CN1022734C - Apparatus for separation of blood components - Google Patents

Abstract

The present invention relates to a device for separating blood components, which comprises a blood plasma container, a container for containing residual plasma in which refrigeration precipitates are taken out, and a container for containing a solution for dissolving the refrigeration precipitates, wherein the blood plasma container is connected with a blood plasma delivery pipe; the container for containing residual plasma in which refrigeration precipitates are taken out is connected with the blood plasma container by a conduit pipe; a container for containing a solution for dissolving the refrigeration precipitates is connected with the blood plasma container by a connecting gear. With the help of the device, refrigeration precipitates containing quite high concentration of blood coagulation factor VIII can be easily separated from blood plasma with high efficiency, and fibrinogen can be removed. Fibrinogen has a high content in common refrigeration precipitates and is frequently a reason for a secondary reaction when blood coagulation factor VIII is delivered in bulk.

Description

Apparatus for separation of blood components

The present invention relates to a kind of device that is used for separating blood constituents.Or rather, the present invention relates to a kind of device of coming such as this class blood constituent of platelet cofactor isolated effectively in the blood plasma.

Hemophilia is a kind of hematopathy of heredity, it is characterized in that because of platelet cofactor or the active a kind of nonvolatil bleeding tendency that presents of factor shortage.For want of factor VIII and the hemophilia that causes be haemophilia A (or factor, hemophilia vera), because of lacking hemophilia that factor IX causes haemophilia B (or factor, Christmas Day disease).Unfortunately, hemophilia does not also have the method for radical cure.The common Therapeutic Method that follows so far is need give haemophilia A patient recruitment factor VIII by situation, or gives haemophilia B patient recruitment factor IX.When hemophiliac was hemorrhage, the supply thrombin will be at the right time.The hemophilia patient has an operation or controls before the tooth, first supply thrombin.Factor VIII is unsettled, can loss of activity under stored condition.Therefore, the recruitment factor VIII must be used fresh blood.Factor IX is highly stable, the recruitment factor IX need only be with stored blood just.If utilize whole blood or blood plasma to replenish, the thrombin that obtain q.s is very difficult.Generally all adopt intravenous injection, such as freezing precipitation thing (Kong Shi first plasma protein), the spissated preparation of thrombinogen (being used for the recruitment factor IX) of perhaps spissated factor VIII (being used for the recruitment factor VIII) or purification or the like.

In the preparation factor VIII process, height enrichment factor VIII could obtain the concentrate formulation of factor VIII.Because the activity of factor VIII reduces in concentration process, the preparation that only is equivalent to 20% concentration after the injection works.In addition, prepare used raw material blood plasma and gather on one's body from many blood transfusion alms givers, the probability of sneaking into preparation such as viruses such as hepatitis viruss is very high.Otherwise the freezing precipitation thing is to use from the minority alms giver by a kind of simple method to gather the blood plasma preparation that comes on one's body, thereby it is lower to sneak into the probability of virus.And injection blood vessel postfactor VIII blood coagulation activity recovery rate height.Yet the preparation concentrating degree is lower, thereby liquid volume is bigger, and contains a lot of Fibrinogens and other unnecessary albumen.After a large amount of injections, may improve blood plasma global cycle amount, increase fibrinogen concentration, cause such as secondary reactions such as haemolysis.

Want to eliminate the shortcoming of common preparation method, importantly in the freezing precipitation thing, remove Fibrinogen effectively, guarantee again that simultaneously factor VIII do not lose activity.So far also do not realize effectively eliminating unnecessary proteic gratifying means.

Thereby one of purpose of the present invention provides a kind of new device that is used for separating blood constituents.

Another object of the present invention provides a kind of device of new separation platelet cofactor, and it is used for isolating unit that the freezing precipitation thing comes and one to one and is used for removing fibrinogenic unit combines from the freezing precipitation thing from blood plasma.

Another object of the present invention provides a kind of device that is used to obtain the low Factor of unnecessary protein content, and this is to rely on a kind of simple method, isolates platelet cofactor effectively from the blood plasma from minority blood transfusion alms giver.

Purpose described above is to realize that by a kind of device of separating blood constituents it comprises a blood plasma container, is connected with an end of a blood plasma carrier pipe; Comprise that also holds a container that withdraws residue blood plasma behind the freezing precipitation thing, the pipe of the residue blood plasma that withdraws the freezing precipitation thing that it is separated in the blood plasma container by a conveying, can anastomosing mode connect (maybe can connect) blood plasma container with a kind of: also comprise a container that holds the solvent that dissolving freezing precipitation thing uses that has bindiny mechanism, it can anastomosing mode connect (maybe can connect) blood plasma container by this bindiny mechanism with a kind of.

The invention still further relates to a kind of blood constituent segregation apparatus, it does bindiny mechanism with a conduit.Conduit one end has a pin, and the other end connects the container that holds freezing precipitation thing solvent.The present invention also is related to a kind of blood constituent segregation apparatus, and a path blockade mechanism that can set up circulation status is arranged in its bindiny mechanism.The invention reside in a kind of blood constituent segregation apparatus, broken parts are arranged in its path blockade mechanism, can fracture it, distribution channel is opened.The present invention also is a kind of blood component segregation apparatus.Its blood plasma container is carried by one with the residue blood plasma container and is remained interconnection being in the same place of conduit that blood plasma is used.The present invention also is a kind of device of separating blood composition.Its blood plasma container and residue blood plasma container.Can be interconnection by a conduit of carrying residue blood plasma to use.In addition, the objective of the invention is a kind of blood component segregation apparatus, it uses the solvent of neutral aqueous solution as dissolving freezing precipitation thing, this neutral aqueous solution contains at least and belongs to such as water-soluble amino acidses such as glycine, lysine, alanine, paddy acyl ammonia, such as saccharides such as sucrose, glucose, mannitols, and such as a kind of material of neutral salt apoplexy due to endogenous wind such as sodium chloride, potassium chloride.

The present invention also is a container, it has bindiny mechanism, be used for anastomosing mode to be connected with above-mentioned other container with a kind of, its the inside is holding the freezing precipitation thing solvent that is made of neutral aqueous solution, this neutral aqueous solution comprises a kind of material that belongs in water-soluble aminoacid, saccharide, the neutral salt three major types at least, as the stabilizing agent of factor.

This expression way is represented a kind of like this mode in this article " can anastomosing mode to connect (maybe can connect) with a kind of ": two containers are together interconnection by a conduit, be provided with path blockade mechanism in the distribution channel of conduit, can there be broken parts in this path blockade mechanism, and these broken parts that fracture just can be opened distribution channel.Also represent a kind of like this mode: two containers are together interconnection by a conduit, and an end of conduit is equipped with pin or thorn.

Fig. 1 is a front view as the various typical blood component segregation apparatus of the various combining forms of the present invention to Fig. 7.

Fig. 8 and Fig. 9 are sectional drawings, and diagram can be set up the typical path blockade mechanism of flow regime.

Figure 10 is the typical sectional view that mixes mouth.

Hereinafter with reference to corresponding diagram, the present invention is described in more detail.Fig. 1 a kind of typical blood component segregation apparatus that drawn is as one embodiment of the present invention.One end of blood plasma input pipe 1 connects blood plasma container 2.If necessary, a hollow needle 3 is housed on the other end of blood plasma input pipe 1.Hollow needle 3 outer masks medicated cap 4.Blood plasma container 2 is connected on the residue blood plasma container 7 via the pipe 6 of carrying the freezing precipitation thing to be withdrawn the remaining blood plasma in back, have on the residue blood plasma carrier pipe 6 can be anastomosing path blockade mechanism 5.Residue can be equipped with on the blood plasma container 7 some to mix mouthful, and the latter can use needle-penetration, reaches and the purpose of path is set up in the inside that remains blood plasma container 7.Be covered with the sealing member 8 and 9 that can sting on these mixing mouths.Blood plasma container 2 is connected with a freezing precipitation thing solvent container 12 via a conduit 11 of supplying with freezing precipitation thing solvent, this conduit 11 be have can be anastomosing the circulation mechanism of path blockade mechanism 10.Container holds freezing precipitation thing solvent for 12 li.Be connected on the container 12 have can be anastomosing the releasing tube 14 of path blockade mechanism 13.Releasing tube 14 front ends have the tapered coupling 15 of a protrusion.Adapter 15 outer masks medicated cap

When preparing to use, blood plasma container 2(is if necessary) be connected with blood vessel 19 via blood plasma input pipe 1, have a mixing mouth that is covered with the sealing member 17 that can sting on the blood vessel 19, and a blood input pipe 18.Blood input pipe 18 front ends are connected to hollow needle 20.Hollow needle 20 outer masks medicated cap 21.

Fig. 2 provides another embodiment of the invention.In blood component segregation apparatus shown in Figure 1, increased again one have can the anastomosing path blockade 17a of mechanism freezing precipitation thing solvent supply pipe 18a, be connected on the blood plasma container 2.Pipe 18a front end has a hollow needle 22, and latter's outer mask medicated cap 23.Container 26 is normally empty.In Fig. 2 and Fig. 1, all the same label numerals are the parts of TYP all, in the embodiment shown in Figure 2, if do not need solvent container repeatedly to repeat to hold freezing precipitation thing solution, then needn't limit the shape of solvent container specially.For example, can adopt the shape of injection tube or bottle.

Fig. 3 provides another embodiment of the invention.In this embodiment, mix mouth on the freezing precipitation thing solvent container 12, container was just filled freezing precipitation thing solvent originally.18b represents the platelet cofactor releasing tube in the figure.18b pipe front end is an outstanding adapter 28 then, and outer mask medicated cap 29.In Fig. 3 and Fig. 2, all identical label numerals are the parts of TYP all.

Fig. 4 provides another embodiment of the invention.Different with blood component segregation apparatus shown in Figure 3, residue blood plasma carrier pipe 6 is connected blood plasma container no longer in advance.Carrier pipe 6 front ends are equipped with hollow needle 31, and outer mask medicated cap 32.There is one to mix mouth on the blood plasma container 2, is covered with the sealing member 30 that can pierce through above.In this embodiment, blood plasma container 2 and blood vessel 19 via can be anastomosing path blockade mechanism 33 interconnect.In Fig. 4 and Fig. 1, all label numerals are identical, all the parts of TYP.

Fig. 5 provides another embodiment of the present invention.Different with blood component segregation apparatus shown in Figure 1, residue blood plasma carrier pipe 6 is connected blood plasma container 2 no longer in advance.Carrier pipe 6 front ends are equipped with hollow needle 34, and outer mask medicated cap 35.In Fig. 5 and Fig. 1, all identical label numerals are all represented the same parts.

Fig. 6 provides another embodiment of the invention.Different with blood component segregation apparatus shown in Figure 1, blood plasma container 2 and freezing precipitation thing solvent container 12 are interconnection no longer in advance.There is one to mix mouth on the solvent container 12, is stamped the sealing member 34 that can puncture.Freezing precipitation thing solvent delivery pipe 11 is independently, and respectively there are hollow needle 36 and 37 in two ends, cover respectively with medicated cap 38 and 39.In Fig. 6 and Fig. 1, all identical label numerals, all parts of TYP.

Fig. 6 A provides another embodiment of the invention.Different with blood component segregation apparatus shown in Figure 1, blood plasma container 2 and freezing precipitation thing solvent container 12 are interconnection no longer in advance.Be connected to releasing tube 14 on the freezing precipitation thing solvent container 12, the latter have one can be anastomosing path blockade mechanism 13, releasing tube 14 front ends are equipped with outstanding taper adapter 15.Freezing precipitation thing solvent delivery pipe 11 front ends are equipped with hollow needle 36, and outer mask medicated cap 38.Among Fig. 6 A and Fig. 1, all label numerals are identical, all the parts of TYP.

Fig. 7 provides another embodiment of the invention.Different with blood component segregation apparatus shown in Figure 1, an end of blood plasma input pipe 1 connects blood plasma container 2, and the other end connects hollow needle 3 in case of necessity.Hollow needle 3 outer masks medicated cap 4.Via have can be anastomosing the residue blood plasma carrier pipe 6 of path blockade mechanism 5, blood plasma container 2 and residue blood plasma container 7 are coupled together.Several mixing mouths can be arranged on the residue blood plasma container 7, be stamped the sealing member 8 and 9 that can puncture.Via have can the anastomosing path blockade 10a of mechanism freezing precipitation thing solvent supply pipe 11, blood plasma container 2 and freezing precipitation thing solvent container 12 are coupled together.Container 12 holds freezing precipitation thing solvent.Also be connected to releasing tube 14 on the container 12, the latter have can be anastomosing path blockade mechanism 13.Releasing tube 14 front ends are equipped with outstanding taper adapter 15, and outer mask medicated cap 16.

In the various embodiments of being quoted as proof, directly connect many conduits on each container in the above.This is requirement not necessarily.Also can be chosen in each container has a pipe, forms many conduits by bearing many branched pipes on this pipe again.

In the various embodiments of being quoted as proof in the above, container and conduit are used soft synthetic resin usually, and the length that preferably softish vinyl chloride resin is made bindiny mechanism's (conduit) of delivery solvent is no more than 50 centimetres, preferably is no more than 25 centimetres.The internal diameter of these connection tubes should be in 1.0 to 3.0 millimeters scopes.When delivery solvent or freezing precipitation thing solution, some solvent or solution can remain in the conduit and can not make full use of, if select the conduit size by above-mentioned requirements, just can reduce this residual part, and course of conveying is steadily carried out.

As for can be anastomosing path blockade mechanism, can utilize any mechanism, as long as guarantee to turn-off at ordinary times stream,, be path of liquid formation in case need just can open.A broken part is preferably arranged, be easy to fracture, to open path.For example, as shown in Figure 8, a hollow pipe 42, its external diameter just in time internal diameter with liquid gateway 41 is the same, the outer end of this hollow pipe 42 is tapered, and at the front end of hollow pipe 42 one and its solid post 43 cast in block is arranged, and the latter's external diameter is less than the internal diameter of liquid gateway 41, but greater than the internal diameter of connecting duct (for example saying conduit 6), hollow pipe 42 and solid post 43 are all used such as the such hard synthetic resin of hard vinyl chloride and are made.Hollow pipe 42 is fixed on the inwall of liquid gateway 41.This energy circuit closing organ has a circle groove 44 in hollow pipe one side near the intersection between hollow pipe 42 and the solid post 43.Before use, the path between solid post 43 blocking-up containers (for example saying container 2) and the conduit (for example saying conduit 6).When preparing to use, apply external force with finger tip, cut off solid post 43 at groove 44 places, the inside of hollow pipe 42 just becomes the passage that circulates between container and conduit.If necessary, on the end face of solid post 43 projection 45 can be arranged, it is shaped as the flat board that width equals solid post 43 external diameters, after solid post 43 breaks away from hollow pipe 42, can not block conduit mouth like this.

Fig. 9 has provided another kind and has been applicable to path blockade of the present invention mechanism.Under this situation, path blockade mechanism is contained in 41 the insides, liquid gateway in the container (for example saying container 2), on the inboard of container.It comprises a pipe 47, and its external diameter just in time internal diameter with liquid gateway 41 is the same, also comprises a post 46, and at the front end and pipe 47 cast solids of pipe 47, the both uses such as the such hard synthetic resin manufacturing of hard vinyl chloride.Post 46 and manage 47 have one the circle groove 48.Before the use, post 46 blocking-up liquid communication.When preparing to use, pull curved post 46, apply external force at groove 48 places with finger and cut off post 46, container (for example saying container 2) and pipe (for example saying conduit 6) are connected.

In Figure 10, there is a partition wall 50 in 49 inner length half place in liquid gateway, is used for block via.When opening path, can peel off the cover 52 on the rubber plug 51,, continue the saturating partition wall 50 of thorn again with a saturating rubber plug 51 of hollow acupuncture.If there is not rubber plug 51,, can cause inevitably that liquid flows backwards when when 50 li of partition walls are extracted hollow needle.Also can select to seal the liquid gateway, replace rubber plug 51 and wrapping its cover 52 with the sealing member of the densification that can sting.

In the situation of blood component segregation apparatus shown in Figure 1, the blood of gathering from the blood transfusion alms giver enters blood vessel 19 by gathering pin 21 and conduit 18, and the blood that container is 19 li divides protometrocyte part and blood plasma partly through centrifugalize.Via blood plasma input pipe 1 blood plasma is partly introduced blood plasma container 2 then, freezing, thaw, centrifugalize again is divided into freezing precipitation thing layer and lacks the plasma layer of freezing precipitation thing.By lacking freezing precipitation thing blood plasma carrier pipe 6, guide to scarce freezing precipitation thing blood plasma container 7 with floating over top scarce freezing precipitation thing plasma layer.Take off the container 7 that lacks freezing precipitation thing blood plasma is housed, send to and make some other blood plasma preparations.Then,, and on an appropriate length, cut off, open path blockade mechanism 10, set up distribution channel carrier pipe 6 sealings.Freezing precipitation thing solvent is via freezing precipitation thing solvent supply pipe 11, enter blood plasma container 2 for 12 li from freezing precipitation thing solvent container, this solvent is to be made of one or more the mixture in the following substances: such as glycine, lysine, alanine, this class water-soluble amino acid of paddy acyl ammonia; Such as sucrose, glucose, these saccharides of mannitol; Such as sodium chloride, this class neutral salt of potassium chloride.Freezing precipitation thing solution in 2 li acquisitions of container sends back to solvent container 12 again.On a suitable position, seal conduit 11, and cut off it with a clamp.Independently container 12 is heated to set point of temperature (for example, in 40 ℃ to 70 ℃ scopes), carries out centrifugalize then.Form the upper strata that protein content makes moderate progress after the centrifugalize, separate, can discharge via releasing tube 14 as the former freezing precipitation thing of defibrination (being platelet cofactor).

The platelet cofactor that discharges is removed impurity through filtering (for example using a filter paper), sends into a bottle lyophilization, is used to produce some preparation.In addition, the freezing precipitation thing in the aforesaid operations process in the blood plasma container 2 can directly be sent freezing precipitation thing solvent container 12 to by conduit 11, and solvent, and the freezing precipitation thing is dissolved in the solvent.

Under blood component segregation apparatus situation shown in Figure 2, with after Fig. 1 device has been removed scarce freezing precipitation thing blood plasma the samely, will install in advance at the freezing precipitation thing solvent of 24 li of freezing precipitation containers by supply pipe 18a and to send blood plasma container 2 to.Send into an empty always container 26 at the solution of 2 li acquisitions of container subsequently, in this container, heat and carry out centrifugalize.Sealing member 27 exposes and mixes after the mouth, and the former freezing precipitation thing of the defibrination that is obtained can discharge by mixing mouth.

Under the situation of blood component segregation apparatus shown in Figure 3, from the solvent injection blood plasma container 2 of solvent container 12.Blood plasma container 2 is delivered centrifugalize together with its content.Discharge the former freezing precipitation thing of defibrination by release catheter 18b then.

Under the situation of blood component segregation apparatus shown in Figure 4, after in device shown in Figure 3, blood plasma being carried out centrifugalize, the hollow needle 31 of carrier pipe 6 front ends is inserted the mixing mouth of 30 li of the sealing members that can puncture.After this step,, discharge the former freezing precipitation thing of defibrination through releasing tube 18b as device shown in Figure 3.

Under the situation of blood component segregation apparatus shown in Figure 5, resemble the device shown in Figure 1 blood plasma carried out centrifugalize after, the hollow needle 34 of carrier pipe 6 front ends is inserted the mixing mouth of 30 li of the sealing members that can puncture.After this step,, discharge the former freezing precipitation thing of defibrination through releasing tube 14 as device shown in Figure 3.

Under the situation of blood component segregation apparatus shown in Figure 6, lack freezing precipitation thing blood plasma container 7 during beginning and be not connected with blood plasma container 2.Only separating the freezing precipitation thing blood plasma and will remove it time of falling vacant, two containers just link together, and the blood plasma container 2 of also getting along well during solvent container 12 beginnings is connected.Only when donor solvent, both just couple together by carrier pipe 11.In all others, the operation of this device is the same with device shown in Figure 1.

Under the situation of blood constituent segregation apparatus shown in Figure 7, blood plasma by blood plasma input pipe 1, send into blood plasma container 2, freezing, thaw, centrifugalize then, be divided into freezing precipitation thing layer and scarce freezing precipitation thing plasma layer.Float over top scarce freezing precipitation thing blood plasma and send scarce freezing precipitation thing blood plasma container 7 to by lacking freezing precipitation thing blood plasma input pipe 6.Cut off carrier pipe 6 then.Via freezing precipitation thing solvent supply pipe 11, send freezing precipitation thing solvent to blood plasma container 2 from freezing precipitation thing solvent container 12.The solution that is obtained is sent solvent container 12 back to from container 2, is heated to set point of temperature, carries out centrifugalize again.Centrifugalize forms one and contains the low upper strata of protein content, discharges through releasing tube 14 as the former freezing precipitation thing of defibrination (platelet cofactor).

Hereinafter with reference to operational instances, the present invention is described more ad hoc.

Example: 200 milliliters or 400 milliliters of fresh bloods, send blood vessel 19 to through gathering pin 21 and conduit 18, be centrifugated into blood plasma components and hemocyte composition.The blood plasma on upper strata is sent an independently blood bag (not drawing on the figure) to.Then, in a blood component segregation apparatus shown in Figure 1, in independent blood bag, send 400 milliliters of blood plasma to blood plasma container 2 via blood plasma input pipe 1, freezing to-70 ℃, thaw in the constant temperature bath between 2 ℃ to 4 ℃ suddenly then.The container 2 that fills blood plasma is put on the centrifugal separator, separated 20 minutes with the acceleration of 3000G.By carrier pipe 6 the scarce freezing precipitation thing blood plasma on upper strata is delivered to scarce freezing precipitation thing blood plasma container and go for 7 li, at 2 li also remaining 7 milliliters of freezing precipitation things of blood plasma container.Then, by solvent supply pipe 11, inject 15 milliliters of solvents from freezing precipitation thing solvent container 12 for 2 li to blood plasma container, the freezing precipitation thing is dissolved in the glycine solution of sodium chloride-containing, solvent contains 13.3 milligrams of glycine and 4 milligrams of sodium-chloride water solutions for every milliliter.Resulting solution is sent solvent container 12 back to, and heating is 5 minutes under 54 ℃ of temperature, again with the acceleration centrifugalize of 1800G 20 minutes.The formed upper strata of centrifugalize is discharged through releasing tube 14, can obtain 18 milliliters of former freezing precipitation things of defibrination, the concentration that contains platelet cofactor is every milliliter 16 iu.

As mentioned above, the objective of the invention is the device of a separating blood constituents, it is characterized in that: comprise a blood plasma container, an end of it and blood plasma input pipe is connected; Comprise a scarce freezing precipitation thing blood plasma container, it can anastomosing mode connect (maybe can connect) blood plasma container by a conduit that is used for carrying the scarce freezing precipitation thing blood plasma of separating in blood plasma container with a kind of; Comprise the means of communication that has the liquid communication path, it can anastomosing mode connect (maybe can connect) blood plasma container with a kind of; Also comprise a container that holds freezing precipitation thing solvent, this solvent can inject blood plasma container by bindiny mechanism.Thereby, this device of the present invention can be isolated the freezing precipitation thing that contains the high concentration platelet cofactor, and can therefrom remove Fibrinogen in blood plasma, usually content is very high in the freezing precipitation thing for the latter, is a reason that causes secondary reaction when a large amount of conveying platelet cofactor.

If in advance blood plasma container, lack freezing precipitation thing blood plasma container and freezing precipitation thing solvent container interconnection good, this device is done as a wholely can constitute a closed system, can not cause air pollution when the conduit that connects each container is operated.In addition, this device is easy and simple to handle.If freezing precipitation thing solvent container is that separately independently this container just can carry out disinfection with autoclave sterilizer, and the other parts of this device can be with gas or radiation sterilization, without autoclave sterilization.Like this, this device is made simple and easy.If freezing precipitation thing solvent container be placed on blood plasma container below, inject the freezing precipitation thing solvent of going up surface vessel from following surface vessel and directly contact with the freezing precipitation thing, thus the freezing precipitation thing dissolve ratio comparatively fast.In addition, rely on gravity, dissolved freezing precipitation thing is sent to and goes in the solvent container also to carry out than being easier to.

Claims (10)

1, a kind of device that is used for the separating blood composition is characterized in that, this device comprises: a blood plasma container, and an end of it and a blood plasma delivery conduit is connected; One holds the container of taking residue blood plasma behind the freezing precipitation thing away, and it is used for carrying the residue blood plasma container of separating that lacks the freezing precipitation thing by one in above-mentioned blood plasma container; And the container that has bindiny mechanism, container fills the water-soluble aminoacid as the solvent of freezing precipitation thing, by above-mentioned bindiny mechanism, a kind of can anastomosing mode the connection maybe can connect above-mentioned blood plasma container.

2, in the device that claim 1 is addressed, above-mentioned bindiny mechanism is a conduit, and its end has a pin, and the other end connects above-mentioned freezing precipitation thing solvent container.

3, right is wanted in 2 devices of addressing, above-mentioned bindiny mechanism be provided with one can be anastomosing path blockade mechanism.

4, in the device that claim 3 is addressed, above-mentioned path blockade mechanism is provided with broken parts, makes it to fracture and can open distribution channel.

5, in the device that claim 1 is addressed, the residue blood plasma container of above-mentioned blood plasma container and above-mentioned scarce freezing precipitation thing is used to carry the conduit of the residue blood plasma that lacks the freezing precipitation thing interconnection by one.

6, in the device that claim 1 is addressed, the residue blood plasma container of above-mentioned blood plasma container and above-mentioned scarce freezing precipitation thing can be used to carry the conduit of the residue blood plasma that lacks the freezing precipitation thing interconnection by one.

7, in the device that claim 1 is addressed, above-mentioned freezing precipitation thing solvent is a kind of neutral aqueous solution, and it contains a kind of material that belongs in water-soluble aminoacid, saccharide and the neutral salt three major types at least, as the stabilizing agent of platelet cofactor.

8, in the device that claim 7 is addressed, above-mentioned water-soluble aminoacid is meant glycine, lysine, alanine or paddy acyl ammonia.

9, in the device that claim 7 is addressed, above-mentioned saccharide is meant sucrose, glucose or mannitol.

10, in the device that claim 7 is addressed, above-mentioned neutral salt is meant sodium chloride or potassium chloride.

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