CN102258793A - Application of core-shell nano particle - Google Patents
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- CN102258793A CN102258793A CN 201110196342 CN201110196342A CN102258793A CN 102258793 A CN102258793 A CN 102258793A CN 201110196342 CN201110196342 CN 201110196342 CN 201110196342 A CN201110196342 A CN 201110196342A CN 102258793 A CN102258793 A CN 102258793A
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Abstract
The invention provides an application of a core-shell nano particle, used for preparing a biological developer, a molecular biological probe and a targeting drug-loading carrier. The inner core material of the core-shell nano particle is a gold nano rod, the intermediate layer is silica and surface receptor, and the outer case is a couplet of a water based CdTe quantum dot and a biological molecule. The core-shell nano particle has low toxicity and low cost, solves the problem of water solubility of the nano particle and improves the biological compatibility of a compound nano particle. The core-shell nano particle has strong fluorescent characteristic, the surface receptor has a spectrum signal, and a fluorescent signal is observed by virtue of a biological imaging system to obtain information of a focus; the core-shell nano particle can enter into a cell of an organism to be combined as the biological probe by utilizing the strong fluorescent characteristic of the core-shell nano particle so as to carrying out early diagnosis on disease; and a compound of drug and the core-shell nano particle can be successfully resided on the surface of a cancer cell, and the loaded drug reaches the cancer cell, thus treatment effect is achieved.
Description
Technical field
The invention belongs to the particulate application of a kind of composite nano, be specifically related to a kind of application of nm-class core-and-shell particles.
Background technology
Nano-particle is meant the molecule of particle diameter in 1 to 100 nanometer range, because material presented under this yardstick characteristic and functions such as peculiar magnetic, electrical, optical are developed rapidly the research of nano-particle and application.Especially more extensive at bio-imaging and detection application, in order to reach as fluorescently-labeled biologic applications, it is very necessary preparing semiconductor-quantum-point water-soluble, stable good dispersion.
Gold nanorods has advantageous properties such as high stability, hypotoxicity, its excitation spectrum of II-VI compound semiconductor quantum dot is in visible-range, but and wavelength continuous distribution, it can be under same excitation source, the of the same race crystalline quantum dot that the simultaneous excitation size is different, can obtain the emission spectra of different visible lights, carry out the fluoroscopic examination of polynary series.People such as Peng (Peng X.G., SchLamp M.C., Kadavanich A.V., EpitaxiaL growth of highLyLuminescent CdSe/CdS core/sheLL nanocrystaLs with photostabiLity and eLectronic accessibiLity, J.Am.Chem.Soc.1997,119 (30): 7019-7029.) make raw material with Cd (CH3) 2 organo-metallic compounds such as grade, the CdSe/CdS or the CdSe/ZnS quantum dot that make core/shell structure at the CdS or the ZnS of CdSe surface coverage suitable thickness, but, though the core-shell type quantum point monodispersity that obtains is good, stability is strong, the quantum yield height, but spectral signal is not strong, is difficult for carrying out biological detection yet.The oiliness quantum dot needs chloroform dissolving just can transfer aqueous to could to combine with biomolecule, and the aqueous quantum dot can centrifugal back just can directly combine with biomolecule and save loaded down with trivial details switch process.The present invention utilizes the aqueous quantum dot that gold nanorods is wrapped up the compound nano-particle of preparation layer by layer, and this invention does not appear in the newspapers.
Summary of the invention
In order to solve the problem that prior art exists, the invention provides a kind of application of nm-class core-and-shell particles.
One, the invention provides a kind of application of nm-class core-and-shell particles, described a kind of nm-class core-and-shell particles is used to prepare the carrier of biological developer, molecular biosciences probe or target drug-carrying.
The application of described a kind of nm-class core-and-shell particles is characterized in that, the usage of biological developer is as follows: with the nm-class core-and-shell particles pure level of liquid chromatograph water dissolution, concentration is modulated to 6mM, is prepared into biological developer; Get the biological developer vein of 100uL and inject, utilize nm-class core-and-shell particles to have strong fluorescent characteristic, surface receptor has spectral signal, by bio-imaging systematic observation fluorescence signal, obtains the information of focus.
The application of described a kind of nm-class core-and-shell particles, it is characterized in that, the usage of molecular biosciences probe is as follows: utilize hydrophilic Measurement of Biopolymer Chitosan (chitosan) and the hud typed nano-particle parcel preparation molecular biosciences probe that has prepared, utilize the strong fluorescent characteristic of hud typed nano-particle can enter the organism inner cell in conjunction with as bioprobe disease being carried out early diagnosis; Wherein, nm-class core-and-shell particles and chitosan are used the pure level of liquid chromatograph water dissolution respectively, the concentration of nano-particle is 40mg/mL; Chitosan and hud typed nano-particle mass ratio are 1: 1, inject 2mL at every turn;
The application of described a kind of nm-class core-and-shell particles, it is characterized in that, the usage of the carrier of target drug-carrying is as follows: dexamethasone (PLGA) or folic acid (folate acid) are used for wrapping up hud typed nano-particle, utilize the strong fluorescent properties of hud typed nano-particle to carry out bio-imaging to the nano-particle behind the medicine parcel; Wherein, dexamethasone or the folic acid pure level of liquid chromatograph water dissolution, the concentration of nano-particle is 40mg/mL; By medicine and hud typed nano-particle mass ratio is 1: 1, makes the complex of medicine and nm-class core-and-shell particles, adopts intravenous mode to inject the complex of medicine and nm-class core-and-shell particles, injects 5mL at every turn; Success will follow resides in the cancerous cell surface for the complex of medicine and nm-class core-and-shell particles; Can observe around tumor cell has fluorescence signal, obtains information such as tumor size and position simultaneously; Perhaps, medicine carrying arrives at cancerous cell and reaches therapeutic effect.
Two, described a kind of hud typed nano-particle is seen accompanying drawing 1, and described nm-class core-and-shell particles is that inner nuclear material is a gold nanorods; The intermediate layer is silicon dioxide and surface receptor; Shell is the couplet of aqueous CdTe quantum dot and biomolecule; Described gold nanorods length is 50nm, and draw ratio is 2-3: 1; The thickness of the intermediate layer silicon dioxide of described parcel gold nanorods is 2-10nm, and the spacing of gold nanorods and silicon dioxide is controlled at 3-5nm; The surface receptor that introduce in the intermediate layer is 3-cyano group benzene sulfonyl chloride, parallel sulfhydryl amino benzene (pABT), isothiocyanic acid fat (RBITC) or 2-naphthalene thiol (2-NAT); Utilize electric charge suction method mutually that surface receptor is attached on the gold nanorods; The couplet of described biomolecule has immunoglobulin IgG or 8 antibody (anti-CEA8).
Three, the preparation method of described a kind of nm-class core-and-shell particles, step and condition are as follows:
(1) inner nuclear material gold nanorods utilizes the preparation of seed growth synthetic method: with HAuCL
4Be raw material, with NaBH
4Be Reducing agent, at first reduce Au
3+, obtain the gold seeds that diameter is 3-4nm, HAuC
L4With NaBH
4Mol ratio be 50: 0.02; With Ag
+Be assisting ion, add AgNO
3, HAuC
L4With AgNO
3Mol ratio be 50: 4; Adding the cetyl ammonium bromide is surfactant, HAuCL
4With the mol ratio of cetyl ammonium bromide be 50: 0.04; AgNO
3Add all with six alkyl ammonium bromide that to put into temperature after the gold seeds solution be 33 ℃ water bath, obtain gold nanorods after 24 hours;
(2) wrap up gold nanorods with silicon dioxide:
The cetyl ammonium bromide of the gold nanorods surface layer that obtains with centrifuging flush away step (1), gold nanorods after centrifugal adds the pure level of liquid chromatograph water, the gold nanorods concentration of aqueous solution is modulated to 2mM, again it being added concentration is in the ethanol ethyl silicate solution of 10mM, the volume ratio of described gold nanorods solution and described ethanol ethyl silicate solution is 1: 0.2-0.8, transferring pH value is 9, stirred 20 seconds and wait for 30 minutes as one-period, repeat 2-3 cycle, left standstill 24 hours, and obtained gold nanorods/silicon dioxide composite nanometer particle;
(3) surface receptor is introduced: utilize electric charge to inhale method mutually, under the room temperature, surface receptor is dissolved in to regulate concentration in the pure level of the liquid chromatograph water be 2mM, the prepared concentration of implantation step (2) is in silicon dioxide/gold nanorods composite nanometer particle solution of 6mM, the mol ratio of surface receptor and silicon dioxide/gold nanorods is 2: 3, stir, react 40-60 second, surface receptor is attached on silicon dioxide/gold nanorods; The surface receptor that introduce in the intermediate layer is 3-cyano group benzene sulfonyl chloride, parallel sulfhydryl amino benzene, isothiocyanic acid fat or 2-naphthalene thiol;
(4) utilize the water legal system to be equipped with aqueous CdTe quantum dot: with Te powder and NaBH
4Mixture is as presoma, Te and NaBH
4Mol ratio is 0.6: 2.5; Mercaptopropionic acid is a stabilizing agent, and mercaptopropionic acid and Caddy (Cleary) mixed liquor are reactant; Wherein, Te: mercaptopropionic acid: CdCL
2Mol ratio is 0.3: 2.5: 1, and reaction temperature is 100 ℃, and the response time is 1-30 hour, obtains aqueous CdTe quantum dot;
(5) combining of the gold nanorods of aqueous CdTe quantum dot and the silicon dioxide parcel of introducing surface receptor: by the mol ratio of aqueous CdTe quantum dot and IgG antibody is to prepare the mixed solution of aqueous quantum dot and IgG antibody at 60: 1; The gold nanorods of described mixed solution with the silicon dioxide parcel of introducing surface receptor mixed, and the mol ratio of described gold nanorods and aqueous CdTe quantum dot is 1: 20, and room temperature reacted 30-60 minute down, obtained a kind of nm-class core-and-shell particles.
Beneficial effect: the invention provides a kind of application of nm-class core-and-shell particles.Described hud typed nano-particle is that inner nuclear material is a gold nanorods; The intermediate layer is silicon dioxide and surface receptor; Shell is the couplet of aqueous CdTe quantum dot and biomolecule.The present invention is that the nanoparticle that will have magneto-electric behavior wraps up layer by layer, utilizes microcosmic composite algorithm water synthesising stability greater than 6 months, quantum yield up to 60%, toxicity is little, cost is low, nm-class core-and-shell particles.Well solved the water solublity problem of nanoparticle.
Can control the thickness of silicon dioxide by the amount that changes silester (TEOS), thereby reach the purpose of better control silicon dioxide and gold nanorods spacing.In the given ratio range of the present invention, can be controlled at 2-10nm, the spacing of gold nanorods and silicon dioxide is controlled at 3-5nm.
When preparing silicon dioxide layer with the stable gold nanorods of parcel CTAB such as APTMS, MPTMS and PVP, the silicon dioxide layer that the gold nanorods surface forms is uneven.The nano-particle that can obtain to wrap up the homogeneous texture compactness of the present invention, improve the optical characteristics and the magnetic characteristic of nano material simultaneously, each interfacial thickness all can reach 3-5nm, and magnetoelectricity sensitivity improves 10%, has further improved the bio-compatibility of composite nanometer particle.
A kind of nm-class core-and-shell particles of the present invention is applied to prepare the carrier of biological developer, molecular biosciences probe or target drug-carrying.
Utilize nm-class core-and-shell particles to have strong fluorescent characteristic, surface receptor has spectral signal, by bio-imaging systematic observation fluorescence signal, obtains the information of focus.
Utilize the strong fluorescent characteristic of hud typed nano-particle can enter the organism inner cell in conjunction with disease being carried out early diagnosis as bioprobe.
Success will follow resides in the cancerous cell surface for the complex of medicine and nm-class core-and-shell particles; Can observe around tumor cell has fluorescence signal, obtains information such as tumor size and position simultaneously; Perhaps, medicine carrying arrives at cancerous cell and reaches therapeutic effect.
Description of drawings
Fig. 1 is the structural representation of the nano-particle of a kind of nucleocapsid structure provided by the invention.The grain particle diameter can be controlled in several to tens nanometers.
Fig. 2 is a silicon dioxide parcel gold nanorods transmission electron microscope picture provided by the invention.
Fig. 3 is the emission spectra figure of the nano-particle of a kind of nucleocapsid structure provided by the invention.
Fig. 4 is the emission spectra figure of the nano-particle of the nucleocapsid structure that different preparation times obtain among the embodiment provided by the invention.
The specific embodiment
The method for making of 1 one kinds of nm-class core-and-shell particles of embodiment, step and condition are as follows:
Described a kind of hud typed nano-particle is seen accompanying drawing 1, and described nm-class core-and-shell particles is that inner nuclear material is a gold nanorods; The intermediate layer is silicon dioxide and surface receptor; Shell is the couplet of aqueous CdTe quantum dot and biomolecule; Described gold nanorods length is 50nm, and draw ratio is 2-3: 1; The thickness of the intermediate layer silicon dioxide of described parcel gold nanorods is 2-10nm, and the spacing of gold nanorods and silicon dioxide is controlled at 3-5nm; The surface receptor that introduce in the intermediate layer is 3-cyano group benzene sulfonyl chloride, parallel sulfhydryl amino benzene (pABT), isothiocyanic acid fat (RBITC) or 2-naphthalene thiol (2-NAT); Utilize electric charge suction method mutually that surface receptor is attached on the gold nanorods; The couplet of described biomolecule has immunoglobulin IgG or 8 antibody (anti-CEA8).
(1) inner nuclear material gold nanorods utilizes the preparation of seed growth synthetic method: with HAuCL
4Be raw material, with NaBH
4Be Reducing agent, at first reduce Au
3+, obtain the gold seeds that diameter is 3-4nm, HAuC
L4With NaBH
4Mol ratio be 50: 0.02; With Ag
+Be assisting ion, add AgNO
3, HAuC
L4With AgNO
3Mol ratio be 50: 4; Adding the cetyl ammonium bromide is surfactant, HAuCL
4With the mol ratio of cetyl ammonium bromide be 50: 0.04; AgNO
3Add all with six alkyl ammonium bromide that to put into temperature after the gold seeds solution be 33 ℃ water bath, obtain gold nanorods after 24 hours;
(2) wrap up gold nanorods with silicon dioxide:
The cetyl ammonium bromide of the gold nanorods surface layer that obtains with centrifuging flush away step (1), gold nanorods after centrifugal adds the pure level of liquid chromatograph water, the gold nanorods concentration of aqueous solution is modulated to 2mM, again it being added concentration is in the ethanol ethyl silicate solution of 10mM, the volume ratio of described gold nanorods solution and described ethanol ethyl silicate solution is 1: 0.2, transferring pH value is 9, stirred 20 seconds and wait for 30 minutes as one-period, repeat 2-3 cycle, left standstill 24 hours, and obtained gold nanorods/silicon dioxide composite nanometer particle;
(3) surface receptor is introduced: utilize electric charge to inhale method mutually, under the room temperature, surface receptor is dissolved in to regulate concentration in the pure level of the liquid chromatograph water be 2mM, the prepared concentration of implantation step (2) is in silicon dioxide/gold nanorods composite nanometer particle solution of 6mM, the mol ratio of surface receptor and silicon dioxide/gold nanorods is 2: 3, stir, reacted 40 seconds, surface receptor is attached on silicon dioxide/gold nanorods; The surface receptor that introduce in the intermediate layer is a 3-cyano group benzene sulfonyl chloride;
(4) utilize the water legal system to be equipped with aqueous CdTe quantum dot: with Te powder and NaBH
4Mixture is as presoma, Te and NaBH
4Mol ratio is 0.6: 2.5; Mercaptopropionic acid is a stabilizing agent, and mercaptopropionic acid and Caddy (Cleary) mixed liquor are reactant; Wherein, Te: mercaptopropionic acid: CdCL
2Mol ratio is 0.3: 2.5: 1, and reaction temperature is 100 ℃, and the response time is 1 hour, obtains aqueous CdTe quantum dot;
(5) combining of the gold nanorods of aqueous CdTe quantum dot and the silicon dioxide parcel of introducing surface receptor: by the mol ratio of aqueous CdTe quantum dot and IgG antibody is to prepare the mixed solution of aqueous quantum dot and IgG antibody at 60: 1; The gold nanorods of described mixed solution with the silicon dioxide parcel of introducing surface receptor mixed, and the mol ratio of described gold nanorods and aqueous CdTe quantum dot is 1: 20, and room temperature reacted 30 minutes down, obtained a kind of nm-class core-and-shell particles.
The method for making of 2 one kinds of nm-class core-and-shell particles of embodiment, the volume ratio of described gold nanorods solution of step (2) and described ethanol ethyl silicate solution is 1: 0.3; The described reaction of step (3) is 50 seconds, and the surface receptor that introduce in the intermediate layer is parallel sulfhydryl amino benzene; The described response time of step (4) is 2 hours; The described room temperature of step (5) reacted 40 minutes down; Remaining is with embodiment 1.
The method for making of 3 one kinds of nm-class core-and-shell particles of embodiment, the volume ratio of described gold nanorods solution of step (2) and described ethanol ethyl silicate solution is 1: 0.4; The described reaction of step (3) is 60 seconds, and the surface receptor that introduce in the intermediate layer is an isothiocyanic acid fat; The described response time of step (4) is 6 hours; The described room temperature of step (5) reacted 45 minutes down; Remaining is with embodiment 1.
The method for making of 4 one kinds of nm-class core-and-shell particles of embodiment, the volume ratio of described gold nanorods solution of step (2) and described ethanol ethyl silicate solution is 1: 0.6; The described reaction of step (3) is 60 seconds, and the surface receptor that introduce in the intermediate layer is the 2-naphthalene thiol; The described response time of step (4) is 8 hours; The described room temperature of step (5) reacted 50 minutes down; Remaining is with embodiment 1.
The method for making of 5 one kinds of nm-class core-and-shell particles of embodiment, the volume ratio of described gold nanorods solution of step (2) and described ethanol ethyl silicate solution is 1: 0.8; The described reaction of step (3) is 40 seconds, and the surface receptor that introduce in the intermediate layer is a 3-cyano group benzene sulfonyl chloride; The described response time of step (4) is 24 hours; The described room temperature of step (5) reacted 60 minutes down; Remaining is with embodiment 1.
The method for making of 6 one kinds of nm-class core-and-shell particles of embodiment, the volume ratio of described gold nanorods solution of step (2) and described ethanol ethyl silicate solution is 1: 0.5; The described reaction of step (3) is 50 seconds, and the surface receptor that introduce in the intermediate layer is a 3-cyano group benzene sulfonyl chloride; The described response time of step (4) is 30 hours; The described room temperature of step (5) reacted 30 minutes down; Remaining is with embodiment 1.
The application of 7 one kinds of nm-class core-and-shell particles of embodiment
A kind of nm-class core-and-shell particles of the present invention is used to prepare the carrier of biological developer, molecular biosciences probe or target drug-carrying;
Described a kind of hud typed nano-particle is seen accompanying drawing 1, and described nm-class core-and-shell particles is that inner nuclear material is a gold nanorods; The intermediate layer is silicon dioxide and surface receptor; Shell is the couplet of aqueous CdTe quantum dot and biomolecule; Described gold nanorods length is 50nm, and draw ratio is 2-3: 1; The thickness of the intermediate layer silicon dioxide of described parcel gold nanorods is 2-10nm, and the spacing of gold nanorods and silicon dioxide is controlled at 3-5nm; The surface receptor that introduce in the intermediate layer is 3-cyano group benzene sulfonyl chloride, parallel sulfhydryl amino benzene (pABT), isothiocyanic acid fat (RBITC) or 2-naphthalene thiol (2-NAT); Utilize electric charge suction method mutually that surface receptor is attached on the gold nanorods; The couplet of described biomolecule has immunoglobulin IgG or 8 antibody (anti-CEA8).
The application of described a kind of nm-class core-and-shell particles is characterized in that, the usage of biological developer is as follows: with the nm-class core-and-shell particles pure level of liquid chromatograph water dissolution, concentration is modulated to 6mM, is prepared into biological developer; Get the biological developer vein of 100uL and inject, utilize nm-class core-and-shell particles to have strong fluorescent characteristic, surface receptor has spectral signal, by bio-imaging systematic observation fluorescence signal, obtains the information of focus.
The application of described a kind of nm-class core-and-shell particles, it is characterized in that, the usage of molecular biosciences probe is as follows: utilize hydrophilic Measurement of Biopolymer Chitosan (chitosan) and the hud typed nano-particle parcel preparation molecular biosciences probe that has prepared, utilize the strong fluorescent characteristic of hud typed nano-particle can enter the organism inner cell in conjunction with as bioprobe disease being carried out early diagnosis; Wherein, nm-class core-and-shell particles and chitosan are used the pure level of liquid chromatograph water dissolution respectively, the concentration of nano-particle is 40mg/mL; Chitosan and hud typed nano-particle mass ratio are 1: 1, inject 2mL at every turn.
The application of described a kind of nm-class core-and-shell particles, it is characterized in that, the usage of the carrier of target drug-carrying is as follows: dexamethasone (PLGA) or folic acid (folate acid) are used for wrapping up hud typed nano-particle, utilize the strong fluorescent properties of hud typed nano-particle to carry out bio-imaging to the nano-particle behind the medicine parcel; Wherein, dexamethasone or the folic acid pure level of liquid chromatograph water dissolution, the concentration of nano-particle is 40mg/mL; By medicine and hud typed nano-particle mass ratio is 1: 1, makes the complex of medicine and nm-class core-and-shell particles, adopts intravenous mode to inject the complex of medicine and nm-class core-and-shell particles, injects 5mL at every turn; Success will follow resides in the cancerous cell surface for the complex of medicine and nm-class core-and-shell particles; Can observe around tumor cell has fluorescence signal, obtains information such as tumor size and position simultaneously; Perhaps, medicine carrying arrives at cancerous cell and reaches therapeutic effect.
Claims (4)
1. the application of a nm-class core-and-shell particles is characterized in that, it is used to prepare biological developer, molecular biosciences probe and target drug-carrying carrier; Described nm-class core-and-shell particles is that inner nuclear material is a gold nanorods; The intermediate layer is silicon dioxide and surface receptor; Shell is the couplet of aqueous CdTe quantum dot and biomolecule; Described gold nanorods length is 50nm, and draw ratio is 2-3: 1; The thickness of the intermediate layer silicon dioxide of described parcel gold nanorods is 2-10nm, and the spacing of gold nanorods and silicon dioxide is controlled at 3-5nm; The surface receptor that introduce in the intermediate layer is 3-cyano group benzene sulfonyl chloride, parallel sulfhydryl amino benzene, isothiocyanic acid fat or 2-naphthalene thiol; Utilize electric charge suction method mutually that surface receptor is attached on the gold nanorods; The couplet of described biomolecule has immunoglobulin or 8 antibody.
2. the application of a kind of nm-class core-and-shell particles as claimed in claim 1 is characterized in that, the usage of biological developer is as follows: with the nm-class core-and-shell particles pure level of liquid chromatograph water dissolution, concentration is modulated to 6mM, is prepared into biological developer; Get the biological developer vein of 100uL and inject, utilize nm-class core-and-shell particles to have strong fluorescent characteristic, surface receptor has spectral signal, by bio-imaging systematic observation fluorescence signal, obtains the information of focus.
3. the application of a kind of nm-class core-and-shell particles as claimed in claim 1, it is characterized in that, the usage of molecular biosciences probe is as follows: utilize hydrophilic Measurement of Biopolymer Chitosan (chitosan) and the hud typed nano-particle parcel preparation molecular biosciences probe that has prepared, utilize the strong fluorescent characteristic of hud typed nano-particle can enter the organism inner cell in conjunction with as bioprobe disease being carried out early diagnosis; Wherein, nm-class core-and-shell particles and chitosan are used the pure level of liquid chromatograph water dissolution respectively, the concentration of nano-particle is 40mg/mL; Chitosan and hud typed nano-particle mass ratio are 1: 1, inject 2mL at every turn.
4. the application of a kind of nm-class core-and-shell particles as claimed in claim 1, it is characterized in that, the usage of the carrier of target drug-carrying is as follows: dexamethasone (PLGA) or folic acid (folate acid) are used for wrapping up hud typed nano-particle, utilize the strong fluorescent properties of hud typed nano-particle to carry out bio-imaging to the nano-particle behind the medicine parcel; Wherein, dexamethasone or the folic acid pure level of liquid chromatograph water dissolution, the concentration of nano-particle is 40mg/mL; By medicine and hud typed nano-particle mass ratio is 1: 1, makes the complex of medicine and nm-class core-and-shell particles, adopts intravenous mode to inject the complex of medicine and nm-class core-and-shell particles, injects 5mL at every turn; Success will follow resides in the cancerous cell surface for the complex of medicine and nm-class core-and-shell particles; Can observe around tumor cell has fluorescence signal, obtains information such as tumor size and position simultaneously; Perhaps, medicine carrying arrives at cancerous cell and reaches therapeutic effect.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103271869A (en) * | 2013-04-08 | 2013-09-04 | 天津大学 | Preparation method of hollow polymer drug-loaded nano-rod |
| CN104028181A (en) * | 2014-04-24 | 2014-09-10 | 温州大学 | Precious metal/paramagnetic metal composite nanoparticle with core-shell structure and application thereof |
| CN106727274A (en) * | 2017-03-07 | 2017-05-31 | 常州大学 | A kind of preparation method of the polypyrrole/mesoporous silicon oxide/graphene quantum dot nano composite material of core shell structure |
| CN107550885A (en) * | 2017-07-31 | 2018-01-09 | 华中科技大学同济医学院附属协和医院 | It is a kind of to include nanoparticle vector of TLR3 parts and its preparation method and application in pattern recognition receptors |
| CN109821134A (en) * | 2019-01-18 | 2019-05-31 | 武汉大学 | A kind of three-dimensional network bioprobe and the preparation method and application thereof for living body |
| CN111888342A (en) * | 2020-07-02 | 2020-11-06 | 南方医科大学南方医院 | Drug-loaded nano-composite and preparation method and application thereof |
-
2011
- 2011-07-14 CN CN 201110196342 patent/CN102258793A/en active Pending
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| 《The Royal Society of Chemistry》 20100809 Qinghui Zeng et al. Au/SiO2 core/shell nanoparticles enhancing fluorescence resonance energy transfer efficiency in solution 第46卷, * |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103271869A (en) * | 2013-04-08 | 2013-09-04 | 天津大学 | Preparation method of hollow polymer drug-loaded nano-rod |
| CN104028181A (en) * | 2014-04-24 | 2014-09-10 | 温州大学 | Precious metal/paramagnetic metal composite nanoparticle with core-shell structure and application thereof |
| CN104028181B (en) * | 2014-04-24 | 2016-04-06 | 温州大学 | There is noble metal/paramagnetic metal composite nanoparticle and the application thereof of nucleocapsid structure |
| CN106727274A (en) * | 2017-03-07 | 2017-05-31 | 常州大学 | A kind of preparation method of the polypyrrole/mesoporous silicon oxide/graphene quantum dot nano composite material of core shell structure |
| CN107550885A (en) * | 2017-07-31 | 2018-01-09 | 华中科技大学同济医学院附属协和医院 | It is a kind of to include nanoparticle vector of TLR3 parts and its preparation method and application in pattern recognition receptors |
| CN107550885B (en) * | 2017-07-31 | 2021-08-13 | 华中科技大学同济医学院附属协和医院 | Nanoparticle carrier containing TLR3 ligand in pattern recognition receptor and preparation method and application thereof |
| CN109821134A (en) * | 2019-01-18 | 2019-05-31 | 武汉大学 | A kind of three-dimensional network bioprobe and the preparation method and application thereof for living body |
| CN111888342A (en) * | 2020-07-02 | 2020-11-06 | 南方医科大学南方医院 | Drug-loaded nano-composite and preparation method and application thereof |
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