CN102174499A - Resin-enzyme composite catalyst and preparation method thereof - Google Patents
Resin-enzyme composite catalyst and preparation method thereof Download PDFInfo
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- 239000002131 composite material Substances 0.000 title claims abstract description 117
- 239000003054 catalyst Substances 0.000 title claims abstract description 114
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 102000004190 Enzymes Human genes 0.000 claims abstract description 213
- 108090000790 Enzymes Proteins 0.000 claims abstract description 213
- 229920005989 resin Polymers 0.000 claims abstract description 146
- 239000011347 resin Substances 0.000 claims abstract description 146
- 238000003756 stirring Methods 0.000 claims abstract description 43
- 238000001035 drying Methods 0.000 claims abstract description 30
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000003456 ion exchange resin Substances 0.000 claims abstract description 14
- 229920003303 ion-exchange polymer Polymers 0.000 claims abstract description 14
- 230000007935 neutral effect Effects 0.000 claims abstract description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 120
- 239000000243 solution Substances 0.000 claims description 97
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 60
- 239000000872 buffer Substances 0.000 claims description 60
- 230000003301 hydrolyzing effect Effects 0.000 claims description 51
- 108010029541 Laccase Proteins 0.000 claims description 32
- 238000011010 flushing procedure Methods 0.000 claims description 30
- 229920001429 chelating resin Polymers 0.000 claims description 15
- 108010054320 Lignin peroxidase Proteins 0.000 claims description 11
- 239000011148 porous material Substances 0.000 claims description 6
- 108010035722 Chloride peroxidase Proteins 0.000 claims description 3
- 102000018832 Cytochromes Human genes 0.000 claims description 3
- 108010052832 Cytochromes Proteins 0.000 claims description 3
- -1 E.C. 1.1.99.1 Proteins 0.000 claims description 3
- OKIZCWYLBDKLSU-UHFFFAOYSA-M N,N,N-Trimethylmethanaminium chloride Chemical compound [Cl-].C[N+](C)(C)C OKIZCWYLBDKLSU-UHFFFAOYSA-M 0.000 claims description 3
- 238000005342 ion exchange Methods 0.000 claims description 3
- 239000003729 cation exchange resin Substances 0.000 claims 2
- 238000000034 method Methods 0.000 abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 11
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000002255 enzymatic effect Effects 0.000 abstract description 2
- 239000008363 phosphate buffer Substances 0.000 abstract 2
- 230000003197 catalytic effect Effects 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 61
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 56
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 56
- 239000007979 citrate buffer Substances 0.000 description 56
- 239000001488 sodium phosphate Substances 0.000 description 56
- 229910000162 sodium phosphate Inorganic materials 0.000 description 56
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 56
- 230000009514 concussion Effects 0.000 description 44
- 239000000463 material Substances 0.000 description 34
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 32
- RLBIQVVOMOPOHC-UHFFFAOYSA-N parathion-methyl Chemical group COP(=S)(OC)OC1=CC=C([N+]([O-])=O)C=C1 RLBIQVVOMOPOHC-UHFFFAOYSA-N 0.000 description 32
- 239000011159 matrix material Substances 0.000 description 29
- 125000003368 amide group Chemical group 0.000 description 28
- 239000011780 sodium chloride Substances 0.000 description 28
- 230000015556 catabolic process Effects 0.000 description 19
- 238000006731 degradation reaction Methods 0.000 description 19
- 150000001768 cations Chemical class 0.000 description 18
- 238000004042 decolorization Methods 0.000 description 18
- 239000000975 dye Substances 0.000 description 18
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 18
- 229940107698 malachite green Drugs 0.000 description 18
- 229910052799 carbon Inorganic materials 0.000 description 16
- 239000003957 anion exchange resin Substances 0.000 description 15
- 238000002386 leaching Methods 0.000 description 10
- ASOKPJOREAFHNY-UHFFFAOYSA-N 1-Hydroxybenzotriazole Chemical compound C1=CC=C2N(O)N=NC2=C1 ASOKPJOREAFHNY-UHFFFAOYSA-N 0.000 description 9
- NPZTUJOABDZTLV-UHFFFAOYSA-N hydroxybenzotriazole Substances O=C1C=CC=C2NNN=C12 NPZTUJOABDZTLV-UHFFFAOYSA-N 0.000 description 9
- 150000003384 small molecules Chemical class 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 239000012071 phase Substances 0.000 description 7
- VGVRPFIJEJYOFN-UHFFFAOYSA-N 2,3,4,6-tetrachlorophenol Chemical class OC1=C(Cl)C=C(Cl)C(Cl)=C1Cl VGVRPFIJEJYOFN-UHFFFAOYSA-N 0.000 description 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 108010093096 Immobilized Enzymes Proteins 0.000 description 4
- 238000004132 cross linking Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000002210 silicon-based material Substances 0.000 description 4
- 229920001661 Chitosan Polymers 0.000 description 3
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000003463 adsorbent Substances 0.000 description 3
- 238000006555 catalytic reaction Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 238000005554 pickling Methods 0.000 description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 238000005411 Van der Waals force Methods 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000009881 electrostatic interaction Effects 0.000 description 2
- AMWRITDGCCNYAT-UHFFFAOYSA-L hydroxy(oxo)manganese;manganese Chemical compound [Mn].O[Mn]=O.O[Mn]=O AMWRITDGCCNYAT-UHFFFAOYSA-L 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229910052787 antimony Inorganic materials 0.000 description 1
- WATWJIUSRGPENY-UHFFFAOYSA-N antimony atom Chemical compound [Sb] WATWJIUSRGPENY-UHFFFAOYSA-N 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 125000004218 chloromethyl group Chemical group [H]C([H])(Cl)* 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000010954 inorganic particle Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses a resin-enzyme composite catalyst and a preparation method thereof, belonging to the field of composite materials. The method comprises the following steps: dissolving a target enzyme in a phosphate buffer with pH value being neutral so as to obtain a solution A with concentration of 0.2mg/mL-10mg/mL; selecting a suitable large-hole type ion exchange resin as a carrier according to isoelectric points of an enzyme molecule; mixing and stirring the solution A and the resin carrier at room temperature; and taking out the resin, washing the surface of the resin by utilizing the phosphate buffer and drying the resin to obtain the resin-enzyme composite catalyst. The preparation method provided by the invention has the advantages of simplicity in operation, easiness in control and less loss of enzymatic activity; the fixed-load enzyme composite catalyst prepared by the method provided by the invention ensures that the stability of ordinary enzyme is greatly improved on the basis of maintaining the original catalytic activity of the enzyme molecules, can be applied to a water phase system and has the characteristic of good leaching-resisting property within a wide pH range and under high ionic strength.
Description
Technical field
The present invention relates to a kind of composite catalyst and preparation method thereof, a kind of resin-enzyme composite catalyst and preparation method thereof of saying so more specifically.
Background technology
Enzyme is a kind of high-performance bio catalyzer, have that speed of response is fast, mild condition, reaction thoroughly, substrate had an advantage such as specificity.But in common solution reaction system, enzymic catalytic reaction exist enzyme to be difficult to reclaim and recycle, anti-reaction conditions (temperature, pH, salinity etc.) impact capacity is poor, easily inactivation, product such as are difficult to separate at shortcoming, these shortcomings have greatly limited enzyme directly applying in biocatalysis field.For overcoming these shortcomings, scientist begins enzyme is carried out immobilized preparation carrier-enzyme composite catalyst, existing so far many reports.Common enzyme composite catalyzing agent carrier comprises mesoporous silicon material, gac, diatomite, chitosan, resin etc. at present.
With regard to fixing means, using maximum is the method for covalent cross-linking.This method is carried out covalent bond with enzyme molecule and carrier by linking agent by chemical bridging method, and common carrier comprises molecular sieve, resin etc., and the general supported quantity of the composite catalyst that makes is bigger, Stability Analysis of Structures; But chemical reaction is often comparatively fierce in the fixation procedure, complicated operation, level of response is difficult to control, a lot of enzymes have very large loss of activity (Lee CH in immobilized process, Lin TS, Mou CY. Mesporous materials for encapsulating enzymes. Nano Today, 2009,4:165-179).Another kind of common physisorphtion rigid condition is simple, easy handling, common carrier comprises mesoporous silicon material, gac, diatomite, chitosan etc., the composite catalyst enzyme that makes loss alive is less, but the enzyme molecule combines instability with carrier, easy leaching loss in reaction soln.People such as Wang YJ are carrier with mesoporous silicon material BMS, carrier-enzyme matrix material by the physisorphtion preparation, surpassing 70% enzyme molecule can stripping once more in solution (Mesoporous silica spheres as supports for enzyme immobilization and encapsulation. Chemistry of Materials 2005,17:953-961).How to develop and need the technical bottleneck that overcomes when simple, gentle preparation method realizes that the stabilization of enzyme is immobilized and become preparation high-performance enzyme composite catalyst badly.
Macroreticular ion exchange resin has good physical strength, abundant pore structure, good ion-exchange capacity; obtained widespread use in fields such as water treatment, Industrial Catalysis, environment protection, these structural performances also make it possess good solid support material primary condition.In recent years, the Pan Bingcai of Nanjing University professor seminar with macroporous ion exchange resin as carrier, by the internal surface deposition technique that inorganic particles such as nano-sized iron oxide, manganese oxide particle is immobilized in the resin duct, succeed in developing serial organic-inorganic nano compound adsorbent, successfully solved the deep purifying difficult problem of multiple pollutents such as micro heavy, arsenic, selenium, phosphorus, antimony in the water body.Its prepared compound adsorbent is because the poly-confinement effect of the distinctive net of resin nano pore structure, make it in solution, have good anti-leaching loss performance (Pan BJ et al. Development of polymer-based nanosized hydrated ferric oxides (HFOs) for enhanced phosphate removal from water effluents. Water Res, 2009,43:4421-4429; Patent publication No. CN101804333A; CN101643289).The degree of depth removal that large hole cation exchanger resin D001 went up and be used for cupric ion also once was fixed in macromolecule organic polymine (PEI) by simple pickling process in this seminar, this composite adsorbing material is because the factors such as space winding between electrostatic interaction, Van der Waals force and carrier and the PEI macromolecular chain, has very good anti-leaching loss performance equally, relevant achievement has been published in international research journal Environ. Sci. Technol.(2010,44 (9), 3508) on.
Existing at present with the immobilized report on resin of enzyme.The method that mostly adopts in the report has following several: earlier resin is mixed with enzyme liquid, react after for some time again with glutaraldehyde carry out crosslinked (
J. Agric. Food. Chem. 2010,58,488; Material Leader: a research piece of writing. 23 the 9th phases of volume of in September, 2009 (descending), 50); Earlier resin is handled with glutaraldehyde, and then mixed (food and fermentation industries, 2004 30 the 2nd phases of volume, 10 with enzyme liquid; ); Earlier resin is modified, is connected groups such as some chloromethyls, amino or carboxyl, again the covalent attachment by these groups and enzyme molecule make enzyme be fixed on the resin (
J. Chem. Technol. Biotechnol. 2003, 78,891;
Applied Catalysis B:Environmental. 2003,42,131; Food Chemistry.2009,112,992).Shortcomings such as loss that as can be seen, these methods all have complicated operation, reaction is violent and degree is difficult to control, the enzymatic structure influence is big, enzyme is lived is serious.
In addition, also have by simple absorption method enzyme is immobilized in the report of resin, these methods can be summarized as follows: adopt hydrophobic nonpolar macroporous adsorption resin to pass through the absorption method immobilized enzyme as carrier, no matter this method synthesizes is still used, all be confined in the system of organic solvent, moisture content is had strict requirement, limited its application (biotechnology journal at aqueous phase, 2006 22 1 phases of volume, 114; He'nan University of Technology's journal (natural science edition), 2007 the 28th 3 phases of volume, 68; The processing of farm products academic periodical, 2010 3 phases, 23); Adopting macroporous adsorbent resin at aqueous phase is carrier, by the absorption method immobilized enzyme, main by hydrogen bond, van der waals forces immobilized enzyme, this method resin polarity is strong more, fixed effect is poor more, is difficult to be applied to the water system, and enzyme stability is improved less (chemical engineering, 2009 37 4 phases of volume, 8); With resin diethylin ethyl hydroxyl ethyl ester (DEAE-E/H) is carrier, main by the hydrogen bond action immobilized enzyme, the composite catalyst of its preparation is less to the raising of enzyme stability, and be subject to pH value of solution and ionic strength affect and make the enzyme leaching loss in solution, thereby be not suitable in the water system using (chemical reaction engineering and technology equally, 2005 21 1 phases of volume, 60).And the stronger electrostatic force of utilization effect, thereby by simple pickling process enzyme is fixed on the strong polar macroporous ion exchange resin obtain high stability, can be applicable to the water system, the composite catalyst of anti-leaching loss excellent performance but do not see open report as yet.
Summary of the invention
1. invent the technical problem that will solve
The main method of preparation carrier-enzyme composite catalyst comprises covalent cross-linking method and physisorphtion at present.The composite catalyst structure that covalent cross-linking method makes is comparatively stable, and loss is big but method complexity, enzyme are lived; Though physisorphtion is simple to operate, the anti-leaching poor performance of the composite catalyst that makes, stability are not high.The purpose of this invention is to provide a kind of macroporous ion exchange resin-enzyme composite catalyst and preparation method thereof, this preparation method is simple, and the macroporous ion exchange resin for preparing-enzyme composite catalyst fixed amount is big, physical strength good, can be applicable to the water system, anti-leaching loss excellent performance, stability are stronger.
2. technical scheme
The principle of the invention: with the solid support material of macroreticular ion exchange resin as enzyme, the pore structure of resin rich can make things convenient for the macromole enzyme to enter its inside, duct, the charged group of resin surface can and the enzyme molecule between produce electrostatic force, its distinctive mesh nano pore structure can make the molecular chain of enzyme twine mutually with it and carry out more stable combining, and the immobilized important foundation that provides of the stabilization of enzyme molecule may be provided these factors.By simple pickling process that the enzyme molecule is immobilized on the macroporous ion exchange resin carrier, prepare that successfully a kind of fixed amount is big, physical strength good, can have in the leaching loss excellent performance water system, anti-, resin-enzyme composite catalyst that reaction stability is strong.
Technical scheme of the present invention is as follows:
A kind of resin-enzyme composite catalyst and preparation method thereof the steps include:
(1) target enzyme is dissolved in the pH value and obtains solution A in the neutral phosphoric acid buffer, its concentration range is 0.2-10 mg/mL;
(2), select suitable macroreticular ion exchange resin as carrier according to the iso-electric point of enzyme molecule;
(3) solution A is mixed stirring with resin carrier under room temperature, it is 10-500 mL that average every gram resin needs the volume of solution A;
(4) take out ion exchange resin, be neutral phosphoric acid buffer flushing resin surface with the pH value, resin is dried in the shade obtains resin-enzyme composite catalyst again.
PH value in step (1) and the step (4) typically refers to pH=7.0 for neutrality.
In the step (1) specific enzyme be molecular weight greater than 10 k dalton, the bioprotein Deng Dian Dian ≦ 5.5 Huo ≧ 8.0 is as laccase, E.C. 1.1.99.1, chloroperoxidase, organophosphor hydrolytic enzyme, cytochrome C etc.;
Select the method for resin carrier to be in the step (2): to the enzyme (as laccase, E.C. 1.1.99.1, chloroperoxidase, lignin peroxidase etc.) of iso-electric point ≦ 5.5, can select macroporous type anionite-exchange resin is carrier, preferred D201 resin, D301 resin, IRA900 resin; To the polymer enzyme (as organophosphor hydrolytic enzyme, cytochrome C etc.) of iso-electric point ≧ 8.0, can select large hole cation exchanger resin is carrier, preferred D001 resin, D101 resin, Amberlite 200 resins, Lewatit Sp-210 resin.
3. beneficial effect
Macroporous ion exchange resin provided by the invention-enzyme composite catalyst and preparation method thereof is than existing carrier-enzyme composite catalyst and preparation method thereof, have some outstanding advantages: (1) than covalent cross-linking method, and preparation method provided by the invention is simple to operate, be easy to control, enzyme is lived, and loss is little; (2) than the carrier-enzyme composite catalyst by the physisorphtion preparation such as general carrier such as mesoporous silicon material, gac, diatomite, chitosan, the composite catalyst of the present invention's preparation has good anti-leaching loss performance, high handling stability; (3) than nonpolar macroporous adsorption resin and weak-base ion-exchange resin carrier-enzyme composite catalyst by hydrogen bond, the preparation of Robert Van de Walle power, the composite catalyst of the present invention's preparation is owing to the stronger electrostatic interaction of utilization effect has higher stability, have good wetting ability simultaneously and can be conveniently used in the water system, and can under the pH of broad scope and higher ionic strength, have stronger anti-leaching loss performance.
Embodiment
Below further specify the present invention by specific embodiment
Embodiment 1
Laccase (molecular weight 69kD, iso-electric point 3～4) is dissolved in the phosphoric acid buffer of pH=7.0 and obtains laccase solution, concentration is 2mg/mL; Get 0.20 g macroporous anion exchange resin D201 and mix, at room temperature stir 48 h with 20mL laccase solution; Then take out the phosphoric acid buffer flushing resin surface of macroporous anion exchange resin, obtain resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.
By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D201,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-15%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free laccase activity lost when initial below 50%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free laccase has lost about 40% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L malachite green dyestuff and stir (the small molecules amboceptor 1-hydroxyl-benzo-triazole that adds 0.08mL 1mM) at 25 ℃ down, in 24h, decolour 40%.D201 under the similarity condition dyes no any decolorization to malachite green.Reuse 25 times, percent of decolourization does not obviously descend.
Embodiment 2
Basic step is with embodiment 1, be specially: get 0.20 g macroporous anion exchange resin D301, contain 2mg/mL laccase (molecular weight 69kD with 20mL, iso-electric point 3～4), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of macroporous anion exchange resin, obtain resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.
By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D301,1600～1700,1220～1330,600-700 cm-1 place all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-10%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free laccase activity lost when initial below 50%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free laccase has lost about 40% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L malachite green dyestuff and stir (the small molecules amboceptor 1-hydroxyl-benzo-triazole that adds 0.08mL 1mM) at 25 ℃ down, in 24h, decolour 60%.D301 under the similarity condition dyes no any decolorization to malachite green.Reuse 25 times, percent of decolourization does not obviously descend.
Embodiment 3
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin Amberlite IRA900, contain 2mg/mL laccase (molecular weight 69kD with 20mL, iso-electric point 3～4), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h and then take out the phosphoric acid buffer flushing resin surface of macroporous anion exchange resin, obtain resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with Amberlite IRA900,1600～1700,1220～1330,600-700 cm-1 place all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-18%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free laccase activity lost when initial below 50%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free laccase has lost about 40% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L malachite green dyestuff and stir (the small molecules amboceptor 1-hydroxyl-benzo-triazole that adds 0.08mL 1mM) at 25 ℃ down, in 24h, decolour 55%.Amberlite IRA900 under the similarity condition dyes no any decolorization to malachite green.Reuse 25 times, percent of decolourization does not obviously descend.
Embodiment 4
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin D201, contain 0.5mg/mL laccase (molecular weight 69kD with 20mL, iso-electric point 3～4), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D201,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-8%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free laccase activity lost when initial below 50%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free laccase has lost about 40% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L malachite green dyestuff and stir (the small molecules amboceptor 1-hydroxyl-benzo-triazole that adds 0.08mL 1mM) at 25 ℃ down, in 24h, decolour 30%.D201 under the similarity condition dyes no any decolorization to malachite green.Reuse 25 times, percent of decolourization does not obviously descend.
Embodiment 5
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin D201, contain 10mg/mL laccase (molecular weight 69kD with 20mL, iso-electric point 3～4), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D201,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-20%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free laccase activity lost when initial below 50%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free laccase has lost about 40% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L malachite green dyestuff and stir (the small molecules amboceptor 1-hydroxyl-benzo-triazole that adds 0.08mL 1mM) at 25 ℃ down, in 24h, decolour 70%.D201 under the similarity condition dyes no any decolorization to malachite green.Reuse 25 times, percent of decolourization does not obviously descend.
Embodiment 6
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin D201, contain 2mg/mL laccase (molecular weight 69kD with 50mL, iso-electric point 3～4), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D201,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-18%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free laccase activity lost when initial below 50%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free laccase has lost about 40% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L malachite green dyestuff and stir (the small molecules amboceptor 1-hydroxyl-benzo-triazole that adds 0.08mL 1mM) at 25 ℃ down, in 24h, decolour 80%.D201 under the similarity condition dyes no any decolorization to malachite green.Reuse 25 times, percent of decolourization does not obviously descend.
Embodiment 7
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin D201, contain 10mg/mL laccase (molecular weight 69kD with 80mL, iso-electric point 3～4), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D201,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-20%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free laccase activity lost when initial below 50%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free laccase has lost about 40% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L malachite green dyestuff and stir (the small molecules amboceptor 1-hydroxyl-benzo-triazole that adds 0.08mL 1mM) at 25 ℃ down, in 24h, decolour 80%.D201 under the similarity condition dyes no any decolorization to malachite green.Reuse 25 times, percent of decolourization does not obviously descend.
Embodiment 8
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin D201, contain 5mg/mL laccase (molecular weight 69kD with 80mL, iso-electric point 3～4), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D201,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-14%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free laccase activity lost when initial below 50%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free laccase has lost about 40% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L malachite green dyestuff and stir (the small molecules amboceptor 1-hydroxyl-benzo-triazole that adds 0.08mL 1mM) at 25 ℃ down, in 24h, decolour 60%.D201 under the similarity condition dyes no any decolorization to malachite green.Reuse 25 times, percent of decolourization does not obviously descend.
Embodiment 9
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin D201, contain 2mg/mL lignin peroxidase (molecular weight 40kD with 20mL, iso-electric point 3.5), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D201,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-9%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free lignin peroxidase activity lost when initial below 40%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free lignin peroxidase has lost about 30% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L 2,6-two chlorophenols stir (hydrogen peroxide that adds the about 1mM of 0.08mL) down at 25 ℃, degrade in 5h〉95%.D201 under the similarity condition is to 2, and 6-two chlorophenols only remove＜and 5%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 10
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin D201, contain 2mg/mL lignin peroxidase (molecular weight 40kD with 50mL, iso-electric point 3.5), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D201,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-13%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free lignin peroxidase activity lost when initial below 40%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free lignin peroxidase has lost about 30% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L 2,6-two chlorophenols stir (hydrogen peroxide that adds the about 1mM of 0.08mL) down at 25 ℃, degrade in 5h〉95%.D201 under the similarity condition is to 2, and 6-two chlorophenols only remove＜and 5%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 11
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin D201, contain 0.5 mg/mL lignin peroxidase (molecular weight 40kD with 50mL, iso-electric point 3.5), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D201,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-7%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free lignin peroxidase activity lost when initial below 40%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free lignin peroxidase has lost about 30% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L 2,6-two chlorophenols stir (hydrogen peroxide that adds the about 1mM of 0.08mL) down at 25 ℃, degrade in 5h〉95%.D201 under the similarity condition is to 2, and 6-two chlorophenols only remove＜and 5%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 12
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin D001, contain 2 mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 20mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D001,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-5%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.D201 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 13
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin D001, contain 2 mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 50mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D001,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-13%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.D201 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 14
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin D001, contain 0.5 mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 50mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D001,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-7%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.D201 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 15
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin D001, contain 10 mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 20mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D001,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-17%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.D201 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 16
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin D101, contain 2 mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 20mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D101,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-6%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.D101 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 17
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin D101, contain 2 mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 50mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D101,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-9%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.D101 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 18
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin D101, contain 10mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 20mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D101,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-8%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.D101 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 19
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin D101, contain 0.5 mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 50mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with D101,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-4%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.D101 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 20
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin Amberlite 200, contain 10mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 20mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with Amberlite 200,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-7%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.200 couples of Amberlite under the similarity condition are to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 21
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin Amberlite 200, contain 2mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 20mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with Amberlite 200,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-5%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.200 couples of Amberlite under the similarity condition are to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 22
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin Amberlite 200, contain 2mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 50mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with Amberlite 200,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-10%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.200 couples of Amberlite under the similarity condition are to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 23
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin Amberlite 200, contain 0.5mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 50mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with Amberlite 200,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-7%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.200 couples of Amberlite under the similarity condition are to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 26
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin Lewatit Sp-210, contain 2mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 20mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with Lewatit Sp-210,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-10%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.Lewatit Sp-210 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 27
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin Lewatit Sp-210, contain 0.5mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 50mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with Lewatit Sp-210,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-8%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.Lewatit Sp-210 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 28
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin Lewatit Sp-210, contain 10 mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 20mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with Lewatit Sp-210,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-17%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.Lewatit Sp-210 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 29
Basic step is with embodiment 1, be specially: 0.20 g large hole cation exchanger resin Lewatit Sp-210, contain 5 mg/mL organophosphor hydrolytic enzyme (molecular weight 39kD with 20mL, iso-electric point 8.3), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with Lewatit Sp-210,1600～1700,1220～1330,600-700 cm
-1The place has all had more the charateristic avsorption band of amide group.This material is concussion 7 days under the room temperature in the Tris-HCl buffered soln of the Sodium phosphate dibasic-citrate buffer solution of pH 3～7 and pH 7～9, and the enzyme amount of its maximum loss is＜1-9%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Mixed 8 days in the Sodium phosphate dibasic-citrate buffer solution of matrix material and pH3～7 and the Tris-HCl buffered soln of pH 7～9, its relative enzyme live no significantly sacrificing (under the similarity condition free organophosphor hydrolytic enzyme activity lost when initial below 30%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (organophosphor hydrolytic enzyme has lost about 60% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L parathion-methyl is stirred down at 35 ℃, in 2h, degrade 97%.Lewatit Sp-210 under the similarity condition is to parathion-methyl clearance＜1%.Reuse 25 times, degradation rate does not obviously descend.
Embodiment 30
Basic step is with embodiment 1, be specially: 0.20 g macroporous anion exchange resin IRA900, contain 2mg/mL laccase (molecular weight 69kD with 30mL, iso-electric point 3～4), the phosphoric acid buffer of pH=7.0 mixes, at room temperature stir 48 h, then take out the phosphoric acid buffer flushing resin surface of resin, promptly prepare resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with pH=7.0.By Fourier transform infrared spectroscopy (FT-IR) scanning, find that this composite catalyst compares with IRA900,1600～1700,1220～1330,600-700 cm-1 place all had more the charateristic avsorption band of amide group.This material shook 7 days under the room temperature in Sodium phosphate dibasic-citrate buffer solution of pH 3～7, and the enzyme amount of its maximum loss is＜1-9%.The room temperature concussion is 7 days in the NaCl of 1M solution, enzyme amount＜1% of loss.Matrix material mixed 8 days with the Sodium phosphate dibasic-citrate buffer solution of pH3～7, its relative enzyme live no significantly sacrificing (under the similarity condition free laccase activity lost when initial below 50%); Leave standstill 20 h under 40 ℃, its enzyme is lived does not also have significantly sacrificing (free laccase has lost about 40% activity under the similarity condition).
Get 10 mg composite catalysts and 10mL 50mg/L malachite green dyestuff and stir (the small molecules amboceptor 1-hydroxyl-benzo-triazole that adds 0.08mL 1mM) at 25 ℃ down, in 24h, decolour 60%.IRA900 under the similarity condition dyes no any decolorization to malachite green.Reuse 25 times, percent of decolourization does not obviously descend.
Claims (6)
1. resin-enzyme composite catalyst and preparation method thereof the steps include:
(1) target enzyme is dissolved in the pH value and obtains solution A in the neutral phosphoric acid buffer, its concentration range is 0.2-10 mg/mL;
(2) according to the iso-electric point of enzyme molecule, selecting suitable macroreticular ion exchange resin is carrier;
(3) solution A is mixed stirring with macroreticular ion exchange resin under room temperature, it is 10-500 mL that average every gram resin needs the volume of solution A;
(4) take out macroreticular ion exchange resin, for neutral phosphoric acid buffer flushing resin surface, obtain resin-enzyme composite catalyst again with drying in the shade under the resin room temperature with the pH value.
2. preparation method according to claim 1 is characterized in that the enzyme in the step (1) is the bioprotein of molecular weight greater than 10 K dalton, Dian Dian ≦ 5.5 Huo ≧ 8.0.
3. preparation method according to claim 2, the selection that it is characterized in that resin carrier in the step (2) is according to being: to the enzyme of iso-electric point ≦ 5.5, select macroporous type anionite-exchange resin; To the enzyme of iso-electric point ≧ 8.0, select large pores cation exchange resin.
4. preparation method according to claim 3 is characterized in that macroporous type anionite-exchange resin is D201 resin, D301 resin or Amberlite IRA900 resin in the step (2); Large pores cation exchange resin is D001 resin, D101 resin, Amberlite 200 resins or Lewatit Sp-210 resin.
5. according to claim 3 or 4 described preparation methods, it is characterized in that the enzyme of iso-electric point in the step (2) ≦ 5.5 is laccase, E.C. 1.1.99.1, chloroperoxidase or lignin peroxidase; Enzyme Deng Dian Dian ≧ 8.0 is organophosphor hydrolytic enzyme or cytochrome C.
6. macroporous ion exchange resin-enzyme composite catalyst of preparing of the described preparation method of claim 1.
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| CN111057702A (en) * | 2019-12-30 | 2020-04-24 | 北京电子科技职业学院 | Immobilized biological enzyme and application thereof in remediation of organophosphorus pesticide contaminated soil |
| CN117088985A (en) * | 2023-05-31 | 2023-11-21 | 南京大学 | BRET-based methyl parathion biosensor, construction method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493696A (en) * | 2002-10-29 | 2004-05-05 | 上海理工大学 | Disposable immobilized enzyme column suitable for agricultural fast detection |
| JP2009125038A (en) * | 2007-11-27 | 2009-06-11 | Gun Ei Chem Ind Co Ltd | Immobilized enzyme complex and carbohydrate solution prepared by using the same |
| CN101560511A (en) * | 2009-05-19 | 2009-10-21 | 江南大学 | Method of immobilization of fructosyl transferase by macroporous anion resin |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493696A (en) * | 2002-10-29 | 2004-05-05 | 上海理工大学 | Disposable immobilized enzyme column suitable for agricultural fast detection |
| JP2009125038A (en) * | 2007-11-27 | 2009-06-11 | Gun Ei Chem Ind Co Ltd | Immobilized enzyme complex and carbohydrate solution prepared by using the same |
| CN101560511A (en) * | 2009-05-19 | 2009-10-21 | 江南大学 | Method of immobilization of fructosyl transferase by macroporous anion resin |
Non-Patent Citations (2)
| Title |
|---|
| 《水处理技术》 19850831 王佳兴 固定化酶与离子交换树脂 2-4 1-5 第11卷, 第4期 2 * |
| 《生物工程学报》 20091225 王燕华等 D301树脂固定化假丝酵母脂肪酶 2037-2040 1-6 第25卷, 第12期 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111057702A (en) * | 2019-12-30 | 2020-04-24 | 北京电子科技职业学院 | Immobilized biological enzyme and application thereof in remediation of organophosphorus pesticide contaminated soil |
| CN117088985A (en) * | 2023-05-31 | 2023-11-21 | 南京大学 | BRET-based methyl parathion biosensor, construction method and application thereof |
| CN117088985B (en) * | 2023-05-31 | 2024-05-10 | 南京大学 | BRET-based methyl parathion biosensor, construction method and application thereof |
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