CN102120956A - Shaking table for screening strains - Google Patents
Shaking table for screening strains Download PDFInfo
- Publication number
- CN102120956A CN102120956A CN2010100100622A CN201010010062A CN102120956A CN 102120956 A CN102120956 A CN 102120956A CN 2010100100622 A CN2010100100622 A CN 2010100100622A CN 201010010062 A CN201010010062 A CN 201010010062A CN 102120956 A CN102120956 A CN 102120956A
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- CN
- China
- Prior art keywords
- shaking table
- bacterial strain
- screening
- strains
- shaking
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the screening of strains, particular to a shaking table for screening the strains. A table faceplate and table legs are coated with wear-resistant material layers; and legs fixed on the table faceplate are coated with wear-resistant material layers. A culture vessel is put in the sharking table so as to form a bioreactor provided with multiple tanks connected in parallel; meanwhile, a rotating speed of the shaking table when screening the strains is 50 to 500r/min, and shaking amplitude is 20 to 200mm. The shaking table can be used to rapidly screen excellent strains for production and can improve production efficiency. The shaking table can be applied to fermentation and the like, and the ingredients in the bacteria are fully released by adjusting the speed of the shaking table and reducing dissolved oxygen, and the aging of the bacteria are delayed; meanwhile, material liquid overflow caused by volume increase due to material supplement is avoided. By the shaking table, the strains are stably shaken, so that the radial escape of the conventional shaking table is avoided, and the normal operation of the shaking table is ensured.
Description
Technical field
The present invention relates to the screening of bacterial strain, a kind of specifically shaking table that is used for bacterial strain screening.
Background technology
Existing shaking table to the fermentation aspect, the recent diameter run-out of reciprocating type shaking table of design is obvious, and the utmost point is not steady during work, and primary dcreening operation is used same technical parameter again with multiple sieve, does not do further to inquire into.Also some shaking table is aimed at that ore dressing uses, and the shaking table research that is directed to microorganism culturing seldom.
Summary of the invention
The objective of the invention is to provide a kind of shaking table that is used for bacterial strain screening.
For achieving the above object, the technical solution used in the present invention is:
A kind of shaking table that is used for bacterial strain screening, comprise shaking table head, bed surface and riffling, bed surface panel and riffling are coated with wear-resistant material layer, the fixed riffling also applies high-abrasive material on the bed surface panel, culture vessel is placed the bio-reactor of shaking table and then the parallel connection of formation multiple tank, shaking speed when screening bacterial strain simultaneously: 50～500r/min, shimmy amplitude: 20～200mm.
Every jar of maximum capacity of culture vessel was when described shaking table screened bacterial strain: 40 * 500ml, cage chair size: 144cm * 71.5cm temperature-control range: 5～60 ℃.Described high-abrasive material is made of epoxies material and ceramics powder.
Described epoxies material has the good physics and the chemical reagent of physical strength for having high glossiness, seamless, level and smooth, dustproof, fungi-proofing, moistureproof, impermeable, high strength, acid and alkali-resistance, wear-resisting, shock-resistant.Described epoxies material is that described epoxies material is a Resins, epoxy.
The advantage that the present invention had: adopt shaking table of the present invention can filter out good bacterial classification fast, be used to produce required property, enhance productivity.Shaking table of the present invention can be used for practicalities such as fermentation, regulates shaking table speed, reduces dissolved oxygen amount, and the composition in the thalline is fully discharged, and delays the aging of thalline, avoids simultaneously increasing the feed liquid effusion imagination that causes because of the feed supplement volume.Use shaking table of the present invention can make the bacterial classification vibration steadily, the diameter run-out that existing shaking table do not occur guarantees the shaking table works better.
Embodiment
Shaking table, comprise shaking table head, bed surface and riffling, bed surface panel and riffling are coated with wear-resistant material layer, the fixed riffling also applies high-abrasive material on the bed surface panel, culture vessel is placed the bio-reactor of shaking table and then the parallel connection of formation multiple tank, the vibrational frequency of shaking table is adjustable when screening bacterial strain simultaneously, rotating speed: 50～500r/min, shimmy amplitude: 20～200mm, every jar of maximum capacity is: 40 * 500ml, cage chair size: 144cm * 71.5cm temperature-control range: 5～60 ℃, the temperature consistence: ± 0.5 ℃, accuracy of temperature control: ± 0.5 ℃; Control mode: computer; Timing range: 0～100.0 hour or continuous, power supply: Ac220V, 50Hz, 10A, weight: 500kg
Described high-abrasive material is made of epoxies material and ceramics powder.Described epoxies material be Resins, epoxy available from (Henan epoxy floor material factory), ceramics powder available from (Xingtai far infrared ceramic powder factory)
Adopt above-mentioned shaking table can make bacterium have a good growing environment, and temperature control better, amplitude also is regulatable, common shaking table is bad for temperature controlling, temperature can not keep consistency, the volume ratio of shaking table of the present invention is medium-sized bigger, it is the multiple tank parallel connection, available 40 jars carry out simultaneously, and common shaking table can not reach this capacity, and rotating ratio is very fast, that common only is 50-200r/min, using shaking table of the present invention, to cultivate the quantity of genus bacillus more, genus bacillus then got the 300ml nutrient solution place respectively on above-mentioned shaking table and the common shaking table and cultivate in broth culture, and cultivation results is referring to table 1, table 2.
The different shaking table culturing bacterium of table 1 count results relatively
Attention: numeral * 10 in the table
8CFU/ml.,
Represent mean number ± standard error, p represents in the statistic data a whether significant index of difference, and p＜0.01 expression difference is extremely remarkable.As can be seen from Table 1, in the identical time, compare with the total plate count of common shaking table with the sum of the bacterium of this shaking table, difference is extremely remarkable, and the number of the bacterium of novel shaking table is more.
The shaker fermentation cell count that table 2 is different
As can be known from Table 2, in identical fermentation time, the cell number of the fermentation of novel shaking table is more, has compared significant difference with common shaking table.
Claims (5)
1. shaking table that is used for bacterial strain screening, comprise shaking table head, bed surface and riffling, it is characterized in that: bed surface panel and riffling are coated with wear-resistant material layer, the fixed riffling also applies high-abrasive material on the bed surface panel, culture vessel is placed the bio-reactor of shaking table and then the parallel connection of formation multiple tank, shaking speed when screening bacterial strain simultaneously: 50～500r/min, shimmy amplitude: 20～200mm.
2. by the described shaking table that is used for bacterial strain screening of claim 1, it is characterized in that: every jar of maximum capacity of culture vessel was when described shaking table screened bacterial strain: 40 * 500ml, cage chair size: 144cm * 71.5cm temperature-control range: 5～60 ℃.
3. by the described shaking table that is used for bacterial strain screening of claim 1, it is characterized in that: described high-abrasive material is made of epoxies material and ceramics powder.
4. by the described shaking table that is used for bacterial strain screening of claim 3, it is characterized in that: described epoxies material has the good physics and the chemical reagent of physical strength for having high glossiness, seamless, level and smooth, dustproof, fungi-proofing, moistureproof, impermeable, high strength, acid and alkali-resistance, wear-resisting, shock-resistant.
5. by the described shaking table that is used for bacterial strain screening of claim 4, it is characterized in that: described epoxies material is that described epoxies material is a Resins, epoxy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010100100622A CN102120956A (en) | 2010-01-08 | 2010-01-08 | Shaking table for screening strains |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010100100622A CN102120956A (en) | 2010-01-08 | 2010-01-08 | Shaking table for screening strains |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102120956A true CN102120956A (en) | 2011-07-13 |
Family
ID=44249624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010100100622A Pending CN102120956A (en) | 2010-01-08 | 2010-01-08 | Shaking table for screening strains |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102120956A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2137561Y (en) * | 1991-09-08 | 1993-07-07 | 平桂矿务局机械厂矿山设备服务公司 | Shanking table with double curved-corrugation riffling |
| CN1384187A (en) * | 2001-04-27 | 2002-12-11 | 华东师范大学 | Batch culture process of variant strain of Bacillus subtilis |
| CN100396382C (en) * | 2004-12-23 | 2008-06-25 | 李家林 | Durable highly efficient concentrating table and its manufacturing method |
-
2010
- 2010-01-08 CN CN2010100100622A patent/CN102120956A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2137561Y (en) * | 1991-09-08 | 1993-07-07 | 平桂矿务局机械厂矿山设备服务公司 | Shanking table with double curved-corrugation riffling |
| CN1384187A (en) * | 2001-04-27 | 2002-12-11 | 华东师范大学 | Batch culture process of variant strain of Bacillus subtilis |
| CN100396382C (en) * | 2004-12-23 | 2008-06-25 | 李家林 | Durable highly efficient concentrating table and its manufacturing method |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110713 |