CN102250218B - Method for hydrogen-bonding adsorption purification of oxytocin by using agarose comprising beta-cyclodextrin ligand - Google Patents
Method for hydrogen-bonding adsorption purification of oxytocin by using agarose comprising beta-cyclodextrin ligand Download PDFInfo
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Abstract
The invention relates to a method for chemically synthesizing oxytocin by using hydrogen-bonding adsorption chromatographic separation and purification. In the method, a high-concentration and high-crosslinking-degree agarose gel medium, on the surface of which beta-cyclodextrin ligand is bonded, is adopted; and the oxytocin is separated and purified from a rough oxytocin product which is obtained through chemical synthesis (comprising liquid-phase synthesis and solid-phase synthesis) according to a hydrogen-bonding adsorption principle. The method is used for producing oxytocin raw material medicines, has the advantages of high selectivity, high sample loading capacity, high medium recycling rate and the like; and the defects of low medium recycling rate and low sample loading capacity existing in the conventional reverse phase chromatography are overcome.
Description
Technical field
The present invention relates to a kind of chemically synthesized polypeptide medicine---the adsorption by hydrogen bond chromatographic separation and purification method of oxytocin, concrete the has been Ago-Gel medium of beta-cyclodextrin aglucon that adopted surface bond, utilizes adsorption by hydrogen bond chromatographic principles separation and purification chemosynthesis oxytocin.
Background technology
Nature exists huge, the miscellaneous biologically active peptides of quantity, relate to various toxin, hormone, antibacterial peptide, pheromone, cytokine etc., in various vital movements, play important regulating effect, the processes such as wide participation molecular recognition, signal transduction, enzyme adjusting alive, immunomodulatory, cytodifferentiation and ontogeny regulation and control.Since polypeptide synthetic technological invention at the beginning of last century, there have been tens kinds of artificial synthetic polypeptide medicaments listings, and adopted by extensive patients.Along with again and again breaking out in recent years food, Problems of Drug Safety, and the raising of people to drug quality and safety in utilization consciousness, the attention that the purity rubric that polypeptide drugs are produced and quality control are subject to the parties concerned again.
Oxytocin (oxytocin, OT) in 1895, be found, structure as shown in Figure 1, it is the nonapeptide acid amides with a disulfide linkage, have another name called pitocin, it is a kind of mammalian hormones, mainly the neurosecretion maxicell system secretion by necleus of hypothalamus,supraoptic and paraventricular nucleus produces, from posterior pituitary, be released into blood, the organ that arrives far-end along blood circulation plays a role, being widely used in clinically induced labor, hastening parturition and preventing the profuse bleeding causing because of minute puerperium uterine inertia, is that the unique approval of current U.S. FDA is for the medicine of clinical induced labor.Its molecular weight 1007.2Da, molecular formula C
43h
66n
12o
12s
2, iso-electric point 7.7, soluble in water, be dissolved in acetone, butanols and dilute acetic acid, be insoluble to ether and sherwood oil, dry product is stable, more stable in the acidic solution of pH3.8～4.4, unstable in basic solution.The production of medicinal oxytocin in one's early years adopts the mode of extracting from animal posterior pituitary tissue, has inactivation of virus, removes the safety issues such as anaphylactogen, depyrogenation, and in extract, exist difficult isolating construction to be similar to thing, be i.e. β-hypophamine.China is from 1969 for the first time since synthetic oxytocin, domestic 30Yu Jia oxytocin preparation manufacturer all adopt nonapeptide acid amides through sodium-liquid ammonia process for caustic soda purification eliminate, the way synthetic oxytocin of condensation, the purge process of the finished product is more complicated, product purity is low, yield is low, and this is also to cause one of clinical factor that occurs part untoward reaction.Major impurity in chemosynthesis oxytocin crude product, it is the assorted peptide of some and target polypeptides similar, the fracture peptide that the diastereomer that for example amino acid racemization produces, the disappearance peptide that partial amino-acid does not connect generation, peptide bond rupture produce, and the reduction-state open loop peptide that thoroughly do not produce of oxidation etc.
Traditional purification process of artificial synthetic polypeptide medicaments is multistep reverse-phase chromatography purifying, reverse-phase chromatography is the most much higher peptide purification method of current resolving power, therefore can accurately differentiate the difference of an amino-acid residue, except as purification process, also through being often used as final purity verification step.Although existing commercial preparation scale reverse-phase chromatographic column, but the large-scale production that reverse-phase chromatography technology is applied to polypeptide drugs also has not enough: first, the sample carrying capacity of RPLC is lower, especially more to impurity crude product, excessive rough sample can cause the irreversible collapse of chromatographic column; The second, medium kind is limited, is difficult for amplifying; The 3rd, medium to reuse number of times limited, the pre-treatment of sample is had relatively high expectations; The 4th, conventional silica matrix bonding alkyl aglucon, such chromatography separation media, as the production of clinical application, its biocompatibility and biological safety do not have the medium of dextran, agar carbohydrate high.
Ago-Gel medium is natural polysaecharides chromatographic media, is invented the sixties in 20th century, has the numerous characteristics of perfect medium: high cell size (90-96%), even also can keep high cell size under high density; The structure with the opening that connects fiber; High connectedness has effectively guaranteed material transfer in molecule; Bigger serface provides very high adsorptive power; Easily be prepared into the spheroidal particle of different agarose concentrations, different size to meet the demand of different field; The stable cleaning step in place that can meet harshness in sodium hydroxide; Low non-specific adsorption makes application surface wider; There is elasticity and non-brittle (more easily realizing recharging of chromatographic column), can make by appropriate cross-linking step again medium there is rigidity (having higher withstand voltage properties); Surface has a large amount of hydrophilic hydroxyl groups, can be replaced by difference in functionality aglucon and be applied to multiple adsorption chromatography technology.
Beta-cyclodextrin is a kind of polysaccharide molecule with barrel-like structure, structure as shown in Figure 2, inside has hydrophobic cavity, outside surface has abundant hydrophilic hydroxyl groups and ehter bond, can provide hydrogen bond to form site, mainly as pharmaceutical carrier, form inclusion compound with drug molecule, object is the wetting ability that increases drug molecule, improves solubleness and the rate of release of drug molecule in the recycle system.Itself also has certain chiral selectivity beta-cyclodextrin molecule.
Adsorption by hydrogen bond chromatogram is to utilize the power that forms hydrogen bond action between chromatographic media and target molecule to realize the separated novel chromatography separating method of a class, utilization is without the high density of any aglucon, high-crosslinking-degree sepharose itself as adsorption by hydrogen bond chromatographic media, and the research of the mechanism and use of separation and purification Chinese medicine polyphenolic compound or polypeptide has been reported.
Summary of the invention
The object of the invention is to adopt homemade can scale amplify take the sepharose adsorption by hydrogen bond chromatographic media that beta-cyclodextrin is aglucon, separation and purification chemosynthesis oxytocin crude product, simplify purifying process, improve target compound selectivity, product recovery rate, product purity and biological safety, improve sample carrying capacity and medium recycling degree.Present method is applicable to the chemosynthesis oxytocin crude product of the synthetic or solid phase synthesis of liquid phase.The object of the invention is to be achieved through the following technical solutions:
The invention provides a kind of synthetic method that cross-linked agarose gel is matrix, the beta-cyclodextrin of the take adsorption by hydrogen bond chromatographic media that is aglucon of take, comprise the steps:
1) 1 liter of the high-crosslinking-degree agarose gel microsphere of 12% concentration, 30 μ m median sizes, proceeds to reaction flask or reactor after washing with water;
2) add 100-200ml water, add 5-15g sodium sulfate, stir 2-3h;
3) add the sodium hydroxide of the 20-50% of 100-200ml water and 50-60ml, 60rpm stirs;
4) add 30-60ml epoxidation reagent, comprise epoxy chloropropane and BDO-diglycidylether, 20-30 ℃, 60rpm stirs, reaction 6-8h;
5) with hydrochloric acid, adjust pH 4-6;
6) add 20-50mg beta-cyclodextrin, the sodium hydroxide of 20-50%, 20-30 ℃, 60rpm stirs, reaction 12-20h;
7) with hydrochloric acid, adjust pH 4-6, wash 10 column volumes;
8) be stored in 20% ethanol standby.
A kind of method that the invention provides beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic separation and purification chemosynthesis oxytocin, concrete steps are as follows:
1) mobile phase composition: acetonitrile-acetic acid-aqueous systems, initial flow is 20-30% acetonitrile mutually, 0.45 μ m membrane filtration for the moving phase preparing, ultrasonic degas or vacuum outgas 10-30min, standing 0.5-1h is standby for room temperature;
2) chemosynthesis oxytocin dissolving crude product, in initial flow phase, is mixed with 10～50mg/mL solution, ultrasonic dissolution assisting 1～5min, 0.45 μ m membrane filtration;
3) beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic media dress post, with the initial flow sample introduction after 2 column volumes that balances each other, sample volume is column volume 5～50%;
4) type of elution: after 2 column volumes of initial flow equality wash-out, 2 column volumes of pure water isocratic elution, then adopt 0～10% acetic acid linear gradient elution, and gradient length is 5～10 column volumes, linear rate of flow 20～100cm/h;
5) UV-detector 280nm on-line monitoring, collects cut automatically;
6) by step 5) the cut lyophilize of collecting, with 15% acetonitrile (containing 0.1% trifluoroacetic acid), dissolve and be mixed with 0.2mg/mL solution, carry out purity detecting: adopt RPLC C18 post, adopting acetonitrile-trifluoroacetic acid-water is moving phase system, linear gradient elution, 15% acetonitrile (containing 0.1% trifluoroacetic acid) is gradient start, 90% acetonitrile (containing 0.1% trifluoroacetic acid) is gradient terminal, gradient length 30min, UV-detector 210nm on-line monitoring, calculates oxytocin purity and yield.
The invention provides a kind of method of using self-control beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic media separation and purification chemosynthesis oxytocin, that the useful of existing polypeptide separation purification method supplemented, the advantage of comparing with traditional multistep reverse-phase chromatography technology is: the separated polypeptide that obtains higher degree of a step, and the rate of recovery is high; Medium is strong to crude product tolerance, and sample carrying capacity is high, and medium can reuse tens of times, and the scale that is easy to is amplified; Biocompatibility and the biological safety of medium are high.
Accompanying drawing explanation
Fig. 1 oxytocin molecular structure
Fig. 2 beta-cyclodextrin molecular structure
In Fig. 3 embodiment 1, adopt beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic media separation and purification liquid phase synthetic oxytocin crude product color atlas
In Fig. 4 embodiment 2, adopt beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic media separation and purification solid phase synthesis oxytocin crude product color atlas
Embodiment
Embodiment 1, beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic media separation and purification liquid phase synthetic oxytocin crude product (initial purity approximately 17%)
1) mobile phase composition: acetonitrile-acetic acid-aqueous systems, initial flow is 30% acetonitrile mutually, 0.45 μ m membrane filtration for the moving phase preparing, ultrasonic degas or vacuum outgas 20min, standing 0.5h is standby for room temperature;
2) chemosynthesis oxytocin dissolving crude product, in initial flow phase, is mixed with 50mg/mL solution, ultrasonic dissolution assisting 5min, 0.45 μ m membrane filtration;
3) beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic media dress post, post bed diameter 10mm, post bed height 30cm, with the initial flow sample introduction after 2 column volumes that balances each other, sample volume is column volume 10%;
4) type of elution: after 2 column volumes of initial flow equality wash-out, 2 column volumes of pure water isocratic elution, then adopt 0～10% acetic acid linear gradient elution, and gradient length is 10 column volumes, linear rate of flow 40cm/h;
5) UV-detector 280nm on-line monitoring, collects cut automatically;
6) by step 5) the cut lyophilize of collecting, with 15% acetonitrile (containing 0.1% trifluoroacetic acid), dissolve and be mixed with 0.2mg/mL solution, carry out purity detecting: adopt RPLC C18 post, adopting acetonitrile-trifluoroacetic acid-water is moving phase system, linear gradient elution, 15% acetonitrile (containing 0.1% trifluoroacetic acid) is gradient start, 90% acetonitrile (containing 0.1% trifluoroacetic acid) is gradient terminal, gradient length 30min, UV-detector 210nm on-line monitoring;
7), after purifying, oxytocin purity is increased to 96% by 17%, the rate of recovery 78%.
3) mobile phase composition: acetonitrile-acetic acid-aqueous systems, initial flow is 20% acetonitrile mutually, 0.45 μ m membrane filtration for the moving phase preparing, ultrasonic degas or vacuum outgas 20min, standing 0.5h is standby for room temperature;
4) chemosynthesis oxytocin dissolving crude product, in initial flow phase, is mixed with 50mg/mL solution, ultrasonic dissolution assisting 3min, 0.45 μ m membrane filtration;
3) beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic media dress post, post bed diameter 16mm, post bed height 30cm, with the initial flow sample introduction after 2 column volumes that balances each other, sample volume is column volume 20%;
4) type of elution: after 2 column volumes of initial flow equality wash-out, 2 column volumes of pure water isocratic elution, then adopt 0～10% acetic acid linear gradient elution, and gradient length is 10 column volumes, linear rate of flow 30cm/h;
5) UV-detector 280nm on-line monitoring, collects cut automatically;
6) by step 5) the cut lyophilize of collecting, with 15% acetonitrile (containing 0.1% trifluoroacetic acid), dissolve and be mixed with 0.2mg/mL solution, carry out purity detecting: adopt RPLC C18 post, adopting acetonitrile-trifluoroacetic acid-water is moving phase system, linear gradient elution, 15% acetonitrile (containing 0.1% trifluoroacetic acid) is gradient start, 90% acetonitrile (containing 0.1% trifluoroacetic acid) is gradient terminal, gradient length 30min, UV-detector 210nm on-line monitoring;
7), after purifying, oxytocin purity is increased to 98% by 58%, the rate of recovery 85%.
Claims (2)
1. a method for the sepharose adsorption by hydrogen bond chromatographic media separation and purification chemosynthesis oxytocin that beta-cyclodextrin is aglucon is take in employing, and separation purification method is specific as follows:
1) mobile phase composition: acetonitrile-acetic acid-aqueous systems, initial flow is 20-30% acetonitrile mutually, 0.45 μ m membrane filtration for the moving phase preparing, ultrasonic degas or vacuum outgas 10-30min, standing 0.5-1h is standby for room temperature;
2) chemosynthesis oxytocin dissolving crude product, in initial flow phase, is mixed with 10～50mg/mL solution, ultrasonic dissolution assisting 1～5min, 0.45 μ m membrane filtration;
3) beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic media dress post, with the initial flow sample introduction after 2 column volumes that balances each other, sample volume is column volume 5～50%;
4) type of elution: after 2 column volumes of initial flow equality wash-out, 2 column volumes of pure water isocratic elution, then adopt 0～10% acetic acid linear gradient elution, and gradient length is 5～10 column volumes, linear rate of flow 20～100cm/h;
5) UV-detector 280nm on-line monitoring, collects cut automatically;
6) by step 5) the cut lyophilize of collecting, with 15% acetonitrile containing 0.1% trifluoroacetic acid, dissolve and be mixed with 0.2mg/mL solution, carry out purity detecting: adopt RPLC C18 post, adopting acetonitrile-trifluoroacetic acid-water is moving phase system, linear gradient elution, and 15% acetonitrile that contains 0.1% trifluoroacetic acid is gradient start, 90% acetonitrile containing 0.1% trifluoroacetic acid is gradient terminal, gradient length 30min, UV-detector 210nm on-line monitoring, calculates oxytocin purity and yield;
The step of preparing described beta-cyclodextrin genin and agarose gel adsorption by hydrogen bond chromatographic media comprises:
1) 1 liter of the high-crosslinking-degree agarose gel microsphere of 12% concentration, 30 μ m median sizes, proceeds to reaction flask or reactor after washing with water;
2) add 100-200ml water, add 5-15g sodium sulfate, stir 2-3h;
3) add the sodium hydroxide of the 20-50% of 100-200ml water and 50-60ml, 60rpm stirs;
4) add 30-60ml epoxidation reagent, comprise epoxy chloropropane and BDO-diglycidylether, 20-30 ℃, 60rpm stirs, reaction 6-8h;
5) with hydrochloric acid, adjust pH4-6;
6) add 20-50mg beta-cyclodextrin, the sodium hydroxide of 20-50%, 20-30 ℃, 60rpm stirs, reaction 12-20h;
7) with hydrochloric acid, adjust pH4-6, wash 10 column volumes;
8) be stored in 20% ethanol standby.
2. method according to claim 1, is characterized in that, described chemosynthesis oxytocin comprises that liquid phase is synthesized and the oxytocin of solid phase synthesis.
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| US4539399A (en) * | 1984-07-27 | 1985-09-03 | Advanced Separation Technologies Inc. | Bonded phase material for chromatographic separations |
| CN101456898A (en) * | 2007-12-10 | 2009-06-17 | 中国科学院过程工程研究所 | Method for separating and purifying polypeptide by using hydrogen bond adsorption chromatogram |
| CN101955514A (en) * | 2009-07-14 | 2011-01-26 | 杭州华锦药业股份有限公司 | Method for synthesizing agarose gel hydrogen bond adsorbing chromatography medium by using quercetin as genin |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4539399A (en) * | 1984-07-27 | 1985-09-03 | Advanced Separation Technologies Inc. | Bonded phase material for chromatographic separations |
| CN101456898A (en) * | 2007-12-10 | 2009-06-17 | 中国科学院过程工程研究所 | Method for separating and purifying polypeptide by using hydrogen bond adsorption chromatogram |
| CN101955514A (en) * | 2009-07-14 | 2011-01-26 | 杭州华锦药业股份有限公司 | Method for synthesizing agarose gel hydrogen bond adsorbing chromatography medium by using quercetin as genin |
Non-Patent Citations (3)
| Title |
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| 利用β-环糊精键合固定相分离纯化葛根素;贺湘凌 等;《色谱》;20031130;第21卷(第6期);第610页标题、摘要,第610页右栏倒数第1段 * |
| 疏水相互作用与亲水相互作用组合色谱纯化缩宫素;张晓鸥 等;《过程工程学报》;20100630;第10卷(第3期);598-602 * |
| 贺湘凌 等.利用β-环糊精键合固定相分离纯化葛根素.《色谱》.2003,第21卷(第6期),610-613. * |
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Effective date of registration: 20160519 Address after: Xiaoshan Port District Guali town melon 311241 Hangzhou Road, Zhejiang, No. 160 Patentee after: Zhejiang Hua Jun Pharmaceutical Co., Ltd. Patentee after: Institute of Process Engineering, Chinese Academy of Sciences Address before: 310012 No. 317, Wan Tong Road, Hangzhou, Zhejiang, Xihu District Patentee before: Hangzhou Huajin Pharmaceutical Co., Ltd. Patentee before: Institute of Process Engineering, Chinese Academy of Sciences |