CN102260181A - Method for extracting amino acids from sugarcane toppers - Google Patents
Method for extracting amino acids from sugarcane toppers Download PDFInfo
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- CN102260181A CN102260181A CN201110124263XA CN201110124263A CN102260181A CN 102260181 A CN102260181 A CN 102260181A CN 201110124263X A CN201110124263X A CN 201110124263XA CN 201110124263 A CN201110124263 A CN 201110124263A CN 102260181 A CN102260181 A CN 102260181A
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Abstract
The invention discloses a method for extracting amino acids from sugarcane toppers. The method comprises the following steps of: pressing squeezed and cut sugarcane toppers to obtain juice; filtering the juice to remove clays and sugarcane slag in the juice in a vacuum state; dynamically adsorbing tyrosine and phenylalanine by using an active carbon after adsorbing and discoloring the sugarcane topper juice by using an active carbon; dynamically eluting the sugarcane topper juice by using ammonia water and ethanol; collecting an eluent; concentrating and crystallizing the eluent to obtain crude tyrosine and crude phenylalanine; and separating out histidine, lysine and arginine by using an ion exchange adsorption method. According to the invention, by using the method of adsorbing by using the active carbon adsorption and then separating by using the ion exchange resin, the utilization rate of the raw materials is increased, the requirements on the equipment are reduced, the working efficiency is enhanced, and the overlapping phenomenon among the phenylalanine, the tyrosine and the histidine during pure ion exchange is also preferably solved.
Description
Technical field
The invention belongs to a kind of amino acid whose method of from sugarcane toppers, extracting.
Background technology
The sugarcane tip is sugarcane stem vegetative point (sugarcane Huang) position, place, and in the sugarcane production growth course, the nutriment of sugarcane stem offers vegetative point as far as possible, so it contains rich nutrient substances, as protein, starch, amino acid, VITAMIN, enzyme and mineral substance etc.Amino acid whose content is in the sugarcane stem about about 5 times in the sugarcane tip.Amino acid is the fundamental unit that constitutes the organism protein molecule, with the vital movement of biology confidential relation is arranged.It has special physiological function in body, be one of indispensable nutritive ingredient in the organism.
Amino acid is except that having well-known trophism, and medical research department has confirmed some amino acid whose medical value in recent years, as arginine, L-glutamic acid as one of hepatic coma salvage drug; Histidine is used for the treatment of gastric and duodenal ulcer and hepatitis; Methionine(Met) is used for the treatment of liver cirrhosis and fatty liver; Methionin, methionine(Met) promote childhood development in a large number as nutrient fortified food; Asparagine is treatment heart trouble and hypertensive good medicine etc.
Guangxi sugarcane annual production surpasses 4,400 ten thousand tons, and the amount of the sugarcane sugarcane tip is very big, but the sugarcane tip is almost useless to sugar industry, cut the millions of tons of sugarcane tips that cut off every year or gone back the field, or do animal feed, and little to the degree of its processing and utilization, do not bring into play resources advantage.If can do the research of some deep processing aspects to it according to the composition of the sugarcane sugarcane tip, fully develop its potential value, benefit to driving local economy.
Summary of the invention
The technical problem that the present invention requires to solve provides a kind ofly can turn waste into wealth, abundant sharp resource, do raw material with sugarcane toppers and extract amino acid whose method.
The present invention solves the problems of the technologies described above with following technical scheme:
(1) the sugarcane toppers juice that sugarcane toppers is squeezed out, at first remove earth, bagasse in the juice with vacuum filtration, again with the decolouring of gac Static Adsorption, during decolouring Normal juice is diluted 50 times, pH is 2～5, activated carbon dosage 2～6g/100mL, adsorption time are 50～150min, and Heating temperature is 50 ℃～90 ℃;
(2) tyrosine and the phenylalanine in the use gac dynamic adsorption sugarcane tip juice, the consumption of gac is 1～5g/100mL, pH is 5～6; Use ammoniacal liquor and ethanol dynamic desorption tyrosine and phenylalanine from gac again, the concentration of ammoniacal liquor is 0.5～2.0mol/L, and concentration of ethanol is 30%～90%;
(3) separate basic aminoacids (Histidine, Methionin, arginine) with ion exchange adsorption, eluent is one or both in following five kinds: NH
4Cl, NH
4Cl-NH
3H
2O (1: 1), NH
3H
2O, NH
4COOH-NH
3H
2O (1: 1), NH
4COOH, flow velocity are 1～3mL/min.
The pH of the described gac Static Adsorption decolouring of step 1 is 4, and Heating temperature is 80 ℃, and adsorption time is 75min, and activated carbon dosage is 4g/100mL.
When step 2 described gac dynamic adsorption tyrosine and phenylalanine, flow rate control is at 1～1.5mL/min.
Step 2 is described from gac during dynamic desorption amino acid, and earlier with distillation washing post, the control flow velocity is at 2～3mL/min, till not having a ninhydrin reaction; Use the ammoniacal liquor wash-out then, the control flow velocity is about 2～3mL/min, and substep is collected, and cooperates the Pauly reaction to check, merges tyrosine and shows the light red part, obtains the tyrosine elutriant; Use 60% ethanol elution again instead, till not having a ninhydrin reaction, obtain the phenylalanine elutriant.
Gac of the present invention will pass through pre-treatment, with NaOH solution, 0.5mol/L HCL solution, tap water and the distilled water processed in sequence of 0.5mol/L, cleans immersion, when treating that its pH value becomes neutrality it is dried.
During the described separation basic aminoacids of step 3, best upper prop and the elution flow rate of utilizing ion exchange method to extract three kinds of basic aminoacidss from sugarcane tip juice solution are 1mL/min; If need three kinds of basic aminoacidss of preparation, can be respectively with the NH of three kinds of different concns
3H
2O (0.05mol/L, 0.1mol/L, 2mol/L) wash-out: earlier with 0.05mol/L ammoniacal liquor wash-out (eluent flow rate is about 1mL/min), wait to flow out about 20mL solid volume after; The beginning substep is collected, every pipe 10mL, with 0.1mol/L ammoniacal liquor wash-out, be eluted to no ninhydrin reaction, use 2mol/L ammoniacal liquor wash-out again, be washed till ninhydrin reaction and detect till the essentially no colour developing, carry out chromatography to collecting elutriant, behind the chromatography 2h, utilize pauly reagent to detect and the definite liquid composition of collecting of slope oral examination agent detection, carry out crystallization and recrystallization again and obtain purified Histidine, Methionin, arginine.If the single arginine of preparation adopts NH
4COOH and NH
4COOH-NH
3H
2O is used in combination, and at first uses the NH of 0.2mol/L
4COOH-NH
3H
2O wash-out Histidine, Methionin when being washed till no ninhydrin reaction, are washed till no COOH with deionized water
-, use 0.1mol/LNH again
3H
2O and 1mol/L NH
3H
2O is wash-out in succession, gets single arginine, so not only can make Histidine, Methionin obtain better to separate with arginine, and the while saves eluent again and product does not contain NH
4COOH, easily purifying.
Ion exchange resin of the present invention will pass through pre-treatment, and 732 resin cation (R.C.)s are put into beaker, adds excessive distilled water immersion 24h, stirs resin, removes the broken resin of suspension.Then, the about 3h of HCl immersion with 2mol/L is washed till pH5～6 with distilled water, uses the NaOH of 2mol/L to soak 3h then, is washed till pH5～6 with distilled water again, and the HCl with 2mol/L does the processing that makes the transition at last, is washed till neutrality with distilled water.
Method remarkable advantage of the present invention has: applied activated carbon decolours and adsorbs phenylalanine and tyrosine, improved utilization ratio of raw materials greatly, reduced requirement to equipment, improved working efficiency, and the charcoal absorption filtering impurity in the upper prop liquid, also solved the problem that resin easily poisons in the simple separation exchange column; Separate the reasonable juxtaposition phenomenon that has solved between simple ion-exchange phenylalanine, tyrosine and the Histidine with spent ion exchange resin after the charcoal absorption earlier simultaneously.
Embodiment
The invention provides the means that a kind of physics and chemical process combine and from sugarcane toppers, extract amino acid whose method.In the method for the invention, with Activated Carbon Adsorption Separation tyrosine and phenylalanine, separate basic aminoacids with ion exchange adsorption, the selection of eluent and separation condition is to realize key of the present invention.Present method is being carried out behind the vacuum filtration sugarcane toppers juice, and gac is 2～5 at pH, activated carbon dosage 2～6g/100mL, adsorption time are that 50～150min, Heating temperature are the pre-treatment of under 50 ℃～90 ℃ the condition sugarcane toppers juice being decoloured; Pretreated sugarcane toppers juice is that 1～1.5mL/min, pH adsorb under 5.8 the condition through gac at flow velocity, use 1.5mol/L ammoniacal liquor wash-out then, the control flow velocity is about 2～3mL/min, substep is collected, and cooperates the inspection of Pauly color reaction, to the tyrosine colour developing of turning out cloudy, merge and contain the tyrosine part, use 60% ethanol elution again instead, till not having a ninhydrin reaction, elutriant through the decolouring low temperature crystallization filter the crude product of phenylalanine and tyrosine; Filtrate is spent ion exchange resin absorption exchange again, and elution flow rate is 1mL/min; Eluent is one or both in following five kinds: NH
4Cl, NH
4Cl-NH
3H
2O (1: 1), NH
3H
2O, NH
4COOH-NH
3H
2O (1: 1), NH
4COOH, the crystallization of elutriant concentrating under reduced pressure gets Histidine, Methionin, arginine crude product, again through the decolorization filtering crystallization dry refining propylhomoserin.
Embodiment 1
Get sugarcane toppers and squeeze into juice, filter, macromole impurity such as elimination earth with 50 times of filtered juice dilutions, are used activated carbon decolorizing, decolouring pH value is 4.0, Heating temperature is 80 ℃, and activated carbon dosage is 4g/100mL, and bleaching time is 75min, gac reaches 75.37% to the percent of decolourization of sugarcane tip juice solution, and the amino acid rate of loss is 13.75%; Getting filtered juice accent pH after the filtration is 5.8, is that 1～1.5mL/min flows into adsorption column internal adsorption tyrosine and the phenylalanine that contains the 100g gac with flow velocity, cooperates the ninhydrin reaction inspection, to the red-purple reaction is arranged, promptly stops upper prop; Earlier with distillation washing post, the control flow velocity is about 2～3mL/min, till not having a ninhydrin reaction; Use 1.5mol/L ammoniacal liquor wash-out then, the control flow velocity is about 2～3mL/min, and substep is collected, and cooperates the Pauly reaction to check, till the Panly reaction does not show light red, merges and contains the tyrosine coloured moiety of turning out cloudy, and obtains the tyrosine elutriant; Use 60% ethanol elution again instead, till not having a ninhydrin reaction, obtain the tyrosine elutriant.The tyrosine elutriant is caught up with ammonia with the rotatory evaporator concentrating under reduced pressure, and adjust pH is 2, adds the decolouring of 2% activated carbon granule, filter, filtrate is cooled to room temperature, places careful separation filtrate two days down for 4 ℃ in the refrigerator, the adularescent needle-like crystal is separated out, filter tyrosine and filtrate, recrystallization is once used 95% washing with alcohol again, place 60 ℃ in baking oven dry the tyrosine finished product, ply of paper is analysed to detect and is single spot.Tyrosine filtered liquid and phenylalanine elutriant are mixed, concentrate purification step with tyrosine, get the phenylalanine crystal, ply of paper is analysed to detect and is single spot.732 resin cation (R.C.)s that 50mL is disposed place in the ion exchange column of 1 * 40cm, get 200mL charcoal absorption phenylalanine, sugarcane tip juice amino acid solution upper prop behind the tyrosine, flow rate control is at 1mL/min, when Histidine color reaction (being that the Pauly color reaction detects) is arranged to effluent liquid, till being washed till no amino acid and coming out with deionized water (the absorption triketohydrindene hydrate check under the condition of different temperatures), use 0.05mol/L ammoniacal liquor with about 1mL/min flow velocity wash-out earlier, after waiting to flow through about 20mL solid volume, the beginning substep is collected, every pipe 10mL, then use 0.1mol/L ammoniacal liquor wash-out, be eluted to no ninhydrin reaction, use 2mol/L ammoniacal liquor wash-out again, be washed till ninhydrin reaction and detect till the essentially no colour developing, collect elutriant 86 pipes altogether.The elutriant that the 15-32 pipe is contained Histidine carries out concentrating under reduced pressure, has waited to crystallize out, and transfers to pH2.5 with hydrochloric acid while hot, and adds 95% dehydrated alcohol that is twice in solution amount, staticly settles, and filters, and gets the Histidine crude product; Crude product is dissolved in the deionized water, and heating for dissolving transfers to pH2.5 with hydrochloric acid, the gac that adds 1.5% solution amount decolours, filters, and filtrate adds 95% dehydrated alcohol of two times of solution amount, crystallisation by cooling, filter the back oven drying at low temperature, ply of paper is analysed to detect and is L-Histidine finished product.The elutriant that the 37-55 pipe is contained Methionin is evaporated to syrupy shape, transfers to about pH4 with hydrochloric acid, adds 95% dehydrated alcohol of three times of solution amount, and cooling is placed, and filters, and gets the Methionin crude product; In deionized water, heating for dissolving adds 1.5% activated carbon decolorizing with dissolving crude product, filters, and is evaporated to crystallization again and occurs, and adds 95% dehydrated alcohol of two times of solution amount, and crystallisation by cooling filters the back oven drying at low temperature, and ply of paper is analysed to detect and is L-Methionin finished product.The 57-86 pipe is contained arginic elutriant carry out concentrating under reduced pressure, waited to crystallize out, transfer to about pH4.0 with hydrochloric acid while hot, and add 95% dehydrated alcohol that is twice in solution amount, staticly settle, filter, get the arginine crude product; The arginine crude product is dissolved in deionized water, and heating for dissolving transfers to pH4.0 with hydrochloric acid, the activated carbon decolorizing that adds 1% solution amount filters, and filtrate adds 95% dehydrated alcohol of two times of solution amount, crystallisation by cooling filters the back oven drying at low temperature, and ply of paper is analysed to detect and is L-arginine finished product.
Embodiment 2
Get sugarcane toppers and squeeze into juice, filter, macromole impurity such as elimination earth with 50 times of filtered juice dilutions, are used activated carbon decolorizing, and decolouring pH value is 3.22, and Heating temperature is 77.8 ℃, and activated carbon dosage is 5g/100mL, and bleaching time is 70min, filters; Get filtered juice 100mL, transferring pH is 6, is that to flow into weight be the adsorption column internal adsorption of 500g gac to 1～1.5mL/min with flow velocity: earlier with distillation washing post, the control flow velocity is about 2～3mL/min, till not having a ninhydrin reaction; Use 2.0mol/L ammoniacal liquor wash-out then, the control flow velocity is about 2～3mL/min, and substep is collected, and cooperates the Pauly reaction to check, till the Panly reaction does not show light red, merges and contains the Tyr coloured moiety of turning out cloudy, and obtains the tyrosine elutriant; Use 50% ethanol elution again instead, till not having a ninhydrin reaction, obtain the phenylalanine elutriant.The tyrosine elutriant is caught up with ammonia with the rotatory evaporator concentrating under reduced pressure, and adjust pH is 2, adds the decolouring of 2% activated carbon granule, filter, filtrate is cooled to room temperature, places careful separation filtrate two days down for 4 ℃ in the refrigerator, the adularescent needle-like crystal is separated out, filter tyrosine and filtrate, recrystallization is once used 95% washing with alcohol again, place 60 ℃ in baking oven dry the tyrosine finished product, ply of paper is analysed to detect and is single spot.Tyrosine filtered liquid and phenylalanine elutriant are mixed, concentrate purification step with tyrosine, get the phenylalanine crystal, ply of paper is analysed to detect and is single spot.732 resin cation (R.C.)s that 50mL is disposed place in the ion exchange column of 1 * 40cm, get the sugarcane tip juice amino acid solution upper prop of 200mL behind charcoal absorption phenylalanine, tyrosine, flow rate control is at 1mL/min, when Histidine color reaction (Pauly reaction detection) is arranged to effluent liquid, till being washed till no amino acid and coming out with deionized water (the absorption triketohydrindene hydrate check under the condition of different temperatures), use the NH4COOH-NH3H2O wash-out of 0.1mol/L then, to there not being ninhydrin reaction, the NH4COOH-NH3H2O with 0.5mol/L continues wash-out again.When the eluent consumption is 150～300mL, wash-out come out for Histidine, the elutriant of Histidine is carried out concentrating under reduced pressure, waited to crystallize out, transferred to pH 2.5 with hydrochloric acid while hot, and added 95% dehydrated alcohol that is twice in solution amount, staticly settle, filter, get the Histidine crude product; In deionized water, heating for dissolving transfers to pH2.5 with hydrochloric acid with the Histidine dissolving crude product, the gac that adds 1.5% solution amount decolours, filters, and filtrate adds 95% dehydrated alcohol of two times of solution amount, crystallisation by cooling, filter the back oven drying at low temperature, ply of paper is analysed to detect and is L-Histidine finished product.When the eluent consumption is 340～690mL, wash-out come out for Methionin, the elutriant of Methionin is evaporated to syrupy shape, transfer to about pH4 with hydrochloric acid, add 95% dehydrated alcohol of three times of solution amount, cooling is placed, filter, the Methionin crude product; The Methionin crude product is dissolved in deionized water, and heating for dissolving adds 1.5% activated carbon decolorizing, filters, and is evaporated to crystallization again and occurs, and adds 95% dehydrated alcohol of two times of solution amount, and crystallisation by cooling filters the back oven drying at low temperature, and ply of paper is analysed to detect and is L-Methionin finished product.When the eluent consumption was 690～920mL, what wash-out came out was arginine, and arginic elutriant is carried out concentrating under reduced pressure, waited to crystallize out, transferred to about pH4.0 with hydrochloric acid while hot, and added 95% dehydrated alcohol that is twice in solution amount, staticly settle, filter, get the arginine crude product; Crude product is dissolved in deionized water, and heating for dissolving transfers to pH4.0 with hydrochloric acid, adds the activated carbon decolorizing of 1% solution amount, filters, and filtrate adds 95% dehydrated alcohol of two times of solution amount, and crystallisation by cooling filters the back oven drying at low temperature, and ply of paper is analysed to detect and is L-arginine finished product.
Embodiment 3
Get sugarcane toppers and squeeze into juice, filter macromole impurity such as elimination earth, with 50 times of filtered juice dilutions, use activated carbon decolorizing, decolouring pH value is 3, Heating temperature is 75 ℃, and activated carbon dosage is 4g/100mL, and bleaching time is 80min, filter, get filtered juice 100mL, transferring pH is 5.5, with flow velocity is that 1～1.5mL/min inflow weight is the adsorption column internal adsorption of 500g gac, earlier with distillation washing post, the control flow velocity is about 2～3mL/min, till not having a ninhydrin reaction; Use 1.0mol/L ammoniacal liquor wash-out then, the control flow velocity is about 2～3mL/min, and substep is collected, and cooperates the Pauly reaction to check, till the Panly reaction does not show light red, merges and contains the tyrosine coloured moiety of turning out cloudy, and obtains the tyrosine elutriant; Use 70% ethanol elution again instead, till not having a ninhydrin reaction, obtain the phenylalanine elutriant.The tyrosine elutriant is caught up with ammonia with the rotatory evaporator concentrating under reduced pressure, and adjust pH is 2, adds the decolouring of 2% activated carbon granule, filter, filtrate is cooled to room temperature, places careful separation filtrate two days down for 4 ℃ in the refrigerator, the adularescent needle-like crystal is separated out, filter tyrosine and filtrate, recrystallization is once used 95% washing with alcohol again, place 60 ℃ in baking oven dry the tyrosine finished product, ply of paper is analysed to detect and is single spot.Tyrosine filtered liquid and phenylalanine elutriant are mixed, concentrate purification step with tyrosine, get the phenylalanine crystal, ply of paper is analysed to detect and is single spot.732 resin cation (R.C.)s that 50mL is disposed place in the ion exchange column of 1 * 40cm, get the sugarcane tip juice amino acid solution upper prop of 200mL behind charcoal absorption phenylalanine, tyrosine, flow rate control is at 1mL/min, when Histidine color reaction (detection of Pauly color reaction) is arranged to effluent liquid, till being washed till no amino acid and coming out with deionized water (the absorption triketohydrindene hydrate check under the condition of different temperatures), NH4COOH with 0.1mol/L is eluted to no ninhydrin reaction then, and the NH4COOH with 0.5mol/L continues wash-out again.When the eluent consumption is 180～320mL, wash-out come out for Histidine, the elutriant of Histidine is carried out concentrating under reduced pressure, waited to crystallize out, transferred to pH2.5 with hydrochloric acid while hot, and added 95% dehydrated alcohol that is twice in solution amount, staticly settle, filter, get the Histidine crude product; In deionized water, heating for dissolving transfers to pH2.5 with hydrochloric acid with the Histidine dissolving crude product, the gac that adds 1.5% solution amount decolours, filters, and filtrate adds 95% dehydrated alcohol of two times of solution amount, crystallisation by cooling, filter the back oven drying at low temperature, ply of paper is analysed to detect and is L-Histidine finished product.When the eluent consumption is 340～750mL, wash-out come out for Methionin, the elutriant of Methionin is evaporated to syrupy shape, transfer to about pH4 with hydrochloric acid, add 95% dehydrated alcohol of three times of solution amount, cooling is placed, filter, the Methionin crude product; Crude product is dissolved in deionized water, and heating for dissolving adds 1.5% activated carbon decolorizing, filters, and is evaporated to crystallization again and occurs, and adds 95% dehydrated alcohol of two times of solution amount, and crystallisation by cooling filters the back oven drying at low temperature, and ply of paper is analysed to detect and is L-Methionin finished product.When the eluent consumption was 750～980mL, what wash-out came out was arginine, and arginic elutriant is carried out concentrating under reduced pressure, waited to crystallize out, transferred to about pH4.0 with hydrochloric acid while hot, and added 95% dehydrated alcohol that is twice in solution amount, staticly settle, filter, get the arginine crude product; Crude product is dissolved in deionized water, and heating for dissolving transfers to pH4.0 with hydrochloric acid, adds the activated carbon decolorizing of 1% solution amount, filters, and filtrate adds 95% dehydrated alcohol of two times of solution amount, and crystallisation by cooling filters the back oven drying at low temperature, and ply of paper is analysed to detect and is L-arginine finished product.
Claims (7)
1. one kind is extracted amino acid whose method from sugarcane toppers, it is characterized in that processing step is:
The sugarcane toppers juice that step 1 pair sugarcane toppers squeezes out, at first remove earth, bagasse in the juice with vacuum filtration, again with the decolouring of gac Static Adsorption, with 50 times of Normal juice dilutions, pH is 2～5, activated carbon dosage 2～6g/100mL, adsorption time are that 50～150min, Heating temperature are 50 ℃～90 ℃ during decolouring;
Step 2 is used tyrosine and the phenylalanine in the gac dynamic adsorption sugarcane tip juice, and pH is 5～6; Use ammoniacal liquor and ethanol dynamic desorption tyrosine and phenylalanine from gac again, the concentration of ammoniacal liquor is 0.5～2.0mol/L, and concentration of ethanol is 30%～90%;
Step 3 is separated basic aminoacids Histidine, Methionin, arginine with ion exchange adsorption, and eluent is one or both in following five kinds: NH
4Cl, ratio are 1: 1 NH
4Cl-NH
3H
2O, NH
3H
2O, ratio are 1: 1 NH
4COOH-NH
3H
2O and NH
4COOH, flow velocity are 1～3mL/min.
2. as claimed in claim 1ly extract amino acid whose method from sugarcane toppers, it is characterized in that the pH of the described gac Static Adsorption decolouring of step 1 is 4, Heating temperature is 80 ℃, and adsorption time is 75min, and activated carbon dosage is 4g/100mL.
3. as claimed in claim 1ly extract amino acid whose method from sugarcane toppers, when it is characterized in that step 2 described gac dynamic adsorption tyrosine and phenylalanine, flow rate control is at 1～1.5mL/min.
4. as claimed in claim 1ly extract amino acid whose method from sugarcane toppers, it is characterized in that step 2 is described from gac during dynamic desorption amino acid, earlier with distillation washing post, the control flow velocity is at 2～3mL/min, till not having a ninhydrin reaction; Use the ammoniacal liquor wash-out then, the control flow velocity is at 2～3mL/min, and substep is collected, and cooperates the inspection of Pauly color reaction, merges tyrosine and shows the light red part, obtains the tyrosine elutriant; Use 60% ethanol elution again instead, till not having a ninhydrin reaction, obtain the phenylalanine elutriant.
5. describedly from sugarcane toppers, extract amino acid whose method as claim 1 or 2 or 3 or 4, it is characterized in that gac of the present invention will pass through pre-treatment, NaOH solution, 0.5mol/L HCL solution, tap water and distilled water processed in sequence with 0.5mol/L, clean to soak, when treating that its pH value becomes neutrality it is dried.
6. as claimed in claim 1ly extract amino acid whose method from sugarcane toppers, when it is characterized in that the described discrete group propylhomoserin of step 3, Methionin, arginine, best upper prop and the elution flow rate of utilizing ion exchange method to extract from sugarcane tip juice solution are 1mL/min; If when needing the above-mentioned three kinds of basic aminoacidss of preparation, can use the NH of 0.05mol/L, 0.1mol/L, 2mol/L respectively
3H
2The O wash-out: be the 0.8-1.2mL/min wash-out with the flow velocity earlier with 0.05mol/L ammoniacal liquor, wait to flow out about 20mL solid volume after, the collection of beginning substep, every pipe 10mL collects elutriant 86 altogether and manages.Use 0.1mol/L ammoniacal liquor wash-out again, be eluted to no ninhydrin reaction, use 2mol/L ammoniacal liquor wash-out at last, being washed till ninhydrin reaction detects till the essentially no colour developing, carry out chromatography to collecting elutriant, behind the chromatography 2h, utilize the pauly colouring reagents to detect and the definite liquid composition of collecting of slope oral examination agent detection, carry out crystallization and recrystallization again and obtain purified Histidine, Methionin, arginine; If the single arginine of preparation adopts NH
4COOH and NH
4COOH-NH
3H
2O is used in combination, and at first uses the NH of 0.2mol/L
4COOH-NH
3H
2O wash-out Histidine, Methionin when being washed till no ninhydrin reaction, are washed till no COOH with deionized water
-, use 0.1mol/LNH again
3H
2O and 1mol/LNH
3H
2O is wash-out in succession, gets single arginine.
7. as claimed in claim 1ly from sugarcane toppers, extract amino acid whose method, it is characterized in that ion exchange resin of the present invention will pass through pre-treatment, 732 resin cation (R.C.)s are put into beaker, add excessive distilled water immersion 24h, stir resin, remove the broken resin of suspension; Then, the about 3h of HCl immersion with 2mol/L is washed till pH5～6 with distilled water, uses the NaOH of 2mol/L to soak 3h again, is washed till pH5～6 with distilled water, and the HCl with 2mol/L does the processing that makes the transition at last, is washed till neutrality with distilled water.
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| CN103787939A (en) * | 2014-03-12 | 2014-05-14 | 广州博采生物科技有限公司 | Method and device of separating and extracting amino acid products from protein hydrolysates |
| CN107417750A (en) * | 2017-05-16 | 2017-12-01 | 河南科技学院 | A kind of method that CAMP is extracted from microbial fermentation solution |
| CN115088847A (en) * | 2022-07-13 | 2022-09-23 | 呼伦贝尔市林海森林经营管理有限公司 | Method for extracting multiple amino acids from birch juice |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103787939A (en) * | 2014-03-12 | 2014-05-14 | 广州博采生物科技有限公司 | Method and device of separating and extracting amino acid products from protein hydrolysates |
| CN103787939B (en) * | 2014-03-12 | 2016-03-30 | 广州博采生物科技有限公司 | A kind of method of separation and Extraction amino acid products from protein hydrolyzate and equipment thereof |
| CN107417750A (en) * | 2017-05-16 | 2017-12-01 | 河南科技学院 | A kind of method that CAMP is extracted from microbial fermentation solution |
| CN107417750B (en) * | 2017-05-16 | 2020-04-21 | 河南科技学院 | Method for extracting cyclic adenosine monophosphate from microbial fermentation liquid |
| CN115088847A (en) * | 2022-07-13 | 2022-09-23 | 呼伦贝尔市林海森林经营管理有限公司 | Method for extracting multiple amino acids from birch juice |
| CN115088847B (en) * | 2022-07-13 | 2023-12-22 | 呼伦贝尔市林海森林经营管理有限公司 | Method for extracting multiple amino acids from birch juice |
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