CN102115487A - Method for preparing polypeptide by taking seashells as raw material - Google Patents
Method for preparing polypeptide by taking seashells as raw material Download PDFInfo
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- CN102115487A CN102115487A CN2010105729895A CN201010572989A CN102115487A CN 102115487 A CN102115487 A CN 102115487A CN 2010105729895 A CN2010105729895 A CN 2010105729895A CN 201010572989 A CN201010572989 A CN 201010572989A CN 102115487 A CN102115487 A CN 102115487A
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- shell
- polypeptide
- acid
- feedstock production
- solid
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- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
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Landscapes
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a method for preparing polypeptide by taking seashells as a raw material. The method comprises the following steps of: (1) cleaning the seashells with a cleaning solution and drying and grinding at normal temperature or at the temperature between 40 DEG C and 200 DEG C to obtain seashell powder; (2) soaking the seashell powder in an alkali solution for 10-120 hours and treating with ultrasonic waves or microwaves for 5-300 minutes to obtain a solid-liquid mixture with the liquid to solid ratio between 2 ml/g and 100 ml/g; (3) filtering the solid-liquid mixture to obtain a filtrate; and (4) extracting the filtrate with an organic solvent, separating and purifying to obtain the polypeptide. The method for preparing the polypeptide by taking the seashells as the raw material has the advantages of simple technology and mild operating condition. The prepared product has potential application in the development of products such as an antibacterial polypeptide medicament, an agricultural fungicide, a pesticide, a preservative, a surface glazing agent and the like.
Description
Technical field
The present invention relates to shell and utilize the field, particularly a kind of is the method for feedstock production polypeptide with the shell.
Background technology
Shellfish is the principal item of China's sea farming, accounts for more than 70% of national sea farming ultimate production, and ultimate production reaches 1,200 ten thousand tons/year, and the last 60% that accounts for Gross World Product ranks first in the world.
The fast development of shellfish culture has produced a large amount of shell wastes.For a long time, a large amount of shell resources fail to be fully used, and are poured into seashore, sandy beach as waste, have caused serious littoral zone to pollute.Shell pollutes and to have become the environmental problem that the coastland needs to be resolved hurrily, and it is more and more serious to pile up the environmental problem that is caused year by year.One of bottleneck that shell handling problem becoming restriction shellfish culture industry further develops, the resource utilization development and use of shell waste are imperative.
When communicable disease produces gradually existing medicine and develops when resistance, that ocean environment then provides is antimycotic, the new method of parasite, bacterium and viral disease.Exploration and development that nearly half a century is experienced in marine drug research have obtained many valuable experience accumulation and abundant research data.There is more than 1.8 ten thousand kilometer long shoreline in China, about 5,000,000 square kilometres of oceanic area, and the ocean medicine resource is contained very abundant.A large amount of development research achievement has strengthened the confidence that people develop marine drug, and development prospect is wide.
Use a large amount of sterilant and agricultural chemicals in the process of growth of agricultural-food, in the course of processing, use synthetic preservation agent and surperficial lustering agent, mostly be organic compound in these materials greatly with phenyl ring, heterocycle structure, organosulfur structure, organic heavy metal structure, long-term application, residual fairly obvious in soil and fruit.Now objectionable impurities such as agricultural chemicals to the pollution of vegetable and fruit and other food serious threat to human existence and health.Therefore, use natural matter, exploitation sterilant, agricultural chemicals, preservation agent and surperficial lustering agent non-chemically synthetic, that can enter the mouth to seem very urgent.
In recent years, various biological utilisation antibacterial peptides are as intrinsic defense system, and the viewpoint that is used for resisting microbiological attack gains public acceptance.Antibacterial peptide is the micromolecule polypeptide of being made up of 10～40 amino acid that all kinds of biologies are generally expressed, be referred to as " totipotency peptide ", bacterium, fungi, virus, tumour cell, cancer solid tumor and protozoon all there are inhibition or killing action, have has a broad antifungal spectrum, thermostability is strong, molecular weight is little and characteristics such as non-immunogenicity, its bactericidal mechanism uniqueness, be difficult for producing resistant organism, be expected to be developed as peptide antibiotics of new generation.To the mankind, all biologies can both produce antibacterial peptide from plant, insect.It as in the organism timely and effectively, nonspecific defensive barrier, have the ability that opposing is infected.
Antibacterial peptide is strong as a class antibacterial ability, has a broad antifungal spectrum, many, the available scopes of kind are wide, the functional peptides of target bacterial strain Xiao Yi generation resistant mutation, will have broad application prospects aspect medicine, cosmetic, biology feed additive, antiseptics for natural food and the animal and plant disease resisting genetically engineered.
At present, shell research mainly concentrates on chemical ingredients, the aspects such as formation mechanism, constitutional features and biomimetic material of shell, and is less to the pharmaceutical use research of shell.
Shell is important Chinese medicinal materials, can treat multiple disease, as being the effect that Sea-ear Shell that raw material is made has flat liver to make eye bright with abalone shells, cures mainly diseases such as liver wind is dizzy, optic atrophy; The inner casing of cuttlefish can be treated wound, insane disease, heart trouble and stomach trouble, and can be used to hemostasis; The clam shell can cure mainly hemoptysis, haematemesis, and the function of removing heat-phlegm is arranged, and can treat chronic tracheitis, lymphoid tuberculosis, gastric and duodenal ulcer; Red spiral shell shell can be treated stomachache; The giant clam shell has calmness, calms the nerves, the toxicide effect; With the tortoiseshell is that the tortoiseshell that raw material is made looses, and is a kind of externally applied agent, controls and festers, and anti-inflammatory analgesic effect etc. is arranged.
The composite minerals that shell is made up of nano-calcium carbonate and biomacromolecule, typical shell structure can be divided into stratum corneum, prismatic layer and nacreous layer.Stratum corneum is positioned at the outermost layer of shell, is a kind of of scleroprotein, for the calcareous crystalline deposition of carbonic acid provides framework, and can the acid proof corrosion.Prismatic layer is between nacreous layer and stratum corneum, and the prismatic layer of fresh water shell is generally the aragonite phase, and inorganic in the seawater shell prismatic layer is the calcite phase mutually.Stratum corneum and prismatic layer can only be formed by the secretion of mantle dorsal edge.Nacreous layer is positioned at the shell innermost layer, and inorganic is the aragonite phase mutually.Form by pallial full surface secretion, and thicken, be rich in gloss along with the growth of shellfish.Though shell is of a great variety, come in every shape, the color difference, chemical constitution is similar, mainly contains the lime carbonate and a spot of conchiolin (claiming conchiolin again, is the mixture that is formed by multiple organic substrate) that account for full shell 95%.Different sorts shell or same kind shell be in varying environment, different growing stages, and different shells, its organic substrate all has difference, but all is mainly protein and polysaccharide.
Protein wherein contains people's physical efficiency synthetic and can not synthetic amino acid, and as glycine, L-Ala, arginine etc., the angle glutelin becomes amino acid through acid hydrolysis, and major part can participate in the metabolism of human body enzyme system.Lime carbonate can neutralize with hydrochloric acid in gastric juice, calcium ion can make the former formation thiozell of the thiozell in the blood and make blood coagulation, and calcium ion enters the vigor that people's physical efficiency promotes ATP enzyme in the human body cell, regulates the blood acid-basicity, very useful to HUMAN HEALTH, especially can delay senility.The arresting convulsion of calming the nerves, heat-clearing benefit the moon, the detoxifcation that makes eye bright, anti-inflammatory promote the production of body fluid pearl powder, the function of cough-relieving apophlegmatic medically having, be applicable to diseases such as gastric and duodenal ulcer, insomnia, neurasthenia, hepatitis, swelling and pain in the throat, hypertension, epilepsy, rheumatic heart disease etc. there are certain curative effect, have now made LIUSHEN WAN, Xiaoer Huichun Dan, compound asthma kind of patent medicine surplus in the of 20 such as loose.Pearl powder can be made senior invigorants such as pearl wine, pearl white fungus, and pearl cosmetic oil, makeup such as pearl perfume, in addition, existing people's report is with the agent of shell system calcium replenishing, is that raw material is developed into function of human body and mixes with the ocean shell, become the solubility ocean calcium of left-handed structure, its performance is similar to pearl powder, and human body is 64.3% to the specific absorption of its calcium, and is more much higher than common pearl powder (specific absorption is 29%).
A large amount of data show, protein in the shell is feature to be rich in Gly, Asx (Asp+Asn), Glx (Glu+Gln) and Ser, in different sorts and the same kind different structure shell, Gly has higher content, following characteristics are totally arranged: acidic amino acid content is higher in the 1. solvable organic matter (SM), be rich in hydrophilic radical, wherein especially the highest with the Asx content x in the calcite shell, can reach on 40%, acidic amino acid content x in the aragonite shell is relatively low, but all be all tart polyanionic protein, may be from membranin.2. soluble organic matter (IM) is compared with SM and is rich in hydrophobic grouping, the alkalescence that becomes, and its Asx+Glx content is more much lower than SM, and Gly, Val, Ala and Lys content x increase, and belong to the silk-protein proteinoid.
Owing to technical reason is directly measured difficulty of proteinic aminoacid sequence, can only be measured to the amino acid length about 20 usually, brought very big difficulty for studying proteinic 26S Proteasome Structure and Function.Therefore can only infer indirectly.Be antiparallel β-laminated structure after for example Worms infers SM according to infrared spectroscopic study and calcium combines, be coiled structure at random when not combining with calcium.Can obtain more complete protein amino acid sequence by modern molecular biology, make people more deep understanding arranged the organic matter in the mollusk shell.
Modern molecular biology studies show that the protein in the shell usually is multi-functional protein.The GS territory of the bright albumin A among for example red abalone Haliotis rufescens has the plasticity function, makes macromole tool ductility, gives the mechanical property of nacreous layer excellence; C territory (C
1-C
10) be rich in the Cys residue, this territory is by reaching intermolecular self-assembly in the disulfide linkage may command protein molecule; The alkalescence territory can interact with other anion-containing macromole (as acid SM): C end territory is the territory of proteinoid enzyme inhibitors, but the protein component in its protective shell exempts from degraded.Contain maximum acidic domain D-1 of amino-acid residue and D-2 among the SM in the calcite shell (MSP-1) and can contain Ca
2+, to the significance that is formed with of shell, its alkaline territory and GS territory may have same function with alkaline territory and the GS territory in the bright albumen.Acid Gly-X-Asn:Gly-X-Asn duplicate domain (x=Asp, Asn or Glu) in the pearl protein in the Pictada fucata nacreous layer can be in conjunction with Ca
2+The function of class carbonic anhydrase territory (CA territory) tool carbonic anhydrase, but the following reaction of its catalysis: H
2O+CO
2→ HCO
3 -+ H
+, the formation that promptly can be shell provides carbanion.
In addition, the aminoacid sequence of 13 poly-(richness) Ala territories of being rich among the insoluble organic MSI60 in the pinctada fucata Pinctada fucata nacreous layer and the constructional feature in 39 poly-Gly territories of richness and spider silk fibroin is closely similar, thereby has crystalline antiparallel β-laminated structure.And the insoluble organic MSI30 in the calcite layer of same kind does not have poly-(richness) Ala territory, but contains poly-Gly territory, and it participates in the formation of β-laminated structure equally.N-end and C-end all contain the Cys residue in two kinds of protein, and it may form disulfide linkage at intramolecularly (with intermolecular), participates in the self-assembly of molecule.
Antimicrobial peptides such as mussel defensin mainly by its hemocyte produce, store, secrete, activity such as transhipment and anti-sterilization.At present the defensin of finding be small molecule amount, positively charged ion mostly, be rich in halfcystine, alpha-helix, the active small peptide of the anti-specified microorganisms of tool.The mussel defensin of having been illustrated is divided into 4 classes: (1) mussel defensin (MDA and MDB) and Mediterranean Sea mussel defensin (MGD1 and MGD2): (2) Myticins (Myticin A and Myticin B): (3) Mytilins according to half total in its primary structure peptide propylhomoserin sequence; (4) Mytimycin.
Separate the defensin from edible mussel M.edulis blood plasma, one is named as mussel defence A (Mytilusdefensin A is abbreviated as MDA), and another is named as mussel defensin B (Mytilus defensin B is abbreviated as MDB).The molecular weight of MDA is 4314.3Da, contains 37 amino-acid residues, comprising 6 cysteine residues (Cys-), constitutes 3 intramolecular disulfide bonds.The molecular weight of MDB is 4392.4Da, and 35 amino-acid residues are arranged, and also contains 6 cysteine residues.Have a few extra residue at its COOH-end, similar with MDA.
Mediterranean Sea mussel defensin is to separate from Mediterranean Sea mussel (defensin of Mytilus galloprovimcialis blood plasma and hemocyte or defensin sample peptide, be Mytilus galloprovimcialis defensein, be abbreviated as MGDs, it is divided into MGD1 and MGD2.MGDs is an original newcomer of arthropods defensin family.The MGD1 molecular weight is 4kD, and at first the acid acellular hemolymph supernatant liquor of being found from the Mediterranean Sea mussel in 1996 by Hubert etc. also separated obtaining afterwards in the fragment of the rich organoid from hemocyte.
MGD1 contains 39 amino-acid residues.On the 39th glycine residue is arranged, C-holds amidation.MGD1 is present in the fragment that is rich in organoid of hemocyte.The MGD2 initial separation also measured the mRNA of MGD2 afterwards from the Mediterranean Sea mussel in edible mussel.The MGD2 molecule contains 39 amino-acid residues and 8 Cys-, compares with the aminoacid sequence of MGD1, and 6 different amino-acid residues are arranged.
1999, Mitta etc. discretely in extra large mussel hemocyte and blood plasma draw the antimicrobial peptide of the new rich halfcystine of a class, i.e. Myticin.Its total two isoform of A, B.Myticin A molecular weight is 4.43kD (4437.28 scholar 2.46) Da, contains 40 residues, comprises 8 cysteine residues or 4 disulfide linkage.Myticin B molecular weight is (4563.45 scholar 1.32) Da, and 8 cysteine residues are arranged.Part N-terminal sequence obtains to comprise 7 residues of 1 alkyl halfcystine.
The Mytilins molecule is rich in half moon bright propylhomoserin (8 Cys-), totally 34 residues, and molecular weight is about 3877.79Da.The three-dimensional structure height constraint of Mytilins, the connection of the disulfide linkage in the primary structure and halfcystine array are different from the antimicrobial peptide of the rich halfcystine of known so far arthropods, the frog, Mammals or plant.
The about 22 μ M of the concentration of Mytilins in mussel blood, this is the MIC of most bacteriums of trying.Their anti-G
-The activity of bacterium is better than anti-G
+Bacterium.Mytilin comprises 5 isoforms, and they are respectively Mytilin A, Mytilin B, Mytilin C, Mytilin D and Mytilin G1.
Mytimvcin separates certainly without pathogeny evoked edible mussel blood plasma, and molecular weight is 6.3KD, and 32 residues are arranged, and comprises 12 Cys-.Mytimycin is special antimycotic mussel peptide, has obtained to contain the NH of 32 residues
2The partial sequence of-end, it is equivalent to half of complete sequence approximately.In the protein database, do not search any homologous peptide is arranged therewith.This peptide has the retardation effect to some fungi growth.
In addition, Jung in 2005 etc. obtain AOD from the oyster gill, molecular weight is 4205.0Da, form by 38 amino acid, contain 6 halfcystines, to the MIC that removes from office blue formula positive bacteria Lactococcus lactis subsp.actis and Staphylococcus aureus is respectively 2.4 and 3.0 μ g/mL, is 7.6 and 15.0 μ g/mL to the MIC that removes from office blue formula negative bacterium Escherichia coli D31 and Vihrio parahemolyticus.This is to find antibacterial peptide first from this bivalve shellfish of oyster.
At present the source mainly is to extract from the human body of shellfish or blood for the antimicrobial polypeptide of shellfish, almost less than about the research from the antimicrobial polypeptide of shellfish housing.The proteinic structure in the shellfish shell and the structure of antimicrobial polypeptide have resemblance, as all being rich in amino acid such as L-Ala, Xie Ansuan and glycine, constitutional featuress such as beta sheet are arranged all.According to structures shape character, in conjunction with the preliminary study in early stage, find that the treatment solution after the discarded shell comprehensive utilization has the effect of mould fungus inhibition growth, contain antimicrobial substance in the indication treatment solution, mainly be protein or its degraded product that the shell the inside extracts.
The method that protein, polypeptide extraction separation are used always comprises: salting-out process, ultrafiltration process, gel filtration method, isoelectric point precipitation, ion exchange chromatography, affinity chromatography, adsorption chromatography, CCD, enzymolysis process etc.These methods usually are grouped together specific material are carried out separation and purification, and above-mentioned these methods also are means commonly used during albumen, polypeptides matter are analyzed simultaneously, as chromatography, electrophoresis etc.Amino acid composition analysis, amino acid sequence analysis, field desorptiion mass spectrum, IR, UV spectrum, CD, circle and chromatogram, biological detection method, labelled with radioisotope method and immunological method etc. have been applied to all that the result of polypeptides matter identifies, among the analyzing and testing.Above-mentioned sophisticated protein, the extraction of polypeptide, separation, purification technique are available.
Patent " the antiseptic-germicide that multiple shell mixed sintering forms, sur-face peeling agent and application thereof " (publication number is: CN 101116449A) disclose the antiseptic-germicide that the multiple shell mixed sintering of a kind of usefulness forms, the sur-face peeling agent, select oyster for use, clam, clam, haliotis diversicolor Reeve, Mytilus crassitesta Lischke, at least two kinds of shells mix in Octopus and the scallop, at the electric furnace internal heating, outlet temperature is 600 ℃～1200 ℃, through firing simultaneously, obtaining granular size after the pulverizing is 0.1 μ m～150 μ m, median size is the antiseptic-germicide of 2 μ m～60 μ m, the sur-face peeling agent has than the better bacteriostatic action of prior art, has effects such as the agricultural-food remained on surface agricultural chemicals of removal and lustering agent.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, providing a kind of is the method for feedstock production polypeptide with the shell.
The step of method that with the shell is the feedstock production polypeptide is as follows:
1) shell is cleaned with scavenging solution, normal temperature down or 40～200 ℃ of dryings, pulverizing obtains the shell powder;
2), and, obtain the solid-liquid mixture that liquid-solid ratio is 2～100ml/g with ultrasonic wave or microwave treatment 5～300 minutes with oyster shell whiting body and function aqueous slkali soaking 10～120 hours;
3) the solid-liquid mixture filtration is obtained filtered liquid;
4) filtered liquid obtains polypeptide with organic solvent extraction, separation, purifying.
Described shell is Mytilus crassitesta Lischke shell, Mytilus edulis shell, pearl shell, scallop shell, CONCHA MERETRICID SEU CYCLINAE, freshwater mussel shell or calf oyster shell.Described scavenging solution is that quality hundred is than the dilute hydrochloric acid, dilute sulphuric acid, rare nitric acid, dilute acetic acid, rare lactic acid or sodium hydroxide, clorox or the aqueous hydrogen peroxide solution that are 0.1～10%.The granularity of described shell powder is 2～10000 orders.Described alkaline solution is that quality hundred is than the sodium hydroxide solution or the potassium hydroxide solution that are 0.1～10%.Described organic solvent is acetone, glycerine, ethylene glycol, methyl alcohol, ethanol, b propanol, n-propyl alcohol, aceticanhydride, propyl carbinol, methylethylketone, acetic acid penta fat or vinyl acetic monomer.
Advantage of the present invention is the operational condition gentleness, and technology is simple, and facility investment and energy consumption are little; Raw material sources are extensive, and are cheap; The product of preparation has higher potential market and is worth, and can be widely used in having good economic and social benefit in the product developments such as antimicrobial polypeptide medicine and disinfectant use in agriculture, agricultural chemicals, preservation agent and surperficial lustering agent.
Embodiment
The present invention is further described in conjunction with following example, but content of the present invention is not limited only to content related among the embodiment.
Embodiment 1:
1) shell is cleaned with 10% dilute hydrochloric acid, dry at normal temperatures, be crushed to 2 orders;
2) sodium hydroxide solution with the oyster shell whiting body and function 0.1% pulverized soaks 10 hours (extracting conchiolin matter), and liquid-solid ratio is 100ml/g (L/Kg), and with ultrasonication 300 minutes;
3) above-mentioned solid-liquid mixture is filtered to get filtrate;
4) filtered liquid obtains polypeptide with acetone extraction, separation, purifying.
Embodiment 2:
1) shell is cleaned up with 0.1% dilute sulphuric acid, then descend drying, be crushed to 10000 orders at 200 ℃;
2) potassium hydroxide solution with the oyster shell whiting body and function 10% pulverized soaks 120 hours (extracting conchiolin matter), and liquid-solid ratio is 2ml/g (L/Kg), and with microwave treatment 300 minutes;
3) above-mentioned solid-liquid mixture is filtered to get filtrate;
4) filtered liquid obtains polypeptide with extraction using alcohol, separation, purifying.
Embodiment 3:
1) shell is cleaned up with 2% rare nitric acid, then descend drying, be crushed to 100 orders at 80 ℃;
2) potassium hydroxide solution with the oyster shell whiting body and function 2% pulverized soaks 24 hours (extracting conchiolin matter), and liquid-solid ratio is 10ml/g (L/Kg), and with ultrasonication 60 minutes;
3) above-mentioned solid-liquid mixture is filtered to get filtrate;
4) filtered liquid obtains polypeptide with b propanol extraction, separation, purifying.
Embodiment 4:
1) shell is cleaned up with 3% rare nitric acid, then descend drying, be crushed to 200 orders at 100 ℃;
2) sodium hydroxide solution with the oyster shell whiting body and function 3% pulverized soaks 48 hours (extracting conchiolin matter), and liquid-solid ratio is 30ml/g (L/Kg), and with microwave treatment 120 minutes;
3) above-mentioned solid-liquid mixture is filtered to get filtrate;
4) filtered liquid obtains polypeptide with n-propyl alcohol extraction, separation, purifying.
Embodiment 5:
1) shell is cleaned up with 5% dilute acetic acid, then descend drying, be crushed to 2000 orders at 120 ℃;
2) potassium hydroxide solution with the oyster shell whiting body and function 5% pulverized soaks 72 hours (extracting conchiolin matter), and liquid-solid ratio is 60ml/g (L/Kg), and with microwave treatment 180 minutes;
3) above-mentioned solid-liquid mixture is filtered to get filtrate;
4) filtered liquid obtains polypeptide with n-butanol extraction, separation, purifying.
Embodiment 6:
1) shell is cleaned up with 7% superoxol, then 160 ℃ dry down, be crushed to 5000 orders;
2) potassium hydroxide solution with the oyster shell whiting body and function 7% pulverized soaks 96 hours (extracting conchiolin matter), and liquid-solid ratio is 90ml/g (L/Kg), and with ultrasonication 240 minutes;
3) above-mentioned solid-liquid mixture is filtered to get filtrate;
4) extraction of filtered liquid spent glycol, separation, purifying obtain polypeptide.
Claims (6)
1. one kind is the method for feedstock production polypeptide with the shell, it is characterized in that its step is as follows:
1) shell is cleaned with scavenging solution, normal temperature down or 40～200 ℃ of dryings, pulverizing obtains the shell powder;
2), and, obtain the solid-liquid mixture that liquid-solid ratio is 2～100ml/g with ultrasonic wave or microwave treatment 5～300 minutes with oyster shell whiting body and function aqueous slkali soaking 10～120 hours;
3) the solid-liquid mixture filtration is obtained filtered liquid;
4) filtered liquid obtains polypeptide with organic solvent extraction, separation, purifying.
2. as claimed in claim 1 a kind of be the method for feedstock production polypeptide with the shell, it is characterized in that described shell is Mytilus crassitesta Lischke shell, Mytilus edulis shell, pearl shell, scallop shell, CONCHA MERETRICID SEU CYCLINAE, freshwater mussel shell or calf oyster shell.
3. as claimed in claim 1 a kind of be the method for feedstock production polypeptide with the shell, it is characterized in that described scavenging solution is that quality hundred is than the dilute hydrochloric acid, dilute sulphuric acid, rare nitric acid, dilute acetic acid, rare lactic acid or sodium hydroxide, clorox or the aqueous hydrogen peroxide solution that are 0.1～10%.
4. as claimed in claim 1 a kind of be the method for feedstock production polypeptide with the shell, the granularity that it is characterized in that described shell powder is 2～10000 orders.
5. as claimed in claim 1 a kind of be the method for feedstock production polypeptide with the shell, it is characterized in that described alkaline solution is that quality hundred is than the sodium hydroxide solution or the potassium hydroxide solution that are 0.1～10%.
6. as claimed in claim 1 a kind of be the method for feedstock production polypeptide with the shell, it is characterized in that described organic solvent is acetone, glycerine, ethylene glycol, methyl alcohol, ethanol, b propanol, n-propyl alcohol, aceticanhydride, propyl carbinol, methylethylketone, acetic acid penta fat or vinyl acetic monomer.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604433A (en) * | 2012-02-14 | 2012-07-25 | 浙江大学 | Preparation method of aldehyde modified shell micro powder |
| CN104026652A (en) * | 2014-02-28 | 2014-09-10 | 国家海洋局第三海洋研究所 | Preparing method of food grade marine-organism-sourced shell micro powder |
| CN104525963A (en) * | 2014-12-16 | 2015-04-22 | 国家海洋局第三海洋研究所 | Method for preparing shell-loaded nano-silver composite antibacterial material with assistance of microwaves |
| CN105854823A (en) * | 2016-04-27 | 2016-08-17 | 贝壳派创新科技（深圳）有限公司 | Biological composite antibacterial adsorption material and preparation method thereof |
| CN107213168A (en) * | 2017-06-27 | 2017-09-29 | 贝壳派创新科技（深圳）有限公司 | Skin disease nursing for treating product based on marine organism extract and preparation method thereof |
| CN107557414A (en) * | 2017-10-11 | 2018-01-09 | 广西南宁荣威德新能源科技有限公司 | A kind of extracting method of shell active material |
| CN111109297A (en) * | 2019-12-31 | 2020-05-08 | 上海海洋大学 | Application of shell powder of mytilus coruscus in bacteriostasis of marine bacteria |
| CN112877389A (en) * | 2021-01-20 | 2021-06-01 | 广州市尚梓化工科技有限公司 | Preparation method of pearl bright white peptide and application of pearl bright white peptide in whitening cosmetics |
| CN114921400A (en) * | 2022-05-26 | 2022-08-19 | 中国海洋大学 | Method for culturing primary blood cells of Mytilus edulis |
| CN115140751A (en) * | 2022-07-29 | 2022-10-04 | 山东理工大学 | Preparation method of high-purity aluminum hydroxide |
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2010
- 2010-11-30 CN CN2010105729895A patent/CN102115487A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604433A (en) * | 2012-02-14 | 2012-07-25 | 浙江大学 | Preparation method of aldehyde modified shell micro powder |
| CN104026652A (en) * | 2014-02-28 | 2014-09-10 | 国家海洋局第三海洋研究所 | Preparing method of food grade marine-organism-sourced shell micro powder |
| CN104525963A (en) * | 2014-12-16 | 2015-04-22 | 国家海洋局第三海洋研究所 | Method for preparing shell-loaded nano-silver composite antibacterial material with assistance of microwaves |
| CN105854823A (en) * | 2016-04-27 | 2016-08-17 | 贝壳派创新科技（深圳）有限公司 | Biological composite antibacterial adsorption material and preparation method thereof |
| CN107213168A (en) * | 2017-06-27 | 2017-09-29 | 贝壳派创新科技（深圳）有限公司 | Skin disease nursing for treating product based on marine organism extract and preparation method thereof |
| CN107557414A (en) * | 2017-10-11 | 2018-01-09 | 广西南宁荣威德新能源科技有限公司 | A kind of extracting method of shell active material |
| CN111109297A (en) * | 2019-12-31 | 2020-05-08 | 上海海洋大学 | Application of shell powder of mytilus coruscus in bacteriostasis of marine bacteria |
| CN112877389A (en) * | 2021-01-20 | 2021-06-01 | 广州市尚梓化工科技有限公司 | Preparation method of pearl bright white peptide and application of pearl bright white peptide in whitening cosmetics |
| CN114921400A (en) * | 2022-05-26 | 2022-08-19 | 中国海洋大学 | Method for culturing primary blood cells of Mytilus edulis |
| CN115140751A (en) * | 2022-07-29 | 2022-10-04 | 山东理工大学 | Preparation method of high-purity aluminum hydroxide |
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Application publication date: 20110706 |