CN102071158A - Low temperature resistant methane bacterium and preparation method thereof - Google Patents
Low temperature resistant methane bacterium and preparation method thereof Download PDFInfo
- Publication number
- CN102071158A CN102071158A CN2009102235744A CN200910223574A CN102071158A CN 102071158 A CN102071158 A CN 102071158A CN 2009102235744 A CN2009102235744 A CN 2009102235744A CN 200910223574 A CN200910223574 A CN 200910223574A CN 102071158 A CN102071158 A CN 102071158A
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- bacteria
- low temperature
- methane
- temperature resistant
- acid bacteria
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a low temperature resistant methane bacterium and a preparation method thereof and the methane bacterium effectively solves the defect that the gas output of the methane tank is low in winter. The preparation method of the low temperature resistant methane bacterium comprises the following steps: sampling and breeding five strains, namely acetic acid bacteria, lactic acid bacteria, butylic acid bacteria, saccharomycetes and methane bacteria from a northern methane tank in winter, performing low temperature breeding, performing pure solid culture and mixing in a ratio according to the number of microbial floras, which is suitable for methane production. The bacterium is bred and the preparation method is simple and cheap; by using the bacterium, the winter utilization rate of the northern methane tank can be effectively increased, the popularity of methane in rural areas can be extremely increased, the quality of life of rural population can be improved and the rural environment can be improved.
Description
Technical field
The invention belongs to the microbial fermentation technology field, specifically a kind of preparation method of compound microorganism ferments microbial inoculum.
Background technology
Should mostly adopt spontaneous fermentation or methods such as inoculation natural pond liquid, natural pond slag in the microorganism of biogas production at present.This kind method is influenced by environmental temperature, and especially northern area winter, it is little that gas production rate appears in methane-generating pit, or phenomenon such as aerogenesis not, causes the methane-generating pit cisco unity malfunction.The measure that major part is taked is incubated for methane-generating pit being taked thickening, or takes the solar energy impinges heating, temperature keeping to warm up measures such as circle, and its shortcoming is the initial cost that increases the user, to the transformation of old natural pond water, factors such as capital construction difficulty and input and output material inconvenience also occur.
Summary of the invention
The object of the present invention is to provide and a kind ofly form, and growth in low temperature environment, breeding, fermentation, production capacity produce the microbiobacterial agent of biogas in low temperature environment by multiple microorganism.
Technical scheme of the present invention is:
One, the present invention is by following bacterial classification and percentage counterweight ratio thereof, acetic bacteria 20-30%, milk-acid bacteria 20-30%, butyric bacteria 10-20%, yeast 5-10%, methanobacteria 20-30%.
Two, preparation process of the present invention:
1. be lower than 0 ℃ area at the northern area winter temperature, selection can produce the methane-generating pit of biogas more than 10, and 50-100g takes a sample respectively.
2. above-mentioned 10 samples are carried out isolation identification in the laboratory, adopt selective medium, select acetic acid flora, butyric acid flora, lactobacillus, yeast flora, methane flora respectively.
3. above-mentioned 5 strainses are carried out single culture, culture condition is:
Substratum is 1～2% multitudinous sugar, 5-10% soybean cake powder, 3-4% peptone, 0.5-1% extractum carnis, 0.5-1%mgSO
47H
2O, 0.03-0.07% (NH
4)
2SO
4, 0.01-0.03KH
2PO
4And 0.01-0.05%Na
2HPO
4Incubation time 2-4 days.Culture temperature is done once since 2 ℃ of 15 ℃ of every declines and is cultivated, and directly causes bacterium number propagation less than till the 20-100%.Inoculum size is 1-5%, and inoculum is preceding temperature degree of passing a decline bacterium liquid.
4. select 5 ℃ of yeast minimum temperatures thus, 3 ℃ of milk-acid bacteria minimum temperatures, 4 ℃ of acetic bacteria temperature, 8 ℃ of butyric bacteria temperature, 7 ℃ of methanobacteria temperature.
This psychrophile is inserted slant tube refrigeration, insert solid medium simultaneously, cultivate after 3 days, obtain every gram microbial inoculum acetic bacteria, milk-acid bacteria, butyric bacteria, yeast, methanobacteria, containing the bacterium number is 0.5-1.2 * 10
8, 0.3-0.6 * 10
8, 0.2-0.4 * 10
8, 0.3-0.4 * 10
8, 1.2-1.6 * 10
8Individual.
5. above-mentioned five energy are carried out bacterial classification refrigeration at the bacterial classification inoculation of low temperature environment growth and breeding in slant tube.
6. will select five kinds of bacterial classifications and be seeded in respectively in the above-mentioned substratum and cultivate, and make in every gram solid medium acetic bacteria, milk-acid bacteria, butyric bacteria, methanobacteria contain the bacterium number and reach 0.5-1.2 * 10
8, 0.3-0.6 * 10
8, 0.2-0.4 * 10
8, 0.3-0.4 * 10
8, 1.2-1.6 * 10
8Individual.
7. by each bacterial classification percentage counterweight ratio, acetic bacteria 20-30%, milk-acid bacteria 20-30%, butyric bacteria 10-20%, yeast 5-10%, methanobacteria 20-30% carry out compound, and the various bacterium numbers in compound back are 1 * 10
7More than, effectively control bacterium in every gram composite fungus agent and count 1-10 * 10
8Individual, water content≤10%.
Embodiment
Choose 10 samples in regional winter such as the Inner Mongol, Hebei, Shanxi, Yinchuan, in acetic bacteria, butyric bacteria, milk-acid bacteria, yeast, methanobacteria substratum, cultivate respectively, observe in microscopy and the substratum and select bacterial strain separately.Bacterial strain inserts 1.5% multitudinous sugar, 6% soybean cake powder, 3.2% peptone, 0.8% extractum carnis, 0.5%mgSO separately
47H
2O, 0.04% (NH
4)
2SO
4, 0.02KH
2PO
4And 0.02%Na
2HPO
4In the substratum.Inoculum size 5%PH6.5-7.0, culture temperature is respectively degree centigrade 15 ℃, 13 ℃, 11 ℃, 9 ℃, 8 ℃, 7 ℃, 6 ℃, 5 ℃, 4 ℃ cultivations.
Stop inoculation culture by propagation less than 30%, reach 5 ℃ of yeast minimum temperatures, 3 ℃ of milk-acid bacteria minimum temperatures, 4 ℃ of acetic bacteria temperature, 8 ℃ of butyric bacteria temperature, 7 ℃ of methanobacteria temperature.
This psychrophile is inserted slant tube refrigeration, insert solid medium simultaneously, cultivate after 3 days, obtain every gram microbial inoculum acetic bacteria, milk-acid bacteria, butyric bacteria, yeast, methanobacteria, containing the bacterium number is 0.5-1.2 * 10
8, 0.3-0.6 * 10
8, 0.2-0.4 * 10
8, 0.3-0.4 * 10
8, 1.2-1.6 * 10
8Individual.
This microbial inoculum on average improves pond temperature 3-5 ℃ methane-generating pit test 8 times, and the bacterium number increases by 60%, and producing firedamp gas increases by 60%, and effect is remarkable.
Claims (6)
1. low temperature resistant biogas microbial inoculum and preparation method thereof is characterized in that under the microthermal climate condition, the methane-generating pit microorganism species is carried out seed selection is composite to form.
2. low temperature resistant biogas microbial inoculum according to claim 1 is characterized in that, is to isolate acetic bacteria, milk-acid bacteria, butyric bacteria, yeast, methanobacteria in low temperature environment from natural pond liquid, natural pond slag.
3. low temperature resistant biogas microbial inoculum according to claim 1 is characterized in that acetic bacteria, milk-acid bacteria, butyric bacteria, yeast, methanobacteria are lower than seed selection below 15 ℃ in culture temperature.
4. low temperature resistant biogas microbial inoculum according to claim 1, it is characterized in that the substratum of seed selection acetic bacteria, milk-acid bacteria, butyric bacteria, yeast, methanobacteria is 1～2% multitudinous sugar, 5-10% soybean cake powder, 3-4% peptone, 0.5-1% extractum carnis, 0.5-1%mgSO
47H
2O, 0.03-0.07% (NH
4)
2SO
4, 0.01-0.03KH
2PO
4And 0.01-0.05%Na
2HPO
4
5. low temperature resistant biogas microbial inoculum according to claim 1 is characterized in that, by following bacterial classification and percentage counterweight thereof than forming acetic bacteria 20-30%, milk-acid bacteria 20-30%, butyric bacteria 10-20%, yeast 5-10%, methanobacteria 20-30%.
6. low temperature resistant biogas microbial inoculum according to claim 1 is characterized in that, in every gram solid medium, acetic bacteria, milk-acid bacteria, butyric bacteria, yeast, methanobacteria contain the bacterium number and reach 0.5-1.2 * 10
8, 0.3-0.6 * 10
8, 0.2-0.4 * 10
8, 0.3-0.4 * 10
8, 1.2-1.6 * 10
8Individual.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102235744A CN102071158A (en) | 2009-11-24 | 2009-11-24 | Low temperature resistant methane bacterium and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102235744A CN102071158A (en) | 2009-11-24 | 2009-11-24 | Low temperature resistant methane bacterium and preparation method thereof |
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|---|---|
| CN102071158A true CN102071158A (en) | 2011-05-25 |
Family
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| CN2009102235744A Pending CN102071158A (en) | 2009-11-24 | 2009-11-24 | Low temperature resistant methane bacterium and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876618A (en) * | 2012-10-22 | 2013-01-16 | 新疆农业科学院微生物应用研究所 | Solid composite accelerant for low-temperature methane and preparation method thereof |
| CN103266061A (en) * | 2013-06-14 | 2013-08-28 | 马凯 | Low-temperature biogas leavening agent and preparation method thereof |
| CN111909967A (en) * | 2019-05-07 | 2020-11-10 | 湖南省土壤肥料研究所 | Method for increasing content and volume of methane gas |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101818173A (en) * | 2010-03-23 | 2010-09-01 | 河南农业大学 | Process for producing biogas from crop straw by dry fermentation |
-
2009
- 2009-11-24 CN CN2009102235744A patent/CN102071158A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101818173A (en) * | 2010-03-23 | 2010-09-01 | 河南农业大学 | Process for producing biogas from crop straw by dry fermentation |
Non-Patent Citations (3)
| Title |
|---|
| 张录 等: "沼气两步法发酵工艺微生物生态学的研究", 《中国沼气》 * |
| 杨鹏可 等: "偏低温沼气发酵促进剂的初步研究", 《酿酒科技》 * |
| 邓功成 等: "沼气发酵微生物低温驯化研究", 《安徽农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876618A (en) * | 2012-10-22 | 2013-01-16 | 新疆农业科学院微生物应用研究所 | Solid composite accelerant for low-temperature methane and preparation method thereof |
| CN102876618B (en) * | 2012-10-22 | 2014-12-17 | 新疆农业科学院微生物应用研究所 | Solid composite accelerant for low-temperature methane and preparation method thereof |
| CN103266061A (en) * | 2013-06-14 | 2013-08-28 | 马凯 | Low-temperature biogas leavening agent and preparation method thereof |
| CN111909967A (en) * | 2019-05-07 | 2020-11-10 | 湖南省土壤肥料研究所 | Method for increasing content and volume of methane gas |
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Application publication date: 20110525 |