CN102240314A - Medicine for treating urinary tract infection - Google Patents
Medicine for treating urinary tract infection Download PDFInfo
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- CN102240314A CN102240314A CN2011100722112A CN201110072211A CN102240314A CN 102240314 A CN102240314 A CN 102240314A CN 2011100722112 A CN2011100722112 A CN 2011100722112A CN 201110072211 A CN201110072211 A CN 201110072211A CN 102240314 A CN102240314 A CN 102240314A
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Abstract
The invention belongs to the technical field of medicaments, and in particular relates to a medicine for treating urinary tract infection. The main active component of the medicine disclosed by the invention is polysaccharide extracted from caryophyllaceae vaccaria segetalis (Neck.) Garcke. The polysaccharide in the invention is from vaccaria segetalis (Neck.) Garcke seeds. A method for preparing the vaccaria segetalis (Neck.) Garcke polysaccharide and vaccaria segetalis (Neck.) Garcke polysaccharide medicine composition provided by the invention is simple and effective, and capable of being prepared into a variety of common oral forms, such as solid, liquid and the like. The medicine disclosed by the invention is mainly used for treating the urinary tract infection as well as frequent micturition, urgent urination and odynuria caused by diabetes. Compared with the traditional medicines, the medicine disclosed by the invention has an obvious curative effect, low recurrence rate and no toxic and side effects, and the product quality is stable and controllable.
Description
One. technical field
The present invention relates to a kind of medicine that is used for the treatment of urinary tract infection and preparation method thereof, belong to medical technical field.
Two. background technology
Urinary tract infection is meant a large amount of microbial reproductions in the urinary tract and the urinary tract inflammation that causes, sees with female patient more.Urinary tract infection is clinical in one of common bacterial infectious disease, is only second to respiratory tract infection in infectious disease.Common pathogen is the G-bacillus, accounts for 80-90%, and is wherein common with escherichia coli, secondly is Bacillus proteus, klebsiella bacillus, bacillus pyocyaneus etc.; The G+ coccus infects the 5-10% that accounts for the urine sense, mainly is streptococcus faecalis, staphylococcus.Also have special infection such as mycobacterium tuberculosis infection in addition.The clinical characters of urinary tract infection mainly is frequent micturition, urgent micturition, dysurea.Present Therapeutic Method comprises general treatment and Drug therapy.General treatment refers to and will lie up, polydipsia water, and the sodium bicarbonate alkalized urine alleviates irritation sign of bladder, strengthens the antibiotics curative effect.Most of western medicine, the i.e. antibiotic therapy of adopting of Drug therapy.Common antibiotics comprises sulfonamides (bactrim etc.), beta-lactam (penicillins, cephalosporins), aminoglycosides (gentamycin, amikacin etc.), quinolones (pipemidic acid, ofloxacin, ciprofloxacin etc.).But because antibiotic antimicrobial spectrum is limited, and there is drug resistance in antibacterial, the life-time service antibiotic, and not only curative effect reduces, even also some untoward reaction can occur.In view of this problem, domesticly developed multiple Chinese patent medicine kind successively, as SANJIN PIAN, NIAOGANNING CHONGJI, lead red ball etc.But these Chinese medicine produce effects are slow, and treatment cycle is longer, to acute urinary tract infection curative effect deficiency.In addition, above-mentioned Chinese traditional patent formulation preparation material base complexity, effective ingredient is indeterminate, the quality controllability deficiency, thereby curative effect is stable inadequately.
Vaccaria segetalis (Vaccaria segetalis (Neck.) Garcke) is a Caryophyllaceae Vaccaria plant, has another name called Semen Vaccariae, milk rice, Fructus Hordei Vulgaris cattle, not femalely stays, king cow, SEMEN VACCARIAE.Shennong's Herbal is classified as top grade, but informal dress has no side effect.Vaccaria segetalis mainly is used as medicine with seed.The Chinese medicine name of Vaccaria segetalis seed is called Semen Vaccariae, has another name called to prohibit the palace flower, cuts JINHUA, Herba Clematidis Huchouensis grass, the silver-colored platform of gold small cup, wheat Lan Zi etc., tradition clinical be used for dysmenorrhes, amenorrhea more; Agalactia; Acute mastitis; The Vaccaria segetalis research and development that diseases such as inducing diuresis for treating stranguria syndrome are present remain in a lot of deficiencies: though the record Vaccaria segetalis has the drug effect of inducing diuresis for treating stranguria syndrome in 1 classic of TCM, its drug activity and active ingredient thereof there is no the pertinent literature report; 2, the research to Vaccaria segetalis mainly concentrates on small-molecule substance, does not see the Vaccaria segetalis macromolecular substances so far, comprises the structure and the active research report of polysaccharide; 3, traditionally, Vaccaria segetalis is only used in Chinese medicine compound, the report of the independent medication of Shang Weijian.
Vaccaria segetalis polysaccharide among the present invention and the extract that contains the Vaccaria segetalis polysaccharide can be made into common various oral formulations, are used for the treatment of urinary tract infection.Compare with existing urinary tract infection medicine, the characteristics that have taking convenience, curative effect is rapid significantly, composition and effectiveness is comparatively clear and definite, stable and controllable for quality, have no side effect, relapse rate are low are particularly useful for the treatment of acute urinary tract infection.
Three. summary of the invention
One of purpose of the present invention is to overcome the deficiency that prior art exists, and it is the medicine that contains the Vaccaria segetalis polysaccharide that a kind of main active is provided, and the source of Vaccaria segetalis polysaccharide is the seed of pinkwort Vaccaria segetalis (Vaccaria segetalis (Neck.) Garcke).
Another object of the present invention is to provide a kind of application of pharmaceutical preparation in the medicine of the frequent micturition for preparing the initiation of treatment urinary tract infection and diabetes, urgent micturition, dysurea that contains the Vaccaria segetalis polysaccharide.
Innovation part of the present invention is: discover first that 1, the Vaccaria segetalis polysaccharide is the important active ingredient of treatment urinary tract infection; 2, Vaccaria segetalis is all used the treatment urinary tract infection with the other drug compatibility traditionally, but this patent discovery Vaccaria segetalis is used for the treatment of the urinary tract infection drug effect separately significantly, fast; 3, Vaccaria segetalis polysaccharide treatment urinary tract infection drug effect is clear and definite, does not see the single Chinese patent medicine of other treatment urinary tract infection so far.In view of Vaccaria segetalis and the secular human history of extract thereof, its safety is extensively admitted, and this means that the present invention has bright potential applicability in clinical practice.
To achieve these goals, the invention provides a kind of Vaccaria segetalis polyoses extract: obtain by the extraction of Caryophyllaceae Vaccaria plant Vaccaria segetalis (Vaccaria segetalis (Neck.) Garcke) raw material; Described Vaccaria segetalis raw material is the Vaccaria segetalis seed.
Described Vaccaria segetalis seed is the Vaccaria segetalis seed after concocting.
Described Vaccaria segetalis polyoses extract is faint yellow, and total sugar content is 30-90wt%.
The present invention also provides a kind of extracting method of Vaccaria segetalis polyoses extract: gets the Vaccaria segetalis raw material, adds the distilled water of 5-15 times of weight, and heating and refluxing extraction 3-5 time or microwave extraction 2-3 time, merge extractive liquid, filters, and the filtrate heating concentrates; Adding ethanol to concentration of alcohol under stirring in concentrated extracting solution is 50-95%, leaves standstill, and collecting precipitation obtains the Vaccaria segetalis crude polysaccharides.
Also comprise in described Vaccaria segetalis crude polysaccharides and add dissolved in distilled water, add trichloroacetic acid or Sevag reagent protein precipitation, perhaps use proteasome degradation albumen, adopt ethanol acetone precipitation, ultrafiltration or dialysis to reclaim polysaccharide then, absolute ethanol washing 2-5 time is drying to obtain.
The present invention also provides a kind of purposes of Vaccaria segetalis polyoses extract, is used to prepare the purposes of urinary tract infection medicine, and indication is urgent micturition, frequent micturition or the dysurea that urinary tract infection or diabetes cause.
The present invention also provides a kind of pharmaceutical composition that is used for the treatment of urinary tract infection, it is characterized in that being made up of described Vaccaria segetalis polyoses extract and acceptable accessories.
The content of described Vaccaria segetalis polyoses extract is 1-99.9wt%, and adjuvant is 0.1-99wt%.
Fig. 1 is the infared spectrum of Vaccaria segetalis polysaccharide.Wherein Vaccaria segetalis polysaccharide sample preparation methods is embodiment 1 method, and infrared spectrum analysis adopts the KBr pressed disc method.Sample has typical polysaccharide feature.The absorption band of polysaccharide: at 3380cm
-1Strong and wide-OH absworption peak is arranged, at 2928cm
-1Near the moderate strength absworption peak be saturated C-H stretching vibration (for-CH ,-CH
2Absworption peak); 1154cm
-1, 1080cm
-1, 1023cm
-1The strong peak at place is the pyranose ring characteristic absorption peak, is the nonsymmetrical vibration peak of glycosidic bond C-O-C; Infrared spectrum is at 890cm
-1Near no absworption peak, and at 848cm
-1(844 ± 8) cm
-1There is absworption peak at the place, shows and contains α-pyranoside.At 875cm
-1And 810cm
-1The place does not have absorption, shows not contain mannopyranose glycosides, gala pyranoside.
The specific embodiment
The present invention further illustrates by the following example:
It is an amount of to get the Vaccaria segetalis seed, with 5 times of amount distilled water reflux, extracts three times, and each extraction time is 1.5 hours, merges three times extracting solution, and 150 order double-layer filter cloths filter, and the filtrate heating is concentrated into 1/3 of original volume; Stir and in concentrated extracting solution, to add 4 times of ethanol down, leave standstill 12h after, collecting precipitation obtains the Vaccaria segetalis crude polysaccharides.In the polysaccharide raw sediment, add dissolved in distilled water, make it abundant redissolution, add trichloroacetic acid to 1-10% concentration, leave standstill 3h after the stirring, the solution heating is concentrated into 1/3 of original volume, stirs to add 4 times of ethanol down in concentrated extracting solution, after leaving standstill 12h, collecting precipitation.Precipitation is with an amount of absolute ethanol washing 3 times, and the Vaccaria segetalis that obtains after the drying after the Deproteinization is made with extra care polysaccharide.The refining polysaccharide of this Vaccaria segetalis can further be prepared as various pharmaceutically acceptable compositionss and various dosage form.
Gained Vaccaria segetalis polysaccharide color is faint yellow, and total sugar content is 80%.
Embodiment 2
It is an amount of to get the Vaccaria segetalis seed, with 5 times of amount distilled water reflux, extracts three times, and each extraction time is 1.5 hours, merges three times extracting solution, and 150 order double-layer filter cloths filter, and the filtrate heating is concentrated into 1/3 of original volume; Stir and in concentrated extracting solution, to add 4 times of ethanol down, leave standstill 12h after, collecting precipitation obtains the Vaccaria segetalis crude polysaccharides.Add dissolved in distilled water in the polysaccharide raw sediment, make it abundant redissolution, regulating pH value is 7, adds 3%, 65 ℃ of warm macerating 1.5h of trypsin, adds 2.5%, 55 ℃ of warm macerating 1.5h of papain again.It with molecular cut off 10,000 filter membrane ultrafiltration.Collect trapped substance, add 500mL75% ethanol, leave standstill 12h after, collecting precipitation.Precipitation is with an amount of absolute ethanol washing 3 times, and the Vaccaria segetalis that obtains after the drying after the Deproteinization is made with extra care polysaccharide.The refining polysaccharide of this Vaccaria segetalis can further be prepared as various pharmaceutically acceptable compositionss and various dosage form.
Gained Vaccaria segetalis polysaccharide color is faint yellow, and total sugar content is 83%.
Embodiment 3
It is an amount of to get the Vaccaria segetalis seed, adds 5 times of amount distilled water, carries out microwave extraction, and microwave power is 600w, extracts twice, and each extraction time is 10min, merges 2 times extracting solution, and 150 order double-layer filter cloths filter, and the filtrate heating is concentrated into 1/3 of original volume; Stir and in concentrated extracting solution, to add 4 times of ethanol down, leave standstill 12h after, collecting precipitation obtains the Vaccaria segetalis crude polysaccharides.Add dissolved in distilled water in the polysaccharide raw sediment, be heated to 70, make it abundant redissolution, solution heating is concentrated into 1/3 of original volume, stirs to add 4 times of ethanol down in concentrated extracting solution, leave standstill 12h after, collecting precipitation.Precipitation obtains Vaccaria segetalis and makes with extra care polysaccharide with an amount of absolute ethanol washing 3 times after the drying.The refining polysaccharide of this Vaccaria segetalis can further be prepared as various pharmaceutically acceptable compositionss and various dosage form.
Gained Vaccaria segetalis polysaccharide color is faint yellow, and total sugar content is 75%.
Embodiment 4
It is an amount of to get the Vaccaria segetalis seed of frying after the process of preparing Chinese medicine, with 5 times of amount distilled water reflux, extracts three times, and each extraction time is 1.5 hours, merges three times extracting solution, and 150 order double-layer filter cloths filter, and filtrate heating is concentrated into 1/3 of original volume; Stir and in concentrated extracting solution, to add 4 times of ethanol down, leave standstill 12h after, collecting precipitation obtains the Vaccaria segetalis crude polysaccharides.In the polysaccharide raw sediment, add dissolved in distilled water, make it abundant redissolution, add trichloroacetic acid to 1-10% concentration, leave standstill 3h after the stirring, the solution heating is concentrated into 1/3 of original volume, stirs to add 4 times of ethanol down in concentrated extracting solution, after leaving standstill 12h, collecting precipitation.Precipitation is with an amount of absolute ethanol washing 3 times, and the Vaccaria segetalis that obtains after the drying after the Deproteinization is made with extra care polysaccharide.The refining polysaccharide of this Vaccaria segetalis can further be prepared as various pharmaceutically acceptable compositionss and various dosage form.
Gained Vaccaria segetalis polysaccharide color is faint yellow, and total sugar content is 66%.
Embodiment 5
It is an amount of to get the Vaccaria segetalis seed of frying after the process of preparing Chinese medicine, adds 5 times of amount distilled water, carries out microwave extraction, microwave power is 600w, extracts twice, and each extraction time is 10min, merge 2 times extracting solution, 150 order double-layer filter cloths filter, and the filtrate heating is concentrated into 1/3 of original volume; Stir and in concentrated extracting solution, to add 4 times of ethanol down, leave standstill 12h after, collecting precipitation obtains the Vaccaria segetalis crude polysaccharides.In the polysaccharide raw sediment, add dissolved in distilled water, make it abundant redissolution, add trichloroacetic acid to 1-10% concentration, leave standstill 3h after the stirring, the solution heating is concentrated into 1/3 of original volume, stirs to add 4 times of ethanol down in concentrated extracting solution, after leaving standstill 12h, collecting precipitation.Precipitation is with an amount of absolute ethanol washing 3 times, and the Vaccaria segetalis that obtains after the drying after the Deproteinization is made with extra care polysaccharide.The refining polysaccharide of this Vaccaria segetalis can further be prepared as various pharmaceutically acceptable compositionss and various dosage form.
Gained Vaccaria segetalis polysaccharide color is faint yellow, and total sugar content is 67%.
Embodiment 6
It is an amount of to get the Vaccaria segetalis seed, adds 5 times of amount distilled water, heating and refluxing extraction 3 times, and each extraction time is 1.5 hours, merges three times extracting solution, 150 order double-layer filter cloths filter, and behind the filtrate concentrate drying, obtain the Vaccaria segetalis crude polysaccharides.This Vaccaria segetalis crude polysaccharides can further be prepared as various pharmaceutically acceptable compositionss and various dosage form.
Gained Vaccaria segetalis polysaccharide color is faint yellow, and total sugar content is 40%.
Embodiment 7
It is an amount of to get the Vaccaria segetalis seed, adds 5 times of amount distilled water, carries out microwave extraction, and microwave power is 600w, extracts twice, and each extraction time is 10min, merges 2 times extracting solution, and 150 order double-layer filter cloths filter, and behind the filtrate concentrate drying, obtain the Vaccaria segetalis crude polysaccharides.This Vaccaria segetalis crude polysaccharides can further be prepared as various pharmaceutically acceptable compositionss and various dosage form.
Gained Vaccaria segetalis polysaccharide color is faint yellow, and total sugar content is 41%.
Embodiment 8
It is an amount of to get the Vaccaria segetalis seed of frying after the process of preparing Chinese medicine, adds 5 times of amount distilled water, heating and refluxing extraction 3 times, and each extraction time is 1.5 hours, merges three times extracting solution, the filtration of 150 order double-layer filter cloths behind the filtrate concentrate drying, obtains the Vaccaria segetalis crude polysaccharides.This Vaccaria segetalis crude polysaccharides can further be prepared as various pharmaceutically acceptable compositionss and various dosage form.
Gained Vaccaria segetalis polysaccharide color is faint yellow, and total sugar content is 35%.
Gained Vaccaria segetalis polysaccharide color is faint yellow, and total sugar content is 37%.
Get Vaccaria segetalis polysaccharide 20g, crushing screening, encapsulated, promptly get capsule.Every capsules contains Vaccaria segetalis polysaccharide 0.2g.
Embodiment 11
Get the about 20g of Vaccaria segetalis polysaccharide, add starch 15.0g, magnesium stearate 2.5g, Pulvis Talci 2.5g, the mixing grinding and sieving, tabletting, coating promptly gets tablet.Every contains Vaccaria segetalis polysaccharide 0.1g.
Embodiment 12
Get the about 20g of Vaccaria segetalis polysaccharide, it is an amount of to add water, and heating for dissolving becomes thick extractum.Add the 100g Icing Sugar, mix homogeneously, the granulation of sieving, drying, the back packing of sieving promptly gets granule.Every bag contains Vaccaria segetalis polysaccharide 0.2g.
Embodiment 13
It is an amount of to get PEG6000, heating and melting, and adding Vaccaria segetalis polysaccharide is an amount of, stirring and evenly mixing, filtered while hot splashes in the liquid coolant with 100 droplets/minute speed, is condensed into ball.Take out, wash ball, promptly get drop pill with liquid paraffin.
Above-mentioned dry run can adopt constant pressure and dry, drying under reduced pressure, spray drying or lyophilisation because of the purpose difference.Toxicity test, the effect experiment result of the Vaccaria segetalis polysaccharide of the employing embodiment of the invention 1 method preparation are as follows:
1, toxicity is observed:
Get 64 of Kunming mouses, random packet, employing is taken and is not taken the Vaccaria segetalis polysaccharide and carries out toxicity contrast experiment research.The result shows that Vaccaria segetalis polysaccharide group is under the dosage up to 500mg/kg, and is without any side effects, and by all no abnormal to the heart, liver, kidney, lung anatomic observation, and body weight all has increase.Experimental result sees Table 1:
Table 1 Vaccaria segetalis polysaccharide toxicity test
2, to the influence of urinary tract infection rat model
60 of SD mices, male and female have concurrently, be divided into 7 groups at random by body weight, be blank group (distilled water 10mL/kg), model group (distilled water 10mL/kg), SANJIN PIAN group (2.5g/kg), the basic, normal, high dosage group of Vaccaria segetalis polysaccharide (corresponding Vaccaria segetalis polysaccharide 0.5g/kg, 1.0g/kg, 2.0g/kg respectively).Vaccaria segetalis alcohol soluble substance group (2.0g/kg adopts the embodiment of the invention 1 method, and Vaccaria segetalis with the alcoholic solution concentrate drying, gets the Vaccaria segetalis alcohol soluble substance behind water extract-alcohol precipitation)
Except that the blank group, the previous day is prohibited water 15h in all the other zooperies, uses etherization, and is fixing, following stomach wall unhairing, and the about 3cm of the positive stomach wall of medisection is long, exposes the abdominal cavity, is threaded a needle to posterior abdominal wall place side respectively by both sides, left kidney ureter stage casing with wearing No. 4 line angle pins.Silk thread two is drawn outside the stomach wall, and (concentration is 6 * 10 to the about 0.6mL escherichia coli liquid of intravesical injection then
7G/mL), when flowing out, urethral orifice stops injection when seeing bacterium liquid.For improving the model success rate, short-time characteristic ligation ureter is sewed up the incision, and recovers drinking-water and feed material.Postoperative 24h removes the ureter ligature.All animals are according to dosage irritated the stomach relative medicine, every day 1 time, 7d continuously.
Measure following index: the urine amount of 24h after the administration of (1) mensuration last; (2) put to death animal, from bladder, extract urine 0.3mL, detect escherichia coli positive rate in the bladder urine.Result of the test sees Table 2～table 3.
Table 2 Vaccaria segetalis polysaccharide to urinary tract infection rat model urine amount influence the result (x ± s, n=10)
Annotate: compare with model group,
*P＜0.05,
*P＜0.01
By table 2 as seen, compare with the blank group, urinary tract infection model group rat urine amount obviously reduces; Compare with model group, Vaccaria segetalis alcohol soluble substance group rat urine amount does not have significant difference, and the basic, normal, high dosage group rat urine amount of Vaccaria segetalis polysaccharide has significant difference, its 24h urine amount obviously increases, show that the Vaccaria segetalis polysaccharide can obviously increase urinary tract infection rat model urine amount, improve the symptom of urinary tract infection rat model, and effect is better than the SANJIN PIAN group.Show that simultaneously the Vaccaria segetalis polysaccharide is the drug activity composition in the Vaccaria segetalis.
Table 3 Vaccaria segetalis polysaccharide is to the result that influences of rat urine escherichia coli positive rate
Annotate: compare with model group,
*P＜0.05,
*P＜0.01
By table 3 as seen, compare with the blank group, escherichia coli are checked total positives in the urinary tract infection model group rat urine; Compare with model group, the escherichia coli positive rate significantly reduces in the basic, normal, high dosage group rat urine of Vaccaria segetalis polysaccharide, show that the Vaccaria segetalis polysaccharide can obviously reduce escherichia coli positive rate in Buddhist nun's rate infection model difficult to understand rat urine, improve the infection symptoms of urinary tract infection rat model, and effect is better than the SANJIN PIAN group.Escherichia coli positive rate and model group do not have significant difference in the Vaccaria segetalis alcohol soluble substance group rat urine, show that the Vaccaria segetalis polysaccharide is the drug activity composition in the Vaccaria segetalis.
Vaccaria segetalis polysaccharide determination method of the present invention is for example following:
1. take by weighing the Vaccaria segetalis polysaccharide of an amount of embodiment of the invention 1-9 method preparation, wherein the Vaccaria segetalis polysaccharide of embodiment 1-5 method preparation directly adds 10mL water, and ultrasonic dissolution gets the Vaccaria segetalis polysaccharide solution; After the Vaccaria segetalis polysaccharide adding 20mL water of embodiment 6-9 method preparation fully dissolves, adopt the method for removing protein among the embodiment 1, be prepared into the refining polysaccharide of Vaccaria segetalis, add 10mL water again, ultrasonic dissolution gets the Vaccaria segetalis polysaccharide solution.The ultraviolet spectrophotometry analysis result has absorption at the 206nm place, does not have absworption peak more than the 230nm, shows not contain protein in the gained polysaccharide.Add phenolsulfuric acid reagent and be salmon pink in solution, add alpha-Naphthol-sulphate reagent and be purple, ninhydrin reaction is negative, identifies that its main component is a polysaccharide.
1. take by weighing the Vaccaria segetalis polysaccharide of an amount of embodiment of the invention 1-9 method preparation, wherein the Vaccaria segetalis polysaccharide of embodiment 1-5 method preparation directly adds 10mL water, and ultrasonic dissolution gets the Vaccaria segetalis polysaccharide solution; After the Vaccaria segetalis polysaccharide adding 20mL water of embodiment 6-9 method preparation fully dissolves, adopt the method for removing protein among the embodiment 1, be prepared into the refining polysaccharide of Vaccaria segetalis, add 10mL water again, ultrasonic dissolution gets the Vaccaria segetalis polysaccharide solution.The ultraviolet spectrophotometry analysis result has absorption at the 206nm place, does not have absworption peak more than the 230nm, shows not contain protein in the gained polysaccharide.Add phenolsulfuric acid reagent and be salmon pink in solution, add alpha-Naphthol-sulphate reagent and be purple, ninhydrin reaction is negative, identifies that its main component is a polysaccharide.
3. the assay method of total sugar amount: precision gets that the anhydrous glucose reference substance is an amount of, and precision takes by weighing, and adds water and makes the solution that every 1ml contains anhydrous glucose 0.6mg, promptly.The accurate reference substance solution 1.0,1.5,2.0,2.5,3.0,3.5,4.0,4.5,5.0 of drawing is put the 50mL volumetric flask respectively, adds water to scale, shakes up.Above-mentioned each solution 2mL is in tool plug test tube in accurate absorption, adds 4% phenol 1.0mL, shakes up, add concentrated sulphuric acid 7.0mL rapidly, shake up, insulation is 30 minutes in 40 water-baths, take out, put in the ice-water bath 5 minutes, take out, with the corresponding reagent is blank, according to the ultraviolet-visible light photometry, measure absorbance at the wavelength place of 490nm, be vertical coordinate with the absorbance, concentration is abscissa, the drawing standard curve.
The Vaccaria segetalis polysaccharide sample of getting embodiment of the invention 1-12 is an amount of, is mixed with solution by the compound method of titer, draws 2ml in tool plug test tube, add 4% phenol 1.0mL and shake up, add concentrated sulphuric acid 7.0mL rapidly, shake up, insulation is 30 minutes in 40 water-baths, take out, put in the ice-water bath 5 minutes, take out, with the corresponding reagent is blank, press the ultraviolet-visible light photometry, measure absorbance, calculate total sugar content according to standard curve at the wavelength place of 490nm.
Claims (8)
1. a Vaccaria segetalis polyoses extract is characterized in that: obtained by the extraction of Caryophyllaceae Vaccaria plant Vaccaria segetalis (Vaccaria segetalis (Neck.) Garcke) raw material; Described Vaccaria segetalis raw material is the Vaccaria segetalis seed.
2. Vaccaria segetalis polyoses extract according to claim 1, described Vaccaria segetalis seed is the Vaccaria segetalis seed after concocting.
3. Vaccaria segetalis polyoses extract according to claim 1 and 2, described Vaccaria segetalis polyoses extract is faint yellow, total sugar content is 30-90wt%.
4. the purposes of any one described Vaccaria segetalis polyoses extract among the claim 1-3 is characterized in that: be used to prepare the urinary tract infection medicine, indication is urgent micturition, frequent micturition or the dysurea that urinary tract infection or diabetes cause.
5. the extracting method of any one described Vaccaria segetalis polyoses extract among the claim 1-3: it is characterized in that: may further comprise the steps: get the Vaccaria segetalis raw material, the distilled water that adds 5-15 times of weight, heating and refluxing extraction 3-5 time or microwave extraction 2-3 time, merge extractive liquid,, filter, the filtrate heating concentrates; Adding ethanol to concentration of alcohol under stirring in concentrated extracting solution is 50-95%, leaves standstill, and collecting precipitation obtains the Vaccaria segetalis crude polysaccharides.
6. the extracting method of Vaccaria segetalis polyoses extract according to claim 5, further comprising the steps of: as in described Vaccaria segetalis crude polysaccharides, to add trichloroacetic acid or Sevag reagent protein precipitation; Perhaps use proteasome degradation albumen, adopt ethanol acetone precipitation, ultrafiltration or dialysis to reclaim polysaccharide then, be drying to obtain.
7. a pharmaceutical composition that is used for the treatment of urinary tract infection is characterized in that being made up of any one described Vaccaria segetalis polyoses extract and acceptable accessories among the claim 1-3.
8. the pharmaceutical composition that is used for the treatment of urinary tract infection according to claim 7 is characterized in that: the content of described Vaccaria segetalis polyoses extract is 1-99.9wt%, and adjuvant is 0.1-99wt%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104710540A (en) * | 2015-03-11 | 2015-06-17 | 淮阴工学院 | Preparation method of vaccaria segetalis polysaccharide |
| CN106692255A (en) * | 2017-01-12 | 2017-05-24 | 淮安首考科技有限公司 | Vaccaria segetalis polysaccharide hydrolysate and medical application thereof |
-
2011
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| Title |
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| 冯磊等,1: "王不留行抑制血管形成作用的研究 ", 《中药材》 * |
| 冯磊等,1: "王不留行抑制血管形成作用的研究", 《中药材》, vol. 32, no. 08, 25 August 2009 (2009-08-25), pages 1256 - 1259 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104710540A (en) * | 2015-03-11 | 2015-06-17 | 淮阴工学院 | Preparation method of vaccaria segetalis polysaccharide |
| CN106692255A (en) * | 2017-01-12 | 2017-05-24 | 淮安首考科技有限公司 | Vaccaria segetalis polysaccharide hydrolysate and medical application thereof |
| CN106692255B (en) * | 2017-01-12 | 2020-07-21 | 淮安首考科技有限公司 | Brassica oleracea polysaccharide hydrolysate and medical application thereof |
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