CN102304077A - Method for purifying tryptophan - Google Patents
Method for purifying tryptophan Download PDFInfo
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- CN102304077A CN102304077A CN201110172018A CN201110172018A CN102304077A CN 102304077 A CN102304077 A CN 102304077A CN 201110172018 A CN201110172018 A CN 201110172018A CN 201110172018 A CN201110172018 A CN 201110172018A CN 102304077 A CN102304077 A CN 102304077A
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- alcoholic solution
- temperature
- purification
- tryptophane
- crude product
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- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The invention provides a method for purifying tryptophan. The method is characterized by comprising the following steps of: adding water into a crude product, increasing the temperature, dissolving, adding active carbon, decolourizing at high temperature, and filtering; decreasing the temperature, cooling and crystallizing filtrate; adding an alcoholic solution to rinse and filter after centrifuging; and dissolving and removing many alcohol soluble impurities, and obtaining the tryptophan after drying. According to the method for purifying the tryptophan provided by the invention, the crude product is filtered by using the GH87 active carbon and rinsed by using the alcoholic solution, therefore, the purity of the product is more than or equal to 96%; the light transmittance of a sample is also beyond the international standard; and the quality of the product is steady.
Description
Technical field
The present invention relates to a kind of method of purification of tryptophane, belong to medicine and make the field.
Background technology
Tryptophane is the important precursor substance of growth hormone biosynthesizing in the plant materials, and tryptophane requires its transmittance >=95% on international standard.But on present market, the transmittance of most tryptophane is lower, unstable product quality, and the time of laying, long slightly sample will turn to be yellow.
Summary of the invention
In order to overcome the shortcoming that prior art exists, the invention provides a kind of method of purification of tryptophane, it is characterized in that: this method of purification comprises:
1) crude product is added the water rising temperature for dissolving, and add active carbon high-temp decolouring and filtration;
2) filtrating cooling cooling, crystallization;
3) centrifugal back adds alcoholic solution rinsing filtration;
4) dissolve and to remove a large amount of alcohol dissolubility impurities, dry tryptophane.
The said temperature that adds entry is 70-100 ℃.
Said gac is that model is the gac of GH87.
Filtrating cooling refrigerative temperature is 3-5 ℃.
The alcoholic solution of said adding is 0-5 a times of said crude product amount, and said alcoholic solution is methyl alcohol or ethanol or propyl alcohol.
The method of purification of a kind of tryptophane provided by the invention; It is that the gac of GH87 filters crude product that this method has been used a kind of model, and carries out rinsing with alcoholic solution, makes product gas purity >=96%; And the transmittance of sample has also surpassed international standard, constant product quality.
Embodiment
Below be to further specify technical scheme of the present invention through embodiment.
Embodiment 1
1000 gram crude products are put into reaction kettle; Be dissolved in water and be warming up to 70 ℃; Toward the interior adding of reaction kettle model is the gac of GH87; Being heated to 70 ℃ decolours, filters; The cooling of will filtrating again is cooled to 3 ℃; Centrifugal back adds 3000 gram ethanol carries out rinsing and filters, and dissolves and removes a large amount of alcoholic solution impurity, dry again product.Through detecting, the purity of tryptophane is 97%, and transmittance is 96%.
Embodiment 2
500 gram crude products are put into reaction kettle; Be dissolved in water and be warming up to 90 ℃; Toward the interior adding of reaction kettle model is the gac of GH87; Being heated to 100 ℃ decolours, filters; The cooling of will filtrating again is cooled to 5 ℃; Centrifugal back adds 2500 gram methyl alcohol carries out rinsing and filters, and dissolves and removes a large amount of alcoholic solution impurity, dry again product.Through detecting, the purity of tryptophane is 98%, and transmittance is 98%.
Claims (5)
1. the method for purification of a tryptophane, it is characterized in that: this method of purification comprises:
1) crude product is added the water rising temperature for dissolving, and add active carbon high-temp decolouring and filtration;
2) filtrating cooling cooling, crystallization;
3) centrifugal back adds alcoholic solution rinsing filtration;
4) dissolve and to remove a large amount of alcohol dissolubility impurities, dry tryptophane.
2. method of purification according to claim 1 is characterized in that: the said temperature that adds entry is 70-100 ℃.
3. method of purification according to claim 1 is characterized in that: said gac is that model is the gac of GH87.
4. method of purification according to claim 1 is characterized in that: filtrating cooling refrigerative temperature is 3-5 ℃.
5. method of purification according to claim 1 is characterized in that: the alcoholic solution of said adding is 0-5 a times of said crude product amount, and said alcoholic solution is methyl alcohol or ethanol or propyl alcohol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110172018A CN102304077A (en) | 2011-06-24 | 2011-06-24 | Method for purifying tryptophan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110172018A CN102304077A (en) | 2011-06-24 | 2011-06-24 | Method for purifying tryptophan |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102304077A true CN102304077A (en) | 2012-01-04 |
Family
ID=45378016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110172018A Pending CN102304077A (en) | 2011-06-24 | 2011-06-24 | Method for purifying tryptophan |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102304077A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830945A (en) * | 2015-05-15 | 2015-08-12 | 南通荣泰生物科技有限公司 | Production process of D-tryptophan |
| CN104829519A (en) * | 2015-05-15 | 2015-08-12 | 南通荣泰生物科技有限公司 | Purification process of L-tryptophan |
| CN105061289A (en) * | 2015-07-28 | 2015-11-18 | 蚌埠丰原医药科技发展有限公司 | Preparation method of pharmaceutical grade L-tryptophan |
| CN111410627A (en) * | 2020-04-03 | 2020-07-14 | 石家庄市冀荣药业有限公司 | production method of pharmaceutical-grade L-tryptophan |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61126070A (en) * | 1984-11-21 | 1986-06-13 | Mitsui Toatsu Chem Inc | Method of decoloring and purifying l-tryptophan |
| JPS63177796A (en) * | 1987-01-14 | 1988-07-21 | Ajinomoto Co Inc | Purification of tryptophan |
| US5057615A (en) * | 1989-06-27 | 1991-10-15 | Mitsui Toatsu Chamicals, Inc. | Process for purifying tryptophan |
| CN101245047A (en) * | 2008-03-04 | 2008-08-20 | 汕头市紫光古汉氨基酸有限公司 | Purification method for tryptophane |
-
2011
- 2011-06-24 CN CN201110172018A patent/CN102304077A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61126070A (en) * | 1984-11-21 | 1986-06-13 | Mitsui Toatsu Chem Inc | Method of decoloring and purifying l-tryptophan |
| JPS63177796A (en) * | 1987-01-14 | 1988-07-21 | Ajinomoto Co Inc | Purification of tryptophan |
| US5057615A (en) * | 1989-06-27 | 1991-10-15 | Mitsui Toatsu Chamicals, Inc. | Process for purifying tryptophan |
| CN101245047A (en) * | 2008-03-04 | 2008-08-20 | 汕头市紫光古汉氨基酸有限公司 | Purification method for tryptophane |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830945A (en) * | 2015-05-15 | 2015-08-12 | 南通荣泰生物科技有限公司 | Production process of D-tryptophan |
| CN104829519A (en) * | 2015-05-15 | 2015-08-12 | 南通荣泰生物科技有限公司 | Purification process of L-tryptophan |
| CN105061289A (en) * | 2015-07-28 | 2015-11-18 | 蚌埠丰原医药科技发展有限公司 | Preparation method of pharmaceutical grade L-tryptophan |
| CN105061289B (en) * | 2015-07-28 | 2017-11-17 | 蚌埠丰原医药科技发展有限公司 | A kind of method for preparing pharmaceutical grade L tryptophans |
| CN111410627A (en) * | 2020-04-03 | 2020-07-14 | 石家庄市冀荣药业有限公司 | production method of pharmaceutical-grade L-tryptophan |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120104 |