CN102154099B

CN102154099B - Miniature thermal cycle control system for polymerase chain reaction (PCR) biochip

Info

Publication number: CN102154099B
Application number: CN 201010603514
Authority: CN
Inventors: 赵玉龙; 刘亦敏; 聂金泉; 彭年才; 蒋庄德
Original assignee: Xian Jiaotong University
Current assignee: Xian Jiaotong University
Priority date: 2010-12-20
Filing date: 2010-12-20
Publication date: 2013-02-27
Anticipated expiration: 2030-12-20
Also published as: CN102154099A

Abstract

The invention discloses a miniature thermal cycle control system for a polymerase chain reaction (PCR) biochip. The system comprises a microprocessor and is characterized in that the microprocessor and other modules are respectively connected with a voltage conversion module; a temperature data acquisition and amplification module, a key control circuit, a signal output powder module, a liquid crystal display module, a joint test action group (JTAG) simulation module are respectively connected with the microprocessor; the temperature data acquisition and amplification module is connected with a Pt temperature sensor; the signal output powder module is connected with a semiconductor heater; a liquid in a micro-reactor chamber is heated through a semiconductor on a PCR miniature machinery integrated chip, and the Pt temperature-measuring sensor converts a measured temperature signal into a weak voltage signal through a bridge circuit, amplifies the weak voltage signal and then transmits the amplified weak voltage signal to the microprocessor; and the microprocessor controls the on-off of the semiconductor heater according to the amplified weak voltage signal, thereby achieving the purpose of controlling the liquid heating time and regulating the heating temperature. The system has the advantages of small volume, low power consumption, simple hardware structure, short circulation time and high control precise, and is convenient for supplying electricity.

Description

A kind of miniature thermal cycling Controlling System of PCR biochip

Technical field

The present invention relates to biochip Controlling System technical field, particularly a kind of miniature thermal cycling Controlling System of PCR biochip.

Background technology

Polymerase chain reaction (PCR) is a kind of method of the synthetic specific DNA fragment of external enzymatic, the method is because of its characteristics such as easy, quick, efficient, become rapidly the most common technique of present DNA amplification, have a wide range of applications in fields such as Aeronautics and Astronautics, military affairs, medical science, life sciences.The PCR process is looped by a few step reaction composition one-period such as high-temperature denatured, low-temperature annealing and thermophilic extensions, temperature of reaction is required very high, and it is directly connected to the success or failure of amplification.Therefore, the thermal cycling control system design of PCR biochip is very important.At present, the PCR biochip has been realized microminiaturization, but its thermal cycling Controlling System still is the Controlling System of normal PCR amplification instrument, exist the shortcomings such as volume is large, power consumption is high, hardware configuration is complicated, temperature-controlled precision is poor, cycling time is long, also inconvenient with being connected of miniature pcr chip, seriously restricted the integrated, microminiaturized of PCR biochip system.In addition, existing PCR thermal cycling Controlling System all adopts the external source power supply, has directly limited the exploitation of portable PC R equipment.

Summary of the invention

In order to overcome the shortcoming of above-mentioned prior art, the object of the present invention is to provide a kind of miniature thermal cycling Controlling System of PCR biochip, have that volume is little, low in energy consumption, hardware configuration is simple, cycling time is short, control accuracy is high and power easily advantage.

For achieving the above object, the present invention takes following technical scheme:

A kind of miniature thermal cycling Controlling System of PCR biochip, comprise microprocessor 1, the operating voltage input terminus of microprocessor 1 links to each other with the first output terminal of voltage transformation module 2, temperature data acquisition links to each other with the operating voltage input terminus of amplification module 3 and the second output terminal of voltage transformation module 2, the operating voltage input terminus of output power signal module 4 links to each other with the 3rd output terminal of voltage transformation module 2, the operating voltage input terminus of LCD MODULE 6 links to each other with the 4th output terminal of voltage transformation module 2, the operating voltage input terminus of JTAG emulation module 8 links to each other with the 5th output terminal of voltage transformation module 2, temperature data acquisition links to each other with the output terminal of amplification module 3 and the first input end of microprocessor 1, the output terminal of key control circuit 7 links to each other with the second input terminus of microprocessor 1, the first output terminal of microprocessor 1 links to each other with the input terminus of output power signal module 4, the second output terminal of microprocessor 1 links to each other with the input terminus of LCD MODULE 6, the 3rd output terminal of microprocessor 1 links to each other with the input terminus of JTAG emulation module 8, the input terminus of temperature data acquisition and amplification module 3 links to each other with Pt temperature sensor 10 on the PCR micromechanics chip 9 by the first interface of terminal block 5, second interface of the output terminal of output power signal module 4 by terminal block 5 links to each other with semiconductor heat booster 11 on the PCR micromechanics chip 9, integrative installation technology is adopted in being connected of terminal block 5 and PCR micromechanics chip 9, and the mode by dual-in-line is connected to the pin of Pt temperature sensor 10 and semiconductor heat booster 11 on the corresponding via hole of system circuit board.

The microprocessor 1 of the miniature thermal cycling Controlling System of described PCR biochip comprises cpu chip A1, the 1f pin of cpu chip A1, the 64f pin respectively with an end of capacitor C 1, one end of capacitor C 2 links to each other, the other end of capacitor C 1, the other end ground connection GND of capacitor C 2, the 7f pin of cpu chip A1 and the positive pole of chemical capacitor C5, one end of capacitor C 6 links to each other, the negative pole of chemical capacitor C5, the other end ground connection GND of capacitor C 6, the 8f pin of cpu chip A1 links to each other with the end of quartz oscillator Y1, the other end of quartz oscillator Y1 links to each other with the 9f pin of cpu chip A1, the 11f pin ground connection GND of cpu chip A1, the 52f pin of cpu chip A1, the 53f pin links to each other with the two ends of quartz oscillator Y2 respectively, the two ends of quartz oscillator Y2 more respectively with an end of capacitor C 4, one end of capacitor C 3 links to each other, the other end of capacitor C 4, the other end ground connection GND of capacitor C 3, the 62f pin of cpu chip A1,63f pin ground connection GND, the 1f pin of cpu chip A1, the 64f pin meets power supply VCC3.3.

The voltage transformation module 2 of the miniature thermal cycling Controlling System of described PCR biochip comprises voltage transitions chip and two parts of double-row needle socket, the input terminus of double-row needle socket J4 links to each other with battery, the output terminal of double-row needle socket J4 links to each other with the input terminus of voltage transitions chip U1, the output terminal of voltage transitions chip U1 links to each other with the input terminus of double-row needle socket J5, the output terminal of double-row needle socket J5 links to each other with the input terminus of voltage transitions chip U2, the 1a pin ground connection GND of voltage transitions chip U1, the 2a pin of voltage transitions chip U1 connects an end of capacitor C 9, link to each other with the 3c pin of voltage transitions chip U2 after the positive pole of chemical capacitor C10, the other end of capacitor C 9, the minus earth GND of chemical capacitor C10, the 3a pin of voltage transitions chip U1 connects the positive pole of chemical capacitor C7, one end of capacitor C 8, the positive pole of chemical capacitor C7, one end of capacitor C 8 connects the 2b pin of double-row needle socket J4 again, the negative pole of chemical capacitor C7, the other end ground connection GND of capacitor C 8, the 1b pin ground connection GND of double-row needle socket J4, the 1c pin ground connection GND of voltage transitions chip U2, the 2c pin of voltage transitions chip U2 connects an end of capacitor C 13, meet power supply VCC3.3 after the positive pole of chemical capacitor C14, the other end of capacitor C 13, the minus earth GND of chemical capacitor C14, the 3c pin of voltage transitions chip U2 connects the positive pole of chemical capacitor C11, one end of capacitor C 12, the positive pole of chemical capacitor C11, one end of capacitor C 12 connects the 2d pin of double-row needle socket J5 again, the negative pole of chemical capacitor C11, the other end ground connection GND of capacitor C 12, the 1d pin ground connection GND of double-row needle socket J5.

Temperature data acquisition and the amplification module 3 of the miniature thermal cycling Controlling System of described PCR biochip comprise the instrument amplification chip, bridge diagram and double-row needle socket three parts, the input terminus of double-row needle socket J2 links to each other with Pt temperature sensor 10 on the PCR biochip 9, the output terminal of double-row needle socket J2 links to each other with the input terminus of bridge diagram, the output terminal of bridge diagram links to each other with the input terminus of instrument amplification chip U3, the output terminal of instrument amplification chip U3 links to each other with the input terminus of microprocessor 1, the 1e pin of instrument amplification chip U3 links to each other with the 2l pin with the 1l pin of potentiometer RP2, the 3l pin of potentiometer RP2 links to each other with the 8e pin of instrument amplification chip U3, the 2e pin of instrument amplification chip U3 links to each other with an end of resistance R 2, the other end of resistance R 2 links to each other with the 2g pin of double-row needle socket J2, the 2e pin of instrument amplification chip U3 links to each other with an end of resistance R 3, the other end ground connection GND of resistance R 3, the 3e pin of instrument amplification chip U3 links to each other with the 1g pin of double-row needle socket J2, the 1g pin of double-row needle socket J2 links to each other with an end of resistance R 4, the other end ground connection GND of resistance R 4, the 4e pin of instrument amplification chip U3,5e pin ground connection GND, the 7e pin of instrument amplification chip U3 meets power supply VCC5.0, and the 6e pin of instrument amplification chip U3 links to each other with the 59f pin of the A1 chip of microprocessor 1.

The output power signal module 4 of the miniature thermal cycling Controlling System of described PCR biochip comprises photoelectric isolated chip, triode and double-row needle socket three parts, photoelectric isolated chip U4 links to each other with semiconductor heat booster 11 on the PCR micromechanics chip 9 by triode, microprocessor 1 is controlled the driving of power on the semiconductor heat booster 11 by output PWM ripple, the 1h pin of photoelectric isolated chip U4 links to each other with an end of resistance R 5, the other end of resistance R 5 links to each other with power supply VCC3.3, the 2h pin of photoelectric isolated chip U4 links to each other with the collector electrode c2 pin of triode Q2, the base stage b2 pin of triode Q2 links to each other with an end of resistance R 6, the other end of resistance R 6 links to each other with the 36f pin of the A1 chip of microprocessor 1, the emitter e 2 pin ground connection GND of triode Q2, the 6h pin of photoelectric isolated chip U4 links to each other with the collector electrode c1 pin of triode Q1, the collector electrode c1 pin of triode Q1 links to each other with power supply VCC15.0, the 4h pin of photoelectric isolated chip U4 links to each other with an end of resistance R 7, the other end of resistance R 7 links to each other with the base stage b1 pin of triode Q1, the base stage b1 pin of triode Q1 links to each other with an end of resistance R 8, the other end of resistance R 8 links to each other with the 2k pin of double-row needle socket J3, the 2k pin ground connection GND of double-row needle socket J3, emitter e 1 pin of triode Q1 links to each other with the 1k pin of double-row needle socket J3, double-row needle socket J3 links to each other with semiconductor heat booster 11 on the PCR micromechanics chip 9, the break-make of control semiconductor heat booster, the 3h pin of photoelectric isolated chip U4, the 5h pin is unsettled.

The LCD MODULE 6 of the miniature thermal cycling Controlling System of described PCR biochip comprises LCD MODULE, exclusion and potentiometer three parts, the liquid-crystal display that adopts carries driver module, show 2 row, 16 characters of every row, LCD MODULE A2 links to each other with the A1 chip of microprocessor 1, controlled by control line, the control line of the A1 chip of microprocessor 1 links to each other with exclusion A3, the 1m pin ground connection GND of LCD MODULE A2, the 2m pin of LCD MODULE A2 meets power supply VCC3.3, the 3m pin of LCD MODULE A2 links to each other with the 2p pin of potentiometer RP1, the 1p pin of potentiometer RP1 links to each other with power supply VCC3.3, the 3p pin ground connection GND of potentiometer RP1, the 4m pin of LCD MODULE A2, the 5m pin, the 6m pin respectively with the 20f pin of the A1 chip of microprocessor 1, the 21f pin, the 22f pin links to each other; The 7m pin of LCD MODULE A2,8m pin, 9m pin, 10m pin, 11m pin, 12m pin, 13m pin, 14m pin link to each other with 23f pin, 24f pin, 25f pin, 26f pin, 27f pin, 28f pin, 29f pin, the 30f pin of the A1 chip of microprocessor 1 respectively; The 23f pin of the A1 chip of

microprocessor

1,24f pin, 25f pin, 26f pin, 27f pin, 28f pin, 29f pin, 30f pin link to each other with 2n pin, 3n pin, 4n pin, 5n pin, 6n pin, 7n pin, 8n pin, the 9n pin of exclusion A3 respectively, the 15m pin of LCD MODULE A2 meets power supply VCC3.3, the 16m pin ground connection GND of LCD MODULE A2, the 1n pin of exclusion A3 links to each other with power supply VCC3.3.

The key control circuit 7 of the miniature thermal cycling Controlling System of described PCR biochip comprises 3 buttons and 3 resistance, the 1i pin of button S2, the 1j pin of button S3, the 1r pin of button S4 respectively with the 17f pin of the A1 chip of microprocessor 1, the 18f pin, the 19f pin links to each other, the 17f pin of the A1 chip of microprocessor 1, the 18f pin, the 19f pin respectively with an end of resistance R 9, one end of resistance R 10, one end of resistance R 11 links to each other, the other end of resistance R 9, the other end of resistance R 10, another termination power VCC3.3 of resistance R 11, the 2i pin of button S2, the 2j pin of button S3, the 2r pin ground connection GND of button S4.

The JTAG emulation module 8 of the miniature thermal cycling Controlling System of described PCR biochip comprises JTAG chip J1 and button two portions, the 1q pin of the J1 chip of JTAG emulation module 8, the 3q pin, the 5q pin, the 7q pin, the 11q pin respectively with the 54f pin of the A1 chip of microprocessor 1, the 55f pin, the 56f pin, the 57f pin, the 58f pin links to each other, the 9q pin ground connection GND of the J1 chip of JTAG emulation module 8, the 11q pin of the J1 chip of JTAG emulation module 8 links to each other with an end of resistance R 1, the other end of resistance R 1 links to each other with power supply VCC3.3, the 11q pin of the J1 chip of JTAG emulation module 8 links to each other with the 1s pin of button S 1, the 2s pin ground connection GND of button S1, the 2q pin of the J1 chip of JTAG emulation module 8 links to each other with power supply VCC3.3.

The interface section of the modules of the miniature thermal cycling Controlling System of described PCR biochip all adopts socket to draw, the A1 chip of the microprocessor 1 of PCR integrated chip temperature control system with pin also by row pin do not draw.

Principle of work of the present invention is: take microprocessor as core, by the semiconductor heat booster on the PCR micromechanics integrated chip liquid in the reaction microchamber is heated, in the process of system heating, the Pt temperature probe that is integrated in the reaction chamber bottom carries out temperature acquisition and feedback simultaneously, the Pt temperature probe sends temperature signal to bridge diagram by interface, bridge diagram amplifies through the amplifier module after resistance signal is converted to weak voltage signals, voltage signal after the amplification is as the input signal of microprocessor, microprocessor is by carrying after the A/D modular converter transforms, regulate duty by pid algorithm and recently control the break-make of voltage on the semiconductor heat booster, thereby reach liquid heat time and the purpose of regulating Heating temperature in the control reaction chamber.

Advantage of the present invention is: all adopt the low power dissipation electron element, especially microprocessor can enter various low-power consumption modes automatically, adopt pwm pulse width modulated technology, realize the output of executive signal with software, simplified hardware configuration, had that volume is little, low in energy consumption, hardware configuration is simple, cycling time is short, control accuracy is high and power easily advantage.

Description of drawings

Fig. 1 is connection diagram of the present invention.

Fig. 2 is the schematic diagram of PCR micromechanics chip 9 of the present invention.

Fig. 3 is the schematic circuit of microprocessor 1 of the present invention.

Fig. 4 is the schematic circuit of voltage transformation module 2 of the present invention.

Fig. 5 is the schematic circuit of temperature data acquisition of the present invention and amplification module 3.

Fig. 6 is the schematic circuit of output power signal module 4 of the present invention.

Fig. 7 is the schematic circuit of LCD MODULE 6 of the present invention.

Fig. 8 is the schematic circuit of key control circuit 7 of the present invention.

Fig. 9 is the schematic circuit of JTAG emulation module 8 of the present invention.

Embodiment

Below in conjunction with accompanying drawing the present invention is described in detail.

Referring to Fig. 1, Fig. 2, a kind of miniature thermal cycling Controlling System of PCR biochip comprises microprocessor 1, for the treatment of temperature signal and export control signal; Voltage transformation module 2 is used for providing operating voltage to each circuit part; Temperature data acquisition and amplification module 3 amplify for the collecting temperature signal and to signal voltage; Output power signal module 4 is used for providing heat driven power; Terminal block 5 is used for being connected with exterior PC R biochip; LCD MODULE 6 is used for showing thermal circulation parameters; Key control circuit 7 is used for the displaying contents on the selection Liquid Crystal Module; JTAG emulation module 8, for eight major parts of communicating by letter with outside upper computer, the operating voltage input terminus of microprocessor 1 links to each other with the first output terminal of voltage transformation module 2, temperature data acquisition links to each other with the operating voltage input terminus of amplification module 3 and the second output terminal of voltage transformation module 2, the operating voltage input terminus of output power signal module 4 links to each other with the 3rd output terminal of voltage transformation module 2, the operating voltage input terminus of LCD MODULE 6 links to each other with the 4th output terminal of voltage transformation module 2, the operating voltage input terminus of JTAG emulation module 8 links to each other with the 5th output terminal of voltage transformation module 2, temperature data acquisition links to each other with the output terminal of amplification module 3 and the first input end of microprocessor 1, the output terminal of key control circuit 7 links to each other with the second input terminus of microprocessor 1, the first output terminal of microprocessor 1 links to each other with the input terminus of output power signal module 4, the second output terminal of microprocessor 1 links to each other with the input terminus of LCD MODULE 6, the 3rd output terminal of microprocessor 1 links to each other with the input terminus of JTAG emulation module 8, the input terminus of temperature data acquisition and amplification module 3 links to each other with Pt temperature sensor 10 on the PCR micromechanics chip 9 by the first interface of terminal block 5, second interface of the output terminal of output power signal module 4 by terminal block 5 links to each other with semiconductor heat booster 11 on the PCR micromechanics chip 9, terminal block 5 adopts integrated installation method with being connected of PCR micromechanics chip 9, generate the pin of corresponding Pt temperature sensor 10 and semiconductor heat booster 11 at the pinboard of PCR integrated chip 9, adopt the mode of dual-in-line to be connected on the corresponding via hole of system circuit board, guarantee the electrical connection of PCR integrated chip and testing circuit board.

Referring to Fig. 3, the microprocessor 1 of the miniature thermal cycling Controlling System of described PCR biochip comprises cpu chip A1 and peripheral circuit thereof, the 1f pin of cpu chip A1, the 64f pin respectively with an end of capacitor C 1, one end of capacitor C 2 links to each other, the other end of capacitor C 1, the other end ground connection GND of capacitor C 2, the 7f pin of cpu chip A1 and the positive pole of chemical capacitor C5, one end of capacitor C 6 links to each other, the negative pole of chemical capacitor C5, the other end ground connection GND of capacitor C 6, the 8f pin of cpu chip A1 links to each other with the end of quartz oscillator Y1, the other end of quartz oscillator Y1 links to each other with the 9f pin of cpu chip A1, the 11f pin ground connection GND of cpu chip A1, the 52f pin of cpu chip A1, the 53f pin links to each other with the two ends of quartz oscillator Y2 respectively, the two ends of quartz oscillator Y2 more respectively with an end of capacitor C 4, one end of capacitor C 3 links to each other, the other end of capacitor C 4, the other end ground connection GND of capacitor C 3, the 62f pin of cpu chip A1,63f pin ground connection GND, the 1f pin of cpu chip A1, the 64f pin meets power supply VCC3.3.

Referring to Fig. 4, the voltage transformation module 2 of the miniature thermal cycling Controlling System of described PCR biochip comprises voltage transitions chip and two parts of double-row needle socket, adopt 9V carbon powered battery, realize the switch power supply management by software, power supply is effectively distributed to the different assemblies of system, energy consumption when the reduction assembly is idle, extending battery life effectively, the realization system is portable, little and the reduce power consumption of volume, the 9V battery passes through double-row needle socket J4 to system power supply, the output terminal of double-row needle socket J4 links to each other with the input terminus of voltage transitions chip U1, be 5V with voltage transitions, be temperature data acquisition and amplification module 3 power supplies, the output terminal of voltage transitions chip U1 links to each other with the input terminus of double-row needle socket J5, the output terminal of double-row needle socket J5 links to each other with the input terminus of voltage transitions chip U2, be 3.3V with the 5V voltage transitions, be microprocessor 1, output power signal module 4,

LCD MODULE

6 and 8 power supplies of JTAG emulation module, the 1a pin ground connection GND of voltage transitions chip U1, the 2a pin of voltage transitions chip U1 connects an end of capacitor C 9, link to each other with the 3c pin of voltage transitions chip U2 after the positive pole of chemical capacitor C10, the other end of capacitor C 9, the minus earth GND of chemical capacitor C10, the 3a pin of voltage transitions chip U1 connects the positive pole of chemical capacitor C7, one end of capacitor C 8, the positive pole of chemical capacitor C7, one end of capacitor C 8 connects the 2b pin of double-row needle socket J4 again, the negative pole of chemical capacitor C7, the ground connection GND of the other end of capacitor C 8, the 1b pin ground connection GND of double-row needle socket J4, the 1c pin ground connection GND of voltage transitions chip U2, the 2c pin of voltage transitions chip U2 connects an end of capacitor C 13, meet power supply VCC3.3 after the positive pole of chemical capacitor C14, the other end of capacitor C 13, the minus earth GND of chemical capacitor C14, the 3c pin of voltage transitions chip U2 connects the positive pole of chemical capacitor C11, one end of capacitor C 12, the positive pole of chemical capacitor C11, one end of capacitor C 12 connects the 2d pin of double-row needle socket J5 again, the negative pole of chemical capacitor C11, the other end ground connection GND of capacitor C 12, the 1d pin ground connection GND of double-row needle socket J5.

Referring to Fig. 5, the temperature data acquisition of the miniature thermal cycling Controlling System of described PCR biochip and amplification module 3 comprise instrument amplification chip and double-row needle socket two portions, adopt bridge diagram and instrumentation amplifier to realize, bridge diagram is characterised in that the resistance signal with the Pt temperature sensor is converted to the front end weak voltage input signal of amplifier, amplifier can be regulated gain by a resistance and be realized amplifying, double-row needle socket J2 links to each other with Pt temperature probe 10 on the PCR micromechanics chip 9, after bridge diagram, resistance signal is converted to voltage signal, the output terminal of bridge diagram links to each other with the input terminus of instrument amplification chip U3, weak voltage signals is amplified, the output terminal of instrument amplification chip U3 links to each other with the input terminus of microprocessor 1, the 1e pin of instrument amplification chip U3 links to each other with the 2l pin with the 1l pin of potentiometer RP2, the 3l pin of potentiometer RP2 links to each other with the 8e pin of instrument amplification chip U3, the 2e pin of instrument amplification chip U3 links to each other with an end of resistance R 2, the other end of resistance R 2 links to each other with the 2g pin of double-row needle socket J2, the 2e pin of instrument amplification chip U3 links to each other with an end of resistance R 3, the other end ground connection GND of resistance R 3, the 3e pin of instrument amplification chip U3 links to each other with the 1g pin of double-row needle socket J2, the 1g pin of double-row needle socket J2 links to each other with an end of resistance R 4, the other end ground connection GND of resistance R 4, the 4e pin of instrument amplification chip U3,5e pin ground connection GND, the 7e pin of instrument amplification chip U3 meets power supply VCC5.0, and the 6e pin of instrument amplification chip U3 links to each other with the 59f pin of the A1 chip of microprocessor 1.

Referring to Fig. 6, the output power signal module 4 of the miniature thermal cycling Controlling System of described PCR biochip comprises photoelectric isolated chip, triode and double-row needle socket three parts, adopt optocoupler to realize the isolation of photosignal, realize power magnification with the Darlington transistor that driving force is strong, regulate the Duty ratio control well heater of the PWM wave producer that micro-chip carries by pid algorithm, during system works, photoelectric isolated chip U4 is powered by power supply VCC3.3, photoelectric isolated chip U4 links to each other with semiconductor heat booster 11 on the PCR micromechanics chip 9 by triode, microprocessor 1 is controlled the break-make of voltage on the semiconductor heat booster 11 by output PWM ripple, the 1h pin of photoelectric isolated chip U4 links to each other with an end of resistance R 5, the other end of resistance R 5 links to each other with power supply VCC3.3, the 2h pin of photoelectric isolated chip U4 links to each other with the collector electrode c2 pin of triode Q2, the base stage b2 pin of triode Q2 links to each other with an end of resistance R 6, the other end of resistance R 6 links to each other with the 36f pin of the A1 chip of microprocessor 1, the emitter e 2 pin ground connection GND of triode Q2, the 6h pin of photoelectric isolated chip U4 links to each other with the collector electrode c1 pin of triode Q1, the collector electrode c1 pin of triode Q1 links to each other with power supply VCC15.0, the 4h pin of photoelectric isolated chip U4 links to each other with an end of resistance R 7, the other end of resistance R 7 links to each other with the base stage b1 pin of triode Q1, the base stage b1 pin of triode Q1 links to each other with an end of resistance R 8, the other end of resistance R 8 links to each other with the 2k pin of double-row needle socket J3, the 2k pin ground connection GND of double-row needle socket J3, emitter e 1 pin of triode Q1 links to each other with the 1k pin of double-row needle socket J3, double-row needle socket J3 links to each other with semiconductor heat booster 11 on the PCR micromechanics chip 9, the break-make of control semiconductor heat booster, the 3h pin of photoelectric isolated chip U4, the 5h pin is unsettled.

Referring to Fig. 7, the LCD MODULE 6 of the miniature thermal cycling Controlling System of described PCR biochip comprises LCD MODULE, exclusion and potentiometer three parts, be used for showing thermal circulation parameters, LCD MODULE A2 links to each other with the A1 chip of

microprocessor

1,3 control bits and 8 bit data positions by SCM system are controlled, 8 control bits of the A1 chip of microprocessor 1 link to each other with exclusion A3, potentiometer RP1 is used for regulating backlight, the 1m pin ground connection GND of LCD MODULE A2, the 2m pin of LCD MODULE A2 meets power supply VCC3.3, the 3m pin of LCD MODULE A2 links to each other with the 2p pin of potentiometer RP1, the 1p pin of potentiometer RP1 links to each other with power supply VCC3.3, the 3p pin ground connection GND of potentiometer RP1, the 4m pin of LCD MODULE A2, the 5m pin, the 6m pin respectively with the 20f pin of the A1 chip of microprocessor 1, the 21f pin, the 22f pin links to each other; The 7m pin of LCD MODULE A2,8m pin, 9m pin, 10m pin, 11m pin, 12m pin, 13m pin, 14m pin link to each other with 23f pin, 24f pin, 25f pin, 26f pin, 27f pin, 28f pin, 29f pin, the 30f pin of the A1 chip of microprocessor 1 respectively; The 23f pin of the A1 chip of

microprocessor

Referring to Fig. 8, the key control circuit 7 of the miniature thermal cycling Controlling System of described PCR biochip comprises 3 buttons and 3 resistance, output signal control by microprocessor, be used for showing real time temperature on the adjustable liquid crystal display display module, cycle index and cycling time, the 1i pin of button S2, the 1j pin of button S3, the 1r pin of button S4 respectively with the 17f pin of microprocessor chip A1, the 18f pin, the 19f pin links to each other, the 17f pin of microprocessor chip A1, the 18f pin, the 19f pin respectively with an end of resistance R 9, one end of resistance R 10, one end of resistance R 11 links to each other, the other end of resistance R 9, the other end of resistance R 10, another termination power VCC3.3 of resistance R 11, the 2i pin of button S2, the 2j pin of button S3, the 2r pin ground connection GND of button S4.

Referring to Fig. 9, the JTAG emulation module 8 of described PCR integrated chip temperature control system comprises JTAG chip J1 and button two portions, be used as the communication interface of upper computer and microprocessor 1, the 1q pin of the J1 chip of JTAG emulation module 8, the 3q pin, the 5q pin, the 7q pin, the 11q pin respectively with the 54f pin of the A1 chip of microprocessor 1, the 55f pin, the 56f pin, the 57f pin, the 58f pin links to each other, the 9q pin ground connection GND of the J1 chip of JTAG emulation module 8, the 11q pin of the J1 chip of JTAG emulation module 8 links to each other with an end of resistance R 1, the other end of resistance R 1 links to each other with power supply VCC3.3, the 11q pin of the J1 chip of JTAG emulation module 8 links to each other with the 1s pin of button S1, the 2s pin ground connection GND of button S1, the 2q pin of the J1 chip of JTAG emulation module 8 links to each other with power supply VCC3.3.

Principle of work of the present invention is:

In conjunction with the PCR reaction microchamber chip based on the MEMS technology, embedded technology, take the MSP430 microprocessor as core, realize the regulation and control to reaction chamber temperature, thereby realize the purpose of amplification in vitro, the output terminal of voltage transformation module 2 respectively with microprocessor 1, temperature data acquisition and amplification module 3, output power signal module 4, LCD MODULE 6, the operating voltage input terminus of JTAG emulation module 8 links to each other, for providing operating voltage with upper module, in the whole working process of system, Pt temperature sensor 10 on the PCR micromechanics chip 9 links to each other with the input terminus of temperature data acquisition with amplification module 3 by terminal block 5, temperature data acquisition and amplification module 3 are comprised of electric bridge change-over circuit and magnifier two portions, the electric bridge change-over circuit is used for resistance signal is converted to voltage signal, magnifier is used for faint voltage signal is amplified, temperature data acquisition links to each other with the first input end of microprocessor 1 with the output terminal of amplification module 3, the voltage signal that amplifies is input in the microprocessor 1, microprocessor 1 is discernible numerary signal by 12 A/D converters that carry with analog signal conversion, the first output terminal of microprocessor 1 links to each other with the input terminus of output power signal module 4, output power signal module 4 is made of optocoupler and Darlington transistor two portions, optocoupler is used for realizing the photoelectricity isolation, Darlington transistor is used for realizing power drive, adopt PWM (pulse-width modulation) technology and pid algorithm, the output terminal of output power signal module 4 links to each other with semiconductor heat booster 11 on the PCR micromechanics chip 9 by terminal block 5, break-make according to voltage on the instruction control semiconductor heat booster 11 of microprocessor 1, the second output terminal of microprocessor 1 links to each other with the input terminus of LCD MODULE 6, be used for showing thermal circulation parameters, the second input terminus of microprocessor 1 links to each other with the output terminal of key control circuit 7, be used for the displaying contents on the selection Liquid Crystal Module, the 3rd output terminal of microprocessor 1 links to each other with the input terminus of JTAG emulation module 8, be used for communicating by letter with outside upper computer, sequence of control provides good man-machine interface, in real time displays temperature curvilinear motion, thereby monitor whole reaction process, the interface section of modules and microprocessor all do not adopt socket to draw with pin, be conducive to piecemeal and detect and debug, be convenient in this other module of platform enlarging.

In the accompanying drawing: 1 is microprocessor; 2 is voltage transformation module; 3 is temperature data acquisition and amplification module; 4 is the output power signal module; 5 terminal blocks; 6 is LCD MODULE; 7 is key control circuit; 8 is the JTAG emulation module; 9 is the PCR integrated chip; 10 is the Pt temperature probe; 11 is semiconductor heat booster; 1a～3a is 3 pins of voltage transitions chip U1; 1b～2b is 2 pins of double-row needle socket J4; 1c～3c is 3 pins of voltage transitions chip U2; 1d～2d is 2 pins of double-row needle socket J5; 1e～8e is 8 pins of instrument amplification chip U3; 1f～64f is 64 pins of the A1 chip of microprocessor 1; 1g～2g is 2 pins of double-row needle socket J2; 1h～6h is 6 pins of photoelectric isolated chip U4; 1i～2i is 2 pins of button S2; 1j～2j is 2 pins of button S3; 1k～2k is 2 pins of double-row needle socket J3; 1l～3l is 3 pins of potentiometer RP2; 1m～16m is 16 pins of LCD MODULE A2; 1n～9n is 9 pins of exclusion A3; 1p～3p is 3 pins of potentiometer RP1; 1q～14q is 14 pins of JTAG emulation chip J1; 1r～2r is 2 pins of button S4; 1s～2s is 2 pins of button S 1; E1, b1 and c1 are respectively emtting electrode, base stage and the collector electrode of triode Q1; E2, b2 and c2 are respectively emtting electrode, base stage and the collector electrode of triode Q2; C1～C14 is electric capacity; R1～R11 is resistance; Y1 and Y2 are quartz oscillator; VCC3.3 is the 3.3V power supply; VCC5.0 is the 5.0V power supply; VCC15.0 is the 15V power supply; GND is ground connection.

Claims

1. the miniature thermal cycling Controlling System of a PCR biochip, comprise microprocessor (1), it is characterized in that: the operating voltage input terminus of microprocessor (1) links to each other with the first output terminal of voltage transformation module (2), temperature data acquisition links to each other with the operating voltage input terminus of amplification module (3) and the second output terminal of voltage transformation module (2), the operating voltage input terminus of output power signal module (4) links to each other with the 3rd output terminal of voltage transformation module (2), the operating voltage input terminus of LCD MODULE (6) links to each other with the 4th output terminal of voltage transformation module (2), the operating voltage input terminus of JTAG emulation module (8) links to each other with the 5th output terminal of voltage transformation module (2), temperature data acquisition links to each other with the output terminal of amplification module (3) and the first input end of microprocessor (1), the output terminal of key control circuit (7) links to each other with the second input terminus of microprocessor (1), the first output terminal of microprocessor (1) links to each other with the input terminus of output power signal module (4), the second output terminal of microprocessor (1) links to each other with the input terminus of LCD MODULE (6), the 3rd output terminal of microprocessor (1) links to each other with the input terminus of JTAG emulation module (8), the input terminus of temperature data acquisition and amplification module (3) links to each other with Pt temperature sensor (10) on the PCR micromechanics chip (9) by the first interface of terminal block (5), second interface of the output terminal of output power signal module (4) by terminal block (5) links to each other with semiconductor heat booster (11) on the PCR micromechanics chip (9), integrative installation technology is adopted in being connected of terminal block (5) and PCR micromechanics chip (9), and the mode by dual-in-line is connected to the pin of Pt temperature sensor (10) and semiconductor heat booster (11) on the corresponding via hole of system circuit board;

The voltage transformation module (2) of the miniature thermal cycling Controlling System of described PCR biochip comprises voltage transitions chip and two parts of double-row needle socket, the input terminus of double-row needle socket J4 links to each other with battery, the output terminal of double-row needle socket J4 links to each other with the input terminus of voltage transitions chip U1, the output terminal of voltage transitions chip U1 links to each other with the input terminus of double-row needle socket J5, the output terminal of double-row needle socket J5 links to each other with the input terminus of voltage transitions chip U2, the 1a pin ground connection GND of voltage transitions chip U1, the 2a pin of voltage transitions chip U1 connects an end of capacitor C 9, link to each other with the 3c pin of voltage transitions chip U2 after the positive pole of chemical capacitor C10, the other end of capacitor C 9, the minus earth GND of chemical capacitor C10, the 3a pin of voltage transitions chip U1 connects the positive pole of chemical capacitor C7, one end of capacitor C 8, the positive pole of chemical capacitor C7, one end of capacitor C 8 connects the 2b pin of double-row needle socket J4 again, the negative pole of chemical capacitor C7, the other end ground connection GND of capacitor C 8, the 1b pin ground connection GND of double-row needle socket J4, the 1c pin ground connection GND of voltage transitions chip U2, the 2c pin of voltage transitions chip U2 connects an end of capacitor C 13, meet power supply VCC3.3 after the positive pole of chemical capacitor C14, the other end of capacitor C 13, the minus earth GND of chemical capacitor C14, the 3c pin of voltage transitions chip U2 connects the positive pole of chemical capacitor C11, one end of capacitor C 12, the positive pole of chemical capacitor C11, one end of capacitor C 12 connects the 2d pin of double-row needle socket J5 again, the negative pole of chemical capacitor C11, the other end ground connection GND of capacitor C 12, the 1d pin ground connection GND of double-row needle socket J5;

The output power signal module (4) of the miniature thermal cycling Controlling System of described PCR biochip comprises photoelectric isolated chip, triode and double-row needle socket three parts, photoelectric isolated chip U4 links to each other with semiconductor heat booster (11) on the PCR micromechanics chip (9) by triode, microprocessor (1) is controlled the driving of the upper power of semiconductor heat booster (11) by output PWM ripple, the 1h pin of photoelectric isolated chip U4 links to each other with an end of resistance R 5, the other end of resistance R 5 links to each other with power supply VCC3.3, the 2h pin of photoelectric isolated chip U4 links to each other with the collector electrode c2 pin of triode Q2, the base stage b2 pin of triode Q2 links to each other with an end of resistance R 6, the other end of resistance R 6 links to each other with the 36f pin of the A1 chip of microprocessor (1), the emitter e 2 pin ground connection GND of triode Q2, the 6h pin of photoelectric isolated chip U4 links to each other with the collector electrode c1 pin of triode Q1, the collector electrode c1 pin of triode Q1 links to each other with power supply VCC15.0, the 4h pin of photoelectric isolated chip U4 links to each other with an end of resistance R 7, the other end of resistance R 7 links to each other with the base stage b1 pin of triode Q1, the base stage b1 pin of triode Q1 links to each other with an end of resistance R 8, the other end of resistance R 8 links to each other with the 2k pin of double-row needle socket J3, the 2k pin ground connection GND of double-row needle socket J3, emitter e 1 pin of triode Q1 links to each other with the 1k pin of double-row needle socket J3, double-row needle socket J3 links to each other with semiconductor heat booster (11) on the PCR micromechanics chip (9), the break-make of control semiconductor heat booster, the 3h pin of photoelectric isolated chip U4, the 5h pin is unsettled.

2. the miniature thermal cycling Controlling System of a kind of PCR biochip according to claim 1, it is characterized in that: the microprocessor (1) of the miniature thermal cycling Controlling System of described PCR biochip comprises cpu chip A1, the 1f pin of cpu chip A1 is connected with an end of capacitor C 1, the 64f pin of cpu chip A1 links to each other with an end of capacitor C 2, the other end of capacitor C 1, the other end ground connection GND of capacitor C 2, the 7f pin of cpu chip A1 and the positive pole of chemical capacitor C5, one end of capacitor C 6 links to each other, the negative pole of chemical capacitor C5, the other end ground connection GND of capacitor C 6, the 8f pin of cpu chip A1 links to each other with the end of quartz oscillator Y1, the other end of quartz oscillator Y1 links to each other with the 9f pin of cpu chip A1, the 11f pin ground connection GND of cpu chip A1, the 52f pin of cpu chip A1, the 53f pin links to each other with the two ends of quartz oscillator Y2 respectively, the two ends of quartz oscillator Y2 more respectively with an end of capacitor C 4, one end of capacitor C 3 links to each other, the other end of capacitor C 4, the other end ground connection GND of capacitor C 3, the 62f pin of cpu chip A1,63f pin ground connection GND, the 1f pin of cpu chip A1, the 64f pin meets power supply VCC3.3.

3. the miniature thermal cycling Controlling System of a kind of PCR biochip according to claim 1, it is characterized in that: the temperature data acquisition of the miniature thermal cycling Controlling System of described PCR biochip and amplification module (3) comprise the instrument amplification chip, bridge diagram and double-row needle socket three parts, the input terminus of double-row needle socket J2 links to each other with Pt temperature sensor (10) on the PCR micromechanics chip (9), the output terminal of double-row needle socket J2 links to each other with the input terminus of bridge diagram, the output terminal of bridge diagram links to each other with the input terminus of instrument amplification chip U3, the output terminal of instrument amplification chip U3 links to each other with the input terminus of microprocessor (1), the 1e pin of instrument amplification chip U3 links to each other with the 2l pin with the 1l pin of potentiometer RP2, the 3l pin of potentiometer RP2 links to each other with the 8e pin of instrument amplification chip U3, the 2e pin of instrument amplification chip U3 links to each other with an end of resistance R 2, the other end of resistance R 2 links to each other with the 2g pin of double-row needle socket J2, the 2e pin of instrument amplification chip U3 links to each other with an end of resistance R 3, the other end ground connection GND of resistance R 3, the 3e pin of instrument amplification chip U3 links to each other with the 1g pin of double-row needle socket J2, the 1g pin of double-row needle socket J2 links to each other with an end of resistance R 4, the other end ground connection GND of resistance R 4, the 4e pin of instrument amplification chip U3,5e pin ground connection GND, the 7e pin of instrument amplification chip U3 meets power supply VCC5.0, and the 6e pin of instrument amplification chip U3 links to each other with the 59f pin of the A1 chip of microprocessor (1).

4. the miniature thermal cycling Controlling System of a kind of PCR biochip according to claim 1, it is characterized in that: the LCD MODULE (6) of the miniature thermal cycling Controlling System of described PCR biochip comprises LCD MODULE, exclusion and potentiometer three parts, the liquid-crystal display that adopts carries driver module, show 2 row, 16 characters of every row, LCD MODULE A2 links to each other with the A1 chip of microprocessor (1), controlled by control line, the control line of the A1 chip of microprocessor (1) links to each other with exclusion A3, the 1m pin ground connection GND of LCD MODULE A2, the 2m pin of LCD MODULE A2 meets power supply VCC3.3, the 3m pin of LCD MODULE A2 links to each other with the 2p pin of potentiometer RP1, the 1p pin of potentiometer RP1 links to each other with power supply VCC3.3, the 3p pin ground connection GND of potentiometer RP1, the 4m pin of LCD MODULE A2, the 5m pin, the 6m pin respectively with the 20f pin of the A1 chip of microprocessor (1), the 21f pin, the 22f pin links to each other; The 7m pin of LCD MODULE A2,8m pin, 9m pin, 10m pin, 11m pin, 12m pin, 13m pin, 14m pin link to each other with 23f pin, 24f pin, 25f pin, 26f pin, 27f pin, 28f pin, 29f pin, the 30f pin of the A1 chip of microprocessor (1) respectively; The 23f pin of the A1 chip of microprocessor (1), 24f pin, 25f pin, 26f pin, 27f pin, 28f pin, 29f pin, 30f pin link to each other with 2n pin, 3n pin, 4n pin, 5n pin, 6n pin, 7n pin, 8n pin, the 9n pin of exclusion A3 respectively, the 15m pin of LCD MODULE A2 meets power supply VCC3.3, the 16m pin ground connection GND of LCD MODULE A2, the 1n pin of exclusion A3 links to each other with power supply VCC3.3.

5. the miniature thermal cycling Controlling System of a kind of PCR biochip according to claim 1, it is characterized in that: the key control circuit (7) of the miniature thermal cycling Controlling System of described PCR biochip comprises 3 buttons and 3 resistance, the li pin of button S2, the 1j pin of button S3, the 1r pin of button S4 respectively with the 17f pin of the A1 chip of microprocessor (1), the 18f pin, the 19f pin links to each other, the 17f pin of the A1 chip of microprocessor (1), the 18f pin, the 19f pin respectively with an end of resistance R 9, one end of resistance R 10, one end of resistance R 11 links to each other, the other end of resistance R 9, the other end of resistance R 10, another termination power VCC3.3 of resistance R 11, the 2i pin of button S2, the 2j pin of button S3, the 2r pin ground connection GND of button S4.

6. the miniature thermal cycling Controlling System of a kind of PCR biochip according to claim 1, it is characterized in that: the JTAG emulation module (8) of the miniature thermal cycling Controlling System of described PCR biochip comprises JTAG chip J1 and button two portions, the 1q pin of the J1 chip of JTAG emulation module (8), the 3q pin, the 5q pin, the 7q pin, the 11q pin respectively with the 54f pin of the A1 chip of microprocessor (1), the 55f pin, the 56f pin, the 57f pin, the 58f pin links to each other, the 9q pin ground connection GND of the J1 chip of JTAG emulation module (8), the 11q pin of the J1 chip of JTAG emulation module (8) links to each other with an end of resistance R 1, the other end of resistance R 1 links to each other with power supply VCC3.3, the 11q pin of the J1 chip of JTAG emulation module (8) links to each other with the 1s pin of button S1, the 2s pin ground connection GND of button S1, the 2q pin of the J1 chip of JTAG emulation module (8) links to each other with power supply VCC3.3.

7. the miniature thermal cycling Controlling System of a kind of PCR biochip according to claim 1, it is characterized in that: the interface section of the modules of the miniature thermal cycling Controlling System of described PCR biochip all adopts socket to draw, the A1 chip of the microprocessor (1) of PCR integrated chip temperature control system with pin also by row pin do not draw.