CN102140133A - Protein ErABF1 related to drought resistance and salt tolerance of plant and encoding gene and application thereof - Google Patents
Protein ErABF1 related to drought resistance and salt tolerance of plant and encoding gene and application thereof Download PDFInfo
- Publication number
- CN102140133A CN102140133A CN 201010618551 CN201010618551A CN102140133A CN 102140133 A CN102140133 A CN 102140133A CN 201010618551 CN201010618551 CN 201010618551 CN 201010618551 A CN201010618551 A CN 201010618551A CN 102140133 A CN102140133 A CN 102140133A
- Authority
- CN
- China
- Prior art keywords
- erabf1
- plant
- gene
- salt
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the field of gene engineering, in particular to a protein ErABF1 related to drought resistance and salt tolerance of a plant and an encoding gene and application thereof. The amino acid sequence of the protein is shown as SEQ ID NO.1, and the gene sequence is shown as SEQ ID NO.2. The protein related to drought resistance and salt tolerance and the encoding gene thereof play very important theoretical and practical roles in improving and enhancing the stress resistance of tobacco, increasing the yield, accelerating the stress-resistant molecular breeding process and effectively saving the water resource.
Description
Technical field
The present invention relates to the genetically engineered field, particularly, the present invention relates to a kind of plant drought, protein related to salt tolerance ErABF1 and encoding gene and application.
Background technology
Abiotic stress such as arid, saline and alkaline is the key constraints that influences crop yield and quality.According to statistics, world's arid, semiarid region and saltings area account for 40.6% of land area.Annual arid causes about 80,000,000,000 kilograms of the underproduction to food crop such as wheats in China, and about more than 500,000,000 mu saltings remains to be opened up wasteland and utilizes.Therefore, how to reduce the loss of grain yield, improve the land utilization ratio in arid, saltings, the resistance that strengthens farm crop has become the difficult problem that present agriculture grain-production needs to be resolved hurrily.
Studies show that drought-enduring, the salt tolerance of plant belongs to the complicated quantitative character of controlled by multiple genes mostly, the resistance of utilizing ordinary method to improve crop is subjected to the restriction of cycle length, excellent germplasm resource shortage.At present, utilize the biotechnology means to be transformed in the crop with drought resisting, salt-resistant related gene many, the resistance of transfer-gen plant is significantly improved.As choline dehydrogenase gene BetA is transformed in the tobacco, salt tolerant, low temperature resistant phenotype (Lilius et al., 1996) have been obtained.Barley HVA1 gene is imported paddy rice, improved the drought tolerance (Casaretto et al., 2003) of transgenic paddy rice.Tobacco Mn-SOD gene is changed in the clover, strengthened the drought tolerance and the survival rate (McKersie et al., 1999) of transgenic alfalfa.Yet plant is subjected to controlled by multiple genes to abiotic stress stress tolerances such as arid, high salt, imports the purpose that the individual feature gene can not play effective enhancing stress resistance of plant.Therefore, setting about from improvement or the transcription factor gene that strengthens a key is more efficiently method and the approach (Liu Qiang etc., 2000) that improves crop anti-adversity.
BZIP (basic leucine zipper) transcription factor protein is extensive, the most conservative proteinoid that distributes in the eukaryote transcription factor, main participation the storage of seeds expression of gene, the generation of light form and the control that organ builds up, also involved in plant is to the reaction (Finkelstein et al., 2002) of various signals in dormin, photoperiod and the growth.At present, according to the textural difference of Arabidopis thaliana bZIP transcription factor, it can be divided into 10 subtribes.Wherein, A subtribe member comprises ABF1, ABF2, ABF3, ABF4 etc., main adjusting ABA, arid, high salt, low temperature, heat, the oxidative stress adverse circumstance responsing reaction of participating in plays crucial effects (Choi et al., 2000 in the resistance that improves plant; Uno et al., 2000; Jakoby et al., 2002; Kang et al., 2002).ABF2 crosses the transgenic arabidopsis of expression, has strengthened arid and oxidative stress patience (Kim et al., 2004a than wild-type; Fujita et al., 2005).The Arabidopis thaliana that changes ABF3, ABF4 gene to the patience of low temperature, heat, oxidative stress than wild-type Arabidopis thaliana obviously strengthen (Kim et al., 2004b).In farm crop, degeneration-resistant relevant bZIP transcription factor gene is separated, as the ABP9 (Wang Lei etc., 2002) of corn, the HvABI5 (Xu et al., 1996) of barley, TRAB1 (Hobo et al., 1999 of paddy rice; Kagaya et al., 2002), OsbZIP23, OsbZIP72 (Xiang et al., 2008; Lu et al., 2009), GmbZIP44, GmbZIP 132 (Liao et al., the 2008a of OsAREB1 (Jin et al., 2010), soybean; Liao et al., 2008b), the SlAREB (Hsieh et al., 2010) of tomato etc., the crossing of these genes expressed and can effectively be strengthened transfer-gen plant to arid, saline and alkaline etc. abiotic stress stress tolerance.
Therefore, it is most important for the resistance of improvement, raising crop to separate, excavate degeneration-resistant relevant bZIP transcription factor gene new, that have significant application value.This research is experiment material with drought resisting, the extremely strong wheat wild relative genus species Xinjiang couchgrass of salt tolerance, separate, obtained degeneration-resistant relevant bZIP transcription factor gene, and it is made up plant expression vector utilize Agrobacterium conversion tobacco (W38),, salt tolerance drought-enduring by transgenic tobacco plant is carried out identified, the preliminary clearly function of this gene, for the initiative of Drought-resistance in Wheat, salt tolerant transgenosis new germ plasm, novel material provides important candidate gene resource, also provide the important theory foundation simultaneously for the breeding of wheat adversity gene engineering.
Summary of the invention
The purpose of this invention is to provide a kind of plant drought, protein related to salt tolerance ErABF1.
A further object of the present invention provides the encoding gene of the above-mentioned plant drought of coding, protein related to salt tolerance ErABF1.
Another object of the present invention provides the recombinant vectors that comprises said gene.
Another object of the present invention provides the transgenic cell line that comprises said gene.
Another object of the present invention provides the application of above-mentioned plant drought, protein related to salt tolerance ErABF1.
Drought resisting provided by the present invention, protein related to salt tolerance ErABF1 derive from drought resisting, Xinjiang couchgrass (Elytrigria repens L.) that salt tolerance is stronger, and aminoacid sequence is shown in SEQ ID NO.1.
Drought resisting, protein related to salt tolerance ErABF1 are made up of 352 amino-acid residues, are bZIP class transcription factors.From the N-terminal 271-331 of sequence 1 amino acids residue is the bZIP structure of guarding, and is nuclear localization sequence from the N-terminal 275-283 of sequence 1 amino acids residue.
SEQ?ID?NO.1：
MDFREEQERI?SSSSSRCADD?AAALTRQRSS?VYSLTFDEFQ?SALDEPGKDF
GSMNMDELLR 60
NIRTAEESQA?IGAGPNATSA?SAAGPDHGGI?QRQGSLTLPR?TLSQKTVDEV
WRDMMFFGGP 120
SASASTAAEA?PPPAQRQQTL?GEVTLEEFLV?RAGVVREDMP?GPPPVSPAPV
AQAPPPQPQM 180
LFPQSNMFAP?MVSPLSLANG?LMTGPFGQGG?GGGGGAATMV?SPSPTGRPVM
SNGFGKVEGL 240
NLSSLSPPPM?PYVFNGGLRG?RKPPAMEKVV?ERRQRRMIKN?RESAARSRQR
KQSYMMELET 300
EVAKLKERNE?ELQRKQAEIL?ERQKNEVFEK?VTRQAGPTSK?RIRLRRTLTG?PW
352
In order to make albumen ErABF1 be convenient to purifying, can connect label as shown in table 1 at proteinic N-terminal of forming by the aminoacid sequence shown in the SEQ ID NO.1 or C-terminal.
The sequence of table 1 label
| Label | Residue | Sequence |
| Poly-Arg | 5-6 (being generally 5) | RRRRR |
| Poly-His | 2-10 (being generally 6) | HHHHHH |
| |
8 | DYKDDDDK |
| Strep-tag?II | 8 | WSHPQFEK |
| c- |
10 | EQKLISEEDL |
SEQ ID NO.1 sequence disclosed according to the present invention, but transcription factor ErABF1 synthetic of the present invention also can be synthesized its encoding gene earlier, carry out the biology expression again and obtain.
ErABF1 encoding gene according to the present invention has nucleotide sequence shown in SEQ ID NO.2.The expression of ErABF1 is subjected to inducing of arid, salt stress.
SEQ?ID?NO.2：
atggacttcc?gggaggagca?ggagcgcatc?agcagcagca?gcagccgctg?cgccgatgac 60
gccgctgcgc?tcacaaggca?gaggtcgtcg?gtctactcgc?tcacgttcga?cgagttccag 120
agcgcgctcg?acgagcccgg?caaggacttc?ggatccatga?acatggacga?gctcctccgc 180
aacatcagga?cggccgagga?gtcgcaggcc?atcggcgccg?ggcccaacgc?cacctcggcc 240
tccgccgcgg?ggccggatca?cggcggcatc?cagcgccagg?gctcgctcac?gctccccagg 300
acgctcagcc?agaagaccgt?cgacgaggtc?tggcgcgaca?tgatgttctt?cggagggccc 360
tccgcctccg?cctccacagc?cgccgaggct?ccaccgccgg?cccagaggca?gcagacgctc 420
ggggaggtca?cgctcgagga?gttcctcgtg?cgcgccggcg?tcgtgcgcga?ggacatgccg 480
gggccgccgc?ccgtgtcgcc?ggcgcccgtg?gcccaggcgc?cgcctccgca?gccgcagatg 540
ctgtttcctc?agagcaacat?gtttgctcct?atggtgagtc?ctctgtcctt?ggccaatggg 600
ttgatgaccg?gacctttcgg?ccagggagga?ggcggtggtg?gtggtgcggc?cactatggta 660
tcgccgtcac?cgacggggag?gccggttatg?tccaacggct?tcggcaaggt?ggaaggcctc 720
aacttgtcct?cgctgtcgcc?gccaccgatg?ccgtatgttt?tcaacggcgg?gctgaggggg 780
aggaagccac?cggccatgga?gaaggtggtc?gagaggaggc?agcggcggat?gatcaagaac 840
cgggagtctg?cggcgaggtc?gcgccagagg?aaacagagtt?atatgatgga?attggagact 900
gaggtggcaa?aacttaaaga?gcggaatgag?gagttgcaga?gaaaacaggc?ggagatacta 960
gagaggcaaa?agaatgaggt?tttcgagaag?gttacccggc?aagctggacc?cacgtcgaag 1020
aggatccgcc?tgcggaggac?gctgacgggc?ccttgg 1056
Contain ErABF1 expression of gene box, recombinant expression vector, transgenic cell line and reorganization bacterium and all belong to protection scope of the present invention.
Available existing plant expression vector construction contains the recombinant expression vector of ErABF1 gene.
Described plant expression vector comprises the double base agrobacterium vector and can be used for the carrier etc. of plant micropellet bombardment.Described plant expression vector also can comprise 3 ' end untranslated zone of foreign gene, promptly comprises the dna fragmentation of polyadenylic acid signal and any other participation mRNA processing or genetic expression.The bootable polyadenylic acid of described polyadenylic acid signal joins 3 ' end of mRNA precursor, and the non-translational region of inducing (Ti) plasmid gene (as kermes synthetic enzyme Nos gene), plant gene 3 ' end to transcribe as the Agrobacterium crown-gall nodule all has similar functions.
When using the gene constructed recombinant plant expression vector of ErABF1, before its transcription initiation Nucleotide, can add any enhancement type promotor or constitutive promoter, as the ubiquitin promoter (Ubiquitin) of cauliflower mosaic virus (CaMV) 35S promoter, corn, they can use separately or be used in combination with other plant promoter; In addition, when using gene constructed plant expression vector of the present invention, also can use enhanser, comprise translational enhancer or transcriptional enhancer, these enhanser zones can be ATG initiator codon or neighboring region initiator codon etc., but must be identical with the reading frame of encoding sequence, to guarantee the correct translation of whole sequence.The source of described translation control signal and initiator codon is widely, can be natural, also can be synthetic.Translation initiation region can be from transcription initiation zone or structure gene.
For the ease of transgenic plant cells or plant being identified and screening, can process used plant expression vector, can produce the enzyme of colour-change or the gene of luminophor (gus gene, luciferase genes etc.) as adding the coding that in plant, to express, have the antibiotic marker thing (gentamicin marker, kantlex marker etc.) of resistance or anti-chemical reagent marker gene (as anti-weedkiller gene) etc.From the security consideration of transgenic plant, can not add any selected marker, directly with adverse circumstance screening transformed plant.
Described recombinant expression vector specifically can be the small segment between the SmaI of pBI121 and XbaI site is substituted by the 35S::ErABF1 that the 1-1056 position deoxynucleotide from 5 ' end of sequence 2 obtains in the sequence table.
Described recombinant expression vector specifically can be the small segment between the SmaI of pBI121 and SpeI site is substituted by the 29A::ErABF1 that the 1-1056 position deoxynucleotide from 5 ' end of sequence 2 obtains in the sequence table.
Another object of the present invention provides a kind of method of cultivating plant with adverse resistance.
The method of cultivation plant with adverse resistance provided by the present invention is that the above-mentioned recombinant expression vector that any contains the ErABF1 gene is imported in the vegetable cell, obtains plant with adverse resistance.
Utilize any carrier that can guide foreign gene in plant, to express, the encoding gene of bZIP transcription factor ErABF1 provided by the present invention is imported vegetable cell, can obtain abiotic stress stress-tolerance power enhanced transgenic cell line and transfer-gen plants such as arid and salt.Carry encoding gene expression vector can Ti-plasmids, Ri plasmid, plant viral vector, directly DNA conversion, microinjection, electricity be led, conventional biological method transformed plant cells or tissue such as agriculture bacillus mediated by using, and the plant transformed tissue cultivating is become plant.By the plant transformed host both can be monocotyledons, also can be dicotyledons, as: tobacco, wheat, capital spend 9, Arabidopis thaliana, paddy rice, corn, cucumber, tomato, willow, turfgrass, lucerne place etc.
Described plant stress tolerance specifically can be the resistance of reverse to abiotic stress, as to or the resistance of reverse of salt stress.
The present invention is an experiment material with drought resisting, Xinjiang couchgrass (Elytrigria repens L.) that salt tolerance is stronger, degeneration-resistant relevant bZIP transcription factor ErABF1 (Elytrigria repens ABA Responsive Element Binding Factor 1) albumen and encoding gene thereof have been obtained, and, significantly improved drought resisting, the salt tolerance of plant with ErABF1 gene importing tobacco.Drought resisting of the present invention, protein related to salt tolerance and encoding gene thereof to improvement, strengthen tobacco resistance, improve output, quicken degeneration-resistant molecular breeding process, and effectively save water resources and have important theoretical and practical significance.
The present invention will be further described below in conjunction with drawings and the specific embodiments.
Description of drawings
Fig. 1 is the cDNA clone of drought resisting, protein related to salt tolerance ErABF1 encoding gene.
Fig. 2 detects for the transgene tobacco pcr analysis, A. transgene tobacco callus differentiation screening, plant regeneration; B. transgene tobacco PCR detects, and 1,2,3,4,5,6,7,8, the 9:35S::ErABF1 transgene tobacco; 10,11,12, the 13:29A::ErABF1 transgene tobacco; The 14:W38 contrast; 15: positive control; 16:DL2000Marker.
A.29A::ErABF1, the strain of 35S::ErABF1 transgene tobacco ties up to the growing state that contains on the 200mM NaCl substratum Fig. 3 is that the transgene tobacco salt tolerance identifies; B. the ErABF1 transgene tobacco expression analysis of on 200mM NaCl salt stress substratum, handling.
Fig. 4 identifies for the transgene tobacco drought tolerance, A.29A::ErABF1 reaches the 35S::ErABF1 transgene tobacco at the growing state that contains on the 2%PEG substratum; B. transgene tobacco is containing expression analysis on the 2%PEG substratum.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.
Experimental technique among the following embodiment if no special instructions, is ordinary method.
Embodiment 1: the cDNA clone of Xinjiang couchgrass drought resisting, the relevant ErABF1 gene of salt tolerant
The Xinjiang couchgrass seedling that grows about 45 days is carried out arid processing 5 hours, extract the total RNA of Xinjiang couchgrass with Trizol.Use 5 ' RACE test kit (5 ' RACE System for Rapid Amplification of cDNA Ends Kit) (GIBCOBRL, CAT.NO.18374-058) and 3 ' RACE test kit (3 ' RACE System for Rapid Amplification of cDNA Ends Kit) (GIBCOBRL CAT.NO.18373-019) obtains the full length sequence 1056bp of ErABF1 gene.
Extract total RNA of Xinjiang couchgrass seedling with Trizol, acquire cDNA with the reverse transcription of superscript II (invitrogen) ThermoScript II.According to ErABF1 gene coding region sequences Design primer P1 and P2.The cDNA that obtains with reverse transcription is a template, carries out pcr amplification with primer P1 and P2.The sequence of primer P1 and P2 is as follows:
P1：5’-CGGATGGACTTCCGGGAGGA-3’，
P2：5’-CCAAGGGCCCGTCAGCGTCC-3’。
The PCR product is carried out 0.8% agarose gel electrophoresis detect, obtain molecular weight and be about band about 1.0-1.1Kb, conform to expected results.Reclaim test kit (TIANGEN) with sepharose and reclaim this fragment.Should reclaim fragment is connected with pGEM-T Easy (Promega), method (Proc Natl Acad Sci with reference to Cohen etc., 69:2110), to connect product transformed into escherichia coli DH5 α competent cell, according to the acillin resistance marker screening positive clone on the pGEM-T Easy carrier, obtain containing the segmental recombinant plasmid of recovery.With T7 on this recombinant plasmid vector and SP6 promoter sequence is that primer carries out nucleotide sequencing to it, the open reading frame (ORF) that sequencing result shows the ErABF1 gene that increases for sequence 2 in the sequence table from 5 ' terminal the 1st to 1056 deoxyribonucleotide, encoding amino acid sequence is the protein of sequence 1 in the sequence table.The recombinant vectors called after pTE-ErABF1 from the ErABF1 gene of 5 ' terminal the 1021st to 1056 deoxyribonucleotide that will contain sequence 2 in the ordered list.
The sequence of ErABF1 gene is compared on Genabnk, and bZIP class transcription factor has higher homology in this gene and the Arabidopis thaliana, and does not find the homologous protein gene in the couchgrass of Xinjiang, proves that the ErABF1 gene is a new gene.
Embodiment 2: cultivate drought resisting, salt tolerant transgenic plant with the ErABF1 gene
1, the structure of recombinant expression vector
1) structure of 35S::ErABF1 recombinant expression vector
The cDNA that obtains with total RNA reverse transcription of Xinjiang couchgrass is a template, carries out pcr amplification with the special primer that contains SmaI and XbaI joint sequence; SmaI and XbaI double digestion PCR product reclaim then, enzyme are cut between the CaMV 35S promoter SmaI and XbaI enzyme cutting site afterwards of product forward insertion carrier pBI121, obtain recombinant vectors 35S::ErABF1.
Primer sequence is as follows:
ErABF1[SmaI]5’-GCGCCCGGGCGGATGGACTTCCGGGAGGA-3’
ErABF1[XbaI]5’-GATTCTAGACCAAGGGCCCGTCAGCGTC-3’
2) structure of 29A::ErABF1 recombinant expression vector
The cDNA that obtains with total RNA reverse transcription of Xinjiang couchgrass is a template, carries out pcr amplification with the special primer that contains SmaI and SpeI joint sequence; SmaI and SpeI double digestion PCR product reclaim then, enzyme are cut between the rd29A promotor SmaI and SpeI restriction enzyme site afterwards of product forward insertion carrier pBI121, obtain recombinant vectors 29A::ErABF1.
Primer sequence is as follows:
ErABF1[SmaI]5’-GCGCCCGGGCGGATGGACTTCCGGGAGGA-3’
ErABF1[SpeI]5’-GATACTAGTCC?AAGGGCCCGTCAGCGTC-3’
The acquisition and the evaluation of embodiment 3 transgene tobaccos
1) acquisition of transgene tobacco
P3 (upstream primer): 5 '-CGGATGGACTTCCGGGAG GA-3 ',
P4 (downstream primer): 5 '-CCAAGGGCCCGTCAGCGTCC-3 '.
29A::ErABF1,35S::ErABF1 transgene tobacco are carried out PCR identify that positive transfer-gen plant can obtain 1Kb left and right sides band through pcr amplification, the result obtains to change 29A::ErABF1 tobacco, each 30 strain of 35S::ErABF1 tobacco.
Simultaneously the pBI121 empty carrier is imported tobacco W38, method is the same, in contrast, obtains commentaries on classics empty carrier tobacco (the transgene tobacco T that screening obtains of 15 strain systems
0Representative is shown).
2) drought-enduring, the salt tolerance evaluation of commentaries on classics ErABF1 gene plant
With T
0In generation, changeed 29A::ErABF1,35S::ErABF1 genetic tobacco plant and T
0Generation change the empty carrier adjoining tree 3 age in week seedling root system move into respectively carry out salt in the substratum that contains 200mM NaCl, 2%PEG, drought stress was handled 35 days, observe phenotype and also take pictures.
The result shows: salt stress was handled after 35 days, and the growth under arid, condition of salt stress of 29A::ErABF1 transfer-gen plant is normal, well developed root system, and leaf color is dark green; The 35S::ErABF1 transfer-gen plant arid, also can normal growth under the condition of salt stress, well developed root system, leaf color is dark green; All empty carrier rotaring gene plant blades all wilting or chlorosis, turn white and cause death.Transgene tobacco is drought-enduring, salt tolerance qualification result proof ErABF1 gene can improve the drought-enduring and salt tolerance of plant.
What Fig. 3 A showed is 29A::ErABF1,35S::ErABF1 transfer-gen plant, 35 days photo of empty carrier transfer-gen plant salt stress processing; What Fig. 3 B showed is the ErABF1 transgene tobacco expression analysis of handling on 200mM NaCl salt stress substratum.
What Fig. 4 A showed is 29A::ErABF1,35S::ErABF1 transfer-gen plant, 35 days photo of empty carrier transfer-gen plant drought stress processing; What Fig. 4 B showed is that transgene tobacco is containing expression analysis on the 2%PEG substratum.
Claims (7)
1. a plant drought, protein related to salt tolerance ErABF1 is characterized in that its aminoacid sequence is shown in SEQ ID NO.1.
2. a plant drought, salt-resistant related gene is characterized in that, the described plant drought of coding claim 1, protein related to salt tolerance ErABF1.
3. plant drought as claimed in claim 2, salt-resistant related gene is characterized in that, its base sequence is shown in SEQ ID NO.2.
4. the recombinant vectors that comprises claim 2 or 3 described plant droughts, salt-resistant related gene.
5. the transgenic cell line that comprises claim 2 or 3 described plant droughts, salt-resistant related gene.
6. the application of the described plant drought of claim 1, protein related to salt tolerance ErABF1.
7. the application of claim 2 or 3 described plant droughts, salt-resistant related gene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106185516A CN102140133B (en) | 2010-12-22 | 2010-12-22 | Protein ErABF1 related to drought resistance and salt tolerance of plant and encoding gene and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106185516A CN102140133B (en) | 2010-12-22 | 2010-12-22 | Protein ErABF1 related to drought resistance and salt tolerance of plant and encoding gene and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102140133A true CN102140133A (en) | 2011-08-03 |
| CN102140133B CN102140133B (en) | 2012-08-22 |
Family
ID=44407945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010106185516A Expired - Fee Related CN102140133B (en) | 2010-12-22 | 2010-12-22 | Protein ErABF1 related to drought resistance and salt tolerance of plant and encoding gene and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102140133B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384935A (en) * | 2016-05-16 | 2017-11-24 | 兰州大学 | A kind of Polygonum viviparum albumen and its coded sequence and application |
| CN110684749A (en) * | 2019-11-13 | 2020-01-14 | 黑龙江八一农垦大学 | Application of corn 3-phosphoglycerol dehydrogenase ZmGPDH4 and coding gene thereof in regulation and control of plant stress tolerance |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1727482A (en) * | 2005-07-12 | 2006-02-01 | 中国科学技术大学 | Salt tolerant, anti-drought gene and a proteins encoded thereof and application from the salt mustard |
| WO2010020143A1 (en) * | 2008-07-29 | 2010-02-25 | 四川贝安迪生物基因工程有限公司 | Genes, proteins and vectors for increaseing tolerance of plants and microbes to abiotic stresses and the use thereof |
-
2010
- 2010-12-22 CN CN2010106185516A patent/CN102140133B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1727482A (en) * | 2005-07-12 | 2006-02-01 | 中国科学技术大学 | Salt tolerant, anti-drought gene and a proteins encoded thereof and application from the salt mustard |
| WO2010020143A1 (en) * | 2008-07-29 | 2010-02-25 | 四川贝安迪生物基因工程有限公司 | Genes, proteins and vectors for increaseing tolerance of plants and microbes to abiotic stresses and the use thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384935A (en) * | 2016-05-16 | 2017-11-24 | 兰州大学 | A kind of Polygonum viviparum albumen and its coded sequence and application |
| CN107384935B (en) * | 2016-05-16 | 2020-10-20 | 兰州大学 | Bigelian plant protein and its coding sequence and application |
| CN110684749A (en) * | 2019-11-13 | 2020-01-14 | 黑龙江八一农垦大学 | Application of corn 3-phosphoglycerol dehydrogenase ZmGPDH4 and coding gene thereof in regulation and control of plant stress tolerance |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102140133B (en) | 2012-08-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101173002B (en) | Plants stress tolerance correlation transcription factor GmWRKY54, encoding gene and application thereof | |
| US9809827B2 (en) | Transgenic maize | |
| CN101906155A (en) | Protein ErNAC7 related to drought and salt resistance of plants and coding gene and application thereof | |
| CN105859860A (en) | Application of disease resistance-related protein to regulation and control of plant disease resistance | |
| CN102718850B (en) | Plant stress tolerance related protein GmP1 and encoding gene and application thereof | |
| CN104120138A (en) | Arabidopsis AtPGK2 gene for enhancing salt tolerance of plants and application of arabidopsis AtPGK2 gene | |
| CN102766610B (en) | Plant drought-resistant relevant protein PvSnRK 2.3 and encoding gene and application thereof | |
| CN110964091B (en) | Wheat drought-resistant heterosis related protein TaNF-YB12 and coding gene and application thereof | |
| CN102399268B (en) | Plant stress tolerance-related transcription factor GmNAC11, coding gene and application thereof | |
| CN103497940B (en) | A kind of plant drought associated protein TaSnRK2.6 and encoding gene thereof and application | |
| CN105296443B (en) | A kind of plant drought, protein related to salt tolerance EeSAPK7 and its encoding gene and application | |
| CN101831436A (en) | Method for breeding adverse-resistant plant | |
| CN105349505B (en) | A kind of plant drought, protein related to salt tolerance AsSnRK and its encoding gene and application | |
| CN101704884B (en) | Plant drought resistance and salt tolerance associated protein EeABF6, coding gene and application thereof | |
| CN102140133B (en) | Protein ErABF1 related to drought resistance and salt tolerance of plant and encoding gene and application thereof | |
| US11492635B2 (en) | Method for improving stress tolerance of plants | |
| CN101260403B (en) | Plant strong salt-resistant gene AtNHXS1 and its coding protein and application | |
| CN104292318A (en) | Draught-resistant related protein TaRBP2 of plant, and encoding gene and application thereof | |
| CN104140462A (en) | Plant salt tolerance related protein GhSnRK2-6, and coding gene and applications thereof | |
| CN101979407B (en) | Plant drought and salt tolerance-related protein TaCRF2 and coding gene thereof and application | |
| CN109402150B (en) | Plant drought-resistant related protein PvSnRK2.6 and coding gene and application thereof | |
| CN105753952A (en) | Plant drought tolerance related protein Tabzip174 as well as coding gene and application thereof | |
| CN103614385B (en) | A gene KT525 is improving the application on plant stress tolerance | |
| CN102911262B (en) | Protein related with plant tolerance and coding gene and applications thereof | |
| CN102146127B (en) | Plant drought resistance and salt tolerance related protein EeNAC9 and encoding gene and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120822 Termination date: 20191222 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |