CN102220392B - Method for producing chitosan by moulds cultured in bean curd yellow water - Google Patents
Method for producing chitosan by moulds cultured in bean curd yellow water Download PDFInfo
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- CN102220392B CN102220392B CN 201110103461 CN201110103461A CN102220392B CN 102220392 B CN102220392 B CN 102220392B CN 201110103461 CN201110103461 CN 201110103461 CN 201110103461 A CN201110103461 A CN 201110103461A CN 102220392 B CN102220392 B CN 102220392B
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Abstract
The invention discloses a method for producing chitosan by moulds cultured in bean curd yellow water. In the method, 30 to 60 percent of bean curd yellow water is used as a culture medium; at a natural pH value and under a sterilized or non-sterilized condition, suspension of spores of moulds is inoculated in an inoculation amount of 3 to 5 percent; and the spores of mould are cultured at 28 to 32 DEG C with oscillating at a speed of 150 to 180rpm for about 50 to 60 hours, filtering and watering are performed, mycelia are collected, and the dry weight of the mycelia is 3 to 8 grams per liter. Meanwhile, the chemical oxygen demand (COD) Cd removal rate may reach over 90 percent. When chitosan are extracted from thalli by a diluted acid and diluted alkali treatment process, the chitosan yield can reach over 7 percent. The quality of chitosan produced by the method compares with chitosan extracted from shrimps and crabs. When the method is used, the production cost is low, the production process is simple, the product quality is high, safety and environment friendliness are ensured, and the food waste water is treated while the chitosan product is produced.
Description
Technical field
The invention belongs to biological technical field, particularly relate to a kind of bean curd yellow water that utilizes and cultivate the method that mould produces chitosan.
Background technology
Chitosan is a kind of polysaccharide, is the de-acetyl chitin product, is with a wide range of applications.Traditional industry is produced chitosan and is to use concentrated acid, concentrated base to extract from shrimp, crab shell, and not only contaminate environment, and raw material sources also is subjected to the restriction in season and area.The Production by Microorganism Fermentation chitosan is a new chitosan production approach, raw material production is not subjected to the region season limit, and leaching process uses diluted acid, diluted alkaline, reduces the pollution to environment, and be expected to reduce production costs, be a kind of chitosan production method that gets a good eye with using value.Therefore from the eighties in 20th century day wood and the U.S. successively begin one's study with the method production chitosan of microbial fermentation, domestic since early 1990s also this side and research.Research emphasis mainly concentrates in the seed selection and Optimum of culture medium of bacterial strain at present, owing to become wood higher with the fermentative Production chitosan, is difficult to carry out large-scale industrial production.Contain more rich carbon source and nitrogenous source at foodstuff production waste water, can provide nutrition for mould-growth, be the culture medium culturing mould with it, not only can obtain a large amount of fungal hyphae bodies, in order to extract chitosan, also handled food wastewater, when having reduced the chitosan production cost, reached the treatment of wastes with processes of wastes against one another, turn waste into wealth, the purpose of safety and environmental protection.
Summary of the invention
The objective of the invention is to propose a kind of bean curd yellow water that utilizes and cultivate the method that mould produces chitosan, its outward appearance of chitosan that this method is produced is the pale powder shape, and moisture content is less than 11%, and ash oontent is lower than 0.4%, and deacetylation is more than 90%.
The objective of the invention is to be achieved through the following technical solutions:
A kind of bean curd yellow water that utilizes is cultivated the method that mould produces chitosan, comprises the steps:
1) preparation of seed liquor:
Under sterilising conditions, the bean curd yellow water with 40% is substratum, and the inoculum size by 5% inserts mould spores suspension, 30
0The velocity fluctuation of C, 180rpm is cultivated 24h and is got seed liquor;
2) mycelial fermentative production:
Be substratum with the bean curd yellow water, at waste water nature pH, under the condition of non-sterilization by 5% inoculum size inoculation seed liquor, in 28-35
0After the velocity fluctuation of C, 150-200rpm was cultivated 36h, filtration, washing obtained mycelium;
3) extraction of chitosan:
With step 2) mycelium that obtains smashs to pieces in thalline: the ratio of alkali lye=1:10 (w/v) adds sodium hydroxide solution, and microwave treatment 25min, centrifuge washing are precipitated to supernatant liquor and are neutral; In the precipitation by precipitation: acetic acid=1:15(w/v) adds acetic acid solution, handles centrifugation 4 hours down for 100 ℃; It is 8-10 that supernatant liquor is regulated the pH value, obtains white flocks, centrifugal, and it is neutral that deionized water washing precipitation to supernatant liquor is; Be deposited in 50C
0Following vacuum-drying obtains pale powder shape chitosan.
Described a kind of bean curd yellow water that utilizes is cultivated the method that mould produces chitosan, and its described substratum is not for adding the bean curd yellow water of any nutritive substance, and its concentration is 30%-60%.
Described a kind of bean curd yellow water that utilizes is cultivated the method that mould produces chitosan, and it carries out under sterilization or non-sterilizing condition, and direct inoculation mould spores suspension under sterilising conditions obtains the fungal hyphae body behind the cultivation 50-60h.
Described a kind of bean curd yellow water that utilizes is cultivated the method that mould produces chitosan, and its used concentration of sodium hydroxide solution is 10%, and the microwave firepower is 400w, and acetic acid concentration is 5%.
Advantage of the present invention and effect are:
(1) cost is low.Need not to be added into any other nutritive substance, under non-sterilizing condition, directly use certain density bean curd yellow water to produce chitosan as the culture medium culturing mould, greatly reduce production cost.
(2) be suitable for large-scale production.Compare with additive method, raw material sources are not subjected to season and territorial restrictions, because bean product production has realized scale, so wastewater source is secure, can adopt fermentor tank or reaction tank to carry out the cultivation of mould under the condition of continuously stirring, the realization suitability for industrialized production is highly advantageous to.
(3) safety non-pollution.Be that substratum is safer with the food wastewater, when obtaining thalline, handled food wastewater.Adopting microwave method to extract chitosan simultaneously greatly reduces acid base concentration, has reduced the soda acid consumption, has shortened preparation time.Reached low cost, less contamination, the treatment of wastes with processes of wastes against one another, turned waste into wealth, the purpose of safety and environmental protection.
Its outward appearance of chitosan that this method is produced is the pale powder shape, and moisture content is less than 11%, and ash oontent is lower than 0.4%, and deacetylation is more than 90%.Can be used for fields such as foodstuffs industry, medical and health, light and textile industries, agricultural and environment.
Embodiment
Below by embodiment technology of the present invention is described further.
Embodiment 1:
In the 250mL Erlenmeyer flask, add 40mL bean curd yellow water and 60mL tap water, natural pH, the inoculum size by 5% under the condition of sterilization is inoculated the mould spores suspension, in 30
0The velocity fluctuation of C, 180rpm is cultivated 50h, and filtration washing is collected mycelium, and mycelium dry weight reaches 6.2g/L.Culture medium C OD clearance can reach more than 95.1%.
Embodiment 2:
Add 40mL bean curd yellow water and 60mL tap water in the 250mL Erlenmeyer flask, regulate pH=4, the mould spores suspension after pressing 5% inoculum size inoculation culture 24h under the condition of non-sterilization is in 30
0The velocity fluctuation of C, 180rpm is cultivated 36h, and filtration washing is collected mycelium, and mycelium dry weight reaches 5.5/L.Culture medium C OD clearance reaches 93.1%.
Embodiment 3
Get the fungal hyphae body 150g(weight in wet base of cultivating by the described method of embodiment 1-2) smash to pieces, add 10% sodium hydroxide solution by the ratio of thalline: alkali lye=1:10 (w/v), 400w microwave treatment 25min, centrifuge washing are precipitated to supernatant liquor and are neutrality.In precipitation: the ratio of acetic acid=1:15(w/v) adds 5% acetic acid solution in precipitation, handled centrifugation 4 hours down for 100 ℃.Supernatant liquor is regulated the pH value and is 8-10, and is centrifugal, and it is neutral that deionized water washing precipitation to supernatant liquor is.Be deposited in 50C
0Following vacuum-drying obtains pale powder shape chitosan, and the chitosan yield is 7.2%.Deacetylation is 90.2%, moisture 10.2%, ash content 0.4%.
Claims (1)
1. one kind is utilized the bean curd yellow water to cultivate the method that mould produces chitosan, it is characterized in that comprising the steps:
1) preparation of seed liquor:
Under sterilising conditions, the bean curd yellow water with 40% is substratum, and the inoculum size by 5% inserts mould spores suspension, 30
0The velocity fluctuation of C, 180rpm is cultivated 24h and is got seed liquor;
2) mycelial fermentative production:
Be substratum with the bean curd yellow water, at waste water nature pH, under the condition of non-sterilization by 5% inoculum size inoculation seed liquor, in 28-35
0After the velocity fluctuation of C, 150-200rpm was cultivated 36h, filtration, washing obtained mycelium;
3) extraction of chitosan:
With step 2) mycelium that obtains smashs to pieces in thalline: the ratio of alkali lye=1:10 (w/v) adds sodium hydroxide solution, and microwave treatment 25min, centrifuge washing are precipitated to supernatant liquor and are neutral; In the precipitation by precipitation: acetic acid=1:15(w/v) adds acetic acid solution, handles centrifugation 4 hours down for 100 ℃; It is 8-10 that supernatant liquor is regulated the pH value, obtains white flocks, centrifugal, and it is neutral that deionized water washing precipitation to supernatant liquor is; Be deposited in 50C
0Following vacuum-drying obtains pale powder shape chitosan; Used concentration of sodium hydroxide solution is 10%, and the microwave firepower is 400w, and acetic acid concentration is 5%.
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| CN 201110103461 CN102220392B (en) | 2011-04-25 | 2011-04-25 | Method for producing chitosan by moulds cultured in bean curd yellow water |
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| CN 201110103461 CN102220392B (en) | 2011-04-25 | 2011-04-25 | Method for producing chitosan by moulds cultured in bean curd yellow water |
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| CN102220392B true CN102220392B (en) | 2013-08-28 |
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| CN102578096B (en) * | 2012-01-30 | 2013-11-27 | 浙江大学 | Application of chitosin control of clubroot of plants |
| US11229095B2 (en) | 2014-12-17 | 2022-01-18 | Campbell Soup Company | Electromagnetic wave food processing system and methods |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101797497A (en) * | 2010-03-26 | 2010-08-11 | 沈阳化工学院 | Mould biosorbent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101797497A (en) * | 2010-03-26 | 2010-08-11 | 沈阳化工学院 | Mould biosorbent and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 犁头霉菌培养制备壳聚糖;谢德明;《暨南大学学报( 自然科学版)》;20000630;第21卷(第3期);91-94 * |
| 谢德明.犁头霉菌培养制备壳聚糖.《暨南大学学报( 自然科学版)》.2000,第21卷(第3期),91-94. |
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