CN102139012B - Quality control method for kidney tonifying syrup - Google Patents
Quality control method for kidney tonifying syrup Download PDFInfo
- Publication number
- CN102139012B CN102139012B CN 201010104877 CN201010104877A CN102139012B CN 102139012 B CN102139012 B CN 102139012B CN 201010104877 CN201010104877 CN 201010104877 CN 201010104877 A CN201010104877 A CN 201010104877A CN 102139012 B CN102139012 B CN 102139012B
- Authority
- CN
- China
- Prior art keywords
- solution
- medicinal material
- fruit
- thin
- syrup
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The invention relates to a quality control method for kidney tonifying syrup, which comprises the following steps of: identifying ingredients of medlar and shizandra berry by thin-layer chromatography, and measuring the content of an n-butyl alcohol extract. In the quality control method, the quality standard of the kidney tonifying syrup is improved, a thin-layer identification experiment on medlar and shizandra berry is conducted, a detection experiment on other ingredients is conducted, and medlar and shizandra berry are subjected to quality control according to an experiment result; and by the revised quality standard, the quality control of medicines is improved, so that the qualitative and quantitative detection of the medicines are more accurate, and the quality is easy to control.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, relate to the detection method of Chinese medicine, especially a kind of method of quality control of kidney tonifying syrup.
Background technology
The principal ingredient of kidney tonifying syrup is: the fruit of Chinese wolfberry, the fruit of Chinese magnoliavine, raspberry, the seed of Chinese dodder, plantain seed, the preparation method is: the above five tastes, boiling twice, each 1.5 hours, collecting decoction, filter, it is 1.15～1.20 that filtrate is concentrated into relative density, and adding 95% ethanol is 70～75% to containing alcohol, stirs evenly, leave standstill, drawing supernatant concentration to relative density is that 1.20～1.25 (60 ± 5 ℃) add an amount of distilled water, and mixing leaves standstill, filter, filtrate add simple syrup (by answer fluid count 1000ml 20%), Sodium Benzoate (by answering 0.3% of fluid number, and the water dissolving) mixing, the adjustment capacity, measure relative density, leave standstill, draw supernatant, can, and get final product every dress 10ml, 100ml or 120ml.
Function with cure mainly: Tianjing Busuiye medicinal, boosting kidney and strengthening yang, be used in poor health, the deficiency of the kidney impotence, emission, urine is muddy.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art part, a kind of method of quality control of kidney tonifying syrup that can the qualitative detection drug ingedient is provided, this method has that detection means is simple, testing result characteristics more accurately.
The objective of the invention is to be achieved through the following technical solutions:
A kind of method of quality control of kidney tonifying syrup, the step of its method is:
(1) take the fruit of Chinese wolfberry as control medicinal material, thin-layered chromatography is differentiated the fruit of Chinese wolfberry composition in the kidney tonifying syrup;
(2) take schizandrin A as reference substance, take the fruit of Chinese magnoliavine as control medicinal material, thin-layered chromatography is differentiated the Chemical composition of Wuweizi in the kidney tonifying syrup;
(5) mensuration of n-butanol extract content.
And the method that the thin layer of the described fruit of Chinese wolfberry is differentiated is:
1. the preparation of control medicinal material solution: get fruit of Chinese wolfberry control medicinal material, add water, heating is boiled, and lets cool filtration, and filtrate is extracted with the ethyl acetate jolting, and extract is concentrated into about 1ml, in contrast medicinal material solution;
2. the preparation of need testing solution: get the kidney tonifying syrup sample, add normal butyl alcohol and extract the merging extract, be concentrated into about 1ml, as need testing solution;
3. thin-layer chromatography test: draw each 5ul of mentioned solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as bonding agent, take ethyl acetate: chloroform: formic acid=3: 2: 1 is as developping agent, launch, take out, dry, put under the 365nm ultraviolet lamp and inspect, inspect in the test sample chromatogram with the corresponding position of control medicinal material chromatogram on aobvious identical blue-fluorescence spot whether, determine whether contain fruit of Chinese wolfberry composition in the test sample.
And the method that the thin layer of the described fruit of Chinese magnoliavine is differentiated is:
1. the preparation of reference substance solution: it is an amount of to get schizandrin A, adds the chloroform dissolving, makes the solution that every 1ml contains 1mg, in contrast product solution;
2. the preparation of control medicinal material solution: get fruit of Chinese magnoliavine control medicinal material 1g, add methenyl choloride 20ml, added hot reflux 30 minutes, filter, filtrate evaporate to dryness, residue add methenyl choloride 1ml makes dissolving, in contrast medicinal material solution;
3. the preparation of need testing solution: get the kidney tonifying syrup sample, add methenyl choloride 20ml, 1h is extracted in jolting, and extract is concentrated into 1ml;
4. thin-layer chromatography test: draw each 5 μ l of mentioned solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as bonding agent, take sherwood oil: ethyl formate: the upper solution of formic acid=15: 5: 1 is as developping agent, launch, take out, dry, put under the 254nm ultraviolet lamp and inspect, inspect in the test sample chromatogram with reference substance and the corresponding position of control medicinal material chromatogram on aobvious dirty green fluorescence spot whether all, determine whether contain Chemical composition of Wuweizi in the sample.
And the assay method of described n-butanol extract content is:
Precision is measured kidney tonifying syrup sample 20ml, extract with water saturated normal butyl alcohol, merge normal butyl alcohol liquid, put in the evaporating dish that is dried to constant weight, evaporate to dryness, put 105 ℃ of dryings 5 hours, and moved in the exsiccator, cooled off 30 minutes, rapid weighed weight, calculate, and get final product, the kidney tonifying syrup sample contains n-butanol extract must not be less than 2.2% of sample.
Advantage of the present invention and good effect are:
The present invention has carried out raising, perfect to the kidney tonifying syrup quality standard, carried out the thin-layer identification method experiment for the fruit of Chinese wolfberry and the fruit of Chinese magnoliavine, and to other compositions test experience in addition, the fruit of Chinese wolfberry and the fruit of Chinese magnoliavine are brought into the method for quality control according to experiment effect, revised quality standard, improved the quality control of medicine, made the quantitative and qualitative analysis detection of medicine more accurate, quality is more prone to control.
Description of drawings
Fig. 1 is the chromatogram that fruit of Chinese wolfberry thin-layer chromatography of the present invention is differentiated, be followed successively by from left to right: 1,2,3 for three batches of test samples of kidney tonifying syrup (lot number is respectively: 0512131,0510130,0504107), 4, fruit of Chinese wolfberry control medicinal material, 5, fruit of Chinese wolfberry negative sample;
Fig. 2 is the chromatogram that fruit of Chinese magnoliavine thin-layer chromatography of the present invention is differentiated, be followed successively by from left to right: 1,2,3 for three batches of test samples of kidney tonifying syrup (lot number is respectively: 0512131,0510130,0504107), 4, the schizandrin A reference substance, 5, fruit of Chinese magnoliavine control medicinal material, 6, fruit of Chinese magnoliavine negative sample;
Fig. 3 is the chromatogram that seed of Chinese dodder thin-layer chromatography of the present invention is differentiated, is followed successively by from left to right: wherein 1,4,5 are kidney tonifying syrup sample lot number 0512131 (different point sample amount 2 μ l, 5 μ l, 10 μ l), 2, Semen Cuscutae, 3, seed of Chinese dodder negative sample;
Fig. 4 is the chromatogram that seed of Chinese dodder thin-layer chromatography of the present invention is differentiated, is followed successively by from left to right: 1,4,5 three batch samples (lot number 0,512,131 0,510,130 0504107), 2, Semen Cuscutae, 3, seed of Chinese dodder negative sample;
Fig. 5 is the chromatogram that seed of Chinese dodder thin-layer chromatography of the present invention is differentiated, is followed successively by from left to right: 1,2,3 three batch samples (lot number 0,504,107 0,510,130 0512131), 4, Semen Cuscutae, 5, seed of Chinese dodder negative sample.
Fig. 6 is the chromatogram that fruit of Chinese wolfberry thin-layer chromatography of the present invention is differentiated, is followed successively by from left to right: 1, betaine reference substance, 2,3,4, be respectively kidney tonifying syrup sample (lot number 0,504,107 0,510,130 0512131), 5, fruit of Chinese wolfberry negative sample.
Embodiment
Below in conjunction with embodiment, the present invention is further described, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
A kind of method of quality control of kidney tonifying syrup, the step of its method is:
(1) thin layer of the fruit of Chinese wolfberry is differentiated
1. the preparation of control medicinal material solution: get fruit of Chinese wolfberry control medicinal material 2g, add water 35ml, heating was boiled 15 minutes, let cool filtration, and filtrate was extracted 20 minutes with ethyl acetate 15ml jolting, and extract is concentrated into about 1ml, in contrast medicinal material solution.
2. the preparation of need testing solution: get kidney tonifying syrup sample 20ml, add normal butyl alcohol and extract 3 times, each 20ml merges extract, is concentrated into about 1ml, as need testing solution.
3. the preparation of negative sample solution: make fruit of Chinese wolfberry negative sample by former medicine preparation technology, prepare negative sample solution according to the need testing solution preparation method.
4. thin-layer chromatography test: according to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B) experiment, draw each 5ul of mentioned solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as bonding agent, take ethyl acetate: chloroform: formic acid=3: 2: 1 is as developping agent, launch, take out, dry, put under the 365nm ultraviolet lamp and inspect, in the test sample chromatogram, with the aobvious identical blue-fluorescence spot in the corresponding position of control medicinal material chromatogram, and negative sample the results are shown in photo 1 without this spot.Test findings: this method specificity is strong, favorable reproducibility, and the spot colour developing is clear, easily judges, so with in this method inclusive criteria.
(2) thin layer of the fruit of Chinese magnoliavine is differentiated
1. the preparation of reference substance solution: it is an amount of to get schizandrin A, adds the chloroform dissolving, makes the solution that every 1ml contains 1mg, in contrast product solution.
2. the preparation of control medicinal material solution: get fruit of Chinese magnoliavine control medicinal material 1g, add methenyl choloride 20ml, added hot reflux 30 minutes, filter, filtrate evaporate to dryness, residue add methenyl choloride 1ml makes dissolving, in contrast medicinal material solution.
3. the preparation of need testing solution: get kidney tonifying syrup sample 20ml, add methenyl choloride 20ml, 1h is extracted in jolting, and extract is concentrated into 1ml.
4. the preparation of negative sample solution: make fruit of Chinese magnoliavine negative sample by former medicine preparation technology, prepare negative sample solution according to the need testing solution preparation method.
(5) thin-layer chromatography test: according to thin-layered chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), draw each 5 μ l of mentioned solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as bonding agent, take sherwood oil (30-60 ℃): ethyl formate: the upper solution of formic acid=15: 5: 1 is as developping agent, launch, take out, dry, put under the 254nm ultraviolet lamp and inspect.In the test sample chromatogram, with reference substance and the corresponding position of control medicinal material chromatogram on equal aobvious dirty green fluorescence spots, and negative sample the results are shown in photo 2 without this spot.
Take fruit of Chinese magnoliavine control medicinal material and schizandrin A as contrast, all can detect the fruit of Chinese magnoliavine in the kidney tonifying syrup.In practical operation, test take schizandrin A as contrast, easier, so the qualitative discriminating to the fruit of Chinese magnoliavine is adopted take schizandrin A as contrast in quality standard.Through test, this method favorable reproducibility, specificity are strong, and the spot colour developing is clear, easily judge, so with in this method inclusive criteria.
(3) thin layer of the seed of Chinese dodder is differentiated
1. carry out thin layer test by method in the WS3-B-3973-98 standard, in the test sample chromatogram of the different point sample amounts of result with the corresponding position of Semen Cuscutae chromatogram on all without obvious spot, the results are shown in photo 3.
2. change sample extraction solvent in the WS3-B-3973-98 standard into normal butyl alcohol, according to the check of former method, as a result in the test sample chromatogram of different batches with the corresponding position of Semen Cuscutae chromatogram on all without obvious spot, the results are shown in photo 4.
3. change the developping agent in the WS3-B-3973-98 standard into cyclohexane: ethyl acetate: formic acid=5: 8: 3, according to former method check, as a result in the test sample chromatogram of different batches with the corresponding position of Semen Cuscutae chromatogram on all without obvious spot, the results are shown in photo 5.
4. experiment conclusion: according to above test, can't detect corresponding spot in the sample according to seed of Chinese dodder thin-layer identification method in the WS3-B-3973-98 standard, extract solvent and adjust developping agent through change and do not detect yet corresponding spot, and in the version pharmacopeia in 2005 without seed of Chinese dodder control medicinal material.Therefore, this thin layer of suggestion cancellation is differentiated.
(4) fruit of Chinese wolfberry (betaine) assay
The fruit of Chinese wolfberry mainly contains the compositions such as polysaccharide and betaine, and because this preparation is syrup, measuring the polysaccharide composition has interference, so only to the betaine composition is measured.According to Chinese Pharmacopoeia version in 2005 and bibliographical information, its method for quantitatively determining is thin layer chromatography scanning at present, carries out following test.
1. thin-layer chromatography condition: developping agent: acetone-absolute ethyl alcohol: hydrochloric acid=10: 6: 1; Developer: bismuth potassium iodide test solution; Thin layer plate: the silica G plate, with before putting into 105 ℃ of activation of baking oven 1h;
2. reference substance solution preparation: precision takes by weighing at 105 ℃ of betaine HCL reference substances that are dried to constant weight an amount of, adds the reference substance solution that ethanol is made 3.982mg/ml.
3. need testing solution preparation: the accurate kidney tonifying syrup sample 20ml that draws, add 95% ethanol to syrup and contain the alcohol amount and reach 80%, leave standstill, filter, filtrate is concentrated into about 1ml, changes in the 5ml measuring bottle, adds ethanol and is diluted to scale.
4. test: accurate each the 5 μ l of mentioned solution that draw, put respectively on same silica gel g thin-layer plate, launch about 10cm, take out, dry, the spray developer the results are shown in photo 6.
5. testing result: the betaine reference substance solution is at the aobvious Chinese red spot of thin layer plate, and the aobvious negative reaction of sample, illustrate in the test sample can not to carry out so the betaine thin layer scanning is tested without the betaine composition.
(5) n-butanol extract: precision is measured kidney tonifying syrup sample 20ml, extracts 5 (30ml, 20ml with water saturated normal butyl alcohol, 20ml, 20ml, 20ml), merge normal butyl alcohol liquid, put in the evaporating dish that is dried to constant weight evaporate to dryness, put 105 ℃ of dryings 5 hours, move in the exsiccator, cooled off rapid weighed weight 30 minutes, calculate, and get final product.
For investigating the extracted amount of each time normal butyl alcohol, in order to determine extraction time, 1,2,3 n-butanol extracting liquid is merged, No. the 4th, 5,6 time extract is placed separately, puts respectively in the evaporating dish that is dried to constant weight, and operation the results are shown in following table as stated above.
Table 1 n-butanol extract test findings
Found out by above-mentioned test findings, the 6th extract percentage composition seldom, only 0.497%, can ignore.So this test only needs to extract to get final product for 5 times.
According to said method the kidney tonifying syrup reserved sample observing sample of 9 batches of different time productions is done n-butanol extract and measure, the result is as follows.
Table 2 n-butanol extract test findings (n=2)
According to above-mentioned 10 batch sample measurement results, determine the n-butanol extract content limit, that is:
10 batch sample average contents, 3.21 * 70%=2.2%.
This product contains n-butanol extract must not be less than 2.2%.
Claims (1)
1. the detection method of a kidney tonifying syrup, it is characterized in that: the step of its method is:
(1) take the fruit of Chinese wolfberry as control medicinal material, thin-layered chromatography is differentiated the fruit of Chinese wolfberry composition in the kidney tonifying syrup;
(2) take schizandrin A as reference substance, take the fruit of Chinese magnoliavine as control medicinal material, thin-layered chromatography is differentiated the Chemical composition of Wuweizi in the kidney tonifying syrup;
(3) mensuration of n-butanol extract content;
The method that the thin layer of the described fruit of Chinese wolfberry is differentiated is:
1. the preparation of control medicinal material solution: get fruit of Chinese wolfberry control medicinal material, add water, heating is boiled, and lets cool filtration, and filtrate is extracted with the ethyl acetate jolting, and extract is concentrated into about 1ml, in contrast medicinal material solution;
2. the preparation of need testing solution: get the kidney tonifying syrup sample, add normal butyl alcohol and extract the merging extract, be concentrated into about 1ml, as need testing solution;
3. thin-layer chromatography test: draw each 5ul of mentioned solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as bonding agent, take ethyl acetate: chloroform: formic acid=3: 2: 1 is as developping agent, launch, take out, dry, put under the 365nm ultraviolet lamp and inspect, inspect in the test sample chromatogram with the corresponding position of control medicinal material chromatogram on aobvious identical blue-fluorescence spot whether, determine whether contain fruit of Chinese wolfberry composition in the test sample;
The method that the thin layer of the described fruit of Chinese magnoliavine is differentiated is:
1. the preparation of reference substance solution: it is an amount of to get schizandrin A, adds the chloroform dissolving, makes the solution that every 1ml contains 1mg, in contrast product solution;
2. the preparation of control medicinal material solution: get fruit of Chinese magnoliavine control medicinal material 1g, add methenyl choloride 20ml, added hot reflux 30 minutes, filter, filtrate evaporate to dryness, residue add methenyl choloride 1ml makes dissolving, in contrast medicinal material solution;
3. the preparation of need testing solution: get the kidney tonifying syrup sample, add methenyl choloride 20ml, 1h is extracted in jolting, and extract is concentrated into 1ml;
4. thin-layer chromatography test: draw each 5 μ l of mentioned solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as bonding agent, take sherwood oil: ethyl formate: the upper solution of formic acid=15: 5: 1 is as developping agent, launch, take out, dry, put under the 254nm ultraviolet lamp and inspect, inspect in the test sample chromatogram with reference substance and the corresponding position of control medicinal material chromatogram on aobvious dirty green fluorescence spot whether all, determine whether contain Chemical composition of Wuweizi in the sample;
The assay method of described n-butanol extract content is:
Precision is measured kidney tonifying syrup sample 20ml, extract with water saturated normal butyl alcohol, merge normal butyl alcohol liquid, put in the evaporating dish that is dried to constant weight, evaporate to dryness, put 105 ℃ of dryings 5 hours, and moved in the exsiccator, cooled off 30 minutes, rapid weighed weight, calculate, and get final product, this product contains n-butanol extract must not be less than 2.2% of sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010104877 CN102139012B (en) | 2010-02-03 | 2010-02-03 | Quality control method for kidney tonifying syrup |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010104877 CN102139012B (en) | 2010-02-03 | 2010-02-03 | Quality control method for kidney tonifying syrup |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102139012A CN102139012A (en) | 2011-08-03 |
| CN102139012B true CN102139012B (en) | 2013-01-09 |
Family
ID=44406954
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010104877 Active CN102139012B (en) | 2010-02-03 | 2010-02-03 | Quality control method for kidney tonifying syrup |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102139012B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113514596A (en) * | 2021-04-28 | 2021-10-19 | 广州科曼生物科技有限公司 | Small and medium polarity control extract of wolfberry fruit and preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456298A (en) * | 2003-04-10 | 2003-11-19 | 毛友昌 | Sugarless kidney composite medicines and preparation thereof |
| CN1569049A (en) * | 2003-07-13 | 2005-01-26 | 毛友昌 | Novel preparing method of |
| CN1814127A (en) * | 2005-11-25 | 2006-08-09 | 毛晓敏 | Shenbao preparation, its preparing method and quality control method |
| CN1840059A (en) * | 2006-01-10 | 2006-10-04 | 毛晓敏 | Pill preparation of 'Shen Bao', its preparation method and quality control method |
| CN1876089A (en) * | 2005-06-09 | 2006-12-13 | 张友生 | A pharmaceutical composition for treating kidney-qi deficiency syndrome and preparation method thereof |
| CN101417038A (en) * | 2007-10-23 | 2009-04-29 | 北京亚东生物制药有限公司 | Traditional Chinese medicine composition for treating male infertility, preparation method and quality control method thereof |
-
2010
- 2010-02-03 CN CN 201010104877 patent/CN102139012B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456298A (en) * | 2003-04-10 | 2003-11-19 | 毛友昌 | Sugarless kidney composite medicines and preparation thereof |
| CN1569049A (en) * | 2003-07-13 | 2005-01-26 | 毛友昌 | Novel preparing method of |
| CN1876089A (en) * | 2005-06-09 | 2006-12-13 | 张友生 | A pharmaceutical composition for treating kidney-qi deficiency syndrome and preparation method thereof |
| CN1814127A (en) * | 2005-11-25 | 2006-08-09 | 毛晓敏 | Shenbao preparation, its preparing method and quality control method |
| CN1840059A (en) * | 2006-01-10 | 2006-10-04 | 毛晓敏 | Pill preparation of 'Shen Bao', its preparation method and quality control method |
| CN101417038A (en) * | 2007-10-23 | 2009-04-29 | 北京亚东生物制药有限公司 | Traditional Chinese medicine composition for treating male infertility, preparation method and quality control method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 中华人民共和国卫生部药典委员会编.益肾糖浆.《卫生部颁药品标准（中药成方制剂第十五册）》.1998, * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102139012A (en) | 2011-08-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103869041B (en) | A kind of detection method of cough-relieving combination of oral medication | |
| CN101618119A (en) | Quality standard of Sinopanax formosanus pills | |
| CN101653483B (en) | Method for controlling quality of fukean tablet | |
| CN101703611A (en) | Quality detection method of Chinese angelica oral liquid for benefiting blood | |
| CN100437112C (en) | Method for inspecting Chinese medicinal preparation quality in treatment of old man eyes dieases | |
| CN102139039B (en) | Quality control method of coptis tablet for clearing away stomach heat | |
| CN101982189A (en) | Method for detecting salvia heart-soothing capsules | |
| CN101204434A (en) | Quality standard for thrombus dispelling pill and test method thereof | |
| CN101797277B (en) | Method for detecting Jingan capsule | |
| CN102139012B (en) | Quality control method for kidney tonifying syrup | |
| CN101703610A (en) | Quality detection method of Qingnao antihypertensive tablet | |
| CN101979027B (en) | Method for detecting Jinze coronary disease capsules | |
| CN109459515B (en) | Herba epimedii control extract (arrow leaf) and application thereof | |
| CN102967684A (en) | Yanning capsule quality standard detection method | |
| CN111189944A (en) | Method for simultaneously identifying radix sophorae flavescentis and common andrographis herb in three-flavor bistort rhizome oral liquid | |
| CN100372563C (en) | Compound preparation for treating bronchitis, its preparation method and quality control method | |
| CN102707006A (en) | Quality detection method of cudrania tricuspidata formula granules | |
| CN112051352B (en) | New method for controlling quality of Fukean tablets | |
| CN101011450B (en) | Quality control method for lucid ganoderma gynostemma pentaphylla oral liquid | |
| HAN et al. | Simultaneous determination of cephaeline and emetine in ipecac and its preparations using RP-HPLC | |
| CN110018269B (en) | Thin-layer identification method for salt flatstem milkvetch seed formula granules | |
| CN113495110A (en) | Method for simultaneously measuring 4 effective components in dandelion bluish green blue particles | |
| CN112946129A (en) | Quality detection method of antidiarrheal syrup | |
| CN101703656A (en) | Method for detecting quality of capsules for regulating menstruation and activating blood | |
| CN101940655B (en) | Detecting method of rehmannia-leaf total-glycoside capsule |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: No.21, 10th Street, Binhai New Area Economic and Technological Development Zone, Tianjin 300457 Patentee after: Jinyao Darentang Group Co.,Ltd. Longshunrong Pharmaceutical Factory Address before: 300457 No. tenth, 21 Avenue, Tianjin economic and Technological Development Zone Patentee before: Longshunrong Pharmaceutical Factory, Tianjin Zhongxin Pharmaceutical Group Co.,Ltd. |