CN102146351B - Biocontrol strain 2JW6 for preventing and controlling bacterial wilt of ginger - Google Patents
Biocontrol strain 2JW6 for preventing and controlling bacterial wilt of ginger Download PDFInfo
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- CN102146351B CN102146351B CN201110004718A CN201110004718A CN102146351B CN 102146351 B CN102146351 B CN 102146351B CN 201110004718 A CN201110004718 A CN 201110004718A CN 201110004718 A CN201110004718 A CN 201110004718A CN 102146351 B CN102146351 B CN 102146351B
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- bacterial wilt
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Abstract
The invention relates to a biocontrol strain 2JW6 for preventing and controlling bacterial wilt of ginger in a greenhouse, belonging to the technical field of plant protection. The 2JW6 strain is subjected to 180rpm shake culture in an LB culture solution at 28 DEG C for 12-16 hours, and is then subjected to 6000rpm centrifuging for 10 minutes. The total viable concentration in the finished microbial inoculum product is 1*10<9>-1*10<10>CFU/mL; when being diluted 10-100 times, the product can be used for seed soaking in the ginger planting process or root irrigation in the transplanting process; and the control effect on the bacterial wilt of ginger is 53.36%.
Description
One, technical field
The present invention relates to a kind of biocontrol strain 2JW6 that prevents and treats greenhouse ginger bacterial wilt, belong to the plant protection technology field, be exclusively used in the non-environmental pollution control of ginger bacterial wilt.
Two, background technology
The ginger bacterial wilt is commonly called as " bacterial wilt of ginger ", be by Solanaceae Raul Salmonella (
Ralstonia solanacearum) a kind of bacterial disease of causing.This soil-borne pathogen mainly is distributed in tropical and subtropical zone, and its host range very extensively can infect more than 50 section's 300 various crop.This pathogenic bacteria is mainly propagated by soil, in vascular bundle, breeds in a large number after root infects and causes fascicular obstruction, influences the moisture transportation of host crop, thereby causes the death of wilting.In a single day this disease is very easy to cause crushing blow because geographic range is wide, and host range is wide so infect, up to the present also do not have the effectively preventing means.
Ginger is the important cash crop of China, and bacterial wilt of ginger is at existing report the 1950's, and incidence trend is serious gradually in recent years, and according to investigations, the general diseased plant rate in field is 10%～20%, and the grave illness field is up to 30%～40%, and local plot even total crop failure are lost and received.And, caused serious economy loss because morbidity reduces quality.
For a long time, both at home and abroad to anti-Zhiduo County of bacterial wilt of ginger from cultural control and chemical prevention, control effect is not obvious, and has caused environmental pollution to a certain degree.Therefore, biological control is because the advantage of himself begins to receive increasing attention.In sum, what develop new, more efficient and safe microbial bactericide control bacterial wilt of ginger is the needs that improve the social production total amount, and the while is the needs of agriculture safe Sustainable development especially, symbol social development demand.
Three, summary of the invention
1 technical problem:
To the present situation that the ginger bacterial wilt is not had the biological prevention and control agent of higher preventive effect in the prior art, provide exploitation a kind of single biocontrol microorganisms bacterial strain and microbial inoculum thereof of preventing and treating the ginger bacterial wilt, this microbial inoculum can reach more than 50% the preventive effect of this important soil-borne disease.
Technical scheme
The biocontrol strain 2JW6 of control greenhouse ginger bacterial wilt, for have a liking for Fructus Hordei Germinatus oligotrophy sporangium (
Stenotrophomonas maltophilia), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, address on August 3rd, 2010: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences, culture presevation number is CGMCC NO.4043.
The method that biocontrol strain 2JW6 is used to prepare biocontrol fungicide is:
With above-mentioned biocontrol strain 2JW6 at 28 ℃ of 180 rpm shaking culture of LB nutrient solution 12-16 h, centrifugal 10 min of 6000 rpm then, centrifugal 10 min of 6000 rpm then dilute with aqua sterilisa and to process microbial inoculum, the viable bacteria total concn is 1 * 10 in the microbial inoculum finished product
9-1 * 10
10CFU/mL.
Above-mentioned biocontrol fungicide at the application method aspect the control ginger bacterial wilt does, half a hour is used in the seed soaking before crop is transplanted of 2JW6 bacterial strain preparation, perhaps 9 liters every mu 2JW6 bacterial strain preparations, dilute 100 times after filling root use when transplanting.
3 beneficial effects
Advantage of the present invention and positively effect show: the present invention is the biological prevention and control agent of developing to the ginger bacterial wilt specially.Owing to be biotechnological formulation; Do not have a series of problems that use brought because of chemical pesticide fully, thereby help the nuisanceless production of vegetables, the peasant can or reduce the consumption of other chemical pesticides; This not only can be the peasant and reduces expenses, and helps the outlet of vegetables.Simultaneously, this microbial inoculum increases production function in addition, can be the peasant and increases income.
The greenhouse experiment shows: this bacteria preparation can significantly reduce the generation of bacterial wilt on the ginger.When ginger cultivation, soak seed after this microbial inoculum can dilute 100 times and handle the ginger piece, perhaps when transplanting, irritate root and handle.Preventive effect for the ginger bacterial wilt under greenhouse experiment can reach 53.36% (table 1).
Four embodiments
The preparation of 1 2JW6 biocontrol fungicide
The present invention relates to a kind of biocontrol strain 2JW6 that prevents and treats the ginger bacterial wilt, this bacterial strain is on the healthy tree of the sick field of Laiwu, Shandong ginger, to separate in 2008 to obtain.
With above-mentioned biocontrol strain 2JW6 at 28 ℃ of 180 rpm shaking culture of LB nutrient solution 12-16 h, centrifugal 10 min of 6000 rpm then, centrifugal 10 min of 6000 rpm then dilute with aqua sterilisa and to process microbial inoculum, the viable bacteria total concn is 1 * 10 in the microbial inoculum finished product
9-1 * 10
10CFU/mL.
2 greenhouse basin alms bowl tests detect the preventive effect of 2JW6 biocontrol fungicide to the ginger bacterial wilt
Before the transplanting, with biocontrol microorganisms 2JW6 (10
7CFU/mL) ginger piece 30min is handled in seed soaking; Adopt 20mL 10 during transplanting
7The CFU/mL microbial inoculum is irritated root and is handled, and transplants a week back inoculation 10
7The CFU/mL Ralstonia solanacearum
Ralstonia solanacearum3721 (J G Li
Etal,2008) bacteria suspension 20mL.Handle without biocontrol microorganisms 2JW6 in addition, the processing of direct inoculation Ralstonia solanacearum is as control treatment.Each treatment group 24 strain, three repetitions.25 ~ 28 ℃ of temperature, relative humidity 40% under the illumination 12h/12h condition, is cultivated the sick progression of 4 all " Invest, Then Investigate "s, calculates disease severity and preventive effect.
According to the disease grade scale that Kempe and Sequeria nineteen eighty-three propose, the calculation formula of disease severity and preventive effect is following:
DI 0, do not have morbidity;
DI 1, and≤25% blade is wilted;
The blade of DI 2,25 – 50% is wilted;
The blade of DI 3,50 – 75% is wilted;
The blade wilting (Kempe and Sequeria, 1983) of DI 4,75 – 100%.
Investigation result after transplanting for 4 weeks shows that biocontrol fungicide 2JW6 reaches 53.36% to the preventive effect of ginger bacterial wilt.
Claims (4)
1. biocontrol strain 2JW6 who prevents and treats greenhouse ginger bacterial wilt; Be germ oligotrophy unit cell (Stenotrophomonas maltophilia); Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on August 3rd, 2010, culture presevation number is CGMCC NO.4043.
2. with the biocontrol fungicide of the biocontrol strain 2JW6 of the said control of claim 1 greenhouse ginger bacterial wilt preparation.
3. prepare the method for biocontrol fungicide with the biocontrol strain 2JW6 of the said control of claim 1 greenhouse ginger bacterial wilt, it is characterized in that:
With the biocontrol strain 2JW6 of the said control of claim 1 greenhouse ginger bacterial wilt at 28 ℃ of 180rpm shaking culture of LB nutrient solution 12-16h; The centrifugal 10min of 6000rpm then; The centrifugal 10min of 6000rpm then dilutes with aqua sterilisa and to process microbial inoculum, and the viable bacteria total concn is 1 * 10 in the microbial inoculum finished product
9-1 * 10
10CFU/mL.
4. claim 2 or the 3 said biocontrol fungicides application aspect control ginger bacterial wilt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110004718A CN102146351B (en) | 2011-01-12 | 2011-01-12 | Biocontrol strain 2JW6 for preventing and controlling bacterial wilt of ginger |
Applications Claiming Priority (1)
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|---|---|---|---|
| CN201110004718A CN102146351B (en) | 2011-01-12 | 2011-01-12 | Biocontrol strain 2JW6 for preventing and controlling bacterial wilt of ginger |
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| CN102146351A CN102146351A (en) | 2011-08-10 |
| CN102146351B true CN102146351B (en) | 2012-08-29 |
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| CN105002111B (en) * | 2015-07-14 | 2019-04-30 | 江苏省安丰生物源农药工程中心有限公司 | It is a kind of prevent and treat cucumber downy mildew bacterial strain TDJN1 and its application |
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| CN1932006A (en) * | 2006-10-12 | 2007-03-21 | 上海交通大学 | Prepn process of strain and prepn for controlling blight of vegetable biologically |
| CN101139565B (en) * | 2007-08-24 | 2011-05-11 | 南京农业大学 | Bacterial strain XY21 preventing and curing glasshouse vegetable bacterial wilt |
| CN101177671B (en) * | 2007-12-05 | 2010-06-09 | 南京农业大学 | Biocontrol bacteria strain preventing and curing plant disease |
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