CN102212975B - Separation method of pteroceltis tatarinowii fiber - Google Patents
Separation method of pteroceltis tatarinowii fiber Download PDFInfo
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- CN102212975B CN102212975B CN 201110086658 CN201110086658A CN102212975B CN 102212975 B CN102212975 B CN 102212975B CN 201110086658 CN201110086658 CN 201110086658 CN 201110086658 A CN201110086658 A CN 201110086658A CN 102212975 B CN102212975 B CN 102212975B
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Abstract
The invention relates to a separation method of a pteroceltis tatarinowii fiber, and the method comprises the following steps: placing 2-3-year-old pteroceltis tatarinowii stem bark into a pectase solution with the pH value of 4.5+/-0.5, and soaking at the temperature of 35+/-5 DEG C for 3+/-0.5 hours; and then flushing the pteroceltis tatarinowii stem bark after enzyme treatment with distilled water, then placing into 0.6% NaOH, cooking for 25+/-5 minutes at the temperature of 80-85 DEG C, taking out, and washing with the distilled water, so that the pteroceltis tatarinowii fiber can be separated into complete fiber cells. The pteroceltis tatarinowii stem bark processed by the separation method can be separated into the complete and clear fiber cells, thereby providing a high-quality raw material for papermaking, greatly reducing the using quantity of alkali by mass production, reducing the consumption of chemicals, not only saving the cost, but also reducing the environmental pollution.
Description
Technical field
The present invention relates to a kind of separation method of wingceltis fiber, belong to the biological cell separation technology field.
Background technology
String (plant fiber) is a kind of sclerenchyma that extensively is distributed in the seed plant, and its cell is elongated, and two ends are sharp-pointed, have thicker secondary wall, and simple pit is often arranged on the wall, does not generally have the protoplast of living when ripe.String mainly plays mechanical support effect in plant.In plant stem, like the herbaceous stem of ramie, hemp, flax and jute, the bast fibre bundle that tool is flourishing especially can be used to make various textiless; In the woody stem of some plants, the toughness fiber is also very flourishing, and they are good raw material of making specialties, like wingceltis, mulberry tree, paper mulberry etc.
The toughness fiber of wingceltis limb is a main raw material of making rice paper, is applicable raw materials with 2～3 years living sprays.Nitrate method (10% chromic acid and 10% nitric acid volume ratio are 1: the 1) (Gao Hui that adopts document to mention; Xu Bin; Shao Zhuoping. the chemical composition of wingceltis bark and cell wall structure [J]. economic forest research, 2007,25 (4): 28-33) separate 2～3 years living stem hide fibers of wingceltis; Microscopically is observed most of broken, the fracture of fiber, can't obtain complete fibrocyte.Nitrate method (10% chromic acid is 1: 1 with 10% nitric acid volume ratio) be commonly used to separate lumber fibre (Li Zhengli. plant tabletting technology [M]. Beijing: Science Press. 1978.), the wingceltis fiber is mainly bast fiber, so this method and inapplicable.
Tradition wingceltis leather slurry adopts alkaline process, but owing to the content of pigment, polysaccharose substance (like glue, plant mucilage, starch, pectic substance, many lactose) in the wingceltis skin is higher, the consumption of medicine in the time of can increasing soda pulping process.And adopt pectase solution-treated, available a spot of pectase to decompose a large amount of polysaccharose substances, thereby the consumption of chemicals when reducing soda pulping process alleviates the pollution to environment.
Summary of the invention
Problem to be solved by this invention provides a kind of method of wingceltis fiber separation, and the wingceltis fiber of handling through the present invention can intactly disperse, and is fit to produce in batches.
Technical scheme provided by the invention is: a kind of separation method of wingceltis fiber, 2～3 years living wingceltis stem skins are put into the pectase solution of pH 4.5 (± 0.5), and temperature 35 (± 5) ℃, soak time are 3 (± 0.5) hour; To pass through then wingceltis stem skin that enzyme handles with distilled water flushing after, place 0.6 % NaOH (0.6mg/100mL) under 80-85 ℃ of temperature, to boil 25 (± 5) min, take out with distilled water and clean, the wingceltis fiber is separable into complete fibrocyte.
The optimal concentration of above-mentioned pectase solution is 1594.6 (± 100) U/mg, and wherein pectase is with the hydrochloride buffer dissolving of pH 4.5 (± 0.5), and the two amount ratio is 10 (± 1) mg: 3 (± 1) mL.
That the wingceltis stem skin of handling through the present invention can separate into is complete, fibrocyte clearly, can be the raw material that papermaking provides high-quality, and the consumption of alkali when batch process can significantly reduce slurrying reduces the consumption of chemicals, has both practiced thrift cost, has alleviated the pollution to environment again.
Description of drawings
Fig. 1 is the embodiment of the invention 1The microphoto of the wingceltis fiber that processing obtains.
Fig. 2 is the embodiment of the invention 2The microphoto of the wingceltis fiber that processing obtains.
Fig. 3 is the embodiment of the invention 3The microphoto of the wingceltis fiber that processing obtains.
The specific embodiment
Preparation method is following:
1.The preparation of reagent
The preparation of pectase solution:
The hydrochloric acid solution mixing that 10 (± 1) mg pectase is added 3 (± 1) mL pH, 4.5 (± 0.5) obtains pectase solution.
The preparation of solution:
With 0.6 mg NaOH be dissolved in a small amount of distilled water and constant volume to 100 mL, obtain the NaOH dye liquor.
Draw materials: 2～3 years living wingceltis branches are cut into the long stub of 3 cm, and band girdle stem skin is cut into the stem skin small fragment of 2 mm * 20 mm again, and it is subsequent use to put into the refrigerator frozen coating.
. segregation: stem skin graft section is put into the pectase solution of the pH 4.5 (± 0.5) for preparing, and temperature remains on 35 (± 5) ℃, and soak time is 3 (± 0.5) hour.
Flushing and heat are boiled: the stem skin graft section after enzyme is handled is used distilled water flushing, places 0.6% NaOH solution then, keeps 80-85 ℃ of temperature, and heat is boiled 25 (± 5) min; Taking-up washes down with distilled water.
Microscopy: the wingceltis fiber after will handling amplifies 100 times at microscopically and carries out microscopy after steps such as centrifugal, fixing, dyeing, can detect and separate complete fibrocyte.
Embodiment 1:2 years living wingceltis stem skins are cut into 2 mm * 20 mm small pieces, get 5 pectase solution of putting into pH 4.0, the pectase solution concentration is 1494.6 U/mg, and 30 ℃ of temperature, soak time are 2.5 hours; Stem skin graft section after then enzyme being handled is placed in the 0.6% NaOH solution with distilled water flushing, keeps 80-85 ℃ of temperature, and heat is boiled 25 min; Taking-up washes down with distilled water, puts into clean penicillin bottle, adds 2 mL distilled water,, pours in the 10 mL centrifuge tubes to from broken with thick glass rod concora crush, and centrifugal 5 min of 3500 r/min carefully abandon supernatant, and deposition is dashed with 1 mL distilled water and dissolved; Get 1 with dropper behind the mixing, coat on the slide, dry fixing on alcolhol burner; The sarranine of dripping 10 % then 10 min that dye are with microscopy behind the distilled water flushing.Repeat 5 times, promptly get 5 detections altogether.100 times of microscopy multiplication factors, microscopically (referring to Fig. 1) observed result show that the wingceltis stem hide fiber after the present invention handles is separable to be complete fibrocyte.This sample detects 101 of fibrocytes altogether, average length 2.641 mm, mean breadth 14.63um.Fig. 1 is this sample part fibrocyte.
Embodiment 2:2 years living wingceltis stem skins are cut into 2 mm * 20 mm small pieces, get 5 pectase solution of putting into pH 4.5, the pectase solution concentration is 1594.6 U/mg, and 35 ℃ of temperature, soak time are 3 hours; Stem skin graft section after then enzyme being handled is placed in the 0.6% NaOH solution with distilled water flushing, keeps 80-85 ℃ of temperature, and heat is boiled 25 min; Taking-up washes down with distilled water, puts into clean penicillin bottle, adds 2 mL distilled water,, pours in the 10 mL centrifuge tubes to from broken with thick glass rod concora crush, and centrifugal 5 min of 3500 r/min carefully abandon supernatant, and deposition is dashed with 1 mL distilled water and dissolved; Get 1 with dropper behind the mixing, coat on the slide, dry fixing on alcolhol burner; The sarranine of dripping 10 % then 10 min that dye are with microscopy behind the distilled water flushing.Repeat 5 times, promptly get 5 detections altogether.100 times of microscopy multiplication factors, microscopically (referring to Fig. 2) observed result show that the wingceltis stem hide fiber after the present invention handles is separable to be complete fibrocyte.This sample is total to 107 in detection fibers cell, average length 2.682mm, mean breadth 15.12um.Fig. 2 is this sample part fibrocyte.
Embodiment 3:3 years living wingceltis stem skins are cut into 2 mm * 20 mm small pieces, get 5 pectase solution of putting into pH 5.0, the pectase solution concentration is 1694.6 U/mg, and 40 ℃ of temperature, soak time are 3.5 hours; Stem skin graft section after then enzyme being handled is placed in the 0.6% NaOH solution with distilled water flushing, keeps 80-85 ℃ of temperature, and heat is boiled 25 min; Taking-up washes down with distilled water, puts into clean penicillin bottle, adds 2 mL distilled water,, pours in the 10 mL centrifuge tubes to from broken with thick glass rod concora crush, and centrifugal 5 min of 3500 r/min carefully abandon supernatant, and deposition is dashed with 1 mL distilled water and dissolved; Get 1 with dropper behind the mixing, coat on the slide, dry fixing on alcolhol burner; The sarranine of dripping 10 % then 10 min that dye are with microscopy behind the distilled water flushing.Repeat 5 times, promptly get 5 detections altogether.100 times of microscopy multiplication factors, microscopically (referring to Fig. 3) observed result show that the wingceltis stem hide fiber after the present invention handles is separable to be complete fibrocyte.This sample is total to 119 in detection fibers cell, average length 2.653mm, mean breadth 15um.Fig. 3 is this sample part fibrocyte.
Claims (1)
1. the separation method of a wingceltis fiber is put into the pectase solution of pH 4.5 ± 0.5 with 2～3 years living wingceltis stem skin graft sections, and 35 ± 5 ℃ of temperature, soak time are 3 ± 0.5 hours; To pass through then wingceltis stem skin that enzyme handles with distilled water flushing after, placing concentration is that per hundred milliliters 0.6 milligram NaOH boils 25 ± 5min under 80-85 ℃ of temperature, takes out with distilled water and cleans, wingceltis stem hide fiber is separable to be complete fibrocyte; The concentration of pectase solution is 1594.6 ± 100 U/mg, and wherein pectase is with the hydrochloride buffer dissolving of pH 4.5 ± 0.5, and the two amount ratio is 9～11mg: 2～4mL.
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| CN102823403B (en) * | 2012-08-31 | 2014-08-13 | 泰安市泰山林业科学研究院 | Breeding method of pteroceltis tatarinowii polyploid |
| CN113445365B (en) * | 2020-03-26 | 2023-12-12 | 杭州特种纸业有限公司 | Quantitative filter paper and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4568739A (en) * | 1983-11-22 | 1986-02-04 | Helmic, Inc. | Method for degumming decorticated plant bast fiber |
| CN1522323A (en) * | 2001-06-29 | 2004-08-18 | 诺维信北美公司 | Single-bath preparation of cellulosic materials |
| CN101463503A (en) * | 2009-01-15 | 2009-06-24 | 安徽农业大学 | Ramie compositional biological enzyme degumming technological process |
| CN101638811A (en) * | 2009-08-31 | 2010-02-03 | 盐城纺织职业技术学院 | Degumming technology of mulberry bark |
| CN101942702A (en) * | 2010-09-10 | 2011-01-12 | 中国科学院过程工程研究所 | Method for preparing coconut shell fibers by removing pectin from coconut shell through steam explosion |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4568739A (en) * | 1983-11-22 | 1986-02-04 | Helmic, Inc. | Method for degumming decorticated plant bast fiber |
| CN1522323A (en) * | 2001-06-29 | 2004-08-18 | 诺维信北美公司 | Single-bath preparation of cellulosic materials |
| CN101463503A (en) * | 2009-01-15 | 2009-06-24 | 安徽农业大学 | Ramie compositional biological enzyme degumming technological process |
| CN101638811A (en) * | 2009-08-31 | 2010-02-03 | 盐城纺织职业技术学院 | Degumming technology of mulberry bark |
| CN101942702A (en) * | 2010-09-10 | 2011-01-12 | 中国科学院过程工程研究所 | Method for preparing coconut shell fibers by removing pectin from coconut shell through steam explosion |
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