CN102283280A - Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases - Google Patents
Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases Download PDFInfo
- Publication number
- CN102283280A CN102283280A CN201110166697A CN201110166697A CN102283280A CN 102283280 A CN102283280 A CN 102283280A CN 201110166697 A CN201110166697 A CN 201110166697A CN 201110166697 A CN201110166697 A CN 201110166697A CN 102283280 A CN102283280 A CN 102283280A
- Authority
- CN
- China
- Prior art keywords
- fruit
- rhodotorula mucilaginosa
- fruits
- phytic acid
- rhodotorula
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Storage Of Fruits Or Vegetables (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases. The method comprises the following steps of: activating the rhodotorula mucilaginosa, inoculating the activated rhodotorula mucilaginosa to an NYDB culture medium, culturing, and performing centrifugation to obtain bacteria; diluting the bacteria by using phytic acid solution to prepare 1*10<8> bacteria per milliliter bacteria suspension; putting fruits into the bacteria suspension, soaking the fruits for 30 seconds, immediately taking out the fruits, and naturally airing the fruits; and putting the fruits into a plastic basket, sealing the fruits by using a preservative film, and storing the fruits at room temperature or under a refrigerating condition. The method enhances the prevention and control effect of the rhodotorula mucilaginosa on the fruit postharvest diseases by using the phytic acid, and is simple in use, convenient for operation, good in effect and low in cost. The rhodotorula mucilaginosa with enhanced prevention and control effect can substitute a chemical bactericide to prevent and control the fruit postharvest diseases, so that harm of the chemical bactericide to the human body is avoided; and the method has remarkable economic benefit and social benefit.
Description
Technical field
The present invention relates to utilize phytic acid (phytic acid) strengthen the rhodotorula mucilaginosa bacterial classification (
Rhodotorula mucilaginosa) to the method for fruit postharvest diseases preventing efficiency, belong to fruit and adopt the artifact prevention and control field.
Background technology
Fruit because of the loss of rotting to cause is huge, has become the problem that the whole world is paid close attention in harvesting, transportation and sales, storage.It is reported that the loss that developed country causes thus is 10%-25%, then up to 40%-50%, it is about 20%-25% that China's fruit is adopted the back loss in the developing country that lacks cold storage establishment.In March, 2007, State Statistics Bureau's issue " People's Republic of China's national economy and social development statistical communique in 2006 " showed that China's fruit total output reached 17,050 ten thousand tons in 2006.Estimation in view of the above, the loss after China's fruit is adopted will reach tens billion of yuans level every year.
It is the main cause that fruit is adopted the back loss that pathogenic microorganism infects rotting of causing, these pathogenic microorganisms mainly contain Botrytis cinerea (
Botrytis cinerea), Rhizopus stolonifer (
Rhizopus stoloniger), mould (
Penicillium spp.), Mucor (
Mucor spp.), chain lattice spore (
Alternaria spp.) and aspergillus (
Aspergillus spp.) etc.Pathogenic microorganism to adopt the back fruit harm not only be the heavy losses that it causes fruit quantitatively to cause, thereby and since many disease funguses can secrete the generation many secondary metabolites cause serious food-safety problem, as penicillium expansum (
P. expansum) produce clavacin (patulin), Botrytis cinerea (
B. cinerea) botrydial (botrydial) that produces etc.
The method of rotting after the most frequently used control fruit is adopted at present is that cryopreservation and chemical bactericide are handled.The cryopreservation technology has obtained developing widely and using in China in recent years, but existing refrigerating equipment often can not satisfy the needs of most fruits.Simultaneously, under cryogenic conditions, still have a considerable amount of pathogenic bacteria on fruit, to grow, and cause fruit to rot.And those can only be preserved being not less than under the temperature that product is damaged to plants caused by sudden drop in temperature the fruit of low-temperature sensitive.
For a long time, the major measure of control fruit postharvest diseases is to use bactericide.Chemical bactericide can play the effect of getting instant result to preventing and treating disease, and still, long-term and a large amount of uses not only causes pathogenic microorganism to develop immunity to drugs, and serious environment pollution impairs human health simultaneously.The portion report of NAS (National Academy of Scinces) shows that at the whole agricultural chemicals that are used for handling food, bactericide constitutes 60% tumorigenesis danger.
Raising along with the national economic development and the level of consumption; people are to the enhancing day by day of food security and environmental protection consciousness; number of chemical bactericide (as Captan, Benomyl etc.) has been prohibited and has been used or restriction use on the part fruit product at present, and its direct consequence is that present many fruit postharvest diseases have not had bactericide to be controlled.Therefore, the various countries scientist all explore energetically can replace chemical bactericide safe, the novel fruits postharvest disease is prevented and treated method efficiently.Currently in the world the research of the biological control of carrying out fruit postharvest diseases with antagonistic microbe has been obtained interim achievement, and thought that this is one of method that is hopeful most by the substituted chemistry bactericide.
Along with yeast classification, ecology, physiology, biochemistry and molecular biological research make progress, people improve day by day to the interest of utilizing yeast in the fruit vegetables storing.Domestic and international research shows that many saccharomycete are applied to fruit surface, can prevent and treat the disease that fruit is caused by pathogenic moulds, and these yeast are called as antagonism yeast.The saccharomycete inheritance stability, antimicrobial spectrum is wide, the height of tiring, not general the generation to people and the harmful metabolite of host plant, safe, and low to nutritional requirement, growth is fast, and to multiplely coerce, adverse circumstance has stronger tolerance, insensitive to most of bactericide, can be compatible to other chemistry and physical treatment.Therefore antagonism yeast is subjected to international extensive concern as the fruit postharvest diseases biocontrol microorganisms in recent years.
China's biologically controlling postharvest disease of fruit research is started late.Fan Qing etc. (2001) screen fruit disease biological control antagonism bacterium and separate, and the result shows: monilia guilliermondii (
Candida guiliermondii), lemon shape Ke Leke yeast (
Kloeckera apiculata), the inferior Dbaly yeast of the Chinese (
Debaryomyces hansenii) the sweet cherry brown rot is all shown significant inhibition effect, monilia guilliermondii and lemon shape Ke Leke yeast can be prevented and treated drupe class fruit soft rot effectively; Field generation equality (2004) research sprays 1 * 10 before sweet cherry is adopted
8The trichosporon cutaneum of individual/mL (
Trichosporon pullulans), Cryptococcus laurentii (
Cryptococcus laurentii) and rhodotorula glutinis (
Rhodotorula glutinis), respectively at 25 ℃, under 0 ℃ and the air conditioned storage, trichosporon cutaneum is bred quantity on fruit the highest, and fungistatic effect is best; Zhang Hongyin etc. (2005) have studied under the different temperatures, and Cryptococcus laurentii is to the prevention effect of gray mold, and the result shows, under 25 ℃, the incidence of disease of pears gray mold is 16.7%, in conjunction with CaCl
2Result of use is more remarkable.
The current as can be known control that is used for fruit postharvest diseases with effective microbe substituted chemistry bactericide has demonstrated great application prospect from domestic and foreign literature, and the biological control of fruit postharvest diseases becomes a new research focus.It is few to be applied to the antagonism bacterial classification class of producing, have only a few launch such as Biosave and Aspire, and China does not have still at present the antagonism bacterium to be applied to actual production but up to the present.Main cause is under the commodity production condition that the prevention effect of the antagonistic microbe of having reported at present often is not so good as the effect of chemical bactericide, thereby does not reach fresh-keeping requirement.These factor affecting the antagonism bacterium as fruit and vegetable fresh-keeping agent use aborning.Rhodotorula mucilaginosa is that we screen a strain antagonism yeast that is separated at seminar from the soil in the nuisanceless orchard of middle bar, Zhenjiang.We studies show that in a large number: rhodotorula mucilaginosa has significant inhibitory effect to the postharvest disease of fruit such as strawberry, peach.Yet our result of the test shows also that rhodotorula mucilaginosa is compared with chemical bactericide the preventing efficiency of fruit disease and still has bigger gap.Therefore further improve the antagonism of rhodotorula mucilaginosa and render a service, be to improve it, and be applied to one of fresh-keeping Critical policies of fruit storage fruit postharvest diseases control level.
Phytic acid is a feedstock production with corn, rice bran or wheat.As a kind of biodegradable natural materials, phytic acid is aboundresources not only, and cheap, non-toxic and safe.Because of it has numerous unique biological characteristics, extensive studies and application are all arranged in fields such as food and fruit post-harvest fresh-keepings.In fruit post-harvest fresh-keeping field, phytic acid is used as desirable fruit coating, is used for ascorbic degraded in the delayed fruit, keeps soluble solid and acid content in the fruit.Studies show that phytic acid to the microbial rotten inhibition effect of multiple cause of disease a little less than, so phytic acid must be used with other anticorrisive agent when using as antistaling agent.
In recent years, how the non-bactericide method with phytic acid and other safety effectively integrates, with a research focus that obtains that fruit is adopted the higher levels of control of back fungal disease, become to be paid close attention to very much in the world.Adopt back antagonism yeast biological control and render a service and still do not have relevant report but both at home and abroad can phytic acid be cultivated to influence.
Summary of the invention
The present invention provides a kind of method that the rhodotorula mucilaginosa biological control is renderd a service that strengthens in order to overcome above-mentioned deficiency of the prior art.Rhodotorula mucilaginosa dilutes through plant acid solution, is prepared into bacteria suspension.Bacteria suspension is applied on the fruit, can suppresses the fruit pathogen significantly, prevent to rot after fruit from adopting.The present invention has safety, efficient, low cost and other advantages, can be widely used in the biological control process of fruit postharvest diseases, reduces the loss that fruit postharvest diseases causes.
The present invention provides a kind of phytic acid that utilizes to strengthen rhodotorula mucilaginosa to the fruit postharvest diseases preventing efficiency simultaneously, thereby is applied to the fresh-keeping method of fruit storage.Carry out according to following step: with rhodotorula mucilaginosa (
Rhodotorula mucilaginosa) activation, be inoculated in the NYDB culture medium and cultivate, the centrifugal thalline that obtains; Thalline is prepared into 1 * 10 with sterilized water and plant acid solution dilution
8The bacteria suspension of individual/mL; Fruit is put into bacteria suspension, soak after 30 seconds and take out at once, natural air drying; Be placed in the plastic crate, after the preservative film sealing, be placed under room temperature or the refrigerated condition and deposit.
Yeast extract 5g in the wherein said NYDB culture medium, beef extract 8g, glucose 10g, purified water 1000ml, natural pH.
The mass concentration of phytic acid is 0.05-0.5% in the wherein said bacteria suspension.
Wherein said rhodotorula mucilaginosa (
Rhodotorula mucilaginosa), preservation strain is numbered CGMCC No.3617.
Wherein said fruit preferably apple, peach or strawberry.
Advantage of the present invention:The present invention uses phytic acid to strengthen the effectiveness of rhodotorula mucilaginosa control fruit postharvest diseases, uses simply, and easy to operate, effective, cost is low.Rhodotorula mucilaginosa after the enhancing can replace chemical bactericide control fruit postharvest diseases, avoids using the harm of chemical bactericide to human body, has remarkable economic efficiency and social benefit.
Description of drawings:
Wherein Fig. 1 is that the variable concentrations phytic acid combines the inhibition effect to grey mould fruit rot of strawberry with rhodotorula mucilaginosa; Annotate: CK: contrast; 0:NYDB; 0.1%-0.5% is the bacteria suspension after the dilution of 0.1%-0.5% plant acid solution.The incidence of disease is 20 ℃ and places the result who measures after 3 days.The significance of difference (p=0.05) represented in different letters.
Fig. 2 is that the variable concentrations phytic acid combines the inhibition effect to strawberry head mold disease with rhodotorula mucilaginosa; Annotate: CK: contrast; 0:NYDB; 0.1%-0.5%: the bacteria suspension after the dilution of 0.1%-0.5% plant acid solution.The incidence of disease is 20 ℃ and places the result who measures after 3 days.The significance of difference (p=0.05) represented in different letters.
Fig. 3 is that the variable concentrations phytic acid combines the inhibition effect to the peach penicilliosis with rhodotorula mucilaginosa; Annotate: CK: contrast; 0:NYDB; 0.0.5%-0.25%: the bacteria suspension after the dilution of 0.05%-0.25% plant acid solution.The incidence of disease is 20 ℃ and places the result who measures after 6 days.The significance of difference (p=0.05) represented in different letters.
Fig. 4 is that the variable concentrations phytic acid combines the inhibition effect to the peach gray mold with rhodotorula mucilaginosa; Annotate: CK: contrast; 0:NYDB; 0.0.5%-0.25%: the bacteria suspension after the dilution of 0.05%-0.25% plant acid solution.The incidence of disease is 20 ℃ and places the result who measures after 6 days.The significance of difference (p=0.05) represented in different letters.
Fig. 5 is that the variable concentrations phytic acid combines the inhibition effect to peach head mold disease with rhodotorula mucilaginosa; Annotate: CK: contrast; 0:NYDB; 0.0.5%-0.25%: the bacteria suspension after the dilution of 0.05%-0.25% plant acid solution.The incidence of disease is 20 ℃ and places the result who measures after 4 days.The significance of difference (p=0.05) represented in different letters.
Fig. 6 is that the variable concentrations phytic acid combines the inhibition effect to green mold of apple with rhodotorula mucilaginosa; Annotate: CK: contrast; 0:NYDB; 0.1%-0.5%: the bacteria suspension after the dilution of 0.1%-0.5% plant acid solution.The incidence of disease is 20 ℃ and places the result who measures after 8 days.The significance of difference (p=0.05) represented in different letters.
Fig. 7 is that the variable concentrations phytic acid combines the inhibition effect notes to the apple gray mold with rhodotorula mucilaginosa: CK: contrast; 0:NYDB; 0.1%-0.5%: the bacteria suspension after the dilution of 0.1%-0.5% plant acid solution.The incidence of disease is 20 ℃ and places the result who measures after 10 days.The significance of difference (p=0.05) represented in different letters.
The specific embodiment
By by following embodiment with more detailed explanation the present invention.Following examples only are illustrative, and the present invention is not subjected to the restriction of these embodiments.
Rhodotorula mucilaginosa is that this laboratory screening separation obtains, now be stored in the Institute of Microorganism, Academia Sinica of No. 3, the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City that is positioned at the BeiJing, China, China Committee for Culture Collection of Microorganisms's common micro-organisms revenue centre (CGMCC), preservation strain is numbered CGMCC No.3617, adds 2% agar on the basis of NYDA(NYDB culture medium) 4 ℃ of low temperature preservations of culture medium.The cultivation program is: the activation of (1) solid: rhodotorula mucilaginosa is inoculated in the NYDA culture medium, cultivates 48h at 28 ℃; (2) Liquid Culture: the NYDB seed culture medium of 50 ml that in the triangular flask of 250m1, pack into, insert the good rhodotorula mucilaginosa of two ring activation with oese, 200 rpm (rev/min), cultivate 20h under 28 ℃ of conditions; (3) strengthen: with centrifugal 10min under above-mentioned culture mix 7000 * g condition, the SPSS washed twice, removing culture medium, and with the SPSS yeast cells that suspends again, it is 5 * 10 that blood cell counting plate is regulated cell concentration
8Individual/mL.In the triangular flask of 250m1, be respectively charged into 50ml NYDB culture medium, and add the yeast saccharomyces nutrient solution 1ml of above-mentioned concentration,, cultivate 24h under 28 ℃ of conditions then at 200 rpm; (4) centrifugation suspends again: above-mentioned different disposal Yeast Cultivation mixture under 7000 * g condition, centrifugal 10min, and,, be diluted to desired concn with SPSS and plant acid solution again to remove culture medium with SPSS washing 2 times.Use therein phytic acid is available from Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.
Embodiment 1: phytic acid strengthens the inhibition effect of rhodotorula mucilaginosa to the fruit disease
One, testing program
After fruit is handled, form the wound of the unified size and the degree of depth at each position, fruit surface equator with sterile card punch.Wherein the superficial cut of strawberry is the 3mm(diameter) * 3mm(is dark), the superficial cut of peach fruit is the 5mm(diameter) * 3mm(is dark), the superficial cut of apple is the 5mm(diameter) * 3mm(is dark).Each wound equivalent adds 30 μ l(1) 1 * 10
8Individual/ml is through the yeast cream of SPSS dilution; (2) 1 * 10
8Individual/ml is through the yeast cream (containing the phytic acid mass concentration is 0.1%-0.5%) of phytic acid dilution; (3) sterilized water (in contrast).Behind the 3h, add the pathogen spore suspension of equivalent 30 μ l respectively in each wound.Wherein strawberry adds 1 * 10 respectively
5The Botrytis cinerea of individual/ml (
Botrytis cinerea) spore suspension and 1 * 10
4The Rhizopus stolonifer of individual/ml (
Rhizopus stoloniger) spore suspension; The peach fruit adds 1 * 10 respectively
5The Botrytis cinerea of individual/ml (
Botrytis cinerea) spore suspension, 1 * 10
4The Rhizopus stolonifer of individual/ml (
Rhizopus stoloniger) spore suspension and 5 * 10
4The penicillium expansum of individual/ml (
Penicillium expansum) spore suspension; Apple adds 1 * 10 respectively
5The Botrytis cinerea of individual/ml (
Botrytis cinerea) spore suspension and 5 * 10
4The penicillium expansum of individual/ml (
Penicillium expansum) spore suspension.Naturally after drying, fruit is put into plastic crate and with preservative film sealing, cultivate in the constant incubator (water activity 95%), strawberry places 20 ℃, and peach fruit and apple place 25 ℃.
Every processing repeats 3 times, and each repeats 18 fruits.Whole test repeats 2 times.Through some days cultivation, calculate the incidence of disease, with this estimate the rhodotorula mucilaginosa bacterial classification (
Rhodotorula mucilaginosa) fungistatic effect.
The computing formula of the incidence of disease is as follows:
Fruit sum * 100% of the fruit number/inoculation pathogen of the incidence of disease (%)=morbidity
Two, result of the test
According to the above-mentioned steps test, the rotting rate result of back statistics fruit is as follows:
1.1 inhibition effect to grey mould fruit rot of strawberry and head mold disease
As can be seen from Figure 1, after rhodotorula mucilaginosa was handled, the incidence of disease of grey mould fruit rot of strawberry all was markedly inferior to the incidence of disease of contrast strawberry.The phytic acid of rhodotorula mucilaginosa and 0.1%, 0.2% and 0.3% concentration is used in combination, and it significantly strengthens the inhibitory action that infects of Botrytis cinerea in the strawberry wound.Especially when phytic acid concentration was 0.2%, the incidence of disease of its gray mold only was 28.3% of contrast behind the saccharomycete suspension processing strawberry, handled 51.5% of the back strawberry incidence of disease for saccharomycete uses (not combining with phytic acid) separately.Similar result has also appearred to rhodotorula mucilaginosa in the inhibitory action test of infecting in the strawberry wound to Rhizopus stolonifer as can be seen from Figure 2.
1.2
Inhibition effect to peach fruit penicilliosis, gray mold and head mold disease
As shown in Figure 3, rhodotorula mucilaginosa is used in combination with 0.15% phytic acid, and its inhibition that peach fruit wound penicilliosis is infected is renderd a service significantly and strengthened.Mainly show the whole test stage, combine the peach fruit of handling with 0.15% phytic acid, the peach fruit that its penicilliosis incidence significantly is lower than contrast and handles separately through rhodotorula mucilaginosa through rhodotorula mucilaginosa.Similar result (as shown in Figure 4 and Figure 5) has also appearred on the inhibitory action to peach fruit wound gray mold and head mold disease.
Inhibition effect to green mold of apple and gray mold
As shown in Figure 6 and Figure 7, result of the test shows that concentration is that 0.2% phytic acid can significantly improve rhodotorula mucilaginosa the control of green mold of apple, gray mold is renderd a service, and only is 41%, 35% of contrast.
Above result shows, phytic acid can strengthen rhodotorula mucilaginosa to Botrytis cinerea (
Botrytis cinerea), Rhizopus stolonifer (
Rhizopus stoloniger), mould (
Penicillium spp.) prevention effect of the gray mold, penicilliosis and the head mold disease that cause.
Embodiment 2:Phytic acid strengthens rhodotorula mucilaginosa rots to reach storage quality naturally to fruit influence
One, testing program
Behind the test fruit picking directly with following processing immersion fruit 30s:(1) the rhodotorula mucilaginosa suspension of NYDB medium culture; (2) yeast cream of plant acid solution dilution; (3) sterilized water (in contrast).Then fruit is placed in the plastic crate, air-dry back seals with preservative film.Strawberry preserves in 4 ℃ of refrigerator-freezers, changes 20 ℃ of insulating box storages after 20 days over to, observes the fruit rot situation after 5 days, calculates rotting rate; Peach really preserves in 4 ℃ of refrigerator-freezers, changes 25 ℃ of incubator storages after 30 days over to, observes the fruit rot situation after 7 days, calculates rotting rate, and carries out the storage quality analysis; Apple is preserved in 4 ℃ of refrigerator-freezers, changes 25 ℃ of incubator storages after 30 days over to, observes the fruit rot situation after 10 days, calculates rotting rate, and carries out the storage quality analysis.Every processing repeats 3 times, repeats 10 fruits at every turn, and whole test repeats 2 times.
The storage quality analytical method is as follows:
(1) hardness: adopt the test of TA-XT2i texture analyser, probe diameter is 5mm, and the speed of service before the probe test, in the test, after the test is respectively 4.0 mm s
1, 1.0 mm s
1, 5.0 mm s
1, the strawberry MTD is 6 mm, and peach fruit and apple MTD are 10 mm, and suffered maximum resistance (unit is newton) just was defined as the hardness of fruit when probe inserted fruit.Each fruit is located per 120 ° and is measured 3 times around the equator.
(2) total dissoluble solid: adopt WYT(0-80%) hand-held saccharometer is measured, and measurement result is expressed as the g/100g sample.
(3) mensuration of Vit C contents: ultraviolet rapid test method.Get 20 g pulp samples from 6 fruit and add 20 ml, 1% hydrochloric acid homogenate, 100 ml volumetric flasks are settled to scale.10000 * g, centrifugal 10 mim under 4 ℃ of conditions, supernatant are used for measuring.Get 0.2 ml extract, put into the 10 ml scale test tubes that fill 0.4 ml, 10% HCl, to scale, shake up with distilled water diluting.With distilled water is blank, measures absorbance at 243 nm places.Other draws 0.2 ml extract, adds 4 ml distilled water and 0.6 ml 1moll respectively
1NaOH solution to 10 ml shakes up to the scale test tube, leave standstill 15min after.Add 0.6 ml 10%HCl, mixing, adding distil water is settled to scale.With distilled water is blank, measures absorbance at 243 nm places.A by liquid to be measured and alkali treatment liquid to be measured
243What be worth is poor, looks into calibration curve, ascorbic content in the calculation sample.Every processing repeats for 3 times.
(4) brown stain degree: 6 fruit take by weighing the 20g pulp organization, add 40 ml distilled water homogenate, 10000 * g, centrifugal 10 mim under 4 ℃ of conditions, get supernatant 10 ml and add 95% ethanol 15ml, 10000 * g, centrifugal 10 mim under 4 ℃ of conditions get supernatant and measure absorbance in 420 nm, represent browning degree with this.Every processing repeats for 3 times.
Two, result of the test
According to the above-mentioned steps test, statistics strawberry, peach fruit and the natural rotting rate of apple and the influence of storage quality.
The result is as follows:
Table 1 phytic acid combines the influence to strawberry nature rotting rate and storage quality with rhodotorula mucilaginosa
CK: contrast; 0: rhodotorula mucilaginosa; The plant acid solution of 0.2:0.2% is handled; The phytic acid of 0+0.2:0.2% combines processing with rhodotorula mucilaginosa.Change 20 ℃ of results that measure after placing 5 days at 4 ℃ over to after placing 20 days when the incidence of disease of strawberry and quality determination in the above-mentioned table.The significance of difference (p=0.05) represented in different letters.
As shown in table 1, rhodotorula mucilaginosa is with after 0.2% phytic acid is used in combination, and the inhibitory action that strawberry is rotted naturally is remarkable, and the rotting rate of strawberry only is 55.6% of contrast, and is markedly inferior to the rotting rate of the strawberry that the rhodotorula mucilaginosa that do not combine with phytic acid handles.Compare with the rhodotorula mucilaginosa in conjunction with phytic acid not, the rhodotorula mucilaginosa that strengthens through 0.2% phytic acid does not all produce adverse effect to hardness, soluble solid, titratable acid, Vc content, the brown stain degree of strawberry.
Table 2 phytic acid combines the influence of the really whole fruit of peach being rotted to reach naturally storage quality with rhodotorula mucilaginosa
CK: contrast; 0: rhodotorula mucilaginosa is handled; The plant acid solution of 0.15:0.15% is handled; The phytic acid of 0+0.15:0.15% combines processing with rhodotorula mucilaginosa.Change 20 ℃ of results that measure after placing 7 days after 30 days over to 4 ℃ of placements when the incidence of disease in the above-mentioned table and quality determination.The significance of difference (p=0.05) represented in different letters.
0.15% phytic acid is handled in conjunction with rhodotorula mucilaginosa, and the inhibitory action that the peach fruit is rotted naturally is remarkable, and the natural rotting rate of peach fruit of its processing is about 71.4% of the independent peach fruit rotting rate of handling of rhodotorula mucilaginosa.Simultaneously, combine the peach fruit of handling with rhodotorula mucilaginosa through 0.15% phytic acid, its hardness and soluble solid content are really compared with the contrast peach, no significant difference, its hardness and Vc content significantly increase, and the brown stain degree significantly reduces, and every index of quality is not all produced adverse effect (table 2).
Table 3 phytic acid combines influence rotten naturally to the whole fruit of apple and storage quality with rhodotorula mucilaginosa
CK: contrast; 0: rhodotorula mucilaginosa; The plant acid solution of 0.2:0.2% is handled; The phytic acid of 0+0.2:0.2% combines processing with rhodotorula mucilaginosa.Change 20 ℃ of results that measure after placing 10 days after 30 days over to 4 ℃ of placements when the incidence of disease in the above-mentioned table and quality determination.The significance of difference (p=0.05) represented in different letters.
0.2% phytic acid is in conjunction with rhodotorula mucilaginosa, and the apple of its processing nature rotting rate only is 57.1% of contrast, and is lower than the rotting rate that rhodotorula mucilaginosa uses the apple of processing separately.Simultaneously, combine the apple of handling with rhodotorula mucilaginosa through 0.2% phytic acid, its hardness is really compared with the contrast peach, no significant difference, soluble solid content and titratable acid content significantly increase, and the brown stain degree significantly reduces, and every index of quality is not all produced adverse effect (table 3).
Above result of the test shows that phytic acid can strengthen the inhibitory action of rhodotorula mucilaginosa to strawberry, peach fruit and apple nature disease of rotting, and the fruit storage quality is not had a negative impact.
Claims (5)
1. one kind is improved the method that rhodotorula mucilaginosa is renderd a service biologically controlling postharvest disease of fruit, it is characterized in that carrying out according to following step: with rhodotorula mucilaginosa (
Rhodotorula mucilaginosa) activation, be inoculated in the NYDB culture medium and cultivate, the centrifugal thalline that obtains; Thalline is prepared into 1 * 10 with the plant acid solution dilution
8The bacteria suspension of individual/mL; Fruit is put into bacteria suspension, soak after 30 seconds and take out at once, natural air drying; Be placed in the plastic crate, after the preservative film sealing, be placed under room temperature or the refrigerated condition and deposit.
2. a kind of method that rhodotorula mucilaginosa is renderd a service biologically controlling postharvest disease of fruit that improves according to claim 1 is characterized in that yeast extract 5g in the wherein said NYDB culture medium, beef extract 8g, glucose 10g, water 1000ml, natural pH.
3. a kind of method that rhodotorula mucilaginosa is renderd a service biologically controlling postharvest disease of fruit that improves according to claim 1, the mass concentration that it is characterized in that phytic acid in the wherein said bacteria suspension is 0.05-0.5%.
4. a kind of method that rhodotorula mucilaginosa is renderd a service biologically controlling postharvest disease of fruit that improves according to claim 1, it is characterized in that wherein said rhodotorula mucilaginosa (
Rhodotorula mucilaginosa), preservation strain is numbered CGMCC No.3617.
5. a kind of method that rhodotorula mucilaginosa is renderd a service biologically controlling postharvest disease of fruit that improves according to claim 1 is characterized in that wherein said fruit is apple, peach or strawberry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110166697A CN102283280A (en) | 2011-06-21 | 2011-06-21 | Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110166697A CN102283280A (en) | 2011-06-21 | 2011-06-21 | Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102283280A true CN102283280A (en) | 2011-12-21 |
Family
ID=45330260
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110166697A Pending CN102283280A (en) | 2011-06-21 | 2011-06-21 | Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102283280A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178432A (en) * | 2014-08-08 | 2014-12-03 | 成都医学院 | Rhodotorula mucilaginosa and application thereof |
| CN108414256A (en) * | 2018-01-15 | 2018-08-17 | 青岛海尔股份有限公司 | A kind of detection method of fridge freshness retaining performance |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101892167A (en) * | 2010-06-10 | 2010-11-24 | 江苏大学 | Rhodotorula mucilaginosa, application thereof in preventing and curing postharvest disease of fruit and use method thereof |
-
2011
- 2011-06-21 CN CN201110166697A patent/CN102283280A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101892167A (en) * | 2010-06-10 | 2010-11-24 | 江苏大学 | Rhodotorula mucilaginosa, application thereof in preventing and curing postharvest disease of fruit and use method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 《中国酿造》 20080331 丁筑红等 《植酸对几株真菌生长的研究》 第9-13页 1-5 , 第3期 * |
| 《农业工程学报》 20030731 张红印等 《酵母菌对果蔬采后病害防治的研究进展》 第23-27页 1-5 第19卷, 第4期 * |
| 贺立红等: "《拮抗酵母菌对指状青霉菌的抑菌作用》", 《仲恺农业工程学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104178432A (en) * | 2014-08-08 | 2014-12-03 | 成都医学院 | Rhodotorula mucilaginosa and application thereof |
| CN108414256A (en) * | 2018-01-15 | 2018-08-17 | 青岛海尔股份有限公司 | A kind of detection method of fridge freshness retaining performance |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101899400B (en) | Pichiacaribbica, application thereof in fruit storage and refreshment and use method thereof | |
| CN101914475B (en) | Lactobacillus used for biological preservation and application thereof | |
| CN103820343B (en) | Application that saccharomyces cerevisiae is prevented and treated at fruit postharvest diseases and using method | |
| CN103146586B (en) | Trichoderma harzianum strain for controlling plant fungus diseases and application thereof | |
| CN104694430B (en) | Bacillus amyloliquefaciens plant subspecies ZFH 3 and its application | |
| CN102283281A (en) | Method for improving biological control efficacy of pichia caribbica to fruit postharvest diseases | |
| CN107460134A (en) | The Pichia guilliermondii of one plant of control the operatic circle postharvest disease | |
| CN102827790B (en) | Bacillus subtilis strain A97, fungicide, and preparation method and application thereof | |
| CN101892167B (en) | Rhodotorula mucilaginosa, application thereof in preventing and curing postharvest disease of fruit and use method thereof | |
| CN101911968B (en) | Method for enhancing effect of rhodotorula glutinis on biologically controlling postharvest disease of fruit | |
| CN101697750A (en) | Method for preventing fruit postharvest diseases | |
| CN101914459B (en) | Peach fruit disease biological antiseptic preservative and application and used cryptococcus laurentii | |
| CN104017748B (en) | Endogeny biocontrol strain as well as preparation method and application of biocontrol bacterial agent prepared from endogeny biocontrol strain | |
| CN102246850B (en) | Biological preservative for oranges | |
| Zhang et al. | Burdock fructooligosaccharide enhances biocontrol of Rhodotorula mucilaginosa to postharvest decay of peaches | |
| CN105861340B (en) | Pichia anomala for controlling postharvest diseases of citrus and grapes | |
| CN102224842A (en) | Orange biological preservative based on antagonistic activities of three antagonistic yeasts | |
| CN112481141B (en) | Debaryomyces hansenii strain and application thereof in preparation of fruit preservative | |
| CN102388955A (en) | Method for processing disease control after strawberry is picked by combining ozone and Cryptococcus laurentii | |
| CN103125590A (en) | Grape biological fresh-keeping agent and preparation method thereof | |
| CN102197842B (en) | Method for controlling apple patulin | |
| CN102283280A (en) | Method for improving biological prevention and control effect of rhodotorula mucilaginosa on fruit postharvest diseases | |
| CN103988896A (en) | Prevention and treatment on postharvest diseases of fruits by using glycine betaine-induced culture of pichia caribbica | |
| CN103988893A (en) | Method for preventing and treating fruit postharvest diseases by using compound of ascorbic acid and Pichia caribbica | |
| CN103843880B (en) | Based on the biological preservation liquid and uses thereof of GABA in conjunction with bio-control yeast |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20111221 |