CN102268828B - Method for deinking waste paper by using multiple enzymes produced from two kinds of strains - Google Patents
Method for deinking waste paper by using multiple enzymes produced from two kinds of strains Download PDFInfo
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- CN102268828B CN102268828B CN2011101964528A CN201110196452A CN102268828B CN 102268828 B CN102268828 B CN 102268828B CN 2011101964528 A CN2011101964528 A CN 2011101964528A CN 201110196452 A CN201110196452 A CN 201110196452A CN 102268828 B CN102268828 B CN 102268828B
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- deinking
- schizophyllum
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- waste paper
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/64—Paper recycling
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Abstract
The invention discloses a method for deinking waste paper by using multiple enzymes produced from two kinds of strains, which relates to a waste paper biological deinking technology in the paper manufacturing industry. The method comprises the following steps of: (1) blending two kinds of schizophyllum commune for liquid submerged fermentation culture; (2) detecting the activity of cellulase, xylanase and lipase and the amount of Schizophyllan in the blended fermentation liquor; (3) pulping waste paper; (4) adding properly diluted crude enzyme liquid into the waste paper pulp for reaction; and (5) carrying out floatation deinking on the waste paper pulp after reaction. The drainage capacity of the waste paper pulp deinked with the method is improved obviously, the paper strength is increased slightly, the whiteness is improved, and the amount of residual ink is reduced obviously. Compared with the traditional method in which a compound enzyme preparation is used, the method disclosed by the invention can be used for reducin the usage amount of a surfactant for flotation and reducing the cost for deinking and the pollution caused by deinking waste water. Thus, the invention provides a new process method for realizing efficient and clean deinking in the paper manufacturing industry.
Description
Technical field
The present invention relates to the waste paper Biologic Deinking technology in paper industry.
Background technology
Secondary stock is a kind of important fiber source of paper industry, uses it to protect forest resources, and environmental contamination reduction reduces the consumption of water and the energy.And deinking is to limit maximum technological difficulties using more on a large scale secondary stock.Traditional deinking process is used a large amount of chemicalss, is the main source of solid and liquid debris, and COD and BOD loading are large, and the wastewater treatment expense is high; Paper performance descends, easy brightness reversion.Therefore should find a kind of efficiently, deinking process that pollutional load is little.The effective ways that enzymatic deinking addresses these problems just.It can reduce the consumption of chemicals, reduces COD and BOD content in waste water, improves water filtering performance and the physical strength of paper pulp.
At present, the enzyme preparation for deinking research has cellulase, hemicellulase (zytase), lignin degradation enzyme (being mainly laccase), lipase, esterase, amylase and pectase.The Enzymatic Deinking technology has experienced single enzyme process, two stages of combined-enzyme method.Single enzyme process, because Deinking Efficiency is low, is seldom studied now.Due to the selectivity of enzyme, determined enzyme can only act in deinking process certain a bit, in order to promote to greatest extent the carrying out of deinking, should draw the advantage of various enzymes, so complex enzyme becomes the focus of Enzymatic Deinking research.At present the combined-enzyme method Deinking Technology generally takes to add that the method for enzyme preparation carries out.Have in this way some problems in the preparation of enzyme preparation, such as carrying out enzyme purification, the separation and purification of enzyme is very loaded down with trivial details, the comparison of technology complexity, and can be accompanied by the loss that enzyme is lived in the purification process of enzyme, cause larger waste.
Summary of the invention
Not only can produce a large amount of schizophans during the schizophyllum commune fermented and cultured, also produce some important enzymes simultaneously.There are some researches show, schizophyllum commune can produce cellulase, zytase, lipase, esterase, manganese peroxidase etc. under specific condition of culture, if can coexist these several enzymes in the fermentation crude enzyme liquid simultaneously, its deinking efficiency to secondary stock is worth research.The present invention is directed to the shortcoming of existing biological enzyme Deinking Technology, produce the characteristics of enzyme according to schizophyllum commune, providing a kind of utilizes two bacterium to produce the completely new approach that multienzyme carries out deinking, the method is produced multienzyme by two kinds of schizophyllum communes of Mixed culture, the crude enzyme liquid that directly utilizes it to contain Schizophyllum commune Fr polysaccharides and plurality of enzymes is processed secondary stock, do not need various enzymes are purified separately, reduce the consumption of floatation and ink removing surfactant simultaneously, reduction deinking cost hangs down the pollution with deinking wastewater.
In order to achieve the above object, the present invention has taked following technical scheme:
(1) two kind of schizophyllum commune carries out the liquid deep layer fermenting cultivation jointly;
The model of schizophyllum commune can be following any two kinds: Schizophyllum commune ZM37.8, Schizophyllum commune ATCC38548, Schizophyllum commune Fr., Schizophyllum sp.F17, Schizophyllum commune AS5.39.
Fermentation temperature: 10-30 ℃, fermentation pH:3-9, fermentation time: 4-15 days
Fermentation medium forms: the 5-30g/L such as cellulose, wheat bran, rice straw powder or cornstalk, olive oil, peanut oil, rapeseed oil, corn wet goods 1-20mL/L, peptone 5g/L, (NH
4)
2sO
42g/L, Mandel nutrient solution 70mL/L (KH
2pO
44g/L, MgSO
47H
2o0.3g/L, CaCl
20.3g/L, FeSO
47H
2o50mg/L, MnSO
47H
2o1.56mg/L, ZnSO
41.4mg/L, CoCl
20.2mg/L)
(2) detect vigor and the Schizophyllum commune Fr polysaccharides content of mixed culture fermentation liquid cellulase, zytase and lipase;
(3) size degradation waste paper;
(4) in the secondary stock after will joining size degradation through the crude enzyme liquid of suitable dilution, react;
Enzyme liquid consumption: the 0.1-10U/g oven dry stock, calculate according to cellulase activity (carboxymethylcelluloenzyme enzyme, filter paper enzyme or avicelase);
Starch dense: 1-20%; PH value: 3-8; Temperature: 20-50 ℃; Reaction time: 20-90min;
(5) reacted secondary stock carries out floatation and ink removing;
Dosage of surfactant is 0.01%-0.5%;
Wherein (2), (3) and (5) step are the conventional treatment step, for well known to those of ordinary skill in the art, can be undertaken by the general practice.
Biology enzyme can act on the interface between printing ink and fiber selectively, impels the swollen of fiber, and being connected between fiber and printing ink weakened.Recorded cellulase, zytase and lipase in two kinds of schizophyllum commune crude enzyme liquids cultivating in the present invention, its cellulase and zytase can change near linking bond fiber surface or ink particle, and lipase is the degrading plant oil-based ink.
The invention has the advantages that: the first, realized the Mixed culture of two kinds of schizophyllum communes; The second, produced cellulase, zytase and lipase etc. during this two kinds of schizophyllum commune Mixed culture, these enzyme Cooperative Deinkings, can realize deinking efficiency preferably; The 3rd, directly utilize the crude enzyme liquid that contains multienzyme for deinking, do not need various enzymes are carried out to purification processes; The 4th, also have a large amount of Schizophyllum commune Fr polysaccharides in the schizophyllum commune crude enzyme liquid, booster action is arranged in floatation and ink removing, can reduce the consumption of surfactant, reduce the pollution of deinking cost and deinking wastewater.
The specific embodiment
Below in conjunction with concrete experimental data, deinking efficiency of the present invention is described
The two bacterium multi-enzyme methods of example are processed waste newsprint
Two kinds of schizophyllum communes (Schizophyllum commune ZM37.8 and Schizophyllum commune ATCC38548) carry out liquid deep layer fermenting, and nutrient media components is cellulose 20g/L, olive oil 2mL/L, peptone 5g/L, (NH
4)
2sO
42g/L, Mandel nutrient solution 70mL/L (KH
2pO
44g/L, MgSO
47H
2o0.3g/L, CaCl
20.3g/L, FeSO
47H
2o50mg/L, MnSO
47H
2o1.56mg/L, ZnSO
41.4mg/L, CoCl
20.2mg/L), 27 ℃ of temperature, pH5.5; Cultivate and within 7 days, measure that enzyme work is respectively cellulase (carboxymethylcelluloenzyme enzyme is lived) 4.20U/mL, xylanase activity is that 245.39U/mL, lipase activity are 1.27U/mL, Schizophyllum commune Fr polysaccharides content is 0.7g/L.Crude enzyme liquid is added to reaction in the old and useless newspaper slurry through discongesting processing, the crude enzyme liquid addition is pressed cellulase activity and is calculated the 0.5U/g oven dry stock, starch and densely be adjusted to 10%, treatment temperature is 50 ℃, the pH value is 5.5, processing time is 40min, in processing procedure, every 10min, paper pulp is rubbed, to guarantee the uniformity of reaction.Paper pulp, after enzyme is processed, is starched and densely is adjusted to 1%, adds neopelex 0.05%, carries out floatation and ink removing, and temperature is 25 ℃, and till flotation to non-foam is overflowed, handsheet, measure Paper White Degree, residual ink amount and paper property indices.Result shows, with blank sample (not enzyme-added liquid only adds surfactant and carries out flotation), compare, crude enzyme liquid deinking sample whiteness improves 3%ISO, and effectively the residual ink amount descends 51.3%, and do not reduce paper tensile index and burst index, and tear index even slightly is improved.
Claims (3)
1. one kind is utilized two bacterium to produce the method that multienzyme carries out deinking, it is characterized in that comprising the following steps:
(1) two kind of schizophyllum commune carries out the liquid deep layer fermenting cultivation jointly, and the model of schizophyllum commune is following any two kinds:
Schizophyllum?commune?ZM37.8、Schizophyllum?commune?ATCC38548、Schizophyllum?commune?Fr.、Schizophyllum?sp.F17、Schizophyllum?commune?AS5.39；
(2) under the Mixed culture condition, two kinds of common cellulase-producings of schizophyllum commune, zytase and lipase; Fermentation temperature: 10-30 ℃, fermentation pH:3-9, fermentation time: 4-15 days, fermentation medium forms: cellulose, wheat bran, rice straw powder or cornstalk 5-30g/L, olive oil, peanut oil, rapeseed oil, corn oil 1-20mL/L, peptone 5g/L, (NH
4)
2sO
42g/L, Mandel nutrient solution 70mL/L, wherein the Mandel nutrient solution is by KH
2pO
44g/L, MgSO
47H
2o0.3g/L, CaCl
20.3g/L, FeSO
47H
2o50mg/L, MnSO
47H
2o1.56mg/L, ZnSO
41.4mg/L, CoCl
20.2mg/L preparation;
(3) directly use the crude enzyme liquid of two kinds of schizophyllum commune fermentations to carry out the deinking processing to secondary stock.
2. method according to claim 1, in the deinking reaction, the crude enzyme liquid consumption is the 0.1-10U/g oven dry stock, press the vigor calculating of cellulase, described cellulase is carboxymethylcelluloenzyme enzyme, filter paper enzyme or avicelase.
3. method according to claim 1, the dosage of surfactant of using in deinking is 0.01-0.5%.
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| CN2011101964528A CN102268828B (en) | 2011-07-13 | 2011-07-13 | Method for deinking waste paper by using multiple enzymes produced from two kinds of strains |
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| CN102268828B true CN102268828B (en) | 2013-12-11 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103335977B (en) * | 2013-06-27 | 2015-09-09 | 中国制浆造纸研究院 | The assay method of ink content in a kind of deinking wastewater |
| CN105862498B (en) * | 2016-03-29 | 2017-11-24 | 山东贵和显星纸业有限公司 | The preparation method and applications of paper grade (stock) recycled fiber modified enzyme |
| CN107119482B (en) * | 2017-06-16 | 2019-01-01 | 南京陶特思软件科技有限公司 | A kind of room temperature removal ink pulping process |
| CN107119480B (en) * | 2017-06-16 | 2018-08-24 | 江苏浩宇电子科技有限公司 | A kind of waste paper recycles low ink pulping process |
| CN107245894B (en) * | 2017-06-16 | 2019-04-05 | 南京陶特思软件科技有限公司 | A kind of waste paper recycles the preparation method of paper sludge |
| CN108425268B (en) * | 2018-03-03 | 2020-09-18 | 浙江高度环保科技有限公司 | Coated paper and preparation method thereof |
| CN110528304A (en) * | 2019-08-27 | 2019-12-03 | 绵阳禾本生物工程有限公司 | A kind of high-efficiency environment friendly Biologic Deinking Agent |
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| US6241849B1 (en) * | 1998-09-17 | 2001-06-05 | Novo Nordisk Biochem North America, Inc. | Methods for deinking and decolorizing printed paper |
| CN1664026A (en) * | 2004-03-02 | 2005-09-07 | 比奥生物科技（深圳）有限公司 | Component and using method of bioenzyme deinking agent for mixed news waste papers |
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| US6576083B2 (en) * | 2001-04-05 | 2003-06-10 | Novozymes A/S | Deinking of waste paper |
| JP2003201690A (en) * | 2001-12-27 | 2003-07-18 | Mitsubishi Paper Mills Ltd | Enzymatically treated waste newspaper pulp, base paper using the pulp, and coated paper using the base paper |
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Patent Citations (2)
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| US6241849B1 (en) * | 1998-09-17 | 2001-06-05 | Novo Nordisk Biochem North America, Inc. | Methods for deinking and decolorizing printed paper |
| CN1664026A (en) * | 2004-03-02 | 2005-09-07 | 比奥生物科技（深圳）有限公司 | Component and using method of bioenzyme deinking agent for mixed news waste papers |
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| JP特开2003-201690A 2003.07.18 |
| 张燕兴等.裂褶菌发酵培养产酶条件的优化及用于新闻纸脱墨的研究.《造纸科学与技术》.2011,第30卷(第3期),第71-74. |
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Address after: 510300 No. 154 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee after: Guangdong Paper Research Institute Co.,Ltd. Address before: 510300 No. 154 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee before: GUANGDONG PAPER INDUSTRIAL Research Institute |
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Granted publication date: 20131211 Termination date: 20210713 |