CN102100585B - Uterine horn embryo-flushing needle suitable for sheep - Google Patents
Uterine horn embryo-flushing needle suitable for sheep Download PDFInfo
- Publication number
- CN102100585B CN102100585B CN 201110064000 CN201110064000A CN102100585B CN 102100585 B CN102100585 B CN 102100585B CN 201110064000 CN201110064000 CN 201110064000 CN 201110064000 A CN201110064000 A CN 201110064000A CN 102100585 B CN102100585 B CN 102100585B
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- Prior art keywords
- tube
- needle
- embryo
- sheep
- needle tubing
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Links
- 241001494479 Pecora Species 0.000 title claims abstract description 27
- 238000011010 flushing procedure Methods 0.000 title claims abstract description 21
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 25
- 238000011084 recovery Methods 0.000 claims abstract description 7
- 210000005239 tubule Anatomy 0.000 claims abstract description 4
- 210000004291 uterus Anatomy 0.000 claims description 19
- 239000007788 liquid Substances 0.000 abstract description 9
- 210000002257 embryonic structure Anatomy 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 239000000741 silica gel Substances 0.000 description 7
- 229910002027 silica gel Inorganic materials 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 238000005406 washing Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 229920003023 plastic Polymers 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 210000003101 oviduct Anatomy 0.000 description 2
- 238000002161 passivation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241000283707 Capra Species 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 244000137852 Petrea volubilis Species 0.000 description 1
- 206010042573 Superovulation Diseases 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000472 morula Anatomy 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Surgical Instruments (AREA)
Abstract
The invention discloses a uterine horn embryo-flushing needle suitable for sheep, which is used for recovering sheep uterine horn embryos. The uterine horn embryo-flushing needle comprises a needle tube, a connection tube and a tail tube, wherein the needle tube is a hollow tube, the front part of the needle tube is a bevel needle point, and the middle part of the needle tube is provided with a strip-shaped hole; the front part of the tail tube is a tubule, and the rear part of the tail tube is a horn-shaped opening; and the connection tube is a strip-shaped flexible hollow tube, one end of the connection tube is connected with the rear part of the needle tube, and the other end of the connection tube is connected with the front part of the tail tube. The uterine horn embryo-flushing needle also comprises a filter which is connected with the tail tube. The outside diameter of the needle tube of the embryo-flushing needle is small, so that the embryo-flushing needle causes small damages to the inner membrane of the sheep uterine horn; and the liquid yielding quantity of the strip-shaped hole on the needle tube is high, so that the embryo recovery is smooth.
Description
[technical field]
The present invention relates to the animal embryo transplanting tool, relate in particular to a kind of uterine horn embryo-flushing needle that is suitable for sheep, mainly is to the recovery of the early stage morula/blastaea of sheep cornua uteri when reclaiming for embryo in the superovulation sheep body.
[background technology]
In Embryo Transfer in Goat, reclaim late uterus embryo of stage of development and be conducive to improve the rear pregnancy rates of transplanting.To the embryo who enter cornua uteri be reclaimed, will wash cornua uteri, be about to injection needle from fallopian tube and the little crook of cornua uteri inserting needle, inject embryo washing water towards the large curved direction of cornua uteri, reclaim embryo washing water from the uterine horn embryo-flushing needle tail end, under stereomicroscope, pick the embryo.
At present, because the complete embryo of domestic shortage reclaims apparatus, majority all with high price from external introduction embryo recovery tube, wherein sheep uterine horn embryo-flushing pipe is a plastic flexible pipe, be made of inside and outside two-layer, internal layer is vacuum, and front end has two little liquid outlets, at liquid outlet two openings are arranged: one is the embryo washing water outlet, and one connects the integral body that syringe can inwardly inject the installation composition of air.Because the minimum outer diameter of plastic flexible pipe is generally about 2.0～4mm, caliber is thicker, will sheep uterine horn embryo-flushing pipe could be inserted the uterus with mosquito forceps in the hole of a diameter 2cm of uterus bundle first during use, and is therefore larger to the vulneratio uteri of sheep, easily hemorrhage, be difficult to recover.And its liquid outlet is circular port, and the aperture is less, easily blocks during use.In addition, this product is disposable product, and cost is higher, has hindered to a certain extent the commercial application of sheep embryo transfer technology.
[summary of the invention]
The technical problem to be solved in the present invention provides a kind of damage that can reduce sheep cornua uteri inner membrance, so that embryo's recovery makes things convenient for smooth and easy and can reusable uterine horn embryo-flushing needle.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is, a kind of uterine horn embryo-flushing needle that is suitable for sheep, be used for sheep cornua uteri embryo's recovery, comprise needle tubing, connection tube and tail pipe, needle tubing is hollow pipe, and the external diameter of needle tubing is 1.0～1.6mm, the front portion is beveled point, and the needle tubing middle part has a bar hole; The tail pipe front portion is tubule, and the rear portion is horn opening; Connection tube is the flexible hollow tube of strip, and an end of connection tube is connected with the needle tubing rear portion; The other end of connection tube is connected with the front portion of tail pipe; Also comprise filter, filter is connected with tail pipe.
Preferably, bar hole be length along the linear hole of needle tubing axis, the smooth of the edge in hole is without burr.
Preferably, bar hole is that the smooth of the edge in hole is without burr around the spiral-shaped orifices of needle tubing wall rotation.
Preferably, the optimum length of spiral-shaped orifices is one week of tube wall around needle tubing.
Preferably, filter is that metal is made, and filter comprises 90-100 purpose copper mesh filter disc.
Preferably, filter is that plastics are made, and it is the mesh of 150～160 μ m that filter has the aperture.
Preferably, filter and tail pipe are threaded connection.
Preferably, filter is connected by buckle with tail pipe.
Preferably, beveled point is through Passivation Treatment.
The invention has the beneficial effects as follows, the external diameter that rushes embryo pin needle tubing is less, and little to the damage of donor sheep cornua uteri inner membrance, the liquid outlet quantity of bar hole is large on the needle tubing, and the embryo reclaims smooth and easy.
[description of drawings]
The present invention is further detailed explanation below in conjunction with the drawings and specific embodiments.
Fig. 1 is the structural representation that the present invention is suitable for the uterine horn embryo-flushing needle embodiment 1 of sheep.
Fig. 2 is the needle tubing partial enlarged drawing that the present invention is suitable for the uterine horn embryo-flushing needle embodiment 1 of sheep.
Fig. 3 is the A place partial enlarged drawing of Fig. 1.
Fig. 4 is the structural representation that the present invention is suitable for the uterine horn embryo-flushing needle embodiment 2 of sheep.
Fig. 5 is the B place partial enlarged drawing of Fig. 3.
Fig. 6 is the needle tubing partial enlarged drawing that the present invention is suitable for the uterine horn embryo-flushing needle embodiment 3 of sheep.
Among the figure, 1-needle tubing, 101-bar hole, 2-silica gel hose, 3-tail pipe, 4-filter.
[specific embodiment]
Embodiment 1:
Fig. 1 is a kind of uterine horn embryo-flushing needle that is suitable for sheep, comprises needle tubing 1, flexible pipe 2 and tail pipe 3,
Needle tubing 1 is the circular hollow pipe of 1.0mm for external diameter, the front portion is beveled point, beveled point is through Passivation Treatment, the smooth of the edge and very not sharp keen, have a bar hole 101 at the middle part of needle tubing 1, bar hole is that its length is around one week of needle tubing wall (Fig. 2) around the spiral-shaped orifices of needle tubing wall rotation, the width of bar hole is 1mm, and the smooth of the edge of bar hole is without burr.The front portion of tail pipe 3 is tubules, and the rear portion is horn opening.Silica gel hose is flexible hollow pipe, and an end of silica gel hose is enclosed within the rear portion of needle tubing 1; The other end of silica gel hose is enclosed within the front portion of tail pipe 3.
The present embodiment only need be avoided blood vessel insertion gently and just can enter cavity of uterus owing to having selected the needle tubing of more small-bore (1.0mm) when advancing to rush the embryo pin, need not to prick the hole in the uterus, and is minimum to the sheep vulneratio uteri.In addition, have spiral bar hole at needle tubing, hole shape is larger, the liquid outlet quantity when having guaranteed to use, and because the length of spiral-shaped orifices around one week of needle tubing, is difficult for occuring choking phenomenon during use.
Among Fig. 3, filter made of plastic 4 is housed also on the tail pipe 3, the edge of filter 4 is connected by buckle with tail pipe 3 edges, and filter 4 is provided with filter screen, and the aperture of filter screen is 150 μ m.Because sheep embryo diameter is generally in the scope of 115～135 μ m, filter screen can allow the sheep embryo be collected by outflow smoothly, some larger impurity and large uterine epithelial cell sheet are then blocked and can not flow out by filter screen in the cavity of uterus, can so that search the embryo, can also play further bacteriological filtration effect simultaneously like this.For ease of filter gauze cleaning, filter 4 can be pulled down and load onto from tail pipe 3 easily.For filtering better, dual filter screen can be set to increase filter effect.
During use, syringe needle from fallopian tube and the little crook of cornua uteri inserting needle, is injected embryo washing water towards the large curved direction of cornua uteri, the tail pipe recovery embryo washing water from uterine horn embryo-flushing needle picks the embryo under stereomicroscope.
Embodiment 2:
In the present embodiment, needle tubing 1 directly adopts syringe needle transformation for animals to make (Fig. 4) with tail pipe 3, and filter 4 usefulness metals are made, and are provided with 90-100 purpose copper mesh filter disc, and filter 4 is connected (Fig. 5) by screw thread with tail pipe 3.All the other structures are identical with embodiment 1.
Manufacture method: select long 6～7cm, the veterinary needle head of external diameter 1.0～1.2mm, to connect first the injector end joint blocks with file, again metal injection pin beveled end blunt, its front end is made screw-like liquid outlet (width of screw thread approximately 1mm is advisable), screw-like liquid outlet edge will polish smooth without burr with file and sand paper processing, the length of threaded portion is 1.5～2.5cm), overlap the silica gel tube that connects with its internal diameter that suits mutually in the rear end, silica gel tube length 15～25cm, be used for the backflow rush ovum liquid, again silica gel tube is terminal connect injection needle to clip needle end after remaining pintle part.
Embodiment 3:
In Fig. 6, have rectilinear bar hole at needle tubing 1, more convenient during fabrication and processing.All the other structures are with embodiment 1.
Below described by reference to the accompanying drawings three kinds of embodiments of the present invention, but those skilled in the art can make various distortion or modification within the scope of the appended claims.
Claims (2)
1. a uterine horn embryo-flushing needle that is suitable for sheep is used for sheep cornua uteri embryo's recovery, comprises needle tubing, connection tube and tail pipe, and described needle tubing is hollow pipe, and the external diameter of needle tubing is 1.0～1.6mm, and the front portion is beveled point; Described tail pipe front portion is tubule, and the rear portion is horn opening; Described connection tube is the flexible hollow tube of strip, and an end of connection tube is connected with the needle tubing rear portion; The other end of connection tube is connected with the front portion of tail pipe; Also comprise filter, described filter is connected with tail pipe, it is characterized in that, described needle tubing middle part has a bar hole; Described bar hole is that the smooth of the edge in hole is without burr around the spiral-shaped orifices of needle tubing wall rotation.
2. the uterine horn embryo-flushing needle that is suitable for sheep according to claim 1 is characterized in that, the optimum length of described spiral-shaped orifices is one week of tube wall around needle tubing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110064000 CN102100585B (en) | 2011-03-17 | 2011-03-17 | Uterine horn embryo-flushing needle suitable for sheep |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110064000 CN102100585B (en) | 2011-03-17 | 2011-03-17 | Uterine horn embryo-flushing needle suitable for sheep |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102100585A CN102100585A (en) | 2011-06-22 |
| CN102100585B true CN102100585B (en) | 2013-04-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110064000 Expired - Fee Related CN102100585B (en) | 2011-03-17 | 2011-03-17 | Uterine horn embryo-flushing needle suitable for sheep |
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| Country | Link |
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| CN (1) | CN102100585B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676377A (en) * | 2012-03-12 | 2012-09-19 | 中国人民解放军兰州军区乌鲁木齐总医院 | Novel external embryo (ovum) collector |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201046152Y (en) * | 2006-09-28 | 2008-04-16 | 尼普洛株式会社 | Device for picking oosphere |
| CN201537153U (en) * | 2009-11-20 | 2010-08-04 | 新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心 | Mouth-sucking embryo picking sleeve |
| CN101797189A (en) * | 2010-02-23 | 2010-08-11 | 湖北省农业科学院畜牧兽医研究所 | Apparatus for collecting early-stage embryo or early-stage oocyte of pig and collecting method |
| CN201595916U (en) * | 2010-02-10 | 2010-10-06 | 江西诺德医疗器械有限公司 | Disposable dual-cavity egg taking needle |
| CN202027748U (en) * | 2011-03-17 | 2011-11-09 | 安徽农业大学 | Uterine horn embryo flushing needle suitable for sheep |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2202502Y (en) * | 1994-09-26 | 1995-07-05 | 郭德田 | Manual spiral type cutting and suction device for lumbar vertebra |
| JP4299746B2 (en) * | 2004-08-17 | 2009-07-22 | 株式会社北里サプライ | Percutaneous egg collection device |
| CN201094640Y (en) * | 2007-08-22 | 2008-08-06 | 王学雷 | Tumor tissue examination sampling needle |
| CN201631882U (en) * | 2010-03-04 | 2010-11-17 | 杨佐明 | Drainage tube |
-
2011
- 2011-03-17 CN CN 201110064000 patent/CN102100585B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201046152Y (en) * | 2006-09-28 | 2008-04-16 | 尼普洛株式会社 | Device for picking oosphere |
| CN201537153U (en) * | 2009-11-20 | 2010-08-04 | 新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心 | Mouth-sucking embryo picking sleeve |
| CN201595916U (en) * | 2010-02-10 | 2010-10-06 | 江西诺德医疗器械有限公司 | Disposable dual-cavity egg taking needle |
| CN101797189A (en) * | 2010-02-23 | 2010-08-11 | 湖北省农业科学院畜牧兽医研究所 | Apparatus for collecting early-stage embryo or early-stage oocyte of pig and collecting method |
| CN202027748U (en) * | 2011-03-17 | 2011-11-09 | 安徽农业大学 | Uterine horn embryo flushing needle suitable for sheep |
Non-Patent Citations (1)
| Title |
|---|
| JP特开2006-55219A 2006.03.02 |
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| CN102100585A (en) | 2011-06-22 |
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