CN102174597A - Method for producing acetone and butanol through Chinese date fermentation - Google Patents
Method for producing acetone and butanol through Chinese date fermentation Download PDFInfo
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- CN102174597A CN102174597A CN2011100638466A CN201110063846A CN102174597A CN 102174597 A CN102174597 A CN 102174597A CN 2011100638466 A CN2011100638466 A CN 2011100638466A CN 201110063846 A CN201110063846 A CN 201110063846A CN 102174597 A CN102174597 A CN 102174597A
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Abstract
The invention discloses a method for producing acetone and butanol through Chinese date fermentation. The method comprises the following steps: adding water into Chinese dates and pulping to obtain Chinese date pulp; adding a nitrogen source into the Chinese date pulp to form a biological butanol fermentation medium; adjusting the pH value of the medium to 6-7, and sterilizing; after the medium is cooled to 15-30 DEG C, inoculating acetone and butanol clostridium; fermenting to obtain a fermentation liquid; and distilling and rectifying the fermentation liquid to obtain acetone and butanol. The yields of butanol and total solvent in the invention disclosed by the invention are basically consistent with that of the method using starchiness such as corns and the like as raw materials for fermentation; and on the premise of not consuming grain resources, a new biological resource--Chinese date is adopted to produce energy, thereby expanding the variety of biological energy.
Description
Technical field
The present invention relates to the production method of acetone-butanol, be specifically related to a kind of method of utilizing the jujube fermentation production of acetone-butanol.
Background technology
The production method of acetone-butanol mainly contains fermentation method and chemical synthetic method at present.Fermentation method is comparatively sophisticated method, large-scale application is just arranged since 20 beginnings of the century.To the sixties, American-European developed country finds acetone, butanols can be synthetic from oil at an easy rate, so chemical synthetic method replaces the first-selection that fermentation method becomes industrial production acetone, butanols gradually.Because petroleum resources are petered out, International Crude Oil is in high price, add increasingly serious ecological environment problem always, various countries begin to have paid attention to fermentative Production acetone, butanols again but in recent years.
Fermentative Production acetone, butanols mainly use starchy material as raw material, as corn, cassava etc., also can use carbohydrate such as molasses as raw material.Wherein corn is ideal raw material, and it need not add any auxiliary material, needs only the mash that is mixed with 5%-8% through the boiling gelatinization, just can be used as the ideal culture medium of clostridium acetobutylicum.But corn also is a kind of important crops simultaneously, and provision price rises steadily in recent years, and the acetone-butanol production cost is risen.In addition, if a large amount of corn that uses also can bring problems such as grain resource anxiety as fermentation raw material.Therefore, each state all can instead of corn carries out the raw material of fermentation production of acetone, butanols in positive searching.
The distribution range of jujube is very extensive, in various places, Chinese north and south distribution is arranged all.The nutrition of jujube is very abundant, and sugar degree is higher, and bright jujube sugar degree is between 19%-44%, and drying jujube sugar degree can be up to 50%-87%.Jujube also contains mineral elements such as protein, fat and calcium, phosphorus, iron, and the content of its VITAMIN is also higher, and " natural complex " good reputation is arranged.Contained sugar, protein, VITAMIN etc. can satisfy the needs of clostridium acetobutylicum growth fermentation in the jujube, and therefore, jujube is a kind of good acetone butanol fermentation raw material.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing the jujube fermentation production of acetone-butanol.
Purpose of the present invention is achieved through the following technical solutions:
A kind of method of utilizing the jujube fermentation production of acetone-butanol may further comprise the steps:
Get jujube, pull an oar after adding water, obtain the jujube slurry; Add nitrogenous source composition biological butanol fermention medium in the jujube slurry, the pH value of regulating substratum is 6-7, sterilization; After treating that substratum is cooled to 15-30 ℃, insert clostridium acetobutylicum; Fermentation obtains fermented liquid; Fermented liquid obtains acetone, butanols through distillation, rectifying.
Preferably, a kind of method of utilizing the jujube fermentation production of acetone-butanol may further comprise the steps:
(1) preparation jujube slurry: get jujube, remove impurity and fruit stone, pull an oar after adding water, the jujube slurry, the mass concentration of jujube slurry is 3-30%;
(2) biological butanol fermention medium preparation: in the jujube slurry, add nitrogenous source, be made into the biological butanol fermention medium; The nitrogenous source add-on is the 1-3% of substratum quality, and regulating jujube slurry add-on is 5-8% with sugared mass concentration in the control substratum, and regulating medium pH is 6, sterilization;
(3) inoculation: after treating that substratum is cooled to 15-30 ℃, insert clostridium acetobutylicum, inoculum size is 10% of a substratum quality;
(4) fermentation: postvaccinal substratum is placed incubator, keep 30-42 ℃ of constant temperature, fermentation time 40-120 hour, anaerobically fermenting obtained fermented liquid;
(5) fermented liquid obtains acetone, butanols by distillation, rectifying.
The described nitrogenous source of step (2) is organic nitrogen source or mineral compound such as nitrate, ammonium salt such as wheat bran, Semen Maydis powder, dregs of beans, rice bran, peanut cake, extractum carnis, yeast extract paste, peptone, can single interpolation or mix and add;
The mass concentration preferred 6% of sugar in the described substratum of step (2);
The described sterilization of step (2) is 115 ℃ and sterilized 20 minutes down;
The described clostridium acetobutylicum of step (3) includes but not limited to following bacterial strain: ATCC824, ATCC3625, ATCC10132, ATCC8529, ATCC39058, CICC8012;
The described fermentation condition of step (4), 37 ℃ of preferred constant temperature fermented 96 hours.
The present invention has following advantage and effect with respect to prior art:
1, the sugar degree of jujube is very high, is easy to be utilized by clostridium acetobutylicum.The VITAMIN of jujube and mineral element content are abundant simultaneously, help the fermentation growth of clostridium acetobutylicum.In the jujube slurry of certain sugared concentration, add suitable nitrogenous source, and suitable fermentation condition is provided, can make the abundant fermentation production of acetone-butanol of jujube.
2, the present invention does not have particular requirement to the jujube as raw material, can effectively widen the purposes of jujube, and jujube class resource is fully used.
3, butanols output of the inventive method and total solvent output are consistent when being fermenting raw materials with corn and other starches matter substantially, under the prerequisite that does not expend grain resource, adopted a kind of new Biological resources---jujube production capacity in next life source, widened the kind of bioenergy.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited thereto.
Embodiment 1
A kind of method of utilizing the jujube fermentation production of acetone-butanol may further comprise the steps:
A. prepare jujube slurry: get jujube, remove impurity and fruit stone, pull an oar after adding water, the jujube slurry;
B. biological butanol fermention medium preparation: in the jujube slurry, add wheat bran composition biological butanol fermention medium.Regulating jujube slurry add-on is 8% to substratum sugar concentration, and the wheat bran add-on is 1% of a substratum quality, and regulating medium pH is 7,115 ℃ of autoclavings 20 minutes;
C. inoculation: after treating that substratum is cooled to 25 ℃, insert clostridium acetobutylicum strains A TCC824 and ferment, inoculum size is 10% of a substratum quality;
D. fermentation: postvaccinal substratum is placed incubator, keep 42 ℃ of constant temperature, fermentation time 40 hours, anaerobically fermenting obtains fermented liquid;
E. get the 1ml fermented liquid and place centrifuge tube, with the centrifugal 10min of the rotating speed of 10000rpm, with the content of ethanol, acetone, butanols in the gas Chromatographic Determination fermented liquid, the result is as shown in table 1.Fermentation is the result show, fermentation back butanols output can reach 12g/L, and the butanols output when being fermenting raw materials with corn and other starches matter is consistent, and total solvent is also near the level when being fermenting raw materials with corn and other starches matter.
Table 1 strains A TCC824 fermentation result (unit: the g/L fermented liquid)
Embodiment 2
A kind of method of utilizing the jujube fermentation production of acetone-butanol may further comprise the steps:
A. prepare jujube slurry: get jujube, remove impurity and fruit stone, pull an oar after adding water, the jujube slurry;
B. biological butanol fermention medium preparation: in the jujube slurry, add wheat bran and extractum carnis composition biological butanol fermention medium.Regulating jujube slurry add-on is 6% to substratum sugar concentration, and wheat bran and extractum carnis add-on are 2% of substratum quality, and regulating medium pH is 6,115 ℃ of autoclavings 20 minutes;
C. inoculation: after treating that substratum is cooled to 30 ℃, insert clostridium acetobutylicum strains A TCC3625 and ferment, inoculum size is 10% of a substratum quality;
D. fermentation: postvaccinal substratum is placed incubator, keep 37 ℃ of constant temperature, fermentation time 96 hours, anaerobically fermenting obtains fermented liquid;
E. get the 1ml fermented liquid and place centrifuge tube, with the centrifugal 10min of the rotating speed of 10000rpm, with the content of ethanol, acetone, butanols in the gas Chromatographic Determination fermented liquid, the result is as shown in table 2.Fermentation is the result show, butanols output and total solvent output are consistent when being fermenting raw materials with corn and other starches matter substantially.
Table 2 strains A TCC3625 fermentation result (unit: the g/L fermented liquid)
Embodiment 3
A kind of method of utilizing the jujube fermentation production of acetone-butanol may further comprise the steps:
A. prepare jujube slurry: get jujube, remove impurity and fruit stone, pull an oar after adding water, the jujube slurry;
B. biological butanol fermention medium preparation: in the jujube slurry, add wheat bran composition biological butanol fermention medium.Regulating jujube slurry add-on is 6% to substratum sugar concentration, and the wheat bran add-on is 2% of a substratum quality, and regulating medium pH is 6,115 ℃ of autoclavings 20 minutes;
C. inoculation: after treating that substratum is cooled to 20 ℃, insert clostridium acetobutylicum strains A TCC10132 and ferment, inoculum size is 10% of a substratum quality;
D. fermentation: postvaccinal substratum is placed incubator, keep 37 ℃ of constant temperature, fermentation time 96 hours, anaerobically fermenting obtains fermented liquid;
E. get the 1ml fermented liquid and place centrifuge tube, with the centrifugal 10min of the rotating speed of 10000rpm, with the content of ethanol, acetone, butanols in the gas Chromatographic Determination fermented liquid, the result is as shown in table 3.Fermentation is the result show, fermentation is the result show, butanols output and total solvent output are consistent when being fermenting raw materials with corn and other starches matter substantially.
Table 3 strains A TCC10132 fermentation result (unit: the g/L fermented liquid)
Embodiment 4
A kind of method of utilizing the jujube fermentation production of acetone-butanol may further comprise the steps:
A. prepare jujube slurry: get jujube, remove impurity and fruit stone, pull an oar after adding water, the jujube slurry;
B. biological butanol fermention medium preparation: in the jujube slurry, add ammonium nitrate composition biological butanol fermention medium.Key joint jujube slurry add-on is 5% to substratum sugar concentration, and the ammonium nitrate add-on is 3% of a substratum quality, and regulating medium pH is 6.5,115 ℃ of autoclavings 20 minutes;
C. inoculation: after treating that substratum is cooled to 15 ℃, insert clostridium acetobutylicum strains A TCC8529 and ferment, inoculum size is 10% of a substratum quality;
D. fermentation: postvaccinal substratum is placed incubator, keep 30 ℃ of constant temperature, fermentation time 120 hours, anaerobically fermenting obtains fermented liquid;
E. get the 1ml fermented liquid and place centrifuge tube, with the centrifugal 10min of the rotating speed of 10000rpm, with the content of ethanol, acetone, butanols in the gas Chromatographic Determination fermented liquid, the result is as shown in table 4.Fermentation is the result show, fermentation is the result show, butanols output and total solvent output are consistent when being fermenting raw materials with corn and other starches matter substantially.
Table 4 strains A TCC8529 fermentation result (unit: the g/L fermented liquid)
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (7)
1. method of utilizing the jujube fermentation production of acetone-butanol is characterized in that may further comprise the steps:
Get jujube, pull an oar after adding water, obtain the jujube slurry; Add nitrogenous source composition biological butanol fermention medium in the jujube slurry, the pH value of regulating substratum is 6-7, sterilization; After treating that substratum is cooled to 15-30 ℃, insert clostridium acetobutylicum; Fermentation obtains fermented liquid; Fermented liquid obtains acetone, butanols through distillation, rectifying.
2. a kind of method of utilizing the jujube fermentation production of acetone-butanol according to claim 1 is characterized in that may further comprise the steps:
(1) get jujube, remove impurity and fruit stone, pull an oar after adding water, the jujube slurry, the mass concentration of jujube slurry is 3-30%;
(2) in the jujube slurry, add nitrogenous source, be made into the biological butanol fermention medium; The nitrogenous source add-on is the 1-3% of substratum quality, and regulating jujube slurry add-on is 5-8% with sugared mass concentration in the control substratum, and regulating medium pH is 6, sterilization;
(3) treat that substratum is cooled to 15-30 ℃ after, insert clostridium acetobutylicum, inoculum size is 10% of a substratum quality;
(4) postvaccinal substratum is placed incubator, keep 30-42 ℃ of constant temperature, fermentation time 40-120 hour, anaerobically fermenting obtained fermented liquid;
(5) fermented liquid obtains acetone, butanols by distillation, rectifying.
3. a kind of method of utilizing the jujube fermentation production of acetone-butanol according to claim 1 and 2 is characterized in that: described nitrogenous source is more than one mixtures in wheat bran, Semen Maydis powder, dregs of beans, rice bran, peanut cake, extractum carnis, yeast extract paste, peptone, nitrate or the ammonium salt.
4. a kind of method of utilizing the jujube fermentation production of acetone-butanol according to claim 2 is characterized in that: the mass concentration of sugar is 6% in the described substratum of step (2).
5. a kind of method of utilizing the jujube fermentation production of acetone-butanol according to claim 1 and 2 is characterized in that: described sterilization is 115 ℃ and sterilized 20 minutes down.
6. a kind of method of utilizing the jujube fermentation production of acetone-butanol according to claim 1 and 2 is characterized in that: described clostridium acetobutylicum is ATCC824, ATCC3625, ATCC10132, ATCC8529, ATCC39058 or CICC8012.
7. a kind of method of utilizing the jujube fermentation production of acetone-butanol according to claim 2 is characterized in that: the described fermentation condition of step (4) is 37 ℃ of constant temperature, ferments 96 hours.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240300A (en) * | 2008-01-24 | 2008-08-13 | 李世杰 | Production of acetone and butanol by sugar grass straw or/and sweet corn straw fermentation |
| CN101333545A (en) * | 2008-08-05 | 2008-12-31 | 上海凯赛生物技术研发中心有限公司 | Method for producing bio butanol |
| CN101397576A (en) * | 2008-10-13 | 2009-04-01 | 中国科学院广州能源研究所 | Method for fermentation production of acetone-butanol by using wheat starch pulp or wheat starch after extraction of wheat gluten as principal raw material |
| CN101475965A (en) * | 2008-12-29 | 2009-07-08 | 刘明全 | Method for preparing ethanol from Chinese date |
| CN101475966A (en) * | 2008-12-29 | 2009-07-08 | 刘明全 | Method for preparing ethanol from date |
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2011
- 2011-03-17 CN CN2011100638466A patent/CN102174597A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240300A (en) * | 2008-01-24 | 2008-08-13 | 李世杰 | Production of acetone and butanol by sugar grass straw or/and sweet corn straw fermentation |
| CN101333545A (en) * | 2008-08-05 | 2008-12-31 | 上海凯赛生物技术研发中心有限公司 | Method for producing bio butanol |
| CN101397576A (en) * | 2008-10-13 | 2009-04-01 | 中国科学院广州能源研究所 | Method for fermentation production of acetone-butanol by using wheat starch pulp or wheat starch after extraction of wheat gluten as principal raw material |
| CN101475965A (en) * | 2008-12-29 | 2009-07-08 | 刘明全 | Method for preparing ethanol from Chinese date |
| CN101475966A (en) * | 2008-12-29 | 2009-07-08 | 刘明全 | Method for preparing ethanol from date |
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Application publication date: 20110907 |