CN102095705A - Laser scanning confocal microscope-based hair imaging method - Google Patents
Laser scanning confocal microscope-based hair imaging method Download PDFInfo
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- CN102095705A CN102095705A CN 201010584822 CN201010584822A CN102095705A CN 102095705 A CN102095705 A CN 102095705A CN 201010584822 CN201010584822 CN 201010584822 CN 201010584822 A CN201010584822 A CN 201010584822A CN 102095705 A CN102095705 A CN 102095705A
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- laser scanning
- hairline
- focusing microscope
- laser
- scanning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8444—Fibrous material
Abstract
The invention provides a laser scanning confocal microscope-based hair imaging method, which is characterized by comprising the following steps of: marking hairs by using 0.5 percent acridine orange, and washing the hairs by using deionized water for 1 minute; and scanning a hair sample by using 488nm-wavelength laser of an argon ion laser of a laser scanning confocal microscope, and acquiring an image by using an XYZ scanning program. The method is easy to operate and the flaky structure of the hairs can be observed.
Description
Technical field
The invention provides a kind of hairline formation method, belong to the microscopic study technical field based on laser scanning co-focusing microscope.
Background technology
Laser scanning co-focusing microscope is made of microscope optical system, LASER Light Source, scanister and computer operating system, a whole set of instrument is by computer control, and the operation between each parts is switched and all can be carried out easily and flexibly in the computer operation platform interface.Laser scanning co-focusing microscope adopts laser as light source, detecting pinhole before utilization is placed on the illumination pin hole behind the light source and is placed on detecting device realizes that point throws light on and point is surveyed, the light of launching by the illumination pin hole from the light of light source focuses on certain point of sample focal plane, this institute's emitted fluorescence is imaged on the detecting pinhole, and the emission light beyond this point is detected pin hole to be stopped.Illumination pin hole and detecting pinhole are to illuminated point or to be detected a little be conjugation, so to be detected a little be confocal point, and the plane that is detected a place is confocal plane.
Use Laser Scanning Confocal Microscope, can gather tissue or cell interior the fluorescence labeling signal, observe the morphological change and the inner microtexture of variation, tissues observed or the cell of the interior ion concentration of living cells.Simultaneously, can also carry out the semi-quantitative analysis of three-dimensionalreconstruction and fluorescence labeling intensity to the image of gathering; Fluorescence in situ hybridization, the assignment of genes gene mapping and original position PCR in real time product are analyzed.At present, laser scanning co-focusing microscope has become the important means of cell biology, Neuscience, pharmacology, histological anatomy research.
Ordinary optical microscope can not be used for the observation of hairline flaky texture, and the flaky texture of viewing head hair generally need adopt scanning electron microscope, but the scanning electron microscope specimen preparation is comparatively complicated, and equipment is comparatively expensive.The present invention adopts laser scanning co-focusing microscope viewing head hair, can collection head hair flaky texture image, and method is easy; Set up the method for visible light as light source analyzing hair silk flaky texture.
Summary of the invention
The purpose of this invention is to provide a kind of hairline formation method based on laser scanning co-focusing microscope, its technical scheme is: adopted 0.5% acridine orange mark hairline 15 minutes, and with deionized water rinsing 1 minute; Adopt the laser scanning hairline sample of laser scanning co-focusing microscope Argon ion laser 488nm wavelength, and adopt XYZ scanning sequence images acquired; The acquired signal wavelength is 520～600nm.This method is simple to operate, can observe the hairline flaky texture.
The present invention compared with prior art has following advantage:
1, easy and simple to handle, cost is low;
2, adopt visible light source to gather the fine structure of hair;
3, range of application is wider, can be used for the displaing microstructure observing of number of people hair or other animal hair.
Description of drawings
Accompanying drawing is the hairline flaky texture synoptic diagram of embodiments of the invention.
Embodiment
Embodiment 1
Clip hairline 1cm adopted 0.5% acridine orange mark hairline 15 minutes, and with deionized water rinsing 1 minute; Adopt the laser scanning hairline sample of laser scanning co-focusing microscope Argon ion laser 488nm wavelength, and adopt XYZ scanning sequence images acquired; The acquired signal wavelength is 520～600nm.This method is simple to operate, can observe the hairline flaky texture.
Claims (4)
1. the hairline formation method based on laser scanning co-focusing microscope is characterized in that: (1) employing 0.5% acridine orange mark hairline 15 minutes, usefulness deionized water rinsing 1 minute; (2) laser scanning, the images acquired of employing laser scanning co-focusing microscope Argon ion laser 488nm wavelength; The acquired signal wavelength is 520～600nm.(2) adopt laser scanning co-focusing microscope XYZ scanning sequence images acquired.This method is simple to operate, can observe the hairline flaky texture.
2. the hairline formation method based on laser scanning co-focusing microscope as claimed in claim 1 is characterized in that: adopt 0.5% acridine orange mark hairline, the mark time is 15 minutes.
3. the hairline formation method based on laser scanning co-focusing microscope as claimed in claim 1 is characterized in that: the laser scanning, the images acquired that adopt laser scanning co-focusing microscope Argon ion laser 488nm wavelength; The acquired signal wavelength is 520～600nm.
4. the hairline formation method based on laser scanning co-focusing microscope as claimed in claim 1 is characterized in that: adopt laser scanning co-focusing microscope XYZ scanning sequence images acquired.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010584822 CN102095705A (en) | 2010-12-09 | 2010-12-09 | Laser scanning confocal microscope-based hair imaging method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010584822 CN102095705A (en) | 2010-12-09 | 2010-12-09 | Laser scanning confocal microscope-based hair imaging method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102095705A true CN102095705A (en) | 2011-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201010584822 Pending CN102095705A (en) | 2010-12-09 | 2010-12-09 | Laser scanning confocal microscope-based hair imaging method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110780437A (en) * | 2019-05-10 | 2020-02-11 | 田允娴 | Repeated microscope imaging system for microscopic positions on human skin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1114359A (en) * | 1994-04-29 | 1996-01-03 | 临床诊断系统公司 | Homogenous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein |
| CN101522917A (en) * | 2006-08-11 | 2009-09-02 | 生物量子公司 | Method for assaying nucleic acids by fluorescence |
-
2010
- 2010-12-09 CN CN 201010584822 patent/CN102095705A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1114359A (en) * | 1994-04-29 | 1996-01-03 | 临床诊断系统公司 | Homogenous method for assay of double-stranded nucleic acids using fluorescent dyes and kit useful therein |
| CN101522917A (en) * | 2006-08-11 | 2009-09-02 | 生物量子公司 | Method for assaying nucleic acids by fluorescence |
Non-Patent Citations (3)
| Title |
|---|
| 《Advanced Biomedical and Clinical Diagnostic Systems VI,Proc. of SPIE Vol. 6848》 20081231 Chilhwan Oh1 et al Clinical applications of in vivo fluorescence confocal laser scanning microscopy 684814 1-4 , 2 * |
| 《COMPUTER AND COMPUTING TECHNOLOGIES IN AGRICULTURE II》 20091231 Zhiwei Chen et al CONFOCAL FLUORESCENCE MICROSCOPY OF MUNG BEAN LEAVES 1-4 第3卷, 2 * |
| 《J.Soc.Cosmet.Chem.》 19930228 PIERRE CORCUFF et al 3D Reconstruction of human hair by confocal microscopy 1-12 1-4 第44卷, 2 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110780437A (en) * | 2019-05-10 | 2020-02-11 | 田允娴 | Repeated microscope imaging system for microscopic positions on human skin |
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Application publication date: 20110615 |