CN102253200B - Enzyme-linked immuno sorbent assay (elisa) reader, elisa plate and machining method thereof - Google Patents
Enzyme-linked immuno sorbent assay (elisa) reader, elisa plate and machining method thereof Download PDFInfo
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- CN102253200B CN102253200B CN 201110177061 CN201110177061A CN102253200B CN 102253200 B CN102253200 B CN 102253200B CN 201110177061 CN201110177061 CN 201110177061 CN 201110177061 A CN201110177061 A CN 201110177061A CN 102253200 B CN102253200 B CN 102253200B
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Abstract
The invention discloses an enzyme-linked immuno sorbent assay (elisa) reader, an elisa plate and a machining method thereof, which aim to realize the temperature control of an ELISA experiment. In the elisa reader, a temperature sensor is arranged on the elisa plate and is electrically connected with a temperature routing inspection meter. The elisa plate is provided with the temperature sensor. The machining method comprises the following steps of: taking the elisa plate; punching at the position where which the temperature sensor is needed to be inserted; injecting paraffin oil into a hole; and arranging a printed circuit board (PCB) with the temperature sensor on the elisa plate, and inserting the temperature sensor into the hole into which the paraffin oil is injected.
Description
Technical field
The present invention relates to a kind of testing tool, relate in particular to a kind of microplate reader, ELISA Plate and job operation thereof of carrying out enzymatic activity and immunoassay.
Background technology
Microplate reader (Micro plate Reader) is the professional instrument that enzyme linked immunosorbent detection (EIA) experimental result is read and analyzes, and its core is a tintmeter, by colourimetry, analyzes the content of antigen or antibody.By being coupled at, enzymatic chromogenic substrate on antigen or antibody carries out enzyme linked immunoassay, and reaction result shows with color, and the depth by colour developing is the concentration that the size of absorbance just can judge antibody to be measured in sample or antigen.
In enzyme linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA), temperature controlled accuracy directly has influence on the accuracy of enzymatic activity and immunoassay result mensuration.Therefore, temperature how accurately to control microplate reader becomes a problem demanding prompt solution.
Summary of the invention
Fundamental purpose of the present invention is to disclose a kind of microplate reader, ELISA Plate and job operation thereof, to realize the temperature control to the ELISA experiment.
For reaching above-mentioned purpose, the present invention discloses a kind of microplate reader, and its ELISA Plate is provided with temperature sensor, and this temperature sensor and temperature polling instrument are electrically connected.Wherein ELISA Plate is the carrier of the interior adsorption antigen of microplate reader or antibody.
According to microplate reader of the present invention, to with the ELISA Plate of temperature sensor, be placed in microplate reader, close door, the extension line of temperature sensor is connected with temperature polling instrument, open the microplate reader power supply, set the temperature of microplate reader and wait for the stable of each temperature sensor reading, can obtain microplate reader and control situation in the temperature of ELISA Plate regional, and then realized accurately controlling each regional temperature of this ELISA Plate by the adjusting of microplate reader temperature, thereby also just can realize the temperature of ELISA experiment is controlled.
For reaching above-mentioned purpose, the present invention also discloses a kind of ELISA Plate, and it is provided with temperature sensor.
For reaching above-mentioned purpose, the present invention also discloses a kind of job operation of ELISA Plate, comprising:
Get ELISA Plate;
Will insert the position punching of temperature sensor;
Inject paraffin oil in hole;
To be placed on ELISA Plate with the pcb board of temperature sensor, and temperature sensor is inserted to the above-mentioned hole of injecting paraffin oil.
ELISA Plate disclosed in this invention and job operation thereof, simple, convenient, making and raw materials cost are all lower.Preferably, the present invention can measure each regional temperature of ELISA Plate by an array of temperature sensor and control situation, preferably, can adopt the DS18B20 temperature sensor of single wire digital to form an array of temperature sensor; For example, anyhow respectively three arrange formation one array of temperature sensor with 9 DS18B20 temperature sensors, and with all DS18B20 temperature sensors of same data bus parallel connection, the extension line of described data bus is 3, with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20, is connected respectively.So, on the one hand, adopt the DS18B20 digital temperature sensor of unibus, member is simple, and temperature resolution can reach 0.0625 degree; On the other hand, adopt the digital sensing technology, overcome the impact of the situations such as wiring on the temperature measuring accuracy; Moreover the extension line that is arranged so that array of temperature sensor of unibus only has 3, make the microplate reader closely to close door in the mensuration process, guaranteed the consistent of condition determination and actual service conditions.In addition, in this array of temperature sensor, can increase as required or reduce the quantity of temperature sensor, extendability is strong.
The accompanying drawing explanation
The structural representation that Fig. 1 is the disclosed microplate reader of the embodiment of the present invention;
The wiring diagram that Fig. 2 is the disclosed temperature sensing array of the embodiment of the present invention;
Figure 3 shows that the flow process chart of the disclosed ELISA Plate of the embodiment of the present invention.
Embodiment
Below in conjunction with Figure of description, specific embodiments of the invention are described in detail.
Embodiment mono-
The present embodiment discloses a kind of microplate reader, and as shown in Figure 1, the ELISA Plate 2 in this microplate reader 1 is provided with temperature sensor 3, and wherein this temperature sensor 3 is electrically connected with the temperature polling instrument 4 outside this microplate reader 1.
Wherein temperature polling instrument 4 is applicable to that multimetering shows and control temperature, generally can patrol and examine 1～64 drive test amount temperature signal, can touring detection and show the multi way temperature signal, with all kinds of temperature sensors, transmitter, be used in conjunction with.It should be noted that: in other embodiments, this temperature polling instrument 4 also can be integrated into an even functional module of this ELISA Plate 2 of this microplate reader 1, and this kind of conversion also belongs to protection scope of the present invention.In the present embodiment, temperature polling instrument 4 is placed in outside microplate reader, and its advantage is, the ELISA Plate that this microplate reader only need to be used existing microplate reader is improved and can be reached purpose of the present invention, and, when temperature polling instrument 4 or microplate reader go wrong, also be convenient to safeguard and process.
Because the panel of ELISA Plate has certain area, its hole count can be divided into 48 holes, 96 holes, generally commonly used is 96 holes, therefore single temperature sensor 1 is not enough to complete these ELISA Plate 3 each the regional temperature of feedback usually, and be necessary to arrange an array of temperature sensor, ELISA Plate central authorities and each peripheral zone is carried out to temperature detection.For this reason, it is example that the ELISA Plate in 96 holes is take in the present invention, with array of temperature sensor as shown in Figure 2, detects ELISA Plate central authorities and each peripheral regional temperature.
As shown in Figure 2, in the figure, this array of temperature sensor is formed in parallel by 9 DS18B20 temperature sensors 41～49.In the pcb board shown in Fig. 2, correspondence is distributed in 9 DS18B20 temperature sensors 41～49 on ELISA Plate and forms array of temperature sensor, the center of the corresponding ELISA Plate of its central temperature sensor with each three row anyhow; All DS18B20 temperature sensors are connected in parallel on the same data bus, the extension line of data bus is 3, with GND (ground connection) pin, VDD (supply voltage) pin and DQ (forms data bus) pin of the temperature sensor of each DS18B20, is connected.
In Fig. 2, this pcb board also is provided with single 3 needle sockets to connect this extension line and internal temperature sensor array.Preferably, these single 3 needle sockets are located at the central part on this narrow limit of ELISA Plate; This ELISA Plate is provided with the jack that inserts temperature sensor (if the position in the hole carried on existing ELISA Plate and size conform to size with the position of the temperature sensor of insertion in advance, the hole that can utilize this microplate reader to carry and omit the step of punching).
In the present embodiment, the DS18B20 temperature sensor of employing has following characteristics:
The temperature value that reflects device with the form of 9 bit digital quantity;
Unique one-wire interface only needs a port pinout to carry out communication (the DS18B20 temperature sensor sends or receives information by an one-wire interface, therefore between central microprocessor and DS1820, only needs a connecting line (adding ground wire));
Power supply for read-write and temperature transition can obtain from data line itself, without external power source;
Be convenient to the multiple spot distribution applications; Because each DS1820 has the sheet sequence number of a uniqueness, so many DS1820 can be connected in parallel on a single bus simultaneously, so just can be placed on many different places to temperature sensor;
℃-+125 ℃, temperature-measuring range-55, increase progressively with 0.1 ℃;
Temperature digital amount 200ms switching time;
The definable non-volatile temperature alarming setting of user;
The warning search command is identified and is indicated and surpasses program limiting temperature (temperature alarming condition).
Based on These characteristics, make the ELISA Plate shown in Fig. 2 and array of temperature sensor thereof at least have and following advantage:
On the one hand, high precision, high reliability, the DS18B20 digital temperature sensor of employing unibus, member is simple, and temperature resolution can reach 0.0625 degree;
On the other hand, adopt the digital sensing technology, overcome the impact of the situations such as wiring on the temperature measuring accuracy;
Moreover the extension line that is arranged so that array of temperature sensor of unibus only has 3, make the microplate reader closely to close door in the mensuration process, guaranteed the consistent of condition determination and actual service conditions.
In addition, in this array of temperature sensor, can increase as required or reduce the quantity of temperature sensor, extendability is strong.
It should be noted that in other embodiments, above-mentioned DS18B20 temperature sensor also can be substituted by other 1-wire digital temperature sensor, for example other device in DS18Bxx series.
Embodiment bis-
Corresponding with above-described embodiment one, the present embodiment discloses a kind of job operation of ELISA Plate, as shown in Figure 3, comprises the following steps:
Step S1, get ELISA Plate.
Step S2, will insert the position punching of temperature sensor.Usually, if carry porosely on the ELISA Plate in step S1, and the position in this hole conforms to the position of the pre-temperature sensor inserted, and omits the step of this punching.
Step S3, in hole, inject paraffin oil.Paraffin oil claims again mineral oil, is the potpourri from the resulting colorless and odorless of crude oil fractionation, has good closure.
Step S4, will be placed on ELISA Plate with the pcb board of temperature sensor, and temperature sensor is inserted to the above-mentioned hole of injecting paraffin oil.
Wherein, corresponding with Fig. 2, the circuit production of this pcb board comprises: with 9 DS18B20 temperature sensors, anyhow respectively three arrange formation one array of temperature sensor; With same data bus all DS18B20 temperature sensors in parallel, the extension line of data bus is 3, with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20, is connected respectively.Preferably, this pcb board also is provided with single 3 needle sockets to connect this extension line and internal temperature sensor array.When above-mentioned pcb board and ELISA Plate are carried out to contraposition, these single 3 needle sockets are located to the central part on this narrow limit of ELISA Plate, the center of the temperature sensor of these array of temperature sensor central authorities being inserted to described ELISA Plate.
The job operation of the disclosed ELISA Plate of the present embodiment, processing is simple, convenient, and cost is low.
Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although with reference to previous embodiment, the present invention is had been described in detail, those of ordinary skill in the art is to be understood that: its technical scheme that still can put down in writing aforementioned each embodiment is modified, or part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution break away from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (7)
1. an ELISA Plate, it is characterized in that, be provided with temperature sensor, described temperature sensor is 1-wire digital temperature sensor, and described 1-wire digital temperature sensor is the DS18B20 temperature sensor, and described ELISA Plate is rectangular, 9 described DS18B20 temperature sensors are distributed on described ELISA Plate, anyhow respectively three to arrange formation one array of temperature sensor, described ELISA Plate is provided with the jack that inserts described temperature sensor, and its central temperature sensor inserts the center of described ELISA Plate.
2. ELISA Plate according to claim 1, it is characterized in that, all DS18B20 temperature sensors are connected in parallel on the same data bus, and the extension line of described data bus is 3, with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20, are connected respectively.
3. ELISA Plate according to claim 2, is characterized in that, pcb board is provided with single 3 needle sockets and pegs graft to connect the temperature sensing array and be placed in the temperature polling instrument outside described ELISA Plate for described 3 extension lines; These single 3 needle socket correspondences are located at the central part on the narrow limit of described ELISA Plate.
4. a microplate reader, is characterized in that, its ELISA Plate is provided with temperature sensor, and this temperature sensor and temperature polling instrument are electrically connected.
5. microplate reader according to claim 4, is characterized in that, described temperature polling instrument is placed in outside described microplate reader;
Wherein said ELISA Plate is rectangular, described temperature sensor is the DS18B20 temperature sensor, 9 described DS18B20 temperature sensors are distributed on described ELISA Plate, and anyhow respectively three to arrange formation one array of temperature sensor, its central temperature sensor inserts the center of described ELISA Plate; And all DS18B20 temperature sensors are connected in parallel on the same data bus, the extension line of described data bus is 3, with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20, is connected respectively.
6. the job operation of an ELISA Plate, is characterized in that, comprising:
Get ELISA Plate;
Will insert the position punching of temperature sensor;
Inject paraffin oil in hole;
To be placed on ELISA Plate with the pcb board of temperature sensor, and temperature sensor is inserted to the above-mentioned hole of injecting paraffin oil.
7. the job operation of ELISA Plate according to claim 6, is characterized in that, the circuit production of described pcb board comprises:
Anyhow respectively three arrange formation one array of temperature sensor with 9 DS18B20 temperature sensors;
With same data bus all DS18B20 temperature sensors in parallel, the extension line of described data bus is 3, with GND pin, VDD pin and the DQ pin of the temperature sensor of each DS18B20, is connected respectively.
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| CN104729740A (en) * | 2015-02-26 | 2015-06-24 | 中国计量科学研究院 | Multi-channel wireless temperature measuring instrument and measurement method |
| CN109622077A (en) * | 2018-12-10 | 2019-04-16 | 郑州安图生物工程股份有限公司 | Self-enclosed reaction orifice plate and preparation method thereof when a kind of incubation |
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| US8079758B2 (en) * | 2007-10-25 | 2011-12-20 | Sebacs Co., Ltd. | Temperature computing instrument and method for calibrating temperature of sensor part used therefor |
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| CN2837841Y (en) * | 2005-10-31 | 2006-11-15 | 陕西科技大学 | Detector for quick detection of milk sample |
| CN101145060A (en) * | 2007-09-21 | 2008-03-19 | 北京工业大学 | Temperature-controlled array for micro-fluidic chip |
| CN201097243Y (en) * | 2007-09-21 | 2008-08-06 | 北京工业大学 | Temperature control array for mini-flow control chip |
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