CN102144564B - Method for inducing callus of peony petals - Google Patents
Method for inducing callus of peony petals Download PDFInfo
- Publication number
- CN102144564B CN102144564B CN 201110046787 CN201110046787A CN102144564B CN 102144564 B CN102144564 B CN 102144564B CN 201110046787 CN201110046787 CN 201110046787 CN 201110046787 A CN201110046787 A CN 201110046787A CN 102144564 B CN102144564 B CN 102144564B
- Authority
- CN
- China
- Prior art keywords
- callus
- peony
- medium
- petal
- tree peony
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for inducing callus of peony petals, applying an MS culture medium that contains 6-BA cytokinin, NAA plant growth regulator, 2, 4-D weedicide and the like to induce callus. Specific culture medium scheme and hormone combination are supplied for explants of peony petals. The optimal material drawn period of the callus is described in the invention. The inductivity of the peony callus is more than 80% and a preferable induction effect is achieved; and the inductivity of the petal callus is higher than 50%.
Description
The application divides an application for following application:
Application number: 200910083744.3; Denomination of invention: tree peony callus induction method; The applying date: 2009.5.11.
Technical field
The present invention relates to a kind of callus culture method, relate in particular to a kind of peony petal callus induction method.
Background technology
Tree peony is one of China's ten big famous flowers, also is the national flower of China, and the good reputation of " king in spending " is arranged.The tree peony tissue culture mainly be utilize bud induce plant with utilize explant to induce through the callus approach; And the callus induction of tree peony is an important stage for regeneration plant; Higher callus induction rate lays a good foundation for the later stage differentiation of calli, and kind and the concentration and the condition of culture that how to obtain higher callus induction rate and type of culture medium, hormone have confidential relation.
Callus culture is a kind of modal cultivation form, and except that the shoot apical meristem cultivation with a part of organ culture, other several plant tissue culture forms finally all will experience callus could produce plant.Callus also usually is the source of suspension cultured cells and protoplast in addition.Callus is divided into embryo callus and non-embryonic callus tissue, and embryo callus is meant the callus of somatic embryo, through being divided into the tender plant of children.
In the prior art; The tree peony Study on tissue culture has had long history; Research contents also mainly concentrates on fields such as explant selection, growth regulatory substance proportioning and culture medium prescription, mainly is to utilize stem apex direct development to become plant, and utilizes the explant induction callus; Utilize the research of callus differentiation generation plant less relatively then, and inducing of callus is the precondition that the callus differentiation produces plant.
There is following shortcoming at least in above-mentioned prior art:
There are callus induction difficulty, rooting rate and problems such as difficulty, brownization, vitrifying are transplanted in of low quality, the domestication of taking root.
Summary of the invention
The purpose of this invention is to provide the high peony petal callus induction method of a kind of callus induction rate.
The objective of the invention is to realize through following technical scheme:
Peony petal callus induction method of the present invention comprises and chooses the tree peony explant, adopts the MS medium to carry out inducing of callus, and said tree peony explant is a petal;
Be the period of drawing materials: the monokaryon at flower pesticide is drawn materials mid-term;
Said MS medium comprises: MS+2,4-D1.0mg/L+6-BA2mg/L+NAA0.1mg/L.
Technical scheme by the invention described above provides can be found out; Peony petal callus induction method of the present invention; Owing to adopt the MS medium to carry out inducing of peony petal callus, be furnished with the 6-BA basic element of cell division, NAA plant growth regulator, 2 in the MS medium, the 4-D weed killer herbicide; Be in due course and draw materials, callus induction rate is high.
Embodiment
Peony petal callus induction method of the present invention, its preferable embodiment are to comprise and choose the tree peony explant that employing MS medium carries out inducing of callus, is furnished with following one or more materials in the said MS medium:
The 6-BA basic element of cell division, NAA plant growth regulator, 2, the 4-D weed killer herbicide.
Before carrying out the inducing of callus, can cultivate pre-treatment earlier:
At first, selected tree peony explant is carried out 4 ℃ of low temperature treatment;
Then, utilize 2%NaClO sterilization 30min, 70% alcohol disinfecting 30s, aseptic water washing 3-5 time.
Specific embodiment:
With different explants is example, adopts different culture medium and different culture condition to reach the best effect of inducing.Be that object is set forth respectively below with the different explants:
Inducing of embryo callus:
Cultivation period: annual September, after seed forms;
Cultivate pre-treatment: utilize low temperature (4 ℃) to handle the method that combines with gibberellin treatment, inoculate after utilizing conventional sterilization method (2%NaClO sterilize 30min, 70% alcohol disinfecting 30s, aseptic water washing 3-5 time) to sterilize;
Best medium: MS+NAA0.1mg/L+6-BA1.0mg/L;
Callus induction rate reaches 100%.
Stem section callus induction:
Draw materials period: get the tender stem section of children, at the beginning of 4 months by the end of March spring, the young tender stem section that perhaps forms by seed sprouting;
Cultivate pre-treatment: utilize conventional sterilizing methods (2%NaClO sterilize 30min, 70% alcohol disinfecting 30s, aseptic water washing 3-5 time) to carry out disinfection;
Best medium: MS+NAA0.05mg/L+6-BA2.0mg/L+2,4-D1.0mg/L;
Callus induction rate is more than 80%.
The blade callus induction:
Draw materials period: get the tender blade of children, at the beginning of 4 months by the end of March spring, the young tender blade that perhaps forms by seed sprouting;
Cultivate pre-treatment: utilize conventional sterilizing methods (2%NaClO sterilize 30min, 70% alcohol disinfecting 30s, aseptic water washing 3-5 time) to carry out disinfection;
Optimal medium: MS+6-BA0.1mg/L+2,4-D0.5mg/L;
About callus induction rate 80%.
The petal callus induction:
Draw materials period: in the monokaryon mid-term of flower pesticide;
Cultivate pre-treatment: after handling 8d under 4 ℃ of conditions, carrying out disinfection is seeded to medium to utilize conventional sterilizing methods (2%NaClO sterilize 30min, 70% alcohol disinfecting 30s, aseptic water washing 3-5 time);
Optimal medium: MS+2,4-D1.0mg/L+6-BA2mg/L+NAA0.1mg/L;
About callus induction rate 60%.
Anther callus is induced:
Draw materials period: the monokaryon at flower pesticide is drawn materials mid-term;
Cultivate pre-treatment: after handling 8d under 4 ℃ of conditions, carrying out disinfection is seeded to medium to utilize conventional sterilizing methods (2%NaClO sterilize 30min, 70% alcohol disinfecting 30s, aseptic water washing 3-5 time);
Optimal medium: MS+2,4-D2.0mg/L+6-BA1.5mg/L+NAM.0mg/L, sucrose concentration are 6%;
The inductivity about 50% of callus.
Among the present invention,, clear and definite medium scheme and hormone combinations are arranged all to different explants.And to having carried out comprehensive elaboration the period of drawing materials of the best of callus.The inductivity of tree peony callus has reached and has induced effect preferably all more than 80%; Petal and anther callus are induced also and have been reached more than 50%.
The above; Be merely the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, any technical staff who is familiar with the present technique field is in the technical scope that the present invention discloses; The variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (1)
1. a peony petal callus induction method is characterized in that, comprises choosing the tree peony explant, adopts the MS medium to carry out inducing of callus, and said tree peony explant is a petal;
Be the period of drawing materials: the monokaryon at flower pesticide is drawn materials mid-term;
Said MS medium comprises: MS+2,4-D1.0mg/L+6-BA2mg/L+NAA0.1mg/L;
This method also comprises the cultivation pre-treatment:
At first, selected tree peony explant is carried out 4 ℃ of low temperature treatment;
Then, utilize 2%NaClO sterilization 30min, 70% alcohol disinfecting 30s, aseptic water washing 3-5 time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110046787 CN102144564B (en) | 2009-05-11 | 2009-05-11 | Method for inducing callus of peony petals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110046787 CN102144564B (en) | 2009-05-11 | 2009-05-11 | Method for inducing callus of peony petals |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910083744 Division CN101548647B (en) | 2009-05-11 | 2009-05-11 | Tree peony callus induction method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102144564A CN102144564A (en) | 2011-08-10 |
| CN102144564B true CN102144564B (en) | 2012-09-26 |
Family
ID=44419202
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110046787 Expired - Fee Related CN102144564B (en) | 2009-05-11 | 2009-05-11 | Method for inducing callus of peony petals |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102144564B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109602006B (en) * | 2019-01-09 | 2022-07-01 | 山东贝世康生物科技有限公司 | Culture purification method and application of active substance containing peony stem cells |
-
2009
- 2009-05-11 CN CN 201110046787 patent/CN102144564B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 张玉芳.牡丹愈伤组织诱导的初步研究.《中国优秀硕士学位论文全文数据库（农业科技辑）》.2009,(第4期), * |
| 陈怡平等.紫斑牡丹休眠地下芽在组织培养条件下的发育研究.《西北植物学报》.2003,第23卷(第02期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102144564A (en) | 2011-08-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101548647B (en) | Tree peony callus induction method | |
| CN102172220B (en) | Method for inducing calluses of stems and leaves of tree peony | |
| Park et al. | Improved shoot organogenesis of gloxinia ('Sinningia speciosa') using silver nitrate and putrescine treatment | |
| CN106942051B (en) | A kind of culture medium and propagation method of the tissue-culturing quick-propagation of alum root blade | |
| CN103444523B (en) | Method for quickly introducing embryonic callus through anther to regenerate plant | |
| CN104304030B (en) | A kind of propagation method of oriental hybrid lily | |
| CN102550405A (en) | Breeding method of poplar haploid | |
| CN104782502A (en) | Method for rapidly obtaining regenerated plants of fiber hemps | |
| CN103155862B (en) | Sinocalamus latiflorus flower pesticide inductor embryo the method obtaining regeneration plant | |
| CN102144563B (en) | Method for inducing calluses of peony anther | |
| CN104823852B (en) | A kind of Herba Dendrobii tip of a root tissue culture quick propagation educates method | |
| CN106106178B (en) | A kind of method for tissue culture of candy iris | |
| CN102960243A (en) | Method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis | |
| CN104322370A (en) | Bitter gourd in vitro rapid propagation method | |
| Li et al. | Highly effective organogenesis and somatic embryogenesis of Clivia | |
| CN108464242A (en) | A kind of notable direct method for generation of Lilium tenuifolium somatic embryo for shortening induction time | |
| CN102232359B (en) | In-vitro rapid propagation method of double-petal Jasminum sambac | |
| CN105684898B (en) | Method with plant regeneration occurs for a kind of efficiently induction hybrid santal somatic embryo | |
| CN101836589B (en) | Method of rapid propagation of populus | |
| Zhao et al. | Rescue and in vitro culture of herbaceous peony immature embryos by organogenesis | |
| CN102144564B (en) | Method for inducing callus of peony petals | |
| CN100442971C (en) | Induction of peony embryoid | |
| CN105830926B (en) | A kind of quick breeding method for tissue culture of witchweed | |
| CN108739394A (en) | The quick breeding of Chinese rose of cut flower strain Q6 a kind of, regeneration method | |
| CN103314849A (en) | Wild tomato Solanum sitiens somatic embryo induction method and somatic embryo genetic transformation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120926 Termination date: 20170511 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |