slot 7 of second sheath stream housing 1, and the sheath flow export 11 that makes the sheath of winning flow chamber 9 enters the mouth to be communicated with the sample of second sheath stream chamber 12 and is integral.
Principle of work: cell sample gets into sampling device 4 from injection port; Through pyramidal structure it being focused on the back gets into first sheath stream chamber 9 from outlet; The sheath fluid pair cell sample that flows into first sheath stream chamber 9 from sheath fluid inlet 3 and sheath fluid inlet 6 focuses on acceleration, and cell sample quickens the back through the focusing of sheath fluid and gets into second sheath stream chamber 12.Again from the sheath fluid of second sheaths stream chambers 12 that sheath fluid inlet 2 and sheath fluid inlet 8 advance once more the pair cell sample focus on acceleration, cell sample quickens the back through the focusing of sheath fluid and gets into second sheath stream chamber 12.Cell sample quickens the back through the secondary focusing of sheath fluid and detects from sheath flow export 13 entering optical detection zones.
Claims (3)
1. a blood cell analysis flows device with sheath; It is characterized in that: comprise sheath stream housing; Be provided with first sheath stream chamber (9) and second sheath stream chamber (12) in the sheath stream housing; Two sheath stream chambers all have fluid focus portion; One side of the fluid focus portion of each sheath stream chamber has a sample inlet and two sheath fluid inlets that are provided with sample inlet central shaft rotational symmetry; The opposite side of the fluid collection portion of each sheath stream chamber has a sheath flow export; The sheath flow export (11) of first sheath stream chamber (9) communicates with the sample inlet of second sheath stream chamber (12), the sample inlet of first sheath stream chamber (9); The sheath flow export (11) of first sheath stream chamber (9); Sheath flow export (13) three's of second sheath stream chamber (12) central axis point-blank.
2. blood cell analysis according to claim 1 flows device with sheath; It is characterized in that: described sheath stream housing comprises first sheath stream housing (10) and second sheath stream housing (1); The inner chamber of first sheath stream housing (10) is first sheath stream chamber (9); The inner chamber of second sheath stream housing (1) is second sheath stream chamber (12); The main body of first sheath stream housing (10) and second sheath stream housing (1) is taper type; The large end face of second sheath stream housing (1) is provided with the taper slot (7) of an indent; First sheath stream housing (10) is inserted in the slot (7) of second sheath stream housing (1); Sample inlet and two sheath fluid inlets (3,6) of first sheath stream chamber (9) are arranged on the large end face of first sheath stream housing (10); The sheath flow export (11) of first sheath stream chamber (9) is arranged on the small end face of first sheath stream housing (10); Two sheath fluid inlets (2,8) of second sheath stream chamber (12) are arranged on the large end face of second sheath stream housing (1), and the sheath flow export (13) of second sheath stream chamber (12) is arranged on the small end face of second sheath stream housing (1).
3. blood cell analysis according to claim 2 flows device with sheath; It is characterized in that: the large end face of described first sheath stream housing (10) is provided with the taper slot (5) of an indent; Be provided with sampling device (4) in the slot (5) of first sheath stream housing (10); The main body of sampling device (4) also is a taper type; Establish injection port on the large end face of sampling device (4); Establish outlet on the small end face, sampling device (4) outlet is the sample inlet of first sheath stream chamber (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110243613A CN102305759A (en) | 2011-08-24 | 2011-08-24 | Fluid sheathing apparatus used in blood cell analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110243613A CN102305759A (en) | 2011-08-24 | 2011-08-24 | Fluid sheathing apparatus used in blood cell analysis |
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| Publication Number | Publication Date |
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| CN102305759A true CN102305759A (en) | 2012-01-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201110243613A Pending CN102305759A (en) | 2011-08-24 | 2011-08-24 | Fluid sheathing apparatus used in blood cell analysis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106706479A (en) * | 2016-12-29 | 2017-05-24 | 桂林优利特医疗电子有限公司 | Particle sheath flow imaging device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63259466A (en) * | 1987-04-16 | 1988-10-26 | Hitachi Ltd | Cell analyzer |
| US5150037A (en) * | 1990-11-30 | 1992-09-22 | Toa Medical Electronics Co., Ltd. | Particle detector and particle detecting apparatus having the detector |
| CN1886315A (en) * | 2003-10-30 | 2006-12-27 | 赛托诺姆公司 | Multilayer hydrodynamic sheath flow structure |
| JP2011145185A (en) * | 2010-01-15 | 2011-07-28 | Sony Corp | Flow-channel structure, microchip, and solution sending method |
| CN202166598U (en) * | 2011-08-24 | 2012-03-14 | 山东兰桥医学科技有限公司 | Sheath flow device for blood corpuscle analysis |
-
2011
- 2011-08-24 CN CN201110243613A patent/CN102305759A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63259466A (en) * | 1987-04-16 | 1988-10-26 | Hitachi Ltd | Cell analyzer |
| US5150037A (en) * | 1990-11-30 | 1992-09-22 | Toa Medical Electronics Co., Ltd. | Particle detector and particle detecting apparatus having the detector |
| CN1886315A (en) * | 2003-10-30 | 2006-12-27 | 赛托诺姆公司 | Multilayer hydrodynamic sheath flow structure |
| JP2011145185A (en) * | 2010-01-15 | 2011-07-28 | Sony Corp | Flow-channel structure, microchip, and solution sending method |
| CN202166598U (en) * | 2011-08-24 | 2012-03-14 | 山东兰桥医学科技有限公司 | Sheath flow device for blood corpuscle analysis |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106706479A (en) * | 2016-12-29 | 2017-05-24 | 桂林优利特医疗电子有限公司 | Particle sheath flow imaging device |
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Application publication date: 20120104 |