CN102134580A - Method for improving hydrogen production by photosynthetic bacteria using bubbling process - Google Patents
Method for improving hydrogen production by photosynthetic bacteria using bubbling process Download PDFInfo
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- CN102134580A CN102134580A CN 201110005740 CN201110005740A CN102134580A CN 102134580 A CN102134580 A CN 102134580A CN 201110005740 CN201110005740 CN 201110005740 CN 201110005740 A CN201110005740 A CN 201110005740A CN 102134580 A CN102134580 A CN 102134580A
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- photosynthetic bacteria
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Abstract
The invention discloses a method for improving hydrogen production by photosynthetic bacteria using a bubbling process, which comprises the following steps: inoculating a bacterial suspension of photosynthetic bacteria cultured to a logarithmic growth phase into a sterilized medium, reloading the medium into a bubbling photo-biochemical reactor, and producing hydrogen by culturing in a sequential batch mode; during hydrogen production, bubbling once every 3-12 hours with a gas flow of 0.2 L/min, each for 5 minutes. The invention enhances the hydrogen-producing performance of photosynthetic bacteria by blowing Ar into the bubbling photo-biochemical reactor, so on one hand, the bacterial liquid is stirred and the homogeneous mixing requirement for the substrate and the bacteria is satisfied, and on the other hand, the product gas dissolved in the liquid medium is blown out by the mass transfer effect between the gas and the liquid, which reduces the product inhibition effect during the hydrogen production process and enhances the hydrogen-producing performance of photosynthetic bacteria.
Description
Technical field
The present invention relates to a kind of method that can improve the hydrogen production with photosynthetic bacteria performance, belong to the bioenergy field, relate in particular to a kind of method of utilizing bubbling to improve hydrogen production with photosynthetic bacteria.
Background technology
The energy is the important substance basis of national economy and social development, also is one of national strategy safety control basis.Human society is more and more to the demand of energy quantity, and the requirement of quality is also more and more higher.Along with a large amount of exploitations, use and the long-term consumption serious environmental pollution problem that brings of fossil energy, be the one of the main reasons that causes atmosphere and other broad variety environmental pollutions and ecological damage simultaneously.Reduce the application of traditional fossil oil, protect global environment and ecology that we depend on for existence in the time of exploitation and use Energy resources, become global key subjects.Hydrogen Energy is because efficient, renewable and free of contamination characteristic is considered to the most potential future source of energy.Produce hydrogen methods with tradition and compare, biological hydrogen production method is significant to the renewable energy source and the minimizing environmental pollution of development clean and effective, meets the strategy of sustainable development.
Produce the hydrogen microorganism with other and compare hydrogen production with photosynthetic bacteria theoretical yield height; Spectral response range wide (400-900 nm); There is not the oxygen restraining effect; Can consume the organic acid in the waste water, therefore can be coupled producing hydrogen and light-use, organic matter removal, be a kind of very promising product hydrogen mode.
In the prior art, hydrogen production with photosynthetic bacteria is the same with the method for other biological hydrogen manufacturing, and subject matter is that hydrogen-producing speed is lower, and there is bigger distance in the reactor design complexity from suitability for industrialized production and practical application.
Summary of the invention
At the prior art above shortcomings, the invention provides a kind of hydrogen-producing speed of bubbling raising photosynthetic bacterium and product hydrogen methods of hydrogen output of utilizing.
The method of utilizing bubbling to improve hydrogen production with photosynthetic bacteria provided by the invention, it is characterized in that: the photosynthetic bacterium bacterial suspension inoculation that will be cultured to logarithmic phase is in culture medium after sterilization, reinstall in the bubbling style photoproduction reactor, adopt the preface batch culture mode for hydrogen production; In cultivating product hydrogen process, carry out bubbling one time every 3～12 h, gas flow is 0.2 L/min, each 5 minutes.
Beneficial effect of the present invention: the present invention adopts bubbling style photoproduction reactor to carry out hydrogen production with photosynthetic bacteria, by regularly in bubbling style photoproduction reactor, advertising Ar gas, played stirring bacterium liquid, the reinforcement substratum mixes with thalline, simultaneously dissolved product gas stripping from liquid phase in the substratum is come out, reduce the product inhibition in the hydrogen production with photosynthetic bacteria process, the metabolism growth that so more helps photosynthetic bacterium, increase the cell concentration of photosynthetic bacterium, improved the product hydrogen performance of photosynthetic bacterium.
Description of drawings
Fig. 1 (a) be at interval under the 3h bubbling condition with the graphic representation of bubbling condition hypothallus concentration changes with time not;
Fig. 1 (b) be at interval under the 3h bubbling condition with bubbling condition not under medium pH value time history plot;
Fig. 2 (a) be at interval under the 6h bubbling condition with bubbling condition not under medium pH value time history plot;
Fig. 2 (b) be at interval under the 6h bubbling condition with bubbling condition not under medium pH value time history plot;
Fig. 3 (a) accumulates aerogenesis volume time history plot under the bubbling condition under the 12h bubbling condition of interval with;
Fig. 3 (b) be at interval under the 12h bubbling condition with bubbling condition not under medium pH value time history plot;
Fig. 4 is total hydrogen output comparison diagram under four kinds of conditions;
Fig. 5 produces hydrogen yield and light energy conversion efficiency comparison diagram under four kinds of conditions.
Embodiment
Below in conjunction with the drawings and specific embodiments the present invention is done explanation in further detail.
Utilize ultrasonic wave to improve the method for hydrogen production with photosynthetic bacteria, the photosynthetic bacterium bacterial suspension inoculation that at first will be cultured to logarithmic phase is in the substratum of sterilizing, reinstall in the bubbling style photoproduction reactor, adopt the preface batch culture mode for hydrogen production, drainage is collected hydrogen; In cultivating product hydrogen process, every 3～12h reactor is carried out the Ar bubbling one time, gas flow is 0.2 L/min, each 5 minutes.
Present embodiment adopts the unicellular bacterium of the red vacation in marsh as the object of implementing, and the marsh unicellular Pseudomonas of red vacation is in the Purple Non-sulfur photosynthetic bacterium.
In the substratum of sterilizing, the bubbling style photoproduction reactor of packing into adopts the preface batch culture mode for hydrogen production with the photosynthetic bacterium bacterial suspension inoculation that is cultured to logarithmic phase, drainage is collected gas, every 3h reactor is carried out the Ar bubbling one time, gas flow is 0.2 L/min, each 5 minutes.
Shown in Fig. 1 (a) and Fig. 1 (b), under identical culture condition, bubbling produces considerable influence to the growth metabolism of photosynthetic bacterium: it is higher that 3h advertises under the Ar condition the average cell concentration of photosynthetic bacterium in reactor at interval; The pH value has the trend of rising after reacting mid-term.
Embodiment 2
Every 6h reactor is carried out the Ar bubbling one time, gas flow is 0.2 L/min, each 5 minutes.Photosynthetic bacterial thallus concentration and medium pH value change shown in Fig. 3 (a) and Fig. 3 (b).
Every 12h reactor is carried out the Ar bubbling one time, gas flow is 0.2 L/min, each 5 minutes.Photosynthetic bacterial thallus concentration and medium pH value change shown in Fig. 3 (a) and Fig. 3 (b).
Fig. 4 is a hydrogen production with photosynthetic bacteria total amount comparison diagram under four kinds of conditions, as we can see from the figure, the hydrogen output of the hydrogen production with photosynthetic bacteria amount under the bubbling condition under the bubbling condition not, wherein at interval under the 3h bubbling condition hydrogen output the highest, reach 33 mmol.Fig. 5 is photosynthetic bacterium luminous energy transformation efficiency and a product hydrogen yield comparison diagram under four kinds of conditions, as we can see from the figure, at interval under the 3h bubbling condition light energy conversion efficiency of photosynthetic bacterium and produce the hydrogen yield will be far above the numerical value under the bubbling condition not, light energy conversion efficiency and produce the hydrogen yield and be respectively 18.46 % and 1.79 mol H during 3h at interval wherein
2/ mol glucose.And the light energy conversion efficiency under the bubbling condition and produce the hydrogen yield and be respectively 4.66% and 0.46 mol H not
2/ mol glucose.This explanation Bubbling method can be strengthened hydrogen production with photosynthetic bacteria, improves the hydrogen production with photosynthetic bacteria performance.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from the aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (1)
1. method of utilizing bubbling to improve hydrogen production with photosynthetic bacteria, it is characterized in that: the photosynthetic bacterium bacterial suspension inoculation that will be cultured to logarithmic phase reinstalls in the bubbling style photoproduction reactor in culture medium after sterilization, adopts the preface batch culture mode for hydrogen production; In cultivating product hydrogen process, carry out bubbling one time every 3～12 h, gas flow is 0.2 L/min, each 5 minutes.
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| CN 201110005740 CN102134580A (en) | 2011-01-12 | 2011-01-12 | Method for improving hydrogen production by photosynthetic bacteria using bubbling process |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100578107B1 (en) * | 2004-03-15 | 2006-05-10 | 한국과학기술원 | Fermentative Hydrogen Production from Organic Wastewater Using Gas Sparging and The Fermenting Device |
| CN101089173A (en) * | 2006-06-12 | 2007-12-19 | 河南农业大学 | Hydrogen producing process and apparatus with pig excrement and sewage and photosynthetic bacterium |
| CN101760480A (en) * | 2008-12-25 | 2010-06-30 | 中国科学院上海生命科学研究院 | Bio-hydrogen production method by photosynthetic bacteria |
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2011
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100578107B1 (en) * | 2004-03-15 | 2006-05-10 | 한국과학기술원 | Fermentative Hydrogen Production from Organic Wastewater Using Gas Sparging and The Fermenting Device |
| CN101089173A (en) * | 2006-06-12 | 2007-12-19 | 河南农业大学 | Hydrogen producing process and apparatus with pig excrement and sewage and photosynthetic bacterium |
| CN101760480A (en) * | 2008-12-25 | 2010-06-30 | 中国科学院上海生命科学研究院 | Bio-hydrogen production method by photosynthetic bacteria |
Non-Patent Citations (4)
| Title |
|---|
| 《 International Journal of Hydrogen Energy》 20070409 Halil Berberoglu等 Light transfer in bubble sparged photobioreactors for H2 production and CO2 mitigation 第2273页摘要，第2276页左栏第2段，第2280-2284页第3部分结果与讨论，第4部分结论 1 第32卷, 第13期 * |
| 《International Journal of Hydrogen Energy》 20060329 Dong-Hoon Kim等 Effect of gas sparging on continuous fermentative hydrogen production 2158-2169 1 , * |
| 《中国博士学位论文全文数据库》 20090409 王永忠 固定化光合细菌光生物制氢反应器传输与产氢特性 第28-30页 2.2实验方法与内容 1 , * |
| 《中国博士学位论文全文数据库》 20101019 张川 光合细菌生物膜制氢反应器传输与产氢特性 全文 1 , * |
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Application publication date: 20110727 |