CN102161971A - Fermentation inoculum for producing cellulase and hemicellulase - Google Patents
Fermentation inoculum for producing cellulase and hemicellulase Download PDFInfo
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- CN102161971A CN102161971A CN2011101074583A CN201110107458A CN102161971A CN 102161971 A CN102161971 A CN 102161971A CN 2011101074583 A CN2011101074583 A CN 2011101074583A CN 201110107458 A CN201110107458 A CN 201110107458A CN 102161971 A CN102161971 A CN 102161971A
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- microbial inoculum
- hemicellulase
- fusarium
- cellulase
- alcaligenes faecalis
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- 229940059442 hemicellulase Drugs 0.000 title claims abstract description 28
- 108010002430 hemicellulase Proteins 0.000 title claims abstract description 28
- 108010059892 Cellulase Proteins 0.000 title claims abstract description 24
- 229940106157 cellulase Drugs 0.000 title claims abstract description 23
- 238000000855 fermentation Methods 0.000 title claims description 9
- 230000004151 fermentation Effects 0.000 title claims description 9
- 239000002054 inoculum Substances 0.000 title abstract description 5
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 31
- 241001489200 Fusarium poae Species 0.000 claims abstract description 22
- 241000223192 Fusarium sporotrichioides Species 0.000 claims abstract description 22
- 241001520808 Panicum virgatum Species 0.000 claims abstract description 21
- 241001673062 Achromobacter xylosoxidans Species 0.000 claims abstract description 20
- 241000588813 Alcaligenes faecalis Species 0.000 claims abstract description 20
- 229940005347 alcaligenes faecalis Drugs 0.000 claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 claims abstract description 11
- 239000010902 straw Substances 0.000 claims abstract description 9
- 108090000790 Enzymes Proteins 0.000 claims description 22
- 102000004190 Enzymes Human genes 0.000 claims description 22
- 229940088598 enzyme Drugs 0.000 claims description 22
- 239000003795 chemical substances by application Substances 0.000 claims description 17
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 8
- 239000001913 cellulose Substances 0.000 claims description 8
- 229920002678 cellulose Polymers 0.000 claims description 8
- 235000011187 glycerol Nutrition 0.000 claims description 8
- 238000010521 absorption reaction Methods 0.000 claims description 7
- 230000001332 colony forming effect Effects 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 4
- 241000195940 Bryophyta Species 0.000 claims description 2
- 238000004806 packaging method and process Methods 0.000 claims description 2
- 239000003415 peat Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 17
- 230000000694 effects Effects 0.000 description 15
- 230000001580 bacterial effect Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 239000002689 soil Substances 0.000 description 6
- 244000005700 microbiome Species 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
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- 150000004823 xylans Chemical class 0.000 description 3
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- 241000590020 Achromobacter Species 0.000 description 2
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
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- 239000001963 growth medium Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
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- 239000012978 lignocellulosic material Substances 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
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- CBMPTFJVXNIWHP-UHFFFAOYSA-L disodium;hydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].OP([O-])([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O CBMPTFJVXNIWHP-UHFFFAOYSA-L 0.000 description 1
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- FYKDNWHPKQOZOT-UHFFFAOYSA-M sodium;dihydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].OP(O)([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O FYKDNWHPKQOZOT-UHFFFAOYSA-M 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 229960003487 xylose Drugs 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses a microbial inoculum for producing cellulase and hemicellulase by a natural lignocellulose material. The microbial inoculum for producing the cellulase and the hemicellulase comprises the following active ingredients: Achromobacter xylosoxidans, Alcaligenes faecalis, Fusarium sporotrichioides and Fusarium poae. In the invention, microbes in the microbial inoculum for producing the cellulase and the hemicellulase have a strong synergistic effect so that a stable ecological system can be formed in an autologous mode. The microbial inoculum is especially developed for an energy crop-Panicum virgatum but is not limited to production of the cellulase and the hemicellulase by use of the Panicum virgatum, and meanwhile the inoculum can be also used for producing the cellulase and the hemicellulase by waste crop straw.
Description
Technical field
The present invention relates to a kind of microbial inoculum of be used to ferment switchgrass lignocellulose material produce cellulase and hemicellulase.
Background technology
Along with expanding economy, human increasing to the demand of non-renewable fossil energies such as oil, Sweet natural gas, coal, produced problems such as energy security, consumption of petroleum, Global warming thus.These problems impel countries in the world to accelerate research to safety, cleaning, reproducible substitute energy.Alcohol fuel is the current research and the alternative energy of using a kind of cleaning that all obtains remarkable progress, but it is produced and mainly depends on starch and saccharide raw material at present, these two kinds of prepared usings relate to people and animals and strive the problem that grain is striven ground, and the higher food prices problem of bringing thus manifests.Cheap raw material beyond the exploitation grain is great to producing the alcohol fuel technical meaning.
Switchgrass (Panicum virgatum) is a kind of perennial herb C4 crop, belongs to one of lignocellulose-like biomass raw material.Have the output height, barren-resistant, drop into low, many characteristics such as adaptability is strong.In nearly in the past 20 years time, successively researched and developed utilization as a kind of huge equivalent material of energy potentiality of producing by the U.S., Canada, EU countries, China has carried out introducing a fine variety of switchgrass and growth characteristics investigation work, but the transformation of switchgrass bio-ethanol is just at the early-stage, yet there are no the microbial inoculum that utilizes switchgrass production of cellulose enzyme and hemicellulase specially.
Ligno-cellulosic materials such as switchgrass are converted into the alcoholic acid process to be comprised: steps such as the separation of raw materials pretreatment, enzymic hydrolysis, sugar-fermenting, xylogen, alcoholic acid recovery and purifying.At present, all exist many technology and cost restriction in each step of these conversions.Hydrolysis is one of key step wherein, cellulase and hemicellulase with its action condition gentleness, pollute characteristics such as few and in lignocellulose ethanol conversion process, play an important role, but enzyme production cost height, the low heavy industrialization that is seriously restricting the cellulosic ethanol conversion of transformation efficiency are used at present, become the bottleneck of industrialization technology.
The traditional cellulase and the production of hemicellulase mainly are at the good product enzyme single strain of occurring in nature screening, again by certain mutagenesis means, obtain the zymogenic bacteria kind that large-scale industrialization is produced.But to be that multiple microorganism is collaborative finish in the degraded of lignocellulose in the physical environment, and the mutual symbiosis of these microorganisms forms a stable little ecosystem.In the specific ecosystem, synergy mutually between the microorganism also conditions each other, and the ratio between the lytic enzyme that make to produce is in a kind of state of the suitableeest degraded, and produce the microorganism of enzyme in case from its little ecosystem, separate, will destroy this optimum proportion.Therefore based on ligocellulose degradation in the physical environment, making up from body stable compound fermenting agent production of cellulose and hemicellulase is the key of dealing with problems.
Summary of the invention
Purpose of the present invention is for providing the fermenting agent of a kind of production of cellulose enzyme and hemicellulase.
The activeconstituents of microbial inoculum provided by the present invention comprises Achromobacter xylosoxidans, Alcaligenesfaecalis, Fusarium sporotrichioides, Fusarium poae.
In the described fermenting agent, the colony forming unit number of described Achromobacter xylosoxidans, Alcaligenes faecalis, Fusarium sporotrichioides, Fusarium poae is than being (4-6): (1-3): (6-10): (1-2), be specially 4: 1: 6: 1 or 6: 3: 10: 2.
Described Achromobacter xylosoxidans specifically can be Achromobacter xylosoxidans ATCC15173; Described Alcaligenes faecalis specifically can be Alcaligenes faecalis ATCC15554; Described Fusarium sporotrichioides specifically can be Fusarium sporotrichioides ATCC26533; Described Fusarium poae specifically can be Fusarium poae ATCC15654;
The activeconstituents of cellulase production microbial inoculum provided by the present invention also can only be made up of Achromobacter xylosoxidansATCC15173, Alcaligenes faecalis ATCC15554, Fusarium sporotrichioides ATCC26533, Fusarium poae ATCC15654.
In the described fermenting agent, described Achromobacter xylosoxidans, Alcaligenes faecalis, Fusarium sporotrichioides, Fusarium poae can distinguish independent packaging, also can mix.
Fermenting agent of the present invention can be liquid preparation, comprises glycerine and EDTA, and the proportioning of described activeconstituents, glycerine and EDTA is (1-1.2) * 10
11CFU: (1-5) g glycerine: 1g EDTA.
Fermenting agent of the present invention can be solid preparation.Add absorption carrier in the aforesaid liquid microbial inoculum, as the peat composed of rotten mosses or switchgrass powder or straw powder, can obtain solid preparation, the proportioning of described activeconstituents and absorption carrier is 1 * 10
6CFU: (1-5) g absorption carrier.
Microbial inoculum of the present invention can ferment switchgrass production of cellulose enzyme and hemicellulase.
The prepared microbial inoculum of the present invention is preferential but be not limited only to utilize switchgrass production of cellulose enzyme and hemicellulase, and its fermented substrate also comprises ligno-cellulosic materials such as agricultural crop straw.Described microbial inoculum has high synergetic effect, forms the stable ecosystem from body, can breed fast in fermentation system and produce enzyme, and operate simple and easy, with low cost.
Embodiment
The screening of activeconstituents in embodiment 1, the fermenting agent
The composition of MCD substratum (g/L): NaNO
32g, KH
2PO
41g, MgSO
47H
2O 1g, KCl 0.5g, FeSO
40.01g, agar 15g, switchgrass (sole carbon source) 10g.
For liquid nutrient medium, join in the substratum after switchgrass pulverized 2mm sieve, all the other compositions are identical.
The sample (sample source: the deadwood that rots, dead leaf, the switchgrass of rotting) that is used at first screen of 0.5g is linked into respectively in Modified Czapek Dox (MCD) agar of 100ml.Behind 30 ℃ of cultivation 14d, the switchgrass of 0.05g being covered with microorganism is transferred to succeeding transfer culture on the new substratum.Choose the rapid deliquescing of switchgrass, Mierocrystalline cellulose, the hemicellulose culture rapidly of degrading, screening activeconstituents.
By the molecular ecology method set up 16S rDNA, 26S rDNA D1/D2 district clone library is studied the culture of above-mentioned acquisition, the result shows that the composition bacterium in the above-mentioned culture is mainly Achromobacter xylosoxidans, Alcaligenes faecalis, Fusarium sporotrichioides, Fusarium poae.
The fermenting agent of embodiment 2, cellulase-producing and hemicellulase.
One, the preparation of microbial inoculum
Achromobacter xylosoxidans ATCC15173; Alcaligenes faecalis ATCC15554; Fusarium sporotrichioides ATCC26533; Fusarium poae ATCC15654 is all available from American Type Culture Collection.Bacterium source is respectively soil, ight soil, beans shell and barley seed.
Bacterial strain activation: Achromobacter xylosoxidans ATCC15173 and Alcaligenes faecalisATCC15554 are inoculated into nutrient agar medium (every liter of substratum composition: peptone 10g respectively, extractum carnis 3g, NaCl 5g, agar 15g, distilled water 1000ml, pH 7.4) on, 2d cultivated for 27 ℃; Fusarium sporotrichioides ATCC26533 and Fusariumpoae ATCC15654 are inoculated into respectively on the PDA (every liter of substratum is formed: potato 200g, glucose 20g, agar 15g, distilled water 1000mL, pH nature), cultivate 4d for 27 ℃.
The activatory bacterial strain is inoculated in respectively in the corresponding liquid substratum: Achromobacter xylosoxidansATCC15173 and Alcaligenes faecalis ATCC15554 are inoculated in 50ml nutrient broth (every liter of substratum composition: peptone 10g respectively, extractum carnis 3g, NaCl 5g, distilled water 1000ml, pH 7.4) in, 150rpm shaking culture 2d; Fusarium sporotrichioides ATCC26533 and Fusarium poae ATCC15654 are inoculated in the 50ml PDA liquid nutrient medium 150rpm shaking culture 4d respectively.Get Achromobacter xylosoxidans ATCC15173 4ml, Alcaligenes faecalisATCC15554 1ml, Fusarium sporotrichioides ATCC26533 6ml, Fusarium poae ATCC15654 1ml mix, making the final colony forming unit number ratio of four kinds of bacterium is 4: 1: 6: 1, and the colony forming unit number in this mixed-culture medium is 1 * 10
8CFU/ml.
With above-mentioned mixed fermentation liquid, glycerine and EDTA according to 1 * 10
3The proportioning of ml: 5g: 1g is mixed, and obtains liquid bacterial agent A.
Two, the activity of cellulase-producing and hemicellulase microbial inoculum
Getting the liquid bacterial agent A 1ml for preparing is inoculated in the 100ml MCD substratum, at 27 ℃ of following 150rpm shaking culture 6d, switching is gone in the 1L fermentor tank, inoculum size is 10%, temperature is 27-29 ℃, and pH is 6.0, and dissolved oxygen (DO) is controlled at 30%-50%, every 24h sampling (fermented supernatant fluid), measure enzyme as follows and live.
Cellulase (endoglucanase) activity determination method: adopt the DNS method to measure, drawing 0.5ml enzyme liquid adds in the test tube, Xylo-Mucine (CMC-Na) solution (1g CMC-Na is dissolved in 0.05M citric acid-disodium phosphate soln of 100ml pH4.8) that adds 1ml 1% again, 60 ℃ of insulation 30min.Add 3mlDNS reagent, boiling water bath heating 5min is cooled to room temperature with tap water, adds water and is settled to 25ml, measures the reducing sugar amount of its generation.
An enzyme activity unit (U) is defined as: per minute hydrolysis CMC-Na produces the required enzyme amount of 1 μ g glucose.
The measuring method of hemicellulase: draw 0.5ml enzyme liquid and add in the test tube, add the xylan solution (the 1g xylan is dissolved in citric acid-sodium dihydrogen phosphate of the 0.05M of 100ml pH6.0) of 1ml 1% again, 60 ℃ of insulation 5min.Add 3mlDNS reagent, boiling water bath heating 5min is cooled to room temperature with tap water, adds water and is settled to 25ml, measures the reducing sugar amount of its generation.
An enzyme activity unit (U) is defined as: the per minute hydrolyzed xylan produces the required enzyme amount of 1 μ g wood sugar.
The aforesaid method triplicate, the result is as shown in the table, and the data in the table are the enzyme mean+SD alive that every ml fermented liquid is produced.
Microbial inoculum A fermentation switchgrass cellulase-producing activity and hemicellulase activity
The fermenting agent of embodiment 3, cellulase-producing and hemicellulase.
One, the preparation of microbial inoculum
Achromobacter xylosoxidans ATCC15173; Alcaligenes faecalis ATCC15554; Fusarium sporotrichioides ATCC26533; Fusarium poae ATCC15654 is all available from American Type Culture Collection.Bacterium source is respectively soil, ight soil, beans shell and barley seed.
Bacterial strain activation: referring to embodiment 2
The activatory bacterial strain is inoculated in respectively in the corresponding liquid substratum: Achromobacter xylosoxidans ATCC15173 and Alcaligenes faecalis ATCC15554 are inoculated in respectively in the 50ml nutrient broth, 150rpm shaking culture 2d; Fusarium sporotrichioides ATCC26533 and Fusariumpoae ATCC15654 are inoculated in the 50mlPDA liquid nutrient medium 150rpm shaking culture 4d respectively.Get Achromobacter xylosoxidans ATCC15173 6ml, Alcaligenes faecalis ATCC15554 3ml, Fusarium sporotrichioides ATCC26533 10ml, Fusariumpoae ATCC15654 2ml mix, making the final colony forming unit number ratio of four kinds of bacterium is 6: 3: 10: 2, and the colony forming unit number in this mixed-culture medium is 1.2 * 10
8CFU/ml.
With above-mentioned mixed fermentation liquid, glycerine and EDTA according to 1 * 10
3The proportioning of ml: 5g: 1g is mixed, and obtains liquid bacterial agent B.
Two, the activity of cellulase-producing and hemicellulase microbial inoculum
Getting the zymogenic bacteria agent B 1ml for preparing is inoculated in the 100ml MCD substratum, at 27 ℃ of following 150rpm shaking culture 6d, switching is gone in the 1L fermentor tank, inoculum size is 10%, temperature is 27-29 ℃, and pH is 5.0-6.0, and dissolved oxygen (DO) is controlled at 30%-50%, every 24h sampling (fermented supernatant fluid), measure enzyme as follows and live.
Cellulase activity and hemicellulase activity are measured with reference to embodiment 2.
The aforesaid method triplicate, the result is as shown in the table, and the data in the table are the enzyme mean+SD alive that every ml fermented liquid is produced.
Microbial inoculum B fermentation switchgrass cellulase-producing activity and hemicellulase activity
Embodiment 4, bacteria fermentation rice straw cellulase-producing and hemicellulase.
One, the preparation of microbial inoculum
Achromobacter xylosoxidans ATCC15173; Alcaligenes faecalis ATCC15554; Fusarium sporotrichioides ATCC26533; Fusarium poae ATCC15654 is all available from American Type Culture Collection.Bacterium source is respectively soil, ight soil, beans shell and barley seed.
With reference to embodiment 3 preparation liquid bacterial agent B.
Two, the activity of cellulase-producing and hemicellulase microbial inoculum
Getting the zymogenic bacteria agent B for preparing is inoculated in 100ml MCD (carbon source is replaced by the rice straw of results behind the seed by the switchgrass) substratum, at 28 ℃ of following 150rpm shaking culture 6d, switching is gone in the 1L fermentor tank (rice straw is a sole carbon source), inoculum size is 10%, temperature is 27-29 ℃, and pH is 5.0-6.0, and dissolved oxygen (DO) is controlled at 30%-50%, every 24h sampling (fermented supernatant fluid), measure enzyme as follows and live.
Cellulase and hemicellulose enzyme activity determination are with reference to embodiment 2.
Microbial inoculum B fermentation rice straw cellulase-producing activity and hemicellulase activity
Claims (10)
1. the fermenting agent of production of cellulose enzyme and hemicellulase, its activeconstituents comprises: Achromobacter xylosoxidans, Alcaligenes faecalis, Fusarium sporotrichioides, Fusarium poae.
2. microbial inoculum according to claim 1 is characterized in that: the colony forming unit number ratio of described Achromobacter xylosoxidans, Alcaligenes faecalis, Fusarium sporotrichioides, Fusariumpoae is: (4-6): (1-3): (6-10): (1-2).
3. microbial inoculum according to claim 2 is characterized in that: the colony forming unit number ratio of described Achromobacter xylosoxidans, Alcaligenes faecalis, Fusarium sporotrichioides, Fusariumpoae is: 4: 1: 6: 1 or 6: 3: 10: 2.
4. according to arbitrary described microbial inoculum among the right 1-3, it is characterized in that: described Achromobacter xylosoxidans is Achromobacter xylosoxidans ATCC15173; Described Alcaligenes faecalis is Alcaligenes faecalis ATCC15554; Described Fusarium sporotrichioides is Fusarium sporotrichioides ATCC26533; Described Fusarium poae is Fusarium poae ATCC15654.
5. according to arbitrary described microbial inoculum among the claim 1-4, it is characterized in that: comprise glycerine and EDTA in the described microbial inoculum.
6. microbial inoculum according to claim 5 is characterized in that: the proportioning of described activeconstituents, glycerine and EDTA is (1-1.2) * 10
11CFU: (1-5) g glycerine: 1g EDTA.
7. according to the microbial inoculum described in the claim 6, it is characterized in that: comprise absorption carrier in the described microbial inoculum, described absorption carrier is the peat composed of rotten mosses or switchgrass powder or straw powder.
8. according to the microbial inoculum described in the claim 7, it is characterized in that: the proportioning of described activeconstituents and absorption carrier is 1 * 10
6CFU: (1-5) g absorption carrier.
9. according to arbitrary described microbial inoculum among the claim 1-8, it is characterized in that: Achromobacter xylosoxidans ATCC15173, Alcaligenes faecalis ATCC15554, Fusarium sporotrichioides ATCC26533, Fusarium poae ATCC15654 independent packaging or mix respectively.
10. arbitrary described bacteria fermentation switchgrass, crop straws for producing cellulase and hemicellulase among the claim 1-9.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194407A (en) * | 2013-03-21 | 2013-07-10 | 江西农业大学 | Straw-decomposition composite microbial preparation and preparation method thereof |
| CN104004659A (en) * | 2014-04-02 | 2014-08-27 | 浙江大学 | Fusarium sporotrichioides and its application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560488A (en) * | 2009-05-27 | 2009-10-21 | 中国农业大学 | Enzyme and microbial inoculum for decomposing lignocellulose |
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2011
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Patent Citations (1)
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| CN101560488A (en) * | 2009-05-27 | 2009-10-21 | 中国农业大学 | Enzyme and microbial inoculum for decomposing lignocellulose |
Non-Patent Citations (1)
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| 贺军军，等: "甘蔗渣纤维素降解菌的筛选及鉴定", 《微生物学杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194407A (en) * | 2013-03-21 | 2013-07-10 | 江西农业大学 | Straw-decomposition composite microbial preparation and preparation method thereof |
| CN103194407B (en) * | 2013-03-21 | 2015-05-13 | 江西农业大学 | Straw-decomposition composite microbial preparation and preparation method thereof |
| CN104004659A (en) * | 2014-04-02 | 2014-08-27 | 浙江大学 | Fusarium sporotrichioides and its application |
| CN104004659B (en) * | 2014-04-02 | 2016-06-22 | 浙江大学 | A kind of Fusarium sporotrichioides and application thereof |
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| Publication number | Publication date |
|---|---|
| CN102161971B (en) | 2013-06-19 |
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