CN102242135B - Rice serine hydroxymethyl transferase protein and function of coding gene thereof - Google Patents
Rice serine hydroxymethyl transferase protein and function of coding gene thereof Download PDFInfo
- Publication number
- CN102242135B CN102242135B CN 201110122676 CN201110122676A CN102242135B CN 102242135 B CN102242135 B CN 102242135B CN 201110122676 CN201110122676 CN 201110122676 CN 201110122676 A CN201110122676 A CN 201110122676A CN 102242135 B CN102242135 B CN 102242135B
- Authority
- CN
- China
- Prior art keywords
- gene
- plant
- mutant
- rice
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a rice serine hydroxymethyl transferase protein and a function of a coding gene thereof, wherein the protein has an amino acid sequence shown in SEQ ID NO:2, and the gene for coding the protein has a nucleotide sequence shown in SEQ ID NO:1. The invention also discloses a plasmid containing the gene, a plant expression vector and a host cell. The invention also discloses a lethal method for the seedling stage of cultivars. The plant expression vector is used for converting plant cells, and the converted plant cells are cultured into plants. In the invention, the site of a new gene cloned in a mottle leaf lethal mutant in the seedling stage can be mutated to obtain a seedling-stage lethal phenotype. The invention has broad application prospects in hybrid seed production and purification as well as seed intellectual property protection.
Description
Technical field
The invention belongs to plant genetic engineering field.The function that particularly relates to rice serine hydroxymethyl transferase protein and encoding gene thereof.Specifically, the present invention relates to a kind ofly utilize map-based cloning cloning rice genes encoding serine hydroxymethylase 1 (OsSHM1) gene, and utilize transgenic experiments to identify the function of this gene.
Background technology
Serine hydroxymethylase (Serine Hydroxymethyltransferase, SHMT) is that a kind of 5 ' pyridoxal phosphate (PLP) is the pyridoxal enzyme of coenzyme, and it plays very important effect in the one carbon metabolism of higher plant and photorespiration.There is the SHMT of two types in eukaryote, be kytoplasm serine hydroxymethylase (Cytosolic Serine Hydroxymethyltransferase, cSHMT) and plastosome serine hydroxymethylase (Mitochondrial Serine Hydroxymethyltran sferase, mSHMT).And only has cSHMT in prokaryotic organism.The major function of SHMT in plant is to provide Gly in the biosynthesizing of protein and purine, and provides 5,10-MTHF for the C1 storehouse.If plant lacks SHMT, usually can cause serious delayed growth.
Found the gene of 7 coding SHMT in Arabidopis thaliana, compared with prokaryotic organism, differentiation has occured in the SHMT function in plant.Studies show that the expression of only having SHMT1 is subject to inducing of light, so probably only have SHMT1 to bring into play its physiological function in the photorespiration metabolism.Mutant shmt1-1 wherein, its SHMT activity only has 15% of wild-type, the glycine decarboxylation can not be discharged CO
2, can not change glycine into Serine, photorespiration condition lower blade glycine accumulates in a large number, and content is more than 40 times of wild-type.Under adverse environmental factor, the shmt1 mutant shows as adverse circumstance responsive, and plant is sallow, blade necrosis etc.
At present, the functional study of plant serine hydroxymethylase gene is also very goed deep into, especially the effect of photorespiration also had a lot of arguements.In paddy rice, yet there are no the relevant report of serine hydroxymethylase and gene thereof, due to the mutant that lacks the rice serine hydroxymethyl transferase gene, be difficult to this gene is carried out deep functional study.The inventor has found the critical sites of the homologous protein OsSHM1 of SHM1 in the paddy rice by long-term and arduous research, obtains Herba Cissi Javanae lethal mutation in seedling stage body by sudden change OsSHM1 critical sites, has broad application prospects.
Summary of the invention
The invention provides a kind of rice seedling Herba Cissi Javanae lethal gene OsSHM1, this gene has the nucleotide sequence shown in SEQ IDNO:1.
Preferably, can add, replace in the nucleotide sequence shown in above-mentioned SEQ ID NO:1, insert or lack one or more Nucleotide and form the SAL1 derivative, this OsSHM1 derivative has identical function with rice seedling Herba Cissi Javanae lethal gene OsSHM1.Preferably, " C " of the 1300th of SEQ ID NO:1 the sports " T ".
The present invention also provides the protein of a kind of rice seedling Herba Cissi Javanae lethal gene coding, nucleotide sequence coded by shown in SEQ ID NO:1 of this protein, and it has the aminoacid sequence shown in SEQ ID NO.2.
Preferably, can add, replace, insert or delete the homologous sequence of one or more amino acid or other species in the aminoacid sequence of above-mentioned SEQ ID NO.2, thereby generate the derivative of aminoacid sequence.The Serine generation missense mutation of preferred the 434th, more preferably, the Serine missense mutation is leucine.
The present invention also provides a kind of method of the lethal mutation in seedling stage that cultivates plants, the method comprises with the nucleotide sequence transformed plant cells shown in SEQ ID NO:1, again the vegetable cell that transforms is cultivated into plant, wherein vegetable cell is preferably paddy rice, barley, wheat, corn, Chinese sorghum or sugarcane cell, especially is preferably rice cell.
The present invention also provides a kind of method of cultivating the rice seedling lethal mutation, the method comprises with the Plasmid Transformation rice cell that contains the nucleotide sequence shown in SEQ ID NO:1, again the cell culture that transforms is become plant, wherein plasmid contains said gene sequence SEQ ID NO:1.
The present invention also provides a kind of method of cultivating the rice seedling lethal mutation, the method comprises with the expression vector rice transformation cell that contains the nucleotide sequence shown in SEQ ID NO:1, again the cell culture that transforms is become plant, wherein expression vector contains said gene sequence SEQ ID NO:1.
The present invention also provides a kind of method of cultivating the rice seedling lethal mutation, the method comprises with the expression vector transformed plant cells that contains the nucleotide sequence shown in SEQ ID NO:1, again the cell culture that transforms is become plant, wherein expression vector contains said gene sequence SEQ ID NO:1.Wherein vegetable cell is preferably paddy rice, barley, wheat, corn, Chinese sorghum or sugarcane cell.
The present invention also provides a kind of host cell, and this host cell contains gene order SEQ ID NO:1 or derivatives thereof.Wherein host cell can be Bacillus coli cells, agrobatcerium cell or vegetable cell.
Rice seedling lethal gene OsSHM1 of the present invention (SEQ ID NO:1) separates from rice seedling Herba Cissi Javanae mutant lzs (lethal zebra seedling), and determines to control the mutant character of mutant by genetic analysis.
Above-mentioned new gene of cloning from Herba Cissi Javanae lethal mutation in seedling stage body of the present invention, this site is suddenlyd change can obtain lethal phenotype in seedling stage, has broad application prospects in hybrid seeding purification and seed intellectual property protection.
For the ease of understanding, below will the present invention be described in detail by concrete drawings and Examples.
Description of drawings
Fig. 1 is the phenotype analytical of lzs mutant.The plant on the left side is wild-type, and the plant on the right side is the lzs mutant;
Fig. 2 is the Primary Location of LZS gene on paddy rice the 3rd karyomit(e)
Fig. 3 is the Fine Mapping of LZS gene on paddy rice the 3rd karyomit(e)
Fig. 4 is the physical map of carrier pCAMBIA301-LZS
Fig. 5 is CAPS indicia designs schematic diagram
Fig. 6 is transfer-gen plant Molecular Detection figure, and WT is wild-type, and MT is the homozygous mutation body
Fig. 7 is the transgenic positive homozygous plants, and the left side is the non-transgenic contrast, and the right is transformed plant
Fig. 8 is the Photosynthetic Pigment content of lzs mutant
Fig. 9 is the photosynthesis of lzs mutant
Figure 10 is that the enzyme of lzs mutant is lived.POD is peroxidase, and SOD is superoxide-dismutase, and CAT is the hydrogen oxide enzyme, and MAD is mda.
Embodiment
In order to understand the present invention, the below further illustrates the present invention with embodiment, but does not limit the present invention.In following embodiment, method therefor if no special instructions, is the method that molecular biology commonly used, tissue culture technique and agronomy handbook are put down in writing.
The map based cloning of embodiment 1, LZS gene
1, the separation of paddy rice lzs mutant and genetic analysis
Mutant therefrom spends 11 groups of trainings to find in offsprings, and from two leaf stage, young leaves turns white, to blade in tri-leaf period withered (accompanying drawing 1) gradually.In T1 generation,, wild-type phenotype plant separates than being 35: 13 with the mutant plant, (the χ that meets 3: 1
2 c=0.03＜χ
2 0.05=3.84) separation relation, T1 is for seed for the individual plant results, choose at random 10 single-strain planting T2 generations, T2 is for 3 strain plant performance wild-type phenotypes in strain, no longer separate, separation has occured in the plant phenotype of 7 strains, and the segregation ratio of wild-type phenotype plant and mutant plant meets the separation relation of 3: 1, points out this mutant controlled by a pair of recessive nuclear gene.
2, rice Os SHM1 gene cloning:
(1) target group builds
Because mutant lzs is lethal seedling stage, the seed that can not obtain to isozygoty and long-grained nonglutinous rice parent are hybridized acquisition F
2Segregating population is used for the assignment of genes gene mapping, can only utilize heterozygote and long-grained nonglutinous rice parent to hybridize and obtain F
2For target group.Namely at T
1In segregating population, choose at random 6 strains and list from the plant of survival, obtain F with rice variety Long Tefu B hybridization respectively
0Seed, individual plant is gathered in the crops the selfed seed of listing and is planted T simultaneously
2In generation, is to identify T
1The genotype of individual plant.If T
2Do not occur separating for strain, show T
1Genotype for individual plant is homozygous wildtype, discards the F of this individual plant and Long Tefu B hybridization
0Seed; If the T of selfed seed plantation
2Occur separating for strain, show this T
1Genotype for individual plant is heterozygote.Plant the F of this individual plant and Long Tefu B hybridization
0Seed obtains the F1 plant, individual plant results F
1Plant seed is planted to get F
2In generation, approximately the phenotype separation appears in 1/2 strain, and selection is wherein that the individual plant of lzs phenotype extracts DNA for the assignment of genes gene mapping.
(2) gene mapping and cloning
Choose 120 and be evenly distributed on 12 karyomit(e)s of paddy rice have polymorphism between two parents SSR mark, the green seedling DNA pond of building up with the BSA method and mutant DNA pond (each 10 strain DNA balanced mix), utilize above-mentioned 120 to have polymorphic SSR mark to carry out polymorphism analysis to two ponds, being positioned at the long-armed SSR mark RM8277 (127.7cM) of the 3rd karyomit(e), RM6090 (144.5cM) and RM570 has strong skewness amplification.utilize subsequently RM8277 and RM6090 and near SSR mark RM5813 thereof, RM8269, RM3867 increases to 93 individual plants wherein, find LZS and above-mentioned mark close linkage, LZS is positioned at and RM8277 (127.7cM) and RM5813 (139.8) between, utilize subsequently the SSR sequence of announcing to choose 10 pairs of SSR primers between RM8277 (127.7cM) and RM5813 (139.8), wherein 5 pairs have polymorphism between two parents, utilize these 5 pairs of primers further above-mentioned 93 individual plants to be increased, further LZS is positioned between RM15857 and RM15887, and the genetic distance between two marks is respectively at a distance of 1.1cM and 0.5cM (accompanying drawing 2)
In order further to build and target gene linkage map more closely, extract F
2In colony, the DNA of whole 645 plant mutant body seedling carries out further Fine Mapping, utilizes whole 645 individual plants of RM15857 and RM15887 screening, and wherein RM15857 has 21 single crosses to change individual plant, and RM15887 has 8 single crosses to change individual plant.and long-grained nonglutinous rice 9311 sequence alignment analytical resultss fine according to the japonica rice Japan that announces, 16 STS marks have been developed between RM15857 and RM15887 mark, utilize these marks to spend in the parent 11 and Long Tefu B carry out variance analysis, have 14 and can amplify the wall scroll band, wherein 8 show polymorphism between the parent, utilize these 8 pairs of marks that the exchange individual plant that RM15857 and RM15887 filter out is increased, OsSHM1 further is positioned between RH3-17 and RH3-21, with RH3-20 be divided into from, two marks of RH3-17 and RH3-21 are positioned on two overlapped BAC, physical distance is about 49kb (accompanying drawing 3a).
Show the primer of polymorphism between the parent:
Prediction has 7 ORF in the 49kb interval, and 2 retrotransponsonses are wherein arranged, and all the other 5 ORF have all been carried out sequencing analysis, and finding has 1 base that sudden change has occured in ORF2, causes this to go out amino acid variation has occured.ORF2 genes encoding encoding serine hydroxymethyl transferases 1 (SHM1), the OsSHM1 gene contains 15 exons, 14 introns, missense mutation occurs in the 14th exon place, the T of the 1300th is replaced by C, and corresponding amino acid is leucine (accompanying drawing 3b) by the Serine missense mutation.This coding mutation has produced a new restriction enzyme site AflII, utilizes Afl II to design a CAPS mark, in order to identify the genotype of plant, is used for simultaneously identifying the complementary positive plant that isozygotys.
Embodiment 2:OsSHM1 function complementation experiment
1, complementary vector construction
The fragment that has complementary functions is chosen candidate gene LZS and is contained after the 5 ' UTR of whole ORF and gene translation codon ATG precontract 2.1kb and termination codon TAA approximately 3 ' the UTR of the 660bp about DNA fragmentation of 6.8kb altogether, is used for complementary Vector construction.Amplimer is:
5’-AGCAC
GTCGACCAAAAGCAGGCTTACTGCCACAAAG-3’(SEQ?IDNO：19)
3’-GTCCAC
AGATCTCATATGCCTCTTACTCTCACAGTTG-3’(SEQ?IDNO：20)
Amplified production contains Sal I and two restriction enzyme sites of Bgl II are used for being connected to the pCAMBIA1301 carrier.Pcr amplification amplification system (50 μ L): 2 * Primer STAR GC damping fluid, 25 μ L, dNTPs (2.5mmol/L) 8 μ L, each 1 μ L of primer (10mmol/L), TaKaRa Primer STAR Taq (5U/ μ L) 1 μ L, DNA (50ng/ μ L) 1.5 μ L, sterilized water 12.5 μ L.In the ABI 3700 enterprising performing PCR amplifications of PCR instrument.Reaction conditions is: 94 ℃ of denaturation 5min; 94 ℃, 30s, 68 ℃, 10min, 35 circulations; 72 ℃ are extended 10min.Reaction product detects with 1% agarose gel electrophoresis.Reclaim the purpose fragment, cut with Sal I and Bgl II enzyme and be connected on the pCAMBIA1301 carrier of cutting with same enzyme, 16 ℃ of water-baths connect spends the night, with the heat shock method, recombinant plasmid dna is transformed the bacillus coli DH 5 alpha competent cell, choose positive colony and shake bacterium extraction plasmid, adopt PCR and Sal I and Bgl II enzyme to cut dual evaluation recombinant plasmid, after determining to successfully construct complementary carrier, the evaluation of checking order is guaranteed to suddenly change without base.The complementary carrier pCAMBIA1301-LZS that successfully constructs is imported Agrobacterium (accompanying drawing 4) to be transformed.
2, the genotype identification of transformation receptor
Because mutant is lethal seedling stage, the mutant plant that can't obtain to isozygoty is collected seed and carries out genetic transformation for callus induction, therefore must select the seed with heterozygous plant to carry out genetic transformation.Produce new these characteristics of restriction enzyme site Afl II according to the mutational site, designed a CAPS mark
The CAPS primer:
P1：5’-TACGAACTTGTCTCTGGTGG-3’(SEQ?ID?NO：21)
P2：3’-AATCCCATTGCCCTTGTAG-3’(SEQ?ID?NO：22)
Enzyme is Afl II, P1 is positioned at the 5 ' end in mutational site, P2 is positioned at the 3 ' end in mutational site, be in simultaneously outside 3 ' end of complementary fragment, after carrying out PCR like this, the complementary fragment of the external source that transforms can't increase, the product of amplification only comprises the purpose fragment of genome itself, the PCR product is 1.6kb approximately, and after Afl II enzyme was cut, wild-type fragment was without restriction enzyme site, can not cut by enzyme, homozygous mutation body fragment enzyme is cut product and is contained 1.1kb and two fragments of 530bp, and heterozygote can produce 1.6kb, three fragments of 1.06kb and 530bp.Mutant is separated the offspring plant identify, the genotype of the phenotype of plant and CAPS Marker Identification fits like a glove, and can effectively identify the genotype of plant.Utilize this CAPS mark, be used for identifying the complementary positive plant that isozygotys (accompanying drawing 5).
3, genetic transformation
genetic transformation adopts agrobcterium-mediated transformation (Hiei etc., Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of T-DNA.Plant J, 1994, 6:271-282) utilize the callus of said mutation body maturation embryonal induction, after cultivating for 3 weeks through inducing culture, select the vigorous callus of growth as the acceptor that transforms, the EHA105 bacterial strain of pCAMBIA1301 empty carrier and pCAMBIA1301-LZS carrier is infected rice callus, in dark, after cultivating altogether 3 days under 25 ℃ of conditions, cultivate on the screening culture medium that contains 40mg/L Hygromycin.The screening kanamycin-resistant callus tissue was cultivated about 10 days containing on the pre-division culture medium of 50mg/L.The callus of breaking up is in advance gone on division culture medium cultivate under illumination condition.Obtain the resistant transgenic plant about one month.
4, the Molecular Identification of transfer-gen plant
At first the plant that utilizes this CAPS label screening to survive and, after being confirmed to be heterozygote, individual plant is gathered in the crops seed, evoked callus, transform pCAMBIA1301-LZS, the transformation seedlings that obtains is separated into 3 kinds of genotype, identify equally to only have and identify it is that the genotypic normal plant of homozygous mutation body is complementary successful plant with above-mentioned CAPS mark.The transformation seedlings of strain more than 150 that obtains is identified, screening 10 strain genetic backgrounds is complementary plant (accompanying drawing 6) of transgenosis of homozygous mutation body.This 10 strain transformed plant phenotype reverts to wild-type fully, shows and has complementary functions successfully.The gene that conclusive evidence is controlled the lethal phenotype of Herba Cissi Javanae in seedling stage is OsSHM1 (accompanying drawing 7).Clear and definite the DNA sequence dna of OsSHM1 gene (SEQ ID NO:1).
Example 3: Contents of Photosynthetic Pigments is measured
Get respectively mutant and come to spend the consistent fresh blade of 11 two leaf stage growing ways in lzs and wild-type, measure its chlorophyll content.Result shows the sudden change due to the leaf look, and the chlorophyll a of two leaf stage mutant lzs, the content of chlorophyll b and carotenoid are all significantly lower than wild-type, but chlorofucine hla/Chlb ratio no significant difference (accompanying drawing 8)
Example 4: the mensuration of chlorophyll fluorescence kinetic parameter
At two leaf stage, utilize portable gas exchange system Li-6400 (U.S. LI-COR company) and blue red light source leaf chamber thereof, measure the chlorophyll fluorescence kinetic parameter of spending 11 blades in mutant lzs and wild-type.Result shows the maximal photochemistry efficiency of lzs mutant PSII significantly lower than wild-type, the PSII reactive center light energy use efficiency of mutant is described and originally the luminous energy capture rate is lower; The observed value of the Relative electron transport rate of mutant and actual light chemical efficiency is 0, illustrates that mutant does not almost have photochemical activity (accompanying drawing 9).
Example 5: the mensuration of soluble protein content
The mensuration of soluble protein content adopts the Xylene Brilliant Cyanine G method.Measurement result shows that the soluble proteins of mutant obviously reduces, and is about 20% of wild-type.Various activities of antioxidant enzymes in mutant and mda content obviously raise, wherein the POD activity is about 1.2 times of wild-type, the SOD activity is about 4.4 times of wild-type, and the CAT activity is about 2 times of wild-type, and mda (MDA) is 6.8 times (accompanying drawings 10) of wild-type.
Claims (1)
1. the protein of rice seedling Herba Cissi Javanae lethal gene coding, it is the Serine generation missense mutation of the 434th of the aminoacid sequence shown in SEQ ID NO:2, sports leucine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110122676 CN102242135B (en) | 2011-05-11 | 2011-05-11 | Rice serine hydroxymethyl transferase protein and function of coding gene thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110122676 CN102242135B (en) | 2011-05-11 | 2011-05-11 | Rice serine hydroxymethyl transferase protein and function of coding gene thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102242135A CN102242135A (en) | 2011-11-16 |
| CN102242135B true CN102242135B (en) | 2013-11-06 |
Family
ID=44960382
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110122676 Expired - Fee Related CN102242135B (en) | 2011-05-11 | 2011-05-11 | Rice serine hydroxymethyl transferase protein and function of coding gene thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102242135B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103173424A (en) * | 2013-04-16 | 2013-06-26 | 中国农业科学院生物技术研究所 | RPRP (Rice Photorespiration Related Protein)-1 capable of increasing photosynthetic efficiency of plants as well as coding gene and application of RPRP-1 |
| CN104017819A (en) * | 2014-06-23 | 2014-09-03 | 中国科学院合肥物质科学研究院 | Efficient method for in-vitro evolution of serine hydroxymethyltransferase |
| CN109666682B (en) * | 2019-01-29 | 2022-03-04 | 南京农业大学 | Rice serine hydroxymethyl transferase coding gene OsSHM4 mutant and application thereof |
| CN116103265B (en) * | 2023-01-10 | 2024-05-14 | 中国科学院西北生态环境资源研究院 | Salicornia europaea phosphatase mutant protein with reduced epidermal hair and application thereof |
| CN116121298B (en) * | 2023-04-18 | 2023-06-16 | 河南大学三亚研究院 | Application of inhibiting expression of HSRP1 gene in improving heat resistance of plants |
-
2011
- 2011-05-11 CN CN 201110122676 patent/CN102242135B/en not_active Expired - Fee Related
Non-Patent Citations (5)
| Title |
|---|
| BUELL et al.GENBANK:ABF98772.1.《GENBANK》.2006,1. * |
| GENBANK:ABF98772.1;BUELL et al;《GENBANK》;20060607;1 * |
| Photorespiration-deficient Mutants of Arabidopsis thalianaLacking Mitochondrial Serine Transhydroxymethylase Activit1;C. R. SOMERVILLE et al;《Plant Physiol.》;19811231;第67卷;666-671 * |
| 植物丝氨酸羟甲基转移酶及其生理作用研究进展;马莉 等;《安徽农业科》;20081231;第36卷(第4期);1357- 1359, 1404 * |
| 郭玉朋.水稻(Oryza sativa L.)光呼吸突变体线粒体丝氨酸羟甲基.《中国博士学位论文全文数据库农业科技辑》.2010,(第10期),44-50. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102242135A (en) | 2011-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9663794B2 (en) | Heat-resistance rice gene OsZFP, screening marker and separation method thereof | |
| EP2846621B1 (en) | Corn event mon 87411 | |
| CN113201531B (en) | Transgenic corn event LW2-1 and detection method thereof | |
| CN102316720A (en) | Corn event 5307 | |
| BRPI0908267B1 (en) | Production method of a drought tolerant maize plant | |
| CN111206031A (en) | Nucleic acid sequence for detecting corn plant NAZ-4 and detection method thereof | |
| CN103290027A (en) | Protein for regulating and controlling chloroplast growth and gene and application thereof | |
| CN102242135B (en) | Rice serine hydroxymethyl transferase protein and function of coding gene thereof | |
| CN109971880B (en) | Nucleic acid sequence for detecting corn plant DBN9508 and detection method thereof | |
| CN102766618B (en) | Rice OsICL protein and coding gene thereof, and application of the two | |
| CN102766697B (en) | Molecular marking method for detecting imidazolone herbicide resisting gene of cabbage type rape | |
| CN102212122A (en) | Mutant lethal gene for controlling development of rice chloroplasts and application thereof | |
| CN103172715B (en) | Plant epidermal hair controlling gene and application thereof | |
| CN110881367A (en) | Corn event Ttrans-4 and methods of use thereof | |
| WO2015007241A1 (en) | Molecular marker | |
| CN105073994A (en) | Brassica plants comprising mutant DA1 alleles | |
| CN100569949C (en) | Rice chlorophyll synthase mutant gene and genetically engineered thereof are used | |
| CN116287384B (en) | Nucleic acid molecule of insect-resistant herbicide-resistant corn transformation event LD05, detection method and application thereof | |
| CN111154767B (en) | Root length regulatory gene LOGL5, corresponding construct and application thereof | |
| CN114015700B (en) | Application of soybean gene GmFER1 in salt stress resistance of plants | |
| CN105294846A (en) | Application of CIC1 protein in regulation and control of plant low-temperature resistance | |
| CN112457385B (en) | Application of gene LJP1 for controlling rice growth period | |
| CN113278721B (en) | Transgenic corn event LW2-2 and detection method thereof | |
| CN104650204B (en) | The albumen related to rice ATP transports and Development of Chloroplasts and its encoding gene and application | |
| CN109609515B (en) | Gene for regulating growth and development of chloroplast and influencing leaf color under low-temperature stressCDE4And applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131106 |