CN102144466A - Method for promoting growth of pods by treating rape buds through 6-benzylaminopurine - Google Patents
Method for promoting growth of pods by treating rape buds through 6-benzylaminopurine Download PDFInfo
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- CN102144466A CN102144466A CN 201010588948 CN201010588948A CN102144466A CN 102144466 A CN102144466 A CN 102144466A CN 201010588948 CN201010588948 CN 201010588948 CN 201010588948 A CN201010588948 A CN 201010588948A CN 102144466 A CN102144466 A CN 102144466A
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Abstract
The invention discloses a method for remarkably promoting the growth of rape pods by applying cytokinin, namely 6-benzylaminopurine, and belongs to a method for promoting the growth of plants by utilizing hormone. The method comprises steps of preparation of treating fluid, selection of treatment period and treatment parts of rape, and execution of an operation method. The method has the advantages of simple operation, lower cost and remarkable effect. When the concentration of the 6-benzylaminopurine is 1ng/L, compared with those of the control, the overall length, the beak length, the pod surface area and the thousand seed weight of mature pods are improved by 36.38, 101.24, 49.5 and 51.52 percent respectively. In addition, the method cannot affect the quality of rape grains. The invention provides technical support for regulating the growth of the rape pods and improving the yield of the rape by utilizing plant hormone.
Description
Technical field
The invention belongs to and utilize hormone to promote the method for plant growing, specifically relate to utilize phytocytomine 6-benzyl aminopurine (6-BA) handling oil cauliflower flower bud to reach and promote the siliqua of oilseed rape growth, thus the method for increase output.
Background technology
Rape is one of most important oil crop of China, its cultivated area and gross output all occupy first place in the world, in the annual edible vegetable oil of producing of China, nearly 50% come from rape (Li Jiana etc. the Chinese oil plant in food and the energy security strategy. Beijing: Chinese agriculture science and technology publishing house, 2004,29-39.).Simultaneously, China also is the importer of grease and oil plant maximum, and the imbalance between supply and demand of edible oil is very outstanding, continue to excavate the yield potential of rape, remains from now on the vital task in one period.
Growing of siliqua of oilseed rape has consequence and effect to grain yield.The fructification of siliqua of oilseed rape and other field crop (paddy rice, wheat, following meter) is compared, tangible difference is arranged, it is not only to exist with important " storehouse " role, formation to yield of rape simultaneously also has crucial " source " effect, in the material that forms grain yield of rapeseed, major part derives from the photosynthesis of angle pericarp.Rice is born forever by the carbon metabolism of measuring the angle fruit and thinks, in the weightening finish material of angle fruit, the photosynthetic product of himself accounts for 70%, and other organ is supplied with the 30%(rice and born forever. and Japanese Crop Science can be kept record, and 1979, (2): 265-270.).Fu Shouzhong etc. studies show that, only the photosynthetic product of angle pericarp to the contribution of output just reach 2/3rds (Fu Shouzhong etc. Jiangsu agricultural science, 1980, (6): 18-21.).The result of study of (1992) such as cold lock tigers is found: the photosynthetic product of angle fruit is the main source of grain yield.(cold lock tiger etc. Acta Agronomica Sinica, 1992,18(4): 250-257.).
Utilization phytohormone Regulation growth and development of plant improves output (being the crop chemistry control technology) has become indispensable important measures in the agricultural production gradually.Compare with the traditional farming technology, the adjusting of plant hormone has many advantages, and it can realize " artificially " regulation and control of plant growth.
At present, people have carried out the research that utilization exogenous plant hormones processing angle really improves crop yield, and various hormones all have facilitation to the growth of siliqua of oilseed rape, wherein, and the facilitation maximum that basic element of cell division diagonal angle really increases.But people all adopt the mode of the direct processing angle fruit of hormone now, and its facilitation effect is not obvious, can make contrast increase 4.43%-5.49% of angle fruit length as the method for using 6-BA to smear the angle fruit.
This method changes the strategy of in the past directly smearing the angle fruit with plant hormone, but bud as the processing object, consider that simultaneously plant epidermis has impermeability to ion, we add Silwet L-77 in treatment fluid.Silwet L-77 is a kind of surfactant that well can act on plant, can impel 6-BA more useful effect in the rape bud.The result shows, our this processing method is particularly when 6-BA concentration is 1ng/L, facilitation effect to the growth of siliqua of oilseed rape is remarkable compared with the control, and handle seed qualities such as total unsaturated fatty acid content of back rape seed and oil content and do not have obvious change, our method will provide the reference of technical method for the purpose that improves yield of rape by the growth that promotes the angle fruit.
Summary of the invention
The purpose of this invention is to provide the method for utilizing basic element of cell division 6-BA significantly to promote the siliqua of oilseed rape growth.The method is simple, efficient.
For achieving the above object, the technical scheme taked of the present invention is:
Utilize basic element of cell division 6-BA significantly to promote the method for siliqua of oilseed rape growth, comprise the steps:
(1) selects the plant of growing way unanimity at the rape initial bloom stage, at first remove main inflorescence and secondary and above side shoot, the one-level of selecting and remain side shoot is removed flower open on the one-level side shoot then, it is pending to stay unopened bud, at last to the selected plant mark of listing;
(2) configuration of treatment fluid: 6-BA is dissolved in containing in the 1/2MS medium that volume ratio is 0.1% Silwet L-77 is mixed with the treatment fluid that concentration is 0.01-1000ng/L; Wherein the 1/2MS medium be in the MS medium each constituent content 1/2;
(3) soaking flower handles: soak and remove new open flower before spending, when soaking flower and handling, with the bud treatment fluid that submerges fully, and shake gently, the processing time is 5 minutes;
(4) every other day soak flower and handle once, total coprocessing 3 times disposes at every turn, and it is pure to preserve moisture, be incubated, protect to put the sheepskin bag, soaks flower and disposes and the sheepskin bag is taken off after a week.
Advantage of the present invention:
1. employing provided by the invention is handled bud and is reached the method that promotes really to grow in the angle, and the effect of really growing in the promotion angle is remarkable, compared with the control, and angle fruit length, angle fruit surface area, the average respectively increase by 36.38%, 49.5%, 51.52% of thousand kernel weight.
2. the 6-BA of utilization handling oil cauliflower flower bud provided by the invention promotes the method for really growing in the angle, and the quality that can not influence the rape seed comprises each constituent content of fatty acid, sulphur glycosides content, oil content, protein content and water content etc.
3. the present invention is simple to operate, cost is lower, and the rape initial bloom stage is the period of carrying out foliar application and disease chemical control, and method provided by the invention can and be executed with blade fertilising and disease chemical control, need not more additional effort.
Description of drawings
Fig. 1 variable concentrations 6-BA HORMONE TREATMENT is to siliqua of oilseed rape total length, long, the wide influence of angle fruit of fruit beak,
Fig. 2 concentration is that really compare at the angle that 1ng/L and 0ng/L6-BA handle,
The influence of Fig. 3 variable concentrations 6-BA diagonal angle fruit surface area,
The influence of every seed number per pod of Fig. 4 variable concentrations 6-BA diagonal angle fruit.
Embodiment
The Silwet L-77 that uses among the present invention, be a kind of surfactant commonly used, (Silwet L-77 is that a kind of chemical mixing reagent is made up of following two kinds of components: the 3-[methoxyl group gathers (ethyoxyl)] propyl group-methyl-two (trimethylsiloxy group) silane (84%) and MEE (16%)) available from GE company; The 6-BA(6-benayl aminopurine that uses) available from Baeyer enlightening company;
The component of the MS medium that uses among the present invention is as follows:
Macroelement (20 *): mother liquid concentration (g/L) working concentration (mg/L)
NH
4NO
3 33 1650
KNO
3 ?38 1900
CaCl
2.2H
2O 8.8 440
CaCl
2 6.64 332
MgSO
4.7H
2O 7.4 370
KH
2PO
4 3.4 170
Trace element (100 *): mother liquid concentration (g/L) working concentration (mg/L)
KI 0.083 0.83
H
3P
2O
3 0.62 6.2
MnSO
4.7H
2O 2.23 22.3
ZnSO
4.7H
2O 0.86 8.6
Na
2MoO
4.2H
2O 0.025 0.25
CuSO
4.5H
2O 0.0025 0.025
CoCl
2.6H
2O 0.0025 0.025
Molysite (100 *): mother liquid concentration (g/L) working concentration (mg/L)
FeSO
4.7H
2O 2.78 27.8
Na
2.EDTA.2H
2O 3.73 37.3
Organic principle (100 *): mother liquid concentration (g/L) working concentration (mg/L)
IV
ACreatine 10 100
IV
BNicotinic acid 0.05 0.5
VB
6 0.05 0.5
VB
1 0.05 0.5
Glycine 0.2 2
Every liter 1/ 2MS medium each component working concentration be 1/ 2 of each component working concentration of every liter of MS.
One, the preparation of treatment fluid
The key component of treatment fluid is as follows: preparation 6-BA final concentration is the mixed solution of 0ng/L, 0.01ng/L, 0.1ng/L, 1ng/L, 10ng/L, 100ng/L or 1000ng/L in the 1/2MS that contains 0.1% silwet L-77.Wherein 0ng/L as a comparison case, all the other are as embodiment 1-6.
Two, test method
Select the plant of growing way unanimity at the initial bloom stage of rape, at first remove main inflorescence and secondary and above side shoot, the one-level of selecting and remain side shoot is removed flower open on the one-level side shoot then, it is pending to stay unopened bud, at last to the selected plant mark of listing.
Carry out treatment fluid on the 3rd day and soak the flower test.Soak and spend preceding inspection whether the flower of new opening is arranged, if having its removal; When the rape bud is soaked the flower processing, the treatment fluid that bud be submerged fully, and shake gently, the processing time is 5 minutes.Every other day soak flower and handle once, total coprocessing 3 times, dispose puts paper bag and is used to preserve moisture at every turn, soaks flower and disposes and paper bag is taken off after a week.
The processing of variable concentrations 6-BA all is provided with 3 repetitions, is that the component of 0ng/L is contrast (CK) with 6-BA concentration.
Three, data analysis: the growth phenotype data analysis of ripe siliqua of oilseed rape
Take a sample after the siliqua of oilseed rape maturation.Collect whole angles fruit that the different disposal inflorescence tied respectively, the total length of measured angular fruit, the fruit beak is long, the angle fruit is wide, the dry weight and the thousand kernel weight of seed number per pod, angle pericarp and seed carry out data analysis.Angle fruit surface area calculates with Clarke formula (Sa=Π dh1+1/3 Π dh2, h1=0.8H, h2=0.2H, H are angle fruit length, and d is an angle fruit mean breadth).
As shown in Figure 1, compared with the control, except that 1000 ng/L, the processing of other different 6-BA concentration, the total length of its angle fruit, the long significance that all shows of fruit beak increase.When wherein 6-BA concentration was 1ng/L, the total length of angle fruit, fruit beak be long, and to change difference the most obvious, respectively contrast increase by 36.38% and 101.24%(Fig. 1 and Fig. 2).
Along with the change of 6-BA concentration, the variation tendency of the total length of angle fruit, fruit beak length is similar.Show as, increase to from 0.01ng/L the process of 1ng/L in 6-BA concentration, angle fruit length change into ascendant trend; When 6-BA concentration continue to improve, length change into downward trend, when 6-BA concentration is 1000 ng/L, compared with the control, there is not significant difference (Fig. 1) in angle length really especially.
Compare with the change of angle fruit length, after the 6-BA processing with variable concentrations, the change degree of angle fruit width is less, and only when 6-BA concentration was 1ng/L, angle fruit width contrasted the change that significance is arranged, and has increased by 9.62% (Fig. 1).
Angle fruit surface area size directly influences the photosynthetic production capacity of angle fruit and the substance accumulation of seed, is an important indicator estimating angle fruit source size.Show (Fig. 3) with Clarke formula result of calculation, the concentration of 6-BA is the surface area effect maximum of the processing diagonal angle fruit of 1ng/L, and comparison is according to increasing by 49.5%; The variation tendency of angle fruit surface area is consistent with angle fruit length variations trend.
Siliqua of oilseed rape be the source organ be again the storehouse organ, the development degree of angle fruit has a significant impact the seed of rape, official's spring cloud etc. studies show that, the length of siliqua of oilseed rape, width and the seed thousand kernel weight relation of being proportionate, so we investigate the influence of variable concentrations 6-BA processing to every seed number per pod of rape and thousand kernel weight.
As shown in Figure 4, different processing does not cause that the significance of the every seed number per pod of angle fruit changes, and this shows that 6-BA handling oil cauliflower flower bud does not cause that the otherness of the every seed number per pod of angle fruit changes.
Thousand kernel weight is the important indicator of investigating rape plant productivity.As shown in table 1, the variation tendency of variable concentrations 6-BA processing diagonal angle fruit thousand kernel weight is similar to the variation tendency of angle fruit surface area.Compared with the control, the thousand kernel weight of different disposal all has significance in various degree to improve, and has the greatest impact when wherein the concentration of 6-BA is for 1ng/L, increases by 51.52%.
In the nutriment that forms grain yield of rapeseed, about 2/3rds derive from the photosynthesis of angle pericarp, and we further investigate the influence of different disposal diagonal angle fruit seed dry weight, pericarp dry weight.After the result showed that 6-BA handles, seed dry weight and pericarp dry weight all had increase in various degree, and effect is the most obvious when 6-BA concentration is 1ng/L, the dry weight of pericarp and seed contrast increase respectively 101.3% and 69.85%(table 2).Show that 6-BA has facilitation for the substance accumulation of angle pericarp and seed.
The influence of the thousand kernel weight of table 1. variable concentrations 6-BA processing angle fruit
The seed of table 2. variable concentrations 6-BA diagonal angle fruit and the influence of pericarp dry weight
Four, utilize indexs such as infrared detection rape grain oil content and protein content
In order to investigate the quality whether 6-BA handles influences the rape seed, we detect indexs such as grain oil content, protein content, sulphur glycosides and water content by near infrared spectroscopy.The result is as shown in table 3, and the sulphur glycosides content of different disposal seed, protein content, water content, oil content do not have significant difference, and generally our method does not exert an influence to the quality of rape seed.(Fang Yan etc. northwest agricultural journal, 2007,16(1): 111
-113. the Yang Cui tinkling of pieces of jade etc. Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology's journal (natural science edition), 2006,34(3): the 61-67. Yang Cui tinkling of pieces of jade etc. and the analytical test journal, 2007,26(1): 29-33.).
Table 3. near infrared spectroscopy detects the influence of variable concentrations 6-BA diagonal angle fruit seed
Five, utilize the gas-chromatography experiment to detect each constituent content of fatty acid of rape seed
In order to investigate the content ratio that 6-BA handles the fatty acid component that whether influences the rape seed, we adopt PE8700 type gas chromatograph (gas chromatography, GC) analyze rape seed fatty acid component, the result is as shown in table 4, and different disposal does not cause that each constituent content otherness of fatty acid changes.(Gao Jianqin etc. Jiangsu agricultural journal, 2008,24(5): 581-585.).
Table 4. gas-chromatography (GC) detects the influence of the content of fatty acid of variable concentrations 6-BA diagonal angle fruit seed
Claims (1)
1. utilize 6-benzyl aminopurine to promote the method for siliqua of oilseed rape growth, comprise the steps:
(1) selects the plant of growing way unanimity at the rape initial bloom stage, at first remove main inflorescence and secondary and above side shoot, the one-level of selecting and remain side shoot is removed flower open on the one-level side shoot then, it is pending to stay unopened bud, at last to the selected plant mark of listing;
(2) configuration of treatment fluid:
6-benzyl aminopurine is dissolved in containing in the 1/2MS medium that volume ratio is 0.1% silwet L-77 is mixed with the treatment fluid that concentration is 0.01-1000ng/L;
(3) soaking flower handles: soak and remove new open flower before spending, when soaking flower and handling, with the bud treatment fluid that submerges fully, and shake gently, the processing time is 5 minutes;
(4) every other day soak flower and handle once, total coprocessing 3 times disposes at every turn, and it is pure to preserve moisture, be incubated, protect to put the sheepskin bag, soaks flower and disposes and the sheepskin bag is taken off after a week.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640670A (en) * | 2012-04-20 | 2012-08-22 | 宁夏大学 | Method for improving fruiting performance of tetraena mongolica maxim |
| CN103250710A (en) * | 2012-02-16 | 2013-08-21 | 陕西美邦农药有限公司 | Plant growth regulator composition containing diethylaminoethyl hexanoate and benzylaminopurine |
| CN105393816A (en) * | 2015-10-30 | 2016-03-16 | 中国科学院东北地理与农业生态研究所 | Method for promoting milking of saline and alkaline land rice through exogenous cytokinin |
| CN106259378A (en) * | 2016-08-05 | 2017-01-04 | 华中农业大学 | A kind of chemical slow release agent, its preparation method and application thereof improving yield of rape and lodging resistance |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1162583A (en) * | 1996-04-17 | 1997-10-22 | 贵阳丰收乐实业公司 | Vegetable plant regulating and nutrimental agent |
| CN1795723A (en) * | 2004-12-22 | 2006-07-05 | 郭勋斌 | Plant growth regulator and production process |
| CN101785466A (en) * | 2010-03-25 | 2010-07-28 | 中国农业科学院油料作物研究所 | Yield increasing element for rape |
-
2010
- 2010-12-15 CN CN 201010588948 patent/CN102144466A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1162583A (en) * | 1996-04-17 | 1997-10-22 | 贵阳丰收乐实业公司 | Vegetable plant regulating and nutrimental agent |
| CN1795723A (en) * | 2004-12-22 | 2006-07-05 | 郭勋斌 | Plant growth regulator and production process |
| CN101785466A (en) * | 2010-03-25 | 2010-07-28 | 中国农业科学院油料作物研究所 | Yield increasing element for rape |
Non-Patent Citations (1)
| Title |
|---|
| 《分子植物育种》 20041231 付绍红等 表面活性剂silwet-77对floral-dip转化甘蓝型油菜效果的影响 第662-663页 1 第02卷, 第05期 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250710A (en) * | 2012-02-16 | 2013-08-21 | 陕西美邦农药有限公司 | Plant growth regulator composition containing diethylaminoethyl hexanoate and benzylaminopurine |
| CN103250710B (en) * | 2012-02-16 | 2016-08-03 | 陕西美邦农药有限公司 | A kind of plant growth regulator composition containing diethyl aminoethyl hexanoate Yu benayl aminopurine |
| CN102640670A (en) * | 2012-04-20 | 2012-08-22 | 宁夏大学 | Method for improving fruiting performance of tetraena mongolica maxim |
| CN105393816A (en) * | 2015-10-30 | 2016-03-16 | 中国科学院东北地理与农业生态研究所 | Method for promoting milking of saline and alkaline land rice through exogenous cytokinin |
| CN106259378A (en) * | 2016-08-05 | 2017-01-04 | 华中农业大学 | A kind of chemical slow release agent, its preparation method and application thereof improving yield of rape and lodging resistance |
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Application publication date: 20110810 |