CN102160856A - Neglect-proof drinking immunization tablet for infectious bursal disease (IBD) and preparation method thereof - Google Patents
Neglect-proof drinking immunization tablet for infectious bursal disease (IBD) and preparation method thereof Download PDFInfo
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Abstract
The invention provides a neglect-proof drinking immunization tablet for infectious bursal disease (IBD), which comprises an antigen for IBD, a heat-resistant protective agent and an effervescent material, wherein the antigen for IBD and the heat-resistant protective agent are mixed to prepare a heat-resistant antigen freeze-dried particle, and then, the particle and the effervescent material are evenly mixed and pressed to obtain the neglect-proof drinking immunization tablet. Because a color indicator is added into the effervescent material, by checking whether chicken beak tips are dyed or not, the chicken which does not drink water can be easily found, and the chicken can drink water, thereby effectively avoiding the occurrence of an immunization neglect phenomenon; and a metal chelator, an immune potentiator and a heat-resistant protective agent are added, and a tablet is prepared, thus the vaccine is convenient to transport and store, and the storage time exceeds 24 months. When in use, the vaccine tablet provided by the invention only needs to be put into a proper amount of water for chicken flocks to drink to enable the chicken flocks to be immunized, thereby realizing the effective immunization of IBD of the chicken flocks, ensuring the immunization uniformity, preventing the immunization neglect, and enhancing the vaccine immunization effect. The tablet provided by the invention is most suitable for medium-and-large-sized chicken farms.
Description
Technical field
The present invention relates to a kind of vaccine novel form and preparation method, especially a kind ofly be applicable to drinking-water immunity, have indicative function and can activate body immune system rapidly, improve the infectious bursal disease Anti-leakage type drinking-water immune tablets of immune level.
Background technology
(Infectious bursal disease IBD) is a kind of strong hyperinfection disease of being attacked and being endangered young chicken in 3～8 ages in week by IBD virus (IBDV) to infectious bursal disease.The target organ of IBDV and target cell mainly are the bone-marrow-derived lymphocytes in central immune organ-fabricius bursa tissue of infringement chicken and cause B cell quantity minimizing, and then cause serious immunosuppressant.This sick coup injury is chicken infected certain mortality rate and mortality, and indirect harm is the chicken group of subclinical infection or anti-ly crosses the chicken group and secular, serious immunosuppressant can occur, cause the chicken group to other eqpidemic disease, susceptibility as bird flu, newcastle etc. increases, immunne response ability to vaccine reduces, immuning failure to deadly infectious disease causes enormous economic loss.
China's vaccine commonly used has two big classes at present: live vaccine and inactivated vaccine.The Seedling of living has three types, the one, attenuated vaccine, to fabricius bursa without any infringement, immunity back antibody produces late, it is lower to tire, and when nature runs into stronger IBDV, uses this class vaccine lower to the chicken protective rate, generally be used to not have the early immune of maternal antibody chickling, relatively poor to the chicken immune effect that maternal antibody is arranged; The 2nd, the mesogenic vaccine, there is the minor injury inoculation back to fabricius bursa, thisly disappears after being reflected at 10 days, to the protective rate height of the strong poison of serum I type, can be for the various chickling uses that maternal antibody be arranged; The 3rd, middle isogonic strong virus force type vaccine used all fabricius bursa to be caused serious harm before two ages in week, caused immunosuppressant, but did not influence the protection to IBD itself.Use the hen house of this class vaccine, but the vaccine virus long-term surviving infects next group children chickling in age, can cause serious immunosuppressant.In the clinical use, multiselect with the good mesogenic live vaccine of safety guaranteeing that fertility performance is stable, if but the maternal antibody level is still very high during immunity, then such chicken not only immune effect is poor, and can drag down its circulating antibody and cause morbidity several weeks of immunity back.So general poultry-farm is in order better to take into account immune efficacy and safety, general adopt mesogenic live vaccine head exempt from the back at interval two weeks again booster immunization once facts have proved that this is the selection of relatively optimizing.To produce antibody slow because inactivated vaccine is induced, and the immunoreation persistent period is shorter, and immune effect is lower than Seedling alive, therefore manyly clinically cooperates the Seedling of living to use with inactivated vaccine, is used for the booster immunization of kind of chicken, makes the higher maternal antibody of the institute's chicken that farrows acquisition.
The infectious bursal disease live-vaccine heat stability is poor; according to " People's Republic of China's veterinary biologics rules "; the protective agent that infectious bursal disease mesogenic live vaccine uses is 5% sucrose skim milk protective agent; under 2～8 ℃ of holding conditions; the vaccine shelf-life only is 12 months; this just requires from product rolls off the production line the whole storage and transportation that inoculation uses; all to remain on " cold chain " environment below-15 ℃; this brings many difficulties for the storage and the transportation of vaccine; in addition; because the restriction of refrigerated condition; the vaccine end user also is difficult to guarantee to make vaccine be in environment below-15 ℃ before use; therefore; in the transportation and storage of vaccine; usually descend, even, make plant (family) suffer enormous economic loss because of vaccine failure causes immuning failure owing to the cold preservation reason causes vaccine potency.The immunity success or not, except the program of selecting effective vaccine, science for use, immune quality also is one of key factor.General drinking-water immunity and the two kinds of approach of drip immunity of adopting of IBD live vaccine immunity.The drip immunity is to adopt by plumage to drip the method for clothes to chicken immune, though immune effect is better than the drinking-water immunity, but very trouble operates, the drinking-water immunity is then more time saving and energy saving, present many chicken houses for convenience, the general drinking-water that adopts is immune, especially during secondary immunity, but in order to guarantee vaccine activity, must guarantee in 0.5 ~ 1 hour, vaccine have been drunk during the drinking-water immunity, all drink vaccine water owing to can't guarantee all chickens of chicken group, also can't distinguish the chicken of drinking vaccine and not drinking vaccine, therefore immune effect is uncertain, very easily occurs leaking exempting from phenomenon, causes the drinking-water vaccinated flock still to have morbidity in various degree.
Summary of the invention
The objective of the invention is in order to overcome the weak point of above-mentioned existing infectious bursal disease live-vaccine drinking-water immunity, provide a kind of simple to operate, easy to use, be convenient to transportation and storage, be applicable to drinking-water immunity and can prevent effectively that leak-stopping exempts from phenomenon and can activate body immune system rapidly, the infectious bursal disease Anti-leakage type that the improves immune level immune tablets of drinking water.
Another object of the present invention is the preparation method that a kind of suitable large-scale production is provided for above-mentioned infectious bursal disease Anti-leakage type drinking-water immune tablets.
Infectious bursal disease Anti-leakage type drinking-water immune tablets of the present invention, comprise infectious bursal disease antigen, also comprise heat resisting protective and effervescent materials, after described infectious bursal disease antigen and heat resisting protective are hybridly prepared into heat stable antigen lyophilizing granule, again with the effervescent materials mixing after tabletting form; Configuration proportion in the described heat stable antigen lyophilizing granule is that every 100g infectious bursal disease antigen mixes with 100 ~ 150ml heat resisting protective, and the configuration proportion in the tablet is that every 200g heat stable antigen lyophilizing granule mixes with 100 ~ 300g effervescent materials; Wherein, described heat resisting protective is made up of gelatin, sucrose, dextrin, casein hydrolysis, trehalose, sorbitol, L-sodium glutamate, L-cysteine and distilled water, and its configuration proportion is to add gelatin 1 ~ 2g, sucrose 5 ~ 6g, dextrin 6 ~ 10g, casein hydrolysis 0.5 ~ 1g, trehalose 3 ~ 5g, sorbitol 2g, L-sodium glutamate 1 ~ 2g, L-cysteine 0.5 ~ 1g in every 200ml distilled water; Described effervescent materials is by citric acid or citric acid, sodium bicarbonate, sucrose or xylitol or lactose, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt, light blue indicator or phenol red indicator, levamisole or cimetidine or neo-houttuyninum are formed, its preparation mass parts is: citric acid or citric acid are 10 ~ 30 parts, sodium bicarbonate is 10 ~ 30 parts, sucrose or xylitol or lactose are 20 ~ 40 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are 20 ~ 30 parts, and light blue indicator or phenol red indicator are 2 ~ 6 parts, and levamisole or cimetidine or neo-houttuyninum are 0.5 ~ 1 part.
The preparation method of infectious bursal disease Anti-leakage type drinking-water immune tablets of the present invention mainly comprises: preparation heat resisting protective, preparation infectious bursal disease antigen, preparation heat stable antigen lyophilizing granule, preparation effervescent materials, mixed pressuring plate; Its concrete steps are as follows:
1) preparation heat resisting protective:
A) be placed on 116 ℃ of sterilization 30min by adding gelatin 1 ~ 2g, sucrose 5 ~ 6g, dextrin 6 ~ 10g mixing in every 100ml distilled water earlier, it is standby to get A side;
B) again by after adding casein hydrolysis 0.5 ~ 1g, trehalose 3 ~ 5g, sorbitol 2g, L-sodium glutamate 1 ~ 2g, L-cysteine 0.5 ~ 1g mixing in every 100ml distilled water, with the membrane filtration degerming of aperture 0.22 μ m, it is standby to get B side;
C) by volume part be that the mixed of 1 ︰ 1 is even then with above-mentioned A side and B side, heat resisting protective is standby;
2) preparation infectious bursal disease antigen:
The infectious bursal disease mesogenic B87 strain that will provide by China Veterinery Drug Inspection Office, conventional method according to " infectious bursal disease mesogenic live vaccine is made and the inspection procedure " regulation in " People's Republic of China's veterinary biologics rules ", make infectious bursal disease antigen, in freezing preservation below-10 ℃, standby;
3) preparation heat stable antigen lyophilizing granule:
In the ratio of the heat resisting protective of every 100g infectious bursal disease antigen and 100 ~ 150ml prepare and mix homogeneously, the pallet of packing into, lyophilizing after be broken into graininess with ball mill, heat stable antigen lyophilizing granule, in freezing preservation below-10 ℃, standby;
4) preparation effervescent materials:
A) preparation acid particles: mass parts is respectively: it is that 10 ~ 20 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are that 20 ~ 30 parts, light blue indicator or phenol red indicator are 1 ~ 3 part and fully mix with sucrose or xylitol or lactose that citric acid or citric acid are 10 ~ 30 parts, after 40 ~ 65 ℃ of oven dry, cross 8 ~ 12 purpose sieve series grains, get acid particles, standby;
B) preparation alkali grain: mass parts is respectively: it is that 10 ~ 20 parts, light blue indicator or phenol red indicator are 1 ~ 3 part and fully mix with sucrose or xylitol or lactose that sodium bicarbonate is 10 ~ 30 parts, after 40 ~ 65 ℃ of oven dry, cross 8 ~ 12 purpose sieve series grains, get alkali grain, standby;
C) before tabletting be that 0.5 ~ 1 levamisole or cimetidine or neo-houttuyninum fully mix with above-mentioned standby acid particles and alkali grain and mass parts, effervescent materials;
5) mixed pressuring plate:
Will by the mixed of every 200g heat stable antigen lyophilizing granule and 100 ~ 300g effervescent materials and 2 ~ 5g magnesium stearate evenly after, be pressed into tablet with tablet machine, infectious bursal disease Anti-leakage type drinking-water immune tablets.
Infectious bursal disease Anti-leakage type drinking-water immune tablets of the present invention, owing in effervescent materials, added color indicator light blue indicator or phenol red indicator, during use, by checking dyeing of beak point and undyed situation, be easy to find and the chicken that will not drink water is mended drink and can effectively avoid Lou exempting from the phenomenon appearance; Added metal-chelator disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt, effectively the heavy metal ion in the chelating water overcomes the adverse effect of heavy metal ion to vaccine; Also add immunostimulant levamisole or cimetidine or neo-houttuyninum, can strengthen immune effect of vaccine; Also added the heat-resisting protective composition in the component, and made Tabules, made vaccine be convenient to transportation and storage, storage time was above 24 months.During use, only need in the water that vaccine tablet input of the present invention is an amount of, for the chicken group immunity of drinking water, can realize making the effective immunity of chicken group to infectious bursal disease, not only can guarantee the immune uniformity, anti-leak-stopping is exempted from, can also strengthen immune effect of vaccine, the most suitable in, large-scale chicken farm uses.
The specific embodiment
Infectious bursal disease Anti-leakage type drinking-water immune tablets of the present invention; mainly form by infectious bursal disease antigen, heat resisting protective and effervescent materials; its preparation method mainly comprises: steps such as preparation heat resisting protective, preparation infectious bursal disease antigen, preparation heat stable antigen lyophilizing granule, preparation effervescent materials and mixed pressuring plate now are further described as follows in conjunction with specific embodiments to the present invention:
1) preparation heat resisting protective:
This heat resisting protective is formed by A side and the B side's uniform mixing prepared respectively in advance,
The compound method of A side wherein is:
High temperature sterilize behind adding gelatin 1g, sucrose 5g, the dextrin 6g mixing in every 100ml distilled water;
Or in every 100ml distilled water, add high temperature sterilize behind gelatin 1g, sucrose 6g, the dextrin 10g mixing;
Or in every 100ml distilled water, add high temperature sterilize behind gelatin 2g, sucrose 5g, the dextrin 6g mixing;
Or in every 100ml distilled water, add high temperature sterilize behind gelatin 2g, sucrose 6g, the dextrin 10g mixing;
Or in every 100ml distilled water, add high temperature sterilize behind gelatin 2g, sucrose 5g, the dextrin 10g mixing;
Or in every 100ml distilled water, add high temperature sterilize behind gelatin 2g, sucrose 6g, the dextrin 6g mixing;
Or in every 100ml distilled water, add high temperature sterilize behind gelatin 1g, sucrose 5g, the dextrin 10g mixing;
Or in every 100ml distilled water, add high temperature sterilize behind gelatin 1g, sucrose 6g, the dextrin 6g mixing;
Or in every 100ml distilled water, add high temperature sterilize behind gelatin 1.5g, sucrose 5.5g, the dextrin 8g mixing;
That is: can add high temperature sterilize behind gelatin 1 ~ 2g, sucrose 5 ~ 6g, the dextrin 6 ~ 10g mixing in every 100ml distilled water, the temperature of high temperature sterilize is elected 116 ℃ as, and sterilization time is elected 30min as; , it is standby all can to get A side;
The compound method of B side wherein is:
In every 100ml distilled water, add casein hydrolysis 0.5g, trehalose 3g, sorbitol 2g, L-sodium glutamate 1g, the degerming of L-cysteine 0.5g mixing after-filtration;
Or adding casein hydrolysis 0.5g, trehalose 5g, sorbitol 2g, L-sodium glutamate 2g, the degerming of L-cysteine 1g mixing after-filtration in every 100ml distilled water;
Or adding casein hydrolysis 0.5g, trehalose 4g, sorbitol 2g, L-sodium glutamate 2g, the degerming of L-cysteine 1g mixing after-filtration in every 100ml distilled water;
Or adding casein hydrolysis 1g, trehalose 3g, sorbitol 2g, L-sodium glutamate 1g, the degerming of L-cysteine 0.5g mixing after-filtration in every 100ml distilled water;
Or adding casein hydrolysis 1g, trehalose 5g, sorbitol 2g, L-sodium glutamate 2g, the degerming of L-cysteine 1g mixing after-filtration in every 100ml distilled water;
That is: can in every 100ml distilled water, add casein hydrolysis 0.5 ~ 1g, trehalose 3 ~ 5g, sorbitol 2g, L-sodium glutamate 1 ~ 2g, the degerming of L-cysteine 0.5 ~ 1g mixing after-filtration, the filter membrane of filtration sterilization is selected the filter membrane of aperture 0.22 μ m for use, and it is standby all can to get B side;
The mixed that with above-mentioned A side and B side by volume part is 1 ︰ 1 then is even, heat resisting protective is standby;
2) preparation infectious bursal disease antigen:
The infectious bursal disease mesogenic B87 strain that will provide by China Veterinery Drug Inspection Office, conventional method according to " infectious bursal disease mesogenic live vaccine is made and the inspection procedure " regulation in " People's Republic of China's veterinary biologics rules ", make infectious bursal disease antigen, in freezing preservation below-10 ℃, standby;
3) preparation heat stable antigen lyophilizing granule:
Ratio in the heat resisting protective of every 100g infectious bursal disease antigen and 100ml;
Or in the ratio of the heat resisting protective of every 100g infectious bursal disease antigen and 100 ~ 125ml;
Or in the ratio of the heat resisting protective of every 100g infectious bursal disease antigen and 100 ~ 150ml;
That is: prepare in the ratio of the heat resisting protective of every 100g infectious bursal disease antigen and 100 ~ 150ml and mix homogeneously, the pallet of packing into, lyophilizing after be broken into graininess with ball mill, get heat stable antigen lyophilizing granule, in freezing preservation below-10 ℃, standby;
4) effervescent materials preparation:
This effervescent materials is mixed by the acid particles and the alkali grain of levamisole or cimetidine or neo-houttuyninum and preparation in advance, specifically describes as follows:
A) preparation acid particles:
Mass parts is respectively: citric acid or citric acid are that 10 parts, sucrose or xylitol or lactose are that 10 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are that 20 parts, light blue indicator or phenol red indicator are 1 part;
Or mass parts is respectively: citric acid or citric acid are that 10 parts, sucrose or xylitol or lactose are that 10 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are that 30 parts, light blue indicator or phenol red indicator are 3 parts;
Or mass parts is respectively: citric acid or citric acid are that 10 parts, sucrose or xylitol or lactose are that 20 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are that 30 parts, light blue indicator or phenol red indicator are 2 parts;
Or mass parts is respectively: citric acid or citric acid are that 30 parts, sucrose or xylitol or lactose are that 10 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are that 20 parts, light blue indicator or phenol red indicator are 1 part;
Or mass parts is respectively: citric acid or citric acid are that 30 parts, sucrose or xylitol or lactose are that 20 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are that 30 parts, light blue indicator or phenol red indicator are 3 parts;
Or mass parts is respectively: citric acid or citric acid are that 20 parts, sucrose or xylitol or lactose are that 15 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are that 25 parts, light blue indicator or phenol red indicator are 2 parts;
That is: mass parts is respectively: citric acid or citric acid are that 10 ~ 30 parts, sucrose or xylitol or lactose are that 10 ~ 20 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are that 20 ~ 30 parts, light blue indicator or phenol red indicator are 1 ~ 3 part and fully mix, after 40 ~ 65 ℃ of oven dry, cross 8 ~ 12 purpose sieve series grains, all can make acid particles, standby;
B) preparation alkali grain:
Mass parts is respectively: sodium bicarbonate is that 10 parts, sucrose or xylitol or lactose are that 10 parts, light blue indicator or phenol red indicator are 1 part;
Or mass parts is respectively: sodium bicarbonate is that 10 parts, sucrose or xylitol or lactose are that 20 parts, light blue indicator or phenol red indicator are 3 parts;
Or mass parts is respectively: sodium bicarbonate is that 30 parts, sucrose or xylitol or lactose are that 10 parts, light blue indicator or phenol red indicator are 2 parts;
Or mass parts is respectively: sodium bicarbonate is that 30 parts, sucrose or xylitol or lactose are that 20 parts, light blue indicator or phenol red indicator are 1 part;
Or mass parts is respectively: it is that 15 parts, light blue indicator or phenol red indicator are 3 parts with sucrose or xylitol or lactose that sodium bicarbonate is 20 parts;
That is: mass parts is respectively: sodium bicarbonate is that 10 ~ 30 parts, sucrose or xylitol or lactose are that 10 ~ 20 parts, light blue indicator or phenol red indicator are 1 ~ 3 part and fully mix, after 40 ~ 65 ℃ of oven dry, cross 8 ~ 12 purpose sieve series grains, all can make alkali grain, standby;
C) before tabletting be that 0.5 ~ 1 levamisole or cimetidine or neo-houttuyninum fully mix with above-mentioned standby acid particles and alkali grain and mass parts, effervescent materials;
5) mixed pressuring plate:
Ratio in every 200g heat stable antigen lyophilizing granule and 100g effervescent materials and 2g magnesium stearate;
Or in the ratio of every 200g heat stable antigen lyophilizing granule and 100g effervescent materials and 5g magnesium stearate;
Or in the ratio of every 200g heat stable antigen lyophilizing granule and 200g effervescent materials and 2g magnesium stearate;
Or in the ratio of every 200g heat stable antigen lyophilizing granule and 200g effervescent materials and 5g magnesium stearate;
Or in the ratio of every 200g heat stable antigen lyophilizing granule and 300g effervescent materials and 3g magnesium stearate;
Or in the ratio of every 200g heat stable antigen lyophilizing granule and 300g effervescent materials and 5g magnesium stearate;
That is: by the mixed of every 200g heat stable antigen lyophilizing granule and 100 ~ 300g effervescent materials and 2 ~ 5g magnesium stearate evenly after, be pressed into tablet with tablet machine, all can make the infectious bursal disease Anti-leakage type of the present invention immune tablets of drinking water.
In order to verify that vaccine of the present invention can prevent that leak-stopping exempts from and can strengthen chicken body immunne response, improve immune effect of vaccine, the spy does following experiment.
Infectious bursal disease Anti-leakage type drinking-water immune tablets of the present invention is to indicating effect test and the immune efficacy laboratory report of immune chicken
1, experiment material
1.1 experimental vaccine: infectious bursal disease Anti-leakage type drinking-water immune tablets (B87 strain) of the present invention.
1.2 control vaccine: infectious bursal disease heat resisting protective live vaccine (B87 strain), purchase in market.
1.3 SPF chickling: the SPF hatching egg is provided biological pharmaceutical factory, Qianyuanhao Biological Co., Ltd. Nanjing and in its hatching house's hatching, chickling is raised in isolator.
1.4 virulent strain BC6-85: provide by China Veterinery Drug Inspection Office.
2, method
2.1 14 150 of age in days SPF chickling are divided into 5 groups, every group each 30.First group is the immune infectious bursal disease Anti-leakage type drinking-water immune tablets of the present invention of drinking-water; second group is the immune conventional infectious bursal disease heat resisting protective live vaccine of drinking-water; the 3rd group is the conventional infectious bursal disease heat resisting protective live vaccine of drip immunity; every chicken 1 plumage part; the 4th group is the not immune matched group of counteracting toxic substances, and the 5th group is the blank group.Under identical raising condition, separately raise for every group, and do not have painted chicken separately to raise first group of mediproboscis point, be labeled as the 6th group.Get 10 respectively through 3 weeks, 6 the first five group of week back, get 1/2nd total (at least 3) of this group for the 6th group, detection IBD SN antibody, calculating mean value, and with the strong malicious BC6-85 strain eye dripping 0.1ml attack of 10 minimal infecting dose (MID)s.
2.2 serum neutralization (SN) test fixed virus dilute serum, cell culture method is undertaken by " People's Republic of China's veterinary biologics rules " (2000 editions) 446-447 page or leaf.
2.3 test chicken is observed and to be observed 96 hours behind the counteracting toxic substances and every chicken is cutd open inspection, observes the fabricius bursa pathological changes, calculates protective rate.
3 results
The antibody horizontal and the counteracting toxic substances protection situation in infectious bursal disease live-vaccine 3 weeks, 6 weeks behind immune 14 age in days SPF chickens see Table 1.
Table 1 infectious bursal disease live-vaccine immune efficacy and immune period test
Annotate: 1, the fabricius bursa pathological changes shows as the serious atrophy of fabricius bursa, is significantly less than the fabricius bursa of blank group;
2, "-" represents that this Xiang Wei does.
4 brief summaries
4.1 after the immunity of infectious bursal disease Anti-leakage type drinking-water immune tablets drinking-water; with dyeing of beak point and undyed separate detection; the dyeing chicken of finding Band 1 obtains effective immunoprotection; and the chicken of being unstained of the 6th group does not obtain immunoprotection; the result shows; can prevent when this vaccine is used to drink water immunity that leak-stopping exempts from phenomenon, all once guarantee immunity.
Immune 1 plumage part infectious bursal disease Anti-leakage type drinking-water immune tablets 4.2 the Band 1 chicken drinks water when 14 ages in days; SN average antibody level reaches 1:640 after 6 weeks; protective rate to the strong malicious BC6-85 strain of IBDV can reach more than 90%; with the testing result basically identical of the 3rd group, and be better than second group of detection effect with the immunity of conventional heat resisting protective live vaccine drinking-water with the immunity of conventional heat resisting protective live vaccine drip.The result shows that the effective ingredient in the infectious bursal disease Anti-leakage type drinking-water immune tablets of the present invention can strengthen chicken body immunne response, improves immune effect of vaccine.
Claims (2)
1. infectious bursal disease Anti-leakage type drinking-water immune tablets, comprise infectious bursal disease antigen, it is characterized in that also comprising heat resisting protective and effervescent materials, after described infectious bursal disease antigen and heat resisting protective are hybridly prepared into heat stable antigen lyophilizing granule, again with the effervescent materials mixing after tabletting form; Configuration proportion in the described heat stable antigen lyophilizing granule is that every 100g infectious bursal disease antigen mixes with 100 ~ 150ml heat resisting protective, and the configuration proportion in the tablet is that every 200g heat stable antigen lyophilizing granule mixes with 100 ~ 300g effervescent materials; Wherein, described heat resisting protective is made up of gelatin, sucrose, dextrin, casein hydrolysis, trehalose, sorbitol, L-sodium glutamate, L-cysteine and distilled water, and its configuration proportion is to add gelatin 1 ~ 2g, sucrose 5 ~ 6g, dextrin 6 ~ 10g, casein hydrolysis 0.5 ~ 1g, trehalose 3 ~ 5g, sorbitol 2g, L-sodium glutamate 1 ~ 2g, L-cysteine 0.5 ~ 1g in every 200ml distilled water; Described effervescent materials is by citric acid or citric acid, sodium bicarbonate, sucrose or xylitol or lactose, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt, light blue indicator or phenol red indicator, levamisole or cimetidine or neo-houttuyninum are formed, its preparation mass parts is: citric acid or citric acid are 10 ~ 30 parts, sodium bicarbonate is 10 ~ 30 parts, sucrose or xylitol or lactose are 20 ~ 40 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are 20 ~ 30 parts, and light blue indicator or phenol red indicator are 2 ~ 6 parts, and levamisole or cimetidine or neo-houttuyninum are 0.5 ~ 1 part.
2. the preparation method of the drinking-water immune tablets of right 1 is characterized in that comprising: preparation heat resisting protective, preparation infectious bursal disease antigen, preparation heat stable antigen lyophilizing granule, preparation effervescent materials, mixed pressuring plate; Its concrete steps are as follows:
1) preparation heat resisting protective:
A) be placed on 116 ℃ of sterilization 30min by adding gelatin 1 ~ 2g, sucrose 5 ~ 6g, dextrin 6 ~ 10g mixing in every 100ml distilled water earlier, it is standby to get A side;
B) again by after adding casein hydrolysis 0.5 ~ 1g, trehalose 3 ~ 5g, sorbitol 2g, L-sodium glutamate 1 ~ 2g, L-cysteine 0.5 ~ 1g mixing in every 100ml distilled water, with the membrane filtration degerming of aperture 0.22 μ m, it is standby to get B side;
C) by volume part be that the mixed of 1 ︰ 1 is even then with above-mentioned A side and B side, heat resisting protective is standby;
2) preparation infectious bursal disease antigen:
The infectious bursal disease mesogenic B87 strain that will provide by China Veterinery Drug Inspection Office, conventional method according to " infectious bursal disease mesogenic live vaccine is made and the inspection procedure " regulation in " People's Republic of China's veterinary biologics rules ", make infectious bursal disease antigen, in freezing preservation below-10 ℃, standby;
3) preparation heat stable antigen lyophilizing granule:
In the ratio of the heat resisting protective of every 100g infectious bursal disease antigen and 100 ~ 150ml prepare and mix homogeneously, the pallet of packing into, lyophilizing after be broken into graininess with ball mill, heat stable antigen lyophilizing granule, in freezing preservation below-10 ℃, standby;
4) preparation effervescent materials:
A) preparation acid particles: mass parts is respectively: it is that 10 ~ 20 parts, disodiumedetate or ethylenediamine tetraacetic acid,dipotassium salt are that 20 ~ 30 parts, light blue indicator or phenol red indicator are 1 ~ 3 part and fully mix with sucrose or xylitol or lactose that citric acid or citric acid are 10 ~ 30 parts, after 40 ~ 65 ℃ of oven dry, cross 8 ~ 12 purpose sieve series grains, get acid particles, standby;
B) preparation alkali grain: mass parts is respectively: it is that 10 ~ 20 parts, light blue indicator or phenol red indicator are 1 ~ 3 part and fully mix with sucrose or xylitol or lactose that sodium bicarbonate is 10 ~ 30 parts, after 40 ~ 65 ℃ of oven dry, cross 8 ~ 12 purpose sieve series grains, get alkali grain, standby;
C) before tabletting be that 0.5 ~ 1 levamisole or cimetidine or neo-houttuyninum fully mix with above-mentioned standby acid particles and alkali grain and mass parts, effervescent materials;
5) mixed pressuring plate:
Will by the mixed of every 200g heat stable antigen lyophilizing granule and 100 ~ 300g effervescent materials and 2 ~ 5g magnesium stearate evenly after, be pressed into tablet with tablet machine, infectious bursal disease Anti-leakage type drinking-water immune tablets.
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| WO2016057978A1 (en) * | 2014-10-10 | 2016-04-14 | Merial Inc. | Reduced foaming vaccine compositions |
| EP3323298A1 (en) * | 2015-12-23 | 2018-05-23 | Brauns-Heitmann GmbH & Co. KG | Powdery composition for coloring eggs |
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| CN1785163A (en) * | 2005-11-22 | 2006-06-14 | 瑞普（保定）生物药业有限公司 | Immune effervescent tablets for drinking water and its prepn. method |
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| CN1528453A (en) * | 2001-12-21 | 2004-09-15 | 卫广森 | Heat resisting lyophilized protectant for chicken infections Fabianite bursa hypotpxicity (B87) lyophilized vaccine and preparing process thereof |
| CN1785163A (en) * | 2005-11-22 | 2006-06-14 | 瑞普（保定）生物药业有限公司 | Immune effervescent tablets for drinking water and its prepn. method |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016057978A1 (en) * | 2014-10-10 | 2016-04-14 | Merial Inc. | Reduced foaming vaccine compositions |
| KR20170066532A (en) * | 2014-10-10 | 2017-06-14 | 메리얼 인코포레이티드 | Reduced foaming vaccine compositions |
| CN107106667A (en) * | 2014-10-10 | 2017-08-29 | 梅里亚股份有限公司 | The reduced vaccine combination of foaming |
| JP2017534609A (en) * | 2014-10-10 | 2017-11-24 | メリアル インコーポレイテッド | Foam reduction vaccine composition |
| AU2015330716B2 (en) * | 2014-10-10 | 2018-12-06 | Abic Biological Laboratories Ltd | Reduced foaming vaccine compositions |
| US10213509B2 (en) | 2014-10-10 | 2019-02-26 | Merial Inc. | Reduced foaming vaccine compositions |
| US10610596B2 (en) | 2014-10-10 | 2020-04-07 | Abic Biological Laboratories Ltd. | Methods of making solid vaccine compositions having reduced foaming |
| RU2731534C2 (en) * | 2014-10-10 | 2020-09-04 | Эбик Байолоджикал Лабораториз Лтд. | Vaccines with reduced foam formation |
| CN107106667B (en) * | 2014-10-10 | 2022-03-15 | 勃林格殷格翰动物保健美国公司 | Vaccine compositions with reduced foaming |
| KR102652509B1 (en) | 2014-10-10 | 2024-03-28 | 아빅 바이오로지칼 라보라토리즈 리미티드 | Reduced foaming vaccine compositions |
| EP3323298A1 (en) * | 2015-12-23 | 2018-05-23 | Brauns-Heitmann GmbH & Co. KG | Powdery composition for coloring eggs |
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