CN102220322A - Molecular markers for controlling close linkage of main active quantitative trait loci (QTL) associated with ear thickness of corn - Google Patents
Molecular markers for controlling close linkage of main active quantitative trait loci (QTL) associated with ear thickness of corn Download PDFInfo
- Publication number
- CN102220322A CN102220322A CN2011101650094A CN201110165009A CN102220322A CN 102220322 A CN102220322 A CN 102220322A CN 2011101650094 A CN2011101650094 A CN 2011101650094A CN 201110165009 A CN201110165009 A CN 201110165009A CN 102220322 A CN102220322 A CN 102220322A
- Authority
- CN
- China
- Prior art keywords
- corn
- molecule marker
- primer
- qtl
- mealie
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses molecular markers Umc1861 and Umc2088 for controlling close linkage of main active quantitative trait loci (QTL) associated with ear thickness of corn. The molecular markers can be used for genotype detection of corn and derivative varieties or lines thereof for determining the ear thickness of the varieties or lines and improving selection accuracy and breeding efficiency.
Description
Technical field
The present invention relates to corn breeding and biology field,, thereby utilize it that mealie is slightly carried out molecular marker assisted selection by the molecule marker of utilization with the thick main effect QTL compact linkage of mealie.
Background technology
Corn is important grain ration and fodder crop, and China's maize yield is very big to the grain security influence of country.The theory and practice of corn breeding proves, the output of self-mating system and the output of its cross-fertilize seed closely related (Richey, 1946).And under specific planting density, the output of corn hybrid seed unit surface is made up of corn list tassel seed output and spike number, and the diameter of single tassel seed output and fringe, be the thick positive correlation of fringe, therefore, thick proterties of fringe and the remarkable positive correlation of corn yield (Liu Jilin, 2000), verify the thick hereditary mechanism that forms of fringe and help to illustrate the hereditary mechanism that the corn yield proterties forms, the cross-fertilize seed that seed selection has large spike is a kind of feasible solution that promotes China's corn yield per unit.The thick proterties of mealie is subjected to such environmental effects less, and therefore, this proterties is an ideal molecular breeding improvement proterties.Mealie slightly is the crucial proterties of decision corn yield, and it is a kind of quantitative character, is subjected to the control of a plurality of genes.The slightly relevant QTL of existing a plurality of localized fringes obtained the location (
Www.gramene.org).
The research of dna molecular marker starts from 1980, Bostein etc. (1980) have at first proposed to utilize the RFLP mark to make up genetic linkage map, decades subsequently, occurred tens kinds of molecule markers such as SSR, AFLP, RAPD, CAPS, SCAR, SNP again in succession, enjoy the geneticist to favor because molecule marker has following advantage: (1) is not subjected to season, environment, genetic expression whether restriction; (2) polymorphism height exists abundant allelic variation; (3) quantity is abundant, and polymorphism spreads all over whole genome; (4) many molecule marker performance codominances can be differentiated the heterozygous genes type that isozygotys, and complete information is provided.
The QTL location is by the phenotypic number of quantitative character and the association analysis between molecule marker, promptly when mark and specific trait are chain, there is significant difference between the phenotypic number of different marker gene type individualities, determine position and the effect of each quantitative trait locus on karyomit(e) with this, even make effect (Tanksley, 1993) mutually between each QTL.In the qtl analysis process, at first select two influencing the parent material that there are differences between the allelotrope of objective trait, utilize these two parents to create a big mapping population then, the genotype of each individual phenotypic number and multidigit point in the analysis colony, a polymorphism genetic map that makes up between two materials is used for genetic analysis, determine the relative position of QTL on karyomit(e), and estimate the relevant genetic parameter (Mauricio, 2001) of QTL.
The molecule marker report linked with the thick main effect QTL of control mealie do not arranged at present as yet.
Summary of the invention
An object of the present invention is to provide the molecule marker of control mealie thick main effect QTL and, can predict that by detecting these molecule markers mealie is thick, accelerate the breeding progress of corn in the application of molecular marker assisted selection.
One aspect of the present invention relates to a kind of molecule marker of controlling the thick main effect QTL compact linkage of mealie, it is characterized in that, uses maize dna, adopts the PCR primer right respectively:
Left end primer 5 ' CAAAAGGTTTTGAATTAAAATATCCGA and right-hand member primer 5 ' CAAAGGCAAGAACTCTAGCATGAA
Left end primer 5 ' ACGACAAGAAGGAGGCCAAAG and right-hand member primer 5 ' CAAGTAGATCGATCGAGCAGCAG
Carry out pcr amplification, amplified production obtains molecule marker Umc1861 and umc2088 respectively after separating by gel electrophoresis.
In a preferred embodiment of the present invention, described gel is meant 6% polyacrylamide gel and 1% agarose gel electrophoresis.
In a preferred embodiment of the present invention, its feature is yellow C and/or is permitted 178 at described corn variety.
The present invention also relates to the application of above-mentioned molecule marker on the other hand, it is characterized in that: molecule marker Umc1861 and umc2088 are used for the genotype detection of corn, to judge that mealie is thick.
In a preferred implementation of application of the present invention, with yellow C and/or permitted 178 for male parent or maternal and other corn hybridizations and multiply to F2 for more than, extract their DNA, primer with Umc1861 and umc2088 carries out polymerase chain reaction (PCR) to these DNA then, pass judgment on these 2 and mark whether to exist, come the fringe of forecasting institute acquisition kind or strain thick.
Description of drawings
Fig. 1 is SSR mark umc1861, umc2088 to the yellow C of parent and is permitted 178 individual plants, 6% polyacrylamide gel electrophoresis detected result;
Fig. 2 is SSR mark umc1861, umc2088 to the yellow C of parent and is permitted 178 individual plants, 1% agarose gel electrophoresis detected result;
Fig. 3 is SSR mark umc2088 to the yellow C of parent, is permitted 178 and F2 colony random choose individual plant 6% polyacrylamide gel electrophoresis result;
Fig. 4 is SSR mark umc1861, umc2088 and the thick main effect QTL qED2a of fringe linkage map (map unit is cm).
Embodiment
Further set forth the present invention below in conjunction with specific embodiment.The experimental technique of unreceipted actual conditions among the embodiment, usually according to normal condition, " molecular cloning " third edition for example, or the condition of being advised according to reagent manufacturer.
The yellow C of parent and permitted 178 hybridization and obtain F1 generation, the F1 selfing obtains F2 generation.F2 utilizes the small quality fast extraction method (Du Hewei etc., 2004) of maize dna for individual plant seedling phase (5 true leaf its after) blade that takes a morsel, and extracts F2 individual plant genomic dna.
Adopt following primer increase yellow C, permitted 178 and the F2 DNA isolation, utilize 6% polyacrylamide gel and 1% agarose gel electrophoresis to obtain molecule marker umc1861:
Left end primer 5 ' CAAAAGGTTTTGAATTAAAATATCCGA;
Right-hand member primer 5 ' CAAAGGCAAGAACTCTAGCATGAA
Adopt following primer increase yellow C, permitted 178 and the F2 DNA isolation, utilize 6% polyacrylamide gel and 1% agarose gel electrophoresis to obtain molecule marker umc2088:
Left end primer 5 ' ACGACAAGAAGGAGGCCAAAG
Right-hand member primer 5 ' CAAGTAGATCGATCGAGCAGCAG
The F2 segregating population individual plant of amplification maize elite inbred line agricultural university 108 (yellow C * permitted 178), itself and place corn article one karyomit(e) 2.04bin section identify the thick main effect QTL of mealie, and contribution rate is 13.9%.
Utilize above molecule marker umc1861, umc2088, scanning F2 segregating population carries out gene type assay to each F2 individual plant.The PCR system: template DNA 30-50ng, primer 5umol/L, dNTPs are 200umol/L, 2mmol/L MgCl2,1X Taq Buffer, 0.5U TaqDNA polysaccharase, the total reaction system of 10ul.The TaqDNA polysaccharase is purchased in Shanghai life worker biotechnology company limited.The PCR response procedures is: 94 ℃ of 5min; 35 circulations, 94 ℃ of 30sec, 58 ℃ of 45sec, 72 ℃ of 45sec; 72 ℃ of 5min.The detection method of above-mentioned mark is improved 6% denaturing polyacrylamide gel detection.1XTBE is an electrophoretic buffer, the firm power of 80W, electrophoresis 1 hour.Carry out silver behind the electrophoresis and dye, the method that silver dyes is: utilize ultrapure washing one minute; 1.5L it is staining fluid that ultrapure water adds 3g AgNO3, dyes 10 minutes; Ultrapure water cleaned 30 seconds; Colour developing liquid is the 1.5L ultrapure water, adds 30gNaOH, 0.6g anhydrous Na2CO3 and 7.5ml formaldehyde, develops the color 10 minutes, uses flushing with clean water.
Phenotype is investigated to regaining corn ear in ripe back, amount fringe diameter after the artificial threshing.Utilize MapMaker software to carry out linkage map and make up, WinQTLCart 2.5 composite interval mapping methods are carried out the scanning location of QTL.
The present invention utilizes the yellow C of good self-mating system and is permitted the F2 that 178 hybridization obtain and identifies the thick QTL qED2a of fringe for segregating population, utilize the acquisition RIL of backcrossing of many generations in F2 generation, utilize corn IBM2008 molecule marker umc1861, umc2088 screening exchange individual plant, utilize the BAC sequence of the corn inbred line B73 of corn gene group order-checking plan release, amplification SSR site, screening is carried out the exploitation of mark in the yellow C and the SSR site of being permitted to exist between 178 polymorphism.Based on above result, utilized 152 parts of RILs in 2009, qED2a is navigated in 5.0cM and the 4.2cM scope, the molecule marker linkage map is as shown in Figure 4.
The present invention uses the yellow C of good self-mating system and is permitted F2 that 178 hybridization obtain and carry out the location of the thick QTL of fringe for segregating population, the molecule marker of exploitation and the thick main effect QTL compact linkage of fringe.The genetic stocks that the present invention uses is for yellow C and permitted the recombinant inbred strain that F2 family segregating population that 178 hybridization obtain and continuous multi-generation selfing obtain.Parent's fringe of 178 perhaps slightly is 3.3-3.6cm, and the fringe of the yellow C of parent slightly is 4.0-4.6cm
2004--2005, with yellow C with to be permitted the F2 that 178 hybridization obtain be material for segregating population, carry out in Zhengzhou City, Henan Province and two places, Xinzheng City that spike length, fringe are thick, tassel row number, a row grain number, the fringe grain is heavy and the examination of 6 yield traitses such as 100-grain weight, 53 QTL have been identified altogether, wherein all detected QTL has 5 in two places, shows the mutual work of a large amount of QTL and environment.At the Umc1861-umc2088 of the second chromosomal 2.04bin (LOD=5.4, R
2=13.9%) section, multiple spot test confirm to have identified 1 with fringe slightly, relevant main effect QTL such as output, this QTL plays synergism by the site from yellow C.
2008,2009, carried out the QTL checking of RIL in Zhengzhou, Henan and Xunxian, 2 positioning result navigated to QTL between the Umc1861-umc2088 jointly in 2 years, and its genetic distance is respectively 5.0cM and 4.2cM, and accompanying drawing 1 is molecule marker and the chain situation of QTL.This section QTL effect is respectively Zhengzhou R in 2008
2=9.5%, Xunxian R
2=11.6%; Zhengzhou R in 2009
2=9.6%, Xunxian R
2=12.4% (WinCatographer 2.5).
Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (5)
1. a molecule marker of controlling the thick main effect QTL compact linkage of mealie is characterized in that, uses maize dna, adopts the PCR primer right respectively:
Left end primer 5 ' CAAAAGGTTTTGAATTAAAATATCCGA and right-hand member primer 5 ' CAAAGGCAAGAACTCTAGCATGAA
Left end primer 5 ' ACGACAAGAAGGAGGCCAAAG and right-hand member primer 5 ' CAAGTAGATCGATCGAGCAGCAG
Carry out pcr amplification, amplified production obtains molecule marker Umc1861 and umc2088 respectively after separating by gel electrophoresis.
2. the molecule marker of the thick main effect QTL compact linkage of control mealie according to claim 1 is characterized in that: described gel is meant 6% polyacrylamide gel and 1% agarose gel electrophoresis.
3. the molecule marker of the thick main effect QTL compact linkage of control mealie according to claim 1, its feature is yellow C and/or is permitted 178 at described corn variety.
4. application as any described molecule marker of claim 1 to 3 is characterized in that: molecule marker Umc1861 and umc2088 are used for the genotype detection of corn, to judge that mealie is thick.
5. the application of molecule marker according to claim 3, it is characterized in that: with yellow C and/or permitted 178 for male parent or maternal and other corn hybridizations and multiply to F2 for more than, extract their DNA, primer with Umc1861 and umc2088 carries out polymerase chain reaction (PCR) to these DNA then, pass judgment on these 2 and mark whether to exist, come the fringe of forecasting institute acquisition kind or strain thick.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101650094A CN102220322A (en) | 2011-06-20 | 2011-06-20 | Molecular markers for controlling close linkage of main active quantitative trait loci (QTL) associated with ear thickness of corn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101650094A CN102220322A (en) | 2011-06-20 | 2011-06-20 | Molecular markers for controlling close linkage of main active quantitative trait loci (QTL) associated with ear thickness of corn |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102220322A true CN102220322A (en) | 2011-10-19 |
Family
ID=44777026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011101650094A Pending CN102220322A (en) | 2011-06-20 | 2011-06-20 | Molecular markers for controlling close linkage of main active quantitative trait loci (QTL) associated with ear thickness of corn |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102220322A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016048686A1 (en) * | 2014-09-23 | 2016-03-31 | E. I. Du Pont De Nemours And Company | Compositions and methods for selecting maize plants with increased ear weight and increased yield |
| CN106148499A (en) * | 2015-04-17 | 2016-11-23 | 中国农业大学 | The molecular marker of Semen Maydis panicled characters hybrid vigor main effect QTL and application thereof |
| CN106906301A (en) * | 2017-04-26 | 2017-06-30 | 江苏省农业科学院 | Molecular labeling and its application with corn row grain number main effect QTL compact linkage |
| CN112646913A (en) * | 2020-12-25 | 2021-04-13 | 华中农业大学 | A pair of molecular marker primers closely linked with corncob coarse QTL ZmED3 and application thereof |
-
2011
- 2011-06-20 CN CN2011101650094A patent/CN102220322A/en active Pending
Non-Patent Citations (6)
| Title |
|---|
| 《中国农业科学》 20071130 刘宗华 等 氮胁迫和正常条件下玉米穗部性状的QTL 分析 第2409-2417页 1-5 第40卷, 第11期 * |
| HUI-LING XIE ET AL: "Genetic basis of nutritional content of stover in maize under low nitrogen conditions", 《EUPHYTICA》 * |
| MMP: "umc1861", 《WWW.MAIZEGDB.ORG》 * |
| MMP: "umc2088", 《WWW.MAIZEGDB.ORG》 * |
| 刘宗华 等: "氮胁迫和正常条件下玉米穗部性状的QTL 分析", 《中国农业科学》 * |
| 孙海艳 等: "玉米株型性状的QTL定位", 《西南大学学报( 自然科学版) 》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016048686A1 (en) * | 2014-09-23 | 2016-03-31 | E. I. Du Pont De Nemours And Company | Compositions and methods for selecting maize plants with increased ear weight and increased yield |
| US10316370B2 (en) | 2014-09-23 | 2019-06-11 | E I Du Pont De Nemours And Company | Compositions and methods for selecting maize plants with increased ear weight and increased yield |
| CN106148499A (en) * | 2015-04-17 | 2016-11-23 | 中国农业大学 | The molecular marker of Semen Maydis panicled characters hybrid vigor main effect QTL and application thereof |
| CN106148499B (en) * | 2015-04-17 | 2019-06-04 | 中国农业大学 | The molecular labeling of corn panicled characters hybrid vigour main effect QTL and its application |
| CN106906301A (en) * | 2017-04-26 | 2017-06-30 | 江苏省农业科学院 | Molecular labeling and its application with corn row grain number main effect QTL compact linkage |
| CN112646913A (en) * | 2020-12-25 | 2021-04-13 | 华中农业大学 | A pair of molecular marker primers closely linked with corncob coarse QTL ZmED3 and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jorge et al. | Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) | |
| ES2971826T3 (en) | Compositions and procedures for peronospora resistance in spinach | |
| US9113608B2 (en) | Methods and compositions for selecting soybean plants resistant to phytophthora root rot | |
| US8637729B2 (en) | F. oxysporum f.sp. melonis race 1,2-resistant melons | |
| WO2020036950A1 (en) | Molecular markers for blackleg resistance gene rlm1 in brassica napus, and methods of using the same | |
| US8530723B2 (en) | Markers associated with resistance to Aphis glycines and methods of use therefor | |
| CN102220322A (en) | Molecular markers for controlling close linkage of main active quantitative trait loci (QTL) associated with ear thickness of corn | |
| CA2923463C (en) | Molecular markers for blackleg resistance gene rlm2 in brassica napus, and methods of using the same | |
| US20130254927A1 (en) | Molecular markers for low palmitic acid content in sunflower (helianthus annus), and methods of using the same | |
| CA3069945A1 (en) | Anthracnose resistant alfalfa plants | |
| KR20220007592A (en) | Powdery Mildew Resistant Capsicum Plants | |
| AU2014268142B2 (en) | Disease resistance loci in onion | |
| CN106834281A (en) | SSR marker with black streaked dwarf virus of rice Resistance QTL close linkage and its application on No. 4 chromosomes | |
| US10793918B2 (en) | Molecular markers for blackleg resistance in canola and methods of using the same | |
| US20170150693A1 (en) | Methods and compositions for producing sorghum plants with anthracnose resistance | |
| US20210315177A1 (en) | Molecular markers for blackleg resistance gene rlm7 in brassica napus, and methods of using the same | |
| CN104004758A (en) | Molecular markers interlinked with main effect QTL (Quantitative Trait Locus) of photoperiod sensitivity of corn and application of molecular markers | |
| Yuskianti et al. | Detection of pollen flow in the seedling seed orchard of Acacia mangium using DNA marker | |
| US10883148B2 (en) | Molecular markers associated with Orobanche resistance in sunflower | |
| CN105385772A (en) | Primer used for screening wheat brown leaf rust resisting QTL and application thereof | |
| CN106834425A (en) | The anti-black streak dwarf site qRBSDV17 of rice varieties Zhejiang round-grained rice 5 and its molecule labelling method | |
| Liu | Genome analysis of trembling aspen and bigtooth aspen using molecular genetic markers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20111019 |