CN102127599A - Microsatellite marker screening of fast-growing largemouth black basses and application thereof - Google Patents
Microsatellite marker screening of fast-growing largemouth black basses and application thereof Download PDFInfo
- Publication number
- CN102127599A CN102127599A CN 201010605062 CN201010605062A CN102127599A CN 102127599 A CN102127599 A CN 102127599A CN 201010605062 CN201010605062 CN 201010605062 CN 201010605062 A CN201010605062 A CN 201010605062A CN 102127599 A CN102127599 A CN 102127599A
- Authority
- CN
- China
- Prior art keywords
- allelotrope
- micropterus salmoides
- fast
- basses
- glue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses microsatellite marker screening of fast-growing largemouth black basses and application thereof, belongs to the field of fish molecular biology DNA marker technology and application, and particularly relates to microsatellite marker screening of fast-growing largemouth black basses as well as microsatellite marker-aided breeding of fast-growing varieties of largemouth black basses. Five allelomorphic genes closely related to growth traits of largemouth black basses are screened out, and a method for quickly, accurately and effectively detecting fine individuals of largemouth black basses by using molecular markers is established, so that individuals having fine traits can be identified early, thereby providing reference for screening fine parents. By using the method, individuals simultaneously having the five advantageous allelomorphic genes in the production can be selected as parents, fine germplasms can be selected purposefully, thereby ensuring the fineness of the posterior germplasms and improving the growth speed. Compared with the traditional method, the method has the advantages of high purposiveness, direct acting effect, simple operation process, high detection speed and low detection cost, thereby facilitating wide popularization and application.
Description
Technical field
Micropterus salmoides belongs to one of important cultured freshwater fish of China.The invention belongs to fish molecular biology dna marker technology and Application Areas, be specifically related to the Micropterus salmoides microsatellite marker screening of growth fast, and utilize the seed selection work of the auxiliary Micropterus salmoides fast-growth strain of these marks.
Background technology
Micropterus salmoides (
Micropterus salmoidesL.), popular name California perch originates in North America, belongs to Perciformes, Anabantoidei, Sunfishes, sea bass and belongs to, and has that growth is fast, low temperature resistant, a delicious meat and characteristics such as easily fish for, and is one of important cultured freshwater fish.Be generalized to all over the world since the seventies in last century, nineteen eighty-three is introduced a fine variety to Guangdong Province from Taiwan, and all there is breed in national most of area now.2000, the cultured area of Guangdong Province Micropterus salmoides was about 100,000 mu, 6.67 ten thousand tons of output, and the annual production of national Micropterus salmoides is about 100,000 tons.2004, only Guangdong Micropterus salmoides output was 9.48 ten thousand tons, about 12.86 hundred million yuan of the output value.But the Micropterus salmoides of China's introduction is at present formed by the domestic domestication of wild species, lack directive breeding, add to introduce a fine variety and do not pay attention to the working specification that the parent reserves seed for planting and need observe over more than 20 year, even the seed production unit that has is for the convenience on producing, select individual little, the individuality of early sexual maturity is as the parent, the quality that causes China to culture the Micropterus salmoides germplasm at present sharply descends, show that mainly the speed of growth descends, age at sexual maturity generally shifts to an earlier date, the bait transformation efficiency is low, resistibility to environment disadvantageous effect and disease also declines to a great extent, and seriously restricts the development of China's Micropterus salmoides aquaculture.
The speed of growth is to be related to the height that the raiser cultures Micropterus salmoides output, the key of the height of benefit.In recent years, because Micropterus salmoides has trend toward miniaturization, market value also is subjected to very big influence, has influenced Micropterus salmoides culturist's confidence greatly, has restricted the development of Micropterus salmoides aquaculture.The method that improves cultivation of fish grow speed is a lot, feeds as strengthening, and keeps good water quality environment, controls suitable breeding density, selects the high-quality parent population and breeds etc.Wherein, select individual bigger, healthy and strong fish and do breeding and use parent, its offspring may have parent's fine quality such as fast, disease-resistant of growing, but this method is strong not enough to parent's selection purpose, still has much factor and uncertain factor artificially.It is main fish that Micropterus salmoides belongs to carnivorous, grazing eclipse is strong, the bait deficiency is to kill and devour mutually, causes its growth great disparity bigger, only from phenotype judge the largemouth black bass parent quality whether good still be out of true, but select from dna level, can improve the accuracy of selection greatly, and can identify individuality in early days, screen good parent with good character, thereby the shortening breeding cycle is accelerated breeding process.
This laboratory is by research, in the Micropterus salmoides colony that propagates artificially, 40 microsatellite locus are increased, the genotype distributional difference of microsatellite locus in extreme big group of individuals and extremely little group of individuals analyzed in the utilization chi square test, and significant 16 microsatellite locus of selection differences carry out the association analysis of genotype and proterties to the Micropterus salmoides random population.The result shows: association analysis obtains 5 microsatellite locus (JZL60, JZL67, JZL72, MiSaTPW76 and MiSaTPW117) and body weight, body is long and height is remarkable or utmost point significant correlation (P＜0.05 or P＜0.01), simultaneously the not multiple comparisons of isoallele and growth traits is carried out in the site of significant difference, found and body weight, the long the most favourable allelotrope relevant with the height proterties of body is the allelotrope A of JZL60, the allelotrope B of JZL67, JZL72 allelotrope A, the allelotrope B of MiSaTPW76 and the allelotrope B of MiSaTPW117.
This laboratory has the allelic microsatellite marker primer of advantage (JZL60, JZL67, JZL72, MiSaTPW76 and MiSaTPW117) according to 5 that screen, and sets up and identifies the individuality with good character quickly and efficiently.Utilize present method to have this 5 advantages allelic individual reservation in will producing, to improve the variety and quality of Micropterus salmoides.This method is compared with traditional method, and it is strong to have purpose, the direct advantage of action effect.And simple to operate, detect fast, to detect cost low, be convenient to extensively promote the use of.
Summary of the invention
The present invention screens 5 allelotrope that are closely related with the Micropterus salmoides growth traits, and set up a kind of method of utilizing Markers for Detection Micropterus salmoides defect individual fast, accurately and efficiently, to identify individuality in early days, provide reference for screening good parent with good character.
One, microsatellite marker screening
The applicant selects 300 pairs of parent populations in November, 2006 from Nan Shui Micropterus salmoides cultivation base, Shuande, Guangzhou, carry out artificial propagation to the parent population of selecting in April, 2007, raises up seed and culture in same breed pond.Select Micropterus salmoides 160 tails in this breed pond December in the same year, set up the normal distribution of body weight, getting 15% high value individuality is that body weight is above being designated as of 750g " an extreme large group ", 15% low value individuality is that body weight is following being designated as of 315g " an extreme microcommunity ", and each selects 12 tails to be used for preliminary screening with the relevant microsatellite locus of growing at random from two colonies.Select 121 tail Micropterus salmoides to be used for the growth traits association analysis then from identical breed pond at random, this 121 tail Micropterus salmoides is designated as " random population ".
The applicant utilizes that the laboratory has has polymorphism and 40 effective microsatellite markers of somatotype increase to extreme colony, the genotype distributional difference of 40 microsatellite markers in extreme big group of individuals and extremely little group of individuals analyzed in amplification utilization chi square test, and significant 16 the little satellites of selection differences carry out the association analysis of genotype and proterties to the Micropterus salmoides random population.The result shows: association analysis obtains 5 microsatellite locus (JZL60, JZL67, JZL72, MiSaTPW76 and MiSaTPW117) and body weight, body is long and height is remarkable or utmost point significant correlation (P＜0.05 or P＜0.01), simultaneously the not multiple comparisons of isoallele and growth traits is carried out in the site of significant difference, found and body weight, the long the most favourable allelotrope relevant with the height proterties of body is the allelotrope A of JZL60, the allelotrope B of JZL67, JZL72 allelotrope A, the allelotrope B of MiSaTPW76 and the allelotrope B of MiSaTPW117.
Two, the microsatellite marker of screening is used
5 of screening of utilization have the allelic microsatellite marker of advantage, treat the Micropterus salmoides DNA sample of selecting individuality or parent and carry out the PCR detection, amplified production is analyzed with 8% non-denaturing polyacrylamide, to have the allelotrope A(227bp of JZL60 simultaneously), the allelotrope B(262bp of JZL67), JZL72 allelotrope A(202bp), the allelotrope B(257bp of MiSaTPW76) and the allelotrope B(214bp of MiSaTPW117) individuality keep, remove and do not have the allelic individuality of advantage.Utilize present method to have these 5 allelic individualities of advantage simultaneously in will producing and be elected to be the parent, autotelic selection elite germplasm, thus guarantee that offspring's germplasm is good, can improve Micropterus salmoides offspring's the speed of growth greatly.This method is compared with traditional method, and it is strong to have purpose, the direct advantage of action effect.And simple to operate, detect fast, to detect cost low, be convenient to extensively promote the use of.
Three, concrete detection method
(1) micro-satellite primers
(2) extraction of template DNA
(1) after the fin ray of getting fish to be detected organizes 3mg to shred, adds lysate (the 10 mmol/L Tris-HCl of 0.5mL; 0.1 mol/L EDTA; 0.5% SDS; 30mg/L RNase; The 100mg/L Proteinase K, pH8.0), 55 ℃ digested 1 hour, and shook gently frequently therebetween.
(2) add isopyknic phenol/chloroform/primary isoamyl alcohol (25:24:1), put upside down mixing, leave standstill 5 minutes under the room temperature after, 12000 rev/mins centrifugal 10 minutes, get supernatant liquor, use the chloroform extracting more once, after leaving standstill 5 minutes under the room temperature, 12000 rev/mins centrifugal 10 minutes, get supernatant liquor.
(3) dehydrated alcohol of 2 times of volumes of adding, room temperature leaves standstill 10 minutes deposit D NA, and 12000 rev/mins are centrifugal 10 minutes.
(4) with 70% washing with alcohol 1 time, 12000 rev/mins centrifugal 2 minutes, inhale and remove supernatant, room temperature standing and drying 10 minutes adds 50 μ l TE(10mmol/L Tris-HCl; 1mmol/L EDTA, pH8.0) dissolving DNA, 4 ℃ of storages are standby.
(3) PCR reaction system
ddH
2O 14.9μL
10×PCR?Buffer 2.0μL
MgCl
2（25?mmol/L） 0.8μL
4×dNTP（10mmol/L） 0.3μL
TaqEnzyme (5U/ μ L) 0.2 μ L
Upstream and downstream primer (10 μ mol/L) 0.4 μ L
Template DNA (40ng/ μ L) 1.0 μ L
(4) PCR reaction conditions
(5) native polyacrylamide gel electrophoresis detects
(1) 8% non-denaturing polyacrylamide gel preparation (35ml):
| Single water H that steams 2O | 22.259ml |
| 30 ﹪ polyacrylamide stostes | 9.33ml |
| 10×TBE | 3.27ml |
| 20﹪AP | 0.117 ml |
| TEMED | 0.024ml |
Preparation steps:
1. the cleaning of sheet glass: water be stained with lotion the sheet glass scrub after, with ultrapure water flushing, dry standby again.
2. recording of gel: earlier the glass frame that fixes is tilted, gel is poured in the groove of sheet glass lentamente along comb slot edge, plug comb then immediately, allow gel overflow, can avoid producing bubble like this.Add a small amount of single water that steams at glue and comb contact surface, in order to avoid atmospheric oxidation glue surface.When waiting for the glue cohesion, examine to have or not and leak glue or bubble generation.Generally 4-6min promptly begins cohesion behind encapsulating, and it is complete that 25 ℃ of left and right sides 20min of room temperature just can polymerization.When hanging down as room temperature, the corresponding prolongation of polymerization time.
(2) go up sample and gel electrophoresis:
Amplified production and sample-loading buffer behind the 3:1 mixing, are drawn sample on the 4 μ L mixtures with the capillary sample inlet device by volume, and electrophoresis on 8% non-denaturing polyacrylamide gel, electrophoretic buffer are 0.5 * TBE, voltage 230V, electrophoresis 2-3h.
(6) cma staining
(1) electrophoresis finishes glue is taken out, and is put in to fill in single plastic containers (area is bigger slightly than glue) that steam water rinsing 10s gently, removes distilled water;
(2) AgNO of adding 1%
3Solution, jog 3～4 min outwell AgNO on decolorization swinging table
3Solution; With distilled water rinsing twice, be no more than 10s at every turn, outwell distilled water;
(3) add colour developing liquid (NaOH 10g, Na fast
2CO
30.2g, HCHO 2ml, adding distil water is settled to 500ml) 50ml, go behind the vibration 30s;
(4) add 500mL colour developing liquid.In the general 3min, banding pattern manifests;
When (5) treating that banding pattern is clear, the glue taking-up is put in gel becomes phase system to take pictures, and carry out strip analysis.
Beneficial effect
This detection method approximately can be operated in one day and be finished, and can provide detected result fast and accurately for Micropterus salmoides good variety selection and the evaluation that next will carry out.We are by to the allelic evaluation of advantage, be on dna level to the assessment of Micropterus salmoides germplasm quality, purpose is stronger.Detect the required cost of sample with this method and be approximately 0.6 yuan, cost is low, is fit to apply.
Description of drawings
Accompanying drawing is the relevant microsatellite marker screening step of Micropterus salmoides growth.
In addition, with five microsatellite marker sequence tables, annotate: square frame is a primer sequence.
Claims (2)
1. the Micropterus salmoides microsatellite marker of growth screening fast is characterized in that:
Select 300 pairs of parent populations, the parent population of selecting is carried out artificial propagation, raise up seed and in same breed pond, culture, select Micropterus salmoides 160 tails, set up the normal distribution of body weight, getting 15% high value individuality is that body weight is above being designated as of 750g " an extreme large group ", 15% low value individuality is that body weight is following being designated as of 315g " an extreme microcommunity ", each selects 12 tails to be used for preliminary screening with the relevant microsatellite locus of growing at random from two colonies, select 121 tail Micropterus salmoides to be used for the growth traits association analysis then from identical breed pond at random, this 121 tail Micropterus salmoides is designated as " random population ";
Utilize that the laboratory has have polymorphism and 40 effective microsatellite markers of somatotype increase to extreme colony, the genotype distributional difference of 40 microsatellite markers in extreme big group of individuals and extremely little group of individuals analyzed in amplification utilization chi square test, significant 16 the little satellites of selection differences carry out the association analysis of genotype and proterties to the Micropterus salmoides random population, the result shows: association analysis obtains 5 microsatellite locus: JZL60, JZL67, JZL72, MiSaTPW76 and MiSaTPW117, with body weight, body is long and height is remarkable or utmost point significant correlation, as P＜0.05 or P＜0.01, simultaneously the not multiple comparisons of isoallele and growth traits is carried out in the site of significant difference, found and body weight, the long the most favourable allelotrope relevant with the height proterties of body is the allelotrope A of JZL60, the allelotrope B of JZL67, JZL72 allelotrope A, the allelotrope B of MiSaTPW76 and the allelotrope B of MiSaTPW117.
2. the application of the Micropterus salmoides microsatellite marker of growth screening fast is characterized in that:
5 of screening of utilization have the allelic microsatellite marker of advantage, treat the Micropterus salmoides DNA sample of selecting individuality or parent and carry out the PCR detection, amplified production is analyzed with 8% non-denaturing polyacrylamide, the allelotrope A(227bp of JZL60 will be had simultaneously), the allelotrope B(262bp of JZL67), JZL72 allelotrope A(202bp), the allelotrope B(257bp of MiSaTPW76) and the allelotrope B(214bp of MiSaTPW117) individuality keep, remove and do not have the allelic individuality of advantage, utilize present method to have these 5 allelic individualities of advantage simultaneously in will producing and be elected to be the parent, autotelic selection elite germplasm, thereby guarantee that offspring's germplasm is good, can improve Micropterus salmoides offspring's the speed of growth greatly;
Concrete detection method:
(1) micro-satellite primers
(2) extraction of template DNA
(1) after the fin ray of getting fish to be detected organizes 3mg to shred, adds lysate (the 10 mmol/L Tris-HCl of 0.5mL; 0.1 mol/L EDTA; 0.5% SDS; 30mg/L RNase; The 100mg/L Proteinase K, pH8.0), 55 ℃ digested 1 hour, and shook gently frequently therebetween;
(2) add isopyknic phenol/chloroform/primary isoamyl alcohol (25:24:1), put upside down mixing, leave standstill 5 minutes under the room temperature after, 12000 rev/mins centrifugal 10 minutes, get supernatant liquor, use the chloroform extracting more once, after leaving standstill 5 minutes under the room temperature, 12000 rev/mins centrifugal 10 minutes, get supernatant liquor;
(3) dehydrated alcohol of 2 times of volumes of adding, room temperature leaves standstill 10 minutes deposit D NA, and 12000 rev/mins are centrifugal 10 minutes;
(4) with 70% washing with alcohol 1 time, 12000 rev/mins centrifugal 2 minutes, inhale and remove supernatant, room temperature standing and drying 10 minutes adds 50 μ l TE(10mmol/L Tris-HCl; 1mmol/L EDTA, pH8.0) dissolving DNA, 4 ℃ of storages are standby;
(3) PCR reaction system
ddH
2O 14.9μL
10×PCR?Buffer 2.0μL
MgCl
2（25?mmol/L） 0.8μL
4×dNTP（10mmol/L） 0.3μL
TaqEnzyme (5U/ μ L) 0.2 μ L
Upstream and downstream primer (10 μ mol/L) 0.4 μ L
Template DNA (40ng/ μ L) 1.0 μ L
(4) PCR reaction conditions
(5) native polyacrylamide gel electrophoresis detects
(1) 8% non-denaturing polyacrylamide gel preparation (35ml):
Preparation steps:
1. the cleaning of sheet glass: water be stained with lotion the sheet glass scrub after, with ultrapure water flushing, dry standby again;
2. recording of gel: earlier the glass frame that fixes is tilted, gel is poured in the groove of sheet glass lentamente along comb slot edge, plug comb then immediately, allow gel overflow, can avoid producing bubble like this, add a small amount of single water that steams at glue and comb contact surface, in order to avoid atmospheric oxidation glue surface, when waiting for the glue cohesion, examine to have or not and leak glue or bubble produces, generally 4-6min promptly begins cohesion behind encapsulating, and it is complete that 25 ℃ of left and right sides 20min of room temperature just can polymerization, when hanging down as room temperature, the corresponding prolongation of polymerization time;
(2) go up sample and gel electrophoresis:
Amplified production and sample-loading buffer behind the 3:1 mixing, are drawn sample on the 4 μ L mixtures with the capillary sample inlet device by volume, and electrophoresis on 8% non-denaturing polyacrylamide gel, electrophoretic buffer are 0.5 * TBE, voltage 230V, electrophoresis 2-3h;
(6) cma staining
(1) electrophoresis finishes glue is taken out, and is put in to fill in single plastic containers (area is bigger slightly than glue) that steam water rinsing 10s gently, removes distilled water;
(2) AgNO of adding 1%
3Solution, jog 3～4 min outwell AgNO on decolorization swinging table
3Solution; With distilled water rinsing twice, be no more than 10s at every turn, outwell distilled water;
(3) add colour developing liquid (NaOH 10g, Na fast
2CO
30.2g, HCHO 2ml, adding distil water is settled to 500ml) 50ml, go behind the vibration 30s;
(4) add 500mL colour developing liquid, in the general 3min, banding pattern manifests;
When (5) treating that banding pattern is clear, the glue taking-up is put in gel becomes phase system to take pictures, and carry out strip analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010605062 CN102127599A (en) | 2010-12-26 | 2010-12-26 | Microsatellite marker screening of fast-growing largemouth black basses and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010605062 CN102127599A (en) | 2010-12-26 | 2010-12-26 | Microsatellite marker screening of fast-growing largemouth black basses and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102127599A true CN102127599A (en) | 2011-07-20 |
Family
ID=44265869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010605062 Pending CN102127599A (en) | 2010-12-26 | 2010-12-26 | Microsatellite marker screening of fast-growing largemouth black basses and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102127599A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104007161A (en) * | 2014-05-12 | 2014-08-27 | 塔里木大学 | Polyacrylamide gel solution for microsatellite marker polymorphism detection and silver staining method |
| CN104073486A (en) * | 2014-06-23 | 2014-10-01 | 中国水产科学研究院珠江水产研究所 | SNP site related to rapid growth of largemouth black bass as well as identification method and application thereof |
| CN105063031A (en) * | 2015-08-05 | 2015-11-18 | 中国长江三峡集团公司 | Coreius guichenoti microsatellite markers and use thereof |
| CN106701931A (en) * | 2016-12-14 | 2017-05-24 | 中国水产科学研究院珠江水产研究所 | SNP (single nucleotide polymorphism) marker related to rapid growth of micropterus salmoide L. 'excellent bass No. 1' and application of SNP marker |
| CN113637766A (en) * | 2021-07-30 | 2021-11-12 | 中国水产科学研究院珠江水产研究所 | InDel molecular marker related to genetic sex identification of micropterus salmoides and application thereof |
| CN117625802A (en) * | 2023-11-01 | 2024-03-01 | 中国水产科学研究院珠江水产研究所 | Microsatellite marker, primer and application of microsatellite marker |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921850A (en) * | 2010-08-03 | 2010-12-22 | 中国水产科学研究院珠江水产研究所 | Molecular marker screening method of largemouth black bass parent with high hatchability |
-
2010
- 2010-12-26 CN CN 201010605062 patent/CN102127599A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921850A (en) * | 2010-08-03 | 2010-12-22 | 中国水产科学研究院珠江水产研究所 | Molecular marker screening method of largemouth black bass parent with high hatchability |
Non-Patent Citations (4)
| Title |
|---|
| 《2008年中国水产学会学术年会论文摘要集》 20081231 樊佳佳等 大口黑鲈生长性状相关微卫星DNA分子标记的筛选 135 1，2 , * |
| 《分子克隆实验指南第二版》 19921031 J.萨姆布鲁克等著 非变性聚丙烯凝胶电泳 327－330，463－469，672－683 2 , * |
| 《山东农业科学》 20090630 张秀华等 微卫星DNA聚丙烯酰胺凝胶电泳和银染技术的探讨 13－14 2 , 第6期 * |
| 《遗传》 20090531 樊佳佳等 大口黑鲈生长性状的微卫星DNA标记筛选 515_522 1，2 第31卷, 第5期 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104007161A (en) * | 2014-05-12 | 2014-08-27 | 塔里木大学 | Polyacrylamide gel solution for microsatellite marker polymorphism detection and silver staining method |
| CN104073486A (en) * | 2014-06-23 | 2014-10-01 | 中国水产科学研究院珠江水产研究所 | SNP site related to rapid growth of largemouth black bass as well as identification method and application thereof |
| CN104073486B (en) * | 2014-06-23 | 2016-06-08 | 中国水产科学研究院珠江水产研究所 | A kind of SNP site relevant to Micropterus salmoides fast-growth and authentication method thereof and application |
| CN105063031A (en) * | 2015-08-05 | 2015-11-18 | 中国长江三峡集团公司 | Coreius guichenoti microsatellite markers and use thereof |
| CN106701931A (en) * | 2016-12-14 | 2017-05-24 | 中国水产科学研究院珠江水产研究所 | SNP (single nucleotide polymorphism) marker related to rapid growth of micropterus salmoide L. 'excellent bass No. 1' and application of SNP marker |
| CN113637766A (en) * | 2021-07-30 | 2021-11-12 | 中国水产科学研究院珠江水产研究所 | InDel molecular marker related to genetic sex identification of micropterus salmoides and application thereof |
| CN117625802A (en) * | 2023-11-01 | 2024-03-01 | 中国水产科学研究院珠江水产研究所 | Microsatellite marker, primer and application of microsatellite marker |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102242121B (en) | Fast growth gene and parent screening method for micropterus salmoides | |
| CN102127599A (en) | Microsatellite marker screening of fast-growing largemouth black basses and application thereof | |
| CN102286492B (en) | Major gene locus for thousand-grain weight trait of rape and application thereof | |
| CN108315465B (en) | InDel molecular marker closely linked with cowpea salt tolerance related characters, primers and application thereof | |
| CN101921850A (en) | Molecular marker screening method of largemouth black bass parent with high hatchability | |
| CN104805080A (en) | Rapeseed pod number major QTL molecular marker and application thereof | |
| CN106591429B (en) | Method for screening sex reversal genetic control sites of cynoglossus semilaevis, kit and application | |
| CN108179200B (en) | Microsatellite marker linked with procambarus clarkii high fertility character and application thereof | |
| CN105603098A (en) | Microsatellite marker primers used for penaeus monodon microsatellite family identification, identification method and application | |
| CN103409420B (en) | Y-chromosome specific molecular marker of Nile tilapia, and genetic sex determination method and supermale producing method both based on molecular marker | |
| CN105838785B (en) | SSR molecular marker and application with sesame black seed coat gene close linkage | |
| CN106434646A (en) | Four pairs of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeat) primers as well as preparation method thereof and application thereof to construction of fingerprint map of cerasus plants | |
| CN104805179B (en) | A kind of molecular labeling and preparation method and application with cabbage type rape grain re-association | |
| CN109722486B (en) | Watermelon seed navel spot character major gene, molecular marker for detecting major gene and application | |
| CN103525921A (en) | Method for breeding kidding traits by utilizing 3-gene pyramiding effect | |
| CN102242222B (en) | Method for classifying ctenopharyngodon idella based on expressed sequence tag-simple sequence repeats (EST-SSR) marker | |
| CN108456734B (en) | SNP marker related to high alkalinity resistance of litopenaeus vannamei, detection primer and application of SNP marker | |
| CN112626262B (en) | InDel molecular marker linked with radish petal color gene, primer and application thereof | |
| CN102876777B (en) | The special primer of brown croaker EST microsatellite marker and screening method | |
| CN103667272B (en) | The EST-SSR molecule marker relevant to mitten crab growth traits and application | |
| CN101899526B (en) | Method for selecting molecular marker for goat yeaning traits | |
| CN103146697A (en) | Single nucleotide polymorphism of Blackett black cow H-FABP gene and detecting method thereof | |
| CN106916884B (en) | Nile tilapia salt tolerance related molecular marker SSR450 and application thereof | |
| CN110453008B (en) | Molecular marker ZMM6206 closely linked with length and width major gene locus of sesame leaf and application thereof | |
| CN102226189B (en) | Seed number per pod character major gene site of rape and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110720 |