CN102274443B - Compound Chinese medicinal microemulsion preparation capable of improving immunity - Google Patents
Compound Chinese medicinal microemulsion preparation capable of improving immunity Download PDFInfo
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- CN102274443B CN102274443B CN 201110229080 CN201110229080A CN102274443B CN 102274443 B CN102274443 B CN 102274443B CN 201110229080 CN201110229080 CN 201110229080 CN 201110229080 A CN201110229080 A CN 201110229080A CN 102274443 B CN102274443 B CN 102274443B
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Abstract
The invention discloses a compound Chinese medicinal microemulsion preparation capable of improving immunity, which comprises the following components in percentage by weight: 1.8 to 10 percent of oil phase, 3.7 to 33.2 percent of surfactant, 0 to 8 percent of auxiliary surfactant and 51 to 90 percent of water phase, wherein the oil phase is a mixture of garlic oil and blumea oil; the surfactant may consist of one or several of Tween-80, ethoxylated hydrogenated castor oil and span-80; the auxiliary surfactant may consist of one or several of glycerol, ethanol, n-butyl alcohol and 1,3-butanediol; and the water phase is water solution of polysaccharide at a mass concentration of 0.5 to 15 percent, and the polysaccharide may consist of one or several of polysaccharide from portulaca oleracea, astragalus polysaccharides, dandelion polysaccharide and Chinese bulbul polysaccharide. The compound Chinese medicinal microemulsion preparation has the advantages of high bioavailability, low cost, simple preparation and the like and can be widely used in production process of animal breeding.
Description
Technical field
The present invention relates to a kind of compound Chinese medicinal microemulsion preparation, especially a kind of compound Chinese medicinal microemulsion preparation that can improve immunity.
Background technology
The development of and scale intensive along with animal husbandry, healthy aquaculture and food safety have caused people's extensive concern.Chinese herbal feed additive is in modernization, intensive farm are already produced, the product that performance Chinese medicine disease-prevention health, the superiority development such as promote grow, have no drug resistance get up is low with its toxicity, side effect is little, many target spots, pathogenic microorganism are difficult for the chemical sproof advantage of generation to it and cause that people more and more pay attention to.Chinese medicine immunity enhancer is playing an important role aspect immunosuppressive disease and the enhancing human body immunity effect.Chinese medicine can strengthen by different model of action the non-specific and specific immunity of animal body, as strengthening immunogenicity and the stability of macrophage activity, antigenic substance, the synthetic and secretion of enhancing antibody etc.Some can non-specifically change or the enhancement antigen material, and the mechanism of different its immunological enhancements of Chinese medicine ingredients also is not quite similar, and especially highlights on multi-target effect.Therefore, Chinese medicine immunity enhancer will have vast potential for future development in promoting the livestock birds health cultivation process.
In volatile oil ImmunopharmacologicaResearch research, the volatile oil in the Bulbus Allii---garlicin enjoys attention, and extensive use studies confirm that in aquaculture, and garlicin has significant immunological enhancement, can improve lysozyme content in the serum and the activity of some enzymes.The herbal polysaccharide research experiment proves that polysaccharide has the multiple biological activitys such as Promote immunity adjusting, antitumor, antiviral, radioprotective, defying age, parasiticide, and disease prevention and health care are had obvious promotion and potentiation.Simultaneously, polysaccharide is natural product, has that toxic and side effects is little, noresidue, does not produce the advantages such as drug resistance, thereby have broad application prospects.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of compound Chinese medicinal microemulsion preparation that can improve immunity of advantages such as having the bioavailability height, cost is low, preparation is simple.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
The compound Chinese medicinal microemulsion preparation that can improve immunity of the present invention, its weight consists of: oil phase 1.8-10%, surfactant 3.7-33.2%, cosurfactant 0-8%, water 51-90%;
Wherein, the mixture that described oil phase is comprised of Oleum Bulbus Allii and Oleum Folium Artemisiae Argyi, wherein Oleum Bulbus Allii is 50-70%, Oleum Folium Artemisiae Argyi is 30-50%;
Described surfactant is tween 80, polyoxyethylene hydrogenated Oleum Ricini, one or more in the span-80;
Described cosurfactant is one or more in glycerol, ethanol, n-butyl alcohol and the 1,3 butylene glycol;
Described water is that mass concentration is the 0.5-15% polysaccharide solution, and described polysaccharide is one or more in Polysaccharide from Portulaca oleracea, astragalus polysaccharides, Taraxacum Polysaccharides and the Radix Pulsatillae polysaccharide;
A kind of preparation method that improves the compound Chinese medicinal microemulsion preparation of immunity comprises the steps:
(1) surfactant and cosurfactant are mixed in proportion.
(2) Oleum Bulbus Allii and Oleum Folium Artemisiae Argyi are added in the mixed system of surfactant and cosurfactant, and stir.
(3) under the room temperature, in the mixture of step (2) gained, dropwise add while stirring polysaccharide solution, until system becomes sticky by rare, again by sticking thinning, the solution clear.
The microemulsion formulation of the present invention's preparation detects through the Ma Erwen Particle Size Analyzer, and particle diameter is at 10-100nm, and the outward appearance clarification is bright, good fluidity, steady quality.Below be by experiment physicochemical property, stability and the immunostimulant performance of microemulsion formulation to be detected:
Testing 1 microemulsion physicochemical property detects
(1) outward appearance of microemulsion
The microemulsion of preparation is brown color (being colourless when not adding polysaccharide solution), and clarification is transparent, good fluidity.
(2) form of microemulsion
The herbal mixture microemulsion that takes a morsel is an amount of, routinely the operation transmission electron microscope observation.The result shows that microemulsion great majority under Electronic Speculum are spheroidal.
(3) particle diameter of microemulsion
It is an amount of to get the herbal mixture microemulsion, and with measuring with the Ma Erwen Particle Size Analyzer behind an amount of distilled water diluting, measurement result shows that the mean diameter of the herbal mixture microemulsion of test preparation is 19.41.The coefficient of dispersion is 0.594, and as seen the particle size distribution of this microemulsion formulation is narrower, and size ratio is more even.
(4) type of microemulsion
Utilizing staining is the type that speed that oil-soluble dyes tonyred (redness) and water-soluble dye methylene blue (blueness) spread in microemulsion is judged microemulsion, if red diffusion then is the w/o type microemulsion faster than blueness; Otherwise then be O/W type microemulsion, the result shows that the microemulsion of the present invention's preparation is the O/W type.
Test 2 stability tests
(1) centrifugal acceleration test is on the impact of this traditional medicine volatile oil stability of microemulsion
In centrifuge tube, sealing orifice places high speed centrifuge, carries out centrifugal 20min with 13,000r/min in right amount in the present invention who gets preparation, and this microemulsion still keeps original outward appearance, and clear has no lamination.
(2) photo-stability testing is on the impact of this traditional medicine volatile oil stability of microemulsion
An amount of the present invention is prepared Chinese medicine micro emulsion place Clear glass bottles and jars, sealing places under the daylight lamp, and light at room temperature is according to 15d, and in 1d, 3d, 5d, 10d, the 15d observation of taking a sample, the result shows that the present invention at the duration of test apparent colour significant change does not occur, and without phenomenons such as layering and breakdowns of emulsion.
(3) the temperature stability test is on the impact of this traditional medicine volatile oil stability of microemulsion
The present invention of preparation is sub-packed in the vial in right amount, and sealing is placed on to keep sample under 4 ℃, 25 ℃, 37 ℃ and the 60 ℃ of conditions investigates 3 months, and sampling in per 15 days is observed.The result shows that the present invention remains the outward appearance of clear under these four kinds of temperature conditions, have no obvious layering and demulsifying phenomenon.
Test the test of 3 immunostimulant
Protective effect to chicken colibacillosis
240 of 21 Day-old Broiler Chickens are divided into 4 groups at random, every group of 3 repetitions.Processing mode and result of the test are as follows:
Wherein the blank group after administration the 5th day intramuscular injection normal saline 0.2mL/ only, model group and microemulsion group intramuscular injection escherichia coli O1 bacterial strain 10
8CFU 0.2mL/ only.。
The grouping of table 1 animal and result of the test
| Group | Medication | Dead number of elements | Mortality rate (%) |
| The blank group | —— | 0 | 0 |
| Model control group | —— | 33 | 55 |
| Herbal mixture microemulsion low dose group | 50mL microemulsion+950mL diseases caused by retention of fluid water | 30 | 50 |
| Herbal mixture microemulsion high dose group | 150mL microemulsion+850mL diseases caused by retention of fluid water | 10 | 16.67 |
By experimental result as can be known, the mortality rate of herbal mixture microemulsion high dose group has reduced by 33.33 percentage points than model control group.Herbal mixture microemulsion low dose group mortality rate is suitable with model group.
We also find the difference of a chicken death time in experimentation, result such as table 2:
The death time statistical result of table 2 laboratory animal
| Group | 0h-24h | 24h-36h | 36h-48h | 48h-72h | 72h-96h |
| The blank group | 0 | 0 | 0 | 0 | 0 |
| Model control group | 4 | 6 | 10 | 8 | 5 |
| Herbal mixture microemulsion low dose group | 1 | 2 | 8 | 10 | 9 |
| Herbal mixture microemulsion high dose group | 0 | 1 | 3 | 5 | 1 |
As shown in Table 2, morbidity and the death time of herbal mixture microemulsion group chicken are more late than the chicken peak mortality time of occurrence of model group, and this has just won the time for the treatment of disease.
The prepared compound Chinese medicinal microemulsion preparation technique of the present invention is simple, has good stability, and uses safety, and production cost is low, long shelf-life.Can significantly strengthen the resistance against diseases of animal, can be widely used in the animal cultivation production process.
The beneficial effect that adopts technique scheme to produce is:
1. the prepared microemulsion bioavailability of the present invention is higher, can effectively reduce the use amount of medicine, and this microemulsion uses traditional medicine volatile oil effect oil phase, does not need to select independent oil phase, greatly reduces the cost of microemulsion preparation.
2. effectively solve Oleum Bulbus Allii and the Oleum Folium Artemisiae Argyi dissolubility is low, the resting period is short, the shortcoming such as easily go mouldy.
3. traditional medicine volatile oil and the immiscible problem of polysaccharide solution in the solution practical operation make both perfect adaptations, bring into play clinically dual immunological enhancement.
4. this microemulsion formulation in use can dilute with water, forms the microemulsion of clear, and this microemulsion Thermodynamically stable is easy to preparation and stores.
5. prescription and the method simple possible of the present invention's employing are convenient to large-scale industrial production.
The specific embodiment
Embodiment 1
1) oil phase: Oleum Bulbus Allii 0.9g, Oleum Folium Artemisiae Argyi 0.9g;
Surfactant: tween 80 10g;
Water: mass concentration is 10% astragalus polysaccharides aqueous solution 88.2g.
2) with surfactant and the cosurfactant mix homogeneously selected, then oil phase is added in the mixed system of surfactant and cosurfactant, and stir.
3) under the room temperature, dropwise add while stirring polysaccharide solution in the mixture of step (2) gained, until system is become sticky by rare, by sticking thinning, the solution clear namely gets particle diameter at the compound Chinese medicinal microemulsion preparation of 10-100 nm again.
Embodiment 2
1) oil phase: Oleum Bulbus Allii 2g, Oleum Folium Artemisiae Argyi 1g;
Surfactant: tween 80 6g;
Cosurfactant: ethanol 4g, glycerol 3g, n-butyl alcohol 3g;
Water: mass concentration is 0.5% Polysaccharide from Portulaca oleracea aqueous solution 40g, and mass concentration is 2% Taraxacum Polysaccharides aqueous solution 50g.
2) with surfactant and the cosurfactant mix homogeneously selected, then oil phase is added in the mixed system of surfactant and cosurfactant, and stir.
3) under the room temperature, dropwise add while stirring polysaccharide solution in the mixture of step (2) gained, until system is become sticky by rare, by sticking thinning, the solution clear namely gets particle diameter at the compound Chinese medicinal microemulsion preparation of 10-100 nm again.
Embodiment 3
1) oil phase: Oleum Bulbus Allii 3g, Oleum Folium Artemisiae Argyi 2g;
Surfactant: polyoxyethylene hydrogenated Oleum Ricini 20g, span-80 10g;
Cosurfactant: 1,3 butylene glycol 4g;
Water: mass concentration is 15% Taraxacum Polysaccharides aqueous solution 71g.
2) with surfactant and the cosurfactant mix homogeneously selected, then oil phase is added in the mixed system of surfactant and cosurfactant, and stir.
3) under the room temperature, dropwise add while stirring polysaccharide solution in the mixture of step (2) gained, until system is become sticky by rare, by sticking thinning, the solution clear namely gets particle diameter at the compound Chinese medicinal microemulsion preparation of 10-100 nm again.
Embodiment 4
1) oil phase: Oleum Bulbus Allii 4.2g, Oleum Folium Artemisiae Argyi 2.8g;
Surfactant: tween 80 25g;
Cosurfactant: n-butyl alcohol 8g;
Water: mass concentration is 7% Radix Pulsatillae polysaccharide solution 60g.
2) with surfactant and the cosurfactant mix homogeneously selected, then oil phase is added in the mixed system of surfactant and cosurfactant, and stir.
3) under the room temperature, dropwise add while stirring polysaccharide solution in the mixture of step (2) gained, until system is become sticky by rare, by sticking thinning, the solution clear namely gets particle diameter at the compound Chinese medicinal microemulsion preparation of 10-100 nm again.
Embodiment 5
1) oil phase: Oleum Bulbus Allii 5g, Oleum Folium Artemisiae Argyi 4g;
Surfactant: span-80 33.2g
Cosurfactant: 1,3 butanediol 5g;
Water: mass concentration is 5% astragalus polysaccharides aqueous solution 30g, and mass concentration is 10% Polysaccharide from Portulaca oleracea aqueous solution 22.8g.
2) with surfactant and the cosurfactant mix homogeneously selected, then oil phase is added in the mixed system of surfactant and cosurfactant, and stir.
3) under the room temperature, dropwise add while stirring polysaccharide solution in the mixture of step (2) gained, until system is become sticky by rare, by sticking thinning, the solution clear namely gets particle diameter at the compound Chinese medicinal microemulsion preparation of 10-100 nm again.
Embodiment 6
1) oil phase: Oleum Bulbus Allii 7g, Oleum Folium Artemisiae Argyi 3g;
Surfactant: tween 80 10g, polyoxyethylene hydrogenated Oleum Ricini 10g, span-80 12g;
Cosurfactant: glycerol 7g;
Water: mass concentration is 3% astragalus polysaccharides aqueous solution 51g.
2) with surfactant and the cosurfactant mix homogeneously selected, then oil phase is added in the mixed system of surfactant and cosurfactant, and stir.
3) under the room temperature, dropwise add while stirring polysaccharide solution in the mixture of step (2) gained, until system is become sticky by rare, by sticking thinning, the solution clear namely gets particle diameter at the compound Chinese medicinal microemulsion preparation of 10-100 nm again.
Claims (2)
1. the compound Chinese medicinal microemulsion preparation that can improve immunity is characterized in that the weight of described compound Chinese medicinal microemulsion preparation consists of: oil phase 1.8-10%, surfactant 3.7-33.2%, cosurfactant 0-8%, water 51-90%;
The mixture that described oil phase is comprised of Oleum Bulbus Allii and Oleum Folium Artemisiae Argyi; The mass percent of described oil phase consists of: Oleum Bulbus Allii 50-70%, Oleum Folium Artemisiae Argyi 30-50%;
Described surfactant is tween 80, polyoxyethylene hydrogenated Oleum Ricini, one or more in the span-80;
Described cosurfactant is one or more in glycerol, ethanol, n-butyl alcohol and the 1,3 butylene glycol;
Described water is that mass concentration is the 0.5-15% polysaccharide solution, and described polysaccharide is one or more in Polysaccharide from Portulaca oleracea, astragalus polysaccharides, Taraxacum Polysaccharides and the Radix Pulsatillae polysaccharide.
2. a kind of compound Chinese medicinal microemulsion preparation that can improve immunity according to claim 1, the particle diameter that it is characterized in that microemulsion formulation is 10-100nm.
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| CN104921005A (en) * | 2015-03-20 | 2015-09-23 | 徐州黎明食品有限公司 | Garlic oil nanometer-scale microemulsion liquid and preparation method thereof |
| US20210355374A1 (en) * | 2020-05-15 | 2021-11-18 | Saudi Arabian Oil Company | Enhancing foam stability using allium sativum oil |
| CN117322536A (en) * | 2023-10-31 | 2024-01-02 | 宁夏大洋饲料科技有限公司 | Premixed feed for improving calf organism immunity and preparation method thereof |
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