CN102047827B - Method for promoting content of grape resveratrol - Google Patents
Method for promoting content of grape resveratrol Download PDFInfo
- Publication number
- CN102047827B CN102047827B CN2010105090468A CN201010509046A CN102047827B CN 102047827 B CN102047827 B CN 102047827B CN 2010105090468 A CN2010105090468 A CN 2010105090468A CN 201010509046 A CN201010509046 A CN 201010509046A CN 102047827 B CN102047827 B CN 102047827B
- Authority
- CN
- China
- Prior art keywords
- grape
- water
- derivant
- parts
- resveratrol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention provides a method for promoting content of resveratrol in grape leaves, grape stems and grape fruits, which comprises preparation and dilution of colloid aluminum inductive agent and dry powder, and the relative use method thereof in the induction of the grape materials. The method of the invention has the characteristics of high efficiency, stability, low residue and low toxicity in promotion of the resveratrol in induced grape fruit and the relative by-products (such as leaves and stems) in the grape production. The method of the invention does not need precise instrument or automatic equipment, can be applied in a large scale in town and township enterprises with rich grape resources, not only can increase the grape quality, but also can improve the comprehensive utilization of the grape planting wastes, and can lead the grape trees to generate a certain resistance to pathogenic bacteria, thus decreasing the loss caused by plant diseases.
Description
Technical field
The present invention relates to the method for Resveratrol content in a kind of grape leaf that raises, grape stem and the grape fruit.
Background technology
(resveratrol:trans-3,5,4 '-trihydroxystilbene) is a kind of stilbene compound (stilbenes) to resveratrol.Siemann in 1992 and Creasy at first confirm to contain in the wine natural trans-resveratrol, and for people's liver, skin, heart and blood circulation, lipid metabolism important conditioning drug effect are arranged.Found also in the last few years that resveratrol had special effect at anti-inflammatory, cardiovascular protection, oestrogenic hormone adjusting and anti-tumor aspect, its medical value gets more and more people's extensive concerning.
Resveratrol is present in some medicinal plants and the food plant, like Cassia tora, black false hellebore, giant knotweed, grape, peanut.Aspect the resveratrol acquiring way, select suitable vegetable material to become this field hot issue.The source of present commercially available resveratrol mainly is from polygonaceae plant giant knotweed rhizome, to extract.For example publication number is that the Chinese patent of CN1321961C, CN101698634A, CN1566349A, CN1341587A, CN101709024A has adopted the method for from giant knotweed, extracting resveratrol.Giant knotweed is a kind of than precious Chinese herbal medicine, because its resource is suffering comparatively serious destruction, existing market is on the high side.There is large-area wine-growing in China, and this makes that utilizing grape resource to obtain resveratrol becomes possibility.For example publication number has adopted the method for from the grape pip material, extracting resveratrol for the CN101704729A Chinese patent; Publication number is that the Chinese patent of CN1351985 has adopted the method for from the Grape Skin material, extracting resveratrol; Publication number adopts the method for from grape stem material, extracting resveratrol for the US20090191294A1 United States Patent (USP).People such as Li Ting 2009 are at " comparison of grape different tissues position Resveratrol content " (Gansu Agriculture University,'s journal; 2009; 44:64-67) measured the content of resveratrol in the grape different tissues in the literary composition, found that grape different tissues position Resveratrol content order from high to low is carpopodium, blade＞pericarp＞seed＞petiole.People such as Park HJ 2009 are at " Contents of trans-resveratrol in Korean grape cultivars; including " Kyoho " " (J.Plant Biol.; 2009; 52:319-324) measured position Resveratrol contents such as blade, seed, pericarp in the literary composition, found that the blade Resveratrol content is the highest.This explanation grape leave also can be used as resveratrol and extracts raw material.
Although the existence of resveratrol is all found at each position of grape.Often because the contained resveratrol level of materials such as grape leave, fruit changes greatly, be difficult to keep stable during practical operation, influence these materials extract raw material as resveratrol precedence.Under normal circumstances, the Resveratrol content of newborn grape leaf remains on reduced levels." Role of stilbene inresistance of wood to decay " (Phytopathology that Hart JH delivered in 1979,1981; 1979; 69:1138-1143) with " Role of phytostilbenes in decay and disease resistance " (phytopathology; 1981; 19:437-458) people such as two pieces of articles and Sbaghi delivers " Development of methods using phytoalexin (resveratrol) assessmentas a selection criterion to screen grapevine in vitro cultures forresistance to grey mould (Botrytis cinerea) " (Duphytica nineteen ninety-five; 1995,86:41-47) all propose in the literary composition to receive biological factor such as naturally-aged, pathogen infection, or environmental factor such as high temperature, ultraviolet irradiation, ozone and carbonic acid gas etc. are when influencing when grape; Resveratrol content has obvious increase, and unstable.Because these biologies and environmental factor are uncontrollable; Utilize these methods to induce the method for grape resveratrol accumulation to realize comparatively difficulty in the field; Be difficult to ensure through induced material Resveratrol content stable before extraction, even can have influence on the output and the quality of grape.Therefore select raise Resveratrol content in grape leave and other tissue of stable derivant to become the focus of research.
In the operation of inducing the grape resveratrol to raise through various chemical inducers, induced material and processing mode be selected to the key of inducing.For example, France and Jeandet Adrian, who in 1996 published "Induction? Of? Phytoalexin (resveratrol) synthesis? In? Grapevine? Leavestreated? With? Aluminum? Chloride" (J.Agric.Food? Chem., 1996,44:1979 - 1981) in an article with 7-month-old grape stems, leaf material from the top 4-15, wherein the in vitro cuttings of grape leaves into the aqueous solution of aluminum chloride for induction treatment, the operation of the sample, there are strict requirements, time-consuming to operate laborious and can not achieve large-scale induction; U.S. Patent Publication No. US006080701A aluminum chloride is used as the main ingredient is passed through inducing agent in vivo treatment of the grape leaves elevated contents of resveratrol, characterized in that the use of stem injections, sprays, and the field leaf painting and other ways to induce its operational aspects of stem injection does not take to the field labor costs, spraying or painting operation is not considered grapes absorb residual aluminum and other issues; Qi Guimei and Greasy in 2005 published an article "fosetyl and ultraviolet Grape leaves irradiation on stilbene compounds induced "(foreign grapes and wine ,2005,4:12-16), wherein the use of special parts of grapes and in vitro in vivo inducer blades and light irradiation induced smear treatment , the induction sessions and made the operation involves many factors, it is difficult to apply in the field production.
In the operation of inducing grape leave accumulation resveratrol, selecting suitable derivant is to stablize the key of inducing.People such as France Adrian and Jeandet delivered " Induction ofphytoalexin (resveratrol) synthesis in grapevine leaves treated withAluminum chloride " (J.Agric.Food Chem. in 1996; 1996,44:1979-1981) adopt acid chlorization aluminium ex vivo treatment mode to make Resveratrol content rising in the grape leaf in the literary composition.Wherein the concentration of acidic select 7-90mM aqueous solution of aluminum chloride soaked, handling minimum time of 9 hours, resveratrol induced group and leaf fresh weight ratio of up to 300-400μg / g, when the aluminum concentration is greater than 90mM Leaves serious toxic and cause resveratrol decreased; U.S. Patent Publication No. US006080701A aluminum chloride is used as the main ingredient is passed through inducing agent in vivo treatment of the grape leaves elevated contents of resveratrol, characterized in that the treatment agent mass percentage of aluminum chloride concentration of 0.3-4%, from 0.1 to 100 kg per hectare inducer resveratrol induced group and leaf fresh weight ratio of up to 426.63μg / g; Qi Guimei published in 2005 and Greasy "Fosetyl and ultraviolet radiation on grape leaves stilbene compounds induced" (foreign grapes and wine ,2005,4:12-16) an article, wherein the use of soluble Fosetyl and UV irradiation on leaf samples for induction treatment, fosetyl aluminum salt solution is sprayed from leaf concentrations ranging 0-40g / L and irradiated with UV for 10 minutes after induction in leaves resveratrol from 1.4μg / g was increased to 17μg / g, of grape Leaves for induction in vitro, no significant effect; these inducers used during operation may cause some of the operating personnel poison; these documents and the methods used are proprietary resveratrol increased the target leaves, but leaves does not exceed the maximum amount of resveratrol-induced 450μg / g; these inducers not take into account changes in grape berry resveratrol.When especially handling implementing to induce at the body grape leave through soluble aluminum, the stripping of aluminium may cause the grape underproduction even can cause aluminium residual in grape fruit; The phytotoxicity of acidic aluminum is become final conclusion in agricultural production; For example people such as Foy CD was in (Annu.Rev.Plant Physiol. in 1978; 29:511-566) deliver people such as " The physiology of metal toxicity in plant " literary composition and inventor Zhang Bo in " Aluminum uptake and disease resistance in Nicotianarustica leaves " (the Ecotoxicology and environmental safety that delivers in 2010; 2010; Even if 73:655-663) find that all micro-soluble aluminum (20 μ M) also can produce tangible toxic action like inhibition growth, damaged blade to plant, and show the dose-dependent effect of damage along with the increase of aluminium stripping concentration.So the practice of above-mentioned Resveratrol Inducing agent employing soluble aluminum such as acid chlorization aluminium, aliette is difficult to carry out the land for growing field crops and promotes.
China's wine-growing area was nearly 6,000,000 mu in 2002.Along with the increase wine-growing area to grape Related product demand presents fast rise trend, 2009 only Xinjiang Uygur Autonomous Regions's wine-growing area just up to 1,500,000 mu.In order to make full use of this advantage and resource; To the existing deficiency that improves grape Resveratrol content method; Realistic meaning of the present invention is to provide a kind of increase grape quality, improves grape healthy nutritive value and grape production related by-products comprehensive utilization method, can alleviate the contradiction of resveratrol increase in demand and raw materials for production giant knotweed resource growing tension again.
Summary of the invention
The object of the present invention is to provide the method that resveratrol raises in grape fruit and the grape production related by-products (blade, stem) of inducing of a kind of efficient, stable, low-residual, low toxicity.
The present invention seeks to realize through following technical scheme:
The preparation of a, derivant: the NaOH of 40 weight portions is dissolved in the water of 1000 weight portions and makes sodium hydroxide solution; With the anhydrous Aluminum chloride of 50-100 weight portion or the AlCl of 90-250 weight portion
36H
2O is dissolved in the water of 1000 weight portions and makes liquor alumini chloridi; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially drip speed and be not less than 100ml/ minute, mixing speed 800-2000 rev/min; 60-70 ℃ of water-bath heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 50-100ml/ minute; Survey the pH value between 5.5-6.8; Stop to stir, left standstill 24 hours, it is subsequent use to treat to get after colloid-stabilised colloidal aluminum;
The dilution of b, derivant: select the water of the 0-100 times of weight portion of colloidal aluminum adding of 1 weight portion, mixing speed 800-2000 rev/min, colloid-stabilised then the use as liquid derivant treated in 60-70 ℃ of water-bath heating;
C, the preparation of dry powder derivant: the colloidal aluminum that step a makes is carried out drying, behind the removal moisture, cross 100 eye mesh screens, get the dry powder derivant;
D, induce processing: the liquid derivant that above-mentioned steps b is made sprays vine, the vine acrial part is sprayed the liquid derivant of 1-10 weight portion with per hectare biomass 500 weight portions induce processing; The liquid derivant 1-10 weight portion that above-mentioned steps b is made is sprayed onto the 20-200 weight portion and exsomatizes and induce processing on grape leaf, stem and the fruit; The dry powder derivant that above-mentioned steps c is made sprays vine, the vine acrial part is sprayed 0.1-10 weight portion dry powder derivant with 5000 parts of per hectare biomasss induce processing; The dry powder derivant 0.1-10 weight portion that above-mentioned steps c is made is sprayed onto the 200-2000 weight portion and exsomatizes and induce processing on grape leaf, stem and the fruit;
Water described in above-mentioned steps a, the b can be any or mixing of running water, distilled water, the deionized water of pH＜7.
Drying described in the above-mentioned steps c is atomized drying or bake drying, and the dry run temperature is less than 100 ℃.
The processing time of inducing described in the above-mentioned steps d is not less than 10 hours.
Beneficial effect of the present invention:
The method of selecting stablize rising grape leave and other position Resveratrol content is to use material separation such as the alternative giant knotweed of grape associated materials to prepare the key of resveratrol; Beneficial effect of the present invention is the Resveratrol content rising in body and exsomatize grape leave, grape stem and fruit of inducing that institute's method for using can be stable; Induce effect can owing to resveratrol extract before inevitably illumination; Insect pest; Pluck, biology such as temperature and environmental factor change and take place and obviously descend, and have ensured that the grape material induces the stability of Resveratrol content;
Selecting the method for operating of inducing of simple and convenient is the key of field size rising grape Resveratrol content operation; Beneficial effect of the present invention be the abductive approach that uses simple to operate; Only relate to a kind of inducer; Only relate to and spray an operation link; No matter be that liquid derivant or dry powder derivant all are convenient to carry out spraying operation, without wash-out, derivant operation sprinkling process can not cause the person to poison to operating personnel after the sprinkling; Beneficial effect of the present invention also is the grape fruit to any one stage in the wine-growing process; Blade and stem all can be induced operation; Method therefor does not limit raw-material vegetative stage, the guarantee of maximum likelihood the versatility and the ease for use of material;
Selecting suitable derivant is the key of carrying out the grape Resveratrol Inducing; The present invention selects aluminium and the dried powder of colloidal aluminum of colloidal attitude as derivant; Derivant is high more in the past owing to pH in these aluminium agent; Dissociate and have slowly releasing effect in that plant surface is atomic, thus can be in operating process a large amount of strippings and the plant poisoning effect of acidic aluminum takes place; Because the chemical property of soil, the used derivant of the present invention water clock and being scattered in the sprinkling process all can not impact field soil; Because the indissoluble characteristic of the institute colloidal aluminum of using and dry pulvis thereof, when inducing the grape resveratrol to raise, extremely difficult by grape absorb, transhipment, accumulation, so can absorption be not residual in a large number in grape fruit inside, the health that can not endanger the eater;
Selecting the derivant optimum amount is the key of carrying out the grape Resveratrol Inducing; Beneficial effect of the present invention is that the consumption of colloidal aluminum and dry powder thereof of selected use when ensureing stable inducing, has higher inductivity; Method used herein induces the Resveratrol content maximum to be elevated to 30 times in the blade level, and Resveratrol content is up to 883 μ g/g with the leaf fresh weight ratio; Method used herein also has significantly inducing action to excised leaf, and excised leaf Resveratrol content maximum is elevated to 3 times; Method used herein is induced at body fruit Resveratrol content and on average is elevated to 2-5 doubly, induces the fruit Resveratrol content maximum that exsomatizes to be elevated to 2 times; Method used herein also has more stable inducing action to stem, induces the stem Resveratrol content that exsomatizes to be elevated to 3-5 doubly;
The inventive method does not need precision instrument or automation equipment; Can carry out large-scale application in the abundant township enterprise of grape resource; Both can increase grape quality also can increase the comprehensive utilization of wine-growing discarded object; Belong to typical protective plant protecting agent by resveratrol, its content raises in implementation process and can make vine that pathogen is produced certain resistance, reduces the caused loss of disease;
Embodiment
Below, the present invention will further explain with embodiment, but it is not limited to any one or the similar instance of these embodiment.
Embodiment 1
40 parts NaOH are dissolved in 1000 parts the water; 80 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1200 rev/mins of mixing speeds fast 200ml/ minute; 65 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 75ml/ minute; Drop to pH value of solution value 6.8; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 50 times of weight portions of colloidal aluminum adding of 1 part, 1200 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 60 ℃ of water-baths heating;
The liquid derivant that makes is sprayed field planting shoot growth phase American red globe grape tree, the vine acrial part is sprayed 10 parts of liquid derivants with 500 parts of per hectare biomasss induce processing; 10 hours processing times; HPLC records the blade Resveratrol content and is elevated to 10 times, and the average Resveratrol content fresh weight ratio of blade reaches 242 μ g/g, and the fruit Resveratrol content is elevated to 4 times, is tried the little downy mildew resistance bacterium of strain.
Embodiment 2
40 parts NaOH are dissolved in 1000 parts the water; 60 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 800 rev/mins of mixing speeds fast 120ml/ minute; 60 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 50ml/ minute; Drop to pH value of solution value 6.5; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 20 times of weight portions of colloidal aluminum adding of 1 part, 900 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 70 ℃ of water-baths heating;
The liquid derivant that makes is vowed that to field planting the Fu Luosha vine sprays flowering stage, the vine acrial part is sprayed 8 parts of liquid derivants with 500 parts of per hectare biomasss induce processing; 15 hours processing times; HPLC records the blade Resveratrol content and is elevated to 8 times, and the average Resveratrol content fresh weight ratio of blade reaches 335 μ g/g, and the fruit Resveratrol content is elevated to 5 times, is tried frost-resistant mould in the strain.
Embodiment 3
40 parts NaOH are dissolved in 1000 parts the water; 50 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1500 rev/mins of mixing speeds fast 250ml/ minute; 70 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 100ml/ minute; Drop to pH value of solution value 6.0; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 10 times of weight portions of colloidal aluminum adding of 1 part, 800 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 65 ℃ of water-baths heating;
With the liquid derivant that makes to the field planting Berry Growth phase seedless purple grape tree spray, the vine acrial part is sprayed 5 parts of liquid derivants with 500 parts of per hectare biomasss induces processing; 18 hours processing times; HPLC records the blade Resveratrol content and is elevated to 30 times, and the average Resveratrol content fresh weight ratio of blade reaches 883 μ g/g, and the fruit Resveratrol content is elevated to 5 times, is tried anti-white rot germ in the strain.
Embodiment 4
40 parts NaOH are dissolved in 1000 parts the water; 90 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 2000 rev/mins of mixing speeds fast 250ml/ minute; 70 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 100ml/ minute; Drop to pH value of solution value 5.5; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 5 times of weight portions of colloidal aluminum adding of 1 part, 1500 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 68 ℃ of water-baths heating;
The liquid derivant that makes is sprayed field planting berry maturing stage cabernet sauvignon grape tree, the vine acrial part is sprayed 2 parts of liquid derivants with 500 parts of per hectare biomasss induce processing; 20 hours processing times; HPLC records the blade Resveratrol content and is elevated to 5 times, and the average Resveratrol content fresh weight ratio of blade reaches 174 μ g/g, and the fruit Resveratrol content is elevated to 4 times.
Embodiment 5
40 parts NaOH are dissolved in 1000 parts the water; AlCl with 90 parts
36H
2O is dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1000 rev/mins of mixing speeds fast 140ml/ minute; 65 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 55ml/ minute; Drop to pH value of solution value 6.7; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 2 times of weight portions of colloidal aluminum adding of 1 part, 1800 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 66 ℃ of water-baths heating;
With the liquid derivant that makes to the field planting leaf fall period kyoto grape tree spray, the vine acrial part is sprayed 1 part of liquid derivant with 500 parts of per hectare biomasss induces processing; 24 hours processing times; HPLC records the blade Resveratrol content and is elevated to 2 times, and the average Resveratrol content fresh weight ratio of blade reaches 82 μ g/g, and the fruit Resveratrol content is elevated to 2 times.
Embodiment 6
40 parts NaOH are dissolved in 1000 parts the water; AlCl with 160 parts
36H
2O is dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1800 rev/mins of mixing speeds fast 180ml/ minute; 67 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 70ml/ minute; Drop to pH value of solution value 5.7; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 100 times of weight portions of colloidal aluminum adding of 1 part, 800 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 60 ℃ of water-baths heating;
8 parts of the liquid derivants that makes are sprayed onto 80 portions of sub-grapes of shoot growth phase mare's milk to exsomatize and to induce processing on leaf, stem and the fruit; 10 hours processing times; HPLC records the blade Resveratrol content and is elevated to 3 times, and the average Resveratrol content fresh weight ratio of blade reaches 688 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 2 times, is tried frost-resistant mould in the strain;
Embodiment 7
40 parts NaOH are dissolved in 1000 parts the water; AlCl with 250 parts
36H
2O is dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1900 rev/mins of mixing speeds fast 240ml/ minute; 69 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 85ml/ minute; Drop to pH value of solution value 5.5; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 0 times of weight portion of colloidal aluminum adding of 1 part, 1200 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 65 ℃ of water-baths heating;
5 parts of the liquid derivants that makes are sprayed onto vow that Fu Luosha exsomatizes 100 parts of flowering stage and induce processing on grape leaf, stem and the fruit; 24 hours processing times; HPLC records the blade Resveratrol content and is elevated to 3 times, and the average Resveratrol content fresh weight ratio of blade reaches 119 μ g/g, and the stem Resveratrol content is elevated to 5 times, and the fruit Resveratrol content is elevated to 2 times;
Embodiment 8
40 parts NaOH are dissolved in 1000 parts the water; AlCl with 120 parts
36H
2O is dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1200 rev/mins of mixing speeds fast 160ml/ minute; 62 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 60ml/ minute; Drop to pH value of solution value 5.9; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 80 times of weight portions of colloidal aluminum adding of 1 part, 2000 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 62 ℃ of water-baths heating;
10 parts of the liquid derivants that makes are sprayed onto 60 parts of seedless purples of Berry Growth phase to exsomatize and to induce processing on grape leaf, stem and the fruit; 12 hours processing times; HPLC records the blade Resveratrol content and is elevated to 10 times, and the average Resveratrol content fresh weight ratio of blade reaches 310 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 2 times;
Embodiment 9
40 parts NaOH are dissolved in 1000 parts the water; 80 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1200 rev/mins of mixing speeds fast 200ml/ minute; 65 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 75ml/ minute; Drop to pH value of solution value 6.8; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 50 times of weight portions of colloidal aluminum adding of 1 part, 1200 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 60 ℃ of water-baths heating;
2 parts of the liquid derivants that makes are sprayed onto 150 parts of berry maturing stage Cabernet Sauvignons to exsomatize and to induce processing on grape leaf, stem and the fruit; 18 hours processing times; HPLC records the blade Resveratrol content and is elevated to 3 times, and the average Resveratrol content fresh weight ratio of blade reaches 90 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 1.5 times;
Embodiment 10
40 parts NaOH are dissolved in 1000 parts the water; 60 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 800 rev/mins of mixing speeds fast 120ml/ minute; 60 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 50ml/ minute; Drop to pH value of solution value 6.5; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 20 times of weight portions of colloidal aluminum adding of 1 part, 900 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 70 ℃ of water-baths heating;
10 parts of the liquid derivants that makes are sprayed onto 20 parts of huge peaks of leaf fall period to exsomatize and to induce processing on grape leaf, stem and the fruit; 20 hours processing times; HPLC records the blade Resveratrol content and is elevated to 2 times, and the average Resveratrol content fresh weight ratio of blade reaches 80 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 1.2 times;
Embodiment 11
40 parts NaOH are dissolved in 1000 parts the water; 50 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1500 rev/mins of mixing speeds fast 250ml/ minute; 70 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 100ml/ minute; Drop to pH value of solution value 6.0; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Get the water of 10 times of weight portions of colloidal aluminum adding of 1 part, 800 rev/mins of mixing speeds, colloid-stabilised then the use as liquid derivant treated in 65 ℃ of water-baths heating;
1 part of the liquid derivant that makes is sprayed onto on seedless white exsomatize grape leaf, stem and the fruits of 200 parts of leaf fall periods induces processing; 22 hours processing times; HPLC records the blade Resveratrol content and is elevated to 3 times, and the average Resveratrol content fresh weight ratio of blade reaches 21 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 1.1 times;
Embodiment 12
40 parts NaOH are dissolved in 1000 parts the water; 80 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1200 rev/mins of mixing speeds fast 200ml/ minute; 65 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 75ml/ minute; Drop to pH value of solution value 6.8; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out atomized drying in 60 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
The dry powder derivant that makes is sprayed field planting shoot growth phase American red globe grape tree, the vine acrial part is sprayed 1 part of dry powder derivant with 5000 parts of per hectare biomasss induce processing; 10 hours processing times; HPLC records the blade Resveratrol content and is elevated to 3 times, and the average Resveratrol content fresh weight ratio of blade reaches 75 μ g/g, and the fruit Resveratrol content is elevated to 2 times.
Embodiment 13
40 parts NaOH are dissolved in 1000 parts the water; 60 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 800 rev/mins of mixing speeds fast 120ml/ minute; 60 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 50ml/ minute; Drop to pH value of solution value 6.5; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out bake drying in 80 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
The dry powder derivant that makes is vowed that to field planting the Fu Luosha vine sprays flowering stage, the vine acrial part is sprayed 0.5 part of dry powder derivant with 5000 parts of per hectare biomasss induce processing; 15 hours processing times; HPLC records the blade Resveratrol content and is elevated to 2 times, and the average Resveratrol content fresh weight ratio of blade reaches 85 μ g/g.
Embodiment 14
40 parts NaOH are dissolved in 1000 parts the water; 50 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1500 rev/mins of mixing speeds fast 250ml/ minute; 70 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 100ml/ minute; Drop to pH value of solution value 6.0; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out atomized drying in 90 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
With the dry powder derivant that makes to the field planting Berry Growth phase seedless purple grape tree spray, the vine acrial part is sprayed 0.2 part of dry powder derivant with 5000 parts of per hectare biomasss induces processing; 18 hours processing times; HPLC records the blade Resveratrol content and is elevated to 5 times, and the average Resveratrol content fresh weight ratio of blade reaches 161 μ g/g.
Embodiment 15
40 parts NaOH are dissolved in 1000 parts the water; 90 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 2000 rev/mins of mixing speeds fast 250ml/ minute; 70 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 100ml/ minute; Drop to pH value of solution value 5.5; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out bake drying in 98 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
The dry powder derivant that makes is sprayed field planting berry maturing stage cabernet sauvignon grape tree, the vine acrial part is sprayed 0.1 part of dry powder derivant with 5000 parts of per hectare biomasss induce processing; 20 hours processing times; HPLC records the blade Resveratrol content and is elevated to 2 times, and the average Resveratrol content fresh weight ratio of blade reaches 71 μ g/g, and the fruit Resveratrol content is elevated to 2 times.
Embodiment 16
40 parts NaOH are dissolved in 1000 parts the water; AlCl with 90 parts
36H
2O is dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1000 rev/mins of mixing speeds fast 140ml/ minute; 65 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 55ml/ minute; Drop to pH value of solution value 6.7; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out atomized drying in 60 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
With the dry powder derivant that makes to the field planting leaf fall period kyoto grape tree spray, the vine acrial part is sprayed 5 parts of dry powder derivants with 5000 parts of per hectare biomasss induces processing; 24 hours processing times; HPLC records the blade Resveratrol content and is elevated to 1.5 times, and the average Resveratrol content fresh weight ratio of blade reaches 62 μ g/g.
Embodiment 17
40 parts NaOH are dissolved in 1000 parts the water; AlCl with 120 parts
36H
2O is dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1200 rev/mins of mixing speeds fast 160ml/ minute; 62 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 60ml/ minute; Drop to pH value of solution value 5.9; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out bake drying in 80 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
The dry powder derivant that makes is sprayed field planting shoot growth phase U.S. vine, the vine acrial part is sprayed 10 parts of dry powder derivants with 5000 parts of per hectare biomasss induce processing; 10 hours processing times; HPLC records the blade Resveratrol content and is elevated to 5 times, and the average Resveratrol content fresh weight ratio of blade reaches 139 μ g/g, and the fruit Resveratrol content is elevated to 3 times.
Embodiment 18
40 parts NaOH are dissolved in 1000 parts the water; AlCl with 250 parts
36H
2O is dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1900 rev/mins of mixing speeds fast 240ml/ minute; 69 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 85ml/ minute; Drop to pH value of solution value 5.5; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out bake drying in 98 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
1 part of the dry powder derivant that makes is sprayed onto 800 parts of shoot growth phase American Red to be lifted from body grape leaf, stem and the fruit and induces processing; 10 hours processing times; HPLC records the blade Resveratrol content and is elevated to 2 times, and the average Resveratrol content fresh weight ratio of blade reaches 50 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 1.1 times;
Embodiment 19
40 parts NaOH are dissolved in 1000 parts the water; AlCl with 120 parts
36H
2O is dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1200 rev/mins of mixing speeds fast 160ml/ minute; 62 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 60ml/ minute; Drop to pH value of solution value 5.9; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out atomized drying in 60 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
0.5 part of the dry powder derivant that makes is sprayed onto vows that Fu Luosha exsomatizes 1600 parts of flowering stage and induce processing on grape leaf, stem and the fruit; 24 hours processing times; HPLC records the blade Resveratrol content and is elevated to 1.5 times, and the average Resveratrol content fresh weight ratio of blade reaches 65 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 1.3 times;
Embodiment 20
40 parts NaOH are dissolved in 1000 parts the water; 80 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1200 rev/mins of mixing speeds fast 200ml/ minute; 65 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 75ml/ minute; Drop to pH value of solution value 6.8; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out bake drying in 80 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
0.1 part of the dry powder derivant that makes is sprayed onto 2000 parts of seedless purples of Berry Growth phase to exsomatize and to induce processing on grape leaf, stem and the fruit; 12 hours processing times; HPLC records the blade Resveratrol content and is elevated to 2 times, and the average Resveratrol content fresh weight ratio of blade reaches 55 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 1.1 times;
Embodiment 21
40 parts NaOH are dissolved in 1000 parts the water; 60 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 800 rev/mins of mixing speeds fast 120ml/ minute; 60 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 50ml/ minute; Drop to pH value of solution value 6.5; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out atomized drying in 90 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
2 parts of the dry powder derivants that makes are sprayed onto 400 parts of berry maturing stage Cabernet Sauvignons to exsomatize and to induce processing on grape leaf, stem and the fruit; 18 hours processing times; HPLC records the blade Resveratrol content and is elevated to 2 times, and the average Resveratrol content fresh weight ratio of blade reaches 68 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 2 times;
Embodiment 22
40 parts NaOH are dissolved in 1000 parts the water; 50 parts anhydrous Aluminum chlorides are dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1500 rev/mins of mixing speeds fast 250ml/ minute; 70 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 100ml/ minute; Drop to pH value of solution value 6.0; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out bake drying in 98 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
5 parts of the dry powder derivants that makes are sprayed onto 400 parts of huge peaks of leaf fall period to exsomatize and to induce processing on grape leaf, stem and the fruit; 20 hours processing times; HPLC records the blade Resveratrol content and is elevated to 2 times, and the average Resveratrol content fresh weight ratio of blade reaches 80 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 2 times;
Embodiment 23
40 parts NaOH are dissolved in 1000 parts the water; AlCl with 160 parts
36H
2O is dissolved in 1000 parts the water; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially dripped 1800 rev/mins of mixing speeds fast 180ml/ minute; 67 ℃ of water-baths heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 70ml/ minute; Drop to pH value of solution value 5.7; Stop to stir, left standstill 24 hours, treat that colloid forms and stable after to get colloidal aluminum subsequent use;
Colloidal aluminum is carried out atomized drying in 90 ℃, remove moisture after, cross 100 eye mesh screens, the dry powder derivant;
10 parts of the dry powder derivants that makes are sprayed onto on seedless white exsomatize grape leaf, stem and the fruits of 200 parts of leaf fall periods induce processing; 22 hours processing times; HPLC records the blade Resveratrol content and is elevated to 4 times, and the average Resveratrol content fresh weight ratio of blade reaches 30 μ g/g, and the stem Resveratrol content is elevated to 3 times, and the fruit Resveratrol content is elevated to 1.2 times.
Claims (2)
1. method of grape Resveratrol content that raises is characterized in that taking method following:
The preparation of a, derivant: the NaOH of 40 weight portions is dissolved in the water of 1000 weight portions and makes sodium hydroxide solution; With the anhydrous Aluminum chloride of 50-100 weight portion or the AlCl of 90-250 weight portion
36H
2O is dissolved in the water of 1000 weight portions and makes liquor alumini chloridi; Sodium hydroxide solution is splashed in the liquor alumini chloridi, initially drip speed and be not less than 100ml/ minute, mixing speed 800-2000 rev/min; 60-70 ℃ of water-bath heating when sodium hydroxide solution consumption during near aluminium chloride volume 3/4, is dripped speed and is reduced to 50-100ml/ minute; Survey the pH value between 5.5-6.8; Stop to stir, left standstill 24 hours, it is subsequent use to treat to get after colloid-stabilised colloidal aluminum;
The dilution of b, derivant: select the water of the 0-100 times of weight portion of colloidal aluminum adding of 1 weight portion, mixing speed 800-2000 rev/min, colloid-stabilised then the use as liquid derivant treated in 60-70 ℃ of water-bath heating;
C, the preparation of dry powder derivant: the colloidal aluminum that step a makes is carried out drying through spraying or drying mode, and the dry run temperature behind the removal moisture, is crossed 100 eye mesh screens less than 100 ℃, gets the dry powder derivant;
D, induce processing: the liquid derivant that above-mentioned steps b is made sprays vine, the vine acrial part is sprayed the liquid derivant of 1-10 weight portion with per hectare biomass 500 weight portions induce processing; The liquid derivant 1-10 weight portion that above-mentioned steps b is made is sprayed onto the 20-200 weight portion and exsomatizes and induce processing on grape leaf, stem and the fruit; The dry powder derivant that above-mentioned steps c is made sprays vine, the vine acrial part is sprayed 0.1-10 weight portion dry powder derivant with 5000 parts of per hectare biomasss induce processing; The dry powder derivant 0.1-10 weight portion that above-mentioned steps c is made is sprayed onto the 200-2000 weight portion and exsomatizes and induce processing on grape leaf, stem and the fruit; Above-mentionedly induce the processing time to be not less than 10 hours.
2. the method for a kind of grape Resveratrol content that raises according to claim 1 is characterized in that described derivant preparation and the used water of dilution step are any or mixing of running water, distilled water, deionized water of pH＜7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105090468A CN102047827B (en) | 2010-10-18 | 2010-10-18 | Method for promoting content of grape resveratrol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105090468A CN102047827B (en) | 2010-10-18 | 2010-10-18 | Method for promoting content of grape resveratrol |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102047827A CN102047827A (en) | 2011-05-11 |
| CN102047827B true CN102047827B (en) | 2012-05-09 |
Family
ID=43952898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105090468A Expired - Fee Related CN102047827B (en) | 2010-10-18 | 2010-10-18 | Method for promoting content of grape resveratrol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102047827B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102835263B (en) * | 2012-10-09 | 2014-07-30 | 张波 | Method for inducing accumulation of stilbenes in grapes |
| CN106105688A (en) * | 2016-06-30 | 2016-11-16 | 浙江森禾种业股份有限公司 | Red gloomy Rhizoma et radix veratri (Radix Rhizoma Veratri) cuttage breeding method |
| US11419277B2 (en) * | 2018-10-23 | 2022-08-23 | Seoul Viosys Co., Ltd. | Plant cultivation method and light treatment unit for increasing of the content of phytochemical |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090191294A1 (en) * | 2007-04-17 | 2009-07-30 | Ahmad Alkayali | Grape cane product, and method of making |
-
2010
- 2010-10-18 CN CN2010105090468A patent/CN102047827B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102047827A (en) | 2011-05-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105660642B (en) | Anticracking antistaling agent and the preparation method and application thereof before a kind of lichee is adopted | |
| CN103875722B (en) | Chinese herbal medicine merges the botanical fungicide extracted | |
| CN103571613A (en) | Preparation method of vitex negundo linn volatile oil | |
| CN102047827B (en) | Method for promoting content of grape resveratrol | |
| Nicasio-Torres et al. | Production of chlorogenic acid and isoorientin hypoglycemic compounds in Cecropia obtusifolia calli and in cell suspension cultures with nitrate deficiency | |
| CN107467077A (en) | A kind of fruits and vegetables bactericide of extract solution containing cake of camellia oleifera seeds and its application | |
| AL-Ghudani et al. | Determination of total phenolics, flavonoids and antioxidant activity of root crude extracts of Adenium obesum traditionally used for the treatment of bone dislocations and rheumatism | |
| JP5628639B2 (en) | Manufacturing method of Sendan extract | |
| CN106613719A (en) | High-yield grape planting method | |
| CN107517955B (en) | Composite rice seed life prolonging agent and preparation method and application thereof | |
| CN103689001B (en) | A kind of sterilant containing Hairy Waxmyrtle litter extracting solution and purposes thereof | |
| CN107162782A (en) | Clover specific complex sterilizing concentrated nutrient solution and preparation method thereof | |
| CN105638435B (en) | Utilize the method that honeybee is Crops ' pollination | |
| CN105237364B (en) | A kind of preparation method and application of trans-anethole | |
| CN108888995B (en) | Bamboo vinegar directional extraction method | |
| CN107751252B (en) | Application of Ledum palustre branch and leaf total volatile oil in prevention and control of stored grain pests | |
| CN113559025A (en) | Extraction method of cherry blossom cell water and application of obtained cherry blossom cell water | |
| CN109576060A (en) | A kind of natural aromatic object extraction process based on Anji white tea | |
| Lama et al. | Metabolic responses to in vitro in drought-tolerant in a Cucurbita pepo L. elicited by salicylic acid and zinc oxide nanoparticles | |
| CN109748734A (en) | One kind containing fruits and vegetables Chinese herbal medicine liquid fertilizer and its production technology | |
| Mengual et al. | Influence of abscisic acid and other plant growth regulators on citrus defense mechanisms to salt stress | |
| Ali | Role of salicylic acid in pre-and post-harvest attributes in horticulture | |
| CN105112167A (en) | Extraction method of osmanthus essential oil | |
| CN106942249A (en) | A kind of prevention and controls of Dian clove droop | |
| CN106723206B (en) | Method for improving flavonol content and oxidation resistance of grape fruits |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120509 Termination date: 20171018 |