CN102101894A - Preparation method of chitooligosaccharide hydrolyzate - Google Patents
Preparation method of chitooligosaccharide hydrolyzate Download PDFInfo
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- CN102101894A CN102101894A CN2009102636519A CN200910263651A CN102101894A CN 102101894 A CN102101894 A CN 102101894A CN 2009102636519 A CN2009102636519 A CN 2009102636519A CN 200910263651 A CN200910263651 A CN 200910263651A CN 102101894 A CN102101894 A CN 102101894A
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- hydrolyzed solution
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- chitin oligosaccharide
- hydrogen peroxide
- chitosan
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Abstract
The invention discloses a preparation method of a chitooligosaccharide hydrolyzate, which is characterized by comprising the following steps: taking chitosan of which the deacetylation degree is higher than 80% as a raw material, adding hydrogen peroxide, adding a catalyst, reacting at a certain temperature for 30-60 minutes, and finally, removing the residual hydrogen peroxide to obtain the brown chitooligosaccharide hydrolyzate.
Description
Affiliated technical field
The present invention relates to a kind of preparation method of chitin oligosaccharide hydrolyzed solution, belong to the fine chemical technology field.
Background technology:
Chitin oligosaccharide has unique physiologically active and functional property, as regulates immunity, promotes the growth, antitumor, antibiotic of probiotics; Fungicidal promotes plant-growth; Soluble in water, water absorbability and moisture retention arranged; Be a kind of emerging functional oligose, have been widely used in fields such as food, medicine, agricultural and makeup.The preparation of chitin oligosaccharide hydrolyzed solution has a lot of methods, and the most frequently used is exactly chemical method, and acid degradation method and oxidation degradation method are arranged in the chemical method, oxidation degradation method long reaction time commonly used, and also oxidation and degraded can take place in the reduction end group of hydrolysate, and by product is many.The invention provides a kind of preparation method of chitin oligosaccharide hydrolyzed solution, the reaction times only needs dozens of minutes, and hydrolysis is thorough, and hydrolysates to stabilize is convenient to control, the residual harmful advantage that becomes to grade not.
Summary of the invention
The present invention provides a kind of preparation method of chitin oligosaccharide hydrolyzed solution; it is characterized in that with the chitosan of degree of deacetylation more than 80% be raw material; add hydrogen peroxide; add catalyzer again; reacted at a certain temperature 30-60 minute; remove remaining hydrogen peroxide at last, obtain brown chitin oligosaccharide hydrolyzed solution.
Technical scheme:
The present invention provides a kind of preparation method of chitin oligosaccharide hydrolyzed solution, it is characterized in that the chitosan of degree of deacetylation more than 80% is raw material, adds hydrogen peroxide, add catalyzer again, stirring reaction 30-60 minute at a certain temperature, when temperature reaches fusing point, the rapid hydrolysis of chitosan; After cooling, add Ca (OH)
2Add solid catalyst again, remove remaining hydrogen peroxide at last, obtain brown chitin oligosaccharide hydrolyzed solution.
The invention has the advantages that:
The preparation method of a kind of chitin oligosaccharide hydrolyzed solution provided by the invention, the reaction times only needs dozens of minutes, and hydrolysis is thorough, and the chitin oligosaccharide hydrolyzed solution is stable, is convenient to control, not the residual harmful advantage that becomes to grade.
Embodiment:
Enforcement of the present invention is like this: getting the chitosan of degree of deacetylation more than 80% is raw material, adds the hydrogen peroxide of 5-15%, adds 1% catalyzer again, temperature of reaction 75-95 degree centigrade, and constantly stirring reaction 30-60 minute.When temperature reaches melting temperature, the rapid hydrolysis of chitosan; The cooling back adds Ca (OH) in hydrolyzed solution
2, add solid catalyst again, removing remaining hydrogen peroxide is zero, filters and eliminates solid catalyst, obtains brown chitin oligosaccharide hydrolyzed solution.
Claims (5)
1. the invention provides a kind of preparation method of chitin oligosaccharide hydrolyzed solution, it is characterized in that getting the chitosan of degree of deacetylation more than 80% is raw material, adds the 5-15% hydrogen peroxide, adds 1% catalyzer again, temperature of reaction 75-95 degree centigrade, constantly stirring reaction 30-60 minute; When temperature reaches melting temperature, the rapid hydrolysis of chitosan; The cooling back adds Ca (OH) in hydrolyzed solution
2, add solid catalyst again, removing remaining hydrogen peroxide is zero, filters and eliminates solid catalyst, obtains brown chitin oligosaccharide hydrolyzed solution.
2. the preparation method of a kind of chitin oligosaccharide hydrolyzed solution as claimed in claim 1, the degree of deacetylation that it is characterized in that chitosan does not contain heavy metal ion more than 80%, white or canescence drying products.
3. the preparation method of a kind of chitin oligosaccharide hydrolyzed solution as claimed in claim 1 is characterized in that the concentration of hydrogen peroxide 5-15% of dissolving raw material, analytical pure.
4. the preparation method of a kind of chitin oligosaccharide hydrolyzed solution as claimed in claim 1 is characterized in that controlled temperature 75-95 degree centigrade, time 30-60 minute, treats the appearance of fusing point temperature, termination reaction.
5. the preparation method of a kind of chitin oligosaccharide hydrolyzed solution as claimed in claim 1, it is characterized in that hydrolyzed solution cooling after, add Ca (OH) earlier
2Remove the oxalic acid that hydrolysis produces, adding solid catalyst again, to remove remaining hydrogen peroxide be zero, and the solids removed by filtration catalyzer obtains brown chitin oligosaccharide hydrolyzed solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102636519A CN102101894A (en) | 2009-12-18 | 2009-12-18 | Preparation method of chitooligosaccharide hydrolyzate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102636519A CN102101894A (en) | 2009-12-18 | 2009-12-18 | Preparation method of chitooligosaccharide hydrolyzate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102101894A true CN102101894A (en) | 2011-06-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102636519A Pending CN102101894A (en) | 2009-12-18 | 2009-12-18 | Preparation method of chitooligosaccharide hydrolyzate |
Country Status (1)
| Country | Link |
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| CN (1) | CN102101894A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114438150A (en) * | 2022-04-11 | 2022-05-06 | 中国海洋大学 | Method for inhibiting browning of chitosan oligosaccharide |
-
2009
- 2009-12-18 CN CN2009102636519A patent/CN102101894A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114438150A (en) * | 2022-04-11 | 2022-05-06 | 中国海洋大学 | Method for inhibiting browning of chitosan oligosaccharide |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110622 |