CN102132740A - Lactalbumin thermal denaturation resistant technology and application method thereof in infant formula - Google Patents
Lactalbumin thermal denaturation resistant technology and application method thereof in infant formula Download PDFInfo
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- CN102132740A CN102132740A CN2010101002631A CN201010100263A CN102132740A CN 102132740 A CN102132740 A CN 102132740A CN 2010101002631 A CN2010101002631 A CN 2010101002631A CN 201010100263 A CN201010100263 A CN 201010100263A CN 102132740 A CN102132740 A CN 102132740A
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Abstract
The invention belongs to a production technology of a milk product, and in particular relates to a lactalbumin thermal denaturation resistant technology and an application method thereof in an infant formula. The thermostability of the lactalbumin is increased by changing the external environment of whey-concentrated proteins, and the raw materials for food industry are produced by proportioning the sucrose and the corn oil.
Description
Technical field
The invention belongs to the production technology of dairy products, be specifically related to lactalbumin heat resistanceheat resistant sex change technical method, increase the heat endurance of lactalbumin by the external environment that changes WPC.
Background technology
Lactalbumin is the pure natural protein in the milk, is the key protein source of keeping health.Be fitness and body-building fan, sportsman, need beauty treatment person, gynecological ailments person and very easily tired person's effective nutritional supplementation albumen.Beta lactoglobulin wherein possesses best amino acid ratio, and the branched-amino acid content is high, plays an important role to promoting that protein is synthetic and reduce the protein decomposition, helps fitness enthusiasts to mould graceful build.ALA is the fabulous source of essential amino acid and branched-chain amino acid, also is unique lactalbumin composition that can combine with metallic element and calcium constituent.Nearest research finds that more it may have anti-cancer function.Lactoalbumin is aspect the amino acid proportion structure in addition, and all closely similar with human milk on functional characteristic.Immunoglobulin (Ig) wherein has immunocompetence, can intactly enter proximal small bowel, plays the function of protection mucous membrane of small intestine.
Lactalbumin is fine and close globulin, because amino acid whose composition is different with order, its structure of different types of lactalbumin is also different with order.Because the noncovalent interaction power between protein side chain makes lactalbumin be fine and close chondritic, mainly comprises hydrogen bond, ionic bond and hydrophobic reciprocation.In the structure of protein, what play a major role is hydrophobic reciprocation, protein conformation be formed with the effect that is beneficial to electrostatic attraction and hydrogen bond.The sex change of protein is meant that protein its specific space conformation under some physics and chemical factor effect is changed, thereby causes the change and the bioactive forfeiture of its physicochemical property, and this phenomenon is called protein denaturation.To lactalbumin, it is a kind of to the highstrung protein of heat, needs to alleviate as possible the thermal denaturation degree of lactalbumin in the process of dairy products.The denaturation of protein mainly is because protein molecule inside destructurized.The space structure of native protein is kept by secondary keys such as hydrogen bonds, and secondary key is destroyed after the sex change, and protein molecule just becomes unordered loose stretch-like structure (but primary structure does not change) from the original orderly tight structure that curls.Heating means make protein denaturation, it mainly is the hydrogen bond that destroys in the protein molecule, in change procedure, there are not the fracture and the generation of chemical bond yet, so, originally be in intramolecular hydrophobic grouping and be exposed to molecular surface in a large number, hydrophilic radical then reduces relatively in the distribution on surface, to making the protein particulate can not be molten and lose moisture film with water, be easy to cause intermolecular mutual collision and aggregate and precipitate.This variation is to require in the Dairy Processing technical process to reduce as far as possible, and the real application research of lactalbumin is also fewer.
Summary of the invention
The objective of the invention is at the prior art defective, the method for the little practical application lactalbumin of a kind of albuminous degeneration is provided.
Technical scheme of the present invention is as follows: lactalbumin heat resistanceheat resistant sex change technology and the application process in formula milk comprise the steps:
(1) adopt skimmed whey or full-cream whey, use milipore filter and sodium filter membrane and concentrate, cycles of concentration is the amount of 4 times of raw material wheys, and the content of lactalbumin is 1.2%-3.5%, and operating temperature adopts 15 ℃.
(2) pH of adjustment whey is pH6.2, mixes with milk, corn oil, sucrose.The ratio of milk and condensed whey is 1: 1,2: 1 a relation.The ratio of corn oil and condensed whey is 10: 1, and the ratio of sucrose and condensed whey is 10: 1 relations.Mixed liquor after pre-thermally homogenising, sterilization, concentrating, after concentrated solid reaches 45-49%, spray-drying.
(3) the purification cow's milk of raw milk passes through filtered through gauze, remove impurity, foreign matter, after purifying through milk clarifier again, remove things such as a large amount of newborn mud, the breast epithelial tissue that comes off, leucocyte, reach highly pure purpose, the protein physics and chemistry of raw material milk is between the 2.9%-3.3%, and fat is 3.4%-4.0%, and other indexs such as microorganism meet the national standard of one-level milk.72 ℃ of pasteurizations, 15sec are adopted in sterilization.The standardization of raw milk, in order to obtain the fixedly product of chemical composition, every batch of product must use through standardized raw milk, the raw material butterfat after the standardization and should equal the ratio of fat and non-fat solid in the finished product with the ratio of non-fat solid.Pump into cold boiler after the cow's milk sterilization immediately, it is concentrated to reduce pressure, and removes Ruzhong most of moisture (about 65%, dry matter content brings up to 50% by 12% in the cow's milk, reaches concentrated purpose).When vacuum concentrated the falling film type double effect evaporator that uses the band thermocompressor, first imitates pressure remained on 31～40kPa, and evaporating temperature is 70～72 ℃.Second imitates pressure keeps 16.5～15kPa, and evaporating temperature is 45～50 ℃.When using band thermocompressor falling film type triple effect evaporator, the first effect pressure is 31.9kPa, 70 ℃ of evaporating temperatures, and the second effect pressure is 17.9kPa.Triple effect pressure 9.5kPa, 44 ℃ of evaporating temperatures.Drying process with atomizing is sent into the hot-air that purifies in the hothouse, makes concentrated milk at centrifugal force or pressure simultaneously, under the effect of air-flow, is atomized into countless fine emulsion droplets by atomizer in hothouse, and the moment that contacts with hot-air promptly is dried to powder.
Beneficial effect of the present invention is as follows:
(1) adopt lactalbumin and milk mixed in equal amounts, improved the heat endurance of lactalbumin, to lactalbumin heating contrast, after 80 order filter paper filtered, the heat endurance of lactalbumin improved 80% than original with merely.
(2) adopt after the method condensed whey of ultrafiltration and raw material such as milk mixes, can directly prepare the raw material that various food use, have effect energy-efficient, that enhance productivity.
The specific embodiment
Embodiment 1:(lactalbumin heat resistanceheat resistant sex change technology and the application process in formula milk)
The 100kg raw milk by inspection, purify laggard column criterionization.The purification cow's milk of raw milk is removed impurity, foreign matter by filtered through gauze, after purifying through milk clarifier, removes things such as a large amount of newborn mud, the breast epithelial tissue that comes off, leucocyte again, reaches highly pure purpose, and 72 ℃ of pasteurizations, 15sec are adopted in sterilization.The standardization of raw milk, in order to obtain the fixedly product of chemical composition, every batch of product must use through standardized raw milk, the raw material butterfat after the standardization and should equal the ratio of fat and non-fat solid in the finished product with the ratio of non-fat solid.Adopt hyperfiltration technique to take condensed whey, whey is in advance by degreasing, and fat reaches below 0.5%, 5 times of the volumes that concentrates.The operating pressure of ultrafiltration is 0.05-0.08MPa, and operating temperature is 10 ℃.Use the degreasing condensed whey and mix, adjust pH6.2 with a certain proportion of raw milk.Degreasing condensed whey 100kg mixes with whole milk 100kg.The mixed liquor of cow's milk and whey pumps into cold boiler immediately after sterilization, it is concentrated to reduce pressure, and removes Ruzhong most of moisture (about 65%, dry matter content brings up to 50% by 12% in the cow's milk, reaches concentrated purpose).When using band thermocompressor falling film type triple effect evaporator, the first effect pressure is 31.9kPa, 70 ℃ of evaporating temperatures, and the second effect pressure is 17.9kPa.Triple effect pressure 9.5kPa, 44 ℃ of evaporating temperatures.Drying process with atomizing is sent into the hot-air that purifies in the hothouse, makes concentrated milk at centrifugal force or pressure simultaneously, under the effect of air-flow, is atomized into countless fine emulsion droplets by atomizer in hothouse, and the moment that contacts with hot-air promptly is dried to powder.
Embodiment 2:(lactalbumin heat resistanceheat resistant sex change technology and the application process in formula milk)
Adopt hyperfiltration technique to take condensed whey, whey is in advance by degreasing, and fat reaches below 0.5%, 2 times of the volumes that concentrates.The operating pressure of ultrafiltration is 0.05-0.08MPa, and operating temperature is 10 ℃.The purification cow's milk of raw milk is removed impurity, foreign matter by filtered through gauze, after purifying through milk clarifier, removes things such as a large amount of newborn mud, the breast epithelial tissue that comes off, leucocyte again, reaches highly pure purpose, and 72 ℃ of pasteurizations, 15sec are adopted in sterilization.The standardization of raw milk, in order to obtain the fixedly product of chemical composition, every batch of product must use through standardized raw milk, the raw material butterfat after the standardization and should equal the ratio of fat and non-fat solid in the finished product with the ratio of non-fat solid.Use the degreasing condensed whey and mix, adjust pH6.2 with a certain proportion of raw milk.Degreasing condensed whey 2000kg and whole milk 1000kg mixing 20kg sucrose, 20kg corn oil.Mix after 20 minutes, sterilization pumps into cold boiler then immediately, and it is concentrated to reduce pressure, and removes Ruzhong most of moisture (about 65%, dry matter content brings up to 50% by 12% in the cow's milk, reaches concentrated purpose).When vacuum concentrated the falling film type double effect evaporator that uses the band thermocompressor, first imitates pressure remained on 31～40kPa, and evaporating temperature is 70～72 ℃.Second imitates pressure keeps 16.5～15kPa, and evaporating temperature is 45～50 ℃.
Claims (6)
1. lactalbumin heat resistanceheat resistant sex change technology and the application process in formula milk comprise the steps:
(1) adopt skimmed whey or full-cream whey, use milipore filter and sodium filter membrane and concentrate, cycles of concentration is the amount of 4-6 times of raw material whey, and the content of lactalbumin is 1.2%-3.5%, and operating temperature adopts 15 ℃.
(2) pH of adjustment whey is pH6.2, mixes with milk, corn oil, sucrose.The ratio of milk and condensed whey is 1: 1,2: 1 a relation.
(3) ratio of corn oil and condensed whey is 10: 1, and the ratio of sucrose and condensed whey is 10: 1 relations.Mixed liquor after pre-thermally homogenising, sterilization, concentrating, after concentrated solid reaches 45-49%, spray-drying.
(4) the purification cow's milk of raw milk passes through filtered through gauze, remove impurity, foreign matter, after purifying through milk clarifier again, remove things such as a large amount of newborn mud, the breast epithelial tissue that comes off, leucocyte, reach highly pure purpose, the protein physics and chemistry of raw material milk is between the 2.9%-3.3%, and fat is 3.4%-4.0%, and other indexs such as microorganism meet the national standard of one-level milk.72 ℃ of pasteurizations, 15sec are adopted in sterilization.The standardization of raw milk, in order to obtain the fixedly product of chemical composition, every batch of product must use through standardized raw milk, the raw material butterfat after the standardization and should equal the ratio of fat and non-fat solid in the finished product with the ratio of non-fat solid.Pump into cold boiler after the cow's milk sterilization immediately, it is concentrated to reduce pressure, and removes Ruzhong most of moisture (about 65%, dry matter content brings up to 50% by 12% in the cow's milk, reaches concentrated purpose).When vacuum concentrated the falling film type double effect evaporator that uses the band thermocompressor, first imitates pressure remained on 31～40kPa, and evaporating temperature is 70～72 ℃.Second imitates pressure keeps 16.5～15kPa, and evaporating temperature is 45～50 ℃.When using band thermocompressor falling film type triple effect evaporator, the first effect pressure is 31.9kPa, 70 ℃ of evaporating temperatures, and the second effect pressure is 17.9kPa.Triple effect pressure 9.5kPa, 44 ℃ of evaporating temperatures.Drying process with atomizing is sent into the hot-air that purifies in the hothouse, makes concentrated milk at centrifugal force or pressure simultaneously, under the effect of air-flow, is atomized into countless fine emulsion droplets by atomizer in hothouse, and the moment that contacts with hot-air promptly is dried to powder.
2. lactalbumin heat resistanceheat resistant sex change technology and the application process in formula milk is characterized in that according to claim 1: the milipore filter in the step (1) is selected doughnut PS membrane or rolling milipore filter for use, and molecular cut off is 30Kda (kilodalton).
3. a kind of lactalbumin antitypy technology as claimed in claim 1 and application process in formula milk is characterized in that: to step (2), whey ultrafiltration protein concentrate need mix in proportion with milk or goat milk, yak milk, camel milk etc.
4. a kind of lactalbumin antitypy technology as claimed in claim 1 and application process in formula milk, its feature in: the pH that step (2), whey concentrate need be adjusted it is pH6.2, mixes with milk, corn oil, sucrose.
5. lactalbumin heat resistanceheat resistant sex change technology as claimed in claim 1 and the application process in formula milk, it is characterized in that: to step (2), whey ultrafiltration protein concentrate need mix in proportion with the edible milk of the mankind such as milk or goat milk, yak milk, camel milk, mare's milk.Whey: the milk ratio was from 0.5: 1 to 2: 1.
6. lactalbumin heat resistanceheat resistant sex change technology as claimed in claim 1 and the application process in formula milk, it is characterized in that: to step (3), the ratio of corn oil and sucrose and condensed whey can be adjusted suitable ratio according to the production actual conditions.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103859048A (en) * | 2014-03-13 | 2014-06-18 | 常州毅博生物科技有限公司 | Low-temperature preparation method of high-protein camel milk powder |
| CN104717886A (en) * | 2012-09-28 | 2015-06-17 | 森永乳业株式会社 | Method for manufacturing denatured whey protein |
| CN111743004A (en) * | 2020-07-07 | 2020-10-09 | 甘肃华羚乳品股份有限公司 | Instant whole yak milk powder and preparation method thereof |
-
2010
- 2010-01-25 CN CN2010101002631A patent/CN102132740A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104717886A (en) * | 2012-09-28 | 2015-06-17 | 森永乳业株式会社 | Method for manufacturing denatured whey protein |
| CN104717886B (en) * | 2012-09-28 | 2016-09-14 | 森永乳业株式会社 | The manufacture method of denatured whey protein |
| CN103859048A (en) * | 2014-03-13 | 2014-06-18 | 常州毅博生物科技有限公司 | Low-temperature preparation method of high-protein camel milk powder |
| CN103859048B (en) * | 2014-03-13 | 2015-09-30 | 常州毅博生物科技有限公司 | A kind of low temperature preparation method of high protein camel milk powder |
| CN111743004A (en) * | 2020-07-07 | 2020-10-09 | 甘肃华羚乳品股份有限公司 | Instant whole yak milk powder and preparation method thereof |
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Application publication date: 20110727 |