CN102132788A - Method for preparing and applying myxococcus fulvus probiotics for aquaculture - Google Patents

Method for preparing and applying myxococcus fulvus probiotics for aquaculture Download PDF

Info

Publication number
CN102132788A
CN102132788A CN2011100691044A CN201110069104A CN102132788A CN 102132788 A CN102132788 A CN 102132788A CN 2011100691044 A CN2011100691044 A CN 2011100691044A CN 201110069104 A CN201110069104 A CN 201110069104A CN 102132788 A CN102132788 A CN 102132788A
Authority
CN
China
Prior art keywords
days
probiotics
myxococcus fulvus
cultivated
bacterial strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN2011100691044A
Other languages
Chinese (zh)
Other versions
CN102132788B (en
Inventor
贾建波
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Huaiyin Institute of Technology
Original Assignee
Huaiyin Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Huaiyin Institute of Technology filed Critical Huaiyin Institute of Technology
Priority to CN2011100691044A priority Critical patent/CN102132788B/en
Publication of CN102132788A publication Critical patent/CN102132788A/en
Application granted granted Critical
Publication of CN102132788B publication Critical patent/CN102132788B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Landscapes

  • Fodder In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a method for preparing and applying myxococcus fulvus probiotics for aquaculture, which comprises the following steps: (1) taking a myxococcus fulvus JCH-04 the preservation number of which is CGMCC No. 2893, culturing the myxococcus fulvus for 3 days at 30 DEG C in a CY culture medium, and culturing the myxococcus fulvus for 5 days at 30 DEG C in a fermentation medium by shaking at 180rpm (revolutions per minute); (2) taking a circle of strains from a slope, inoculating the strains to a test tube having the CY liquid culture medium and culturing for 3 days at 30 DEG C, and then inoculating the strains to the test tube having the fermentation medium and culturing for 3 days at 30 DEG C; (3) inoculating the liquid strains acquired from the step (2) to a triangular flask having the fermentation medium and culturing for 5 days at 30 DEG C by shaking at 180rpm; and (4) filtering the shaking-cultured strains liquid acquired from the step (3) by using a 0.2 micron microfiltration membrane and concentrating the filtrate into 50 billions CFU (colony-forming unit)/ml strains liquid, thereby acquiring the concentrated strains liquid which is the probiotics. By using the myxococcus fulvus probiotics provided by the invention, the growth of pathogenic microorganism in aquaculture is restrained, the efficiency of feed utilization is increased, and the fatality rate is reduced.

Description

The preparation and the application process of used for aquiculture myxococcus fulvus probiotics
Technical field
The present invention relates to the preparation and the application process of probiotics, be specifically related to a kind of preparation and application process of used for aquiculture myxococcus fulvus probiotics.
Background technology
Slime bacteria as probiotics in the application aspect the culture fishery, its most important characteristic is exactly that slime bacteria is as a kind of predatism microorganism, can engulf Escherichia coli and some other Gram-negative bacterias, the outer active material of the born of the same parents of its secretion has stronger resistance to fungi and bacterium, therefore has the biological control effect of controlling bacterium with bacterium; Slime bacteria can also produce digestive ferment decomposes macromolecular substances such as protein, nucleic acid, ester class, helps the raising of feed digestibility; Slime bacteria and enzyme thereof from bacterium, are not found its illeffects, and from enzyme, chemical composition is a protein, so can not produce to the pollution of environment with to the harm of health.Utilize slime bacteria as feed addictive, will bring into play huge potential aspect disease resistance, the raising aquaculture productivity, and help the production of environmental type aquatic products.
Summary of the invention
The objective of the invention is to: preparation and application process that a kind of used for aquiculture myxococcus fulvus probiotics is provided, the used for aquiculture myxococcus fulvus probiotics pin of the present invention's preparation can suppress the pathogenic microorganism growth in the aquaculture, improve efficiency of feed utilization, reduce case fatality rate.
Technical solution of the present invention is that the preparation method of this used for aquiculture myxococcus fulvus probiotics may further comprise the steps:
(1) bacterial strain extracts: get a strain myxococcus fulvus ( Myxococcus fulvus) JCH-04, preserving number is CGMCC No. 2893, bacterial strain on the CY culture medium 30 ℃ cultivated 3 days, 30 ℃, 180rpm shake bottle and cultivated 5 days in fermentation medium, the GenBank accession number of this bacterial strain 16S rDNA is FJ826620; Described CY culture medium is: casein protein hydrolysate peptone 3g, and yeast extract 1g, calcium chloride 1g, deionized water is fixed molten to 1000mL, pH7.4; Fermentation medium is: soluble starch 10g, analysis for soybean powder 15g, glucose 2.5g, yeast extract 0.5g, CaCl 21g, MgSO 47H 2O 0.5g, deionized water is fixed molten to 1000mL, and pH 7.4;
(2) bacterial strain activation: cultivated 3 days for 30 ℃ from inclined-plane picking one ring bacterial classification inoculation test tube in the CY fluid nutrient medium, will be inoculated in and be equipped with in the fermentation medium test tube 30 ℃ and cultivated 3 days, inoculum concentration is 10 6CFU/mL, 3 times repeatedly;
(3) bacterial strain spreads cultivation: the liquid-spawn inoculation of step (2) 30 ℃, 180rpm in the fermentation medium triangular flask is housed are shaken bottle and cultivated 5 days, and inoculum concentration is 10 6CFU/mL;
(4) bacterial strain collect to concentrate: under aseptic condition, the bottle that shakes of step (3) is cultivated bacterium liquid and is condensed into 50,000,000,000 CFU/mL bacterium liquid with 0.2 μ m micro-filtrate membrane filtration, the concentrated bacterium liquid of acquisition is probiotics.
Wherein, the application process of above-mentioned myxococcus fulvus probiotics: adopt outer spraying low temperature drying to add in the feed, the addition of probiotics is 10 in the aquaculture feed 7-8CFU/g.
The invention has the advantages that: 1, provide myxococcus fulvus ( Myxococcus fulvus) new bacterial strain JCH-04 preserving number is CGMCC No. 2893, the GenBank accession number of this bacterial strain 16S rDNA is FJ826620, it has good cultural character, its zymotic fluid has good antibacterial and multiple enzymolysis ability, can be used as good microbial forage additive and is used for aquaculture field; 2, a strain myxococcus fulvus ( Myxococcus fulvus) JCH-04 is easy to enrichment culture, the preparation zymotechnique is simple to operate, it is carried out toxicological test show and can use safely in animal feed, can effectively suppress the growth of aquaculture pathogenic microorganism, reduces case fatality rate; 3, this bacterial strain can produce amylase and protease, lipase, when the enteron aisle survival of this bacterium animal, just might secrete digestive ferment, and assist digestion improves the digestibility of feed and then raising efficiency of feed utilization; 4, add the myxococcus fulvus probiotics in aquaculture in feed, improve efficiency of feed utilization, the pathogenic microorganism in the described aquaculture is: Escherichia coli ( Escherichia coli), comma bacillus ( Vibri cholera), inferior vibrio parahemolyticus ( Vibrio parahaemolyticus), Aeromonas hydrophila ( Aeromonas hydrophila), pseudomonas aeruginosa ( Pseudomons aeruginosa), flavobacterium aquatile ( Flavobacterium aquatile).
The specific embodiment
Embodiment 1:Bacterial screening and preservation
(1) this bacterial strain is to separate the bacterial strain that pathogenic microorganism is had strong bacteriostasis obtain from soil, with strain culturing, obtain strain cultured solution, observe nutrient solution, measure the ability of the various digestive ferments of product in the nutrient solution simultaneously 6 kinds of pathogenic microorganism bacteriostasis in the aquaculture;
(2) this bacterial strain is according to the GeneBank comparison result, with its called after orange myxobacter JCH-04 subspecies ( Myxococcus fulvusSubsp JCH-04), preserving number is CGMCC No. 2893, and the GenBank accession number of this bacterial strain 16S rDNA is FJ826620.
Embodiment 2:Myxococcus fulvus ( Myxococcus fulvus) JCH-04 is to the pathogenic microorganism bacteriostatic experiment
(1) experiment with the pathogenic microorganism indicator bacteria is: Escherichia coli ( Escherichia coli), inferior vibrio parahemolyticus ( Vibrio parahaemolyticus), comma bacillus ( Vibri cholera), Aeromonas hydrophila ( Aeromonas hydrophila), pseudomonas aeruginosa ( Pseudomons aeruginosa), flavobacterium aquatile ( Flavobacterium aquatile);
(2) culture medium: nutrient agar (MH): beef extract 3g, peptone 10g, NaCl 5g, deionized water is fixed molten to 1000mL, pH7.2-7.4; Salt adding amount is 3% in the cultivation time vibrio parahemolyticus MH culture medium, and cultivating comma bacillus MH pH in culture medium is 8.8, and other is constant, with the MH culture medium; Other indicator bacteria is cultivated with culture medium with the MH culture medium;
(3) experimental technique: indicator bacteria is diluted to different gradients with SPSS, measures OD 600And be coated with flat board, determine the suitableeest coating concentration OD of different bacterium 600Be 0.02-0.03, it is the 9cm planar surface that different indicator bacteria 0.2ml evenly are applied to diameter, dry, at the Oxford cup of the equidistant placement high-temperature sterilization of dull and stereotyped diverse location, will cultivate again concentrate the myxococcus fulvus obtain ( Myxococcus fulvus) JCH-04 bacterium liquid, drawing 200 μ l with aseptic rifle head and be added on the Oxford cup, Oxford cup diameter 5mm cultivated 16-24 hour down at 37 ℃, observed inhibition zone; Antibacterial circle diameter is all greater than 10mm, illustrate myxococcus fulvus ( Myxococcus fulvus) JCH-04 has stronger bacteriostasis to listed 6 kinds of pathogenic microorganism indicator bacterias.
Embodiment 3:Myxococcus fulvus ( Myxococcus fulvus) the JCH-04 safety testing
With myxococcus fulvus ( Myxococcus fulvus) the JCH-04 cultivation, the concentration of regulating bacterium liquid with physiological saline is respectively 10 8, 10 9, 10 10CFU/mL; 24h stops to throw something and feed before on-test, to Penaeus Vannmei (the long 5cm-6cm/ tail of body), adopts the method for injection, dosage is 50 μ L/ tails, uses shrimp 30 tails, does contrast with vibrio parahaemolytious and physiological saline with concentration for every group, establish 6 repetitions for every group, 7d accumulative total death condition observed in record; To young pond crucian carp fish (about body weight 20g/tail), adopt the method that gavages, dosage is the 0.5mL/ tail, and every group of fish 30 tails are done contrast with Aeromonas hydrophila and physiological saline with concentration, establish 6 repetitions for every group, and 20d accumulative total death condition observed in record.
The result shows, injection 10 8, 10 9, 10 10The Penaeus Vannmei of cfu/mL vibrio parahaemolytious is all dead; Gavage 10 8, 10 9, 10 10The young pond crucian carp fish death rate of CFU/mL Aeromonas hydrophila is respectively 89.67%, 97.67%, 100%, the pond crucian carp fish is butchered got liver and spleen, and do electron microscopic section and observe, liver, splenocyte paramophia, characteristics of lesion is obvious; Injection 10 8, 10 9, 10 10CFU/mL Myxococcus fulvusThe Penaeus Vannmei of JCH-04, gavage 10 8, 10 9, 10 10CFU/mL Myxococcus fulvusThe pond crucian carp fish of JCH-04 and blank group are searched for food and are grown all normally at duration of test, do not have dead; The pond crucian carp fish butchered get liver and spleen, do electron microscopic section and observe, liver, splenocyte form are normal, do not see characteristics of lesion.Explanation Myxococcus fulvusThe JCH-04 bacterial strain can be used as the external source probio that has security and potential using value in aquaculture and uses Penaeus Vannmei and pond crucian carp fish safety non-toxic.
Embodiment 4:The preparation of probiotics
(1) bacterial strain extracts: get a strain myxococcus fulvus ( Myxococcus fulvus) JCH-04, preserving number is CGMCC No. 2893, bacterial strain on the CY culture medium 30 ℃ cultivated 3 days, 30 ℃, 180rpm shake bottle and cultivated 5 days in fermentation medium, the GenBank accession number of this bacterial strain 16S rDNA is FJ826620; Described CY culture medium is: casein protein hydrolysate peptone 3g, and yeast extract 1g, calcium chloride 1g, deionized water is fixed molten to 1000mL, pH7.4; Fermentation medium is: soluble starch 10g, analysis for soybean powder 15g, glucose 2.5g, yeast extract 0.5g, CaCl 21g, MgSO 47H 2O 0.5g, deionized water is fixed molten to 1000mL, and pH 7.4;
(2) bacterial strain activation: cultivated 3 days for 30 ℃ from inclined-plane picking one ring bacterial classification inoculation test tube in the CY fluid nutrient medium, will be inoculated in and be equipped with in the fermentation medium test tube 30 ℃ and cultivated 3 days, inoculum concentration is 10 6CFU/mL, 3 times repeatedly;
(3) bacterial strain spreads cultivation: the liquid-spawn inoculation of step (2) 30 ℃, 180rpm in the fermentation medium triangular flask is housed are shaken bottle and cultivated 5 days, and inoculum concentration is 10 6CFU/mL;
(4) bacterial strain collect to concentrate: under aseptic condition, the bottle that shakes of step (3) is cultivated bacterium liquid and is condensed into 50,000,000,000 CFU/mL bacterium liquid with 0.2 μ m micro-filtrate membrane filtration, the concentrated bacterium liquid of acquisition is probiotics.
Embodiment 5:Myxococcus fulvus ( Myxococcus fulvus) the JCH-04 ability of producing various digestive ferments measures
With bottle suspension that shakes that obtains among the embodiment 4, centrifugation, the activity of amylase, protease and lipase in the survey clear liquid.
Amylase activity is 3148 .8U/mL in the mensuration zymotic fluid, and prolease activity is that 78.5 U/mL and lipase activity are 48.4 U/mL; Probio can produce ectoenzyme, is secreted in the surrounding environment, and especially in the enteron aisle of animal, probio is brought into play one of reason of trophism often; This bacterial strain can produce amylase and protease, lipase, therefore, when the enteron aisle survival of this bacterium animal, just might secrete the digestive ferment assist digestion, and then the digestibility of raising feed.
Embodiment 6:Application in the aquaculture feed
Crucian carp feed prescription: fish meal 12%, peanut meal 20%, dregs of beans 15%, soy bean powder 5%, wheat bran 15%, cotton dregs 11%, inferior powder 8%, vitamin premix 0.6%, mineral substance premix 2%, vegetable oil 1.4%.
Prawn feed prescription: dregs of beans 30%, fish meal 32%, inferior powder 15%, ore deposit agent 0.8%, dusty yeast 10%, B B-complex 0.2%.
Microbial inoculum adding method: fish meal is made the particulate material that diameter is 5mm, and the shrimp feed is made diameter 0.5 mm granules, adopts outer spraying method with flush coater, waits bacterium liquid to be immersed in the particulate material low temperature drying again; Fish meal microbial inoculum addition is 10 8CFU/g, shrimp feed addition is 10 7CFU/g.
With the myxococcus fulvus of the present invention preparation ( Myxococcus fulvus) the JCH-04 probiotics adds in the aquatic feeds, experimental results show that through feeding: the feed conversion rate fish reaches 1.84%, and shrimp reaches 1.90%, and the survival rate fish reaches 96.2%, and shrimp reaches 93.0%, and the fish death rate has lowered 27.7%, and the shrimp death rate has lowered 26.5%; Its immune protective rate is also improved; From the finger table reflection of growth performance, the interpolation of microbial inoculum can improve the fishes and shrimps growth performance; Simultaneously digestive ferment index determining in fish, the shrimp body is shown: the interpolation of microbial inoculum can significantly improve fish and shrimp amylase activity, and lipase and proteinase activity are also improved.

Claims (2)

1. the preparation method of used for aquiculture myxococcus fulvus probiotics is characterized in that this preparation method may further comprise the steps:
(1) bacterial strain extracts: get a strain orange myxobacter JCH-04, preserving number is CGMCC No. 2893, bacterial strain on the CY culture medium 30 ℃ cultivated 3 days, 30 ℃, 180rpm shake bottle and cultivated 5 days in fermentation medium, the GenBank accession number of this bacterial strain 16S rDNA is FJ826620; Described CY culture medium is: casein protein hydrolysate peptone 3g, and yeast extract 1g, calcium chloride 1g, deionized water is fixed molten to 1000mL, pH7.4; Fermentation medium is: soluble starch 10g, analysis for soybean powder 15g, glucose 2.5g, yeast extract 0.5g, CaCl 21g, MgSO 47H 2O 0.5g, deionized water is fixed molten to 1000mL, and pH 7.4;
(2) bacterial strain activation: cultivated 3 days for 30 ℃ from inclined-plane picking one ring bacterial classification inoculation test tube in the CY fluid nutrient medium, will be inoculated in and be equipped with in the fermentation medium test tube 30 ℃ and cultivated 3 days, inoculum concentration is 10 6CFU/mL, 3 times repeatedly;
(3) bacterial strain spreads cultivation: the liquid-spawn inoculation of step (2) 30 ℃, 180rpm in the fermentation medium triangular flask is housed are shaken bottle and cultivated 5 days, and inoculum concentration is 10 6CFU/mL;
(4) bacterial strain collect to concentrate: under aseptic condition, the bottle that shakes of step (3) is cultivated bacterium liquid and is condensed into 50,000,000,000 CFU/mL bacterium liquid with 0.2 μ m micro-filtrate membrane filtration, the concentrated bacterium liquid of acquisition is probiotics.
2. the application process of used for aquiculture myxococcus fulvus probiotics, the application process that it is characterized in that this myxococcus fulvus probiotics is: adopt outer spraying low temperature drying to add in the feed, the addition of probiotics is 10 in the aquaculture feed 7-8CFU/g.
CN2011100691044A 2011-03-22 2011-03-22 Method for preparing and applying myxococcus fulvus probiotics for aquaculture Expired - Fee Related CN102132788B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011100691044A CN102132788B (en) 2011-03-22 2011-03-22 Method for preparing and applying myxococcus fulvus probiotics for aquaculture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2011100691044A CN102132788B (en) 2011-03-22 2011-03-22 Method for preparing and applying myxococcus fulvus probiotics for aquaculture

Publications (2)

Publication Number Publication Date
CN102132788A true CN102132788A (en) 2011-07-27
CN102132788B CN102132788B (en) 2012-11-21

Family

ID=44292842

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011100691044A Expired - Fee Related CN102132788B (en) 2011-03-22 2011-03-22 Method for preparing and applying myxococcus fulvus probiotics for aquaculture

Country Status (1)

Country Link
CN (1) CN102132788B (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104789496A (en) * 2015-04-01 2015-07-22 广东省微生物研究所 Application of five myxobacteria to predation of drug-resistance bacteria and preparation of drug-resistance bacteria suppression drugs
CN105648001A (en) * 2016-01-28 2016-06-08 淮阴工学院 Method for extracting mycosubtilin through separation and purification of myxococcus fulvus JCH-04 secondary metabolite
WO2016147121A1 (en) 2015-03-16 2016-09-22 Ecole Polytechnique Federale De Lausanne (Epfl) Archaebacteria in bioactive animal feed, method of making the composition and methods employing the composition
US10668118B2 (en) 2016-03-04 2020-06-02 The Regents Of The University Of California Microbial consortium and uses thereof
US11369644B2 (en) 2018-04-10 2022-06-28 Siolta Therapeutics, Inc. Microbial consortia
US11406675B2 (en) 2019-10-07 2022-08-09 Siolta Therapeutics, Inc. Therapeutic pharmaceutical compositions

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103805542B (en) * 2014-01-23 2016-06-29 广东省微生物研究所 A kind of liquid fermentation method prepared for slime bacteria thalline scale

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1944631A (en) * 2006-09-12 2007-04-11 山东大学 Myxobacteria specific plasmid and its use
CN101638625A (en) * 2009-02-26 2010-02-03 淮阴工学院 Method for culturing orange myxobacter JCH-04 and antibiosis metabolic product

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1944631A (en) * 2006-09-12 2007-04-11 山东大学 Myxobacteria specific plasmid and its use
CN101638625A (en) * 2009-02-26 2010-02-03 淮阴工学院 Method for culturing orange myxobacter JCH-04 and antibiosis metabolic product

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016147121A1 (en) 2015-03-16 2016-09-22 Ecole Polytechnique Federale De Lausanne (Epfl) Archaebacteria in bioactive animal feed, method of making the composition and methods employing the composition
CN104789496A (en) * 2015-04-01 2015-07-22 广东省微生物研究所 Application of five myxobacteria to predation of drug-resistance bacteria and preparation of drug-resistance bacteria suppression drugs
CN107574130A (en) * 2015-04-01 2018-01-12 广东省微生物研究所（广东省微生物分析检测中心） Coral coccus is in predation drug-fast bacteria and is preparing the application in suppressing drug-fast bacteria medicine
CN107574130B (en) * 2015-04-01 2020-07-28 广东省微生物研究所（广东省微生物分析检测中心） Application of coral coccus in predation of drug-resistant bacteria and preparation of drug for inhibiting drug-resistant bacteria
CN105648001A (en) * 2016-01-28 2016-06-08 淮阴工学院 Method for extracting mycosubtilin through separation and purification of myxococcus fulvus JCH-04 secondary metabolite
US10668118B2 (en) 2016-03-04 2020-06-02 The Regents Of The University Of California Microbial consortium and uses thereof
US11033588B2 (en) 2016-03-04 2021-06-15 The Regents Of The University Of California Compositions for treating inflammation and uses thereof
US11369644B2 (en) 2018-04-10 2022-06-28 Siolta Therapeutics, Inc. Microbial consortia
US11406675B2 (en) 2019-10-07 2022-08-09 Siolta Therapeutics, Inc. Therapeutic pharmaceutical compositions

Also Published As

Publication number Publication date
CN102132788B (en) 2012-11-21

Similar Documents

Publication Publication Date Title
CN103403146B (en) For the probiotic bacterium of the biological control for vibrios
CN102132788B (en) Method for preparing and applying myxococcus fulvus probiotics for aquaculture
CN101690545B (en) Method for producing complex micro-ecological preparation with microbial agents and enzyme
CN104195067B (en) One bacillus amyloliquefaciens and the application in aquaculture thereof
CN103275907B (en) Bacillus amyloliquefacien and preparation method and application thereof
JP5872104B2 (en) New Bacillus subtilis {NOVELBACILLUSSUBTILIS}
CN103387948B (en) Application of bacillus safensis in shrimp aquaculture
CN108865953A (en) One plant of wide spectrum inhibits bacillus and its composite bacteria preparation of aquatic products vibrio pathogen
CN103602608B (en) Application of bacillus pumilus in prawn culture
CN103478509B (en) Production method of bacillus amyloliquefaciens antimicrobial lipopeptide and application thereof in prawn feed
KR101536901B1 (en) Probiotics composition for fishes containing a mixture of Bacillus subtilis and phage
CN105238722A (en) Bacillus amyloliquefaciens strain as well as preparation method and application of strain powder preparation of bacillus amyloliquefaciens strain
CN106754551A (en) A kind of bacterium amount lactobacillus preparation high and preparation method and application
CN103045498B (en) Bacillus amyloliquefaciens and application thereof
CN104106726A (en) New probiotic, and probiotic fermentation feed prepared by using it
CN101649298B (en) Bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and application thereof
CN103497907B (en) The application of high-altitude genus bacillus in prawn culturing
CN105176874A (en) Bacillus coagulans fm 603 and application thereof
CN102517227B (en) Enterococcus faecalis and applications and feed additive and leavening agent thereof
CN105175518A (en) Bacteriocin generated by bacillus coagulans FM603 and preparing method thereof
CN106148231B (en) One plant of enterococcus faecalis Y17 and its screening and culturing and application
CN102229902A (en) Bacillus pumilus, feed additive thereof, premix, and feed mildewproof agent
KR20080050506A (en) Additive for animal feed
JP4997500B2 (en) Animal viable agent, feed composition, and method of use
KR102130646B1 (en) Novel lactobacillus salivarius and feed additives for aquacultural fish and crustacea comprising the same

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20121121

Termination date: 20160322