CN102077844A - Application of hydrogen peroxide in preparing Microcystin inhibitor - Google Patents
Application of hydrogen peroxide in preparing Microcystin inhibitor Download PDFInfo
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- CN102077844A CN102077844A CN2011100324863A CN201110032486A CN102077844A CN 102077844 A CN102077844 A CN 102077844A CN 2011100324863 A CN2011100324863 A CN 2011100324863A CN 201110032486 A CN201110032486 A CN 201110032486A CN 102077844 A CN102077844 A CN 102077844A
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- microcystin
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- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 title claims abstract description 42
- SRUWWOSWHXIIIA-UKPGNTDSSA-N Cyanoginosin Chemical compound N1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](C)[C@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C(=C)N(C)C(=O)CC[C@H](C(O)=O)N(C)C(=O)[C@@H](C)[C@@H]1\C=C\C(\C)=C\[C@H](C)[C@@H](O)CC1=CC=CC=C1 SRUWWOSWHXIIIA-UKPGNTDSSA-N 0.000 title claims abstract description 27
- 108010067094 microcystin Proteins 0.000 title claims abstract description 27
- 239000003112 inhibitor Substances 0.000 title claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000003643 water by type Substances 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 4
- 239000003053 toxin Substances 0.000 abstract description 13
- 231100000765 toxin Toxicity 0.000 abstract description 13
- 108700012359 toxins Proteins 0.000 abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 241000192710 Microcystis aeruginosa Species 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- 241000195493 Cryptophyta Species 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 239000013505 freshwater Substances 0.000 description 4
- 230000003834 intracellular effect Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000012851 eutrophication Methods 0.000 description 3
- 239000004009 herbicide Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 208000005374 Poisoning Diseases 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000002363 herbicidal effect Effects 0.000 description 2
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- 201000007270 liver cancer Diseases 0.000 description 2
- 210000005229 liver cell Anatomy 0.000 description 2
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- 230000003647 oxidation Effects 0.000 description 2
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- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 230000014639 sexual reproduction Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 229930195730 Aflatoxin Natural products 0.000 description 1
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010073069 Hepatic cancer Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
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- 239000005409 aflatoxin Substances 0.000 description 1
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- 238000009835 boiling Methods 0.000 description 1
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- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 206010073071 hepatocellular carcinoma Diseases 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 210000001865 kupffer cell Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- ZYZCGGRZINLQBL-GWRQVWKTSA-N microcystin-LR Chemical compound C([C@H](OC)[C@@H](C)\C=C(/C)\C=C\[C@H]1[C@@H](C(=O)N[C@H](CCC(=O)N(C)C(=C)C(=O)N[C@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]([C@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1)C(O)=O)C(O)=O)C)C1=CC=CC=C1 ZYZCGGRZINLQBL-GWRQVWKTSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- YZHUMGUJCQRKBT-UHFFFAOYSA-M sodium chlorate Chemical compound [Na+].[O-]Cl(=O)=O YZHUMGUJCQRKBT-UHFFFAOYSA-M 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
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- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
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Abstract
The invention provides application of hydrogen peroxide in preparing a Microcystin inhibitor. The experiment proves that hydrogen peroxide can inhibit synthesis and release of toxins in Microcystis aeruginosa cells, and prevent Microcystin from loss and from entering the water body system. In view of the advantages of high degradability of the Microcystin, the hydrogen peroxide can have wide application prospects in control of water bloom.
Description
(1) technical field
The present invention relates to the new application of hydrogen peroxide in preparation Microcystin inhibitor.
(2) background technology
The waters eutrophication has become global problem, and the eutrophication of freshwater lake causes the alga eruption sexual reproduction, forms wawter bloom.At present, Europe, Africa, North America and South America have 53%, 28%, 48%, 41% freshwater lake existence eutrophic state in various degree respectively, the lake of the Asian-Pacific area 54% is in eutrophic state, so that the main freshwater lake wawter bloom occurrence frequency of countries in the world and all constantly growths of the order of severity.The freshwater lake 75% above eutrophication of China is also often broken out large-scale wawter bloom phenomenon in recent years, as Chaohu blue-green algae in August, 2003 and in June, 2006 break out, the Taihu Lake in May, 2007 and the Dian Chi wawter bloom that happens occasionally.
The wawter bloom algae is poisoned by discharging secondary metabolites other biological generation of verifying, and wherein the most noticeable is Microcystin (Microcystin, MC).Microcystin is a kind of cell list ring seven peptide endotoxoid, mainly acts on liver cell and Kupffer cell, the activity of phosphoprotein phosphatase in the strong inhibition liver cell, and finally cause the generation of liver cancer.Epidemiology survey is the result also show, Microcystin content is closely related in primary carcinoma of liver and the drinking water source.Microcystin also has certain toxicity to animal, after domestic animal and wildlife have been drunk the water that contains Microcystin, symptoms such as diarrhoea, weak, apocleisis, vomiting can occur, even dead.There is the scholar to think that according to Hazard degree assessment Microcystin, hepatitis viruse, aflatoxin are China's south liver cancer 3 overall situation hazards occurred frequently.The problem that concerns of MC and human health has become one of focus of life science circle, medical circle and public's extensive concern.Over nearly 30 years, cause that by Microcystin the mankind and animal poisoning frequently take place all over the world.10000 people are arranged approximately owing to the water of drinking or directly contact the Microcystin pollution causes acute poisoning, cause that birds, fish, domestic animal poisoning also happen occasionally, cause animal deads such as cattle and sheep because of anabena, microcystis waterbloom every year as China Inner Mongol Da Laihu.Therefore, effectively control growth and the toxin secretion thereof of wawter bloom algae in the running water water source, become the problem that presses for solution.
The at present widely used algae medicine that removes has copper sulphate and weed killer herbicide etc., have and necessarily remove the algae effect, but copper sulphate and chemical herbicide can produce big negative effect to the non-target organism of aquatic ecological chain, and the generation secondary pollution needs careful using.
(3) summary of the invention
The invention provides the preparation that a kind of efficient, easy, free of contamination inhibition Microcystin is synthetic and discharge.
The technical solution used in the present invention is:
The application of hydrogen peroxide in preparation Microcystin (Microcystin) inhibitor.
Preferably, hydrogen peroxide can be used as inhibitor and is used for suppressing the synthetic of waters Microcystin and discharging, and especially suppresses the synthetic and release of microcystis aeruginosa toxin.
It is synthetic and when discharging that described hydrogen peroxide is used to suppress Microcystin, and throwing in concentration in the waters is 10～100 μ M.
A kind of active oxygen (ROS) that hydrogen peroxide produces in can the stimulation of biologic cells metabolic process because the ROS pair cell has the oxidation damaging action, thinks that always hydrogen peroxide is the reagent of the toxic effect of a kind of pair cell.Because hydrogen peroxide can oxidation Decomposition become the oxygen G﹠W under natural lighting catalysis, therefore the possibility that does not have secondary pollution, reasonably throw in the synthetic and release that hydrogen peroxide can suppress the algae toxin through experiment confirm, therefore can be used for preparing the Microcystin inhibitor, be used to administer the pollution of waterhead from the beginning that the alga eruption sexual reproduction causes.
The present invention confirms that by experiment hydrogen peroxide can suppress the synthetic and release of microcystic aeruginosa intracellular toxin, stops Microcystin to run off and enters the water body system, and in view of its advantage and readily degradable, hydrogen peroxide will have the applications well prospect on the control wawter bloom.
(4) description of drawings
Fig. 1 is the influence of hydrogen peroxide to microcystic aeruginosa intracellular toxin concentration;
Fig. 2 is hydrogen peroxide discharges toxin concentration to microcystic aeruginosa influence;
Fig. 3 produces total toxin concentration for hydrogen peroxide to microcystic aeruginosa.
(5) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
The cultivation of 1 microcystic aeruginosa
1) microcystic aeruginosa (Microcystis aeruginosa) is available from Wuhan aquatile research institute of the Chinese Academy of Sciences (numbering: 905), use the BG-11 medium culture.
2) condition of culture: 24 ± 0.5 ℃, Light To Dark Ratio is 14: 10, intensity of illumination: 4000Lux.
3) dosing: algae liquid is cultured to OD=0.05, adds hydrogen peroxidase 10,25,50 μ M respectively, is cultured to the 96h sampling.
2 Microcystin Determination on content
1) get 30ml algae liquid, the centrifugal supernatant that goes, boiling water boils 8min; Add 75% methyl alcohol, 200 μ l mixings, the centrifugal 20min of 14000rpm; Get supernatant and be used for intracellular microcyst algae content of toxins mensuration.
2) get 30ml algae liquid, the centrifuging and taking supernatant, evaporation and concentration is to 10ml; The centrifugal 20min of 14000rpm; Get supernatant and be used for extracellular Microcystin assay.
3) use HPLC to detect the microcapsule algae toxin standard sample, liquid-phase condition is:
Mobile phase methanol: water (containing 0.1% trifluoroacetic acid) volume ratio=7: 3
Chromatogram column temperature: 40 ℃;
Flow velocity: 1 μ l/min;
Sample size: 20 μ l;
Detector: ultraviolet-visible detector wavelength 238nm and drawing standard curve.4) same liquid-phase condition detects intracellular toxin and extracellular toxin content, and calculates toxin concentration with calibration curve gained formula, the results are shown in Figure 1～3.As seen from the figure, behind the hydrogen peroxide treatment microcystic aeruginosa 96h, born of the same parents' endotoxin content reduces, and the toxin total amount in the culture environment also significantly reduces.
Claims (3)
1. the application of hydrogen peroxide in preparation Microcystin (Microcystin) inhibitor.
2. application as claimed in claim 1 is characterized in that hydrogen peroxide is used for suppressing the synthetic of waters Microcystin and discharging as inhibitor.
3. application as claimed in claim 2 is characterized in that described inhibitor is thrown in concentration in the waters be 10～100 μ M.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110032486 CN102077844B (en) | 2011-01-30 | 2011-01-30 | Application of hydrogen peroxide in preparing Microcystin inhibitor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110032486 CN102077844B (en) | 2011-01-30 | 2011-01-30 | Application of hydrogen peroxide in preparing Microcystin inhibitor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102077844A true CN102077844A (en) | 2011-06-01 |
| CN102077844B CN102077844B (en) | 2013-06-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201110032486 Expired - Fee Related CN102077844B (en) | 2011-01-30 | 2011-01-30 | Application of hydrogen peroxide in preparing Microcystin inhibitor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107279491A (en) * | 2017-08-09 | 2017-10-24 | 江苏纳克生物工程有限公司 | A kind of method of Microcystis aeruginosa fodder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1594135A (en) * | 2004-07-02 | 2005-03-16 | 王培军 | Method for eliminating algae hazard in water body |
| CN1629083A (en) * | 2003-12-16 | 2005-06-22 | 中国科学院生态环境研究中心 | Electro-Fenton method and apparatus for removing multi-algae toxins from water |
-
2011
- 2011-01-30 CN CN 201110032486 patent/CN102077844B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1629083A (en) * | 2003-12-16 | 2005-06-22 | 中国科学院生态环境研究中心 | Electro-Fenton method and apparatus for removing multi-algae toxins from water |
| CN1594135A (en) * | 2004-07-02 | 2005-03-16 | 王培军 | Method for eliminating algae hazard in water body |
Non-Patent Citations (1)
| Title |
|---|
| 李效宇: "《微囊藻毒素及其毒理学研究》", 30 April 2007, article "微囊藻毒素的去除方法", pages: 78 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107279491A (en) * | 2017-08-09 | 2017-10-24 | 江苏纳克生物工程有限公司 | A kind of method of Microcystis aeruginosa fodder |
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| Publication number | Publication date |
|---|---|
| CN102077844B (en) | 2013-06-19 |
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Granted publication date: 20130619 |