CN102241771A - Duck egg yolk antibody for preventing and treating virus hepatitis of duckling, and preparation method thereof - Google Patents
Duck egg yolk antibody for preventing and treating virus hepatitis of duckling, and preparation method thereof Download PDFInfo
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- CN102241771A CN102241771A CN2011101300866A CN201110130086A CN102241771A CN 102241771 A CN102241771 A CN 102241771A CN 2011101300866 A CN2011101300866 A CN 2011101300866A CN 201110130086 A CN201110130086 A CN 201110130086A CN 102241771 A CN102241771 A CN 102241771A
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Abstract
The invention discloses a duck egg yolk antibody for preventing and treating virus hepatitis of ducklings, and a preparation method thereof. The duck egg yolk antibody is extracted from the primiparous eggs of a high-immunity homebred breed duck starting laying for one month. The preparation method comprises the following steps of: (a) highly immunizing the breed duck before laying; (b) collecting high-immunity primiparous breed duck eggs; (c) cleaning and sterilizing; (d) separating egg yolks; (e) diluting; (f) stirring and filtering; and (g) packaging and preserving. After the egg yolks are continuously diluted for 2 times by using normal saline, the DHV (Duck Hepatitis Virus) precipitating antibody is detected by a conventional method, the antibody titer is more than 1:64, and the HI titer of H5 is more than 1:512. 0.5mL of the duck egg yolk antibody is subcutaneously injected to a 3-day-old duckling for preventing virus hepatitis; and 1mL of egg yolk antibody to which antibiotics are added is subcutaneously injected to a duck for treating virus hepatitis, and the egg yolk antibody is re-injected to a seriously diseased duck every other day.
Description
Technical field
The present invention relates to a kind of yolk antibody, relate in particular to a kind of duck yolk antibody of preventing and treating the duckling viral hepatitis; Also relate to a kind of method for preparing the duck yolk antibody of described control duckling viral hepatitis.
Background technology
Duck viral hepatitis is the duckling virus disease of normal generation, and high immunity yolk antibody is the most certain method for the treatment of the duckling viral hepatitis at present.The yolk antibody of present clinical use, all being commercial production unit extracts from general height is exempted from the egg of laying ducks, different local ducklings exist following risk when using this yolk antibody, use dangerous: xenogenesis duck group genetic background is unclear, perhaps carry bad gene or potential vertical transmission cause of disease, as avian leukosis etc.; Height is exempted from the laying ducks antibody horizontal understand not quite clearly, perhaps exist strain incompatible.In addition, if the laying ducks oviposition period is long, the yolk antibody level can descend, and performance and effect can reduce.And common laying ducks is laid eggs individual big, the market price height, but be used to prepare yolk antibody, and it is low to tire, and economic worth is not high.
Summary of the invention
The object of the present invention is to provide a kind of duck yolk antibody of preventing and treating the duckling viral hepatitis, this duck yolk antibody antibody horizontal height, safe in utilization, the economic worth height; A kind of method for preparing the duck yolk antibody of described control duckling viral hepatitis also is provided.
In order to solve the problems of the technologies described above, the duck yolk antibody of control duckling viral hepatitis of the present invention is to open from the kind duck through height is exempted from that oneself raises to extract gained first the laying eggs of producing in 1 month.
The method of the duck yolk antibody of the described control of preparation of the present invention duckling viral hepatitis comprises the steps:
A. planting duck opens antenatal height and exempts from: kind of a duck group is opened antenatal carry out bird flu, duck plague and the immunity of duck viral hepatitis high strength;
B. gather height and exempt from primiparity kind duck's egg: gather through the kind duck group that height is exempted from and open first the laying eggs of producing in 1 month;
C. cleaning-sterilizing: the height that will gather is exempted from primiparity kind duck's egg cleaning back with medicining liquid dipping 3-5 minute, dries;
D. separate yolk: open eggshell at sterilisable chamber, pour out egg content, isolate yolk;
E. dilution: in sterilisable chamber, the ratio (volume ratio) that adds water according to yolk and institute is the ratio of 3.5: 6.5 or 1: 2, and adding contains the physiological saline of gentamicin and analytical pure formaldehyde on yolk;
F. agitation and filtration: under aseptic condition, yolk diluent is stirred into suspension, then through 50 order membrane filtrations;
G. encapsulation is preserved: under aseptic condition, filtrate is sub-packed in disinfectant vial or disposable plastic bottle, covers plug, seal up aluminium lid, and labelled, to preserve stand-byly, storage temperature is 4-8 ℃ or-20 ℃.
Described physiological saline contains 0.85%NaCl, and through 20 minutes autoclavings of 1kg/ ㎝ 2 pressure or boil sterilization in 20 minutes, adding gentamicin, to make dilution back gentamicin final concentration be 100 IU/mL, and adding formaldehyde again, to make its final concentration be 0.08%.
Stick the source place label for the made yolk antibody of the kind duck's egg of different areas, use for this area duckling.
The yolk antibody outward appearance that makes is that fresh yolk antibody is yellow, is even matter suspension, and finished product has precipitated and separated slightly after placing for some time under the 4-8 ℃ of condition, do not tire but do not influence, and the time spent shakes up and gets final product; Present the suspension shape after-20 ℃ of ice-melts of preserving of freezing.
Because the duck yolk antibody of control duckling viral hepatitis of the present invention is to extract gained from the kind duck through height is exempted from that oneself raises is just laid eggs, oneself primiparity kind duck's egg height is exempted from antigen and is come from the immunization that this area needs, antibody with strong points, prevention effect is certain, and provenance is clear, do not exist and introduce the former risk of vertical transmission venereal disease, overcome the deficiency of using other commercial yolk antibodies, safe in utilization; Open that antenatal it is higher to open the yolk antibody level of producing the primiparity kind duck's egg in 1 month through multiple spot immunization repeatedly owing to plant the duck group, the height of tiring, performance and prevention effect are also better.And the yolk antibody level is higher, and it is little to tire, and the market price is low, is used to prepare yolk antibody, and the height of but tiring has improved the economic worth of primiparity kind duck's egg.
Titration: conventional method detected the duck viral hepatitis precipitating antibody after test sample was done continuous 2 times of dilutions with physiological saline, and antibody titers is more than 1: 64.Bird flu hemagglutination inhibition antibody (HI) titration: test sample is made HI that conventional method after continuous 2 times of dilutions detects H5 with physiological saline and is tired and be about more than 1: 512.
Using method: when 1, being used to prevent, duckling subcutaneous injection in 3 day age 0.5mL/ only; When 2, being used for the treatment of, can add microbiotic in yolk antibody, subcutaneous injection 1mL/ only injects once the next day of serious sufferer duck again.
The clinical result of use (2006 annual data) of duck yolk antibody control duckling viral hepatitis of the present invention
Embodiment
Prepare the duck yolk antibody of control duckling viral hepatitis of the present invention, preparation work has strict demand:
1, preparation physiological saline: contain the 0.85%NaCl pure water, through 20 minutes autoclavings of 1kg/ ㎝ 2 pressure or boil sterilization in 20 minutes, cool standby, add gentamicin make dilution afterwards the gentamicin final concentration be 100 IU/mL, adding formaldehyde, to make its final concentration be 0.08%.
2, prepare finished product yolk antibody bottle: after cleaning up, encase bottleneck with kraft paper, 150 ℃ of dry heat sterilization 1h, standby, or use the disposable plastic bottle.
3, prepare plug, 50 order filtering membranes, gauze etc., standby through 15 pounds of 20 minutes autoclaving after drying.
4, boil before other vessel productions or steam disinfection 20 minutes.
5, production is sterilized 30 minute or more with ultraviolet lamp with sterilisable chamber before production.
Whole process of preparation all is to carry out under aseptic condition.
Stick the source place label for the made yolk antibody of the kind duck's egg of different areas, use for this area duckling.
Claims (3)
1.
A kind of duck yolk antibody of preventing and treating the duckling viral hepatitis is characterized in that: described yolk antibody is to extract gained from the kind duck through height is exempted from that oneself raises is just laid eggs.
2.
The preparation method of the described duck yolk antibody of a kind of claim 1 is characterized in that comprising the steps:
A. planting duck opens antenatal height and exempts from: kind of a duck group is opened antenatal carry out bird flu, duck plague and the immunity of duck viral hepatitis high strength;
B. gather height and exempt from primiparity kind duck's egg: gather through the kind duck group that height is exempted from and open first the laying eggs of producing in 1 month;
C. cleaning-sterilizing: the height that will gather is exempted from primiparity kind duck's egg cleaning back with medicining liquid dipping 3-5 minute, dries;
D. separate yolk: open eggshell at sterilisable chamber, pour out egg content, isolate yolk;
E. dilution: in sterilisable chamber, the ratio (volume ratio) that adds water according to yolk and institute is the ratio of 3.5: 6.5 or 1: 2, and adding contains the physiological saline of gentamicin and analytical pure formaldehyde on yolk;
F. agitation and filtration: under aseptic condition, yolk diluent is stirred into suspension, then through 50 order membrane filtrations;
G. encapsulation is preserved: under aseptic condition, filtrate is sub-packed in disinfectant vial or disposable plastic bottle, covers plug, seal up aluminium lid, and labelled, to preserve stand-byly, storage temperature is 4-8 ℃ or-20 ℃.
3.
Preparation method according to claim 2 is characterized in that: described physiological saline contains 0.85%NaCl, warp 
20 minutes autoclavings of pressure or boil sterilization in 20 minutes, adding gentamicin, to make dilution back gentamicin final concentration be 100 IU/mL, adding formaldehyde again, to make its final concentration be 0.08%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201110130086.6A CN102241771B (en) | 2011-05-19 | 2011-05-19 | Duck egg yolk antibody for preventing and treating virus hepatitis of duckling, and preparation method thereof |
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| CN201110130086.6A CN102241771B (en) | 2011-05-19 | 2011-05-19 | Duck egg yolk antibody for preventing and treating virus hepatitis of duckling, and preparation method thereof |
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| CN102241771B CN102241771B (en) | 2014-02-19 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716484A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Duck virus hepatitis yolk antibody freeze-dried powder and preparation method thereof |
| CN102772799A (en) * | 2012-05-31 | 2012-11-14 | 郑州后羿制药有限公司 | Duplex egg yolk antibody freeze-drying powder for duck virus hepatitis and duck plague and preparation method thereof |
| CN102793923A (en) * | 2012-08-29 | 2012-11-28 | 广州格雷特生物科技有限公司 | Duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof |
| CN103709247A (en) * | 2013-12-30 | 2014-04-09 | 哈药集团生物疫苗有限公司 | Divalent egg yolk antibodies of type I and novel duck hepatitis as well as preparation method and application thereof |
| CN103992403A (en) * | 2014-05-23 | 2014-08-20 | 安徽科技学院 | Method for removing lipoprotein and lipid in goose/duck yolk by using freezing method |
| CN104162161A (en) * | 2013-07-24 | 2014-11-26 | 河南联合英伟饲料有限公司 | Composition for resisting duck viral hepatitis, freeze-dried powder, and preparation method and use of freeze-dried powder |
| CN106749639A (en) * | 2016-11-23 | 2017-05-31 | 临沂大学 | A kind of new H5 subtype avian influenzas Yolk antibody replaces the method for serum antibody monitoring |
| CN107982532A (en) * | 2017-12-05 | 2018-05-04 | 安徽强英鸭业集团有限公司 | A kind of duck hepatitis A virus antigen-antibody complex vaccine and preparation method thereof |
| CN112552398A (en) * | 2020-11-18 | 2021-03-26 | 辽宁益康生物股份有限公司 | Duck viral hepatitis egg yolk antibody and preparation method thereof |
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| CN101475639A (en) * | 2008-08-19 | 2009-07-08 | 福州大北农生物技术有限公司 | Mixed yolk antibody for preventing and treating duck infectious serositis and preparation thereof |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716484A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Duck virus hepatitis yolk antibody freeze-dried powder and preparation method thereof |
| CN102772799A (en) * | 2012-05-31 | 2012-11-14 | 郑州后羿制药有限公司 | Duplex egg yolk antibody freeze-drying powder for duck virus hepatitis and duck plague and preparation method thereof |
| CN102793923A (en) * | 2012-08-29 | 2012-11-28 | 广州格雷特生物科技有限公司 | Duck tembusu virus (DTMUV) disease immunotherapy preparation and preparation method thereof |
| CN102793923B (en) * | 2012-08-29 | 2016-08-03 | 广州格雷特生物科技有限公司 | A kind of duck tembusu virus disease immunity therapeutic preparation and preparation method thereof |
| CN104162161B (en) * | 2013-07-24 | 2016-09-21 | 河南联合英伟饲料有限公司 | A kind of anti-duck viral hepatitis compositions, lyophilized powder, preparation method and application |
| CN104162161A (en) * | 2013-07-24 | 2014-11-26 | 河南联合英伟饲料有限公司 | Composition for resisting duck viral hepatitis, freeze-dried powder, and preparation method and use of freeze-dried powder |
| CN103709247B (en) * | 2013-12-30 | 2016-02-10 | 哈药集团生物疫苗有限公司 | Preparation method of a kind of I type, Novel duck hepatitis divalence yolk antibody and products thereof and application |
| CN103709247A (en) * | 2013-12-30 | 2014-04-09 | 哈药集团生物疫苗有限公司 | Divalent egg yolk antibodies of type I and novel duck hepatitis as well as preparation method and application thereof |
| CN103992403A (en) * | 2014-05-23 | 2014-08-20 | 安徽科技学院 | Method for removing lipoprotein and lipid in goose/duck yolk by using freezing method |
| CN106749639A (en) * | 2016-11-23 | 2017-05-31 | 临沂大学 | A kind of new H5 subtype avian influenzas Yolk antibody replaces the method for serum antibody monitoring |
| CN107982532A (en) * | 2017-12-05 | 2018-05-04 | 安徽强英鸭业集团有限公司 | A kind of duck hepatitis A virus antigen-antibody complex vaccine and preparation method thereof |
| CN107982532B (en) * | 2017-12-05 | 2021-02-12 | 安徽强英鸭业集团有限公司 | Duck hepatitis A virus antigen-antibody complex vaccine and preparation method thereof |
| CN112552398A (en) * | 2020-11-18 | 2021-03-26 | 辽宁益康生物股份有限公司 | Duck viral hepatitis egg yolk antibody and preparation method thereof |
| CN112552398B (en) * | 2020-11-18 | 2021-08-17 | 辽宁益康生物股份有限公司 | Duck viral hepatitis egg yolk antibody and preparation method thereof |
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| CN102241771B (en) | 2014-02-19 |
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Denomination of invention: A duck yolk antibody for prevention and treatment of duck viral hepatitis and its preparation method Effective date of registration: 20201229 Granted publication date: 20140219 Pledgee: Industrial and Commercial Bank of China Limited Linwu sub branch Pledgor: Hunan Linwu Shunhua Duck Development Co.,Ltd. Registration number: Y2020430000055 |
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