CN102174507A - Method and system for killing off tumor cells - Google Patents

Method and system for killing off tumor cells Download PDF

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CN102174507A
CN102174507A CN 201110027344 CN201110027344A CN102174507A CN 102174507 A CN102174507 A CN 102174507A CN 201110027344 CN201110027344 CN 201110027344 CN 201110027344 A CN201110027344 A CN 201110027344A CN 102174507 A CN102174507 A CN 102174507A
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particle
magnetic field
magnetic nano
tumour cell
low power
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张国欣
李潇
张岚
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Shanghai Institute of Applied Physics of CAS
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Shanghai Institute of Applied Physics of CAS
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract

The invention discloses a method and system for killing off tumor cells. The method comprises the following step of: treating tumor cells which contain magnetic nanoparticles on cell membranes or in cells through a low-power alternating magnetic field. The system comprises a low-power alternating magnetic field generator and a plurality of magnetic nanoparticles, wherein the magnetic nanoparticles are positioned in the cells or on the cell membranes of the tumor cells and are positioned in a low-power alternating magnetic field generated by the low-power alternating magnetic field generator. By adopting the method for killing off the tumor cells, the tumor cells can be effectively killed off, heat damage is not produced to normal tissues, and high-frequency radiation damage threat is not caused.

Description

A kind of method and system of killing tumour cell
Technical field
The present invention relates to a kind of method and system of killing tumour cell.
Background technology
Because super paramagnetic Fe 3O 4Has outstanding biocompatibility, monodispersity and magnetic response character, make it aspect biochemical, a series of application be arranged, for example: utilize magnetic and biocompatibility, it is improved the tumour cell target by the externally-applied magnetic field effect as magnetic medicine carrier, or be applied to the nmr imaging technique of accurate medical diagnosis; And, utilize magnetic response to make the character of magnetic nanoparticle heat production that corresponding thermotherapy technology is introduced cancer therapy at twentieth century the fifties by alternating magnetic field, promptly the hyperthermia by magnetic nano-particle effectively destroys pathological tissues.
The general magnetic nano-particle that is of a size of 8nm-12nm that uses of typical hyperthermia treatment technology, its utilization enters magnetic nano-particle eddy generation and hysteresis in alternating magnetic field of tumour cell, make the continuous heat production of magnetic nano-particle, thereby the heat that produces makes the tumour cell of local tumor tissue produce irreversible injury because of the overheated pair cell of being killed, reach therapeutic purpose thus, and wherein, the alternating magnetic field power 200W-2000W that uses, frequency 300kHz-1100kHz obviously belongs to the alternating magnetic field of high power, high, make the technological deficiency that the thermotherapy technology also exists some to be difficult to overcome thus when realizing disease treatment, be included in the therapeutic process produce a large amount of heat passage to adjacent tissue and to the thermal damage of healthy tissues, and high frequency magnetic field radiation injury, for example, some case is treated to occur after for some time dizzy in high frequency magnetic field and is caused unreal phenomenon etc., therefore, the defective that exists in view of aforesaid method has significantly been delayed and has directly been killed further developing of tumour cell correlation technique, and this present situation needs to be resolved hurrily.
Summary of the invention
Technical problem to be solved by this invention is that the too high thermotherapy technology that has overcome prior art exists the defective that the healthy tissues around the tumour cell is produced violent thermal damage and high frequency radiation damage, and provide a kind of tumour cell of can effectively killing, can not produce thermal damage, the method and system of killing tumour cell that does not have the high frequency radiation damage to threaten to healthy tissues.
The method of killing tumour cell of the present invention comprises the steps: that the tumour cell that will contain magnetic nano-particle on the cytolemma or in the cell gets final product through the processing of low power alternating magnetic field.
Among the present invention, described tumour cell is the various tumour cells of existing medical field, as cervical cancer cell (Hela), liver cancer cell (Hep3B) etc.
Among the present invention, as long as described magnetic nano-particle is the described magnetic nanoparticle with magnetic of this area routine, as Fe 3O 4, FeOOH, γ-Fe 2O 3, CoFe 2O 4Magnetic nano-particle etc., that preferable is Fe 3O 4Magnetic nano-particle.
Among the present invention, described magnetic nano-particle is that this area routine is described, can also can be the magnetic nano-particle through base group modification for without the magnetic nano-particle of modifying.Described not modified magnetic nano-particle is meant that the magnetic nano-particle particle surface does not link the target biology molecule.Describedly be meant the magnetic nano-particle of surface link target biology molecule through the base group modification magnetic nano-particle, it makes magnetic nano-particle have better biocompatibility by base group modification and easilier combines with tumour cell; Wherein, described is that this area routine is described through the surface of base group modification magnetic nano-particle link target biology molecule, as rgd peptide, monoclonal antibody, α vβ 3Integrin receptor etc.Described is that this area routine is described through the magnetic nano-particle of base group modification or not modified magnetic nano-particle, all commercially availablely get or make according to this area ordinary method, preparation surface connects the target biology molecule in the prior art magnetic nano-particle reference all can, as patent CN200710038353.0 " preparation method of amido modified magnetic nano-particle and immunomagnetic isolation reagent "; Periodical literature " Synthesis of acicular a-FeOOH particles at a very high pH. " (Materials Letters, 2002,54:108-113); " Iron oxide nanoparticles as a drug delivery vehicle for MRI monitored magnetic targeting of brain t
Among the present invention, that the size of described magnetic nano-particle is preferable is 50nm-500nm, and that better is 200nm.
Among the present invention, what the length-to-diameter ratio of described magnetic nano-particle was preferable is 1: 1-1: 50, and better is 1: 4-1: 10.The length-to-diameter ratio of magnetic nano-particle of the present invention all can realize the object of the invention in preferred range, but consider that length-to-diameter ratio is big more, effect tumour cell effect is stronger but blood circulation time is preferential declines to a great extent, take all factors into consideration both factors, the length-to-diameter ratio of described magnetic nano-particle is 1: 4-1: had better technique effect at 10 o'clock.
Among the present invention, described magnetic nano-particle is arranged on the cytolemma of tumour cell or the implementation of cell is this area routine operation, generally magnetic nano-particle and tumour cell co-cultivation is got final product.Wherein, described cultivation is this area routine operation, and the nutrient solution that relates to, culture condition are all suitably adjusted according to actual culturing cell, is example with the Hela cell, can contain 5%CO under 37 ℃ 2Incubator in cultivate, with DMEM high glucose medium or RPMI1640 substratum etc.Wherein, described not modified magnetic nano-particle is generally realized being arranged on the cytolemma of tumour cell or cell by cytophagy; Described magnetic nano-particle through base group modification is general then to be realized being arranged on the cytolemma of tumour cell or cell by transfection, wherein, all know the corresponding different target tumor cell that the different target biology molecule of magnetic nano-particle finishing is different for magnetic nano-particle those skilled in the art through base group modification.As: cervical cancer cell (Hela) and magnetic nano particle sub-surface coupling α vβ 3The integrin receptor correspondence; Liver cancer cell (Hep3B) is corresponding with magnetic nano particle sub-surface coupling monoclonal antibody CEA etc.Wherein, described cytophagy or transfection are the conventional term in this area.Described cytophagy is that phalangeal cell is directly engulfed the allosome particulate matter.Described transfection is meant the process of inserting somatocyte or clone by the DNA small segment.
Among the present invention, described low power alternating magnetic field is the conventional low power alternating magnetic field in this area, can be produced by the conventional low power alternating magnetic field generator in this area.The power of described low power alternating magnetic field is low power, and described low power is that this area routine is described, general those skilled in the art's default scoping be greater than 0W and≤10W, that preferable is 1W-5W, that better is 3W.The range of frequency of described low power alternating magnetic field is preferable be greater than 0kHz and≤50kHz, that better is 25kHz-45kHz, that best is 35kHz.
Among the present invention, that the treatment time of described low power alternating magnetic field is preferable is 30min-3h, and that better is 2h.
The invention still further relates to a kind of system that kills tumour cell and comprise a low power alternating magnetic field generator and some magnetic nano-particles, described magnetic nano-particle is arranged on the cell or cytolemma of tumour cell, and is arranged in the low power alternating magnetic field that the low power alternating magnetic field generator produces.
Among the present invention, described magnetic nano-particle, tumour cell, low power alternating magnetic field are all as previously mentioned.
Among the present invention, described low power alternating magnetic field generator is the conventional described low power alternating magnetic field generator in this area, and preferable low power alternating magnetic field generator comprises: alternating-current input unit, coil and U-shaped carbon steel.Wherein, to be this area routine described for described alternating-current input unit, coil and U-shaped carbon steel.
Agents useful for same of the present invention and raw material be all commercially available getting except that specified otherwise.
On the basis that meets this area general knowledge, each above-mentioned among the present invention technical characterictic optimum condition can arbitrary combination obtain preferred embodiments.
Positive progressive effect of the present invention is: the method for killing tumour cell of the present invention will be arranged on the cytolemma of tumour cell or the magnetic nano-particle of cell is handled at the low power alternating magnetic field and can be killed tumour cell effectively, its magnetic nano-particle heats up and is no more than 2 ℃, frequency that the low power alternating magnetic field relates to and power are on 10 times of the too high thermotherapy technical parameters of prior art, can not produce thermal damage to healthy tissues, there is not the high frequency radiation damage to threaten, can be used for the methodology checking, lay solid theories and experiment basis for the research and the application that only have clinical use to kill tumour cell, be significant.
Description of drawings
Fig. 1 is the synoptic diagram of the system that kills tumour cell of embodiment 1.
Fig. 2 is the magnetic nano-particle electromicroscopic photograph of embodiment 2.
Fig. 3 is the SEM collection of illustrative plates after the magnetic nano-particle of embodiment 2 enters tumour cell.
Fig. 4 is the SEM collection of illustrative plates of the tumour cell that contains magnetic nano-particle after the low power alternating magnetic field is handled of embodiment 2.
Fig. 5 is the cytoactive graphic representation of the tumour cell that contains magnetic nano-particle after the low power alternating magnetic field is handled of embodiment 2.
Fig. 6 is the permeability cell graphic representation of the tumour cell that contains magnetic nano-particle after the low power alternating magnetic field is handled of embodiment 2.
Embodiment
Mode below by embodiment further specifies the present invention, but does not therefore limit the present invention among the described scope of embodiments.
Embodiment 1
As shown in Figure 1, the invention provides a kind of system that kills tumour cell, it comprises low power alternating magnetic field generator and some magnetic nano-particles 6, the cell that described magnetic nano-particle 6 is arranged in tumour cell 5 (also can be positioned on the cytolemma of tumour cell, do not draw among the figure), and be arranged in the low power alternating magnetic field 4 that the low power alternating magnetic field generator produces; Described low power alternating magnetic field generator comprises alternating-current input unit 1, coil 2 and U-shaped carbon steel 3, and alternating-current input unit 1 links to each other with coil 2, and coil 2 is wound in U-shaped carbon steel 3.
Embodiment 2
1, Fe 3O 4The preparation of magnetic nano-particle
The preparation of bar-shaped magnetic nano-particle: 10ml deionized water and a small amount of benzene are added in the reactor, then with the FeSO of 0.139g 47H 2O and 0.270g FeCl 36H 2O and 5ml quadrol under agitation add reactor, fully stir 5 minutes after, sealed reactor is 120 ℃ of down reactions 12 hours, reactor naturally cools to room temperature, product is collected with magnet and is cleaned respectively three times with deionized water and ethanol.Following dry 12 hours in final product and the vacuum at 60 degrees centigrade.After testing, shown in Fig. 2 B, institute's synthesizing magnetic nanoparticle length is 200 nanometers, and diameter is 40 nanometers, and length-to-diameter ratio is 5.
The preparation of spherical magnetic nano-particle: the 10ml deionized water is added in the reactor.Then with 0.81g FeCl 36H 2O is dissolved in the 25ml ethylene glycol, adds Potassium ethanoate, fully stir 5 minutes after, sealed reactor is 200 degrees centigrade of reactions 24 hours down.Reactor naturally cools to room temperature, and product is collected with magnet and cleaned respectively three times with deionized water and ethanol.Following dry 12 hours in final product and the vacuum at 60 degrees centigrade.After testing, shown in Fig. 2 A, institute's synthesizing magnetic nano particle diameter is 200 nanometers, and length-to-diameter ratio is 1.
2, low power alternating magnetic field generator
Low power alternating magnetic field generator in the present embodiment wherein obtains the output rating of 3W as described in the embodiment 1 by the control alternating current voltage, by alternating-current input unit such as alternating current motor alternative frequency is set at 35kHz.
3, cell cultures and contain the preparation of magnetic nano-particle nutrient solution
The Hela cell of exponential phase of growth is cultured in the DMEM nutrient solution that contains 10% foetal calf serum, and ambient conditions is 37 ℃ and has mixed 5%CO 2Mixed atmosphere.In MTT test and cellular uptake test, cell cultures in culture dish more than 2 hours so that fully adherent, when 80% when above of cell covering culture dish, promptly can test.
Magnetic nano-particle soaks degerming in a day in straight alcohol, then with PBS flushing 3 times; Aseptic magnetic nano-particle and DMEM contain the magnetic nano-particle nutrient solution and are kept at 4 ℃ of conditions according to the respective concentration uniform mixing.
After the cell fraction of coverage reaches 80%, add and contain spherical or bar-shaped magnetic nano-particle concentration the nutrient solution that is 100 μ g/ml, co-cultivation is 3 hours respectively, 6 hours, 12 hours, after 20 hours, remove nutrient solution and wash cell 3 times with PBS, the collecting cell and the upper strata liquid of going out, the magnetic nano-particle that is absorbed with dissolving with hydrochloric acid cell and cell, ferrous ion with luxuriant and rich with fragrance alloxazine (ferrozine) complexing form can usefulness spectrography detection by quantitative coloring matter as can be known, the picked-up total amount of magnetic nano-particle is 4.143 times of ferrous iron amount.
Wherein, after the magnetic nano-particle of present embodiment entered tumour cell, the SEM collection of illustrative plates after promptly being absorbed by tumour cell is (spherical magnetic nano-particle was A, and bar-shaped magnetic nano-particle is B) as shown in Figure 3.
4, the cell of magnetic nano-particle has been absorbed in processing under the alternating magnetic field
With above-mentioned, in containing the magnetic nano-particle nutrient solution, cultivate after 20 hours, with PBS flushing cell three times to remove unconjugated magnetic particle, free cell is by five equilibrium to 5 dactylethrae afterwards, under action of alternating magnetic field, handled 0 minute respectively then, 10 minutes, 30 minutes, 60 minutes and 120 minutes, wherein, the result of cell after 60min handles as shown in Figure 4, after alternating magnetic field is handled, it is sparse that cytolemma obviously becomes, and cell tissue is destructurized.
As model, because the self-reparing capability of cell, the mobile need of magnetic nano-particle are enough strong and the lasting sufficiently long time further so that it is irreversible to injure, and therefore, preferably handle the long period in magnetic field with the Hela cell for present embodiment.Simultaneously, as shown in Figure 4, this treating processes of present embodiment has been avoided heat effect, makes the result can only reflect the pattern effect of magnetic nano-particle.
5, handle the test of front and back cytoactive
The cytoactive of present embodiment is that (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) measures by mtt assay.After the alternating magnetic field of corresponding time is handled, the MTT of 20ml is joined in the corresponding dactylethrae, continue then to cultivate 4 hours, remove nutrient solution afterwards the MTT product of separating out is dissolved in the 150ml methyl-sulphoxide, shook on shaking table 10 minutes so that fully be dissolved in DMSO, the amount of the MTT that is reduced can obtain by testing its absorbancy with microplate reader under 570nm.
Cytoactive is obtained by the absorbancy of processed back cell and the absorbancy contrast of untreated cell, net result, curve as shown in Figure 5, magnetic nano-particle cytoactive after 120 minutes alternating magnetic fields are handled is as follows: spherical magnetic nano-particle cytoactive has reduced by 13.9%, and bar-shaped magnetic nano-particle action effect is obvious, and cytoactive has reduced by 30.9%.
6, handle the test of front and back permeability of cell membrane
The penetrance of cytolemma is that the ratio that is colored cell after being dyeed 3 minutes by trypan blue gets.Cell is respectively through 0 minute under the alternating magnetic field, 10 minutes, 30 minutes, after handling in 60 minutes and 120 minutes, come the pair cell nuclear staining by trypan blue to penetrating the damaged cell film, therefore can characterize penetrance by the ratio that is colored cell changes, further, being colored cell can observe under ordinary optical microscope and count, the result as shown in Figure 6, every milliliter of spherical and bar-shaped magnetic nano-particle 100 microgram are after handling by alternating magnetic field, 39.47% bar-shaped magnetic nano-particle is dyed blueness, and spherical magnetic nano-particle only has 15.13% to be dyed blueness.

Claims (10)

1. method of killing tumour cell is characterized in that: its tumour cell that comprises the steps: to contain on the cytolemma or in the cell magnetic nano-particle is handled through the low power alternating magnetic field and is got final product.
2. the method for killing tumour cell as claimed in claim 1 is characterized in that: described magnetic nano-particle is Fe 3O 4, FeOOH, γ-Fe 2O 3Or CoFe 2O 4Magnetic nano-particle; And/or described magnetic nano-particle is not modified magnetic nano-particle or through the magnetic nano-particle of base group modification.
3. the method for killing tumour cell as claimed in claim 1 is characterized in that: the size of described magnetic nano-particle is 50nm-500nm, and that preferable is 200nm.
4. the method for killing tumour cell as claimed in claim 1 is characterized in that: the length-to-diameter ratio of described magnetic nano-particle is 1: 1-1: 50, and preferable is 1: 4-1: 10.
5. the method for killing tumour cell as claimed in claim 1 is characterized in that: the power of described low power alternating magnetic field for greater than 0W and≤10W, that preferable is 1W-5W, that better is 3W.
6. the method for killing tumour cell as claimed in claim 1 is characterized in that: the range of frequency of described low power alternating magnetic field for greater than 0kHz and≤50kHz, that preferable is 25kHz-45kHz, that better is 35kHz.
7. the method for killing tumour cell as claimed in claim 1 is characterized in that: the treatment time of described low power alternating magnetic field is 30min-3h, and that preferable is 2h.
8. system that kills tumour cell, it is characterized in that: it comprises a low power alternating magnetic field generator and some magnetic nano-particles, described magnetic nano-particle is arranged on the cell or cytolemma of tumour cell, and is arranged in the low power alternating magnetic field that the low power alternating magnetic field generator produces.
9. the system that kills tumour cell as claimed in claim 8 is characterized in that: described magnetic nano-particle is as claim 2,3 or 4 described magnetic nano-particles.
10. the system that kills tumour cell as claimed in claim 8 is characterized in that: described low power alternating magnetic field generator comprises: alternating-current input unit, coil and U-shaped carbon steel; The alternating magnetic field that described low power alternating magnetic field generator produces is as claim 5 or 6 described alternating magnetic fields.
CN 201110027344 2011-01-25 2011-01-25 Method and system for killing off tumor cells Pending CN102174507A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013178839A1 (en) * 2012-05-31 2013-12-05 Investigaciones, Desarrollos E Innovaciones Tat Iberica, S.L. Method and device for the destruction of cells with uncontrolled proliferation
CN106520548A (en) * 2016-10-17 2017-03-22 中国医科大学附属盛京医院 Device for radioactive ion to radiate and induce cell for damaging at short distance
CN108439487A (en) * 2018-04-19 2018-08-24 山东师范大学 A kind of magnetic nano-particle and preparation method thereof can be used for cancer target magnetic therapy
WO2024079713A1 (en) * 2022-10-13 2024-04-18 Magheals Private Limited A high frequency magnetic induction device for the treatment of oral cancer

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
《中国优秀硕士学位论文全文数据库》 20101215 唐静波 肿瘤磁纳米热疗用交变磁场发生仪器实验样机的研制与Fe_3O_4纳米磁流体磁热效应研究 第24页第2段 8-10 , *
《功能材料》 20061231 赵东林等 纳米Fe3O4粒子的制备及其在变交磁场下的发热性能 全文 8-10 第37卷, *
《南京医科大学学报（自然科学版）》 20101231 居会祥等 外加极低频交变磁场照射下致吸附FeOx颗粒不同类型肝癌细胞凋亡研究 第1709页右栏第1.2.2至1.2.6 8-10 第30卷, 第12期 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013178839A1 (en) * 2012-05-31 2013-12-05 Investigaciones, Desarrollos E Innovaciones Tat Iberica, S.L. Method and device for the destruction of cells with uncontrolled proliferation
CN106520548A (en) * 2016-10-17 2017-03-22 中国医科大学附属盛京医院 Device for radioactive ion to radiate and induce cell for damaging at short distance
CN106520548B (en) * 2016-10-17 2018-09-25 中国医科大学附属盛京医院 A kind of device for the radiation-induced cellular damage of radion short distance
CN108439487A (en) * 2018-04-19 2018-08-24 山东师范大学 A kind of magnetic nano-particle and preparation method thereof can be used for cancer target magnetic therapy
WO2024079713A1 (en) * 2022-10-13 2024-04-18 Magheals Private Limited A high frequency magnetic induction device for the treatment of oral cancer

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Application publication date: 20110907