CN102091348B - Porous beta-calcium phosphate biological scaffold material and preparation method thereof - Google Patents
Porous beta-calcium phosphate biological scaffold material and preparation method thereof Download PDFInfo
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- CN102091348B CN102091348B CN201110030831XA CN201110030831A CN102091348B CN 102091348 B CN102091348 B CN 102091348B CN 201110030831X A CN201110030831X A CN 201110030831XA CN 201110030831 A CN201110030831 A CN 201110030831A CN 102091348 B CN102091348 B CN 102091348B
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- calcium phosphate
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- biological scaffold
- porous
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Abstract
The invention relates to a porous beta-calcium phosphate biological scaffold material and a preparation method thereof. The invention adopts a technical scheme that: uniformly mixing CaCO3 and NH3H2PO4 according to a molar ratio of (1.3-1.8):1, performing machine molding under a pressure of 50 to 200MPa, and drying the molded blank body at 100 to 120 DEG C for 16 to 48 hours; and performing heat preservation for 1 to 8 hours at 900 to 1,200 DEG C to obtain the porous beta-calcium phosphate biological scaffold material. In the invention, the process is simple, the porous structure is controllable, the pressure strength of the product is 2 to 15MPa, the porosity is 35 to 75 percent and the aperture size of the pores is 20 to 150 mu m; therefore, more open pores are available, the pressure strength is high, and the purity of the beta-calcium phosphate phase is high. These pores are favorable for cells, fiber tissues, blood vessel tissues and other organic tissues to grow in, and the material is easy to degrade and can be used as repair materials for defected hard tissues of human bodies and used as a scaffold material for bone tissue engineering, and can also be used as a dental repair material.
Description
Technical field
The invention belongs to the biologic bracket material technical field.Be specifically related to a kind of porous beta-calcium phosphate biological scaffold material and preparation method thereof.Be mainly used in the timbering material in bone defect repair and organizational project, also can be used as the gear division repair materials.
Background technology
Biologic bracket material as the extracellular matrix of seed cell, the new organization framework that forms, is one of three key factors in organizational project, and its character directly affects the biological characteristicses such as the absorption, propagation, differentiation of seed cell, finally affects tissue construction.Desirable tissue engineering bracket material is answered form, structure, the function of simulated tissue, biocompatibility is preferably arranged, degradable, also should have certain intensity and loose structure, because suitable aperture, porosity and larger area-volume ratio, be conducive to cell adhesion, propagation, differentiation, for Growth of Cells provides sufficient space.Beta-calcium phosphate has biocompatibility preferably, is degradable biomaterial, and good biomechanical property is also arranged, and is suitable for doing the biologic bracket material in organizational project.
Traditional beta-calcium phosphate powder be prepared with wet method, dry method and hydro-thermal method.Wet method comprises two kinds: a kind of is acid, aqueous slkali direct reaction, namely at ambient temperature certain density phosphoric acid is added dropwise to Ca (OH)
2In suspension, staticly settle rear filtration, get the former powder of beta-calcium phosphate; Another kind is soluble calcium salt and phosphate reaction, is generally with certain density (NH under room temperature and stirring condition
4)
2HPO
4Aqueous solution is added drop-wise to Ca (NO by certain speed
3)
2In solution, through ageing, filtration washing, drying, be sintered into the beta-calcium phosphate powder.Hydro-thermal method is used less, is generally under hydrothermal condition, controls temperature and pressure, with CaHPO
4Or CaHPO
42H
2O is that raw material makes calcium phosphate powder; Dry method is under hot conditions (＞900 ℃), with CaHPO
42H
2O and CaCO
3Or Ca (OH)
2Solid state reaction occurs prepare the higher beta-calcium phosphate powder of purity.
The beta-calcium phosphate experiment condition of traditional wet method and hydro-thermal method preparation is complicated, and troublesome poeration, and the just powder that obtains need to be reconfigured, and loose structure should not be controlled, and intensity is not high yet; And to add some bonding agent and porogen during traditional dry process, and often there is dephasign to exist, the purity of beta-calcium phosphate image is not high.
Summary of the invention
The present invention is intended to overcome the prior art defective, and purpose is to provide the preparation method of the beta-calcium phosphate biologic bracket material of the porous that a kind of processing technology is simple, air hole structure is controlled; Porous beta-calcium phosphate biological scaffold material open pore with the method preparation is many, purity that have some strength and beta-calcium phosphate image is higher.
For achieving the above object, the technical solution used in the present invention is: first with CaCO
3And NH
4H
2PO
4Be in molar ratio (1.3～1.8): 1 mix homogeneously, be mechanical pressing under 50～200MPa condition at pressure again, base substrate after molding under 100～120 ℃ of conditions dry 16～48 hours, then under 900～1500 ℃ of conditions, be incubated 1～8 hour and burn till, namely get porous beta-calcium phosphate biological scaffold material.
In technique scheme: CaCO
3Particle diameter be 40nm～25 μ m, CaCO
3Purity＞99wt%; NH
4H
2PO
4Particle diameter be 88 μ m～200 μ m, NH
4H
2PO
4Purity＞99wt%.
Owing to adopting technique scheme, the simple gas of processing technology of the present invention, pore structure controlled (being that aperture size size and voidage can be controlled by granularity, briquetting pressure and the sintering temperature of raw material); Adopt the beta-calcium phosphate biologic bracket material open pore of porous of method of the present invention preparation many, have some strength, mainly phase is beta-calcium phosphate.After testing: the compressive resistance of this biologic bracket material is 2～15MPa, porosity is 35～75%, the air vent aperture size is between 20～150 μ m, these pores are conducive to growing into of cell, fibrous tissue, vascular tissue and other organic organization, be easy to degraded, can be used as the timbering material that the damaged repair materials of human body hard tissue and bone tissue engineer are used, also can be used as the gear division repair materials.
Therefore, preparation technology of the present invention is simple, and air hole structure is controlled; Prepared porous beta-calcium phosphate biological scaffold material open pore is many, compressive resistance is higher and the purity of beta-calcium phosphate image is higher.Can be used as the timbering material that the damaged repair materials of human body hard tissue and bone tissue engineer are used, also can be used as the gear division repair materials.
The specific embodiment
The present invention will be further described below in conjunction with the specific embodiment, is not the restriction to its protection domain.
In this specific embodiment: CaCO
3Purity＞99wt%; NH
4H
2PO
4Purity＞99wt%.Repeat no more in following examples.
Embodiment 1
A kind of porous beta-calcium phosphate biological scaffold material and preparation method thereof: be first the CaCO of 1 μ m～25 μ m with particle diameter
3NH with 200 μ m～160 μ m
4H
2PO
4Be in molar ratio (1.3～1.5): 1 mix homogeneously, be mechanical pressing under 50～100MPa condition at pressure again, base substrate after molding under 100～110 ℃ of conditions dry 16～24 hours, then under 900～1100 ℃ of conditions, be incubated 1～3 hour and burn till, namely get porous beta-calcium phosphate biological scaffold material.
Adopt said method, can obtain phase and be beta-calcium phosphate, aperture size and be 20～90 μ m, porosity and be 55～75% and burn after compressive resistance be the porous beta-calcium phosphate biological scaffold material of 2～5Mpa.
Embodiment 2
A kind of porous beta-calcium phosphate biological scaffold material and preparation method thereof: be first the CaCO of 500nm～1 μ m with particle diameter
3NH with 120 μ m～160 μ m
4H
2PO
4(1.5～1.6) in molar ratio: 1 mix homogeneously, be mechanical pressing under 100～150MPa condition at pressure again, base substrate after molding under 110～115 ℃ of conditions dry 24～36 hours, then under 1100～1300 ℃ of conditions, be incubated 3～5 hours and burn till, namely get porous beta-calcium phosphate biological scaffold material.
Adopt said method, can obtain phase and be beta-calcium phosphate, aperture size and be 30～120 μ m, porosity and be 45～70% and burn after compressive resistance be the porous beta-calcium phosphate biological scaffold material of 3～8Mpa.
Embodiment 3
A kind of porous beta-calcium phosphate biological scaffold material and preparation method thereof: be first the CaCO of 40nm～500nm with particle diameter
3NH with 88 μ m～120 μ m
4H
2PO
4Be in molar ratio (1.6～1.8): 1 mix homogeneously, mechanical pressing under the 150～200MPa condition of pressure position again, base substrate after molding under 115～120 ℃ of conditions dry 36～48 hours, then under 1300～1500 ℃ of conditions, be incubated 5～8 hours and burn till, namely get porous beta-calcium phosphate biological scaffold material.
Adopt said method, can obtain phase and be beta-calcium phosphate, aperture size and be 50～150 μ m, porosity and be 35～60% and burn after compressive resistance be the porous beta-calcium phosphate biological scaffold material of 5～15Mpa.
The technique of this specific embodiment is simple, air hole structure is controlled (being that aperture size size and voidage can be controlled by granularity, briquetting pressure and the sintering temperature of raw material); The beta-calcium phosphate biologic bracket material open pore of prepared porous is many, have some strength, main phase is beta-calcium phosphate.After testing: the compressive resistance of this biologic bracket material is 2～15MPa, porosity is 35～75%, the air vent aperture size is between 20～150 μ m, these pores are conducive to growing into of cell, fibrous tissue, vascular tissue and other organic organization, be easy to degraded, can be used as the timbering material that the damaged repair materials of human body hard tissue and bone tissue engineer are used, also can be used as the gear division repair materials.
Therefore, this specific embodiment technique is simple, and air hole structure is controlled; Prepared porous beta-calcium phosphate biological scaffold material open pore is many, compressive resistance is higher and the purity of beta-calcium phosphate image is higher.Can be used as the timbering material that the damaged repair materials of human body hard tissue and bone tissue engineer are used, also can be used as the gear division repair materials.
Claims (2)
1. the preparation method of a porous beta-calcium phosphate biological scaffold material, is characterized in that first with CaCO
3And NH
4H
2PO
4Be in molar ratio (1.3 ~ 1.8): 1 mix homogeneously, be mechanical pressing under 50 ~ 200MPa condition at pressure again, base substrate after molding under 100 ~ 120 ℃ of conditions dry 16 ~ 48 hours, then under 900 ~ 1500 ℃ of conditions, be incubated 1 ~ 8 hour and burn till, namely get porous beta-calcium phosphate biological scaffold material;
Described CaCO
3Particle diameter be 40nm ~ 25 μ m, CaCO
3Purity〉99wt%;
Described NH
4H
2PO
4Particle diameter be 88 μ m ~ 200 μ m, NH
4H
2PO
4Purity〉99wt%.
2. the prepared porous beta-calcium phosphate biological scaffold material of the preparation method of porous beta-calcium phosphate biological scaffold material according to claim 1.
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|---|---|---|---|
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|---|---|---|---|
| CN201110030831XA CN102091348B (en) | 2011-01-27 | 2011-01-27 | Porous beta-calcium phosphate biological scaffold material and preparation method thereof |
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|---|---|
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| CN102091348B true CN102091348B (en) | 2013-06-05 |
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|---|---|---|---|
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1488602A (en) * | 2003-08-12 | 2004-04-14 | 四川大学 | Porous calcium phosphate bioceramic material and preparing method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54158099A (en) * | 1977-05-25 | 1979-12-13 | Tokyo Ika Shika Daigakuchiyou | Preparation of artificial bone and artificial fang by means of apatite sintering body |
-
2011
- 2011-01-27 CN CN201110030831XA patent/CN102091348B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1488602A (en) * | 2003-08-12 | 2004-04-14 | 四川大学 | Porous calcium phosphate bioceramic material and preparing method thereof |
Non-Patent Citations (3)
| Title |
|---|
| JP昭54-158099A 1979.02.13 |
| 多孔β-TCP生物陶瓷制备工艺进展;陈志军 等;《生物骨材料与临床研究》;20080229;第5卷(第1期);49-52 * |
| 陈志军 等.多孔β-TCP生物陶瓷制备工艺进展.《生物骨材料与临床研究》.2008,第5卷(第1期),49-52. |
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