CN102220245A - Aspergillus nomius SGFA1 and use of aspergillus nomius SGFA1 in degradation of formaldehyde - Google Patents
Aspergillus nomius SGFA1 and use of aspergillus nomius SGFA1 in degradation of formaldehyde Download PDFInfo
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- CN102220245A CN102220245A CN 201110122361 CN201110122361A CN102220245A CN 102220245 A CN102220245 A CN 102220245A CN 201110122361 CN201110122361 CN 201110122361 CN 201110122361 A CN201110122361 A CN 201110122361A CN 102220245 A CN102220245 A CN 102220245A
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Abstract
The invention discloses aspergillus nomius SGFA1 and a use of the aspergillus nomius SGFA1 in degradation of formaldehyde. The preservation register number of the aspergillus nomius SGFA1 provided by the present invention is CGMCC No.4538. The aspergillus nomius SGFA1 provided by the present invention can grow through adopting the formaldehyde as sole carbon source under an aerobic condition, and simultaneously degrade the formaldehyde. With the present invention, the formaldehyde having a concentration of 80 mmol/L can be completely degraded in 7 days under a pure culture condition. The aspergillus nomius SGFA1 is expected to play an important role in biological treatment of industrial wastewater.
Description
Technical field
The present invention relates to red silk ribbon attached to an official seal or a medal aspergillus SGFA1 and the application in degradation of formaldehyde thereof.
Background technology
Formaldehyde is a kind of colourless at normal temperatures, and the gas of intense stimulus smell is arranged, soluble in water, pure and mild ether, and the form with the aqueous solution occurs usually.Chemical reactions such as addition, oxidation, reduction, polymerization easily take place in formaldehyde, have the water-soluble of height, can highly react with biomacromolecule, formaldehyde absorbs in the contact site or degraded, enter human body after, the protein and the nucleic acid reaction of main and human body: cause the reparation of commissure, DNA and protein commissure in cell nucleus gene sudden change, the dna single chain, inhibition dna damage, combine with amino, change proteinic internal structure and solidify the eubolism of upset human body cell, thereby generation lethality.The long-time formaldehyde gas that sucks, the immunity system but liver injury, kidney, blood system, Digestive tract, respiratory system, central nervous system are unified, women, pregnant woman's Long contact time low concentration formaldehyde gas, can cause menoxenia, fetal anomaly, the reduction of neonatal immunity power, physique to descend, intelligence growth produces obstacle.Formaldehyde is poisonous, teratogenesis, and formaldehyde also has the intensive carcinogenesis simultaneously, occupies the 2nd on the list of the preferential control of China's noxious chemical.Formaldehyde is a kind of important chemical material and organic solvent.Be usually used in making industrial goods such as resin, plastics, medicine, paint, synthon and various tamanoris, also can be used as sterilization, (Hesham R.Lotfy such as anticorrosion, Rashed I.G..A method for treating waste water containing form aldehyde[J] .Water Research, 2002,36:633-637).The main source of formaldehyde is Formaldehyde Production enterprise and is the waste water of the industry dischargings such as organic synthesis, wood working, dyestuff, system lacquer of raw material with formaldehyde in the environment.
The PARA FORMALDEHYDE PRILLS(91,95) wastewater treatment method mainly contains methods such as chemical reaction method (oxygenizements of pyrolusite catalyzed oxidation, clorox, Textone, dioxide peroxide, potassium permanganate etc.), electrochemical process, electrolytic oxidation, oxidation absorption method, physical adsorption techniques, ozone anion technology, nano photo catalyzed oxidation, non-thermal plasma trap and biological degradation at present.But the use repeatedly of chemical process can cause secondary pollution; Problems such as physical method ubiquity cost is higher, and complicated operation and treatment effect are undesirable.Relative, the microorganism rule is a material with the probiotics in the ecotope, cost is low, and is effective, and non-secondary pollution.Microorganism is a food with the organism in the pollutent, eutrophy element, and the raised growth breeding quickens to consume organism, pollutent, nutrition in the pollutent, pollutes thereby thoroughly administer.Elite microorganism can keep permanent activity; they are being " food " at the toxic substance that decomposes separately; a large amount of breedings fast; form the flora protective membrane on pollutent surface and shallow top layer; at any time cut the pollutent that permeates or volatilize from source of pollution extremely, thereby can work muchly because it can penetrate in the source of pollution, under the situation of not destroying starting materials; effectively thereby decomposing harmful substances takes stopgap measures and effects a permanent cure, and impedes a future disaster.Nontoxic, can harmful to human and cause secondary environmental pollution.
Mostly biological method commonly used at present is to use the Degradation Formaldehyde bacterium and the yeast of screening from all kinds of different habitats, and this quasi-microorganism is to poor environment, and not good as adaptability such as uv-radiation, low nutrition, low pH values, the breeding transmission capacity is relatively poor, and is difficult for preserving.Filamentous fungus is prevalent in water body and the edatope, and its mycelia physical efficiency provides bigger surface-area to contact with pollutent, helps the abundant degraded of pollutent; Its spore can adapt to all kinds of poor environments.Therefore, demand developing formaldehyde in a kind of effectively degrading waste water urgently, and safety, environmental protection, fungi strain efficiently.
Summary of the invention
An object of the present invention is to provide red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1.
Red silk ribbon attached to an official seal or a medal aspergillus provided by the present invention (Aspergillus nomius) SGFA1, its preserving number is CGMCC No.4538.
Another object of the present invention provides the application of described red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 in degradation of formaldehyde.
Another purpose of the present invention provides a kind of microbial inoculum of degradation of formaldehyde.
The microbial inoculum of degradation of formaldehyde provided by the present invention, its activeconstituents are described red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1.
The application of described microbial inoculum in degradation of formaldehyde also belongs to protection scope of the present invention.
Red silk ribbon attached to an official seal or a medal aspergillus provided by the present invention (Aspergillus nomius) SGFA1 can grow formaldehyde under aerobic condition as sole carbon source, simultaneously with its degraded.Under the pure culture condition, can be that the formaldehyde of 80mmol/L was degraded in 7 days fully with concentration.This bacterial strain is expected to play a significant role in the biological treatment of trade effluent.
Description of drawings
Fig. 1 is the upgrowth situation of red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 on rose bengal medium.
Fig. 2 is the conidiosporangium of red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1.
The variation of Fig. 3 formaldehyde content when red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 cultivated in the substratum that contains 80mmol/l formaldehyde.
Fig. 4 when red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 cultivated in the substratum that contains different concns formaldehyde mycelium dry weight variation.
The variation of Fig. 5 formaldehyde content when red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 cultivated in the substratum that contains different concns formaldehyde.
Fig. 6 when red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 cultivated in containing the substratum of different carbon sources mycelium dry weight variation.
The variation of Fig. 7 formaldehyde content when red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 cultivated in containing the substratum of different carbon sources.
Fig. 8 is the typical curve of the corresponding OD value of different concns formaldehyde standard substance.
Embodiment
Employed experimental technique is ordinary method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Separation and the evaluation of embodiment 1, red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1
One, the separation of red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1
The separation of red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 is divided into sampling and enrichment culture, screening and two steps of purifying, and concrete grammar is as follows:
1, sampling and enrichment culture
Get behind the Heilongjiang Province Furniture Factory untreated water outlet place mud sample enrichment culture immediately.On Bechtop, get 10g mud sample and place 90mL sterilized water, 180rmin
-1, place under 30 ℃ the condition concussion to cultivate 5 days.
2, screening and purifying
What screen usefulness is the PDA substratum that contains formaldehyde, and this culture medium preparation method is as follows:
Get the 200g potato, clean peeling is cut into small pieces, and adds water boil 30 minutes, and filtered through gauze adds the agar of 20g glucose and 2% again, is settled to 1000mL, 121 ℃ of sterilizations 20 minutes, and the cooling back adds 10mmol/L formaldehyde and stores standby.
The above-mentioned steps 1 concussion cultivation mould that growth is got up after 5 days is rule through repeatedly using the PDA culture medium flat plate that contains 10mmol/L formaldehyde, till separation obtains single bacterium colony.The mycelia top moved to receive on the PDA substratum repeat purifying 3 times continuously, both obtained the pure culture bacterial strain.
Two, the evaluation of red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1
The pure culture bacterial strain that above-mentioned experiment one is obtained carries out a series of morphological specificitys evaluations, and carries out DNA extraction, the amplification and the order-checking of ITS conserved regions sequence.This bacterial strain is growth in the form of a ring on rose bengal medium, the bacterium colony yellow-green colour, and reverse side eggshell yellow, thalline is flat thin, coarse, and inside is yellow, and outer most edge is a white, and the later stage is green slightly.Dry no transudate, surperficial radiationless shape line, matrix has irregular radiation fold rill (Fig. 1).The conidium fringe is cylindric, and is faint yellow.Conidiophore smoothly is with yellow.Conidiosporangium is spherical, and spore is botryoidalis (Fig. 2).
The compound method of rose bengal medium is as follows: with peptone 5g, glucose 10g, KH
2PO
41g, MgSO
47H
2After dissolving in O 0.5g and the agar 20g adding distilled water, add 1/3000 rose-bengal solution and (get rose-bengal 30 grams, 1000 milliliters of adding distil waters, be heated to whole dissolvings, promptly obtain 1/3000 rose-bengal solution) 100mL and paraxin 0.1g, be settled to 1000mL, packing, 121 ℃ of sterilization 20min are used for separating mould and yeast.
Utilize universal primer ITS1 and ITS4 to carry out the amplification of ITS conserved regions sequence, primer sequence is as follows:
ITS1：5′-TCCGTAGGTGAACCTGCGG-3′，
ITS4：5′-TCCTCCGCTTATTGATATGC-3′。
The pcr amplification condition is: 94 ℃ of 3min; 94 ℃ of 30s, 50 ℃ of 30s, 72 ℃ of 1.5min, 25 circulations; 72 ℃ of 10min.
Pcr amplification product is checked order, and sequencing result is shown in sequence in the sequence table 1.
With the red silk ribbon attached to an official seal or a medal aspergillus of a strain called after (Aspergillus nomius) SGFA1 in the pure culture bacterial strain of above-mentioned acquisition.
This red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center and (has been called for short CGMCC on 01 13rd, 2011, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101), preserving number is CGMCC No.4538.
The preparation method of the red silk ribbon attached to an official seal or a medal aspergillus of biological degradation effect detection (Aspergillus nomius) the SGFA1 first order seed of embodiment 2, red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 PARA FORMALDEHYDE PRILLS(91,95) is as follows:
Spore suspension that will PDA inclined-plane upper punch washes from the foregoing description 1 inserts in the PD substratum, and inoculum size is 2% (volume ratio), at 30 ℃, and 180rmin
-1, cultivated 4 days, promptly obtain primary seed solution.
The PD substratum: except that not adding the agar, all the other compositions are all identical with above-mentioned PDA substratum.
(1) the biological degradation effect detection of red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 PARA FORMALDEHYDE PRILLS(91,95)
With the primary seed solution that obtains is that 2% ratio is inoculated in the PD substratum that contains 80mmol/l formaldehyde and cultivates shaking speed 180rmin by volume
-1, 30 ℃ of temperature were cultivated 7 days.Simultaneously, establish the contrast of not inoculating primary seed solution.
Got the nutrient solution that adds bacterium processing and contrast respectively at the the the the the 1st, the 2nd, the 3rd, the 4th, the 5th, the 6th and the 7th day that inoculates, measure the formaldehyde residual content every day, measured altogether 7 days.3 repetitions, results averaged are established in experiment.The result adds in the nutrient solution that bacterium handles as shown in Figure 3, inoculate 7 days in concentration of formaldehyde on a declining curve, and the 7th day formaldehyde is all degraded; And the contrast nutrient solution in, inoculate 7 days in concentration of formaldehyde do not have obvious variation.This presentation of results, red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 degradable formaldehyde.
(2) red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 detects the degradation effect of different concns formaldehyde
With the primary seed solution that obtains is that 2% ratio is inoculated in respectively in the PD substratum that contains 30mmol/l, 50mmol/L and 80mmol/L formaldehyde and cultivates shaking speed 180rmin by volume
-1, 30 ℃ of temperature were cultivated 7 days.
Inoculation the 1st, the 2nd, the 3rd, the 4th, the 5th, the 6th with got different concentration of formaldehyde on the 7th day respectively and handle nutrient solutions, measure mycelia dry weight and formaldehyde residual content every day, measured altogether 7 days.3 repetitions, results averaged are established in experiment.The result as shown in Figure 4, i.e. upgrowth situation and the Degradation Formaldehyde situation of SGFA1 under 30mmol/L, 50mmol/L and 80mmol/L formaldehyde treated.As seen from the figure, SGFA1 is under the 30mmol/L formaldehyde treated, and mycelium dry weight reached 0.748g in the 7th day, and under the 50mmol/L formaldehyde treated, mycelium dry weight reached 0.418g in the 7th day, and under the 80mmol/L formaldehyde treated, mycelium dry weight reached 0.346g in the 7th day.This presentation of results concentration of formaldehyde is high more, and the same time of the bacteria growing of equal quality, upgrowth situation is poor more.
Inoculation the 1st, the 2nd, the 3rd, the 4th, the 5th, the 6th with got different concentration of formaldehyde on the 7th day respectively and handle nutrient solutions, measure the formaldehyde residual content every day, measured altogether 7 days.3 repetitions, results averaged are established in experiment.The result as shown in Figure 5.As seen from the figure, concentration of formaldehyde all significantly descends in the nutrient solution that different concentration of formaldehyde are handled, and 30mmol/L formaldehyde all can be degraded in SGFA13 days, 50mmol/L formaldehyde all can be degraded in 6 days, 80mmol/L formaldehyde all can be degraded in 7 days.
(3) substratum of different carbon sources processing is to the influence of red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 degradation of formaldehyde
Choose 4 kinds of carbon sources: glucose, lactose, sucrose, Zulkovsky starch, 2% ratio is added in the PD substratum by volume, the carbon source of 5 kinds of different treatment is set, be respectively: formaldehyde (handling 1), formaldehyde+2% glucose (handling 2), formaldehyde+2% lactose (handling 3), formaldehyde+2% sucrose (handling 4) and formaldehyde+2% Zulkovsky starch (handling 5), all the other compositions are identical.The culture medium preparation method of 5 kinds of different treatment is as follows:
(1) be that the culture medium preparation method of carbon source is as follows with formaldehyde: the 200g potato, clean peeling is cut into small pieces, and adds water boil 30 minutes, and filtered through gauze adds 2% agar again, is settled to 1000mL.Sterilized 20 minutes for 121 ℃, it is that the 80mmol/L storage is standby that the cooling back adds concentration of formaldehyde.
(2) be that the culture medium preparation method of carbon source is as follows with formaldehyde+2% glucose: the 200g potato, clean peeling is cut into small pieces, and adds water boil 30 minutes, and filtered through gauze adds the agar of 20g glucose and 2% again, is settled to 1000mL.Sterilized 20 minutes for 121 ℃, it is that the 80mmol/L storage is standby that the cooling back adds concentration of formaldehyde.
(3) be that the culture medium preparation method of carbon source is as follows with formaldehyde+2% lactose: the 200g potato, clean peeling is cut into small pieces, and adds water boil 30 minutes, and filtered through gauze adds the agar of 20g lactose and 2% again, is settled to 1000mL.Sterilized 20 minutes for 121 ℃, it is that the 80mmol/L storage is standby that the cooling back adds concentration of formaldehyde.
(4) be that the culture medium preparation method of carbon source is as follows with formaldehyde+2% sucrose: the 200g potato, clean peeling is cut into small pieces, and adds water boil 30 minutes, and filtered through gauze adds the agar of 20g sucrose and 2% again, is settled to 1000mL.Sterilized 20 minutes for 121 ℃, it is that the 80mmol/L storage is standby that the cooling back adds concentration of formaldehyde.
(5) be that the culture medium preparation method of carbon source is as follows with formaldehyde+2% Zulkovsky starch: the 200g potato, clean peeling is cut into small pieces, and adds water boil 30 minutes, and filtered through gauze adds the agar of 20g Zulkovsky starch and 2% again, is settled to 1000mL.Sterilized 20 minutes for 121 ℃, it is that the 80mmol/L storage is standby that the cooling back adds concentration of formaldehyde.
With red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 primary seed solution is that 2% ratio is inoculated in above-mentioned five kinds of substratum and cultivates by volume.Shaking speed 180rmin
-1, 30 ℃ of temperature were cultivated 7 days.
Got the different treatment nutrient solution respectively at the the the the the 1st, the 2nd, the 3rd, the 4th, the 5th, the 6th and the 7th day that inoculates, get 2 bottles every day and measure mycelia dry weights and formaldehyde residual content, measured altogether 7 days, 3 repetitions, results averaged are established in experiment.Result such as Fig. 6 and Fig. 7.
The result of Fig. 6 and Fig. 7 shows: red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 can utilize formaldehyde as the sole carbon source growth and breeding, 80mmol/L formaldehyde all can be degraded in 7 days.Simultaneously, this bacterium can be under the condition of different carbon sources metabolism formaldehyde.
The formaldehyde content measuring method is an acetylacetone method, and concrete grammar is: according to the formalin of different concns, in the ammonium acetate solution medium, generate yellow compound (3,5 one diacetyl-1,4 hydrogen lutidine) with the methyl ethyl diketone reacting by heating.This compound is to the selective absorption of visible light, and maximum to the visible absorption of wavelength 414nm, and its absorption value to visible light is directly proportional with formaldehyde solution.According to this principle, respectively the formaldehyde solution of several concentration known is carried out the measurement of absorbancy, draw the corresponding OD value of different concentration of formaldehyde typical curve, draw the graticule equation.
Concrete steps are as follows: get 6 10ml volumetric flask numberings, add 0 respectively, 2,4,6,8,10ml concentration is that (formaldehyde analytical pure solution is available from Chemical Reagent Co., Ltd., Sinopharm Group for the formaldehyde standard solution of 100mmol/l, catalog number is that CAS RN 50-00-0 concentration is 37%-40% (concentration of volume percent)), get this solution of 9.959ml and add the water constant volume to 1L), add water to 10ml and shake up (concentration of formaldehyde is shown in Table 1 in the volumetric flask of different numberings).1mL methyl ethyl diketone solution is added in the 4mL water, add the formaldehyde standard solution 1 μ l of different concns then, 65 ℃ of reaction 10min.When the solution cool to room temperature, measure its OD value.Adopt the 10mm cuvette, at wavelength 414nm place, with No. 0 be the reference measurement absorbancy, the OD pH-value determination pH the results are shown in Table 2.Draw the corresponding OD value typical curves (as shown in Figure 8) of different concentration of formaldehyde, draw the typical curve equation and be: y=0.0065x+0.0034 (R2=0.9998).
The formaldehyde standard solution of table 1 preparation different concns
The pairing OD value of formaldehyde standard substance concentration of different concns in table 2 typical curve
1mL methyl ethyl diketone solution is added in the 4mL water, add strain cultured solution 1 μ l then, 65 ℃ of reaction 10min.When the solution cool to room temperature, measure its OD value.Adopting the 10mm cuvette, at wavelength 414nm place, is the reference measurement absorbancy with water.According to the concentration of formaldehyde in the above-mentioned typical curve Equation for Calculating strain cultured solution that draws.
The compound method of methyl ethyl diketone solution is as follows: 50g ammonium acetate, 6mL glacial acetic acid and 0.5mL methyl ethyl diketone are dissolved in the 100mL water, promptly obtain methyl ethyl diketone solution.This solution can be stablized one month at least 4 ℃ of preservations.
The measuring method of mycelia dry weight is as follows: get the two-layer filtered through gauze of nutrient solution, collect thalline, thalline is placed on the quantitative analysis filter paper (filter paper is weighed in advance), dry to constant weight, weigh for 60 ℃.Mycelia dry weight=total mass-quantitative analysis filter paper weight.
Claims (4)
1. red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1, its preserving number is CGMCC No.4538.
2. the application of described red silk ribbon attached to an official seal or a medal aspergillus (Aspergillus nomius) SGFA1 of claim 1 in degradation of formaldehyde.
3. the microbial inoculum of a degradation of formaldehyde, its activeconstituents is the described red silk ribbon attached to an official seal or a medal aspergillus of claim 1 (Aspergillus nomius) SGFA1.
4. the application of the described microbial inoculum of claim 3 in degradation of formaldehyde.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111996123A (en) * | 2020-07-09 | 2020-11-27 | 武夷学院 | Endophytic fungus monascus sinensis and application thereof |
| CN112391294A (en) * | 2019-08-16 | 2021-02-23 | 广西壮族自治区林业科学研究院 | Aspergillus oryzae and application thereof in prevention and treatment of yellow-striped rice borers |
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| CN101250486A (en) * | 2008-04-14 | 2008-08-27 | 广东省生态环境与土壤研究所 | Use of aspergillus flavus H4 in degradation of formaldehyde |
| CN102021121A (en) * | 2010-08-13 | 2011-04-20 | 广东省生态环境与土壤研究所 | Halictidae aspergillus nomius strain and application thereof |
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| CN101250486A (en) * | 2008-04-14 | 2008-08-27 | 广东省生态环境与土壤研究所 | Use of aspergillus flavus H4 in degradation of formaldehyde |
| CN102021121A (en) * | 2010-08-13 | 2011-04-20 | 广东省生态环境与土壤研究所 | Halictidae aspergillus nomius strain and application thereof |
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| Title |
|---|
| 《FEMS Microbiology Letters》 20021231 Tetsuya K et al Purification and characterization of formate oxidase 137-142 1-4 第214卷, 第1期 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112391294A (en) * | 2019-08-16 | 2021-02-23 | 广西壮族自治区林业科学研究院 | Aspergillus oryzae and application thereof in prevention and treatment of yellow-striped rice borers |
| CN112391294B (en) * | 2019-08-16 | 2023-08-08 | 广西壮族自治区林业科学研究院 | Aspergillus peak and application thereof in Huang Yeming control |
| CN111996123A (en) * | 2020-07-09 | 2020-11-27 | 武夷学院 | Endophytic fungus monascus sinensis and application thereof |
| CN111996123B (en) * | 2020-07-09 | 2022-01-11 | 武夷学院 | Endophytic fungus monascus sinensis and application thereof |
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