CN102179011B - Infrared-ultraviolet composite therapeutic apparatus - Google Patents

Infrared-ultraviolet composite therapeutic apparatus Download PDF

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CN102179011B
CN102179011B CN 201110051842 CN201110051842A CN102179011B CN 102179011 B CN102179011 B CN 102179011B CN 201110051842 CN201110051842 CN 201110051842 CN 201110051842 A CN201110051842 A CN 201110051842A CN 102179011 B CN102179011 B CN 102179011B
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infrared
treatment
control system
ultraviolet
vitiligo
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CN102179011A (en
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赵广
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赵广
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Abstract

本发明是一种用红外线和紫外线共同作用治疗皮肤病的医疗设备,由红外线、紫外线双光源、灯罩、可活动支撑杆、电源和控制台(包括双开关控制系统、温控系统及时间控制系统)组成。 The present invention is a method of treating dermatological interaction with infrared and ultraviolet medical devices by infrared, ultraviolet double light, shade, a movable rod, and console power (including dual switch control system, temperature control system and time control system )composition. 该设备可以照射全身各个部位的皮肤,能有效治疗白癜风等多种皮肤疾病,具有操作简便、安全、可靠、疗效显著,易于被患者接受等优点。 The apparatus may be irradiated skin all parts of the body, can effectively treat a variety of skin diseases such as vitiligo, is simple, safe, reliable, efficacious, easily accepted by patients and the like.

Description

红外线-中波紫外线治疗仪 Infrared - ultraviolet B therapy device

技术领域 FIELD

[0001] 本发明涉及医疗设备,特别是一种用红外线和紫外线共同作用于人体皮肤以治疗白癜风为主的多种皮肤病的光辐射治疗仪。 [0001] The present invention relates to medical equipment, in particular a infrared and ultraviolet interaction with human skin to treat a variety of skin vitiligo based optical radiation treatment instrument.

背景技术[0002] 人类皮肤的颜色主要与表皮中黑素细胞(melanocytes,MC)合成黑素以及黑素传递至周围角质形成细胞(keratinocytes, KC)有关。 [0002] The main color of the human skin epidermis transmission and melanocytes (melanocytes, MC) and melanin synthesis melanin to the surrounding keratinocytes (keratinocytes, KC) relevant. 在日光福射的影响下,皮肤细胞会通过自身复杂的调控体系减少/避免日光辐射可能对细胞造成的损伤,其中黑素颗粒扮演着类似超氧化物歧化酶的作用。 Under the influence of sunlight blessing shoot, skin cells will pass their own complex regulatory systems to reduce / avoid damage to cells caused by solar radiation may, where melanin granules play a similar role of superoxide dismutase. 黑素颗粒通过吸收、分散氧化应激产物、清除自由基,阻止细胞DNA、蛋白和膜脂质的损伤,进而起到重要的皮肤防御和保护作用。 Melanin granules by absorbing, distributing products of oxidative stress, free radical scavenging, the DNA to prevent cells, proteins and damage of membrane lipids, and thus plays an important role in skin defense and protection. 皮肤暴露于日光下,可引起皮肤晒黑、光老化和光癌变。 Skin is exposed to sunlight can cause sunburn, photoaging and cancer light. 既往的研究认为这些改变主要与紫外线(ultraviolet,UV),尤其是中波紫外线(ultraviolet B, UVB)有关。 Previous studies believe that these changes mainly with UV (ultraviolet, UV), especially UVB (ultraviolet B, UVB) related. 但近来,越来越多的证据表明日光中主要的能量物质-热(红外线)对皮肤细胞也具有类似UV的作用,同时热(红外线)还可增加皮肤细胞对UV的耐受性。 But recently, more and more evidence that sunlight major energy matter - thermal (infrared) on skin cells also have a role similar to UV, as well as heat (infrared) can also increase the skin cells to UV resistance. 虽然目前人们已经在UV辐射对MC生理及病理功能的影响方面做了大量的研究,但热(红外线)对MC生理功能影响的研究却刚刚起步,这为我们在热疗治疗色素性皮肤病方面提供了一个广阔的空间。 Although studies have been done a lot in the current impact of UV radiation on MC physiological and pathological function of aspects, but the thermal (infrared) study the physiological function of the impact on the MC has just started, which for us in terms of hyperthermia treatment of pigmented skin disease It provides a broad space. 我们知道皮肤是人体保护内部器官不受外界环境损伤(如日光、射线、机械刺激等)的重要屏障。 We know that skin is the body's internal organs to protect from environmental damage (such as sunlight, radiation, mechanical stimulation, etc.) is an important barrier. 急性日光暴露会造成光晒伤和光晒黑;日光长期照射会引起皮肤细胞的光损伤,产生光老化和光癌变。 Acute exposure to sunlight can cause sunburn and light light tan; prolonged exposure to sunlight can cause light damage skin cells, to produce photo-aging and cancer light. 无论短期或长期接受日光辐射,皮肤最明显的变化就是照射部位形成红斑和色素沉着。 Whether short or long term to solar radiation, the most obvious changes in the skin is irradiated sites form erythema and pigmentation. 研究发现,黑素细胞产生的黑素颗粒是人体对日光天然的防护剂。 The study found that melanin granules of melanin cells are the body's natural protective agent against the sun. 黑素颗粒通过吸收日光、分散氧化应激产物、清除自由基,从而阻止日光引起的细胞DNA、蛋白和膜脂质的损伤,进而起到重要的皮肤防御作用。 Melanin granules by absorbing sunlight, dispersion products of oxidative stress, scavenging free radicals, thereby preventing the cell the DNA, membrane lipids and protein damage caused by sunlight, and thus play an important role in skin defense. 色素沉着就是皮肤对日光辐射等环境刺激直接进行防御的结果。 Pigmentation is the skin of solar radiation and other environmental stimuli direct result of defense. 日光通常包括UV(290-400nm)、可见光(400_760nm)和红外线(760nm-lmm)三部分。 Generally it includes three parts daylight UV (290-400nm), visible (400_760nm) and (760nm-lmm) infrared. 既往的研究认为,UV辐射尤其UVB是日光照射引起皮肤诸多效应的主要物质。 Previous studies that, UV radiation, especially sunlight UVB is the main cause of many substances skin effect. 过去几年,大量的日光影响皮肤功能的研究也主要是围绕UV来展开的。 Over the past few years, a large number of studies of the sun affects the skin function is mainly to expand around the UV. 但最近,学者们逐渐认识到占日光总能量54. 3%的红外线(主要是其产生的热效应)对皮肤也有类似UV的作用,同时热对UV辐射引起的皮肤损伤有积极的保护作用。 But recently, researchers have come to realize accounted for daylight total energy 54.3% of infrared (mainly thermal effects it produces) on the skin has a similar UV role, but the heat of UV skin damage caused by radiation have a positive protective effect.

[0003] 很早以前人们就发现一些长期烤火或围坐于火炉旁的人会得火激红斑,临床表现为暴露于热的部位出现网状色素沉着、毛细血管扩张等改变。 [0003] long ago people discovered that some of the long-term warming himself sitting on the stove or jump on the fire will get excited erythema, clinical manifestations of exposure to heat parts of the network appear pigmentation, telangiectasia and other changes. 这些改变同UV引起的皮肤光老化类似。 These changes induced photoaging of the skin with similar UV. 同UV—样,慢性热暴露也会直接引起表皮增厚、色素增加、真皮弹力纤维组织损伤,甚至引起皮肤细胞癌变。 UV- same kind, chronic heat exposure can also cause direct epidermal thickening, increased pigmentation, dermal elastic fibers tissue damage, or even cause skin cancer cells. 越来越多的研究证据表明热(红外线)暴露会直接损伤细胞和细胞器膜,引起DNA损伤,从而直接引起皮肤黑化、老化和癌变,并可加强UV引起的皮肤晒黑、光老化和光致癌性效应。 More and more evidence shows that heat (infrared) directly exposed to damage cells and cell membrane, causing DNA damage, thereby directly causing blackening of the skin, aging and cancer, and to strengthen the UV-induced tanning, photoaging and photocarcinogenesis effect. 除此之外,热(红外线)还具有增加皮肤细胞对UV耐受性的作用。 In addition, thermal (infrared) also has the effect of increase in skin cells to UV tolerance. 研究发现当UVB照射皮肤后立即浸入40°C水中比立即浸入30°C水中其红斑潜伏期会缩短。 UVB irradiation was found when skin immediately immersed in 40 ° C water than immediately immersed in 30 ° C water which will shorten the latency erythema. 但其诱导的红斑效应会比30°C水中的减弱;Menezes和Danno等也分别证实红外线可明显抑制UVB辐射诱导的皮肤KC凋亡和成纤维细胞的死亡。 But its effect induced erythema than 30 ° C reduced the water; Menezes, and the like were also confirmed by infrared Danno significantly inhibited UVB radiation-induced apoptosis and KC skin fibroblasts to death. Boreham等在应用热浴(40°C水浴,每周2次,8周I疗程)治疗银屑病的过程中发现,热浴明显增加了皮肤对日光辐射的耐受性和晒黑反应。 Boreham, etc. In applying a hot bath (40 ° C water bath, 2 times a week, 8 weeks treatment I) found in the treatment of psoriasis, heating bath and significantly increases the resistance to solar radiation and tanning of skin reactions.

[0004] 申请者在多年的白癜风治疗中也体会到,中药热敷(45°C,30min,I次/日)可明显提高白癜风的复色率。 [0004] The applicants also many years of experience to the treatment of vitiligo, Chinese hot (45 ° C, 30min, I times / day) can significantly improve the repigmentation of vitiligo. 本课题组近期的研究结果也初步证实热应激(> 39°C,作用MC60min)有直接的促黑素合成作用,同空白对照组相比有统计学差异(P < O. 05),该作用在42 V达最大。 The results also our group recently confirmed by a preliminary heat stress (> 39 ° C, the role MC60min) has a direct role in stimulating melanin synthesis, a significant difference (P <O. 05) compared with the control group, the role in the 42 V up to the maximum. 上述研究均提示我们热可能同UV —样对皮肤色素系统具有重要的调节作用,二者之间可能存在一定的协同和保护效应。 These studies strongly suggest that we may heat with UV - has an important role in the regulation of the kind of skin pigment system, there may be a synergistic effect between the two and protection. 基于此,申请者在2008年全国皮肤性病专业委员会中西医结合色素病学组《白癜风诊疗指南》修订会上提出将热疗作为一种新的白癜风治疗方法,该提议得到了与会专家的一致认可。 Based on this, the applicant Integrative pigment disease study group "vitiligo treatment guidelines" proposed amendments at the meeting hyperthermia as a new treatment for vitiligo skin in the 2008 National Professional Committee of sexually transmitted diseases, the proposal was endorsed unanimously by the participating experts . 目前为止,人们就UV对MC生理功能的影响已经做了大量的实验研究,但对热和MC生理功能的关系却知之甚少。 So far, people have UV Experimental study of the physiological function of the MC have done a lot, but the relationship between heat and MC physiological functions are poorly understood. 通过文献检索,我们发现国外仅有两篇相关报道且结果也不尽相同,国内则尚未发现有类似的研究。 Literature search, we found only two relevant reports and the results are not the same foreign and domestic have not yet been found to have similar research. 1998年Nakazawa等首先比较了人表皮MC对两种主要的外源性刺激UVB和热的细胞反应,结果发现:热(42°C,60min)和UVB (20mJ/cm2)辐射处理纯化培养人表皮MC 3d后,MC树突增多延长、胞体增大,细胞酪氨酸酶活性和黑素合成增加、增殖率下降,但6天后恢复到正常对照组水平;热和UVB均促进了KC-MC共培养体系和组织工程皮肤中有功能活性的MC数量增加。 In 1998 Nakazawa et al compared the first of the two major epidermal MC exogenous stimuli UVB and heat the reaction cell, found: hot (42 ° C, 60min) and UVB (20mJ / cm2) irradiation treatment was purified cultured human epidermal after the MC 3d, MC increased dendritic extension, increasing the cell body, cell tyrosinase activity and melanin synthesis, decreased proliferation rate, but after 6 days to restore the normal control level; promotes both thermal and UVB KC-MC co training system and tissue-engineered skin in an increase in the number of MC functional activity.

[0005]目前我们通过实验证明单独使用红外线或者紫外线照射体外培养的黑素细胞均能使细胞的酪氨酸酶活性和黑素含量增加,如果联合应用两种光源先后照射体外培养的黑素细胞后,不但黑素细胞的酪氨酸酶活性和黑素含量明显高于两种光源单独照射,而且红外线的热效应还能有效地保护紫外线对黑素细胞的破坏作用。 [0005] We are currently using infrared rays or ultraviolet irradiation solely by in vitro experiments demonstrated both melanocytes and tyrosinase activity can increase melanin content of the cells, if the combination of two sources successively irradiated melanocytes in vitro after only tyrosinase activity and melanin content of melanocytes alone was significantly higher than two kinds of light source, but infrared thermal effect but also effectively protect the damaging effects of ultraviolet radiation on melanocytes.

[0006] 白癜风虽然发病机制尚不清楚,但其主要表现为皮肤色素脱失或者减退为特征的皮肤科常见疾病,组织病理检查证实白癜风患者皮损基底层黑素细胞减少或者消失,这正是白癜风皮损形成的病理基础,所以我们在治疗白癜风时的主要原则就是减轻皮损区黑素细胞的破坏并且增强皮损区残存黑素细胞的活性,使其增殖、分化及迁移到色素脱失的部位,从而达到使白癜风复色的效果。 [0006] Although the pathogenesis of vitiligo is unknown, but its mainly for skin depigmentation or loss of dermatology common disease characterized by histopathological examination confirmed vitiligo lesions disappear or decrease basal layer melanocytes, which is formation of lesions of pathological basis vitiligo, we main principle in the treatment of vitiligo is to reduce the damage of melanocytes lesional and lesional enhancing activity remaining melanocytes, so as proliferation, differentiation, and migration to the depigmentation portions, so as to achieve the effect of complex color vitiligo. 虽然白癜风的真正病因还不清楚但是目前被学术界所认可的几种相关原因有:自身免疫紊乱、氧化应激、遗传学说等等,所以我们治疗白癜风一般都采用药物治疗、物理治疗、以及手术治疗多种方法联合治疗的方式。 Although the actual cause of vitiligo is unknown but there are several related causes it has been recognized by the academic community: autoimmune disorders, oxidative stress, genetics and so on, so we generally use the treatment of vitiligo medication, physical therapy, and surgery Combination therapy methods to treat a variety of ways. 而现在临床上使用的治疗白癜风得物理治疗仪包括窄普中波紫外线(NB-UVB)、308nm准分子激光、长波紫外线(UVA)、低能氦氖激光等类型,尚未见到应用红外线以及红外线和紫外线共同治疗白癜风的设备。 Now used clinically Vitiligo The physical therapy apparatus P includes a narrow band ultraviolet (NB-UVB), 308nm excimer laser, ultraviolet (of UVA), and other types of low-energy helium-neon laser, has not been seen by infrared and infrared and UV treatment of vitiligo common equipment.

[0007] NB-UVB能通过刺激角质形成细胞释放碱性成纤维细胞生长因子(bFGF)和内皮素-I (ET-I)来促进黑素细胞增殖,同时它还能提高黑素细胞自身的基质金属蛋白酶-2(MMP-2)和局部黏着斑激酶(FAK)的活性而加速了黑素细胞的迁移,以达到使白癜风皮损复色的目的。 [0007] NB-UVB keratinocytes by stimulating the release of basic fibroblast growth factor (bFGF) and endothelin -I (ET-I) to promote melanocyte proliferation, and it can improve the melanocytes themselves matrix metalloproteinase -2 (MMP-2) and focal adhesion kinase activity (of FAK) is accelerated migration of melanocytes, vitiligo lesions to achieve complex color object. 另外,NB-UVB还能使皮肤局部T淋巴细胞DNA形成胸腺嘧啶二聚体,诱导T淋巴细胞凋亡,从而抑制皮肤局部的免疫应答也是治疗白癜风的作用机制之一。 Further, NB-UVB can cause local skin T lymphocyte DNA Dimer formation, induce apoptosis of T lymphocytes to thereby suppress immune response is a local skin treatment vitiligo one of the mechanisms. 308nm准分子激光是一种氯化氙(XeCl)准分子激光,其波长在UVB范围内。 308nm is a xenon chloride excimer laser (an XeCl) excimer laser having a wavelength in the UVB range. 研究发现308nm准分子激光能有效地诱导皮损局部浸润的T淋巴细胞凋亡,其诱导T淋巴细胞凋亡的作用甚至优于NB-UVB。 308nm excimer laser was found to effectively induce lesions infiltration and apoptosis of T lymphocytes, which induced apoptosis of T lymphocytes even better than NB-UVB. UVA是临床上应用最早的治疗白癜风的物理疗法,其治疗白癜风的机制与其他光疗的机制基本相同,主要是通过抑制皮损局部的细胞免疫应答并为黑素细胞提供一个有利的增殖微环境而达到一个治疗白癜风的目的,UVA在治疗白癜风领域应用已经了将近一个世纪,其疗效是值得肯定的,但是其副作用过多,所以在一定程度上它的位置已经逐渐被NB-UVB和308nm准分子激光所替代。 UVA is the Physical Therapy application of the first treatment of vitiligo clinical mechanism mechanism other phototherapy treatment of vitiligo is substantially the same, mainly response by inhibiting the local cellular immune lesions and provide a favorable proliferation microenvironment of melanocytes achieve a treatment of vitiligo, UVA has applications in the treatment of vitiligo almost a century, and its effect is worthy of recognition, but its side effects too, so to some extent, its position has been gradually NB-UVB and 308nm excimer laser replaced. 低能氦氖激光治疗白癜风还没有被广泛应用于临床,但有学者在这方面做了一些尝试性研究发现用低能氦氖激光3. OJ/cm2,每周I〜2次治疗40例稳定性头颈部节段型白癜风,平均治疗17次后,皮损开始出现复色,有60%的患者经过连续治疗后复色面积大于50%。 Low-energy helium-neon laser treatment of vitiligo has not been widely used clinically, but some scholars have made attempts in this regard a number of studies found that low-energy helium-neon laser 3. OJ / cm2, I~2 times a week treatment of 40 cases of head stability neck segmental vitiligo, after an average of 17 times treatment, lesions began to re-color, 60% of patients after continuous treatment area greater than 50% repigmentation. 此外该学者还比较了治疗前后皮损部位的血流变化和肾上腺素受体的应答情况后认为低能氦氖激光可能是通过修复功能异常的交感神经来达到治疗白癜风的目的。 After the addition, the researchers also compared the responsiveness to changes in blood flow and skin lesions adrenergic receptors before and after treatment considered low-energy helium-neon laser may be sympathetic to achieve the purpose of treatment of vitiligo by abnormal repair.

[0008] 虽然以上治疗设备的疗效尚可,但是其治疗时出现的副作用仍不能小觑,有时会对患者的身体造成危害,不利于患者疾病的康复。 [0008] While the above therapeutic efficacy of the device is acceptable, but the side effects of their treatment can not be underestimated, and sometimes will cause harm to the patient's body, is not conducive to rehabilitation of patients with the disease. 另外现在临床应用的这些治疗设备都是大型化设备工作功率大,剂量控制要求高,需要专业技术人员操作,不能用于家庭使用,患者必须定期到医院接受治疗,非常不便致使患者依从性不高,从而影响治疗效果。 Also now these therapeutic devices are large-scale clinical application of large operating power equipment, high-dose control requirements, the need of professional and technical personnel to operate, not for home use, patients must regularly to the hospital for treatment, causing great inconvenience to patient compliance is not high , thus affecting the therapeutic effect. 此外,我们还没有见到在临床上单独使用红外线治疗白癜风的设备,所以本发明不但是对目前临床其他治疗仪器的一个改进,更是为白癜风的治疗提供一种新的治疗理念。 In addition, we have not seen the treatment of vitiligo infrared devices used alone in clinical practice, the present invention is not only to present an improved clinical treatment of other instruments, but also provide a new therapeutic concept for the treatment of vitiligo.

发明内容 SUMMARY

[0009] 本发明的目的在于弥补单一紫外线在治疗白癜风方面的不足,应用红外线(主要是热效应)和紫外线促进黑素细胞活性的协同作用来提高白癜风患者的皮损治疗的复色效果,同时利用热效应能明显降低紫外线对黑素细胞的破坏,从而降低原有设备治疗的副作用,另外该设备被设计成为一种医疗型及便携式家用型治疗仪,方便了患者使用,从而提高了患者治疗的依从性。 [0009] The object of the present invention is to make up a single UV insufficient application of infrared vitiligo areas (mainly thermal effects) to improve the complex color effects of lesions treated patients with vitiligo and UV synergistic cell activity melanin promotion, while using can significantly reduce the thermal effect on melanocytes UV damage, thereby reducing the side effects of existing treatment devices, the additional medical device is designed as a portable type and a domestic type of treatment, the patients convenience, thereby improving the patients compliance sex.

[0010] 本发明的目的是通过以下技术方案来实现的: [0010] The object of the present invention is achieved by the following technical solution:

[0011] 本治疗仪包括红外线、紫外线双光源、灯罩、可活动的支撑杆和控制台。 [0011] The present treatment device includes infrared, ultraviolet double light, shade, and the movable support bar console. 双光源分别由两个相同的红外线和紫外线灯管发出,可活动支撑杆有两根可活动金属杆构成(似台灯的支撑杆),控制台设置有双光源开关各一个,红外线温度控制旋钮一个,红外线和紫外线辐照时间设定旋钮各一个。 Dual light source composed of two identical emit infrared and ultraviolet lamps, two movable support rod movable metallic rod (support rod-like lamp), the console is provided with a double light switch each, an infrared temperature control knob , infrared and ultraviolet radiation each time a setting knob.

[0012] 其中:安装发光灯管的灯罩能绕着灯管的纵轴作360°旋转,方便患者照射身体的不同侧面皮损,同时灯罩还可绕着灯管的横轴作180°旋转,另外可活动支撑杆可以上下调节光源的高度,这种从三种不同维度上调节光源位置的设计可以满足对任何部位皮损的治疗需要。 [0012] wherein: the light emitting lamp shade mounted about a longitudinal axis of the lamp can be rotated through 360 °, to facilitate different aspects of the patient's body is irradiated skin lesions, while the horizontal axis around the lamp shade may be rotated by 180 °, Further support rod movable up and down to adjust the height of the light source, adjusting the light source position from which the three different dimensions are designed to meet the need for any treatment site lesions.

[0013] 本发明的控制系统采用分离控制的方式,即红外光源和紫外光源的开关分离,红外光源和紫外光源的辐照时间控制系统分离,这种分离控制方式有利于两种光源进行序贯的辐照治疗。 [0013] The control system of the invention by way of separate control, i.e., an infrared light source and a switch separate UV light source, an infrared light source and the irradiation time of the ultraviolet light source separated from the control system, which facilitate separation control two light sources for sequential the radiation treatment. 另外本发明加用了红外线温度控制系统,从而使红外线的热效应不会过高而引起皮肤烫伤或者过低而达不到治疗目的。 Also with the present invention incorporating infrared temperature control system, so that the thermal effect of infrared rays is not too high or too low and cause skin burns and reach therapeutic purposes.

附图说明 BRIEF DESCRIPTION

[0014] 图I :体外培养纯化的黑素细胞 [0014] FIG. I: Purification vitro melanocyte

[0015] 图2 :黑素细胞的Masson-Fontana染色鉴定 [0015] FIG. 2: Masson-Fontana staining melanocytes

[0016] 图3 :不同温度的热应激对体外培养的正常人黑素细胞增殖率的影响[0017] 图4 :不同剂量的UVB辐照对体外培养的正常人黑素细胞增殖率的影响 [0016] Figure 3: Effect of different temperatures of heat stress on the proliferation rate of normal human melanocytes in vitro [0017] Figure 4: Effect of different doses of UVB irradiation on the proliferation rate of normal human melanocytes in vitro

[0018] 图5 :不同条件对黑素细胞酪氨酸酶活性和黑素含量的影响 [0018] Figure 5: Effect of different conditions on tyrosinase activity and melanin content of melanocytes

[0019] 注:*与正常对照、UVB以及热处理组UVB或热处理组酪氨酸酶活性相比有统计学差异P < O. 05 ·'#与正常对照、UVB以及热处理组黑素量相比有统计学差异P < O. 05。 [0019] Note: * and normal controls, UVB and UVB heat treated or heat treated tyrosinase activity compared with a statistically significant difference compared to P <O. 05 · '# normal control, UVB, and heat treated amount of melanin there were significant differences P <O. 05.

[0020] 图6 :本发明的外观结构示意图。 [0020] FIG 6: is a schematic view of an external configuration of the present invention. (附图中数字指代:1、红外光源开关;2、紫外光源开关;3、红外线温控旋钮;4、红外光辐照时间设定装置;5、紫外光辐照时间设定装置;6、灯罩;7、红外光源灯管;8、紫外光源灯管;9、控制台;10、可活动的支撑杆;11、电源) (Numbers in the drawings refer to: 1, an infrared light switch; 2, the ultraviolet light source switch; 3, infrared temperature control knob; 4, infrared light irradiation time setting means; 5, UV irradiation time setting means; 6 , shade; 7, an infrared light source lamp; 8, the ultraviolet light source lamp; 9, console; 10, the movable support bar; 11, power supply)

[0021] 图7:本发明的原理示意图[0022] 图8 :双光源的平面示意图 [0021] Figure 7: a schematic view of the principles of the present invention [0022] FIG. 8: a schematic view of a plane light source bis

[0023] 图9 :控制台示意图 [0023] Figure 9: a schematic view console

具体实施方式 Detailed ways

[0024] 下面结合附图对本发明作进一步详述。 [0024] The following drawings in conjunction with the present invention will be further described in detail.

[0025] 首先介绍一下我们如何通过实验证实红外线的热效应和紫外线对黑素细胞的协同促进作用。 [0025] First, tell us about how we experimentally confirmed that infrared thermal effects and the synergistic effects of ultraviolet radiation on melanocytes.

[0026] I、正常人黑素细胞的培养和传代:将正常人包皮修剪成全厚皮,用5g/L的Dispase酶消化后,分离真皮与表皮,表皮用2. 5g/L胰蛋白酶消化液消化并制成单细胞悬液,置黑素细胞培养液(含5%的FBS,100U/ml青霉素和100ug/ml链霉素,bFGF的CDMB153培养基)中,在37°C、5% CO2培养箱中培养,待细胞铺满培养瓶后进行传代培养获取纯化的黑素细胞,如图I所示。 [0026] I, normal human melanocytes cultured and passaged: Normal human foreskin trim sake thick, dried 5g / L of Dispase digestion, separation of dermis and epidermis, the epidermis with 2. 5g / L trypsin digestion solution digested into single cell suspensions were set to culture melanocytes (FBS containing 5%, 100U / ml penicillin and 100ug / ml streptomycin, bFGF CDMB153 the medium) at 37 ° C, 5% CO2 incubator, the cells to be confluent after subculture flasks purified melanocytes acquired, as shown I FIG.

[0027] 2、黑素细胞的Masson-Fontana染色鉴定:将第3代细胞悬浮液浓度调至5 X IO3/ml,接种于直径为35mm的培养皿中,每皿接种1ml,24h后进行染色。 The third generation stained cell suspension was adjusted to a concentration of 5 X IO3 / ml, seeded in a petri dish 35mm in diameter, each dish was inoculated 1ml, 24h after: [0027] 2, Masson-Fontana staining melanocytes . 光学显微镜下观察,细胞胞浆中的黑素颗粒呈黑色,细胞核呈红色,证实我们所获得的细胞为黑素细胞。 Observation under an optical microscope, melanin granules in the cytoplasm of black, red nucleus, we confirmed that the cells obtained was melanocytes. 如附图2所示。 As shown in Figure 2.

[0028] 3、MTT法测定不同温度和不同剂量的紫外线照射对体外培养的正常人黑素细胞的增殖活性的影响:选择第3代细胞,以6 X 104/ml的密度,分别接种于13个35mm的培养皿中,每孔I. 5ml。 [0028] 3. Effects of UV measurement at different temperatures and different doses of MTT assay on cultured normal human melanocytes proliferation activity: selects the third passage cells, at a density of 6 X 104 / ml, and were inoculated at 13 in a 35mm culture dish, each well I. 5ml. 细胞接种24h后,其中I组在37°C正常培养(对照组),其中5组分别在39°C、41°C、42°C、43°C、45°C,5个不同温度下干预,每天干预lh,连续干预3d ;另外7组分别在20mJ/cm2>3OmJ/cm2>40mJ/cm2>5OmJ/cm2>7OmJ/cm2>9OmJ/cm2、120mJ/cm27 个不同福照剂量下干预,连续干预3天所有干预组在末次干预20h后,向各培养皿中加入5g/L的MTT溶液,37°C孵育4h。 Cells were seeded 24h, where group I at 37 ° C normal culture (control group), wherein the 5 groups were 39 ° C, 41 ° C, 42 ° C, 43 ° C, 45 ° C, 5 different temperatures intervention intervention daily LH, 3D continuous intervention; 7 Further groups were 20mJ / cm2> 3OmJ / cm2> 40mJ / cm2> 5OmJ / cm2> 7OmJ / cm2> under 9OmJ / cm2,120mJ / cm27 dose Four different intervention, continuous All interventions 3 days after the last intervention group intervention 20h, each dish was added 5g / L of MTT solution, 37 ° C incubation 4h. 弃去培养板内液体加入二甲基亚砜(DMSO),每皿2. 25ml,振荡15min,在492nm波长下检测光密度值并计算增殖率:细胞增殖率)=(实验组平均吸光度-空白对照平均吸光度)/ (对照组平均吸光度-空白对照平均吸光度)X 100%,选择出适合的干预条件作为下一步实验的条件,如附图3,4所示。 Discarded culture liquid inside the panel dimethyl sulfoxide (DMSO), each dish 2. 25ml, shaken 15min, optical density at a wavelength of 492nm and calculated values ​​proliferation rate: cell proliferation rate) = (experimental group average absorbance - blank control mean absorbance) / (mean absorbance of control group - mean absorbance of blank) X 100%, for selected intervention conditions as the conditions for further experiments, as shown in figures 3 and 4.

[0029] 4、酪氨酸酶活性和黑素含量测定:将培养的第4代细胞调至浓度为2 X 105/ml,以每皿4mL分别接种于4个IOOmm培养皿中,24h后分别对采用37°C、5% CO2正常孵育,42°C、5% CO2条件下每天Ih连续干预3d,40mJ/cm2UVB每天I次连续干预3d以及每天42°C、5%CO2条件下干预完成后马上40mJ/cm2UVB干预连续干预3天,所有组在末次干预24h后,分别参考Maeda和Virador等的方法测定细胞酪氨酸酶的活性和黑素含量,选择492nm波长处比色,以空白孔调零,测各孔吸光度值并计算细胞酪氨酸酶活性和黑素含量增长率。 [0029] 4, tyrosinase activity and melanin content determination: The cultured cells were adjusted to a concentration of 4 Generation of 2 X 105 / ml, were inoculated to each dish 4mL IOOmm to four petri dishes were after 24h for using 37 ° C, 5% CO2 normal incubation, 42 ° C, under 5% CO2 condition Ih consecutive interventions per day 3d, 40mJ / cm2UVB day I consecutive interventions 3d daily, and 42 ° C, under 5% CO2 condition intervention is completed the method of the activity and melanin content immediately 40mJ / cm2UVB intervention intervention consecutive 3 days, all groups after the last intervention 24h, respectively, and the reference Maeda Virador like tyrosinase assay, the 492nm wavelength than the color selection to blank well zero, measuring the absorbance of each and calculate the content of tyrosinase activity and growth of melanocytes.

[0030] 细胞酪氨酸酶活性增长率=[(实验组平均吸光度-空白对照平均吸光度)+ (对照组平均吸光度-空白对照平均吸光度)-I] X 100%。 [0030] Cell growth tyrosinase activity = [(mean absorbance of experimental group - mean absorbance of blank) + (mean absorbance of control group - mean absorbance of blank) -I] X 100%.

[0031] 黑素合成增长率=[(实验组吸光度+试验组的细胞数)+ (对照组吸光度+对照组的细胞数)-I] X 100%,获取不同的干预对黑素细胞活性的影响。 [0031] The melanin synthesis rate = [(absorbance of experimental group + the number of cells in test group) + (absorbance of control group + the number of cells in the control group) -I] X 100%, acquiring different interventions melanocyte activity influences. 如附图5所示。 As shown in Figure 5.

[0032] 其次,详尽介绍一下本发明的构造和工作流程 [0032] Next, a detailed description about the structure of the present invention and workflow

[0033] 如附图6、8、9所示,本发明包括红外线、紫外线双光源,可活动支撑杆10及控制台 [0033] As shown in the figures 6,8,9, the present invention including infrared, ultraviolet dual light source, the console 10 and the movable supporting bar

9。 9. 光源发光管置于灯罩6内,灯罩6通过一个活动装置固定在可活动支撑杆10上,电源和控制线路沿支撑杆10走行,支撑杆10的下端固定于控制台9上,控制台9上分别设有红外光源开关I、紫外光源开关2、红外线温控旋钮3、红外光辐照时间设定装置4、紫外光源辐照时间设定装置5。 A light source disposed within the arc tube globe 6, the globe 6 is fixed to the movable support bar 10, and the power supply control lines 10 along the support bar traveling through a movable means, the lower end of the support bar 10 is fixed to the console 9, the console 9 respectively, with an infrared light switch I, UV light source switch 2, the infrared temperature control knob 3, the infrared light irradiation time setting means 4, a source of UV irradiation time setting means 5. 所述红外线温控旋钮通过调控电压来控制红外光源的温度,所述时间设定装置具备控制辐照剂量的计时器。 The infrared temperature control knob to control the temperature of the infrared light by regulating voltage, said time setting means includes a radiation dose control timer.

[0034] 本发明的工作流程:①患者根据需要治疗的部位调节光源位置打开红外线控制开关,根据剂量附表(未给出)调节需要治疗的时间和温度;③等设备工作5分钟后将需要进行辐照治疗的皮损置于剂量附表规定的位置,首先对皮损进行红外线治疗红外线治疗结束后关掉红外光源开关,打开紫外光源开关,同样通过剂量附表(未给出)调节紫外线辐照所用的时间,进行紫外光治疗治疗结束后关掉电源,整个治疗流程结束。 [0034] The workflow of the present invention: ① the source position adjustment according to a patient in need of treatment to open the infrared portion of the control switch, according to the dose schedule (not shown) adjusting the time and temperature in need of treatment; ③ working equipment after 5 minutes lesions dose schedule disposed a predetermined position irradiation treatment, infrared treatment for lesions of the first infrared light source to turn off the switch after infrared treatment, ultraviolet light source switch is opened, by the same dose schedule (not shown) regulating UV the irradiation time used for UV cure treatment after turning off the power, the entire treatment process ends.

[0035] 由于该发明采用了先红外光照射后紫外光照射的序贯疗法,不但能有效地提高白斑皮损的肤色效果,而且也降低了紫外光对皮肤细胞的光毒性作用,减少了光疗的副作用,这也是本发明比目前临床上已应用的其他同类治疗设备的优势所在。 [0035] Since the invention employs a sequential therapy after the first ultraviolet light irradiation of infrared light irradiation, not only can improve the white lesions color effect, but also reduces the toxic effects of ultraviolet light on skin cells, reducing phototherapy side effects, and this is where the present invention is an advantage over other similar devices currently on the clinical treatment has been applied.

Claims (5)

1. 一种治疗白癜风的治疗仪,其特征在于,该治疗仪由红外线和中波紫外线双光源、灯罩、可活动的支撑杆和控制台组成;所述红外线和中波紫外线双光源共同置于所述灯罩中,所述灯罩固定于所述可活动的支撑杆上,所述支撑杆固定于所述控制台上;所述控制台包括双开关控制系统、红外线温度控制系统及时间控制系统;所述红外线和中波紫外线双光源分别和不同的控制开关连接,双开关分别控制两个光源的发光;所述时间控制系统用于设定所述红外线和中波紫外线辐照时间。 1. A method of treating vitiligo treatment, wherein the treatment instrument by the infrared and ultraviolet dual light source, lamp, the support rod may be active, and a console; double the infrared and ultraviolet light source disposed together the globe, the globe is fixed to the movable support bar, the support bar fixed to the console; the console comprises a dual switch control system, control system and time infrared temperature control system; the ultraviolet and infrared light, respectively, double and different control switch is connected, two double switches are controlled emission source; said time control system for setting the infrared and ultraviolet irradiation time.
2.根据权利要求I所述的治疗白癜风的治疗仪,其特征在于,所述红外线和中波紫外线双光源为发光管,置于所述灯罩中,灯罩通过一个活动的装置固定于所述支撑杆上。 The I according to the treatment of vitiligo treatment apparatus as claimed in claim, wherein said infrared and ultraviolet light source is a dual arc tube, is placed in the shade, a movable shade by means fixed to the support rod.
3.根据权利要求I所述的治疗白癜风的治疗仪,其特征在于,所述可活动的支撑杆可做上、下方向上的活动,并能在所述双光源到达了指定位置后能起到固定双光源的作用。 The I according to the treatment of vitiligo treatment apparatus as claimed in claim, wherein said movable support bar do on the bottom up activities, and can reach a specified position in the dual light source can play back fixed double acting source.
4.根据权利要求I所述的治疗白癜风的治疗仪,其特征在于,所述红外线温度控制系统是通过调控电压来控制红外光源的温度。 The I according to the treatment of vitiligo treatment apparatus as claimed in claim, wherein said infrared temperature control system is controlled by an infrared light source temperature regulation voltage.
5.根据权利要求I所述的白癜风的治疗仪,其特征在于,所述时间控制系统具备控制辐照剂量的计时器。 According to claim I of the vitiligo treatment, characterized in that the time control system includes a radiation dose control timer.
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